1

Analysis Report
Name：201113_TRAINING 1
Notes:
Sample analysis rules: Ct≤ 35 is positive; Ct> 38 is negative; others are
undeterminated.
Protocol:
Lid：105 Volume：30
1 Temperature55.0℃,0:15:0
2 Temperature95.0℃,0:0:30
3 Temperature94.0℃,0:0:10
4 Temperature54.0℃,0:0:15
5 Temperature72.0℃,0:0:20
6 Goto 3, 4x
7 Temperature94.0℃,0:0:10
8 Temperature58.0℃,0:1:0Plate Read
9 Goto 7, 39x
10 End
Amplification

Experimental results:

# Sample Target Type Dye Ct Call

A1 irma wati ORF1ab UNK FAM / Negative
A1 irma wati N Gene UNK HEX / Negative
A1 irma wati E Gene UNK ROX / Negative
A1 irma wati IC UNK Cy5 25.19 Positive
A2 imanoviana ORF1ab UNK FAM / Negative
A2 imanoviana N Gene UNK HEX / Negative
A2 imanoviana E Gene UNK ROX / Negative
A2 imanoviana IC UNK Cy5 25.26 Positive
 2

A3 marganing ty ORF1ab UNK FAM / Negative

as ratnasari
A3 marganing ty N Gene UNK HEX / Negative
as ratnasari
A3 marganing ty E Gene UNK ROX / Negative
as ratnasari
A3 marganing ty IC UNK Cy5 23.71 Positive
as ratnasari
A4 ujang s ORF1ab UNK FAM / Negative
uhendar
A4 ujang s N Gene UNK HEX / Negative
uhendar
A4 ujang s E Gene UNK ROX / Negative
uhendar
A4 ujang s IC UNK Cy5 24.30 Positive
uhendar
A5 bella ri ORF1ab UNK FAM / Negative
smawanti
A5 bella ri N Gene UNK HEX / Negative
smawanti
A5 bella ri E Gene UNK ROX / Negative
smawanti
A5 bella ri IC UNK Cy5 24.51 Positive
smawanti
A6 mega pur ORF1ab UNK FAM / Negative
nama sari
A6 mega pur N Gene UNK HEX / Negative
nama sari
A6 mega pur E Gene UNK ROX / Negative
nama sari
A6 mega pur IC UNK Cy5 24.46 Positive
nama sari
A7 arya suryana ORF1ab UNK FAM 34.06 Positive
A7 arya suryana N Gene UNK HEX 32.28 Positive
A7 arya suryana E Gene UNK ROX / Negative
A7 arya suryana IC UNK Cy5 25.43 Positive
A8 rahmat wijaya ORF1ab UNK FAM / Negative
A8 rahmat wijaya N Gene UNK HEX / Negative
A8 rahmat wijaya E Gene UNK ROX / Negative
A8 rahmat wijaya IC UNK Cy5 24.35 Positive
B1 arief ali ORF1ab UNK FAM / Negative
B1 arief ali N Gene UNK HEX / Negative
 3

B1 arief ali E Gene UNK ROX / Negative

B1 arief ali IC UNK Cy5 26.28 Positive
B2 asasas ORF1ab UNK FAM / Negative
burahman
B2 asasas N Gene UNK HEX / Negative
burahman
B2 asasas E Gene UNK ROX / Negative
burahman
B2 asasas IC UNK Cy5 23.30 Positive
burahman
B3 acep yudi w ORF1ab UNK FAM / Negative
B3 acep yudi w N Gene UNK HEX / Negative
B3 acep yudi w E Gene UNK ROX / Negative
B3 acep yudi w IC UNK Cy5 24.16 Positive
B4 elis trisnawati ORF1ab UNK FAM 23.17 Positive
B4 elis trisnawati N Gene UNK HEX 21.57 Positive
B4 elis trisnawati E Gene UNK ROX 23.5 Positive
B4 elis trisnawati IC UNK Cy5 22.57 Positive
B5 santi lestari ORF1ab UNK FAM / Negative
B5 santi lestari N Gene UNK HEX / Negative
B5 santi lestari E Gene UNK ROX / Negative
B5 santi lestari IC UNK Cy5 25.53 Positive
B6 kania fitriani ORF1ab UNK FAM 33.63 Positive
B6 kania fitriani N Gene UNK HEX 31.17 Positive
B6 kania fitriani E Gene UNK ROX 30.80 Positive
B6 kania fitriani IC UNK Cy5 25 Positive
B7 muhamad syah ORF1ab UNK FAM 31.82 Positive
ril abdullah
B7 muhamad syah N Gene UNK HEX 28.78 Positive
ril abdullah
B7 muhamad syah E Gene UNK ROX 29.71 Positive
ril abdullah
B7 muhamad syah IC UNK Cy5 25.48 Positive
ril abdullah
B8 suryana ORF1ab UNK FAM / Negative
B8 suryana N Gene UNK HEX / Negative
B8 suryana E Gene UNK ROX / Negative
B8 suryana IC UNK Cy5 23.84 Positive
C1 ghisca ORF1ab UNK FAM 35.14 ?
amanda a
C1 ghisca N Gene UNK HEX / Negative
amanda a
 4

C1 ghisca E Gene UNK ROX / Negative

amanda a
C1 ghisca IC UNK Cy5 23.18 Positive
amanda a
C2 diana pu ORF1ab UNK FAM 33.27 Positive
spitasari
C2 diana pu N Gene UNK HEX 30.80 Positive
spitasari
C2 diana pu E Gene UNK ROX 35.06 ?
spitasari
C2 diana pu IC UNK Cy5 24.02 Positive
spitasari
C3 nada raudatul ORF1ab UNK FAM / Negative
C3 nada raudatul N Gene UNK HEX / Negative
C3 nada raudatul E Gene UNK ROX / Negative
C3 nada raudatul IC UNK Cy5 25.26 Positive
C4 niswatul ORF1ab UNK FAM / Negative
waisiqoh
C4 niswatul N Gene UNK HEX / Negative
waisiqoh
C4 niswatul E Gene UNK ROX / Negative
waisiqoh
C4 niswatul IC UNK Cy5 23.85 Positive
waisiqoh
C5 irma hidayati ORF1ab UNK FAM 33.71 Positive
C5 irma hidayati N Gene UNK HEX 32.32 Positive
C5 irma hidayati E Gene UNK ROX / Negative
C5 irma hidayati IC UNK Cy5 24.59 Positive
C6 risania enjelina ORF1ab UNK FAM / Negative
C6 risania enjelina N Gene UNK HEX / Negative
C6 risania enjelina E Gene UNK ROX / Negative
C6 risania enjelina IC UNK Cy5 24.05 Positive
C7 ria sobari ORF1ab UNK FAM / Negative
ah karomah
C7 ria sobari N Gene UNK HEX / Negative
ah karomah
C7 ria sobari E Gene UNK ROX / Negative
ah karomah
C7 ria sobari IC UNK Cy5 24.23 Positive
ah karomah
C8 kaysa qonita h ORF1ab UNK FAM / Negative
C8 kaysa qonita h N Gene UNK HEX 32.56 Positive
 5

C8 kaysa qonita h E Gene UNK ROX / Negative

C8 kaysa qonita h IC UNK Cy5 25.09 Positive
D1 zunairah ORF1ab UNK FAM / Negative
tsabita
D1 zunairah N Gene UNK HEX / Negative
tsabita
D1 zunairah E Gene UNK ROX / Negative
tsabita
D1 zunairah IC UNK Cy5 26.03 Positive
tsabita
D2 rania helmi ORF1ab UNK FAM / Negative
D2 rania helmi N Gene UNK HEX / Negative
D2 rania helmi E Gene UNK ROX / Negative
D2 rania helmi IC UNK Cy5 26.80 Positive
D3 aliya zakiyah z ORF1ab UNK FAM / Negative
D3 aliya zakiyah z N Gene UNK HEX / Negative
D3 aliya zakiyah z E Gene UNK ROX / Negative
D3 aliya zakiyah z IC UNK Cy5 26.03 Positive
D4 khodijah ORF1ab UNK FAM 36.29 ?
D4 khodijah N Gene UNK HEX / Negative
D4 khodijah E Gene UNK ROX / Negative
D4 khodijah IC UNK Cy5 24.23 Positive
D5 qurrota syania ORF1ab UNK FAM / Negative
D5 qurrota syania N Gene UNK HEX / Negative
D5 qurrota syania E Gene UNK ROX / Negative
D5 qurrota syania IC UNK Cy5 24.57 Positive
D6 ratna ke ORF1ab UNK FAM / Negative
suma dewi
D6 ratna ke N Gene UNK HEX / Negative
suma dewi
D6 ratna ke E Gene UNK ROX / Negative
suma dewi
D6 ratna ke IC UNK Cy5 24.62 Positive
suma dewi
D7 rafa jihan d ORF1ab UNK FAM / Negative
D7 rafa jihan d N Gene UNK HEX / Negative
D7 rafa jihan d E Gene UNK ROX / Negative
D7 rafa jihan d IC UNK Cy5 23.21 Positive
D8 rina ORF1ab UNK FAM / Negative
D8 rina N Gene UNK HEX / Negative
D8 rina E Gene UNK ROX / Negative
D8 rina IC UNK Cy5 25.07 Positive
 6

E1 NEGATIVE ORF1ab NTC FAM / Negative

E1 NEGATIVE N Gene NTC HEX / Negative
E1 NEGATIVE E Gene NTC ROX / Negative
E1 NEGATIVE IC NTC Cy5 / Negative
E2 NTC ORF1ab NTC FAM / Negative
E2 NTC N Gene NTC HEX / Negative
E2 NTC E Gene NTC ROX / Negative
E2 NTC IC NTC Cy5 / Negative
E3 POSITIVE ORF1ab POS FAM 21.46 Positive
E3 POSITIVE N Gene POS HEX 21.39 Positive
E3 POSITIVE E Gene POS ROX 22.87 Positive
E3 POSITIVE IC POS Cy5 22.27 Positive