the test card.

If the reaction pad has a blue color before applying saliva sample, do not
se A. Agents which enhance color de velopment
3. Saturate the reactive pad with saliva from collection cup or by applying saliva directly to
D-ALC-S12 One Step Saliva Alcohol Test the pad. (It usually takes 6-8 seconds to be saturated.) Start fimer immediately after saliva Strong oxidizers
application. B. Agents which inhibit color development
Read result at two (2) minutes. Compare the color of the reaction pad with the chart on the
strip to determine the relative blood alcohol level.
.Reducing agents: AscorbC acid, Tannic acid, Pyrogallol, Mercaptans and tosylates,
Oxalic acid, Uric Acid.
The One Step Saliva Alcohol Test is a rapid, highly scnsitive mecthod to detect the presesce of Bilirubin
alcohol in saliva and provide an approximation of relative blood alcohol coneentration. Ldopa
his test provIdes a preliminary screen only. A more specific altermate chemical method must L-methyldopa
be Uuscd in order to obtain a confimed analytical result. linical consideration and Methampyrone
professional judgment should be applied to any test Ncreen result, particularly 'when
preliminary positive screens are indicated.
The One Step Saliva Alcohol Test
may be qualitattvely verified by usng a test solution
SU NNARY preparedby adding 5 drops of 80 proofdistilled spirits to 3 oz. (I cup) of water. This solution
so procccoo reaction on the pad.Thecolor eaction wtth alcohol in saliva is
soTiewhat slower and les intense than with alcohol in an aqueus solution.
Two-thirds of all adults drnk alcohol. The blood alcohol concentration at which a person
becomes mpaired 1s variable dependent upon the individual. Each individual has specific
parameters that afect the level ot impairment such as size, weight, eating habits and alcohol
tolerance. Inappropriate consumption of alcohol can be a contributing factor to many
accidents, injuries, and medical conditions.
Volpicellim,
1.
Joseph R.. MD, Ph.D.: Aleohol Dependence
Drugnosis, ClinIcal Aspeets and BiopsychosoCial Catuses.. Substance Abuse Library,
PRINCINH University of Pennsylvania. 1997
Jones, A. W. Inter-and intra indvviduai varratiorns In the saiva/biood alcohol ratto during
INTERPRETATION OF RESU1.IS_ ethanol metabolism m man.. Cln. Chem. 25. 1394-1398. 1979.
I S ecstablished that the concentration of alcohol in sal1va is comparable to that of MaCall, L.E.L., Whitng. B., Moere, M.K. and Goidberg A.. Correlalson of thanol
c stnp With a reaction pad Positive: The One Step Saliva Alcohol Test will concentrations in blood and salva., Clin.Se.. S6. 283-286. 1979.
attached at the tip. On c o n t a c t with solton produce a color change in the presence o
nre P w i la rapidy
T n e pad employs tun saliva alcohol. The color w1ll range from light blue color at
colors depending on the concentration of aleohol reerit solid-phase 0.02% relative blood alcohol
concentration to a dark bluc color ncar 0.30% relative blood alcohol concentration. Color pads
chemistry which uses a highly specific enzyme react
are provided within this range to allow an approximation of relative blood alcohol
concentration. Ihe test may produce colors that
appcar to
be between adjacent color pads.
REGENIS The
E Catalog number
NOTE: One_StepthanSaliva Alcohol Test is very sensitive presence of alcohol. A blue
to the
Consuit instructioms o LOT BRch code
color that is
lighter the
Tetramethvlbenzid1ne presence of alcohol in saliva but less than 0.02% relative blood alcohol. g Positlve to lhe VD
in vutro dagnostic meticaí devnce te by
Alcoho! Oxidase(F-C11.3.13) Do o
Mamu tact:irer
Peroxidase (EC 1,J1.,7)
Otlher additives Nepative:When the Oneresult
ntctpreted as a negative Stepindicating
Saliva Alcolhul Test shows
that alcohol has not no color
been change
detected.
this should be

Invalid: If the color pad has a blue color belore applying saliva sample, do not use the lest.
PRECAUTIONS
NOTE: A result where the outer cdges of the color pad produces a slight color but the
TheOne Step Saliva Alcohol Test is a Visually interpreted test where color matching is u d De Tefpeated to ensure
complete saturation
used to provide an approximalion of relat1ve blood alcoho! concentration. Test materials of the pad with San I ne teer
saliva The test IS not reusable.
that have
Onc been cxposcd to saliva should be trcaiod as potentially infectious. Do not use the
Step Saliva Alcohol Test after the expiraiion dale marked on thc foil package.
gulations. LIMITAIONS
1. Failure to
wait 15 minutes afer placing food, drink, or other materials
in the
(1ncluding smoking)
mouth before running the test can produce erroneous results due to pO8SIDIC
contamination of the saliva by interfering substances.
The One Step Saliva Alcohol Test is to be stored at 2-27C (36-80°F) in its sealcad tol 2 h e One Step Saliva Alcohol Test is highly sensitive to the presence of alcohol. Alcohol
product is refrigerated, the One Sep Saliva Aicohol Test must be brough! to room a ar are Sometimes detecled by the One Step Saliva Alcohol Test. Alcohol
temperature prior to opening the pouch. household products such as disnfeo _ conol 1s a component in

cleaners.
manyy
It thc
presenoc ol alcohol vapors 1s test dh
suspecied, the test
is suspected. a gldss
should be perlomed in an area Knowt u
n e c ol
be iroe ol vapors.
MAHRIALS PROVJDED 3. ingestion or general use of over-the-counter med1cations and products containing alcoho
Can produce positive results.
Individually foil pouched test cards
Packagr isert
collectuon cups PRTORNAN ECJARACTRISTK'S
The delection limit on the One Step Saliva Alcohol Test
MALRIALA RtY IRID B NOI PROVUND approximate relative blood alcohol level. The cutoff level ofisthefrom
Onc 0.02% to 0.30
Step Saliva for
Alcohol
esi can any ased on local tegulatiorns and laws. Test results can be compared to reference
Tinmer ie vels wiln colar cnart on the foil
package.

ASsay TIAKAN
The One Siep Saliva Alobol Test wil
Allow the pouched strip to equilibrate to room iemperature (15-27C) prior to testing react wath methyl, ethyl and allyl alcohols
1. Abstain from piacing anything in tlic moulh lar fiteen (i5) minuics prioi lo
test. This includes no-alcoholic drnks, lobucco pioducts, cot+ce. brcath
beginhing nE
munis and Juo, NRRINGSNESING
clc. And then spit Enough siall va inio lie colleclion cup The fullouing
substanves ay mkorter w the uc Scp
sampies olher than saliva The nancd substances do Saluvaappc
9NNAly
Alaohel Tes »hcn ung
n sufhctent qeantity
2. Open the foil package anu Femove ihe lest card. Obaurve the 1eactive pads on the ccl
saliva io iniertere watb th kest
1 300003