Study of Gamete
Development in_
: Mouse (Mammal)
|: To study and identify the spfges of gamete development in mouse (mammal) i.e., T-S. of
testis and L.S. of ovary thfough permanent slide.
UIREMENTS
Permanent slide of TS. of testis and L.S. of ovary, microscope.
/EDURE
Fix the permanent slide under the microscope. First observe it under the low power and then
Mer high power.
ERVATION
Of Testis
‘The testis of a mouse (mammal) is covered by a thick fibrous tissue called tunica albuginea.
‘The testis consists of numerous seminiferous tubules embedded in the interstitial tissue.
Various types of germinal cells are present from outside towards lumen in the following sequence.
Spermatogonia -> Spermatocytes -> Spermatids —> Spermatozoa — Sperms.
Between the germinal cells, pyramid shaped cells called sertoli cells are present.
‘Scanned wih CamScanner
|
stilComprehensive Laboratory Manual in
66 ate Presene
i
ed in sertoli cells,
large number of spermatozoa with their heads embedded in ser
lumen of seminiferous tubule.
‘The interstitial tissm also contain leydigs cells, mina mal texhormone estoy,
Viecorat
Pettoneum —
Interstitial
calls
Fig. 11.1. A.A Part of transverse section of test
* Sectional view of a part of seminiferous
tis of mouse (mammal).
is tubule (enlarged)
V.S. Of Ovary
1. Amouse
rae. Solid structure bounded by germinal ‘pithelium followed by a thick ayergt
Abrous tissue, the tunica abuginia,
Cortex
Corpus luteum,
Ruptured follcte
Fig. 11.2. A section of ovary of mouse (mammal).
‘Scanned wih CemScanner4 ‘The ovary consists of outer cortex and inner medulla.
"4 The medulla contains many rounded or oval bodies called ovarian or Graafian follicles at various
stages of development.