isoterma de ligacao

0.9

0.8

0.7
theta (% sitios ocupados

0.6

0.5

0.4

0.3

0.2

0.1

0
0 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009 0.01
concentracao de ligante total (mol/l)
 is related to the fraction of mutated 0
ession: A(A) = 0.40 el87H.
ein homology/model building I-
0~

^
10-
100 80 60 40 20 0
Em geral, um grau de identidade maior que 30 % indica
f the structures of related proteins can
ino acid sequence changes that have
Sequence Identity (0/)
(Perutz et al., 1965). Previous work
Fig. 1. Size of common
es has shown that mutations, insertions estrutura similar
cores as a function of protein homology. If two
proteins of length n1 and n2 have c residues in the common core, the
anges in three-dimensional structure fractions of each sequence in the common core are c/n1 and c/n2. We plot
1978; Lesk and Chothia, 1980, 1982, these values, connected by a bar,- against the residue identity of the core
(see Table II).
a and Lesk, 1982, 1984; Read et al.,
tematic comparison of structures from
ies. This shows that the extent of the
y related to the extent of the sequence
re we used the atomic coordinates of
hese structures have been determined oi
£
._

OA) and refined. The errors in their 0

0A (see references given in Table I).
ight different protein families and pro-
._

o
ous structures. a
V
L-

o
ores and the variable regions of hom- C

a.
0
ous proteins can be divided into those
ral fold of the polypeptide chains is 0
0
re it is quite different. In comparing
l to separate the parts that have similar
e folds differ. We did this using the
cedure: (i) the main-chain atoms of
ry structure - helices or two adjacent 100 80 60 40 20 0
individually superposed; and (ii) each Percent residue identity
tended to include additional atoms at
as continued as long as the deviations
oms in the last residue included were Clothia
backbone atoms of the common cores of 32 pairs ofEMBO
and
Fig. 2. The relation Lesk
of residue (1986)
identity J. 5: 823
and the r.m.s. deviation of the
homologous proteins
procedure defined the segments that (see Table I).
England 823