40 Date Performed: ott 29,204 Ni A lame: Tanays Hanyah Beatrice Date Submitted: «t 3) 2021 Program/Year/Section: Bsyils- 38 Group Number: Experiment No. 8 RED BLOOD CELL COUNT OBJECTIVES 1. To practice performing and calculating red cell count. 2. To know the precautions to observe when doing the test. INTRODUCTION Erythrocytes have several principal functions namely 1) transport of oxygen to the tissues, b) transport of carbon dioxide to the lungs, and 3) maintain normal blood pH through a series of intracellular buffers. They are approximately 5 million erythrocytes per cubic millimetre of blood. They are produced in the red bone marrow and found in the ribs, sternum, skull, vertebrae, and bones of the hands, feet, and pelvis. Numerous nutrients are needed for normal cell formation, including iron, vitamin Biz, folic acid, and pyridoxine. The red blood cell (RBC) count is a count of the number of circulating RBCs in a cubic milliliter of peripheral venous blood. When the value is decreased by more than 10% of the expected normal value, the patient is said to be anemic. RBC counts above normal can be physiologically introduced if the body requires greater oxygen-carrying capacity. Diseases that produce chronic anoxia also provoke this physiologic increase in RBCs. A specimen containing erythrocytes is diluted with a premeasured volume of isotonic saline diluting fluid that will not lyse erythrocytes. A diluted specimen is added to a hemocytometer for manual enumeration of erythrocytes. This manual can be used in special circumstances (e.g. body fluids). Principle: Whole blood is diluted with an isotonic diluting fluid to facilitate counting and prevent lysis of the red blood cells. MATERIALS / EQUIPMENT / REAGENTS RBC pipet RBC diluting fluid Neubauer hemocytometer 70% alcohol thick coverslip EDTA anticoagulated blood microscope gauze/tissue counter sucking tubej RBC pipet. | tine pipet with a clean gare, | to the fluid. Draw the Ra he pipet tO ENSUE a prog d detach the suey PROCEDURE So 4. Draw the blood up to ie ee a 2. Remove the blood from the ol nl 3. Holding the pipet almost vertical, place the ip i ‘ diluting uid up to 101 mark, while gently amount of mixing. oe | Firmly cover the tip of the pipet with the index finger 2! the other en aT mutes, either by hand or by mechanical ence r over the top of the pipet to contral the flow o blood, 5 drops of the mixture. ; ber. Allow to stand for 3 minutes. 0), place the large center square with: B tube attache’ Shake pipet for 3 mit Place the index finge! Discard the first 4 or Charge counting cham Using the low power objective (LP' tertiary squares, in the middle of the field vision. objective (HPO) and count the red blot 40. Change to high power a four comers and central portion of the tertiary squares. od cells int ©ernow t touch the left a ing cells, count only those that disregarding tho Note: For consistency in counti chambers with double lines, upper outside lines, in counting that touch the right and lower outside margin. Calculation: RBC in millionimm? = RBC counted x 10 x 200 x 5 where 10 = depth correction factor (constant) 200 dilution factor (variable) 5 = area correction factor (constant) Short-cut formula if dilution factor is 200: RBC counted x 10,000 = RBC counted + 4 zeroes RBC in million/mm? NORMAL VALUES Conventional Units SI Units Females fens 3,600,000-5,600,000/mm? 3.0-5.6 x 10% Newborn 4,200,000-6,000,000/mm? 42-60 x 10% 5,000,000-6,500, 000/mm? a 85 10" .0-6.5 x 1 year old 3 ,500,000-5, 100, 000-5, 100,000/mm* oa 3.5-5.1 x 10!42 DRAWINGS OBSERVATION. ee . , or the total num er oF Squares fy We tallied For RAC count enc! “square of contra) Square ts ufos contain ny if 3. In REC count what should ne done when the RBC. is extreme ren? Whed the ABC IS extemely high ‘in Counting, therodeutic phi ee fy dan 1plo80 yeh 0, confOiter yy vent sac ony r rescriphon oF hydroxyreg Fok down the Preduchon oF ed ‘i key rg Frat” E RES In cur : punt dow not rat us | Lp monvtored D ensurg ne Re 4. What are the precautions to be observed wh i | ‘The precukans tube obconed when aunty Ne BBe oe” | Counting chamber na pipelte should be glean and dry, ¢) ‘ it » Finger hy and vicking lah yt ubhiotl cna St be Sterile, blood shoud ly dome aut fo pipette, no. hie bufftful, % PFeken} cloHing oF blogg ne efi ismrken gl fey au laa hag 70nd shuld be properly muxeld ‘ith sitclad aul up ty ol eo atseted Aen Nd yo Oks ny Ut Ra Fore Cin Cal Tot of b game zag counting Mould be prom lepF bRghlCONCLUSION A red flood cél) count iS q blwd test performed to detemine the pumber oF RACs inthe body . Ages include, hemaglobin, which ntanspotts ‘Oy en ty the body "Ne lsh le aueial. the amount oF ‘Se oh doled ty ihe aio ends ay She quantihy OF Hcg ur body have to Furchon | OUr Hs Sues ware oxygen Measuring BCS can help ‘tog anemig, & Condihen in which the pees Stes oe enough heal th gd cols, RAC count nay als used to, help pes other catdihions \hat arpeat ted blood @ils, be as Hidndy ems, hype Of white Moodl! Gelh cancer, & problems With bone what45 Name: _Tomayo ah Yedice Date Submitted: ct. 3/, tty __ PIVIS: Rating: Group No. a LABORATORY REPORT Experiment No. oe Title: Rec Blac Coll Count — CATEGORY | EXCELLENT GOOD FAIR NEEDS (5 pts) pts) (1 pt) IMPROVEMENT (0 pt) Data Entry All required data ‘One required datum | Several data are missing. | All required data are (name, group is missing. missing. number, date performed etc) are _ present. Drawings? | Clear, accurate Drawingsidiagrams | Drawingsidiagrams are | Needed Diagrams —_| drawings! diagrams | are included and are _| included but lack important. | drawingsidiagrams are are included and labeled accurately. | labels. missing. make the experiment easier to understand. They are labeled neatly and accurately. ‘Observation | Observations and | Many observations | Some observations made, | No observation was and Analysis | data are complete | made, may not be —_| but lacking in significant | | made and totally accurate. well organized or detail. Tables or other data complete. presentations are incomplete. Questions ‘The questions to be ‘The questions to be. ‘The questions to be ‘The questions to be answered during the | answered during the | answered during the report | answered during the report are clearly report are identified, are partially identified, and | report are erroneous or identified and stated. but are stated in a are stated in a somewhat irrelevant. somewhat unclear | unclear manner. manner. Conclusion | Presents a logical Presents some Presents an illogical No conclusion was explanation for explanation for explanation for findings and | included in the report. findings. Describes _| findings and address _| does not address any of any errors found in | few questions. Makes | the questions suggested in the experiment some connections to | the introduction. Contains ;cusses what was | whatis being studied | some explanation of why leaned in the | in class. lab was performed, but ‘experiment and does not make connections makes connections to to what is being studied in the topics being class. studied in class. Participation | Used time well in the | Used time prelly well, | Did the experiments but did | Participation was room and focused | Stayed focused on _| not appear very interested. | minimal or student was attention on the the experiment most | Focus was lost on several | hostile about ‘experiment, of the time. ‘occasions. participating. Satisfactory Rating: 85% Comments: