51 me: Tamayo, Hannah Beata A pepram/Venr/Section: seins: 4 Date Performed, 2021 Group Number: Date Submitted: Nov. 7,202 Experiment No. 8 HDL CHOLESTEROL OBJECTIVES 1, To determine the HDL concentration fr ' 2, To compute for the LDL concentration. eee 3. To know the clinical significance of the test. INTRODUCTION Cholesterol is a lipidic molecule, in; in which it circulates in form of pseu proteins constituting lipoproteins, Lipids are transported in the plasma to various body tissues by lipoproteins. Lipoproteins are particles with triglycerides and cholesterol esters in their core and phospholipids and free cholesterol near the surface. Lipoproteins also contain one or more specific proteins, apoproteins, located on the surface of the particle These lipoproteins vary quantitatively and qualitatively in their lipidic and proteic composition, including structural but such a functional difference to them. The most used Classification is that which is based on their difference in density. This explains the name of High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), Very Low Density Lipoprotein (VLDL) and the existence of many intermediate fractions which correspond to all the stages of the lipidic metabolism. HDL (lipoproteins of high density) contain approximately 50% of lipids including 20% of cholesterol. The HDL molecule plays an integral role in femoving cellular cholesterol and thus cellular purification. Many epidemiologic studies confirmed the anti-atherogen function of this fraction leading to the concept of “good cholesterol’. Cholesterol HDL represents consequently an element of evaluation of the atherogenesis risk when there is an imbalance of the tatios of cholesterol total/cholesterol HDL or cholesterol LDL/cholesterol HDL. soluble in aqueous medium as plasma, ido-emulsion; association of lipids and METHOD: PRECIPITATION. PHOSPHOTUNGSTIC ACID (ELITECH) PRINCIPLE - , ___ Chylomicrons, Very Low Density Lipoproteins (VLDL) and Low ee Lipoproteins (LDL) of serum are precipitated by phosphotungstic acid an Magnesium ions. After centrifugation, high density lipoproteins (HDL) are in ue supematant. Cholesterol included in this phase, Is measured by an enzymatic MethodCOMPUTATIONS QUESTIONS ; seutod 1. What is lipid profile? in the The droutt oF Speci rat molecuites Fermact UDid conical, S Measured by the pi ope medsurg Quon a a teluding ign ms Cts jero| QS a p' 8 ooh file $ Used A Frees mo nick af Cardiovateulat dongcton? Such Uh as her digedso, heart attack , aid Stoke in bath chuldten afd adults. 2. What are lipoproteins? Different tate the AyPas of LPPs. Lpopmtens dre _dicinchve porh le Made Up oF pat sige las ones? in ae Pg re us, IF mokcales, “ite rote fr, teu poi dn. ‘ha thy de aC ot tpl ek pe ouvert Cia ae HOF ‘ayn “dome Ih to body'cals, whi ra PN iu ANA. 1S in Chy VLOL is. produced. el 4 f, us faa ang more me si Ir df tan eine 6 smut Sg cause a aah fF eholyskro} aang than tien 1 SO i LD ES a Te? (Us an ea i Han 10, Phoing Ht Ho, wo tall yer nd ie ti pircla @ HDL tt tf char joe f ii on pugley fron cite te Ives As a sulk, HL Toffen Fen kro *§ Ore Mt oN55 3. Whatis the clinical significance of the giest? me ae tet 'S RqWind bo monthor a eye ese choles eu bens os thainjain cane Cholast eral (ured - cas ni tons Ss ie the aseccinet iovas cular ws conta thy bry ond mind Fush ou to saber uate oe Stroke , heart ai a 1Q Person mi Se, and athe osclonsic» A “4 ren deliyere Baause chuletyenl and tig ave water “insoluble, aay mgt be sites. in COmpeHA th, pe ap fe ie tom ‘paces Hate a central Core vn Bist) ne Cholest Surrount Mee. Fara! Shape sie Se ia hey Vegi pias tn the lise ome ee Were a ts ead 05-4 Doral eat. the upid Ig Uued tr Owes wee Bee iva Tlar disectes in st children ( Confinugtion of 42) vrata lig med hat cam yale b als ore not al ds cr eonkd di ye li a Us i) en ret SN ee et ry aft moe en ee ie “ate i panes ay ies Tu rte ee re ys “ho pa ae ay mse te Wa eee dese ea ie Bs Mae Lakak Pane rie eetName: _[Omayo,iannah Beata A pis: —_femis-38 Group No. 56 Date Submitted: _ Nev. 1,203) Rating: LABORATORY REFORT Title: woe ewes et —_ CATEGORY EXCELLENT ‘GOOD FAIR WEEDS (5 pts) (pts) (1 pt) IMPROVEMENT Le (Opt) Data Entry jaucecures co ‘One required datum is | Several data are ‘Ailrequired data , missing i number, date $ messing ae performed ete) are present. Drawings? —_| Clear, accurate Drawings/diagrams | Drawings/éiagrams are | Needed Diagrams Grawings/ diagrams | are included and are _| included but lack drawings/diagrams are included and labeled accurately. important labels. are missing. make the experiment easier to understand, They are labeled neatly and acourately. and Analysis ‘Observation | Observations and data are complete and totally accurate. Many observations made, may not be well organized or complete, ‘Some observations made, but lacking in significant detail Tables or other data presentations are incomplete. No observation was made. Questions The questions fo be answered during the report are clearly identified and stated. The questions to be answered during the report are identified, but are stated ina somewhat unclear manner. The questions to be answered during the report are partially identified, and are stated in a somewhat unclear manner. The questions to be answered during the report are erroneous oF irrelevant. Conclusion Presents a logical explanation for findings. Describes any errors found in the experiment. Presents some explanation for findings and address few questions. Makes some connections to Presents an illogical explanation for findings and does not address any of the questions suggested in the ‘No conclusion was included in the report. Discusses what was | what is being studied | introduction. Contains learned in the in class, some explanation of experiment and why lab was makes connections performed, but does to the topics being not make connections studied in class. to what is being studied in class. Participation | Used time well in the | Used time pretty well. | Did the experiments Participation was room and focused attention on the experiment. Stayed focused on the experiment mast of the | interested. Focus was time. lost on several but did not appear very | minimal or stude was hostile abou participating. occasions. Satisfactory Rating: 85% Comments: _