F A C U L T Y O F M E D I C I N E

Course Code: BMS 201

Lab 11
• Identify RBCs, platelets and WBCs in blood films (blood smear)
• Identify TEM photos for platelets and WBCs.
Ø Number: 150,000-
400,000/mm3
Ø Size small 2-4 μm
fragment
Ø Origin:- Platelets arise as
fragmentation of giant
polyploid
megakaryocyte
( Bone marrow cells).
Ø It contains numerous
specific granules of
platelets.
Ø Mature megakaryocytes
have numerous
invaginations of plasma
membrane
demarcation membranes.
 HYALOMERE

GRANUOLMERE
 Granulomere
Central granular region

Hyalomere
Peripheral clear region
Thrombus
Classification of leucocytes according to presence of
specific granules and the shape of their nuclei
WBCs in blood film (blood smear)
Identify the WBCs in blood film (blood smear)?
Neutrophils
 Neutrophil (TEM)
1. Segmented or multi-
lobed nucleus
2. Electron dense large
granules [ lysosomes
nonspecific granules]
3. Electron lucent small
granules [ specific
granules]
 Eosinophils
It is increased in parasitic worms & in
allergic conditions
 Eosinophil (TEM)
1. Bi-lobed nucleus
2. Large ovoid
specific granules
with electron
dense core
Eosinophil (TEM)
 Basophils
It is increased in allergic conditions and anaphylaxis
 Basophils TEM
1. Bi-lobed nucleus
2. Large spherical
homogenous
electron dense
specific granules
Monocytes Lymphocytes
 Case study
9 years-old boy presented to Pediatrics clinic with nose bleeding(epistaxis) and
complained of increased skin discoloration over (bruising )over the last month. The
pediatrician asked for CBC blood test and the results were as follows:
• WBC: 6500/μL (4000-11,000/μL)
• Hemoglobin: 14.5 g/dL (13.5-17.5 g/dL)
• Platelets: 44,000/μL (150,000- 400,000/μL)

A-Is there something abnormal in these lab

results?

B-If there is an abnormality, could it be correlated

to clinical findings? Clarify
1

Ø Identify the cell in the front image

Ø Mention types.
Ø How you can differentiate between
types
2

Ø Identify the cell in the front image

Ø Mention their number.
Ø Mention 2 characters
3

The arrow shows:

1. Azurophilic granules
2. Lysosomes
3. Melanin granules
4. Specific granules

Identify the cell

4

Ø Identify the cell in the front

image
Ø Mention their number.
Ø Mention 2 characters
5

Ø Identify the cell in the front image

Ø Mention their number.
Ø Mention 2 characters
6
7
Time for Students’ Questions
Textbook guide

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