MD Entrance Coaching Notes for Biochemistry

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Murali Bharadwaz Classes for MD Entrance Coaching Programe

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AIIMS BIOCHEMISTRY NOTES
Major Topics Minor topics
1. vitamins 26. DNA Blot

2. chromatography 27. Galactosemia
3. DNA replication 28. gluconeogenesis
4. lipoproteins 29. ACID BASE
5. amino acid 30. Alkaptonuria
6. DNA Structure 31. HMP Shunt
7. enzymes 32. Oxygen dissociation curve
8. glycolysis 33. prion
9. mutation 34. apoptosis
10.fatty acid synthesis 35. Bilirubin
11.Insulin 36. ciliary structure
12.Electron transport chain 37. Creatinine
13.RNA 38. inheritance pattern
14. sphingolipids 39. Purine metabolism
15.hemoglobin 40. Toxin mechanism
16.hormone 41. Urea cycle
17.Transcription translation
18.cell membrane
19.citric acid cycle
20.Collagen
21.ketone bodies
22.Trace elements
23.genetic code
24.Glycogen metaboloism
25.protein structure
Topic -Vitamins
1. Vit K is needed for which of these post transational modification processes- (AIIMS
MAY 2001)
a) Methylation
b) Carboxylation
c) Hydroxylation
d) Transketolation
Ans. B
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2. Thiamine is not used in which of the following reactions (AIIMS MAY 2001)
a) Lactate to pyruvate
b) Alphaketoglutarate to succinyl CoA
c) Glucose to pentose
d) Oxidative decarboxylation of alpha keto amino acids.
Ans. A
3. The activity of the following enzyme is affected by biotin deficiency: (AIIMS MAY
2003)
a) Transketolase,
b) Dehydrogenase.
c) Oxidase.
d) Carboxylase.
Ans. D
4. Which of the following combinations of biologically active molecules does vitamin A

consists of (AIIMS MA Y 2004)
a) Retinol, retinal and retinoic acid
b) Retinal, retinal and tetrahydrofolate
c) Retinal, conjugase and retinoic acid
d) P ABA, retinal and retinaldehyde
Ans. A
5. Aprt from occurring in nucleic acids, pyrimidines are also found in (AIIMS NOV 2005)
a) Theophylline
b) Theobromine
c) Flavin mononucleotide
d) Thiamine
Ans. D
6. Which of the following is a component of the visual pigment rhodopsin - (AIIMS NOV
2005)
a) b-Carotene
b) Retinal
c) Retinol t
d) Retinoic acid
Ans. B
7. Vitamin A is stored mainly as retinol esters in - (AIIMS NOV 2005)

a) Kidney
b) Muscle
c) Liver
d) Retina
Ans. C
8.Vitamin A intoxication causes injury to (AIIMS MAY 2007)

a) Lysosomes
b) Mitochondria
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c) Endoplasmic reticulum
d) Microtubules
Ans. A
9.Active form of vitamin D is (AIIMS MAY 2007)

a) Cholecalciferol
b) 24, 25 (OH)2 vit-D
c) l,25(OH)2vit-D
d) 25-OH vit-D
Ans. C
10. Vitamin which is excreted in urine is : (AIIMS MAY 2007)

a) Vitamin A
b) Vitamin C
c) Vitamin D
d) Vitamin K
Ans. B
11. Vitamin K is required for (AIIMS NOV 2007)

a) Hydroxylation :
b) Chelation
c) Transamination
d) Carboxylation
Ans. D
12. Thiamine deficiency causes decreased energy production because(AIIMS MAY

2008)
a) It is required for the process of transamination
b) It is co-factor in oxidative reduction
c) It is co-enzyme for transketolase in pentose phosphate pathway
d) It is co-enzyme for pyruvate dehydrogenase.
Ans. D
13. All of the following structures about thiaminic is true? (AIIMS NOV 2008)
a) It is a co-enzyme of lactate dehydrogenase
b) Its deficiency is associated with scurvy
c) Its co-enzyme function is done by thiamine monophosphate
d) It is co-enzyme for pyruvate dehydrogenase and a-ketoglutarate dehydrogenase
Ans. D
14. Carboxylation of clotting factors by vitamin K is required to be biologically active.

Which of the following amino acid is carboxylated? (AIIMS NOV 2008)
a) Histidine
b) Histamine
c) Glutamate
d) Aspartate
Ans is C
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15. Which coenzyme is responsible for carboxylation reaction? (AIIMS NOV 2008)
a) Biotin
b) FAD
c) NADH
d) Thiamine pyrophosphate
Ans is A
16. Refsum's disease is due to deficiency of which of the following enzyme? (AIIMS
NOV 2008)
a) Malonate dehydrogenase ,
b) Thiophorase
c) Succinate thiokinase
d) Phytanic alpha oxidase
Ans is D
17.Thiamin requirement increases in excessive intake (AIIMS MAY 2009)

a) Carbohydrate
b) Amino acid
c) Fat
d) Lecithine
Ans is A
18. Which of the vitamin deficiency lead to lactic acidosis? (AIIMS NOV 2010)
a) Riboflavin
b) Thiamin
c) Niacin
d) Panthotheic acid
Ans B
19. Which of the following vitamins is necessary for energy generation? (AIIMS MAY
2014) '
a) Folic acid
b) Thiamin
c) Riboflavin
d) Pyridoxin
Ans B
20. Thiamine deficiency can cause lactic acidosis due to which enzyme dysfunction
(AIIMS MAY 2015)
a) PEPcarboxykinase
b) PDH
c) Pyruvate carboxylase
d) Pyruvate kinase
Answer: (b)
T opic -Cchromatography
21. In chromatography mass movements of the substances in due to : (AIIMS MAY

2001)
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a) Diffusion
b) Electrophoresis
c) Paper chromatography
d) Osmosis
Ans. A
22. In which type of chromatography the proteins are bound to another substance
(AIIMS MAY 2001)
a) Hydrophobic chromatography
b) Affinity chromatography
c) Paper chromatography
d) Gel chromatography
Ans. B
23. The following separation technique depends on the molecular size of protein:
(AIIMS NOV 2002)
(a) Chromatography on a carboxymethyl (CM) cellulose column
(b) Isoelectric focusing
(c) Gel filtration chromatography
(d) Chromatography on a diethyl amino ethyl (DEAE)cellulose column
Ans. C
24. The molecular weight of a protein can be determined by - (AIIMS NOV 2004)
a) Native Poly Acrylamide Gel Electrophoresis (PAGE)
b) Sodium Dodecyl Sulphate h-PAGE
c) Isoelectric focussing
d) Ion Exchange Chromatograph
Ans. B
25. Substitution of which one of the following amino acids in place of alanine would
increase the absorbance of protein at 280 nm (AIIMS NOV 2005)
a) Leucine
b) Arginine
c) Tryptophan
d) Prolein
Ans. C
26. Nephelometry is based on the principle of (AIIMS NOV 2007)

a) Light attenuated in intensity by scattering
b) Refraction of light
c) Reduced transmission of light
d) Filtration of solutes by kidney
Ans A
27.Replacing alanine by which amino acid will increase UV absorbance of protein at

280nm wavelength: (AIIMS NOV 2008)
a) Leucine
b) Proline
c) Arginine
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d) Tryptophan
Ans D
28.Which amino acid migrates fastest on paper chromatography on methylcellulose

medium? (AIIMS MA Y 2009)
a) Aspartic acid
b) V aline
c) Lysine
d) Glycine
Ans B
29. Protein is purified using ammonium sulfate by: (AIIMS MAY 2009)
a) Salting out
b) Ion exchange chromatography
c) Mass chromatography
d) Molecular size exclusion
Ans A
30. In CRP , the C stands for: (AIIMS NOV 2009)

a) Canavallin A
b) Cellular
c) Chondoitin sulfate
d) C polysaccharide of streptococcus
Ans D
31. Which statement is incorrect: (AIIMS NOV 2009)

a. Chemiluminescene: excited electron in higher orbit comes to lower orbit by emitting
energy in form of photon
b. Bioluminescence: is a form of chemiluminescence
c. Phosphorescence: is energy emitted following absorption of EM radiation
d. Electrochemiluminescence: is energy emitted by photon
Ans D
32. Methods of introducing gene in target cells are all except: (AIIMS NOV 2010)
a) Electroporation
b) Transfection
c) Site directed recombination
d) FISH
Ans D
33. Movement of protein from nucleus to cytoplasm can be seen by (AIIMS MAY 2011)
a) FISH
b) FRAP
c) Confocal microscopy
d) Electron microscopy
Ans B
34. Which of the following technique can be used for exact localization of a genetic
locus? (AIIMS NOV 2013)
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a) Chromosome painting
b) Western blot
c) Comparative genomic hybridization
d) FISH
Ans is D
35. Chromosomes are visualized through light microscope with resolution of: (AIIMS
NOV 2013)
a) 5 kb
b) 50 mb
c) 5 mb
d) 500 kb
Ans is C
T opic -DNA replication
36. In a DNA the coding region reads 5'-CGT-3'. This would code in the RNA as:-
(AIIMS MAY 2002)
a) S'-CGU-S1
b) 5'GCA-3'
c) 5-ACG-3'
d) 5-UGC-3'
Ans. A
37. Excessive ultraviolet (uv) radiation is harmful to life. The damage caused to the
biological systems by ultraviolet radiation is by (AIIMS MAY 2004)
a) Inhibition of DNA synthesis
b) Formation of thymidine dimmers
c) Ionization
d) DNA fragmentation
Ans. B
38. Xeroderma pigmentation is caused due to a group of closely related abnormalities in

-(AIIMS NOV 2005)
a) Mismatch repair
b) Base excision repair
c) Nucleotide excision rapair
d) SOS repair
Ans. C
39. A nucleic acid was analyzed and found to contain 32% adenine, 18% guanine, 17%
cytosine end 33% thymine. The nucleic acid must be: (AIIMS MAY 2006)
a) Single-stranded RNA
b. Single-stranded DNA.
c. Double-stranded RNA
d. Double stranded DNA
Ans. D
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40. For PCR which of the following is not required: (AIIMS MA Y 2007)
a) T aq polymerase
b) d-NTP
c) Primer
d) Radiolabelled DNA probe
Ans is D
41.SYBR Green Dye is used for: (AIIMS MAY 2007)

a) HPLC
b) Immunofluorescence
c) PCR
d) EUSA
Ans. C
42. Cation used in PCR is(AIIMS NOV 2007)

a) Calcium
b) Lithium
c) Magnesium
d) Sodium
Ans. C
43. In molecular cloning, Blue-white screening is used for: (AIIMS MAY 2008)
a) T o screen for recombinant vectors
b) T o detect gene mutations
c) T o identify desired chromosomal DNA insert in plasmid vectors
d) T o detect host DNA in situ
Ans C
44. After digestion by restriction endonucleases DNA strands can be joined again by
(AIIMS MAY 2010)
a) DNA polymerase
b) DNA ligase
c) DNA topoisomerase
d) DNA gyrase
Ans B
45. CAP in LAC operon is (AIIMS MAY 2011)
a) Positive regulator
b) Negative regulator '
c) Attenuation
d) Constitutive expression
Ans A
46. After digestion by restriction endonucleases DNA strands can be joined again by
(AIIMS MAY 2011)
a) DNA polymerase
b) DNA ligase
c) DNA topoisomerase
d) DNA gyrase
Ans B
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47. CAP in LAC operon is (AIIMS NOV 2011)

a) Positive regulator
b) Negative regulator .
c) Attenuation
d) Constitutive expression
Ans A
48. For PCR which of the following is not required: (AIIMS NOV 2011)
a) T aq polymerase
b) d-NTP
c) dideoxynucleotides
d) Magnesium
Ans is C
49. Real time PCR is used for: (AIIMS MAY 2013)

a) Multiplication of RNA
b) Multiplication of Proteins
c) Multiplication of specific segments of DNA
d) T o know how much amplification of DNA has occurred
Ans is D
50. Real time PCR is used for: (AIIMS NOV 2013)

a) Multiplication of RNA
b) Multiplication of Proteins
c) Multiplication of specific segments of DNA
d) T o know how much amplification of DNA has occurred
Ans is D
T opic -lipoproteins
51. Dietary triglycerides are transported by (AIIMS MAY 2001)

a) Chylomicrons
b) VLDL
c) LDL
d) HDL
Ans. is A
52.Colonic bacteria, on digestion of dietary fibres would give- (AIIMS NOV 2001)
a. Free radicals
b. Glycerol
c. Butyrate
d. Sucrose
Ans. C
53. Cholesterol present in LDL : (AIIMS MAY 2003)

a) Represents primarily cholesterol that is being removed from peripheral cells.
b) Binds to a receptor and diffuses across the cell membrance.
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c) On accumulation in the cell inhibits replenishment of LDL receptors.
d) When enters a cell, suppresses activity of acyl- CoA: cholesterol acytranferase AC
AT .
Ans. C
54. All of the following statements about LDL are true except (AIIMS MAY 2004)
a) It delivers cholesterol to cells
b) It contains only one Apoprotein
c) It is a marker for cardiovascular disease
d) It contains Apo-B4
Ans. D
55. Increased level of lipoprotein(a) predisposes to (AIIMS NOV 2006)

a) Liver cirrhosis
b) Atherosclerosis
c) Nephrotic syndrome
d) Pancreatitis
Ans. B
56. Triglycerides are maximum in (AIIMS MAY 2007)

a) Chylomicrons
b) VLDL
c) LDL
d) HDL
Ans. A
57. Which receptors are present in liver for uptake of LDL: (AIIMS MAY 2009)
a) ApoE
b) Apo A and Apo E
c) Apo E and Apo B100
d) Apo B100
Ans is C
58. A patient was diagnosed with isolated increase in LDL. His father and brother had
the same disease with increased cholesterol. The likely diagnosis is: (AIIMS MAY
2009)
a) Familial type III hyperlipoproteinemia
b) Abetalipoproteinemia
c) Familial lipoprotein lipase deficiency (type I)
d) LDL receptor mutation
Ans is D
59. Apo B48 & Apo B100 is synthesized from the same mRNA; the difference between
them is due to: (AIIMS MAY 201 1)
a) RNA splicing'
b) Allelic exclusion
c) Deamination of cytidine to uridine
d) Upstream repression
Ans is C
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60. All the statements regarding LDL receptors are true except: (AIIMS NOV 2011)
a) Present only in the extra-hepatic tissues
b) Clathrin-coated pits contain the receptor on cell membrane
c) It is taken into the cells by endocytosis
d) Increased cellular cholesterol down-regulates the synthesis of LDL receptors
Ans is A
61. 7 yr old boy presents with severe abdominal pain. On examination he has
xanthomas. Blood drawn for work up has milky appearance of plasma. Which
lipoprotein is increased (AIIMS MAY 2015)
a) Chylomicron
b) Chylomicron remnants
c) LDL
d) HDL
Answer: A
62. In a person, LDL is highly elevated but the level of LDL receptors is normal. What
could be the cause (AIIMS MAY 2015)
a) Phosphorylation of LDL receptors
b) ApoB 100 mutation
c) Lipoprotein lipase deficiency
d) Lecithin Cholesterol Acyl transferase deficiency
Answer: B
63. If in a person, T otal Cholesterol= 300mg/dL, HDL= 25mg/ dL and TAG= 150mg/dL,
find out the value of LDL. (AIIMS MAY 2015)
a) 245
b) 125
c) 95
d) 55
Answer: A
T opic -Amino acid
64. Amino acids secreted in cystinuria are all except- (AIIMS MAY 2001)
a) Ornithine
b) Histidine
c) Arginine
d) Lysine
Ans. is B
65. Most stable amino acid at the physiological pH is (AIIMS MAY 2001)
a) Histidine
b) Lysine
c) Arginine
d) Glycine
Ans. is A
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66. Which of these amino acids does not have anomeric carbon atom:- (AIIMS MAY
2002)
a) V aline ?
b) Alanine
c) Tyrosine
d) Glycine
Ans. Is D
67. The presence of 2 extra pairs of electrons on the oxygen in watere molecule results
in : (AIIMS MAY 2002)
a) Makes water a non-polar solvent
b) Forms covalent bonds in the ice.
c) Electro negative charge on water molecule
d) Electro positive charge on water molecule
Ans. C
68. Which one of the following amino acids is most likely to be found in the
transmembrane region of a protein (AIIMS NOV 2004)
a) Lysine M
b) Arginine
c) Leucine
d) Aspartate
Ans. is C
69. At physiological pH, the carboxy-terminal of a peptide is (AIIMS NOV 2005)

a) Positively charged
b) Negatively charged
c) Neural
d) Infinitely charged
Ans. B
70. The amino acid producing ammonia in kidney is (AIIMS MAY 2007)
a) Glutamine
b) Alanine
c) Methionine
d) Glycine
Ans. is A
71.All of the following are essential aminoacids except (AIIMS MAY 2007)
a) Methionine
b) Lysine
c) Alanine
d) Leucine
Ans. is C
72. Different sequence of amino acids having similar structure of proteins is an example
of (AIIMS NOV 2007)
a) Divergence
b) Convergence
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c) Opportunistic
d) Incidental
Ans is B
73. All are true about glutathione except: (AIIMS MAY 2008)
a) It is a tripeptide
b) It converts hemoglobin to methemoglobin
c) It conjugates xenobiotics
d) It scavenges free radicals and superoxide ions
Ans. is B
74. All are true about glutathione except: (AIIMS NOV 2008)
a) It is a tripeptide
b) It converts hemoglobin to methemoglobin
c) It conjugates xenobiotics
d) It is co-factor of various enzymes
Ans. is B
75.NO is synthesized by: (AIIMS MAY 2009)

a) Uracil
b) Aspartate
c) Guanosine
d) Arginine
Ans is D
T opic -DNA Structure
76. At the physiological ph the DNA molecules are (AIIMS NOV 2002)
(a) Positively charged
(b) Negatively charged
(c) Neutral
(d) Amphipathic
Ans. B
77. At the physiological pH the DNA molecules are : (AIIMS MA Y 2003)

a) Positively charged.
b) Negatively charged.
c) Neutral.
d) Amphipathic
Ans. B
78. Steroids act via nuclear receptors which interact with DNA through (AIIMS NOV
2006)
a) Helix turn helix
b) Zinc finger motif
c) Histidine
d) Leucine zipper
Ans. is B
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78. Rapid method of chromosome identification in intersex is (AIIMS MA Y 2007)

a) FISH
b) PCR
c) SSCP
d) Karyotyping
Ans is A
79. Y-chromosome is: (AIIMS MAY 2007)

a) Metacentric :
b) Sub-metacentric
c) Acrocentric
d) Longer than the X-chromosome
Ans is C
80. Intron is not found in which DNA? (AIIMS NOV 2008)

a) Nuclear DNA
b) Mitochondrial DNA
c) B DNA
d) ZDNA
Ans is B
81. Karyotyping under light microscopy is done by: (AIIMS NOV 2009)
a. R banding
b. Q banding
c. G banding
d. C banding
Ans is C
82. Histone acetylation causes? (AIIMS MAY 201 1)

a) Increased Heterochromatin formation
b) Increased Euchromatin formation
c) Methylation of cystine
d) DNA replication
Ans is B
83. Triplex DNA is due to (AIIMS MAY 2011)

a) Hoogsteen pairing
b) Palindromic sequences
c) Large no. of guanosine repeats
d) Polypyramidine tracts
Ans is A
84. What is the percentage of coding DNA in genome? (AIIMS MAY 2014)
a) 0.5%
b) 1%
c) 1.5%
d) 2%
Ans is D
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T opic -Enzymes
85.Enzymes Ea,Eb and Ec are under allosteric control of S2 and PI and P2

respectively . If Ec is defective what would occur - (AIIMS NOV 2001)
a. Accumulation of SI
b. Accumulation of PI
c. Accumulation of S2
d. Decrease of S2
Ans. C
86. One of the following enzyme is not a protein: (AIIMS NOV 2002)
(a) DNAase
(b) Abzyme
(c) EcoRI
(d) Ribozyme
Ans. D
87. The type of enzyme inhibtion (in which succinate dehydrogenase reaction is
inhibited by malonate is an example of: (AIIMS MAY 2006)
a) Noncompetitive
b) Uncompetitive
c) Competitive
d) Allosteric
Ans. C
88. Chymotrypsinogen is a (AIIMS NOV 2006)

a) Zymogen
b) Carboxypeptidase
c) Transaminase
d) Elastase
Ans. is A
89. Non-functional enzymes are all except: (AIIMS NOV 2008)

a) Alkaline phosphatase
b) Acid phosphatase
c) Lipoprotein lipase
d) Gamma glutamyl transpeptidase
Ans is C
90.True about isoenzymes is: (AIIMS NOV 2011)

a) Same quaternary structure
b) Same distribution in different organs
c) Same enzyme classification with same numbers
d) Catalyze the same reaction
Ans is D
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91. All of the following are true regarding oxygenases except: (AIIMS NOV 201 1)
a) Incorporate 2 atoms of oxygen.
b) Incorporate 1 atom of oxygen.
c) Required for hydroxylation of steroids.
d) Required for carboxylation of drugs.
Ans is D
92. Chymotrypsinogen is a: (AIIMS NOV 201 1)

a) Transaminase
b) Carboxypeptidase
c) Elastase
d) Zymogen
Ans is D
93. Which of the following is a suicide enzyme? (AIIMS MAY 2013)

a) Lipoxygenase
b) Cyclooxygenase
c) 5' Nucleotidase
d) Thromboxane synthase
Ans is B
94. Which of the following is a suicide enzyme? (AIIMS NOV 2013)

a) Lipoxygenase
b) Cyclooxygenase
c) 5' Nucleotidase
d) Thromboxane synthase
Ans is B
T opic -Glycolysis
95.Cancer cells derive nutrition from (AIIMS NOV 2001)

a. Glycolysis
b. Oxidative phosphorylation
c. Increase in mitochondria
d. From a fast food joint
Ans. A
96. Which of the following does not cross the cell membrane:- (AIIMS MAY 2002)
a) Glucose-6-phosphate
b) Glucose
c) Nitrous oxide
d) Carbon monoxide
Ans. A
97. By which of the following anticoagulants used in estimating blood glucose, glycolysis
is prevented- (AIIMS NOV 2005)
a) EDTA
b) Heparin
c) Sodium fluoride
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d) Sodium citrate
Ans. C
98. Anticoagulant used to estimate glucose from a sample sent from PHC is (AIIMS
NOV 2006)
a) EDTA
b) Calcium oxalate
c) Potassium oxalate + NaF
d) Sodium citrate
Ans. is C
99. Enzyme responsible for complete oxidation of glucose to CO2 to water is present in
(AIIMS MAY 2007)
a) Cytosol
b) Mitochondria
c) Lysosomes
d) Endoplasmicreticulum
Ans. is B
100. Glycolysis occurs in (AIIMS MAY 2007)

a) Cytosol
b) Mitochondria
c) Nucleus
d) Lysosome
Ans. is A
101. Peroxidase enzyme is used in estimating (AIIMS NOV 2007)

a) Haemoglobin
b) Ammonia
c) Creatinine
d) Glucose
Ans is D
102. Anticoagulant used to estimate glucose from a sample sent from PHC is (AIIMS
NOV 2011)
a) EDTA
b) Calcium oxalate
c) Potassium oxalate + NaF
d) Sodium citrate
Ans. is C
103. Which of the following enzymes catalyze the irreversible steps of glycolysis?
(AIIMS MAY 2013)
a) Glucokinase, phosphofructokinase, pyruvate carboxylase
b) Hexokinase, fructose 1, 6 biphosphatase, pyruvate kinase
c) Glucokinaserphosphofructokinase, pyruvate kinase
d) Enolase, fructose 1, 6 biphospahatase, phosphofructokinase
Ans is C
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T opic -Mutation
104. A mutation in the codon which causes a change in the coded amino acid, is known
as:- (AIIMS MAY 2002)
a) Mitogenesis
b) Somatic mutation
c) Missense mutation
d) Recombination
Ans. C
105. In a mutation if valine is replaced by which of the following would not result in any
change in the function of protein:- (AIIMS MAY 2002)
a) Proline
b) Leucine
c) Glycine
d) Aspartic acid
Ans. D
106. An alfa helix of a protein is most likely to be disrupted if a missense mutation

introdues the following amino acid with in the alpha helical structure (AIIMS NOV
2002)
(a) Alanine
(b) Aspartic acid
(c) Tyrosine
(d) Glycine
Ans. B
107. Same aminoacid is coded by multiple codons d/t following: (AIIMS NOV 2006)
a) Degeneracy
b) Frame-shift mutation
c) Transcription
d) Mutation
Ans. is A
108.Which test uses oligomer with single base pair substitution: (AIIMS MAY 2009)
a) PCR
b) RFLP
c) Error coded mutation analysis
Ans is B
109. A four-year-old child is diagnosed with Duchenne muscular dystrophy, an X-linked

recessive disorder. Genetic analysis shows that the patient's gene for the muscle
protein dystrophin contains a mutation in its promoter region. What would be the
most likely effect of this mutation? (AIIMS NOV 2010)
a) T ailing of dystrophin mRNA will be defective.
b) Capping of dystrophin mRNA will be defective.
c) T ermination of dystrophin transcription will be deficient.
d) Initiation of dystrophin transcription will be deficient.
Ans is D
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110. Methylation of cytosine is associated with: (AIIMS MAY 2014)

a) Decreased expression of gene
b) Increased expression of gene
c) Mutation
d) No effect
Ans is A
11 1. All of the following are examples of uniparental disomy except: (AIIMS MAY 2014)
a) Bloom syndrome
b) Prader Willi syndrome
c) Angelman syndrome
d) Russel Silver syndrome
Ans is A
112. RNAi can cause the following in a gene (AIIMS MAY 2015)
a) Knock in
b) Knock out
c) Knock down
d) Knock up
Answer: C
T opic -fatty acid synthesis
113. In synthesis of fatty acids energy is supplied by:- (AIIMS MA Y 2002)

a) NAD
b) FAD
c) GTP
d) NADPH
Ans. D
114.Mitochondria is involved in all of the following, except: (AIIMS NOV 2003)

(a) ATP production
(b) Apoptosis
(c) Tricarboxylic acid cycle
(d) Fatty acid biosynthesis
Ans. is D
115. The most important source of reducing equivalents for fatty acid synthesis in the
liver is (AIIMS MAY 2004)
a) Glycolysis
b) TCA cycle
c) Uronic acid pathway
d) HMP pathway
Ans. D
116. Agranular cytoplasmic reticulum is involved in the synthesis of (AIIMS NOV 2006)
a) Protein
b) Lipid
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c) Vitamin
d) Carbohydrate
Ans. is B
117. Insulin causes lipogenesis by all except: (AIIMS MAY 2008)

a) Increasing acetyl-CoA carboxylase activity
b) Increases the transport of glucose into the cells
c) Inhibits pyruvate dehydrogenase
d) Decreases intracellular cAMP level
Ans. is C
118. In a well fed state, the activity of CPT-I in outer mitochondrial membrane is
inhibited by: (AIIMS NOV 2011)
a) Glucose
b) Pyruvate
c) Acetyl CoA
d) Malonyl CoA
Ans is D
119. Which of the following enzyme is not a component of fatty acid synthase complex?
(AIIMS NOV 2013)
a) Ketoacyl synthase
b) Thioesterase
c) Acetyl-CoA carboxylase
d) Enoyl reductase
Ans is C'
120. Brown adipose tissue is present in all except (AIIMS MAY 2015)
a) Around blood vessels
b) Subcutaneous tissue
c) Adrenal cortex
d) Scapula
Answer: B
T opic -Insulin
121. In the following food items, which one has the highest 'Glycemic Index1? (AIIMS
NOV 2003)
(a) Corn-flakes
(b) Brown rice
(c) Ice-cream.
(d) Whole wheat bread
Ans. is‘A
122.Insulin increases the activities of all of the following enzymes, EXCEPT (AIIMS
MAY 2004)
a) Glucokinase
b) Pyruvate carboxylase
c) Glycogen synthase
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d) Acetyl-CoA carboxylase
Ans. B
123. Glucose transporter in myocyte stimulated by insulin is: (AIIMS NOV 2009)
a. GLUT 1
b. GLUT 2
c. GLUT 3
d. GLUT 4
Ans is D
124. After overnight fasing, levels of glucose tranporters reduced in (AIIMS

MAY 2010)
a) Brain cells
b) RBCs
c) Adipocytes
d) Hepatocytes
Ans is C
125. Starting material for production of insulin from bacteria is: (AIIMS NOV 2011)
a) Genomic DNA from beta pancreatic cells of human
b) Genomic DNA from lymphocytes of human
c) mRNA from beta pancreatic cells of human
d) mRNA from lymphocytes of human
Ans. is C
126. Insulin resistance is seen in patients with liver disease due to: (AIIMS MAY 2013)
a) Damaged hepatocytes
b) Hepatic steatosis
c) Impaired insulin release
d) Low c peptide level
Ans is B
127. Increased ratio of insulin/glucagon causes- (AIIMS NOV 2013)

a) decreased amino acid synthesis
b) decreased level of lipoprotein
c) decreased ratio of cy-amp/5 amp
d) all
Ans is C
127. In fasting states RBC use (AIIMS MAY 2015)

a) Glucose
b) Alanine
c) Ketone body
d) Fatty acid
Answer: (a)
T opic -Electron transport chain
128. Which of the following membrane has the highest protein content per gram tissue:-
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(AIIMS MAY 2002)
a) Inner mitochondrial membrane
b) Outer mitochondrial membrane
c) Plasma membrane
d) Myelin sheath
Ans. is A
129. The electron flow in Cytochrome C oxidase can be blocked by: (AIIMS MAY 2006)
a) Rotenone
b) Antimycin-A
c) Cyanide
d) Actinomycin
Ans. (c)
130. All take place in mitochondria except (AIIMS MA Y 2007)

a) Fatty acid oxidation
b) EMP pathway
c) Electron transport chain
d) Citric acid cycle
Ans. is B
131. Fireflies produce light due to: (AIIMS NOV 2008)

a) NADH
b) GTP
c) ATP
d) Phosphocreatinine
Ans is C
132. Transport of ADP in and ATP out of mitochondria is inhibited by-(AIIMS NOV 2010)
a) Atractyloside
b) Oligomycin
c) Rotenone
d) Cyanide
Ans is A
133. Which of the following enzymes does not participate in oxidation-reduction

reactions? (AIIMS MAY 2013)
a) Oxygenases
b) Peroxidases '
c) Hydroperoxidases
d) Dehydrogenases
Ansis None
134. Last electron acceptor in electron transport chain is: (AIIMS MAY 2014)
a) NADH2
b) FADH2
c) O2
d) Cytochrome C
Ans is C
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T opic -RNA
135. Replication and transcription are similar processes in mechanistic terms because
both : (AIIMS MAY 2003)
a) Use RNA primers for initiation.
b) Use deoxyribonucleotides as precursors.
c) Are semi conserved events
d) Involve phosphodiester bond formation with elongation occurring in the 5' - 3'
direction.
Ans. D
136. Poly(A) tail translates into: (AIIMS MAY 2008)

a) Polyproline
b) Polylysine
c) Polyalanine
d) Polyglycine
Ans is B
137. Synthesis of an immunoglobulin in membrane bound or secretory form is

determined by: (AIIMS NOV 2008)
a) One turn to two turn joining rule
b) Class switching
c) Differential RNA processing
d) Allelic exclusion
Ans is C
138. Splicing activity is a function of: (AIIMS MAY 2010)

a) mRNA
b) snRNA
c) tRNA
d) rRNA
Ans is B
139. Gene duplication plays an important role in the evolution of- (AIIMS MAY 201 1)
a) m Rna
b) rRna
c) tRna
d) hnRna
Ans is A
140. Poly(A) tail on translation will give rise to: (AIIMS NOV 201 1)
a) Polyglycine
b) Polylysine
c) Polyalanine
d) Polyproline
Ans is B
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141. True about ribozymes is: (AIIMS NOV 2013)

a) Cut DNA at specific site
b) GTPase activity
c) Peptidyl transferase activity
d) Participate in DNA synthesis
Ans is C
T opic -Sphingolipids
142. Viscosity of synovial fluid depends upon:- (AIIMS MAY 2002)

a) N-acetyl galactosamine
b) N-acetyl glucosamine
c) Glucuronic acid
d) Hyaluronic acid
Ans. D
143. Accumulation of sphingomyelin in phagocytic cells is feature of (AIIMS NOV 2002)

(a) Gauchers disease
(b) Niemann pick ds
(c) T ay sachs disease
(d) Downs syndrome
Ans. B
144. Molecular change in Lysosomal storage disorder is (AIIMS NOV 2007)

a) Defective fusion of lysosomes and phagosomes
b) Increased synthesis of some substrates
c) Mutation of genes encoding lysosomal hydrolases
d) Membrane defect of lysosomes
Ans is C
145. Mucopolysaccharide hyaluronic acid is present in (AIIMS NOV 2006)

a) Vitreous humor
b) Cornea
c) Bloodvessel
d) Lens
Ans. is A
146. Phosphatidyl choline in a lipid monolayer, at pH of 3.5 leads to: (AIIMS MAY 2008)
a) Decreased surface potential
b) Increased surface potential
c) Decreased dipole moment
d) Zero dipole moment
Ans is ?
147. A 48 yr old lady presented with anaemia and had hepatosplenomegaly. On

histopathological examination crumpled tissue paper appearance is seen. Which is
the product likely to have accumulated (AIIMS MAY 2015)
a) Glucocerebroside
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b) Sphingomylin
c) Sulfatide
d) Ganglioside
Answer: (a)
148. A child comes with coarse facial features, big tongue, hepatosplenomegaly,
mucous discharge from nose. Diagnosis is (AIIMS MAY 2015)
a) Hurler's syndrome
b) Beckwith Weidemann syndrome
c) Hypothyroidism
d) Proteus syndrome
Answer: (a)
T opic -Hemoglobin
149.In hemoglobin the innate affinity of heme for carbon monoxide is diminished by the
presence of (AIIMS NOV 2002)
(a) HisF8
(b) HisE7
(c) GlyB6
(d) ThrC4
Ans. B
150. Hemoglobin electrophoresis is based on (AIIMS MAY 2007)

a) molecular weight
b) charge
c) solubility
d) calorimetric properties
Ans. is B
151.Hemoglobin estimation is done by all except – (AIIMS NOV 2007)

a) Drabkin's
b) Wintrobes
c) Sahli's
d) Spectrophotometry
Ans. is B
152. True about Sickle cell disease are all, Except: (AIIMS MAY 2010)
a. Single nucleotide change results in change of Glutamine to Valine
b. Sticky patch is generated as a result of replacement of a non polar residue with a
polar residue
c. HbS confers resistance against malaria heterozygotes
d. RFLP results from a single base change
Ans is B
153. True about Sickle cell disease are all, Except: (AIIMS MAY 201 1)
a) Single nucleotide change results in change of Glutamine to Valine
b) Sticky patch is generated as a result of replacement of a non polar residue with a
polar residue
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c) HbS confers resistance against malaria in heterozygotes
d) RFLP results from a single base change
Ans is B
154. Embryonic hemoglobin is mainly composed of: (AIIMS MAY 2013)

a) Alpha and gamma chains
b) Alpha and beta chains
c) Zeta and epsilon chains
d) Alpha and delta chains
Ans is C
T opic -Hormone
155. The following hormone does not have any intracellu- larreceptor (AIIMS NOV
2002)
(a) Vitamin D3
(b) Cortisone
(c) Adrenaline
(d) Thyroxine
Ans. C
156. Steroid hormone receptors have attachment site for all except: (AIIMS MAY 2008)
a) Steroid hormone
b) Transcription repressors
c) Hormone responsive element
d) Transcription activators
Ans is B
157. True about G protein coupled receptors is: (AIIMS MAY 2008)
a) G proteins bind to hormones on the cell surface
b) All the three subunits alpha, beta and gamma should bind to each other for G protein
to act
c) G proteins act as inhibitory and excitatory because of difference in alpha subunit
d) G protein is bound to GTP in resting state
Ans is C
158. Action of a-subunit of G protein is: (AIIMS NOV 2008)

a) Breakdown of GTP to GDP
b) Conversion of GDP to GTP
c) Internalization of receptors
d) Binding of agonist
Ans is A
159. Among the following all are hydrophilic hormones that act on cytosolic receptors
except one which is a lipophilic hormone that acts on nuclear receptor: (AIIMS
MAY 2011)
a) Thyroxine
b) Epinephrine
c) GH
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d) ACTH
Ans is A
160. Most common end product of progesterone metabolism found in urine is: (AIIMS
NOV 2013)
a) Pregnanetriol
b) Pregnenolone
c) Pregnanediol
d) 17 hydroxypregnenolone
Ans is C
T opic -Transcription translation
161. Secretory proteins are synthesized in - (AIIMS NOV 2005)

a) Cytoplasm
b) Endoplasmic Reticulum
c) First in cytoplasm and then in Endoplasmic Reticulum
d) First in Endoplasmic Reticulum and then in cytoplasm
Ans. is B
162. T o synthesize insulin on a large scale basis, the most suitable starting material
obtained from the beta cells of the pancreas is – (AIIMS NOV 2005)
a) Genomic DNA
b) T otal cellular RNA
c) cDNA of insulin
d) mRNA of insulin
Ans. . is D
163. The following is a generalized diagram of a typical eukaryotic gene:
What is the most likely effect of a 2bp insertion in the middle of the intron? (AIIMS MA Y
2006)
a) Normal transcription, altered tranlsation
b) Defective termination of transcription, normal translation
c) Normal transcription, defective Mrna splicing
d) Normal transcription, normal translation
Ans. A
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164. Ribosome has following enzymatic activity? (AIIMS NOV 2008)
a) Peptidyl transferase
b) Peptidase
c) Aminoacyl tRNA synthetase
d) GTPase
Ans is A
165. False about eukaryotic protein synthesis is: (AIIMS MAY 2009)
a) N formyl Met is the first t-RNA to come into action
b) mRNA read from 5' to 3'
c) Ef2 shifts between GDP to GTP
d) Capping helps in attachment of mRNA to 40 S ribosome
Ans. is A
166. Which of the following is not required for protein synthesis in eukaryotes: (AIIMS
NOV 2011)
a) RNA Polymerase
b) Ribosomes
c) Peptidyl transferase
d) Amino acyl tRNA synthetase
Ans is A
T opic -Cell membrane
167. RBC membrane is maintained by:- (AIIMS MAY 2002)

a) Spectrin
b) Laminin
c) Collagen
d) Elastin
Ans. A
168. Which of these is not a part of extracellular matrix:- (AIIMS MAY 2002)
a) Laminin
b) Fibronectin
c) Integrins
d) Collagen
Ans. A
169. Membrane fluidity is increased by: (AIIMS MAY 2005)

a) Stearic acid
b) Palmitic acid
c) Cholesterol
d) Linoleic acid
Ans. D
170. Eukaryotic plasma membrane is made up of all except: (AIIMS MAY 2009)
a) Carbohydrates
b) Triglycerides
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c) Lecithin
d) Cholesterol
Ans is B
171. Eukaryotic plasma membrane is made up of all except: (AIIMS MAY 2010)
a) Carbohydrates
b) Triglycerides
c) Lecithin
d) Cholesterol
Ans is B
T opic -Citric acid cycle

172.Which end product of citric acid cycle is used in detoxification of ammonia in brain
(AIIMS MAY 2001)
a) Oxaloacetate
b) Alphaketoglutarate
c) Succinate
d) Citrate
Ans. is B
173. Acetyl Co-A acts as a substrate for all the enzymes except: (AIIMS MAY 2003)
a) HMG-Co A synthetase
b) Malic enzyme.
c) Malonyl Co A synthetase.
d) Fatty acid synthetase.
Ans. B
174. First substrate of Kreb's cycle is (AIIMS MAY 2007)

a) Pyruvat
b) Glycine
C) HC1
d) Lipoprotein
Ans. is A
175. Which of the following substance binds to Co A Ah and condenses oxaloacetate to

inhibit the TCA cycle: (AIIMS NOV 2010)
a) Malonate
b) Arsenite
c) Fluoroacetate
d) Fumarate
Ans is C
176. Which of the following is not an intermediate of citric acid cycle? (AIIMS MA Y
2014)
a) Succinyl CoA
b) a-ketoglutarate
c) Citrate
d) AcetylCoA
Ans is D
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T opic -Collagen
177. The 40 nm gap in between the tropocollagen molecule in collagen which serve as
the site of bone formation is occupied by which of the following- (AIIMS NOV
2006)
a) Carbohydrate
b) Ligand moiety
c) Ca++
d) Fe+++
Ans is C
178. Collagen of which type is found in hyaline cartilage: (AIIMS NOV 2007)
a) Type I
b) Type II
c) Type III
d) Type IV
Ans is B
179. The 40 nm gap in between the tropocollagen molecule in collagen which serve as
the site of bone formation is occupied by which of the following- (AIIMS NOV
201 1)
a) Carbohydrate
b) Ligand moiety
c) Ca++ Fe
d) Fe++++
Ans is C
180. Long spacing collagen fibrils are found in? (AIIMS MAY 2014)
a) Cornea
b) Tympanic membrane
c) Diaphragm
d) Basement membrane
Ans is A
T opic -Ketone bodies
181. In a patient with starvation for 72 hrs which of the following would be seen:- (AIIMS
MAY 2002)
a) Increased glycogenolysis
b) Increased ketosis due to break down of fats
c) Degradation of proteins
d) Increased gluconeogenesis
Ans. B
182. Acetyl coA can be directly converted to all except (AIIMS MAY 2010)
a) Glucose
b) Fatty acids
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c) Cholesterol
d) Ketone bodies
Ans is A
183. Acetyl coA can be directly converted to all except (AIIMS MAY 201 1)
a) Glucose
b) Fatty acids
c) Cholesterol
d) Ketone bodies
Ans is A
184. ' Ketone bodies cannot be utilized by: (AIIMS MAY 2013)
a) Heart
b) Brain
c) Skeletal muscles
d) RBCs
Ans is D
185. A person with von Gierke's disease has ketosis. All are true for ketosis in this
patient except(AIIMS MAY 2013)
a) Lactic acidosis
b) Hypoglycemia
c) Fat mobilization is less
d) Hypertriglyceridemia
Ans is C
T opic -Trace elements
186. Biological role of metallothioneins is to sequester harmful metal ions. These bind
(AIIMS MAY 2004)
a) Cd++, Cu"""" & Zn**
b) Al***, Hg^ & NK,+
c) Pt++,As+~&PO4-
d) Fe+++, Na+&K+
Ans. A
187. Increased copper excretion in urine is seen in all except (AIIMS MAY 2007)
a) Primary sclerosing cholangitis
b) Wilson's disease
c) Primary biliary cirrhosis
d) Hepatocellular carcinoma
Ans. is D
188. Zinc is a cofactor for: (AIIMS NOV 2009)

a. Pyruvate dehydrogenase
b. Pyruvate decarboxylase
c. a-keto glutarate dehydrogenase
d. Alcohol dehydrogenase
Ans is D
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189. Which of the following is a non-essential metal/mineral? (AIIMS NOV 2010)

a) Sodium
b) Manganese
c) Iron
d) Lead
Ans is D
190. Biomineralization is seen in: (AIIMS MAY 2013)

a) Pinna
b) Molluscs shell
c) Hooves of Cattle
d) Nails
Ans is B
T opic -Genetic code
191. Amber codon refers to (AIIMS MAY 2001)

a) Mutant codon
b) Stop codon
c) Initiating codon
d) Codon for more than one amino acids
Ans. is B
192. Misexpression of which of the following homeobox genes alters the position of the
forelimbs during development (AIIMS MA Y 2004)
a) HOXA7
b) HOXB8
c) HOXC9
d) HOXD10
Ans. B
193. If a 4 nucleotide sequence code for an amino acid instead of 3, then theoretically
how many unique amino acids could be coded by such a system? (AIIMS NOV
201 1)
a) 16
b) 64
c) 128
d) 256
Ans. is D
194. Same aminoacid is coded by multiple codons d/t following: (AIIMS NOV 2011)
a) Degeneracy
b) Frame-shift mutation SI
c) Transcription
d) Mutation
Ans. is A
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T opic -Glycogen metaboloism
195. In muscle, phosphorylase b is inactivated by: (AIIMS NOV 2008)

a) cAMP
b) Ca ions
c) Glucose
d) A TP
Ans is D
196. Which of the following is not degraded by colonic flora? (AIIMS MAY 2011)
a) Pectin
b) Lignin
c) Starch
d) Glucose
Ans is B
197. A child was found to have hypoglycemia, hepatomegaly and accumulation of highly
branched glycogen called limit dextrins. He is likely to be suffering from (AIIMS
MAY 2015)
a) Cori's Disease
b) Von Gierke's Disease
c) Anderson's Disease
d) McArdle's Disease
Answer: A
198. Enzyme used for both glycogenesis and glycogenolysis is (AIIMS MAY 2015)
a) Glycogen synthase
b) Phosphoglucomutase
c) Glycogen transferase
d) Glucotransferase
Answer: B
T opic -Protein structure
199. The 3 dimensional shape of a protein is maintained mainly by: (AIIMS NOV 2003)
(a) Strong covalent interactions
(b) Interactions with other proteins
(c) Multiple weak interactions
(d) Interactions with prosthetic groups
Ans. is C
200. The primary structure of a protein refers to (AIIMS MAY 2004)

a) Linear structure and order of the amino acids present
b) Regular confirmational forms of a protein
c) Complete three-dimensional structure of the polypeptide units of a given protein
d) Subunit structure of the protein
Ans. A
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201. Which one of the following is not true for an a-helix (AIIMS MAY 2004)
a) It is one of the most important secondary structure
b) It has a net dipole moment
c) All hydrogen bonds are aligned in the same direction
d) Long stretches of left handed a-helices occur in proteins
Ans. is D
202. All of the following can determine the protein structure, Except: (AIIMS NOV 2008)
a) High performance liquid chromatography
b) Mass spectrometry
c) X-ray crystallography
d) NMR spectrometry
Ans is A
T opic -DNA Blot
203.Northern blot test is used for (AIIMS NOV 2001)

a. DNA analysis
b. RNA analysis
c. Analysis of proteins
d. Enzyme analysis
Ans. B
204. Western Blot detects? (AIIMS NOV 2009)

a. DNA
b. RNA
c. Protein
d. mRNA
Ans is C
205. Which of the following technique can be used for exact localization of a genetic
locus? (AIIMS MAY 2013)
a) Chromosome painting
b) Western blot
c) Comparative genomic hybridization
d) FISH
Ans D
T opic -Galactosemia
206.A child presents with hepatomegaly and bilateral lenticular opacities. Deficiency of
which of the following enzymes will not cause such features. (AIIMS NOV 2001)
a. Galactose-1-phosphate uridyl transferase
b. UDP-galactose-4-epimerase
c. Galactosekinase
d. Lactase
Ans. is‘D
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207. A newborn infant refuses breast milk since the 2nd day of birth, vomits on force-
feeding but accepts glucose-water, develops diarrhea on the third day , by 5th day
she is jaundiced with liver enlargement and eyes show signs of cataract. Urinary
reducing sugar was positive but blood glucose estimated by glucose oxidation
method was found low. The most likely cause is deficiency of: (AIIMS MAY 2003)
a. Galactose-1-phosphate uridyl transferase.
b. Beta galactosidase.
e. Glucose-6-phosphatase.
d. Galactokinase
Ans. A
208. The molecule, which is the intiator of cataract formation in the eye lens and whose
1-phosphate derivative is responsible for liver failure, is: (AIIMS MAY 2005)
a. Sorbitol
b. Mannitol
c. Inositol
d. Galacticol
Ans. D
T opic-Gluconeogenesis
209. During gluconeogensis reducing equivalents from mitochondria to cytosol are

transported by (AIIMS NOV 2002)
(a) Malate
(b) Aspartate
(c) Glutamate
(d) Oxaloacetate
Ans. A
210. Increased levels of alanine in serum after fasting suggest: (AIIMS NOV 201 1)
a) Increased release of alanine from muscle
b) Reduced amino acid utilisation for gluconeogenesis
c) Break in continuity of plasma membrane resulting in leakage of amino acids
d) Decreased uptake of alanine by liver
Ans is A
21 1. Which of the following enzymes is common for both glycogenolysis and

gluconeogenesis? (AIIMS MAY 2014)
a) Hexokinase
b) Glucose-6-phosphatase
c) Phosphoglucomutase
d) Glucokinase
Ans is B
T opic -ACID BASE
212. A buffer that is most effective at a pH of about 4.5 is; (AIIMS NOV 2003)
a) Acetate buffer.
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b) Bicarbonate buffer
c) Phosphate buffer
d) Tris buffer
Ans. is A
213. In a solution the concentration of hydrogen ion (H+) is 1 x 106 moles/litre. The pH
of the solution will be: (AIIMS MAY 2006)
a) Three
b) Six
c) Nine
d) Twelve
Ans. B
T opic -Alkaptonuria
214. A 40 yr old woman presents with progressive palmoplantar pigmentation. X-ray

spine shows calcification of intervertebral discs. On adding Benedict's reagent to
urine, it gives greenish brown precipitate and blue-black supernatant fluid. What is
the diagnosis? (AIIMS MAY 2010)
a) Phenylketonuria
b) Alkaptonuria
c) Tyrosinemia type-2
d) Argininosuccinic aciduria
Ans. is B
215. In Phenylketonuria the main aim of first line therapy is: (AIIMS NOV 2010)
a. Replacement of the defective enzyme
b. Replacement of the deficient product
c. Limiting the substrate for deficient enzyme
d. Giving the missing amino acid by diet
Ans is C
T opic -HMP Shunt
216. The cause of hyperuricemia and gout in glucose-6-phosphatase deficiency is :

(AIIMS NOV 2001)
a.. More formation of pentose
b. Decreased availability of glucose to tissues
c. Increased accumulation of sorbitol
d. Impaired degradation of free radicals
Ans. is A
217. NADPH is produced by: (AIIMS NOV 2003)

a) Glycolysis
b) Citric acid cycle
c) Hexose monophosphate shunt
d) Glycogenesis
Ans. is C
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T opic - Oxygen dissociation curve
218. True regarding conversion of deoxyhemoglobin to oxyhemoglobin is: (AIIMS MAY

2002)
a) Binding of O2 causes release of H+.
b) One mole of deoxyhemoglobin binds two moles of 2,3DPG
c) pH of blood has no affect on the binding of O2
d) Binding of O2 causes increased binding of 2,3,DPG
Ans. A
219. What is the most acute effect of smoking (AIIMS MAY 2005)
a) Shift of oxyhemoglobin curve to the right
b) Increased ciliary function
c) Decreased mucous production
d) Decreased incidence of post operative pneumonia
Ans. A
T opic -Prion
220. Defect in folding of the protein result in which of the following clinical disease:-
(AIIMS MAY 2002)
a) Kuru
b) Migraine
c) Hypothyroidism
d) Myopia
Ans. A
221. If cellular proteins do not fold into a specific conformation, their function are
affected. Certain disorders arise, if specific proteins are misfolded. Which of the
following disorders arises due to conformational isomerization - (AIIMS NOV 2005)
a) Familial fatal insomnia
b) Hepatitis delta
c) Pernicious anemia
d) Lesch-Nyhan syndrome
Ans. A
T opic -Apoptosis
222. Cytosolic Cytochrome C mediates (AIIMS NOV 2006)

a) Apoptosis
b) Electron transport
c) Krebs cycle
d) Glycolysis
Ans. is A
T opic -Bilirubin
223. The conjugation of bilirubin to glucuronic acid in the liver; (AIIMS NOV 2003)
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(a) Converts a hydrophilic compound to a hydrophobic one
(b) Converts a hydrophobic compound to a hydrophilic one
(c) Enables the bilirubin molecule to cross the cell membrane
(d) Is increased during neonatal jaundice
Ans. B
T opic -Ciliary structure
224. All are involved in fast axonal transport, except (AIIMS NOV 2007)
a) Kinesin
b) Dynein
c) Lysosomes
d) Neurofilaments
Ans D
T opic -Creatinine
225. Which of the following estimates blood creatinine level most accurately (AIIMS
NOV 2006)
a) Jaffe method
b) Kinetic jaffe method
c) T echnicon method
d) Enzyme assay
Ans. is A
T opic -Inheritance pattern

226. ABO blood group is an example for (AIIMS MAY 2015)
a) Autosomal dominant
b) Autosomal recessive
c) Co- dominance
d) X linked recessive
Answer: C
T opic -Purine metabolism
227. End product of purine metabolism in non-primate mammals is (AIIMS MAY 2007)
a) Uric acid
b) Ammonia
c) Urea
d) Allantoin
Ans. D
T opic -T oxin mechanism

228. Following modification occurs in Gs subunit which leads to watery diarrhea in
cholera (AIIMS MA Y 2011)
a) ADP ribosylation
b) A TP-ADP transfer
c) Phosphorylation
d) Dephosphorylation
Ans is A
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T opic -Urea cycle
229. An infant at 7months with history of vomiting and failure to thrive. Patient improved
with IV glucose. After one month returns with same complaints. On evaluation
found to have high urinary levels of glutamine and uracil. Which is the likely
enzyme defect (AIIMS MAY 2015)
a) CPS1
b) Ornithine transcarbamoylase
c) Arginase
d) Arginosuccinase lyase
Answer: B
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MD Entrance Coaching Notes for Biochemistry

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MD Entrance Coaching Notes for Biochemistry

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Dr.

Murali Bharadwaz Classes for MD Entrance Coaching Programe

AIIMS BIOCHEMISTRY NOTES

Major Topics Minor topics

1. vitamins 26. DNA Blot

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4. Which of the following combinations of biologically active molecules does vitamin A

7. Vitamin A is stored mainly as retinol esters in - (AIIMS NOV 2005)

8.Vitamin A intoxication causes injury to (AIIMS MAY 2007)

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9.Active form of vitamin D is (AIIMS MAY 2007)

10. Vitamin which is excreted in urine is : (AIIMS MAY 2007)

11. Vitamin K is required for (AIIMS NOV 2007)

12. Thiamine deficiency causes decreased energy production because(AIIMS MAY

14. Carboxylation of clotting factors by vitamin K is required to be biologically active.

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17.Thiamin requirement increases in excessive intake (AIIMS MAY 2009)

21. In chromatography mass movements of the substances in due to : (AIIMS MAY

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26. Nephelometry is based on the principle of (AIIMS NOV 2007)

27.Replacing alanine by which amino acid will increase UV absorbance of protein at

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28.Which amino acid migrates fastest on paper chromatography on methylcellulose

30. In CRP , the C stands for: (AIIMS NOV 2009)

31. Which statement is incorrect: (AIIMS NOV 2009)

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T opic -DNA replication

38. Xeroderma pigmentation is caused due to a group of closely related abnormalities in

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41.SYBR Green Dye is used for: (AIIMS MAY 2007)

42. Cation used in PCR is(AIIMS NOV 2007)

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47. CAP in LAC operon is (AIIMS NOV 2011)

49. Real time PCR is used for: (AIIMS MAY 2013)

50. Real time PCR is used for: (AIIMS NOV 2013)

51. Dietary triglycerides are transported by (AIIMS MAY 2001)

53. Cholesterol present in LDL : (AIIMS MAY 2003)

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55. Increased level of lipoprotein(a) predisposes to (AIIMS NOV 2006)

56. Triglycerides are maximum in (AIIMS MAY 2007)

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T opic -Amino acid

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69. At physiological pH, the carboxy-terminal of a peptide is (AIIMS NOV 2005)

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75.NO is synthesized by: (AIIMS MAY 2009)

T opic -DNA Structure

77. At the physiological pH the DNA molecules are : (AIIMS MA Y 2003)

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78. Rapid method of chromosome identification in intersex is (AIIMS MA Y 2007)

79. Y-chromosome is: (AIIMS MAY 2007)

80. Intron is not found in which DNA? (AIIMS NOV 2008)

82. Histone acetylation causes? (AIIMS MAY 201 1)

83. Triplex DNA is due to (AIIMS MAY 2011)

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85.Enzymes Ea,Eb and Ec are under allosteric control of S2 and PI and P2

88. Chymotrypsinogen is a (AIIMS NOV 2006)

89. Non-functional enzymes are all except: (AIIMS NOV 2008)

90.True about isoenzymes is: (AIIMS NOV 2011)

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92. Chymotrypsinogen is a: (AIIMS NOV 201 1)

93. Which of the following is a suicide enzyme? (AIIMS MAY 2013)