NAME : BAIQ NOPITA HANDRA ENI

NIM : E1M018016

DETERMINE LEVELS OF Fe IN PELECING KANGKUNG

A. Purpose
To determine levels of Fe in Pelecing Kangkung

B. Tools and Materials

The tools used in this research are the Atomic Absorption Spectrophotometer (PG
InstrumentsnAA500), analytical balance (ADAM PW-254), sample containers, knives,
ovens, blenders, measuring flasks, beakers, Erlenmeyer, measuring pipettes, stirring rods,
bath electricity. Meanwhile, the materials used in this study were samples of kale, HNO3
(Merck), aquades, and pure standard solutions for Fe.

C. Experimental procedure
The work procedure carried out in this experiment begins by weighing 2 grams of
stems and leaves of kale that have been oven and mashed, in the already known weight of
erlenmeyer. Then 10 mL of HNO3 p.a. added to each of these erlenmeyers and leave for
± 24 hours, after which the sample is heated to a temperature of 100 C until yellow vapor
is not visible and the solution becomes clear. Next, measure the extract from heating as
much as 1 mL diluted with water to a volume of exactly 50 mL and shake it until it is
homogeneous and the results of the dilution are analyzed using AAS.

D. Results and Discussion

The determination of Fe content is carried out by various analytical methods,
including the atomic absorption spectrophotometer (AAS) method. To be able to use this
method, the digestion stage is first carried out. Digestion is a treatment to dissolve or
change the sample into a measurable form of material so that the elements in it can be
analyzed. In this experiment, wet digestion is used because in general wet digestion can
be used to determine elements with low concentrations.
At this stage of digestion, the sample is first dried in an oven with the aim of
removing the moisture content in the sample to be analyzed, then the sample to be
analyzed is mashed using a blender. Then added with a solution of HNO3 as a digestion
Pen, because theoretically HNO3 is a strong oxidizer and can accelerate the oxidation
reaction and can accelerate the digestion process. HNO3 solution can dissolve or destroy
the metals in the sample because nitric acid can stabilize the metals to be analyzed. After
that the solution is left overnight in order to dissolve and break organic bonds, then the
solution is heated to help speed up the process of dissolving or breaking organic bonds.
Furthermore, the sample was diluted and the results of the dilution were analyzed using
AAS. After that, analysis and calculation of the iron content contained in the kale stem
samples are carried out.

E. Conclusion
Based on the objectives and experimental procedures, it can be concluded that
there is an element of Fe in Pelecing Kangkung.