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Study of Soil Carbon Levels As A Result of Forest Management Techniques
Study of Soil Carbon Levels As A Result of Forest Management Techniques
Fall 2017
communities. This experiment was designed to measure how forest management techniques like
prescribed burns, thinning and harvesting would affect the amount of carbon stored in the soil. A
total of 6 areas were randomly sampled totally 18 samples per trip and placed into a muffle
furnace at roughly 500 degrees C for 48 hours. The results yielded probably no change in soil
Introduction
Carbon levels in soil are vital to the survival and prosperity not only of the ecosystem it is
present in but also for the overall health of the Earth. Soil carbon sequestration, microorganisms,
biodiversity, decomposition along with climate change are heavily effected and dependent on the
amount of carbon stored in the soil. Soil carbon levels can be influenced by changes in
environment due to various forest management techniques including prescribed burns and
harvesting.
Soil carbon storage (carbon sequestration) levels are rely on the activity and state of the
plants, animals and microorganisms that live in and depend on the soil. Evidence has shown that
forest management techniques have significantly increased regenerating forest’s soil carbon
levels (Dixon et al., 1994). In turn a healthy forest prospers and aids in the reduction of CO2
emissions via photosynthesis (Peng et al., 2008). This process of minimizing greenhouse gases is
more prevalent and beneficial in the more mid-latitude regions where temperature levels are
higher (Dixon et al., 1994). The abundance of carbon sequestration also aids in food productivity
and an increase in yield of various crops such as maize (10 to 20 kilograms per hectacre) or
wheat (20 to 40 kilograms per hectacre) (Lal 2004). Healthy forests also require the
Microorganisms play a key role in the overall health of the ecosystem. These organisms can
process the carbon in the soil increasing the efficiency of carbon use and decreasing the carbon
emission to the atmosphere (Allison et al., 2010). The rate of carbon consumption is related to
the temperature of the environment and can be a positive feedback loop process when regarding
climate change (Allison et al., 2010). Depending on the location, temperature also plays a key
role in the survival of specific microorganisms which in turn affect the carbon processing rate
(Carney et al., 2005). Presence of certain fungi and specific bacteria due to environmental
changes may also positively change the abundance of carbon present in the soil for a short period
of time (Busse et al., 2008). Some microorganisms may prohibit or inhibit certain plant species
from sprouting.
Plant health related to the amount of nutrients present in the soil. The global carbon levels are
highly altered based on the presence or absents of these soil bound organisms (Nielsen et al.,
2011). Certain fungi species can process and break down certain compounds like cellulose which
in turn helps increase decomposition rates of carbon (Cox et al., 2001; Hanson et al., 2008).
Animals that reside in the soil have also contributed to litter fragmentation and digestion,
transportation of microorganisms, and feeding microorganisms that reside in the ground (Swift et
al., 1979; Seastedt, 1984; Ingham et al., 1985; Wardle, 2002; Bardgett, 2005; Cole et al., 2006).
As a result biodiversity can have a positive impact on soil sequestration and preservation (Diaz et
al., 2009). In the end, decomposition and climate change are also dependent on the organisms
CO2 levels in the atmosphere as well as organic and inorganic litter decomposition are
affected by soil carbon levels. Mitigating climate change specifically relies on biodiversity that
aids in carbon sequestration, capture, and preservation (Diaz et al., 2009). Higher temperatures
would results in a higher decomposition rate causing more carbon to be released into the
atmosphere thus creating a positive feedback loop (Davidson et al., 2006). Soil carbon levels aid
in climate change and greenhouse gases thus should be explored more thoroughly.
The objective of this experiment is to measure the soil carbon levels in soil present in the
Southern New Jersey Pinelands and observe the effects of forest management techniques from
56.1° C, November 46.8° C, and December 37.2° C (“NCEI”). In this area the dominant forest is
the Pinelands with vegetation such as pond pine, loblolly pine, oaks, and maple trees.³ The soils
that dominate the sampled area are AtsA, DocB, HbmB, MakAt, and MumA where the most
prevalent type of soil is an entisol (“Forest Fire Research and Education”, “GALLOWAY
SERIES”). The tested areas were recently cut in the Spring/Summer of 2015 and was burned in
November 2016 (“Forest Fire Research and Education”). The Stockton University Forest Fire
Research and Education partnered up with the New Jersey Department of Environmental
Protections (NJDEP) to start the Stockton Forest Management Plan (SFMP) to perform
prescribed burns in order to protect and study the effects on the local ecosystems (“Forest Fire
Research and Education”). There were 6 areas sampled total (control unburned, control burned,
clearcut burned, clearcut unburned, thinned burned and thinned unburned) and 3 soil samples
were taken from each area, totaling 18 samples. Our group used a random number generator to
determine the amount of paces we would take into the forest. We would generate two numbers,
each for a different direction. When arriving at the location we would brush away and debris
located on the O layer and dig roughly 8 to 12 inches into the A layer using an auger. Samples
were collected in a metal tin and labeled. When returning to the lab the tins would be weighed
without the lid and placed into an incubator set at 500° C for 24 hours. After 24 hours the
samples were removed from the incubator and reweighed (without lid). The change in weight
would determine the soil moisture value. The freshly incubated sample was then crushed with a
pestle to break up and soil clumps or debris and separated into a smaller crucible. This crucible
was weighed before and after the soil addition. Again the samples were placed into an incubator
set at 500° C and incubated for 24 hours. After 24 hours the samples were reweighed and the
difference between the starting and ending weights determined the level of carbon in the soil. All
data was placed into a Microsoft Excel document and analyzed through ANOVA Single Factor
and an Error Bar Graph or SAS Enterprise for Wilcoxon-Mann Whitney and Kruskal-Wallis test.
Figure 2: Map of Stockton University with defined areas of study (Control, Clearcut, and burned areas) generated using ArcMap
GIS. Soil types also marked on map. Map source from Google Earth and soil types from NRCS Soil Survey.
Results
Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Clearcut Burned 9 39.17093 4.352325 4.658689
Clearcut Unburned 9 37.03426 4.114917 31.36099
Thin Burned 13 37.87464 2.913434 8.851602
Thin Unburned 10 41.91214 4.191214 10.94963
Control Burned 14 43.50843 3.107745 10.11911
Control Unburned 11 64.66508 5.878643 30.56195
ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 66.91739 5 13.38348 0.863365 0.511063 2.36827
Within Groups 930.0912 60 15.50152
Total 997.0086 65
Figure 3: Six way ANOVA Single Factor generated using data in Microsoft Excel.
5
Averave Carbon Levels (%)
0
Clearcut Burned Clearcut Thin Burned Thin Unburned Control Burned Control
Unburned Unburned
Figure 4: Mean Carbon level in soil from clearcut burned, clearcut unburned, thin burned, thin unburned, control burned, and
control unburned areas.. Error bars represent 1 standard error from the mean. No significant difference found at p = 0.05 level.
Comparisons were made using a Protected Least Squared Difference Test.
P= 0.511063, therefore I failed to reject the null hypothesis. Data are compatible with
statistical model. Probably, there is no significant difference in soil carbon levels between all six
Statistic 1082.0000
Normal Approximation
Z 0.9852
One-Sided Pr > Z 0.1623
Two-Sided Pr > |Z| 0.3245
t Approximation
One-Sided Pr > Z 0.1641
Two-Sided Pr > |Z| 0.3282
Z includes a continuity correction of
0.5.
Table 1: Wilcoxon-Mann Whitney test results generated through SAS Enterprise comparing burned vs. unburned. P=0.3245.
P=0.3245, therefore I failed to reject the null hypothesis. Data are compatible with statistical
model. Probably the soil carbon % between burned and unburned areas are the same.
Kruskal-Wallis Test
Chi-Square 7.9001
DF 5
model. Probably the soil carbon % between clearcut burned/unburned, thin burned/unburned and
Discussion
After gathering data the first test that was performed was a 6 way ANOVA single factor, the
results show that among any of the areas studied that there were no significant differences of
carbon soil percent. To further the analysis, a Wilcoxon-Mann Whitney test between the burned
and unburned areas was conducted and showed that there is also no significant difference of soil
carbon levels. Typically there will be no changes in soil carbon levels as a result of forest
fires (Johnson 1992). In this experiment harvesting took place over one year earlier than the
burned/unburned, thin burned/unburned, and control burned/unburned and the results still state
that there is no significant difference of carbon levels between each area. Changes of soil carbon
levels and soil nutrients have been measured to be much lower when 15 to 45 cm [5.9 to 17.7
inches] deep in the soil (Pennock et al., 1997). Our measured levels were around 8 to 12 inches,
which falls into the category of less significant soil nutrient levels.
This experiment experienced many shortcomings including limited time frame of 3 months,
sampling too deep within the soil, and miscommunication between group members. All of these
factors added up to the results that were obtained and only allowed limited statistical tests to be
ran.
Conclusion
Overall comparing each area studied as well as being burned or unburned, there was probably
no significant changes of soil carbon levels present in the soil samples. This shows that forest
management techniques of harvesting, prescribed burns, and thinning probably had no effect. In
the future it can be recommended to increase the sampling timeframe from just three months to
approximately a year, or constant data collection throughout various semesters and comparing
the results from years previous. Sampling should also not be taken deep within the A horizon but
In the future a different approach to statistical analysis should take place. For the case of this
class, it was required to run an ANOVA Single Factor assuming the data is normally distributed
and performing further analysis based on those results. Under normal circumstances in SAS
Enterprise a full Proc Univariate can be ran to test for population distribution normality. If
sample was not normally distributed a transformation can be applied then re-evaluated. Once the
variances, which would determine which T-Test (equal or unequal) should be interpreted. Once
all assumptions for the ANOVA are met (normally distributed population, random sampling and
homogeneity of the variances), it can be ran and interpreted. When significant differences are
shown a Tukey Test, Student-Newman-Keuls (SNK) Test, Least Significant Difference (LSD)
Test, etc. may be ran to pinpoint where the differences lie. If the ANOVA assumptions cannot be
met then either a Levene’s or Bartlett’s test should be performed to test the homogeneity of the
variances. In this experiment no Proc Univariate was performed and it was assumed that the
population was normally distributed and the variances were assumed to be equal.
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