1

Analysis Report
Name：D:\neoplex nyoba
Notes:
Sample analysis rules: Ct≤ 35 is positive; Ct> 38 is negative; others are undeterminated.
Protocol:
Lid：105 Volume：20
1 Temperature50.0℃,0:30:0
2 Temperature95.0℃,0:15:0
3 Temperature95.0℃,0:0:15
4 Temperature60.0℃,0:1:0Plate Read
5 Goto 3, 39x
6 End
Amplification

QC results:

- Definition Amount #
1 Amp fluorescence exceed limit 0
2 Ct Std Dev of replicate wells >0.5 0
3 Ct of NTC <35 0
4 No Ct in unknown sample -
8 Outlier in replicate wells with P<0.05 0
11 Positive control Ct≥35 0
12 Negative control Ct≤38 0

Experimental results:

# Sample Target Type Dye Ct Results

A1 neg rdrp NTC FAM / Negative
A1 neg n NTC JOE / Negative

2020-12-16 13:03:04 Wed

 2

A1 neg ic NTC Cy5 / Negative

B1 13 rdrp UNK FAM 27.89 Positive
B1 13 n UNK JOE 26.78 Positive
B1 13 ic UNK Cy5 28.62 Positive
C1 14 rdrp UNK FAM 22.68 Positive
C1 14 n UNK JOE 20.93 Positive
C1 14 ic UNK Cy5 27.45 Positive
D1 15 rdrp UNK FAM / Negative
D1 15 n UNK JOE / Negative
D1 15 ic UNK Cy5 29.78 Positive
E1 16 rdrp UNK FAM / Negative
E1 16 n UNK JOE / Negative
E1 16 ic UNK Cy5 27.81 Positive
F1 17 rdrp UNK FAM / Negative
F1 17 n UNK JOE / Negative
F1 17 ic UNK Cy5 31.16 Positive
G1 18 rdrp UNK FAM / Negative
G1 18 n UNK JOE / Negative
G1 18 ic UNK Cy5 29.86 Positive
H1 pos rdrp POS FAM 32.63 Positive
H1 pos n POS JOE 33.67 Positive
H1 pos ic POS Cy5 33.73 Positive

2020-12-16 13:03:04 Wed