LEMBAR PERHITUNGAN REAGEN

A. Perhitungan Basis
Basis volume = 285 ml
ρ saat T = 30℃ = 0,995 gr/ml
Basis massa = ρ x v = 0,995 𝑥 285 𝑚𝑙 = 283,575 𝑔𝑟𝑎𝑚

B. Kebutuhan Tiap Variabel

 Variabel 1 ( nutrisi = 5%w, pH = 7, suhu = 30°C )
Suhu = 30°C
pH =7
Media = %v x v basis = 18% x 285 ml = 51, 3ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 5% x 285 ml = 14,25 ml
 Variabel 2 (nutrisi = 7%w, pH = 7, suhu = 30°C )
Suhu = 30oC
pH =7
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
 Variabel 3 ( nutrisi = 9%w, pH = 7, suhu = 30°C )
Suhu = 30°C
pH =7
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 9% x 285 ml = 25,65 ml
 Variabel 4 ( Nutrisi = 7%, pH = 4, Suhu = 30oC )
Suhu = 30oC
pH =4
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
 Variabel 5 ( Nutrisi = 7%, pH = 7, Suhu = 30oC )
Suhu = 30oC
pH =7
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
 Variabel 6 ( Nutrisi = 7%, pH = 9, Suhu = 30oC )
Suhu = 30oC
pH =9
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
 Variabel 7 ( Nutrisi = 7%, pH = 7, Suhu = 17oC )
Suhu = 17oC
pH =7
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
 Variabel 8 ( Nutrisi = 7%, pH = 7, Suhu = 30oC )
Suhu = 30oC
pH =7
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
 Variabel 9 ( Nutrisi = 7%, pH = 7, Suhu = 37oC )
Suhu = 37oC
pH =7
Media = %v x v basis = 18% x 285 ml = 51,3 ml
Aquadest = %v x v basis = 82% x 285 ml = 233,7 ml
Starter = %v x v basis = 12% x 285 ml = 34,2 ml
Nutrisi = %v x v basis = 7% x 285 ml = 19,95 ml
C. Total Kebutuhan Reagen
 Media = 51,3 ml x 9
= 461,7 ml
 Starter = 34,2 ml x 9
= 307,8 ml
 Nutrisi = 19,95 ml x 7 + 14,25 ml +25,65 ml
= 179,55 ml
 Aquadest = 233,7 ml x 9
= 2.103,3 ml