Discussion

Electrical stimulation and manual stimulation were performed for each Renilla. In all four

groups the result shows manual stimulation having a longer bioluminescence time compared to

the electrical stimulation. Although manual stimulation was longer than electrical, data collected

showed electrical stimulation creating more pattern than the manual.

Wave patterns and stimulation for each stimulation was identical depending on the renilla,

some had greater time period of stimulation and no wave pattern, displaying only point of light

(or bioluminescence) at point of contact with the nodes, and others had longer period of

stimulation with strong wave pattern all over the renilla, while few renillas didn’t have no period

of stimulation at all and no sign of bioluminescence.

There were many factors in the disparity of results collected by each group, such factors

includes; the nature of the renilla (some renilla had longer period of bioluminescence no matter

no matter which group tested it, and how long they were tested they maintain a result within the

same range). Some other anomalies where noticed, the student expected the renilla to lit up at

every time it was stimulated but in some instances it was the case, they sometime lit up other

time they were unresponsive. Variation for each test was observed, in each stimulation on

different renillas had differences on their pattern, although the mean period overall shows

manual stimulation being higher than electrical stimulation, in some instance electrical period

shorter, so the result didn’t follow the group hypotheses of the bioluminescence expected.

The effects bioluminescence on renilla remains to be learned.

Conclusion

It was for the first team had dealt with bioluminescence with a living aquatic subject to

produce light, the fact that renilla can produce light through is fascinating. It leaves to wonder

what types of chemical matter interact with contact to produce light throughout the renillas’

body. And also to question the molecular structure that facilitate the emission of light. In

conclusion bioluminescence in renilla varied independently of each individual, but the overall

mean total of all the data put manual stimulation higher than its counterpart.

References

Farré, M., Asperger, D., & Kantiani, L. (2008, January 3). Assessment of the acute toxicity of

triclosan and methyl triclosan in wastewater based on the bioluminescence inhibition of

Vibrio fischeri. Annual Review of Bio Chemistry. Retrieved July 13, 2009.

doi:10.1007/‌s00216-007-1779-9

Woo, J., & von Arnim, A. (2008, January). Mutational optimization of the coelenterazine-

dependent luciferase from Renilla.Plant Methods, 4, 1-10. Retrieved July 22, 2009,

doi:10.1186/1746-4811-4-23
Zhao, H., Doyle, T., Wong, R., Cao, Y., Stevenson, D., Piwnica-Worms, D., et al. (2004,

January). Characterization of coelenterazine Analogs for measurements of renilla

luciferase activity in live cells and living animals. Molecular Imaging, 3(1), 43-54.

Retrieved July 13, 2009, from Academic Search Complete database.

Lehninger, A. L. (1982). Bioenergetics and Metabolism. In Principles of Biochemistry (p. 388).

Worth Publishers, Inc.

Loening, A., Wu, A., & Gambhir, S. (2007, August). Red-shifted renilla reniformis luciferase

variants for imaging in living subjects. Nature Methods, 4(8), 641-643. Retrieved July 14,

2009, doi:10.1038/nmeth1070