PREPARATION AND EVALUATION OF PERIODONTAL FILMS CONTAINING

AMOXICILLIN
Rahul Chaudhary*, Gauree Kukreti
Department of Pharmaceutics, School of Pharmaceutical Sciences and Technology, Sardar Bhagwan
Singh University, Balawala, Dehradun, Uttarakhand, India. 248161
Table:1 Formulation of periodontal films Table 3: Weight variation and average surface pH
INTRODUCTION S. Ingre- F1 F2 F3 F4 F5 F6 F7 F8 F9
Periodontal disease arises due to combined state of NO dients
.
S. No. Formulations Weight Average
gingivitis and periodontitis. Gingivitis is the inflammation variation surface pH
of gingiva surrounding the tooth and periodontitis is 1 Chitosan 100 100 100 200 200 200 300 300 300
mg mg mg mg mg mg mg mg mg (n=3) (mg) (n=3)
recognized by the space or pocket formation deeply inside 1 F1 11.46±0.4 6.6
tissue. The environment in the periodontal pocket is 2 Alginate 100 200 300 100 200 300 100 200 300
preferred as effective for bacterial activity as well as mg mg mg mg mg mg mg mg mg 2 F2 10.96±0.4 6.7
growth. Periodontitis is a long term occurring disease and
3 Propyl- 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml
the long term action of these bacteria slowly leads to the 3 F3 13.6±0.7 6.6
ene
demolition of periodontal tissue and results in the gradual glycol
attachment loss along with the desolation of alveolar bone. 4 F4 13.23±0.4 6.6
4 Acetic 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3
The actual purpose of this project is to prepare and acid ml ml ml ml ml ml ml ml ml 5 F5 12.63±0.8 6.7
evaluate periodontal films containing amoxicillin that must
show dual release of drug, immediate followed by sustain 5 Amoxicil 245 245 245 245 245 245 245 245 245 6 F6 13±0.6 6.7
release for the treatment of periodontitis. Amoxicillin lin mg mg mg mg mg mg mg mg mg
belongs to BCS class I drug and has biological half life of 7 F7 14.21±0.7 6.5
about 1h that means frequent dosing is necessary. In this 6 Distilled 20 ml 20 ml 20 ml 20 ml 20 ml 20 ml 20 ml 20 ml 20 ml
approach amoxicillin will be incorporated into water q.s q.s q.s q.s q.s q.s q.s q.s q.s 8 F8 12.93±0.5 6.8
polyelectrolyte complex films composed of:
9 F9 13.21±0.8 6.7
Chitosan-alginate polyelectrolyte complex (PEC) layer.
The film is meant to be delivered locally directly into the Result and discussion
Table 4: Thickness and folding endurance
periodontal pocket by which high concentration of drug
will be achieved with minimum adverse effect and due to 120

prolonged release of drug, frequency of dosing can be S. NO. Formulations Thickness Folding
minimized. Systemic treatment has many side effects, such 100 f1 f2 endurance
as hypersensitivity, even systemic uptake may develop (mm)
f3 f4

resistance to bacteria. Hence, incorporation of amoxicillin 80

1 F1 275 0.1
into different biodegradable cationic and anionic polymers f5 f6

2 F2 269 0.1
% CDR

60

will result into a PEC film that can control the drug release f7 f8
3 F3 280 0.1
maintaining its therapeutic action for prolonged period. 40

The film can be inserted into the periodontal pocket and f9 4 F4 289 0.1
show its action at the infected site which can be a better 20
5 F5 257 0.1
approach than the systemic treatment for periodontitis. 0
6 F6 272 0.1
0 10 20 30 40 50 60 70 80
Time (hr)
7 F7 263 0.1
8 F8 254 0.1
Fig. 2: Graph showing drug release of
MATERIAL AND METHODS formulations(F1-F9) 9 F9 271 0.1

Preparation of periodontal films: Table 2: Table showing % CDR

Solvent casting technique has been used to prepare the
CONCLUSION
film. Film forming polymer, plasticizer, other excipients The obtained result show that the prepared intra pocket
FORMULATIONS % CDR
will be mixed in a suitable solvent and casted into a glass periodontal films of Amoxicillin 2.5mg has immediate release
moulds or petri plates and left for drying in undisturbed followed by prolonged release rate. Among nine formulation of
manner to form a film. F1 89.32 films , F3 and F5 films can be further optimized and can be
Chitosan solution was prepared by moistening the F2 87.30 used for periodontal therapy with reduced dose and high
estimated amount of polymer in very less amount of acetic potency than systemic medication.
F3 94.43
acid overnight, for complete hydration and swelling of
polymer. Sodium alginate was also soaked overnight in F4 80.61
distilled water. In order to prepare polyelectrolyte complex
REFERENCE
F5 96.31
Kilicarslan M., et al., European Journal of
PEC film, sodium alginate solution including drug was
F6 81.13 Pharmaceutical Sciences, 123, pp. 441-451
gradually added to the chitosan solution with continuous
F7 73.14 , 2018
stirring using glass rod. The mixture was left to stand until
Kassem A. A., et al., Pharmaceutical Development
all air bubble disappeared and casted horizontally in a petri F8 76.56
and Technology, pp. 1-9, 2014
plate. The system was dried and obtained films were cut of F9 79.11
its appropriate size i.e of 7mm.