NIH Common Fund Glycoscience Program

Participating Investigators Meeting

July 2-3, 2018
Natcher Conference Center

Agenda

July 2, 2018
Room E1/E2
8:00 a.m., Welcome & Opening Remarks
Martha Somerman, Director, NIDCR
Lawrence Tabak, Principal Deputy Director, NIH

Session I – Methods, Tools & Technologies

Chair, Dr. Pamela Marino, NIGMS

8:30 a.m., Drug-Like Modulators Targeting O-Glycosylation by GalNAc Transferase-2/3

Dr. Adam Linstedt, Carnegie Mellon University

8:40 a.m., GlycoMIr: Mapping the miRNA-glycogene interactome

Dr. Lara Mahal, New York University

8:50 a.m., Photocrosslinking probes to discover glycan-dependent interactions

Dr. Jennifer Kohler, UT South Western Medical Center

9:00 a.m., Chemical Genetic Tools for the Spatial and Temporal Modulation of O-GlcNAcylation
Dr. Natasha Zachara, Johns Hopkins University

9:10 a.m., BapaFPs: Genetically Encoded Fluorescent Indicators to Image Live-cell Nucleotide
Sugars
Dr. Huiwang Ai, University of Virginia

9:15 a.m., Metabolic carbohydrate cell wall probes for bacterial structure and immune recognition
studies
Dr. Catherine Leimkuhler Grimes, University of Delaware

9:25 a.m., Enabling tools for protists pathogen glycobiology

Dr. Christopher West* Dr. Rick Tarleton, Dr. Lance Wells, University of Georgia

9:35 a.m., Mining the Oral Microbiome for Novel Glycan-Binding Molecules
Dr. Stefan Ruhl, State University of New York Buffalo and Dr. Paul Sullam, University of
California San Francisco & San Francisco Veterans Administration Medical Center

9:45 a.m., Development of Multifunctional Probes for Profiling Microbial Glycans

Dr. Laura Kiessling* and Dr. Barbara Imperiali, Massachusetts Institute of Technology
9:50 a.m., Novel Methods and Technologies for Synthesis of Biomedically Relevant Carbohydrates
Dr. David Crich, Wayne State University, Dr. Peter Andreana University of Toledo

10:00 a.m., Chemoenzymatic synthesis of bacterial polysaccharides

Dr. Hai Yu* University of California Davis, Dr. Laura Kiessling, Massachusetts Institute of
Technology, Dr. Lei Li, Georgia State University

10:20 a.m., Picture

10:30 a.m., Break

Session II – Methods, Tools & Technologies

Glycosaminoglycans, Glycolipids & O-Glycomics
Chair, Dr. Karl Kruger, NCI

11:00 a.m., Expedited Synthesis of Glycosaminoglycans Containing Defined Sulfation Domains

Dr. Linda Hsieh-Wilson* California Institute of Technology, Dr. Xuefei Huang, Michigan
State University

11:10 a.m., Sequencing Glycosaminoglycans using Recognition Tunneling Nanopores

Dr. Xu Wang, Arizona State University

11:20 a.m., Detection and Histopathology Localization of O-Glycans and Glycosaminoglycans in

Tissues
Dr. Richard Drake, Medical University of South Carolina

11:30 p.m., An Automated Platform for the CE-MS Analysis of Glycosaminoglycans

Dr. Jonathan Amster, University of Georgia

11:40 a.m., Software for automated interpretation of heparan sulfate tandem mass spectra
Dr. Joseph Zaia, Boston University Medical Campus

11:45 p.m., Manipulating Glycosaminoglycans Using Synthetic Xylosides to Regulate Angiogenesis

Dr. Kuberan Balagurunathan, University of Utah

11:50 p.m., Facile chemoenzymatic synthesis and purification of glycolipids

Dr. Xi Chen* UC Davis; and Dr. George Peng Wang, Georgia State University

12:00 p.m., Development of high-throughput workflow for glycosphingolipid analysis and annotation
Dr. Rene Ranzinger, and Dr. Kazuhiro Aoki, University of Georgia

12:10 p.m., Production of high affinity anti-glycan and anti-glycolipid antibodies

Dr. Luc Teyton, Scripps Research Institute

12:20 p.m., Novel Technologies for Quantitative O-glycomics and Amplification/Preparation of

Cellular O-Glycans
Dr. Zhonghua Li for Dr. David Smith, Emory University
 NIH Common Fund Glycoscience Program
Participating Investigators Meeting
July 2-3, 2018
Natcher Conference Center

12:30 p.m., Working Lunch

Introductions of Staff, Synergistic CF Programs & Other Federal Opportunities:
Big Data & Glycoscience, Dr. Melissa St. Amand, DARPA
Human BioMolecular Atlas Program, Dr. Ananda Roy, OD
Human Milk Composition Data Base, Dr. Kellie Casavale, OS/OASH
Glycobiology of Alzheimer’s, Dr. Austin Yang, NIA
Big Data to Knowledge, Dr. Susan Gregurick
CF-GSP Working Group Introductions:
Dr. Houmam Araj, NEI; Mr. Tony Casco, OD; Dr. Preethi Chander, NIDCR; Dr.
Amy Krafft, NIAID; Dr. Karl Krueger, NCI; Dr. Angela Malaspina, NIAID; Dr.
Pamela Marino, NIGMS; Dr. Amanda Melillo, NIGMS; Dr. Mercy Prabhudas,
NIAID; Dr. Daniel Raiten, NICHD, Dr. Ananda Roy, OD; Dr. Rao Rapaka, NIDA; Dr.
Rita Sarkar, NHLBI; Dr. Salvatore Sechi, NIDDK; Dr. Douglas Sheeley, NIGMS; Dr.
Jill Beaver, OD; Dr. Bernadette Tyree, NIAMS; Dr. Austin Yang, NIA; Dr. Linda
Duffy, NCCIH.

Session III – Methods, Tools & Technologies

Automation of Glycan Synthesis
Chair, Dr. Nicola Pohl Indiana University

1:50 p.m., Sugar Building Blocks and Automated Synthesis of Biomedically-Relevant Glycans
Dr. Nicola Pohl, Indiana University

2:00 p.m., Streamlining the chemoenzymatic synthesis of asymmetrical glycans of biological

importance
Dr. Geert-Jan Boons, University of Georgia

2:10 p.m., Refinement and implementation of the automated oligosaccharide synthesizer

Dr. Alexei Demchenko* and Dr. Keith Stine, University of Missouri St Louis
Cristina De Meo Southern Illinois University-Edwardsville

2:20 p.m., Facile Synthesis of O-Glycans and O-Glycopeptides

Dr. George Peng Wang* and Dr. Lei Li, Georgia State University

2:30 p.m., Discussion of Automation: What are the key building blocks and enzymes needed for
chemoenzymatic automation (bacterial vs human enzymes)? What new developments
in artificial intelligence and deep learning could be applied to generate algorithms to
predict what sequences can easily be made by automated synthesis?
What standard set of building blocks should be utilized for assessing
rigor/reproducibility across chemical platforms? How are we cross validating?
Session IV – Methods, Tools & Technologies
Glycan Analysis: Progress Towards Facile Glycoproteomics
Chair, Dr. Karl Krueger, NCI

3:00 p.m., Making glycoproteomics via mass spectrometry more accessible to the greater scientific
community
Dr. Marc Dreissen for Dr. Carolyn Bertozzi, Sharon Pitteri, Stanford University
Stanford University

3:10 a.m., Multiplex Chemical Tags for High Throughput Glycan and Glycopeptide Quantitation
and Characterization
Dr. Lingjun Li, University of Wisconsin

3:20 p.m., An open source software suite for processing glycomics and glycoproteomics mass
spectral data
Dr. Joseph Zaia, Boston University Medical Campus

3:30 p.m., Break

3:55 p.m., Glycan linkage and sequence plus determination of site of glycosylation by
permethylation of glycopeptides and MSn analysis in a one pot experiment
Dr. Parastoo Azadi, University of Georgia

4:05 p.m., LC-MS Analysis of Site Specific Protein Glycoforms

Dr. Rado Goldman, Georgetown University

4:10 p.m., Shaping MSn technology for high impact glycan sequencing
Dr. Vernon Reinhold, University of New Hampshire

4:20 p.m., Characterization of glycan isomers by trapped ion mobility spectrometry-electron

activated dissociation tandem mass spectrometry
Dr. Cheng Lin, Boston University Medical Campus

4:30 p.m., Discussion:

How do we democratize this?
Are inexpensive equipment add-ons available or can they be developed to assist
with analysis of specialized classes of glycans?
What software requirements and spectral libraries are needed to assist in the
analysis of glycans and their conjugates?
Will glycan standards or the ability to readily synthesize them facilitate entry of
proteomic researchers into this field?
What are the major hurdles that prevent Proteomics laboratories utilizing MS to
routinely analyze carbohydrates as well?

5:00 p.m., Adjourn to Dinner

 NIH Common Fund Glycoscience Program
Participating Investigators Meeting
July 2-3, 2017
Natcher Conference Center
Agenda
Closed to the public

July 3, 2018
Room E1/E2
Session V – Methods, Tools & Technologies
Affinity Probes, Libraries, & Screens
Chair, Dr. Richard Cummings, Beth Israel Deaconess Medical Center

8:30 a.m., Automated Microfluidic Selection of Aptamers against Carbohydrates

Dr. Milan Stojanovic, Dr. Qiao Lin, Columbia University Health Sciences

8:40 a.m., Smart Anti-Glycan Reagents to Generate the Human Glycome Atlas
Dr. Richard Cummings* Beth Israel Deaconess Medical Center
Dr. Raymond Mernaugh, Vanderbilt University

8:50 a.m., Multiplex Luminex glycan array for large scale analyses of glycan-binding proteins
Dr. Jin-Xiong She, Georgia Reagents Research Institute Inc

9:00 a.m., Sialoglycan-Recognizing Probes for Defining Sialoglycomes in Biological Systems

Dr. Ajit Varki, University of California San Diego
Dr. Colin Parrish, Cornell University

9:10 a.m., Glycan array for phenotype-driven capture and genotyping of viruses in primary isolates
Dr. Kamil Godula, and Dr. Pascal Gagneux, University of California San Diego

9:20 a.m., Adapting multiplex microspheres to analyze glycosylation features by flow cytometry
Dr. Loretta Yang, Lectenz Bio (formerly Glycosensors and Diagnostics)

9:30 a.m., Facile Chemical Methods for Reverse Synthesis and Large-Scale Production of
Biomedically Relevant Glycan Libraries
Dr. Xuezheng Song, Emory University

9:40 a.m., On-chip Glycan Analysis of Clinical Specimens

Dr. Brian Haab, Van Andel Research Institute

9:50 a.m., Break

Session V – Methods, Tools & Technologies
Glycoscience data integration, and analysis tools
Chair, Dr. Douglas Sheeley, NIDCR

10:20 a.m., Glycopeptide Identification with a Comprehensive Proteomics Search Engine

Dr. Marshall Bern, Protein Metrics, Inc

10:30 a.m., Tools to enable non-specialists to model glycoconjugates and glycan-protein

interactions
Dr. Robert Woods* and Dr. Lachele Foley, University of Georgia
GlyProbity: tools to curate glycan structure pre and post deposition in the PDB
Dr. Robert Woods* University of Georgia and Dr. Jasmine Young, Rutgers State
University of New Jersey

10:50 a.m., GlyGen - Glycoscience data integration: Data acquisition and standardization
Dr. Raja Mazumder, Georgetown University

11:05 a.m., GlyGen - Glycoscience data integration: Glycan structures in GlyGen

Dr. Nathan Edwards, Georgetown University

11:20 a.m., GlyGen - Glycoscience data integration: Data access and user interfaces
Dr. Rene Ranzinger, University of Georgia

11:35 a.m., Discussion

12: 00 p.m., Working Lunch - Further Discussion of Informatics

Session VI – Methods, Tools & Technologies

Chemical Synthesis of Glycans
Chair, Dr. Hien Nguyen, Wayne State University

1:00 p.m., Catalytic Methods for Stereoselective 1,2-Cis Glycosylation

Dr. Hien Nguyen, Wayne State University

1:10 p.m., Small-Molecule Catalysts for the Stereoselective Synthesis of Oligosaccharides

Dr. Samuel Levi for Dr. Eric Jacobsen, Harvard College

1:20 p.m., Reagent Control for Stereoselective Glycosylation Reactions

Dr. Clay Bennett, Tufts University

1:30 p.m., Transformative Methods for the Solid Phase Synthesis of Oligosaccharides
Dr. Matthew Brichacek, University of Maine Orono

1:40 p.m., Catalytic Methods for Building Block Assembly and for Stereoselective Glycosylation
Dr. John Montgomery and Dr. Pavel Nagorny, University of Michigan
 NIH Common Fund Glycoscience Program
Participating Investigators Meeting
July 2-3, 2017
Natcher Conference Center

1:50 p.m., Development of novel approaches for stereoselective construction of glycosidic linkages
Dr. Liming Zhang* University of California Santa Barbara, and Dr. Jianglong Zhu,
University of Toledo

2:00 p.m., Develop Catalytic Methods to Streamline the Assembly of Oligosaccharides

Dr. Weiping Tang, University of Wisconsin-Madison

2:10 p.m., New Catalytic Methods for the Synthesis of Biomedically Relevant Carbohydrates
Dr. Maciej Walczak, University of Colorado

2:20 p.m., Facile Synthesis of Glycosulfopeptides and Related Bioconjugates

Dr. Elliot Chaikof* and Dr. Richard Cummings, Beth Israel Deaconess Medical Center

2:30 p.m., Discussion: Bond Building, defining a focus for the program/field.
Given bond making varies from easy to quite difficult:
What bonds can we make?
What bonds do we need to make?
Are there common rules we can use for bond making?
Do we need a set of standard reactions for bench marking new reactivities?
If so should it be based on the linkage’s they can make, or against a given
set of the most efficient/facile glycosylations?

3:00 p.m., Adjournment of Meeting Participants

3:00 p.m., Executive session for staff and consultants

4:30 p.m., Executive session Adjourns