DA-2

1.In UV-Vis absorption, what do the following terms mean: Explain with an example from
biology.

1. Hyperchromicity
2. Hypochromicity
3. Bathochromic shift
4. Hypsochromic shift

Refer research journals to get the information about the above question ( Browse science
direct journals through egateway@vit.ac.in.

2.The electrospray negative ion spectrum of an oligonucleotide displays the following ions: 2903.8
(40 %), 2580.9 (67 %), 2323.7 (100 %), 2111.4 (71 %) and 1935.7 (38 %). What is the molecular
weight of this oligonucleotide derivative?

3.Using the details in the mass spectra provided, calculate the probable mass of the molecule.
X axis ( relative abundance %) and Y axis (m/z).