List of Reviewer’s Comments and Author’s Response

Manuscript ID: [MS 40-21]

R1 = ref4/ Reviewer 1 (Ex: R1-1 = comment no.1 of Reviewer 1 and so on …)

R2 = ref2/ Reviewer 2 (Ex: R2-1 = comment no.1 of Reviewer 2 and so on …)

No Question or Suggestion Answer or Response

.
Response to Reviewer 1
R1 Abstract Thank you for the suggestion, the referred word is changed
-1 Change word “activities” from “activities” to “activity”.

R1 Introduction Thank you for the suggested references namely Awad, et

-2 Update by adding some inserted al., 2014 and Hamed et al., 2015; both references are added
references to the introduction.

In reference:
Awad HM, El-Enshasy HA, Hanapi SZ, Hamed ER and
Rosidi B. 2014. A new chitinase-producer strain
Streptomyces glauciniger WICC-A03: isolation and
identification as a biocontrol agent for plants
phytopathogenic fungi. Nat Prod Res, 28(24): 2273-2277.

Hamed ER, Awad HM, Ghazi EA, El-Gamal NG and

Shehata HS. 2015. Trichoderma asperellum isolated from
salinity soil using rice straw waste as biocontrol agent for
cowpea plant pathogens. J Appl Pharm Sci, 5(2): 091-098.
R1 Methodology Thank you for the suggestion, we add the detail of media
-3 Mention the Source of this and chemical source. Moreover, we also add the detail of
medium (Company name, City, tools which used in this experiment.
Country) same comment for all
chemicals)
Mention the source of the media
through the whole text

R1 Methodology Thank you, we changed the word “media” to “medium”, as

-4 media : You should write medium suggested, in the whole text.
instead of the media through the
whole text ( Medium is the single
and the plural is media)

R1 Methodology Sorry about the miswords there, we changed the words

-5 collected from “collected from” to “obtained from”. Thank you for the
suggestion.

R1 Methodology Thank you about the suggestion, for avoiding any

-6 1 000 mL (delete space) misconception, we changed it to “1 L”.
No Question or Suggestion Answer or Response
.
R1 Methodology Thank you for reminding, we described the capacity and
-7 flask capacity and type type of the flask.

In text: 125 mL Erlenmeyer flask (Pyrex 4450-125)

R1 Methodology Thank you, we mention the details of the room temperature

-8 room temperature (mention the used on this study.
degree of temp.)
In text: room temperature (25 oC)

R1 Methodology Thank you for the suggestion, we decided to use preposition

-9 Add “A” “The”

In text: “The 20 mL inoculum …”

R1 Methodology Thank you for the question, we immediately tested the
-10 Mention which is method you enzyme activity.
used
In text:
… and the supernatant is immediately tested for its enzyme
activity.

R1 Methodology Thank you for the question, we add the composition detail
-11 arsenomolybdate reagent of that reagent.
(Mention its compostion)
In text:
The arsenomolybdate reagent was made of 25 g of
ammonium molybdate (Sigma-Aldrich, Germany) dissolved
in 450 mL distilled water, 21 mL of concentrated H2SO4 (JT
Baker, United States), and 3 g of Na2HAsO4.7H2O (Sigma-
Aldrich, Germany) dissolved in 25 mL of H2O, mix then
place in an incubator (Heraeus B5042, Germany) at 37 oC
for 24 to 48 hours.
RHS as Assoc Editor
24 h to 48 h
RHS corrected based on SI

R1 Methodology Thank you for the suggestion, as the Somogyi-Nelson assay

-12 Summarize the above paragraph. already described on above paragraph. We summarize and
shorten the explanation of standard curve assay.

In text:
The standard N-acetyl-glucosamine curves were prepared
by testing several N-acetyl-glucosamine concentrations (10,
20, 30, 40, 50, and 60 mg L-1) following exactly Somogyi-
Nelson assay which written above.

RHS as Assoc Editor (10, 20, 30, 40, 50, and 60) mg L-1
No Question or Suggestion Answer or Response
.
RHS corrected based on SI rule

R1 Methodology Thank you, we change the paragraph to prevent to repetition

-13 Then the mouth of the tube was
covered with aluminum foil
( repeated many times)
Summarise this detailes.
words.
In text:
The enzyme activity was stopped by heating it in boiling
water for 15 min before it was mixed with the reagent.
Furthermore, the supernatant obtained was taken as much as
1 mL to be tested by Somogyi-Nelson assay. The solution
was diluted with distilled water to a final volume of 10 mL
and read the absorbance at λmax with a phosphate buffer
blank which was treated the same as the sample.

R1 Methodology Thank you, we followed the suggested change.

-14 Is done
Rewrite carefully. In text:
The measurement of enzyme activity was done by
converting the absorbance value to the concentration of N-
acetyl-D-Glucosamine (Yang et al., 2016) and calculating it
following this equation (1):

R1 Methodology Thank you, we added the suggested title on methodology.

-15 Add this title
Determination of the optimum pH,
temperature and incubation time

R1 Methodology Thank you, we changed the sentences.

-16 …is carried… In text:
The procedure is that each test The determination of the optimum pH of chitinase was
tube is provided carried out with a variation of pH 6; 6.5; 7.0; 7.5; 8; and 8.5.
Each test tube was provided with 1 mL chitin 2 % (w/v) in a
phosphate buffer with a pH variation of 6; 6.5; 7.0; 7.5; 8;
and 8.5.
RHS as Assoc Editor
RHS corrected based on SI rule

R1 Methodology Thank you, we changed “molybdenum blue complex” to

-17 molybdenum blue complex “molybdenum blue complexes”.

R1 Methodology Thank you, we added the suggested title on methodology.

-18 Add title
Effect of Ca2+ ions

R1 Methodology Thank you, we changed the word “namely” to “as follows”

-19 Change wording “namely”
R1 Results Thank you for the suggestion, we double checked and
No Question or Suggestion Answer or Response
.
-20 Figure caption changed all the figure caption.

R1 Results Thank you, we followed the suggested change.

-21 Title
The Role of Calcium ions on In text:
Chitinase Enzyme Activity The Role of Calcium ions on Chitinase Enzyme Activity

R1 Discussion and Conclusion
-22 change the style of citation
R1 Discussion and Conclusion
-23 the enzyme to bind to the substrate
to form an Enzyme-Substrate
complex- re-write carefully
Thank you for the suggestion, we changed it as suggested.
In text:
… He et al. (2020), …
… Nguyen and Nguyen (2020), …
Thank you for the suggestion, we rewrite the sentences
carefully.
In text:
Calcium ion acts as an activator for enzymes that work to
hydrolyze a macromolecule. The Ca2+ ions change the
conformation and orientation of the active site of the
enzyme, also increase the activeness of the enzyme to bind
to the substrate to form an Enzyme-Substrate complex.

R1 Discussion and Conclusion Thank you, we separate the Results and Discussion part,
-24 We ask the authors to flow the following the journal format style
journal format style through all the
text The JJBS -Instruction to authors states: "the results and
discussion part can also be described as separate, if
RHS comments appropriate. The Results and Discussion sections can
include subheadings, and when appropriate, both sections
can be combined".
In my opinion, the provisions above state: the results and
discussion section should not be separated. Or the results
and the discussion section can be combined.
Please see: combined section in the URL;

http://jjbs.hu.edu.jo/files/vol14/n2/Paper%20Number
%2014.pdf

http://jjbs.hu.edu.jo/files/vol14/n2/Paper%20Number
%2015.pdf

http://jjbs.hu.edu.jo/files/vol14/n2/Paper%20Number
%2021.pdf

R1 Discussion and Conclusion Thank you for the suggestion, we did the proofreading and
-25 We ask the authors to send the corrected the grammatical mistakes.
manuscript for simple
proofreading to double check the
No Question or Suggestion Answer or Response
.
some grammatical mistakes

R1 References Thank you, we did it as your suggestion.

-26 Double check for the references in
the text is found in the ref. list and
vice versa

R1 References Thank you for the suggestion, we changed it following

-27 All the references are not on the journal format style.
journal format style

RHS Comments Thanks a lot for reviewer suggestion. At the time, this
manuscript submitted to JJBS:
(i) we have not yet received a decision from JJBS regarding
the rule of reference format, specifically "work with a
foreign language (non-English)". We recommend using
APA or MLA Style. Considerations in APA or MLA
include the original title (non-English), making it easier
for netizens to track, for example, on Goggle.
(ii) we also include DOI to make it easier for netizens to
track, e.g. on Google.
The two items above, we have now adjusted after the
decision from EIC JJBS.
Another explanation asked by reviewers is the use of bold in
several references. We do "bold" according to EIC
instructions, namely in the manuscripts sourced from JJBS.

Response to Reviewer 2
R2 Abstract Thank you, we changed it as your suggestion.
-1 glycosidic
R2 Abstract Thank you for the suggestion, we changed the word.
-2 7.5 pH RHS as Assoc Editor, disagree about the reviewer’s
sugestion
R2 Abstract Thank you, we changed it as your suggestion.
-3 activity
R2 Abstract Thank you, we changed the word.
-4 on the
R2 Abstract Thank you, we changed it as your suggestion.
-5 profitable chitinase We also adjust the keywords.
R2 Introduction Thank you, we changed the word.
-6 which is
R2 Introduction Thank you, we changed it as your suggestion.
-7 increasing
No Question or Suggestion Answer or Response
.
R2 Materials and Method Thank you, we changed the word.
-8 which was
R2 Materials and Method Thank you, we changed the word format to Italic.
-9 Italic
R2 Materials and Method Thank you, we changed it as your suggestion.
-10 petri dish
R2 Materials and Method Sorry for the confusion, we changed the misword.
-11 ?
In text:
… put 10 mL each into petri dish …
R2 Materials and Method Thank you, we changed it as your suggestion.
-12 cultures
R2 Materials and Method Thank you, we added the word.
-13 The
R2 Materials and Method Thank you, we changed the word.
-14 disappeared
R2 Results Thank you, we changed it as your suggestion.
-15 trials
R2 Results Thank you, we changed the word.
-16 shown
R2 Results Thank you, we changed it as your suggestion.
-17 cause
R2 Discussion Thank you, we added the word.
-18 know about the
R2 Discussion Sorry for the confusion, we changed the misword.
-19 side or site?
In text: … active site …
R2 Discussion Thank you, we changed it as your suggestion.
-20 enzymes bind
R2 References Thank you, we double check all the reference format.
-21 Kindly, check format.
R2 References Thank you for the suggestion, we removed this reference
-22 Please remove this reference
This reference contains the same
information which is presented at
first in the article of Jahromi ST
and Barzkar N. 2018. Marine
No Question or Suggestion Answer or Response
.
bacterial chitinase as sources of
energy, eco-friendly agent, and
industrial biocatalyst. Int. J. Biol.
Macromol. 120: 2147–2154.
DOI:10.1016/j.ijbiomac.2018.09.0
83
R2 References Thank you, we double check all the reference format.
-22 ?
R2 References Thank you, we double check all the reference format.
-23 ?
RHS Comments Corresponding author changes the title of the figure by
using every word in capital letters (according to the
reviewer's recommendation).
In my opinion, the JJBS provisions use lowercase letters in
the figure and table titles, except at the beginning of the
letters of the title.
Please see this URL:

http://jjbs.hu.edu.jo/files/vol14/n2/Paper%20Number
%2015.pdf