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  • Figure 2. The Restriction Map of The Plasmid PSF

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    Redha Alouaoui
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    The document contains a restriction map of the plasmid pSF. The map shows the locations of various restriction enzyme cut sites around the plasmid, including the 16S rRNA gene insert. It also contains figures showing a gel electrophoresis of a PCR product for the 16S rRNA gene from the SF strain and an unlabeled figure.

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    Figure PCR

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    The document contains a restriction map of the plasmid pSF. The map shows the locations of various restriction enzyme cut sites around the plasmid, including the 16S rRNA gene insert. It also contains figures showing a gel electrophoresis of a PCR product for the 16S rRNA gene from the SF strain and an unlabeled figure.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    7 views1 page

    Figure 2. The Restriction Map of The Plasmid PSF

    Uploaded by

    Redha Alouaoui
    The document contains a restriction map of the plasmid pSF. The map shows the locations of various restriction enzyme cut sites around the plasmid, including the 16S rRNA gene insert. It also contains figures showing a gel electrophoresis of a PCR product for the 16S rRNA gene from the SF strain and an unlabeled figure.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    A p aI ( 1 4 )

    A atI I ( 2 0 )
    Sp h I ( 2 6 )
    BstZI ( 3 1 )
    N o cI ( 3 7 )
    N o tI /BstZI ( 4 3 )
    SacI I ( 4 9 )
    N aeI ( 3 9 6 8 )
    1 T7
    (pGE M -T 3 0 1 5 p b)
    Ori f1 g ène 1 6 S
    X mn I ( 3 2 6 1 ) (1 5 0 5 pb)

    pSF-16S
    SacI ( 3 1 4 1 ) Amp R 4520 p b

    SP6
    lac Z H in d I I I ( 1 5 6 4 )
    Ori pUC S p eI ( 1 5 6 8 )
    N o tI /BstZI ( 1 5 8 1 )
    P stI ( 1 5 9 2 )
    N d eI ( 1 6 0 1 )
    S acI ( 1 6 1 3 )
    BstX I ( 1 6 2 2 )
    N silI ( 1 6 4 1 )
    SalI ( 1 6 3 4 )

    Figure 2. The restriction map of the plasmid pSF

    (a) (b) (c)

    Figure 3. (a): Marqueur de taille 1 Kb DNA Ladder; (b): PCR profile of the 16S rRNA gene
    of the SF strain;
    (c): ????????????????????????????

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