36 Name: Tamayo. Honnah Poatne® A Date Performed: et 2 202 Program/Year/Section:#smis3A Date Submitted: OC? .31,, 20. Group Number: Experiment No. 7 WHITE BLOOD CELL COUNT OBJECTIVES 1. To practice performing and calculating a manual white cell count. 2. To know the precautions observed when doing the test. INTRODUCTION . Leukocytes are white blood cells that defend the body against infective microorganisms and remove debris, including dead or injured host cells. There are about 4,000 to 11,000 leukocytes per cubic millimeter of blood. Leukocytes are primarily in the body tissues but are transported in the circulation Principle: Whole blood is mixed with a weak acid solution to dilute the blood and hemolyze the red blood cells. MATERIALS / EQUIPMENT / REAGENTS WBC pipet WBC diluting fluid Neubauer hemocytometer 70% alcohol thick coverslip EDTA anticoagulated blood microscope Unopette counter ; Petri dish sucking tube filter paper’ ¥ gauze/tissue PROCEDURE 1. Draw the blood up to the 0.5 mark of a WBC pipet. 2. Remove the blood from the outside of the pipet with a clean gauze. 3. Holding the pipet almost vertical, place the tip into the fluid. Draw the WBC. diluting fluid up to 14»mark, while gently rotating the pipet to ensure a proper amount of mixing. . . Firmly cover the tip of the pipet with the index finger and detach the sucking tube attached from the other end. . Shake pipet for 3 minutes, either by hand or by mechanical shaker. Place the index finger over the top of the pipet to control the flow of blood. - Discard the first 4 or 5 drops of the mixture. Charge counting chamber. Allow to stand for 1 minute. x enonjective (LPO), count white blood calls in the four | 9. Using the low power ob) corner squares. count only those that touch the left unting cells, a ) double lines, disregarding th, Note: For consistency in col ting chambors will ide lines, in count upper outsi I i that touch the right and lower outside margin. Calculation: WBC counted x 10 x 20 WBC in thousand/mm? = r where 10 = depth correction factor (constant) 20 = dilution factor (variable) 4 = area correction factor (constant) NORMAL VALUES Conventional Units SI Units Adult 4,000-11,000/mm> 4,0-11.0 x 107L Newborn 10,000-30,000/mm* 10.0-30.0 x 10°%L 1 year old 6,000-17,000/mm? 6.0-17.0 x 10% DRAWINGS OBBERYATION i i @ while blyod cei) Count is dt ' From fag wine sau L every lor etna dua Corner squares squares Uting tN. 9 Toled tumble of areas 4s be civ d Inky lo Little jo vlocd sample is dilute Upaly a: ve)oedh gy coat k's Bl clynb! k's ECaid) , wach iesanes, shen ait n + Lyi, ( Vommon ly Kino wh Os waa. shale blow cuts Whe ysing a lood cel38 RESULTS / CALCULATION eno - What are the factors that affect the WBC count? Door Heed smear Prieta, Sng sig grprcguent te ste Cellreop gnihon and di ChnoPon Ue ‘slafistreal elrots are the, most typed Coure oF paneer my hand colinis “gp {00 or 200.@lls due bo the Limited Sample ste \ \ jh crue § Kyul cenul, roa tray be Mots geval or Foils Pato ah Conk a ted ihe rh pra Cav beh mn Ye aSp locume; ‘0 Granuloas agglu! unary reat by Surpaa, et te ‘niatektne woh 2. What are the conditions in which the WBC count is decreased or increased beyon: 12 y gemma 1 WBC cqutl ic a%Ocidled with those eonahians elacing cancun coused bych inothgrapy Wealments) ae ee: Cis: fea inant} Le oman s ons Ne, a) 1 radiation, aoe met meh te eae Ore ‘ortiet a mn jepioencie, (ver damage oars e Thea ups ‘on es other hand, Those Fe Fall Soh ihe ns CON couse he High: vika leche nas Fons mmanon Vind Sing, Dhysuall oF vapors trots, “bun Nite SySveM digas, temaval othe spon rare. bee ty, teakonse Land ‘ana 3. In ree count, tet be done when WBC is eer high? uliOph evese reatment eg: m 4c ith oma “high oie LS Sates Mp hoe oma ches i Dunn Feat i) is presse Lego Rte nes te tne ang 9. ait ener bcd Ws pury lee culls as rapidly as pus puepo ‘iste reaye? The quantity CONCLUSION 1s in {so known OS LeuhogJes Ore WiMume Culs FOUN IA the YL Ua pide estan end RSIS Toy oe eer Taken foe bays area ‘yen tN vo inrecton) pesictana, on) play a purchon n Mlammahbn ang all gic es poses ae ayannty oF white (egy calls! an a sample OF Q bleed iS tolgled Uh ah agar a tee re cuba ecaatl, Hhcl} ui aid doctors if ie gage Serge ea be delecb hy 4 oF Wert St ae eniyy ind Tosa eae39 Name: font enc Beahice A: Date Submitted: ¢l -3), 304 PIYIS: pts-J0 Rating: Group No. LABORATORY REPORT . Experiment No. 7 _ Title: White Blo CATEGORY | EXCELLENT GOOD FAIR NEEDS (5 pts) (3 pts) (1 pt) IMPROVEMENT (0 pt) Data Entry All required data ‘One required datum | Several data are missing. | Alll required data are (name, group is missing, missing number, date performed ete) are present. I Drawings? | Clear, accurate Drawingsidiagrams — | Drawings/alagrams are Diagrams drawings! diagrams | are included and are_| included but lack important are included and labeled accurately. labels. missing. make the experiment easier to understand. They are labeled neatly and accuratel ‘Observation | Observations and | Many abservations | Some observations made, | No observation was and Analysis | data are complete made, may not be but lacking in significant made. and totally accurate. | well organized or detail. Tables or other data | complete. presentations are | incomplete. ‘Questions | The questions lobe | The questions tobe | The questions tobe The questions to be answered during the | answered during the | answered during the report | answered during the report are clearly report are identified, are partially identified, and | report are erroneous or identified and stated. | but are statedina | are stated ina somewhat _| irelevant somewhat unclear | unclear manner. manner. Conclusion Presents a logical Presents some Presents an illogical No conclusion was explanation for ‘explanation for ‘explanation for findings and | included in the report. findings. Describes _| findings and address_| does not address any of any errors found in few questions. Makes | the questions suggested in the experiment. some connections to | the introduction. Contains Discusses what was | what is being studied | some explanation of why learned in the in class. lab was performed, but experiment and does not make connections makes connections to to what is being studied in the topics being class. studied in class. Participation | Used time well in the | Used time pretty well. | Did the experiments but did | Participation was room and focused Stayed focused on Not appear very interested. | minimal or student was attention on the the experiment most | Focus was lost on several | hostile about ‘experiment, of the time. occasions. participating, Satisfactory Rating: 85% Comments: