BioK Quick Quiz on Enzymes (2.

5)

BioKnowledgy Quick Quiz on Enzymes (2.5)

[13 marks]

1. Which of the following will cause an enzyme to permanently lose its properties?

I. Hydrolysis
II. Freezing to –20°C
III. Dissolving it in water

A. I only

B. II only

C. I and II only

D. I and III only

(Total 1 mark)

2. The graph below shows the effect of substrate concentration on enzyme activity. What conclusion can
be drawn about section X of the graph?

A. The enzyme has started to denature and the reaction slows down.

B. The reaction has finished and the substrate has been used up.

C. The enzyme is saturated and is working at its maximum reaction rate.

D. Some of the enzyme has been consumed and the reaction has reached a plateau.
(Total 1 mark)

3. Which variable has the least effect on enzyme activity?

A. Temperature

B. Light intensity

C. pH

D. Substrate concentration
(Total 1 mark)

http://bioknowledgy.weebly.com/ (Chris Paine)

BioK Quick Quiz on Enzymes (2.5)

4. (a) Define the term active site.

Active site is the region of the enzyme which its substrate binds to to catalyse the reaction.
(1)

(b) Explain enzyme-substrate specificity.

Enzyme-substrate specificity is the idea that each enzyme is compatible with just one substrate.
This is because the charge and shape of the active site of enzymes can only specifically fit
one type of substrate.
(3)

5. Describe the use of biotechnology in the production of lactose-free milk.

Milk containing lactose is poured through a tube of alginate balls to produce lactose-free milk.
Alginate balls contain lactase, which is an enzyme that hydrolyses lactose into glucose
and galactose. By pouring milk through alginate balls, lactase break down lactose, and the
product is lactose-free milk, which contains glucose and galactose instead of lactose.

(6)

http://bioknowledgy.weebly.com/ (Chris Paine)