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Three Commonly used Sterilization Techniques in Tissue Culture
13th Apr 2021
Three Commonly used Sterilization Techniques in Tissue Culture
In this article, you will learn about three different most commonly used
sterilizing techniques, their principles, and what purpose they serve. So,
let’s begin!
1. Autoclaving
It’s a physical method of killing microorganisms including bacteria,
viruses, and spores. This device uses steam under regulated pressure to
achieve sterilization of the materials put inside the vessel. But how does
it work?
Principle of Autoclaving
It works on the principle of moist heat sterilization. The boiling
temperature of the water is 100 °C under normalHi! How can I help you?
atmospheric pressure.
But, when the atmospheric pressure is increased, the boiling
temperature of the water also increases, thus helping achieve a higher
temperature for sterilization. So, if the steam pressure inside the vessel is
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The high pressure inside the vessel also facilitates the rapid penetration
of heat into deeper parts of the material. The moisture in steam causes a
change in the protein structure of microorganisms that leads to loss of
function and activity of microbes.
Use of Autoclave
It can be used to sterilize your glass apparatus, solid or liquid media,
distilled water, normal saline, discarded cultures, and contaminated
media.
If you don’t have a bigger autoclave you can buy a reasonable size
pressure cooker to sterilize your materials. You can refer to the plant cell
technology Youtube video for more information on the pressure cookers
and which one you should use.
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Source: https://amlinstruments.co.uk/advice/what-is-a-for...
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3. Filter Sterilization
Some substances like plant growth regulators or vitamins are heat-labile
and can not be sterilized using any kind of heat sterilization method. In
these cases, filter sterilization is the best solution! In this method,
microbes are not killed but removed or filtered out from the materials.
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1. Pore size
2. The electric charge of the filter
3. Nature of the fluid being filtered
So, these are the three most commonly used sterilization techniques
used in tissue culture labs worldwide to sterilize the materials. In our
upcoming articles, you will learn in-depth about all these techniques.
And, if there is any topic that you want to learn more about, then you can
write your suggestions at anjali@plantcelltechnology.com.
Happy culturing!
REFERENCES
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