Clostridium difficile antigen GDH detection kit

Pathogen and product description

Clostridium difficile (C. difficile) is the major aetiolo- Clostridium difficile Glutamate Dehydrogenase
Deh
ehyd
ydro
yd roge
ro gena
genase
ase
gical agent of diarrhoea and colitis associated with (GDH) is an enzyme produced in large
arge quantities by
antobiotics. C. difficile is the most common cause all toxigenic and non-toxigenic strains, making it an
of health care-associated diarrhoea in developed excellent marker for the organism.
countries and is a major source of nosocomial mor-
CerTest Clostridium difficile antigen GDH card test
bidity and mortality worldwide.
offers a coloured chromatog raphic immunoassay
Disease due to C. difficile develops when the or- for the qualitative detection of Clostridium difficile
ganism is allowed to proliferate in the colon, most Glutamate Dehydrogenase (GDH) in stool samples.
commonly after antibiotic use has eliminated com-
peting flora.
 antigen
n GDH detection kit

Test procedure

step 1

Take out the cap of the stool collection tube step 2 Close the tube with the diluent and stool
and use the stick to pick up sample. Shake the tube in order to assure
sufficient sample quantity. good sample dispersion.

10 min.

appear.
Wait for coloured bands to appear Cut the end of the cap and dispense
4 drops into the “S” circular
Read the result at 10 minutes. step 4 window marked with an arrow. step 3

Interpretation of the results

Clinical Sensitivity and Specificity

Sensitivity Specificity PPV NPV

CerTest Clostridium difficile

antigen GDH vs C. DIFF >99% >99% >99% >99%
QUIK CHEK Complete®

CERTEST BIOTEC, S.L.

Pol. Industrial Río Gállego II, Calle J, Nº 1,
GDH15EN

50840, San Mateo de Gállego, Zaragoza (SPAIN)

www.certest.es