Professional Documents
Culture Documents
Service Manual
NOTES
Revision Status
NA NA
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Qualifications
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Copyright 2005–2007 by Abbott Laboratories.
All rights reserved.
Introduction
The m2000sp is an automated system designed to perform
nucleic acid sample preparation through the utilization of
general purpose reagents and an automated liquid handling
platform.
Service Manual
The information was developed to be used by Abbott
Molecular-trained personnel, by other persons knowledgeable
or experienced with the operation and service of the product
identified, or under the direct supervision and with
cooperation from Abbott Laboratories technical sales or service
representatives.
Biosafety Procedures
Use appropriate biosafety practices when handling specimens,
reagents, and contaminated instruments. Refer to Section 2,
Safety for more information.
• Consider all clinical specimens, reagents, controls, and
calibrators that contain human-sourced material and
instrument surfaces or components that have come in
contact with human-sourced material as potentially
infectious. Wear gloves, lab coats, and safety glasses, and
follow other biosafety practices as specified in the OSHA
Bloodborne Pathogens Standard 29 CFR 1910.1030, or
other equivalent biosafety procedures.
• The probes are sharp and potentially contaminated with
infectious materials. Avoid contact with tips (DiTis) of the
probes.
• Dispose of all clinical specimens, reagents, controls,
calibrators, standards (as appropriate), cuvettes, (DiTis)
and other disposables that may be contaminated, in
accordance with local, state, and federal regulations
governing the treatment of regulated medical waste.
Sharps
Sharps, such as contaminated probes, must be placed in an
appropriately marked, puncture-resistant container prior to
treatment and disposal.
Spills
Clean spills of potentially infectious materials and
contaminated instrument components with an appropriate
disinfectant, such as 0.5% sodium hypochlorite, or other
suitable disinfectant.
WARNING: Never use straight household bleach when
cleaning or maintaining the m2000sp. Do not mix any
oxidizing agents, such as sodium hypochlorite in
quantities higher than 0.5%, with the liquid waste of
the m2000sp. Toxic gases could be generated from these
mixtures. Pressure can build up in the closed container
of the mixtures.
Chemical Hazards
Operators may be exposed to hazardous chemicals when
handling reagents, calibrators, controls, or bulk solutions.
Exposure to hazardous chemicals is minimized by following
the instructions provided in the assay-specific package inserts
and product-specific Material Safety Data Sheets (MSDS).
Exposure levels are further reduced by the “hands-off” design
features of the instrument when it is used properly.
In general, observe the following precautions when handling
chemicals:
• Consult Material Safety Data Sheets for safe use
instructions and precautions.
• Avoid contact with skin and eyes. If contact with material
is anticipated, wear impervious gloves and protective
eyewear and clothing.
• Always maintain good housekeeping. Do not eat, drink, or
store food and beverages in areas where chemicals are
used.
• If irritation or signs of toxicity occur after exposure, seek
medical attention.
Table of Contents
NOTES
NOTES
Table of Contents
TOC
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Purpose of This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Target Groups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Field Service Engineers . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Qualification, Training . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Conventions Used in This Manual . . . . . . . . . . . . . . . . . . . . 1-7
Abbreviations Used in This Manual . . . . . . . . . . . . . . . . . . . 1-8
Reference Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10
Introduction
Target Groups
This manual addresses one target group: FSEs who have access
to most implemented functions.
Some functions are only accessible to Abbott production and
developers.
Functions intended only for FSEs are protected by password.
Qualification, Training
An FSE must have attended an m2000sp Service Training
course. Installation, servicing, and repair of an instrument
should only be performed by Abbott-authorized service
personnel.
The FSE must be qualified to operate a PC and be familiar with
basic operation of the Windows operating systems.
Icons
The following informational symbols are used in this manual:
NOTE: This icon indicates important information or an
exception to conventional methods.
Password Protection: This icon indicates a procedure
that is exclusively for FSEs and requires the use of a
password.
IMPORTANT: This icon is used to indicate information
! that is necessary to the proper functioning of the
m2000sp.
Refer to Notices and Symbols on page 2–5 of Section 2 for a
complete listing and description of safety symbols used in this
manual.
Abbreviation Description
APC Active Positioning Carrier
CAN Controller Area Network
CAN-BUS Controller Area Network (pathway) over
which data travels
CCW Counterclockwise
CD Compact Disk
CRC Carrier, Rack, and Container, e.g CRC-Editor
CU Control Unit, e.g., Genesis CU, Device CU
CW Clockwise
DiTi Disposable Tip
DLL Dynamic Link Library
DMSO Dimethyl Sulfur Oxide
EEPROM Electrically Erasable Read-only Memory
FaWa Fast Wash Pump
FSE Field Service Engineer
GSS Abbott Molecular Global Service and Support
ILID Integrated Liquid Detection
Licos Liquid Container Supervisor
LiHa Liquid Handling Arm
LLD Liquid Level Detection
M&D Maintenance and Diagnostics
MIO Monitored Incubator Option
MPO Monitored Pump Option
MSDS Material Safety Data Sheet
NAT Nucleic Acid Testing
NATMBS Nucleic Acid Testing Magnetic Bead
Separation
NPS Nano Pipetting System
ODBC Open Database Connectivity
OPTIBO Option Board
PC Personal Computer (also called “SCC”)
PCB Printed Circuit Board
Abbreviation Description
PosID Positive Identification
PWR Power
RAM Random Access Memory
RMP Robotic Microplate Processor
ROM Read-only Memory
RoMa Robotic Manipulator Arm
RSP Robotic Sample Processor
RWS Robotic Workstation
S&S Setup and Service
SMIO Smart Monitored Input/Output
SMIO-SAFY Smart Monitored Input/Output Safety
SN Serial Number
SVGA Super Video Graphics Adapter
Te-MagS (Tecan) Magnetic Separation Module
Te-MO (Tecan) Multipipetting Option
TEMPO Temperature Measuring Option
Te-VacS (Tecan) Vacuum Separator Module
TOC Table of Contents Link
UPS Uninterruptible Power Source
UST Ultrasonic Transducer
VCC Volume Control Center
WRC Wash Refill Center
Reference Documents
SECTION 2:
SAFETY
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Significance of These Safety Instructions . . . . . . . . . . . . . . . 2-4
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Notices and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
WARNINGS Used in This Manual . . . . . . . . . . . . . . . . . . 2-5
CAUTIONS Used in This Manual . . . . . . . . . . . . . . . . . . 2-5
Warning Notices Attached to the Product or Its
Surroundings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Product Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Use of the Product . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Safety of the Overall System . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
Instrument-related Hazards and Safety Measures . . . . . 2-8
Other Hazards and Safety Measures . . . . . . . . . . . . . . . 2-10
Removal of Safety Elements . . . . . . . . . . . . . . . . . . . . . 2-12
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
When to Decontaminate . . . . . . . . . . . . . . . . . . . . . . . 2-13
Decontamination Method . . . . . . . . . . . . . . . . . . . . . . 2-13
Decontamination Form . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Field Decontamination Checklist . . . . . . . . . . . . . . . . . 2-15
General Safety Rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Legal Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Duty of Maintenance and Care . . . . . . . . . . . . . . . . . . 2-16
Spare Parts to be Used . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Introduction
This section summarizes the safety instructions applicable to
the m2000sp, and those to be observed when installing,
servicing, or upgrading an instrument. Operation,
maintenance, and servicing of the m2000sp could expose
individuals to potential safety and health hazards. All service
must be performed in accordance with procedures described
herein. This section describes the types and locations of
potential hazards that could cause physical harm to an
operator or service personnel. Warnings and cautions are
provided to alert operators and service personnel of potential
hazards. Standard warning conventions, including hazard
signal words or symbols are described in subsequent sections.
Symbol Significance
Laser Hazard
Identifies a Class 2 laser product that contains an
embedded low power visible Class 2 laser diode bar
code scanner. Warns against direct viewing into the
bar code laser beam or reflections of the beam.
Potentially Infectious
Indicates an area where the FSE or operator could
be exposed to potentially infectious human-sourced
material. Contamination hazards exist if parts of
the instrument are not completely decontaminated.
Wear appropriate personal protective equipment
such as gloves, lab coat, and protective eyewear.
Hot Surface
Indicates an area where the equipment reaches very
high temperatures and caution must be used to
prevent injury.
Pinch Point
Indicates an area where moving parts have the
potential to cause injury if caution is not taken.
Product Safety
Use of the Product
The m2000sp instrument is a fully automated system that
performs nucleic acid sample preparation through the use of
general purpose reagents and an automated liquid handling
platform.
Principle
The m2000sp is designed and built in accordance with current
state-of-the-art technology and recognized technical safety
regulations. However, there are risks to users, property, and the
environment if the instrument is used carelessly or improperly.
Warnings in the m2000sp Operations Manual and m2000sp
Service Manual alert the user to these potential dangers.
Decontamination
When to Decontaminate
In addition to regular decontamination, the user must
thoroughly decontaminate the instrument according to
standard laboratory regulations in the following cases:
• Before any maintenance or service is performed on the
instrument
• Before an Abbott FSE performs on-site service on the
instrument
• Before the instrument is returned to Abbott (e.g., for
repair)
• Prior to storage of the instrument
• Prior to disposal of the instrument or parts of it
• In most cases, before the instrument or individual parts
leave the user’s site
Decontamination Method
The decontamination method must be adapted to the
respective application and associated substances used by the
operator. The owner assumes full responsibility for appropriate
decontamination of the entire instrument.
Refer to the m2000sp Operations Manual for specific
decontamination instructions.
WARNING: Potential Biohazard. Contamination
hazards exist if parts of the instrument are not
completely decontaminated. Components with direct
contact (or the potential for direct or accidental
contact) with chemical or biological material must be
treated, including the tubing system and all upstream
equipment.
Decontamination Form
Before an Abbott FSE performs any service on the instrument
or before the instrument is returned to Abbott Molecular, the
instrument owner must confirm in writing that
decontamination was performed properly and in accordance
with good laboratory practice guidelines. For this, the owner
must enclose the declaration (Field Decontamination
Checklist), provided below.
Abbott Molecular will refuse any instrument, or part of it, if
the decontamination form is not completed and duly signed.
Refer to the m2000sp Operations Manual for specific
decontamination instructions.
Please mark:
I. X for completed
ITEM DESCRIPTION II. N/A if not required by
operations manual
1 Remove all specimens.
2 Remove all reagents.
3 Decontaminate/flush fluid pathways with buffer or other fluid if indicated.
4 Empty and rinse out waste container(s).
5 Remove and drain hoses. Plug drain holes as necessary.
6 Wipe surface of instrument with a detergent solution.
7 Wipe surface of the instrument with an appropriate disinfectant per the
operators manual
8 Affix this Field Decontamination Checklist to the instrument
Completed by:
___________________________ ___________________________ _____________________ ________
Signature Printed Name Title Date
This information is confidential to Abbott. The user is responsible for confirming use of the current Issued Version prior to use.
Modifications
Modifications to the m2000sp are permitted only after prior
consultation with and written approval from the
manufacturer. Modifications and upgrades shall be carried out
by an authorized field service engineer only. The manufacturer
declines any claims resulting from unauthorized
modifications.
SECTION 3:
TECHNICAL DATA
NOTES
Table of Contents
System
The m2000sp system is a fully automated system for sample
preparation. The system automates nucleic acid sample
preparation through utilization of general purpose reagents
and an automated liquid handling platform. The operator
controls the system with a personal computer (PC) equipped
with service and operating software. There are three distinct
processes involved in amplified nucleic probe technology:
• specimen preparation
• nucleic acid amplification
• automated amplified nucleic acid detection
The m2000sp system performs the first process, specimen
preparation.
The Liquid Handling Arm (LiHa) has eight sampling tips
arranged on one arm. The LiHa moves left and right (X-range),
and is driven by a Servo motor. Each sampling tip is raised or
lowered by its own Servo motor within the arm. Two
additional Servo motors inside the LiHa arm drive the tips
forward and backward and control the Y-spacing of tips. The
LiHa has independent movement in the Z-direction, and also
has equidistant movement in the Y-direction, from 9 to 38
mm maximum (0.31 to 1.5 in) between tips. The resolution is
0.1 mm in all three axes (X, Y, Z).
Disposable filtered 200 µL and 1000 µl pipette tips are used.
Integral detectors (on the LiHa arm) ensure tip attachment.
While using disposable tips, electronics for tip presence check
can control each Z-axis individually, independent of the
presence or absence of a disposable tip.
The liquid system is a central component of the instrument.
Flexible tubing connects the containers, pumps, valves, and
tips. Precision diluters (syringes) ensure accurate aspiration
and dispensing of liquids and air gaps, the latter to separate
various liquids. System liquid is aspirated from the system
liquid container through aspirating tubing, via liquid
distributors to the diluters.
The PosID module reads both horizontal and vertical bar codes
for positive identification and positioning of carriers, racks,
and containers. With its gripper, it pulls carriers toward the
rear of the instrument for bar code identification on tubes
then pushes the carrier back into position. Commonly
available bar codes can be read. The presence of a check digit
may or may not be required. For tube racks, a tube sensor
detects the presence of a tube.
Table 3.1: Bar Code Specifications
Description Specification
Readable Sample Bar Code 39 (length flexible)
Codes Codabar (length flexible)
Code 128 (length flexible)
2 of 5 Interleaved (length 2-20 even)
Code 93 (length flexible)
UPCA (length flexible)
Bar Code Length Up to 20 characters, depending on
symbology
Bar Code Density (0.127 to 0.381 mm) (5 to 15 mils)
Print Contrast Ratio 70%
PCS
Bar Code Height 욷8 mm
Bar Code Length Max of 64 mm (without quiet zone)
Speed Pos ID version 2 moves in
X-direction along the worktable
with a speed of 40 cm/sec
Wavelength 670 nm
Laser Class Class 2
Quiet Zone 5 mm on either side of bars
Description Specification
Rotation 270
Angle
Gripper 58 to 140 mm
Range
Precision Below ± 0.4 mm in all directions
Force Maximum force of the RoMa Arm in the
Z-direction is approximately 35 N, gripper
force is adjustable to 4 N maximum. Default
gripper force is 1.4 N.
The Output Deck is the site of the Peltier chiller, assay reagent
packs, master mix tubes, PCR plates and deep well plates used
during instrument operation. The Output Deck is mounted on
the right side of the instrument. The chiller is maintained at a
temperature range between 5°C and 30°C, ± 3°C, when
required by the Application Specification.
In addition, some instruments include hoods that cover the
worktable. However, hoods are not standard equipment on all
G-series instruments.
Instrument
Table 3.3: Instrument Dimensions
Description Specification
Length 142.9 cm/56.3 in
Length (side service door 176.5 cm/69.5 in
open)
Depth 76.8 cm/30.3 in
Depth (front lid open) 124.5 cm/49.0 in
Height (RoMa extended) 92.0 cm/36.2 in
Height (with hood) 168.4 cm/66.3 in
Weight* 189.3 kg/417.3 lb
Weight (with hood)* 206 kg/454 lb
Description Specification
Clearance 1 m (39 in) around the
instrument
10.2 cm (4 in) minimum
for rear clearance
Cabinet
Table 3.5: Cabinet Specifications
Description Specification
Length 142.9 cm/56.3 in
Depth 79.4 cm/31.3 in
Height 79.5 cm/31.3 in
Weight 103.4 kg/228.0 lb
Worktable Dimensions
Table 3.6: Worktable Dimensions
Description Specification
Accessible X-range (X-travel) 1120 mm/44.1 in
Accessible Y-range (Y-travel) 418 mm/16.5 in
Grid Positions on Worktable 45
Cutout at Grid 18 for Wash/waste Station
Z-Ranges
Table 3.7: Z-Ranges
Description Specification
RoMa Standard 259 mm/10.2 in
LiHa 165 mm/6.5 in (with
standard tips)
1 2 Screw Position 3 4
Weights
Table 3.8: Weights
Description Specification
LiHa 8 kg/18 lb
RoMa Standard and Gripper 6 kg/13 lb
PosID 11 kg/24 lb
FaWa 2 kg/4.4 lb
Electrical Supply
Table 3.9: Electrical Supply
Description Specification
Line Voltage 100-120 VAC/ 220-240
VAC ± 10%
Frequency 50-60 Hz
Power 1200 VA
Equipment and Safety Class I
Circuit Breaker 15 A
Electrical Outlets Required Minimum of 4:
• instrument
• SCC
• monitor
• printer
System Liquid
System liquid is supplied directly from the system liquid
bottle. Use distilled or deionized water.
Environmental Conditions
The m2000sp instrument is intended only for indoor operation
and storage.
Table 3.10: Environmental Conditions
Description Specification
Operation 15 to 30°C/59 to 86°F
Temperature
Operation Humidity 30% to 80% relative (non-
condensing) at 30°C/86°F or below
Pollution Degree 2 (Non-specific environment
described as a typical laboratory)
Storage Conditions
Protect the instrument from dust and debris with a cover.
Recommendation: store the m2000sp in its original packaging.
Computer Requirements
The computer provided by Abbott Molecular meets or exceeds
minimum requirements to operate the instrument.
Site Requirements
Table 3.11: Site Requirements
Description Specification
Loading Dock Present
Load Capacity of At least 411 kg/907 lbs
Elevator
Minimum Size of Refer to Table 3.4: Instrument
Elevator Clearance on page 3–10 and
Table 3.5: Cabinet Specifications
on page 3–11
Door and Entryway Greater than 815 mm/32 in
Openings
Passageway Corners Combined total width of both
passageways around corners must
be greater than 2,794 mm/110 in
Settings
Address Settings Overview
Table 3.12: Address Settings
Address
Module Board
Setting
Access SMIO 2
Option SMIO/SAFY 1
LiHa DC Servo 1 (position 1) * 4
DC Servo 2 (position 2) 3
DC Servo 3 (position 3) 2
DC Servo 4 (position 4) 1
DC Servo 5 (position 5) 0
DC Servo power (position 6) 5
RoMa RoMa Freedom Backplane CU 4
Standard RoMa Freedom Backplane PWR 0
RoMa 2 Servo Y/Z 6
RoMa 2 Servo R/G 7
Diluters Diluter 1 0
Diluter 2 1
Diluter 3 2
Diluter 4 3
Diluter 5 4
Diluter 6 5
Diluter 7 6
Diluter 8 7
FaWa MPO 0
PosID DC Servo (CU PosID) 0
Y/B 2
1 mL CC Mags #3 3
Subsystem CC Mags #4 4
Jumper Settings
(m2000sp terminated)
Y2 Y1
Z6 (2) Z3 (3)
Z2 (-) Z7 (-)
Z8 (-) Z1 (-)
Z4 (4) Z5 (1)
X1 ---
-----
(m2000sp terminated)
-----
SW3
Device CU Adress Switch
#4 = C1
#5 = C3
(m2000sp terminated)
SMIO
J2 CAN-BUS
J9 Jumper (outer pins jumpered)
J18 System liquid sensor
J19 Liquid waste sensor
J21 Solid waste sensor
SW1 Address switch
(m2000sp terminated)
SMIO-SAFY
J2 CAN-BUS to OPTIBO Bd
J3 CAN-BUS to SMIO Bd
J24/J5LLeft door lock
J23/J6RRight door lock
J9 Jumper (inner pins jumpered)
SW1 Address switch
(m2000sp not terminated)
(m2000sp not
terminated)
SW1 and SW2 OFF
Error States CU
Table 3.13: Error States
Green Red Error
Stays on/off Stays on/off Fatal hardware error
Blinking Off Normal operation
Blinking On Flash not accessible
Blinking Blinking Power fail circuit failure
Blinking fast Blinking fast Firmware download
required
Cable Connections
Table 3.14: Cable Connections: From/To
From To
Connection Cable Connection Board
Power Module (0760510)
24 V 2008664 J2 Power In 1 Optibo
Freedom
24 V 2008664 J14 Power In Optibo
2 Freedom
J1 Line In 100 to 2240
L/N/PE VAC
50/60 Hz
From To
Connection Cable Connection Board
OPTIBO Freedom (2010113)
J4 2008582 J1 S-CAN IN VCC-DILBACK
Diluter/S-CAN 8 (2008555)
J2 Power In 1 2008664 24V Power Module
(0760510)
J14 Power In 2 2008664 24V Power Module
(0760510)
J1 RS232 2008588 RS232 Multiport
J7 CAN 2008583 J7 O-CAN 1 MPO (FaWa)
Option 1 (2009001)
J8 CAN 2010004 J10 O-CAN 1 CU-PosID
Option 2 (2009457)
J9 CAN 2010406 J1 O-CAN 1 C.C. MagS
Option 3 (2009606)
J10 CAN 2010406 J1 O-CAN 1 C.C. MagS
Option 4 (2009606)
J5 Flex 13 2010278 J1 S-CAN LiHa Freedom
S-CAN Backplane
LiHa only (LiHa)
(2010104)
J15 Flex 13 2010156 J1 Option RoMa
O-CAN (Arm CAN Backplane
R1) (2010111)
J18 O-CAN 2010209 J2 O-CAN SMIO-SAFY
RJ45 (2010225)
VCC-DILBACK 8 (2008555)
J1 S-CAN IN 2008582 J4 Optibo
Diluter/S-CA Freedom
N (2010113)
J38 VCC-1401 2010211 J12 ILID Freedom
(DIL 1-4) (NPS/VCC) (2010158)
(Tip 1-4)
J39 2010211 J13 ILID Freedom
VCC-ILID2 (NPS/VCC) (2010158)
(DIL 5-8) (Tip 5-8)
From To
Connection Cable Connection Board
LiHa Freedom Backplane (2010104)
J4 Y1 Motor 2008617
J5 Y2 Motor 2008617
J6 Z3 Motor 2008615
J7 Z6 Motor 2008615
J8 Z7 Motor 2008615
J9 Z2 Motor 2008615
J10 Z1 Motor 2008615
J11 Z8 Motor 2008615
J12 Z5 Motor 2008615
J13 Z4 Motor 2008615
J3 Lower Diti 2009603
Eject
J16 X Motor 2010184
J1 SYS-CAN 2010278 J5 (S-CAN) Optibo
Freedom
(2010113)
J2 2010186 J9 ILID Freedom
(2010158)
ILID Freedom (2010158)
J9 2010186 J2 LiHa Freedom
Backplane
(2010104)
J22 NPS/J23 2010183 J1 Tip Adapter
Tip Z1 Board
(2010149)
From To
Connection Cable Connection Board
J24 NPS/J25 2010183 J1 Tip Adapter
Tip Z2 Board
(2010149)
J26 NPS/J27 2010183 J1 Tip Adapter
Tip Z3 Board
(2010149)
J28 NPS/J29 2010183 J1 Tip Adapter
Tip Z4 Board
(2010149)
J30 NPS/J31 2010183 J1 Tip Adapter
Tip Z5 Board
(2010149)
J32 NPS/J33 2010183 J1 Tip Adapter
Tip Z6 Board
(2010149)
J34 NPS/J35 2010183 J1 Tip Adapter
Tip Board
Z7 (2010149)
J36 NPS/J37 2010183 J1 Tip Adapter
Tip Board
Z8 (2010149)
J38 GND
J39 GND
SW1/SW2 set to pos “8”
RoMa Freedom Backplane (2010111)
J10 X-motor X-motor
(2010184)
J1 Option 2010156 J15 O-CAN Optibo
CAN Flex 13 Freedom
(2010113)
From To
Connection Cable Connection Board
RoMa 2 Backplane (2008783)
ST1 / Local 2010144 J2 Local CAN RoMa Freedom
CAN Backplane
(2010111)
ST9 / Z-Brake Z Brake
(2008782)
ST4 / Z-Motor Z Motor
(2008808)
ST3 / Y-Motor Y Motor
(2008615)
ST6 / Signal 2008815 J4/Signal Gripper
(RoMa) (2008794)
2010143
(GRoMa)
ST5 / Power 2008815 J3/Power Gripper
(RoMa) (2008794)
2010143
(GRoMa)
Gripper (2008794)
J1 / G-Motor 2009372 G Motor
(2009371)
J2 / R-Motor R Motor
(2008809)
J3 / Power 2008815 ST5/Power RoMa 2
(RoMa) Backplane
2010143 (2008783)
(GRoMa)
J4 / Signal 2008815 ST6/Signal RoMa 2
(RoMa) Backplane
2010143 (2008783)
(GRoMa)
From To
Connection Cable Connection Board
1 mL Subsystem
J1 O-CAN 1 2010406 J9 O-CAN 3 Optibo
Freedom
(2010113)
J1 O-CAN 1 2010406 J10 O-CAN 4 Optibo
Freedom
(2010113)
J11 Out 2009610 Heating Plate
(2009625)
J11 Out 2009610 Heating Plate
(2009625)
SMIO-SAFY (2010225)
J2 O-CAN 2010209 J18 O-CAN Optibo
RJ45 Freedom
(2010113)
J3 O-CAN 2010407 J2 O-CAN SMIO
(2009706)
J5/J24 Door 2010178 Door lock 1
(left) (0750270)
J6/J23 Door 2010177 Door lock 2
(right)
(0750270)
SMIO (2009706)
J2 O-CAN 2010407 J3 O-CAN SMIO-SAFY
(2010225)
J18 IN 1 level sensor
system liquid
(2010411)
J19 IN 2 level sensor
system waste
(2010411)
J21 IN 4 level sensor
solid waste
(2010410)
Fuse Location
Sub-assembly
OPTIBO Board Fuse Size
Protected
(Top to Bottom)
FU1 T 6.3A CU Board
FU6 T 8A Option 1 FaWa
FU7 T 8A Option 2 PosID
FU8 T 8A 1 mL Subsystem
FU9 T 8A 1 mL Subsystem
FU4 T 8A Diluter
FU3 T 8A RoMa/R1 R2
FU2 T 8A LiHa
FU5 T 8A Option 5
FU10 T 8A Arm L1 L2
Motor
619854 1 1
X-Drive
Assy
200942-103—September 2007
m2000sp G-series Service Manual
Section 3
Section 3 Diagrams
Diagrams
This section includes all relevant diagrams, such as block
diagrams, wiring diagrams, etc., that are useful for
maintenance or repair.
Wiring Diagrams
3-35
Press 1-1
PIEZO-Module Diluter 1
S-CAN
DIL1
J28
J20
J2
2008556
S-CAN IN
2008582 (150)
Optibo freedom
J12
I/O
2008901 (200)
D1 / #0
Press 1-2
PIEZO-Module Diluter 2
S-CAN
DIL2
J21
J29
J3
2008556 XP 3000+
S-CAN OUT
729556
J13
I/O
D2 / #1
J11
Press 1-3
PIEZO-Module Diluter 3
S-CAN
DIL3
J22
J30
J4
2008556 XP 3000+
729556
J14
I/O
D3 / #2
Press 1-4
PIEZO-Module
VCC-ILID2 (DIL5-8)
Diluter 4
S-CAN
DIL4
J23
J31
J5
2008556 XP 3000+
J39
729556 2010211
ILID freedom
J15
I/O
D4 / #3 J13
Press 1-5
PIEZO-Module Diluter 5
J32
J6
VCC-ILID1 (DIL1-4)
2008556 XP 3000+
729556
J16
I/O
ILID freedom
J38
D5 / #4 2010211
J12
Press 1-6
PIEZO-Module Diluter 6
S-CAN
DIL6
J25
J33
J7
2008556 XP 3000+
729556
J17
I/O
D6 / #5
Pressure2
J37
Press 1-7
PIEZO-Module Diluter 7
S-CAN
DIL7
J26
J34
J8
2008556 XP 3000+
729556
J18
I/O
D7 / #6
LVD-Print
2010212
Press 1-8
PIEZO-Module Diluter 8 J1
S-CAN
LoVol
DIL8
J27
J35
J9
J36
2008556 XP 3000+
200942-103—September 2007
729556
Misc Definition Signals Grounding
J19
I/O
D8 / #7
24V => Power 24V
Signal Tower
SECTION 4:
INSTALLATION
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
G-series Installation Checklist. . . . . . . . . . . . . . . . . . . . . . . . 4-6
Site Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-13
Pre-Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Cabinet Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-17
Unpacking and Positioning . . . . . . . . . . . . . . . . . . . . . 4-17
Cabinet Leveling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-19
Instrument Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-21
Unpacking the Instrument . . . . . . . . . . . . . . . . . . . . . . 4-22
Lifting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . 4-22
Instrument Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-27
CAN-BUS Resistance Test . . . . . . . . . . . . . . . . . . . . . . . 4-33
Instrument Setup Completion . . . . . . . . . . . . . . . . . . . 4-34
Installation of 1 mL Subsystem . . . . . . . . . . . . . . . . . . 4-39
Hood Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-40
SCC Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-41
Printer Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-42
Installation of Instrument Software . . . . . . . . . . . . . . . . . . 4-43
Hardware Configuration . . . . . . . . . . . . . . . . . . . . . . . . 4-44
Instrument Status Screen . . . . . . . . . . . . . . . . . . . . . . . 4-45
Genesis Setup and Service Software . . . . . . . . . . . . . . . 4-46
Configure the System . . . . . . . . . . . . . . . . . . . . . . . . . . 4-47
Configure the LiHa . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-53
Configure the RoMa . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-74
Configuring Sample Bar Code Labels . . . . . . . . . . . . . . 4-86
Configure the PosID 2 . . . . . . . . . . . . . . . . . . . . . . . . . 4-87
Installation of Wash/Waste Station . . . . . . . . . . . . . . 4-104
Calibrate and Verify System Liquid, Liquid Waste, and
Solid Waste Sensors . . . . . . . . . . . . . . . . . . . . . . . . . 4-105
Reinstallation of the Output Deck . . . . . . . . . . . . . . . 4-115
Perform Peltier Chiller Temperature Check . . . . . . . . 4-115
Check Door Locks . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-115
Verify Temperature Performance of 1 mL Subsystem Heater
Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-119
Set Up Liquid System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-121
FaWa Pump Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-121
FaWa Fill/Flush Protocol . . . . . . . . . . . . . . . . . . . . . . . 4-122
Elimination of Bubbles . . . . . . . . . . . . . . . . . . . . . . . . 4-123
Perform FaWa Pump Test . . . . . . . . . . . . . . . . . . . . . . 4-123
Perform Liquid Detection . . . . . . . . . . . . . . . . . . . . . . 4-127
Perform Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-131
Exiting Setup and Service . . . . . . . . . . . . . . . . . . . . . . 4-133
Customer:
State: Zip:
Customer (print)
Site Inspection
Task Reference Complete
Pre-installation inspection checklist
Pre-Installation Checklist on page 4–14
obtained and reviewed
Tip adapter test performed Check Each of the Tip Adapters on page 4–58
LiHa reference positions determined Determine LiHa Reference Position on page 4–60
Lower DiTi eject calibrated Calibrate the Lower DiTi Eject on page 4–63
Lower DiTi Eject Test performed Perform the Lower DiTi Eject Test on page 4–64
Basic mechanical accuracy of the PosID Verify Basic Mechanical Accuracy on page 4–91
confirmed
PosID 2 setup performed Set Up PosID 2 on page 4–92
FaWa pump test performed Perform FaWa Pump Test on page 4–123
Calibrations – LiHa
Task Reference Complete
Tip Adapter calibrations performed Calibrate Tip Adapter Locations on page 4–136
Reference Tip calibrations performed Calibrate Reference Tip Locations on page 4–139
Calibrations – RoMa
Task Reference Complete
Calibration of the 1 mL Subsystem 1 mL Subsystem Calibration on page 4–158
performed
Calibration of the DiTi carrier racks Disposable Tip Rack Calibration on page 4–161
performed
Calibration of the DiTi shelves Shelf Calibration on page 4–165
performed
DiTi Rack Tests – Deck and Shelf RoMa DiTi Rack Test on page 5–27 and
performed RoMa DiTi Shelf Test on page 5–29 of
Section 5, Maintenance and Diagnostic
Procedures
RoMa 1 mL Carrier Test performed RoMa 1 mL Carrier Test on page 5–30 of
Section 5, Maintenance and Diagnostic
Procedures
Sample Extraction Test Sample Extraction Test on page 5–37 of
Section 5, Maintenance and Diagnostic
Procedures
Z-Calibration Check (Sample Z-Calibration Check – Sample Extraction on
Extraction and Master Mix Addition) page 5–40 and Z-Calibration Check – Master
performed Mix on page 5–45 of Section 5,
Maintenance and Diagnostic Procedures
On-Line Documentation
Task Reference Complete
On-line documentation loaded Refer to the Software Updates page on the
GSS website
Site Inspection
Pre-Installation Checklist
Account Information
Account Name
Delivery Address Bldg. & Floor
City State/Country Zip Code
Primary Contact Phone
Secondary Contact Phone
Customer Number Customer E-mail
Site Specifications
Instrument & Cabinet space meets specification criteria
Record space
measurements _____________ Length
_____________ Height
_____________ Depth
Weight – 293 kg (645 lb) instrument, cabinet, and accessories (310 kg/682 lb w/hood)
Notify customer the floor load capacity must be verified to see if it is adequate
If floor capacity is inadequate, resolution plan:
Page 1 of 3
Delivery Route
Measure door width openings from entryway to lab and record here: _________________________________
Check one: _____ Door and entryway openings are 102.9 cm (40.5 in) or wider
_____ Door and entryway openings are less than 102.9 cm (40.5 in) but at least 81.5 cm (32 in). In this case,
REMOVAL OF INSTRUMENT FROM PALLET MAY BE REQUIRED. Contact area customer service.
_____ Door and entryway openings are less than 81.5 cm (32 in). Contact area customer service.
Measure turning radius in passageways. The m2000sp requires a combined 279.4 cm (110 in) of width of both passageways
around corners.
Is there a loading dock? Yes _____ No _____ If No, contact area customer service
Does the loading dock have a raised delivery platform? Yes _____ No _____ If No, contact area customer service
If any of the following are answered YES, contact area customer service
Does the instrument have to be moved up or down stairs? Yes _____ # of flights __________ No _____
Does the instrument have to be moved via freight elevator? Yes _____ Elevator door width __________ No _____
Will the instrument have to be lifted over any barriers? Yes _____ Height of barrier __________ No _____
Power Requirements
Dedicated/Isolated 15 amp power line with circuit breaker identified as Abbott m2000sp System is optimal. If not possible,
how clean is the power? What other equipment is plugged into the same power line? See Uninterruptible Power Supply below.
m2000sp power outlet is within 30.5 cm (12 in) of the instrument’s electrical input panel.
Power Measurements
System Requirements: Voltage range: 100-240 VAC + 10%
NOTE—All system components Voltage frequency: 50/60 Hz + 1%
will run at the single phase voltage
standard for the country (e.g. Power: 1200 VA
Japan 100VAC + 10%, US 110VAC Circuit breaker: 15 A
+ 10%, EU 220VAC + 10%)
Page 2 of 3
Miscellaneous
System Liquid Requirements: – Internal waste container used for liquid waste recovery
– Non-plumbed distilled or deionized water is required
Check bar code type below (check all that apply)
Codabar _____ w/o Checksum Code 39 standard _____ w/ Checksum _____ w/o Checksum UPCA _____
Interleaved 2 of 5 _____ w/ Checksum _____ w/o Checksum Code 128 subset A, B, and C _____ Code 93 Standard ____
Unknown _____ Other _____ Please list ________________________________ OBTAIN A BARCODE SAMPLE
Record the type(s) of sample tube(s) that have been chosen by the customer
Type of sample tube __________________________________ Distributor/Manufacturer _________________________________
Sample tube size _____________________________________ Catalog number __________________________________________
OBTAIN one (1) of each sample tube being used by the customer
Uninterruptible Power Supply (UPS) Requirements
It is recommended that the customer use one of the following UPS:
— For the m2000sp G-series: General Electric GT1500 (110V); for 220V, contact the EMEA Support team in Germany
— For the SCC: 07D06-11 (110V) or 07D06-22 (220V)
Additional Questions
What kind of samples will be used (blood, buffer, urine, etc.)? __________________________________________________________
Will the customer be performing DNA or RNA extractions? _____________________________________________________________
Does the customer require a certificate of installation (IQ/OQ)? _________________________________________________________
Refer to the m2000sp Service Manual for more detail on any of the above information.
Questions? Call: 1.224.361.7375 or, if in Europe, the Middle East, or Africa, contact the EMEA
Service and Support team in Germany
Page 3 of 3
Cabinet Installation
Cabinet Leveling
1. Level the cabinet by performing the following (refer to
Figure 4.5 below):
• Front to back (A-B): Place a 1.22 m/4 ft bubble level on
the end of the cabinet, parallel to A-B. Adjust the
caster on the low corner until the bubble is centered
in the sight glass.
Instrument Installation
3 4
1 2
5
Instrument Setup
1. Remove the styrofoam blocks from the top ends of the
instrument, the two cardboard worktable protection
strips, styrofoam blocks wedged between the diluters and
hotel, and the cardboard pieces protecting the X-rail and
PosID.
5. Remove the tape and packing material from the left and
right sides of the cabinet.
Arrows pointing in
the direction of
flow.
7. Secure the cables to the front frame with cable clamps, two
per cable. Use a flathead screwdriver to carefully pry the
clamps from the frame.
9. Apply a green decal to the waste tray for the system liquid
container and a red decal to the waste tray for the liquid
waste container.
12. Install the liquid waste container into the right container
compartment. For ease of identification, tie-strap the two
liquid waste tubes together. Then, connect the clear
tubing with the beige quick-connect fitting to the
container cap’s beige male connection and the clear
tubing with the white quick-connect to the white male
connection.
NOTE: Seams of the bottle should face away from
the sensor.
13. Install the solid waste container, with bag for disposable
tips, into the cabinet.
Installation of 1 mL Subsystem
1. Power off the instrument.
2. Place the 1 mL Subsystem upside down on the worktable
surface. Connect the two cables to the two heater boards,
accessed through the notched opening in the bottom
cover of the 1 mL Subsystem; the cables can be connected
to either of the two connectors. The software reads the
address setting of each heater block. Secure the cables with
a screwdriver.
Hood Installation
Hoods are not standard equipment on all G-series instruments.
When installation is required, refer to the latest revision of TSB
605-018.
SCC Installation
1. Unpack the computer stand, SCC, monitor, printer,
keyboard, and mouse. Set up the computer stand then set
the SCC, monitor, printer, keyboard, and mouse in place.
Do not connect cables at this time.
Monitor
Computer (CPU)
with CD Drive
Keyboard
Mouse
2. Plug all power cables into the rear of the SCC as shown
below. Verify the correct voltage is selected on the SCC.
Printer Installation
Refer to the latest revision of the applicable printer or software
installation TSB.
Hardware Configuration
From the <System Settings> menu, select <Hardware
Configuration> then <Configure>.
Enter Password
1. Start the function with <System>, <Password>. The
<Password> dialog displays.
Password Protection: Enter the password to
access specific service-related software menu
items.
NOTE: The <System> menu includes all
commands that apply to the whole system, and
not to a particular function.
COM Port
Purpose
This function allows the communication port (e.g., COM1,
COM2, etc.) to be selected or changed. The computer
communicates with the instrument through the
communication port.
NOTE: It should not be necessary to change the port
assignment. Installation uses COM1.
Procedure
Perform the following steps to select or change a
communication port.
1. Ensure the instrument is connected to the required COM
port connector, normally located on the rear side of the
computer. Refer to the accompanying documentation for
the computer, if necessary.
Load Worktable
1. To load a worktable, select <System>, <Load Worktable>.
Refer to Load Worktable in Section 11, Genesis Setup
and Service Software for more information.
2. If available, select the <m2000sp Worktable>. Otherwise,
select the <NAT MBS Worktable>.
3. Select <OK>.
Exit/Finish Setup
Select <System >, <Exit> to close the Setup & Service software
safely without data loss.
Reference Tips
Several LiHa configuration activities require the use of a
reference tip. There are currently two types of reference tips
available:
• Long reference tip (old style)
• Short reference tip (new style)
Channel Configuration
1. Select <System Devices><LiHa>.
Reference Tip
Reference Point
5. Select <Select Tips> and verify all eight DiTis are 1000 µL
DiTis with Filters.
6. Select <OK>.
7. Select <Run> to initiate the test. The status becomes <Test
Running>.
8. At completion of the test, the status becomes <Test
Passed>.
9. Select <Close> to finish the test.
10. If the test failed:
• Verify the LiHa reference positions.
• Check the tip adapters.
• Perform individual Z alignment.
Y-Spread Procedure
Enter
A1RPY8
here
10. Using the <Single Commands> tool again, set all tips to
tip 3’s Z-height on the Y-spread tool by entering the
following command:
A1PAAX,Y,Ys,Z1,Z2,Z3,Z4,Z5,Z6,Z7,Z8
Here X, Y, and Z1-Z8 are the tip 3 values found above, and
Ys is 90 (as set by the Y-spread script).
11. Observe the placement of the tips and adjust the values to
re-center tip 3 if necessary.
Observe the Y-spread at these locations. Ideally, the tips
will not be touching the walls of the Y-spread notches.
If the tips are out of alignment, check for bent tip
adapters and replace if necessary.
If tip 3 or tip 7 are replaced, reperform the LiHa
Determine Range test (refer to Determine LiHa Range on
page 4–60) then perform the Y-spread check again.
12. Using the <Single Commands> tool, reinitialize the axis
by entering the following command:
A1PIZ
13. Run the Y-spread batch file from ISA 600-015 and do the
following:
• Enter the grid location
• Select No when asked if you will be using reference
tips
• Select Yes when asked if you will be using the front
holes
• Select Yes when asked if you will be changing the
Y-spread to 18.8 mm
14. Align tip 3 with the associated gap on the y-spread tool.
18. Move the tips back and forth over the Y-spread tool from
the front location to the back location.
21. Move back to the 188 (front) location, then back to the 90
(back) location, then the 188 location again.
90
188
• Comment
Set Type
1. On the <Contents> page, select <Set Type> then switch to
the <Setup> page.
2. Select:
• <CW> for <Rotator Type>
• <Eccentric> for <Gripper Type>
• <Normal (standard)> for <Z Range>
Determine Range
1. On the <Contents> page, select the <Determine Range>
checkbox (聺) and switch to the <Setup> page.
4. Remove the gripper arms from the RoMa head. Use the
<RoMa Move> screen to align the front line of the gripper
mount to the back of the 1 mL Subsystem or the back of
an MTP carrier (the important thing is that it is aligned to
a straight edge).
6. Check the gap between the Service Rack and gripper pin.
If needed, adjust to approximately 0.5 mm.
Set Up PosID 2
1. Start the function with <System Devices>, <PosID>,
<Setup>.
6. Slide the Service Rack into grid 1. Do not push it to its stop
position. Verify it is approximately 1 cm in front of the
stop position.
12. Slide the Service Rack into grid 41. Do not push it to its
stop position. Verify it is approximately 1 cm in front of
the stop position.
13. Confirm with <OK>. The <Move PosID: Move Gripper to
position 41> dialog displays.
14. Carefully push the Service Rack toward the rear of the
instrument to its stop position.
15. Check the position of the gripper pin against the adjusting
slot of the Service Rack. The pin must fit exactly in the slot.
Guideline
Laser Beam
Correct Incorrect
Service Rack
Horizontal Calibration
Line
Laser Beam
Correct Incorrect
Figure 4.100:Vertical/Horizontal Scanner Head Adjustment
24. If the laser is out of line, use the rotation buttons to adjust
the scanner head accordingly.
25. Confirm with <OK>. The tests for <Adjust scan line start>
and <Adjust scan line stop> are performed automatically.
PosID 2 adjustment was completed successfully.
A warning sticker
shows when the
waste chute is
being installed
incorrectly.
A green circle
appears on the
front of the waste
chute.
Calibrate and Verify System Liquid, Liquid Waste, and Solid Waste Sensors
Adjustments and calibrations are performed through the
Genesis S&S software.
NOTE: To access S&S, log on to the SCC and select
<System>, <Setup and Service>.
9. Select OK.
NOTE: If the calibration fails, check the
connection to the SMIO board and repeat the test.
NOTE: If the calibration fails again, replace the
sensor.
10. Perform the DiTi Waste Sensor Verification on page 4–114.
Sensor Verification
10. The prompt <Door Lock 1 (left) / Please close the main
door> displays. Select <OK>.
11. The prompt <Door Lock 1 (left) / Check if the left side
is now locked> displays. Select <OK> if successful; if
unsuccessful, select <Cancel>.
NOTE: If <Cancel> is selected because the lock is
not functioning, troubleshoot the lock.
13. The prompt <Door Lock 2 (right) / Please close the main
door> displays. Select <OK>.
Elimination of Bubbles
1. To use the above FaWa Fill/Flush Protocol to eliminate
bubbles from the dispense tubing and syringes, deselect
the <Use Fast Wash Pump> checkbox (□), reduce the
<Fill Liquid Volume> from 30000 to 3000 and select <Fill
System>.
2. While the test is running, tap the liquid lines to dislodge
any bubbles lodged in the dispense tubing network.
7. Start the test with <Run>. The <Fast Wash Pump Test
Results> window opens.
10. Close the window with <Close>. The <Fast Wash Pump
Test Configuration> dialog is active again.
11. Finish with <Cancel>.
12. The test passed if the throughput value is greater than the
minimal throughput value displayed in the <Tip>
column. If the value under <Measured> is less than the
expected throughput value, the test failed.
13. If the test failed, verify tips are not clogged and recheck the
liquid system for air bubbles.
Perform Backup
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The EEPROM function allows backup of the EEPROM
parameters of one or more devices on disk. This also allows
parameters to be restored from disk. For example, performing a
backup of the current device parameters is recommended if an
electronic board must be replaced. Restoration of parameters
after the board is replaced eliminates redo of the setup and/or
calibration procedures.
Control Description
Do for Checkboxes to select the device addresses of
the EEPROMs to backup.
Device Name Indicates the subject device.
Restore with Displays the names of the files selected with
the <Browse […]> buttons.
Browse […] Command buttons that open the standard
Windows <Open> dialog to search the files
with parameters to restore.
EEPROM Backup
1. On the <Contents> page of the <Instrument>, <Basic
Setup> menu, select the <EEPROM Backup/Restore>
checkbox (聺) and switch to the <EEPROM> page.
LiHa Calibrations
LiHa calibrations are categorized by the implement used to
perform them. The <Calibrate Liquid Handler> menu offers
three calibration options based on those implements:
• Tip Adapter
• Reference Tip
• Disposable Tip
1 mL Subsystem Calibration
There are two stages to 1 mL Subsystem calibration. First,
heater zone 1, heater zone 2, the room temperature zone, and
the magnetic zone (MagLow) are calibrated, then using a 1 mL
Subsystem carrier, MagRest is calibrated.
15. Check the location values for X and Y. The X value should
be the same as MagLow X. The Y value should be 50 steps
greater than the MagLow Y value.
ASR Calibration
1. Mount the ASR Calibration tool on the assay reagent
holder.
Enabling a Rack
Sample tube size and style (spherical, conical, or flat bottom)
configurations must be set by an FSE at installation. Perform
the following steps to properly configure a system:
1. Navigate to <System>,<Settings>, and then <Sample
Rack and Tube>.
2. Select <Configure> from the <Setting Tasks> menu.
Disabling a Rack
To disable a previously enabled rack, deselect its check box
under <Rack Enable> and select <Yes> at the prompt.
RoMa Calibrations
The left-side <Calibrate Robotic Manipulator> menu offers
three calibration options:
• 1 mL Subsystem Calibration, found on page 4–158
• Disposable Tip Rack Calibration, found on page 4–161
• Shelf Calibration, found on page 4–165
1 mL Subsystem Calibration
For each calibration point, the RoMa gripper arms will be
properly aligned with the 1 mL Subsystem carrier.
IMPORTANT: It should NOT be necessary to
! adjust the Y-axis value during RoMa calibration.
Adjusting the Y value in one location might affect
the value at all locations. Therefore, if a Y change
is made in one location, the same change must be
made in all locations.
NOTE: The gripper open distance should be
adjusted minimally. These values will be less than
those seen in m2000sp Software Version 1.1 in
order to accommodate 96 sample processing.
1. Load a 1 mL Subsystem carrier with RVs, install the cover,
and place the carrier in the target area, beginning with
heater zone 1 and moving from location to location as
directed by the on-screen prompts.
The carrier must contain RVs so that deflection can be
observed if it occurs.
2. From the main menu select <System> then <System
Calibration>.
3. Select <1 mL Subsystem> from the <Calibrate Robotic
Manipulator> menu on the left side of the screen. The
<Robotic Manipulator Calibration> screen displays.
13. Use the arrows in the X and Y positions to align the RoMa
arms with the rack. The <Move> button will need to be
selected after each change to express the new coordinates
and to save them.
The goal is to have the RoMa gripper arms positioned just
below the lip on the sides of the carrier, and properly
centered on both sides. In the Y-axis, the gripper pins
should be equidistant to the sides of the DiTi rack and
should just touch the end of the rack.
Shelf Calibration
For each calibration point, the RoMa gripper arms will be
properly aligned with the shelf rack positions.
1. Load a 1000 µL DiTi rack on DiTi shelf 8. Push the rack in
until it just hits the rise at the rear of the shelf.
IMPORTANT: Ensure that the rack is NOT aligned
! with the front edge of the shelf.
2. From the main menu select <System> then <System
Calibration>.
3. Select <Shelves> from the <Calibrate Robotic
Manipulator> menu on the left side of the screen. The
<Robotic Manipulator Calibration> screen displays.
Calibration Verifications
M&D Procedures
To verify all LiHa and RoMa calibrations the following M&D
Procedures should be performed:
• Extensive Flush on page 5–10
• Dispense Test 50 µL, Gravimetric on page 5–23
• Dispense Test 800 µL, Gravimetric on page 5–26
• LiHa DiTi Location Test on page 5–12
• LiHa Sample Tube Check on page 5–14
• DiTi Rack Checks – Deck and Shelf
– RoMa DiTi Rack Test on page 5–27
– RoMa DiTi Shelf Test on page 5–29
• RoMa 1 mL Carrier Test on page 5–30
• Sample Extraction Test on page 5–37
• Z-Calibration Checks
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
All M&D procedures can be found in Section 5, Maintenance
and Diagnostic Procedures.
Network Configuration
Install the Router
IMPORTANT: For AbbottLink router installation, refer
! to the latest revision of the AbbottLink ISA.
Router Configuration
LAN ports 4 3 2 1
Crossover Cable
1. Enter “m2000rt-1” as the workgroup name.
2. Test the configured connection by selecting the <Test>
button.
3. Select <Save>.
3. Select <Save>.
On the m2000rt systems:
1. Navigate to the <System><Settings><Network
Configuration> screen.
Database Management
There are six backup options provided on the <Database
Management> menu:
• Full backup, which combines the System Database and
System Calibrations options, and includes all system data
• System Database only, which includes all database
information (including result information)
• System Calibrations only, which includes all data related
to LiHa and RoMa calibrations
• Configuration Files, which includes only system
configuration data
• System Logs, which include all log files except those from
Genesis
• Calibration Data, which only includes absolute
coordinates of previous calibrations
NOTE: All six categories can be backed up on a
CD; however, only the first three (Full, System
Database, and System Calibration) can be restored.
The last three options are backed up for
troubleshooting purposes only.
Figure 4.192:Backup
On-line Documentation
Load the on-line documentation after downloading all
necessary files from the GSS website’s Software Update page.
System Settings
To access the <System Settings> menu, select <System> then
<Settings>. This menu offers ten possible options:
• Hardware Configuration. Refer to Hardware
Configuration on page 4–44.
• Audible Alert
• Time and Date
• Sample Bar Code
• Sample Rack and Tube. Refer to Sample Rack and Tube
Configuration on page 4–149.
• Run Checks
• Signal Tower
• Language and Locale
• Network Configuration. Refer to Network Configuration
Screen on page 4–170.
• Automatic Export
Audible Alert
NOTE: The following procedure can also be performed
by customers.
This screen allows the user to:
• enable or disable the audible alert when an alert
dialog is generated
• choose a sound scheme
• adjust the volume for operator notifications
Dropdown menu
arrow
Run Checks
NOTE: The following procedure can also be performed
by customers with administrator access.
FSEs can enable or disable m2000sp run checks by selecting
<Settings> from the <System> menu, highlighting <Run
Checks>, and choosing <Configure> from the <Setting
Tasks> menu.
Signal Tower
NOTE: The following procedure can also be performed
by customers with administrator access.
The <Signal Tower> screen appears as an option on the
<Settings> menu for hooded instruments only.
The signal tower, which includes a red, a yellow, and a green
lamp, is designed to light up in a unique way for each of the
following five states:
• <Idle>: the instrument is ready
• <Running>: a run is in progress
• <Paused>: an instrument function was paused
• <Run Completed Successfully>: the run has finished
• <Run Failure>: the run has failed
2. Select <Save>.
3. Select <Save>.
To Change <Locale>
1. On the <Language and Locale> page, go to the <Locale>
field and click on the dropdown menu to select a regional
display preference.
2. Scroll down the list and make the selection that best fits
your preferences.
Network Configuration
Refer to Network Configuration on page 4–168.
Automatic Export
NOTE: The following procedure can also be performed
by customers with administrator access.
If a network or crossover cable connection was configured
during installation, customers with Administrator access can
choose to have Test Order results exported automatically on
the <Automatic Export> page.
1. To enable or disable automatic exporting, select
<Settings> from the <System> menu, highlight
<Automatic Export>, and choose <Configure> from the
<Setting Tasks> menu.
NOTES
SECTION 5:
MAINTENANCE AND
DIAGNOSTIC
PROCEDURES
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Pausing/Resuming/Stopping a Process . . . . . . . . . . . . . . 5-6
Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Flushes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Simple Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Extensive Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
LiHa Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
LiHa DiTi Location Test . . . . . . . . . . . . . . . . . . . . . . . . 5-12
LiHa Sample Tube Check . . . . . . . . . . . . . . . . . . . . . . . 5-14
LiHa Output Deck Test . . . . . . . . . . . . . . . . . . . . . . . . . 5-16
1 mL Subsystem Alignment Check . . . . . . . . . . . . . . . . 5-19
Dispense Test 50 µL, Visual . . . . . . . . . . . . . . . . . . . . . 5-21
Dispense Test 50 µL, Gravimetric . . . . . . . . . . . . . . . . . 5-23
Dispense Test 800 µL, Visual . . . . . . . . . . . . . . . . . . . . 5-24
Dispense Test 800 µL, Gravimetric . . . . . . . . . . . . . . . . 5-26
RoMa Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-27
RoMa DiTi Rack Test . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-27
RoMa DiTi Shelf Test . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-29
RoMa 1 mL Carrier Test . . . . . . . . . . . . . . . . . . . . . . . . . 5-30
System Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-32
LiHa Output Test - Sample Extraction . . . . . . . . . . . . . 5-32
LiHa Output Test - Master Mix . . . . . . . . . . . . . . . . . . . 5-35
Sample Extraction Test. . . . . . . . . . . . . . . . . . . . . . . . . . 5-37
Z-Calibration Check – Sample Extraction . . . . . . . . . . 5-40
Z-Calibration Check – Master Mix . . . . . . . . . . . . . . . . 5-45
RNA Simulation Test – Sample Extraction . . . . . . . . . . 5-47
RNA Simulation Test – Master Mix . . . . . . . . . . . . . . . . 5-50
Other Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-52
Chiller and Heater Temperature Check . . . . . . . . . . . . 5-52
Ethanol LLS Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-54
LiHa Sample Volume Checks . . . . . . . . . . . . . . . . . . . . 5-56
Overview
M&D procedures for use in troubleshooting and confirmation
of setup by FSEs are found under one of the following two
menus:
• Maintenance Procedures, found under the System menu
• Sample Extraction, found under the Orders menu
The following table indicates which M&D procedures are
found under which menu.
Table 5.1: M&D Menu Locations
Pausing/Resuming/Stopping a Process
To pause a process, select <Pause> or <Immediate Pause>
from the left-hand menu. At the prompt, select <Yes>.
Daily Maintenance
Flushes
Simple Flush
The <Simple Flush> purge might be required when:
• Isolated bubbles are found in the tubing between 3-way
valves and DiTi cones
• System sits idle for a few hours between runs
• Any LiHa component between the 3-way valve and DiTi
cone has been reassembled for replacement, calibration, or
check
Extensive Flush
The <Extensive Flush> purge might be required when:
• System is first set up
• When system fluid is refilled (outside of Daily
Maintenance)
• Any component within the fluid handling system has
been reassembled for maintenance, replacement,
calibration or check (e.g., replacing the system liquid)
• Special flush is performed using a cleaning solution
LiHa Checks
LiHa DiTi Location Test
Purpose
During this test, the LiHa will pick up and eject tips, allowing
the user to visually check the alignment of the DiTi cones with
the DiTis underneath.
Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips in each position
After the procedure:
• Adjust X and Y alignment in the DiTi racks and reuse racks
as needed
Procedure
1. Perform the following steps to set up the worktable:
• Load two 1000 µL DiTi racks with empty DiTi trays on
DiTi rack carrier 1
• Load two 200 µL DiTi racks with empty DiTi trays on
DiTi rack carrier 2
• Load empty DiTi trays and deep well plates in the DiTi
reuse racks on both DiTi rack carriers
• Fill column 1 and column 12 of all six DiTi trays with
DiTis in appropriate sizes, as shown below.
Purpose
During this test, the DiTi locations at the input sample tubes
will be visually verified.
Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips in each position: the
tips should be more or less centered in all tubes, with some
variability allowed as tubes sit differently in the racks
After the procedure:
• Adjust X and Y alignment in the input sample locations as
needed
Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Run all sample tube types being used by the customer,
but ensure that the sizes are not mixed on a rack (i.e.,
all 13 mm on one rack; all custom tubes on another
rack) and that the tube type matches the rack type
• Run all sample rack types being used by the customer
(13, 16, custom, or transport [pierceable])
• Run 16 tubes on each rack to check for run out from
front to back on the system
• Load reagent vessel carriers on the worktable (no bar
codes are needed)
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- LiHa Sample Tube Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
Purpose
During this test, the DiTi X-Y alignment at the assay reagent
bottles, master mix tube, deep well plate, and PCR plate on the
Output Deck will be visually verified.
Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips at all Output Deck
locations
After the procedure:
• Adjust X and Y alignment in the Output Deck locations as
needed
Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load an input sample rack with 8 tubes
• Load 8 reaction vessels in a 1 mL Subsystem carrier,
and place the carrier in the room temperature zone
• Load one reagent vessel containing a minimum of 20
mL of tap water or buffer solution into reagent vessel
position 6 on reagent vessel carrier 2 (the vessel
closest to the safety door) and place on the worktable
(no bar codes are needed)
• Load a clean, empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi carrier 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Load one empty reagent pack at position 1 (no bar
code needed)
• Load a clean master mix tube at position 1 on the
master mix tube holder
• Load a deep well plate onto the Output Deck
• Load a clean PCR plate on the chiller
13. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
14. Select <Orders>, <Master Mix Addition>, then in the
<Select a Plate> field, highlight the plate that was just
run. (This plate can be used repeatedly.)
15. Select <Set Up Run> from the <Master Mix Addition
Tasks> menu and perform the protocol as a typical Master
Mix Addition run.
16. Select <Next> on the <Master Mix Addition: Plate
Details> screen.
17. At the <Master Mix Addition: Worktable Setup> screen,
enter arbitrary numbers for the <PCR Plate Name> and
select <Next>. Ignore all on-screen instructions.
When the <Master Mix Addition: Disposable Tip
Status> screen appears, select <Next>.
18. On the <Master Mix Addition: Assay Specific Reagent
Scan> screen, select <Scan>, then <Next>.
19. Verify the master mix tube is in the correct location as
indicated on the <Master Mix Addition: Run Start>
screen and select <Start>.
NOTE: If a problem occurs and it becomes
necessary to rerun this test, it will NOT be
necessary to repeat the Sample Extraction portion.
20. The LiHa travels to various locations on the Output Deck,
diving into each location and pausing briefly. As it does,
visually ensure the DiTis are properly centered at each
location.
NOTE: If more time for visual inspection is
needed, select <Immediate Pause> from the
<Sample Extraction Tasks> menu while the LiHa
is paused. The safety door may be opened at this
time. Select <Resume> when the inspection is
complete.
21. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
22. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
Purpose
During this test, the DiTi X-Y alignment at the reaction vessels
on the 1 mL Subsystem will be visually verified.
Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips in each position,
verifying they are more or less centered throughout
• Check for positioning and alignment of the 1 mL
Subsystem carriers, ensuring they are set into position
evenly and with minimal deflection
After the procedure:
• Adjust calibration positions as needed
Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load input sample racks with a minimum of 16 tubes
• Load the same number of reaction vessels in a 1 mL
Subsystem carrier, beginning at position 1, and place
the carrier in the room temperature zone
• Load a clean, empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi carrier 1
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
– 1 mL Subsystem Alignment Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
Purpose
This small-volume test verifies the accuracy of fluidic
pipetting.
Actions
At the end of this procedure, be sure to:
• Visually inspect the RVs for a consistent level of fluid
• If inconsistencies are found, check the system fluidics for
leaks
Procedure
1. Set up the worktable as follows:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load input sample racks with 48 tubes, starting with
tube position 1
• Load the 1 mL Subsystem carrier with 48 clean
reaction vessels, beginning at position 1, and place
the carrier in the room temperature zone
• Load both reagent vessel carriers on the worktable (no
bar codes are needed)
• Fill the reagent vessel at location 6 (the vessel closest
to the safety door) with a minimum of 20 mL of tap
water or buffer solution.
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- 50 µL Dispense Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
Purpose
This small-volume test verifies the accuracy of fluidic
pipetting.
Actions
If inconsistencies are found at the end of this procedure, check
the system fluidics for leaks.
Procedure
All steps should be performed as outlined in the Dispense Test
50 µL, Visual, with the following changes:
1. Weigh each empty reaction vessel on a balance (accurate
to 0.5 milligrams or better) before loading it onto the 1 mL
Subsystem carrier during worktable setup. Number each
tube.
2. Weigh each reaction vessel from the 1 mL Subsystem
carrier at the end of the run using the same balance.
3. Calculate the volume transferred during the run by
subtracting the two values and then dividing that result by
the density of water (.000998 g/µL).
4. The gravimetric test passes if the mean value of the
calculated volume transferred is between 47.5 µL and 52.5
µL.
Purpose
This large-volume test verifies the accuracy of fluidic pipetting.
Actions
At the end of this procedure, be sure to:
• Visually inspect the RVs for a consistent level of fluid
• If inconsistencies are found, check the system fluidics for
leaks
Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load input sample racks with 48 tubes, starting with
tube position 1
• Load the 1 mL Subsystem carrier with 48 clean
reaction vessels, beginning at position 1, and place
the carrier in the room temperature zone
• Load reagent vessel carriers on the worktable (no bar
codes are needed)
• Fill the reagent vessel at location 6 (the vessel closest
to the safety door) with a minimum of 50 mL of tap
water or buffer solution
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- 800 µL Dispense Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Close the door and select <Scan>. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
Purpose
This large-volume test verifies the accuracy of fluidic pipetting.
Actions
If inconsistencies are found at the end of this procedure, check
the system fluidics for leaks.
Procedure
All steps should be performed as outlined in the Dispense Test
800 µL, Visual, with the following changes:
1. Weigh each empty reaction vessel on a balance (accurate
to 0.5 milligrams or better) before loading it onto the 1 mL
Subsystem carrier during worktable setup. Number each
tube.
2. Weigh each reaction vessel from the 1 mL Subsystem
carrier at the end of the run using the same balance.
3. Calculate the volume transferred during the run by
subtracting the two values and then dividing that result by
the density of water (.000998 g/µL).
4. The gravimetric test passes if the mean value of the
calculated volume transferred is between 760 µL and
840 µL.
RoMa Checks
RoMa DiTi Rack Test
Purpose
During this test, the RoMa will move the DiTi racks between
the DiTi rack carrier and the DiTi rack shelf.
Actions
As you run this procedure, be sure to:
• Check for positioning and alignment of the DiTi racks in
each position
• Verify that all movements are smooth, with minimal
contact between the racks and the surfaces
After the procedure:
• Adjust calibrations of the DiTi racks as needed
Procedure
1. Perform the following steps to set up the worktable:
• Load two 1000 µL DiTi racks on DiTi rack carrier 1 in
positions 1 and 2
• Load two 200 µL DiTi racks on DiTi rack carrier 2 in
positions 1 and 2
• Load 1000 µL DiTi racks in positions 2 through 8 on
the shelf
NOTE: For the purpose of this test, it does not
matter whether DiTis are present in the racks.
Purpose
During this test, the RoMa will move a DiTi rack to each of the
DiTi rack shelf's eight positions.
Actions
As you run this procedure, be sure to:
• Check for positioning and alignment of the DiTi rack in
each shelf
• Verify that all movements are smooth, with minimal
contact between the racks and the surfaces
After the procedure:
• Adjust calibrations of the DiTi racks as needed
Procedure
1. Perform the following steps to set up the worktable:
• Load one 1000 µL DiTi rack in shelf location 1
• Remove all other DiTi racks from the shelf
2. Select <System> then <Maintenance Procedures> or
select the Maintenance Procedure icon.
3. Select <RoMa DiTi Shelf Test> from the <Maintenance
Procedures> screen, and select <Set Up Run>.
4. Close the door and select <Start> to begin the test.
5. The RoMa will move the DiTi rack from shelf to shelf in
sequential order. At the end of each step, the RoMa will
return home.
6. If a problem is observed, select <Stop Process> to abort the
test.
7. The test will end automatically after checking all
locations.
8. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
9. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
Purpose
During this test, the RoMa will move two 1 mL Subsystem
carriers to all positions within the 1 mL Subsystem.
Actions
As you run this procedure, be sure to:
• Check for positioning and alignment of the 1 mL
Subsystem carriers in each position, ensuring they set into
position evenly and with minimal deflection of the RVs
• Check for interleaving of the carriers; no contact should
occur
After the procedure:
• Adjust calibration of the 1 mL Subsystem carriers as
needed
Procedure
1. Perform the following steps to set up the worktable:
• Load 96 empty reaction vessels into two 1 mL
Subsystem carriers (48 vessels per carrier)
• Lock the reaction vessels in place with the 1 mL
Subsystem carrier cover
• Place the first 1 mL Subsystem carrier in heater zone 1
and the second carrier in heater zone 2
2. Select <System> then <Maintenance Procedures> or
select the Maintenance Procedure icon.
3. Select <RoMa 1 mL Carrier Test> from the <Maintenance
Procedures> screen, and select <Set Up Run>.
4. Close the door and select <Start> to begin the test.
5. The RoMa will move the carriers to all positions on the 1
mL Subsystem. Observe the carrier movements and record
any problems found, looking for carrier placement
misalignment.
6. If a problem is observed, select <Stop Process> to abort the
test.
7. The test will end automatically after checking all
locations.
System Checks
LiHa Output Test - Sample Extraction
Purpose
This is the first portion of a buffer run procedure that enables
the user to evaluate m2000sp performance through a Sample
Extraction sequence, including transfer of eluate from the
reaction vessels to the deep well plate in the Output Deck. The
master mix process will be completed in the second portion of
the run.
NOTE: Two 995 reagent bar codes and reagent packs
will be needed for the master mix portion of this test.
Actions
At the end of the procedure, be sure to:
• Verify that no errors occurred (if any occur, the test has
failed)
• Examine the Process Logs for the minimum level sense
numbers:
– verify that the minimum level sense at MagRest minus
the calibrated bottom is greater than or equal to 7
– if this is not the case, check the calibration or the
system hardware
Procedure
1. Perform the following steps to set up the worktable:
• Load two full racks of 1000 µL DiTis on DiTi carrier 1
in locations 1 and 2
• Load input sample racks with 48 tubes
• Load 48 reaction vessels in a 1 mL Subsystem carrier,
and place the carrier in the room temperature zone
• Load one reagent vessel containing a minimum of 20
mL of tap water or buffer solution into reagent vessel
position 6 on reagent vessel carrier 2 (the vessel
closest to the safety door), and place the carrier on the
worktable (no bar codes are needed)
• Load a clean, empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi carrier 1
Purpose
This is the second portion of the buffer run procedure that
begins with the LiHa Output Test - Sample Extraction. This test
will take the plate created in the previous test and complete
the Master Mix Addition process.
NOTE: Two 995 reagent bar codes and reagent packs
will be needed for the master mix portion of this test.
Actions
As you run this procedure, be sure to:
• Pause after the master mix to PCR plate transfer to check
that all wells have an equal amount of fluid; if there is
inconsistency, check the system fluidics for leaks
After the procedure:
• Verify that all wells still have an equal amount of fluid
Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Beginning at position 1, load two reagent packs (with
reagent bar code 995) and reagent vials filled with tap
water in the volumes specified below:
Table 5.2: Tap Water Volumes
Purpose
During this procedure, a buffer run will be performed to
evaluate m2000sp performance through an entire Sample
Extraction sequence, except for eluate transfer from the
reaction vessels to the deep well plate.
NOTE: Reagent vessel RNA bar codes will be needed for
this test.
Actions
As you run this procedure, be sure to:
• Inspect the RVs following each liquid removal to verify
that all liquid was removed
• Check X and Y alignment of the tips—especially tip 3—in
each position, ensuring they are more or less centered in
all tubes
• Ensure that no water drops fall off the tips at the reagent
vessel locations during the system pause; if drops fall,
check the system fluidics for leaks
After the procedure:
• Adjust X and Y calibrations as needed
Procedure
1. Perform the following steps to set up the worktable:
• Load three full racks of 1000 µL DiTis on DiTi carrier
1, positions 1 and 2, and shelf position 2
• Load a clean empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi Carrier 1
• Load 13 mm sample racks with 48 reaction vessels,
filled with a minimum of 1 mL of tap water
• Load a 1 mL Subsystem carrier with 48 reaction vessels
and place the carrier in heater zone 1
Purpose
This is the first portion of a buffer run procedure. During this
test, Z-calibration at the input sample tubes, heater zones 1
and 2, magnetic rest, and the deep well plate locations will be
verified.
Actions
At the end of the procedure, be sure to:
• Verify that no errors occurred (if any occur, see
troubleshooting table below)
• Inspect the 1 mL Subsystem carrier to ensure that all liquid
was removed from the RVs
• Ensure the first 48 wells in the deep well plate contain an
equal amount of fluid: if there is inconsistency, check the
system fluidics for leaks
Table 5.4: Z-Calibration Troubleshooting
Procedure
1. Perform the following steps to set up the worktable:
• Load three full racks of 1000 µL DiTis on DiTi carrier
1, positions 1 and 2, and shelf location 2
• Load input sample racks with 48 tubes containing
740 µL of tap water each
• Load 48 reaction vessels in a 1 mL Subsystem carrier,
and place the carrier in heater zone 1
• Load one reagent vessel with a minimum of 30 mL tap
water onto reagent vessel position 6 (mElution™
Buffer site) on reagent vessel carrier 2 (no bar code is
needed).
• Load a clean DiTi tray and deep well plate onto the
DiTi reuse rack on DiTi rack carrier 1
• Load a clean deep well plate onto the Output Deck
14. Open the safety door and visually inspect the reaction
vessels in the 1 mL Subsystem. There should be minimal
liquid residue in the reaction vessels.
The deep well plate on the Output Deck should show a
uniform distribution of fluid over the first 48 wells; each
well should contain about the same amount of liquid. If
these conditions have been met, proceed with the Master
Mix Addition portion of this test.
Purpose
Actions
At the end of the procedure, be sure to:
• Verify that no errors occurred (if any occur, the test has
failed)
• Ensure the first 48 wells in the PCR plate contain an equal
amount of fluid: if the fluid level is inconsistent, check the
system fluidics for leaks
Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Beginning at position 1, load two reagent packs (no
bar code needed) and reagent vials filled with tap
water in the volumes specified below:
Table 5.5: Tap Water Volumes
Purpose
This is the first part of an RNA Simulation procedure in which
a buffer run will be performed to evaluate m2000sp
performance through an entire RNA extraction and master
mix sequence.
NOTE: Two 995 reagent bar codes and reagent packs,
and reagent vessel RNA bar codes, will be needed for
the master mix portion of this test.
In the Sample Extraction portion, all aspects of a typical RNA
run will be performed, except for duplicate washes or
incubation time.
Actions
As you run this procedure, be sure to:
• Examine each step of the process for correct calibrations
and adjust if necessary
• Examine each step of the process for correct liquid
handling: if fluid removals are not accurate, examine the
system fluidics for leaks
• Verify that no errors occurred during the run (if errors
occur, the test has failed)
After the procedure:
• Ensure the first 48 wells in the deep well plate contain an
equal amount of fluid: if there is inconsistency, check the
system fluidics for leaks
Procedure
1. Perform the following steps to set up the worktable:
• Load 13 mm sample racks with 48 tubes, containing a
minimum of 1.5 mL of tap water, starting with tube
position 1
• Load the 1 mL Subsystem carrier with 48 unused
reaction vessels, beginning at position 1, and place
the carrier in heater zone 1
• Load full 1000 µL DiTi racks on DiTi carrier 1,
positions 1 and 2, and in shelf positions 2-8
• Load a clean DiTi tray and deep well plate onto the
DiTi reuse rack on DiTi rack carrier 1
• Load a clean deep well plate onto the Output Deck
Purpose
This is the second portion of the buffer run procedure that
begins with the RNA Simulation Test – Sample Extraction. This
test will take the plate created in the previous test and
complete the master mix process.
NOTE: Two 995 reagent bar codes and reagent packs,
and reagent vessel RNA bar codes, will be needed for
this portion of this test.
Actions
As you run this procedure, be sure to:
• Examine each step of the process for correct calibrations
and adjust if necessary
• Examine each step of the process for correct liquid
handling: if fluid removals are not accurate, examine the
system fluidics for leaks
• Verify that no errors occurred during the run (if errors
occur, the test has failed)
After the procedure:
• Ensure the first 48 wells in the PCR plate contain an equal
amount of fluid: if there is inconsistency, check the system
fluidics for leaks
Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Beginning at position 1, load two reagent packs (with
reagent bar code 995) and reagent vials filled with tap
water in the volumes specified in the table below
Table 5.7: Tap Water Volumes
Other Checks
Chiller and Heater Temperature Check
Purpose
During this test, the set temperatures at heater zone 1, heater
zone 2, and the PCR tray holder will be confirmed.
Actions
If either heater zone fails to come to temperature:
• Check the cabling at the temperature block; check the
tightness of the connections
• Check the heater block hardware (heater boards, etc.)
If the chiller fails to reach temperature:
• Check the cabling at the chiller
• Check the chiller hardware
Procedure
1. Perform the following steps to set up the worktable:
• Load a reaction vessel with 1 mL of water in column
3, position 4 of the 1 mL Subsystem carrier and install
the carrier cover
• Place the 1 mL carrier in heater zone 1
2. Select <System> then <Maintenance Procedures> or
select the Maintenance Procedure icon.
3. Select <Chiller and Heater Temperature Check> from
the <Maintenance Procedures> screen and then select
<Set Up Run> from the <Maintenance Tasks> menu on
the left.
4. Close the door and select <Start> to begin the test. The
chiller temperature will begin to descend, and the system
will automatically pause, allowing the operator to open
the door.
5. Set the TVK into the PCR plate holder on the chiller. The
TVK should remain there throughout the test to allow
temperature readings to be taken periodically over the
next 20 minutes.
Purpose
During this buffer run procedure, liquid level sensing is
performed six times for mWash 2DNA.
Actions
If level sensing fails:
• Check that the correct amount of fluid was added to the
trough
• Check the connections for the level sensing circuit (ILID
board, ILID cable, tip adapter)
• Check the hardware for the level sensing circuit (ILID
board, ILID cable, tip adapter)
• Retry the test
Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load a 13 mm input sample rack with 8 tubes
• Load one reagent vessel with a minimum 30 mL of
70% Ethanol solution into reagent vessel position 5
(no bar code is needed)
2. Select <Orders>, <Sample Extraction>, then <Ethanol
LLS Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
At the <Sample Extraction: Worktable Setup> screen,
ignore all instructions and select <Next>.
Purpose
These checks determine the minimum sample volume needed
for a given tube and a given sample aspiration volume in order
to achieve reliable aspiration.
There are four sample volumes that can be checked:
• 200 µL
• 400 µL
• 500 µL
• 600 µL
• 1000 µL
These checks are particularly useful for determining the
minimum sample volume required in custom tubes. Refer to
Sample Rack and Tube Configuration on page 4–149 of Section
4, Installation, for more information on custom tubes.
Actions
The test should be considered a failure after two unsuccessful
100 µL water additions.
If failure occurs:
• Ensure that tap water, and NOT deionized or distilled
water, was used
• Check the connections for the level sensing circuit (ILID
board, ILID cable, tip adapter)
• Check the hardware for the level sensing circuit (ILID
board, ILID cable, tip adapter)
Procedure
1. Perform the following steps to set up the worktable:
• Prepare 13 or 16 mm sample racks with a minimum of
8 tubes, starting with tube position 1. Fill each tube
with tap water in the sample volume desired (200,
400, 500, 600, or 1000 µl) plus an additional 200 µL
• Load the sample racks on the worktable
SECTION 6:
UPGRADE PROCEDURES
NOTES
Table of Contents
Procedural Resources
Hood Installation
Hoods are not standard equipment on all G-series instruments.
When installation is required, refer to the latest revision of TSB
605-018.
A warning sticker
shows when the
waste chute is
being installed
incorrectly.
A green circle
appears on the
front of the waste
chute.
9. Select OK.
NOTE: If the calibration fails, check the
connection to the SMIO board and repeat the test.
NOTE: If the calibration fails again, replace the
sensor.
10. Perform the DiTi Waste Sensor Verification.
SECTION 7:
TROUBLESHOOTING
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-5
Error Codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
Process Management Issues (Error Codes 1000-1099) . . 7-6
System Calibration Issues (Error Codes 1100-1199) . . . . 7-6
Test Order Issues (Error Codes 1200-1299) . . . . . . . . . . . 7-6
Reagent Management (Error Codes 1300-1399) . . . . . . . 7-7
Check Configuration (Error Codes 1500-1599) . . . . . . . 7-7
Signal Tower (Error Codes 1600-1699) . . . . . . . . . . . . . . 7-8
LiHa Calibration (Error Codes 2000-2099) . . . . . . . . . . . 7-8
RoMa Calibration (Error Codes 2100-2199) . . . . . . . . . . 7-8
Run Application (Error Codes 2200-2299) . . . . . . . . . . . 7-8
Run Master Mix Addition (Error Codes 2300-2379) . . . 7-8
Hardware Configuration (Error Codes 2380-2399) . . . . 7-9
Shared Sample Preparation (Error Codes 2400-2499) . . 7-9
Instrument Control (Error Codes 2500-2599) . . . . . . . . 7-9
Sample Preparation System Calibrations (Error Codes
2601-2699) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-10
Sample Preparation Command Library (Error Codes
2700-2799) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-11
Sample Preparation Data Server (Error Codes 2800-2899)
Robotic Sample Processor (RSP) COM (Error Codes
3040-3099) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-13
Liquid Handler (LiHa) (Error Codes 3101-3199) . . . . . 7-13
Dilutor (Error Codes 3201-3299) . . . . . . . . . . . . . . . . . 7-22
Robotic Manipulator (RoMa) (Error Codes 3301-3399) 7-23
Bar Code Scanner (PosID) (Error Codes 3401-3499) . . 7-25
Doorlocks (Error Codes 3502-3599) . . . . . . . . . . . . . . . 7-26
Fast Wash Pump (FaWa) (Error Codes 3602-3699) . . . . 7-27
Heaters (Error Codes 3701-3799). . . . . . . . . . . . . . . . . . 7-27
SMIO (Smart Monitored Input/Output) (Error Codes
3802-3899) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-27
Output Deck Chiller (Error Codes 3900-3949) . . . . . . . 7-28
Hood (Error Codes 3950-3999) . . . . . . . . . . . . . . . . . . . 7-29
Disposable Tip Inventory (Error Codes 7000-7099) . . . 7-29
User Notification (Error Codes 7100-7199) . . . . . . . . . 7-29
Application Specification (Error Codes 7200-7299) . . . 7-29
Result Management (Error Codes 7300-7399) . . . . . . . 7-30
Backup and Restore (Error Codes 7400-7499 / 8700-8799) .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-30
Shared Application Specification (Error Codes 7600-7749) .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-31
Network Data Store (Error Codes 7750-7799) . . . . . . . 7-31
Error Codes
• Check that all racks and carriers are in the same position
as during the initial sample scan
• Verify carriers and racks are enabled
• Check that all bar codes meet specifications and are
configured correctly
General Errors
• Cycle power on the SCC and the instrument
The majority of these errors codes are user notification
prompts. Follow the instructions given on the prompts.
Sensor Errors
• Check full/empty status of the indicated sensor
• Cycle power on the SCC and the instrument
DiTi Errors
• Check that all DiTi cans are loaded in the correct location
• Update the disposable tip inventory status (navigate to
<System>,<Disposable Tip Status>)
NOTE: Refer to Calibrate Disposable Tip Locations
on page 4–153.
Heater/Chiller Errors
• Cycle power on the SCC and the instrument
• Cycle power on the chiller
• Unplug and replug the Edgeport™
• Check cable connections
• CheckHeater and CheckChiller turned off errors are due to
Application Specification problems; contact your local FAS
• If runs are performed back-to-back, the heater might not
be able to cool down to the start temperature fast enough
• If you suspect a hardware issue, refer to Heater Hardware
on page 7–38
Process/Command Aborted
• Check the logs and troubleshoot devices as appropriate
• Cycle power to the instrument
• Check that communication with the instrument has been
established
• If error occurred in the master mix
– check for sufficient fluid in the reagent pack and
master mix tube
– check for bubbles in the reagent pack
• If error occurred during extraction, check that the 1 mL
Subsystem carrier or carriers have been placed correctly
• Check that all commodities have been placed correctly
• Rerun the samples if possible
Initialization Error
• Check for obstructions to the LiHa
• Cycle power to the instrument and reinitialize
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
• If error occurred during extraction, ensure that adequate
sample volume (per package insert) is present
Tip Errors
• Verify that tips are available
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance
NOTE: There should be no attempt to retry at
the trough or heater zone 1.
• Log Troubleshooting
– Check the run logs (Process Log, Message History Log,
Process.LiHa.log) to see if a specific tip adapter has
been having consistent level sense errors, which
might indicate a hardware failure of that tip
adapter/ILID cable/ILID board
• Sample Troubleshooting
– Check liquid for clots
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
– Check that the sample was vortexed/centrifuged
correctly
• Hardware Troubleshooting
– Check tip adapters
• Sample Troubleshooting
– Check for proper volumes in reagent vessels and input
samples
– Check liquid for clots
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check all liquid system connections
– Inspect system liquid tubing
– Check the syringes, valves, and dilutors
– Check for large bubbles forming on the top of the
syringe
– Check the integrity of the fluidics system
NOTE: If the problem is specifically noted on tips
1-4 or 5-8, check the 1:4 manifolds on the top of
the system.
• Instrument Troubleshooting
– Check the calibrations at the problem locations
• Sample Troubleshooting
– Check liquid for clots
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check all liquid system connections
– Inspect system liquid tubing
– Check the syringes, valves, and dilutors
– Check for large bubbles forming on the top of the
syringe
– Check the integrity of the fluidics system
NOTE: If the problem is specifically noted on tips
1-4 or 5-8, check the 1:4 manifolds on the top of
the system.
• Instrument Troubleshooting
– Check the calibrations at the problem locations
– Check the instrument Y-spread
• S&S troubleshooting
– Perform a liquid detection test, increasing the clot
detection distance to 40
NOTE: If the test fails, check the Z-rods for
excessive hysteresis.
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance: after each fluid transfer,
pause to check for correct volumes; check for correct
DiTi centering throughout the test
• Log Troubleshooting
– Match failures in the logs (Process Log,
process.liha.log) with hardware failures
– Note the failure positions and volume trends (additive
failures) from previous steps
• Sample Troubleshooting
– Check for sufficient volume in sample tubes
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check tip adapters
– Check ILID cable connections
– Check ILID cables
– Check ILID board
– Examine the 1 mL Subsystem carrier plate for
deformity
• Instrument Troubleshooting
– Check the calibrations at the problem locations
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
• Sample Troubleshooting
– Check for sufficient volume in sample tubes
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check tip adapters
– Check ILID cable connections
– Check ILID cables
– Check ILID board
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Plunger/Valve Overload
• Cycle power to the instrument
• For a plunger overload, replace the failed syringe, ensuring
it is properly seated in the thumb screw (refer to the logs if
the failed plunger cannot easily be seen)
• For a valve overload, replace the failed valve
• Replace the failed dilutor if necessary
• If you suspect a hardware issue, refer to Dilutor Hardware
on page 7–34
Initialization Error
• Check for obstructions to the RoMa
• Cycle power to the instrument and reinitialize
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Initialization Error
• Check for obstructions to the PosID
• Remove extra carriers at the end of the worktable
• Cycle power to the instrument and reinitialize
• Check frogs for wear
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
Additional Errors
• Check cable connections at the SMIO board and at the
SCC
• If you suspect a hardware issue, refer to SMIO/SMIO-SAFY
Hardware on page 7–58
Communication Errors
• Check the seating of the power cable at the chiller
• Check the Edgeport™ cable connections
• Cycle power on the chiller
• Unplug and replug the Edgeport
• If you suspect a hardware issue, refer to Output Deck
Hardware on page 7–44
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37
Temperature Errors
• Check all cable connections between the chiller and the
m2000sp instrument
• Check the Edgeport cable connections
• Cycle power on the chiller
• Unplug and replug the Edgeport
• Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• If you suspect a hardware issue, refer to Output Deck
Hardware on page 7–44
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37
Incompatible Hardware
Use the hardware configuration screen to reconfigure a
G-Series as an E-Series.
Signal Tower
• Check cable connections
• Cycle power to SCC and instrument
• Verify that the signal tower is implemented in the
Hardware Configuration screen
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37 or Signal Tower Hardware on page
7–59
General Errors
For most result management errors, you will need to reprocess
the samples.
Exporting Errors
• Try exporting the Test Order files again
• Retry export with a different CD
• “Network” might not be available as an export option;
configure m2000rt computer name and check cabling
• Check the network cable at the m2000rt™ and m2000sp
• Cycle power on the SCC
• Replace the CD
• If you suspect a hardware issue, refer to SCC Hardware on
page 7–54
Backup/Restore Failed
• Open/close CD drive door, wait 12 seconds, and try again
• Replace the CD
• Cycle power on the SCC
• Retry the procedure
• Check the logs for information
• Ensure that all non-application windows are closed
behind the application window
• If you suspect a hardware issue, refer to SCC Hardware on
page 7–54
Database Failure
First, retry the procedure. The database that is being backed
up/restored might be incompatible with the current software
version. Contact GSS to see if there is any way the database
can be read.
The majority of these error codes relate to actions during
processing (completion of backup, invalid state, etc.). Follow
any necessary prompts for these actions.
Test Failed
• Check crossover cable connections at router and SCC
• Check power to components (SCC and router)
• Check cable type
• If you suspect a hardware issue, refer to Network Hardware
on page 7–59
• If you suspect a configuration issue, refer to Network
Configuration Screen on page 4–170 of Section 4
Hardware Errors
In S&S:
• Verify that under <Options>,<SMIO Panel
Configuration type>, the device is listed as NAT systems
• Check the information panel to ensure that all devices are
available (<Instrument>,<Basic Set-up>,<Information>)
Dilutor Hardware
Doorlocks Hardware
Edgeport™ Hardware
In Explorer:
• Verify that Port 1 is set to COM3 in the Edgeport
Configuration menu
FaWa Hardware
In S&S:
• Perform FaWa Fill/Flush Protocol on page 4–122 of Section
4
• Perform FaWa Pump Test on page 4–123 of Section 4
• OPTIBO board
Heater Hardware
LiHa Hardware
No Response
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Verify the Device M1 is known on the Firmware
Download page (if not, download LiHa firmware)
– Enter the single line command A1REE
– The response should be A1@@@@@@@@@@@ if
all axes are responding correctly
– If errors are received, address the specific axis
involved by following the steps in the
appropriate section below
• Check the following and replace if necessary:
– Indicator light on Genesis CU board (if out, the board
needs to be replaced)
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Cable connection between the X-drive assembly and
the LiHa backplane board
– Fuse FU2 on the OPTIBO board
– Cables
– OPTIBO board
– LiHa backplane board
LiHa X-axis
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Enter the single line command A1REE
– The response should be A1N@@@@@@@@@@,
where N is the axis error code
– Check Y-spread
– Check LiHa reference positions
– Check instrument scaling
• Check the following and replace if necessary:
– Look for obstructions that would prevent proper
initialization of the LiHa
– Fuse FU2 on the OPTIBO board
– Cable connection between the X-drive assembly and
the LiHa backplane board
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Verify the distance between the X-drive and the X-gap
is 2.0 mm
– X-drive belt
– X-drive assembly
– Cables
– Servo power board
– Device CU board
– LiHa backplane board
– LiHa
LiHa Z-axis
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
DiTi Ejection
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Check Each of the Tip Adapters on page 4–58 of
Section 4
– Calibrate the Lower DiTi Eject on page 4–63
– Perform the Lower DiTi Eject Test on page 4–64
Tip Adapters
• In S&S:
– Perform the Tip Adapter Test (refer to Check Each of
the Tip Adapters on page 4–58)
• Check the following and replace if necessary:
– The straightness and alignment of the tip adapter
– The set screw on the Z-rod
Chiller
• In m2000sp Application:
– Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• Check the following and replace if necessary:
– Power switch on the back of the chiller is ON
– Power cord is firmly plugged into the back of the
chiller
– The RS-232 cable between the chiller and the
Edgeport™ is connected to Port 1 on the Edgeport
PosID Hardware
Tube Sensing
• Check the following and replace if necessary
– Clean the tube sensors
– Cable connection between the PosID CU board and
PosID board
– Cable connection between the OPTIBO board (CAN
Opt 2-CU PosID) and the PosID CU board
– Cable connection of the X-flex cable between the
PosID board and PosID 2 module
– Cable connection between the NO Tube Sensor and
the Y/B board
– No Tube Sensor
– Y/B board
– Servo board
– Y/B cable
– X-flex cable
– PosID CU board
PosID 2 X Movement
• In S&S:
– Enter the single line command P1REE
– The response should be N@@, where N is the
axis error code
• Check the following and replace if necessary
– Manually check for free movement of the PosID 2
– Cable connection between the X-motor and PosID
board
– Cable connection between the PosID CU board and
the PosID board
– Check the X-belt tension
– Cable connection of the X-flex cable between the
PosID board and PosID 2 module (Y/B board)
– Cable connection between the OPTIBO board and the
PosID CU board (CAN Opt 2 – PosID)
– Fuse FU7 on the OPTIBO board
– Cables
– X-servo board
– X-motor
– X-drive assembly
– Belt
– PosID board
– OPTIBO board
PosID 2 Y-movement
• In S&S:
– Enter the single line command P1REE
Power Supply
Printer Hardware
RoMa Hardware
No Response
• In S&S:
– Verify that the RoMa is recognized on the Basic Setup
Information tab
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Verify that Device C1 is known on the Firmware
Download page (if not, download RoMa firmware)
– Enter the single line command C1REE
– The response should be C1@@@@@ if all axes
are responding correctly
RoMa Rotator
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Verify that Device C1 is known on the Firmware
Download page (if not, download RoMa firmware)
– Enter the single line command C1REE
– The response should be @@@N@, where N is
the rotator error code
– Perform the Reference Plate Test on page 4–79 of
Section 4, and auto-adjust the rotator if necessary
– After reperforming the Reference Plate Test, perform
Manual RoMa Rotator Adjustments on page 4–83 if
necessary
• Check the following and replace if necessary
– Manually rotate the gripper head, and check for
bearing roughness
– Cable connection between the rotator motor and
gripper head
– Cable connection between the gripper board and the
RoMa 2 backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Common servo boards (swap to check for board
failures); check board address settings
– Rotator motor
– Y/R servo board
– RoMa 2 backplane board
– Gripper board
– RoMa Freedom backplane board
• In m2000sp Application:
Run any of the RoMa alignment M&D checks to verify
correction rotational alignment of the RoMa (refer to
RoMa Checks on page 5–27 of Section 5). Manually align
the RoMa rotator if necessary.
RoMa Gripper
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Enter the single line command C1REE
– The response should be @@@@N, where N is
the gripper error code
– Check the gripper setup in <RoMa>,<Set Type>
– Perform the Reference Plate Test on page 4–79 of
Section 4, and auto-adjust the rotator if necessary
• Check the following and replace if necessary
– Manually rotate the gripper head, and check for
bearing roughness
– Use the move panel to move the gripper fingers above
a straight surface (the reference teach plate, for
example) to verify the fingers are parallel and even
– Use the move panel to move the gripper fingers above
the 1 mL Subsystem and bring them down to the
surface to verify that the bottoms of the pads are flat
to the surface; readjust the screws if necessary
– Cable connection between the gripper motor and
gripper board
– Cable connection between the gripper board and the
RoMa 2 backplane board
RoMa Y-axis
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Enter the single line command C1REE
– The response should be @N@@@, where N is
the Y-axis error code
– Check the scaling factor
– Initialize the axis and check for extended errors
• Check the following and replace if necessary
– Manually check for free movement of the RoMa arm
– Y-belt tension
– Screws between the axles and the pulley
– Cable connection between the Y-pulley and the
RoMa 2 backplane board
– Cable connection between the RoMa 2 backplane
board and the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Y-belt
– Servo board
– Y-motor
– Cables
– RoMa 2 backplane board
– RoMa Freedom backplane board
– RoMa
RoMa Z-axis
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Enter the single line command C1REE
– The response should be @@N@@, where N is
the Z-axis error code
– Check the RoMa range
– Initialize the Z-axis and check for extended errors
• Check the following and replace if necessary
– Manually check for free movement of the RoMa arm
– Grease the worm gear
– Cable connection between the Z-motor and the
RoMa 2 backplane board
– Cable connection between the RoMa 2 backplane
board and the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Z-motor
– Servo board
– Cables
– RoMa 2 backplane board
– RoMa Freedom backplane board
– RoMa
RoMa X-axis
• In S&S:
RoMa Z-brake
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
• Check the following and replace if necessary
– Clean the Z-brake
– Check the Z-brake for cracks
– Verify the distance between the brake shoe and
Z-brake is 7.25 mm +0.2mm; adjust if necessary
SCC Hardware
Communication Errors
• Check the following and replace if necessary
– Cable connection between the 1 mL Subsystem and
CAN options 3 and 4
– Fuse FU1 on the OPTIBO board
– Cable connection between SCC and the OPTIBO
board (Host RS-232)
– Check that the port setting is set to COM1
– RS-232 cable
– Power cable
– OPTIBO board
– CU board
• After a short-term m2000sp shutdown, cycle SCC power
No Display
• Check monitor connections to SCC
• Check that power switches are in the on position (one in
front and one in rear next to power cord)
Long Bootup
It is normal for networked systems to take an additional 60
seconds to bootup as they acquire the network.
Memory Problems
• If the RAM buffer is out of available memory, restart the
SCC to clear the buffer
Sensor Hardware
Work through the following Problem Solving Tree to resolve
sensor problems:
SMIO/SMIO-SAFY Hardware
In S&S:
• Check board function using <Options>,<SMIO for NAT>
and <Options>,<Access Status Options>
Database/Restore Issues
• Shutdown the SCC (particularly if you observe a red X or
system lock up)
• Restart the SCC
• Login as FSE
• Navigate to the Database Manager
• Place the Backup CD in the drive
• Wait until the CD light turns off (approximately
12 seconds)
• Select restore
Network Hardware
In m2000sp Application:
• Check that the network is correctly configured
Check the following and replace if necessary:
• Network cable at router is connected to LAN jack
• Network Data Store computer is connected to LAN jack
• Router should be powered on and blinking green
• SCC is connected to router
NOTES
SECTION 8:
MAINTENANCE
NOTES
Table of Contents
TOC
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-5
Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-6
Cleaning Agents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-6
Clean Liquid System Procedures . . . . . . . . . . . . . . . . . . . . . 8-8
Microbial Growth Inspection . . . . . . . . . . . . . . . . . . . . . 8-8
Clean the System Liquid Container Procedure . . . . . . . 8-8
ProClin® Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-10
Clean the Liquid Waste Container . . . . . . . . . . . . . . . . 8-10
RoboScrub Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . 8-12
Gas Spring Maintenance Check . . . . . . . . . . . . . . . . . . . . . 8-14
Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-15
At Each Site Visit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-15
Biannual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-15
Annual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-17
m2000sp G-series Biannual Preventive Maintenance Checklist
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-19
m2000sp G-series Annual Preventive Maintenance Checklist . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-21
Introduction
This section summarizes the biannual and annual preventive
maintenance procedures. Maintenance must be performed as
described in this manual. For optimum performance and
reliability of the instrument, routinely perform the
maintenance and cleaning tasks described in this chapter.
IMPORTANT: Be sure to verify the instrument you are
! maintaining is a G-series and not an E-series model.
(For E-series model maintenance information, see the
m2000sp E-series Service Manual.)
Safety
Observe the following safety notices.
Consumables
Detailed cleaning instructions are located in Section 8, Service
and Maintenance of the m2000sp Operations Manual, which
is available for review within the m2000sp Software under the
<Help> menu.
Cleaning Agents
Alcohol
Use ethanol or 2-Propanol (Isopropanol).
Bleach
Use 0.1% or 0.5% sodium hypochlorite solution (2% or
10% chlorine bleach) depending on the task to be performed.
WARNING: Do not mix any oxidizing agents such as
straight household bleach, or any solution that
contains sodium hypochlorite in percentages higher
than 0.5%, with the mLysis, mWash 1, mMicroparticles,
mLysisDNA, mWash 1DNA, or the mMicroparticlesDNA
reagents in the Abbott sample preparation reagent
pack. Do not mix any oxidizing agents such as sodium
hypochlorite with the liquid waste of the m2000sp.
Toxic gases could be generated from these mixtures.
Pressure can build up in the closed container of the
mixtures. It is the responsibility of each facility to
characterize its waste and ensure it is handled and
disposed of in accordance with local, state, and federal
regulations.
Water
Use distilled or deionized water.
CAUTION: Strong detergents may dissolve carrier and
worktable surface coatings.
• Use only recommended cleaning agents.
• Chlorine solution can pit equipment and metal.
Use adequate amounts or repeated applications of
70% ethanol or water until chlorine residue is no
longer visible.
Detergent Solutions
• Sporicidin Brand Disinfectant Solution (pump spray and
refill) EPA Reg.: 8383-3; Sporicidin International,
Rockville, MD
• Sporicidin Brand Disinfectant Towelette EPA Reg.: 8383-7;
Sporicidin International, Rockville, MD
• RoboScrub: contact Abbott Molecular GSS for assistance in
ordering RoboScrub.
• ProClin® 300 Preservative (product number 48914-U)
can be purchased globally from the Sigma-Aldrich
company’s website at www.sigmaaldrich.com
ProClin® Procedure
1. Remove the cap from the system liquid container, empty
whatever liquid remains, and refill the container with 2.5
liters of distilled or deionized water.
2. Volumetrically add 3.5 mL of ProClin® 300 to the water in
the container.
CAUTION: Chemical Hazard. ProClin 300
Preservative is a corrosive chemical. Refer to its
MSDS, laboratory guidelines, and local
government regulations for instructions on proper
handling and disposal. Wear appropriate personal
protective equipment such as gloves, lab coats,
and protective eyewear.
NOTE: Serological pipettes or any other
volumetric measurement device with an accuracy
better than + 0.2 mL can be used.
3. Fill the container with an additional 7.5 liters of distilled
or deionized water (for a total of 10 liters).
RoboScrub Procedure
This procedure purges the system liquid pathway, including
tubing, valves, pumps and connectors with a detergent
solution (1.5% solution of RoboScrub). It should be performed
only at the recommendation of Abbott Molecular GSS.
WARNING: Potential Biohazard. Follow basic
biohazard precautions. Appropriate personal protective
equipment should be worn during all cleaning and
assay procedures.
10 inches
Maintenance Schedule
At Each Site Visit
• Perform Microbial Growth Inspection on page 8–8
• Print system information (refer to Information on page
11–57 of Section 11)
• Run counter readout (refer to the individual device’s
Information page within S&S)
Biannual
Refer to the m2000sp G-series Biannual Preventive
Maintenance Checklist on page 8–19 for a complete listing of
biannual maintenance procedures.
This section discusses special conditions FSEs need to be aware
of and provides a general listing of where specific checklist
procedures or tests can be found in this manual.
Parts to be Replaced
There are two different DiTi Spare Sets available depending on
your instrument’s DiTi O-ring configuration. If you plan to
upgrade to the new DiTi/O-ring configuration, refer to TSB
605-008 for directions. Otherwise, be sure to order the
appropriate part. Refer to DiTi Adapter (DiTi Spare Set)
Replacement on page 9–63 of Section 9 and the part numbers
listed in Section 12.
Parts to be Cleaned
• The following items can be cleaned with alcohol and a
lint-free cloth:
– Z-drive rod and guide rods for RoMa
– Z-drive rod for LiHa
– Arm guide
– Lower DiTi Eject linkage and guide rods
• To clean the Y-square bar, use a lint-free cloth (do not use
any agent). Remove side covers of LiHa as described in
Complete LiHa Assembly on page 9–40 of Section 9.
Lubrication
Part number 10626104 (see Section 12 for details) consists of:
• One tube of food-grade lubricant grease, which is used for:
– the RoMa guide rods
– the Lower DiTi Eject Pivot Pin
• One tube of Lubricant grease, which is used for:
– the LiHa, RoMa, and PosID X-slide shafts
IMPORTANT: Never use a spray-on lubricant.
!
NOTE: Lubricant should be applied sparingly and only
when necessary.
Annual
Refer to the m2000sp G-series Annual Preventive Maintenance
Checklist on page 8–21 for a complete listing of annual
maintenance procedures.
This section discusses special conditions FSEs need to be aware
of and provides a general listing of where specific checklist
procedures or tests can be found in this manual.
Parts to be Replaced
• DiTi Spare Sets: Be sure to order the appropriate part for
your O-ring configuration. Refer to DiTi Adapter (DiTi
Spare Set) Replacement on page 9–63 of Section 9.
• 3-way Valves: Be sure to order the appropriate valve for
your dilutor configuration.
– For any new XP Smart Dilutors, order valve 10649016
– For any XP3000 Dilutors, order valve 04J97-42
Parts to be Cleaned
• The following items can be cleaned with alcohol and a
lint-free cloth:
– Z-drive rod and guide rods for RoMa
– Z-drive rod for LiHa
– Arm guide
– Lower DiTi Eject linkage and guide rods
• To clean the Y-square bar, use a lint-free cloth (do not use
any agent). Remove side covers of LiHa as described in
Complete LiHa Assembly on page 9–40 of Section 9.
• To clean the system liquid assembly, refer to Clean the
System Liquid Container Procedure on page 8–8.
• To clean the liquid waste assembly, refer to Clean the
Liquid Waste Container on page 8–10.
Lubrication
Part number 10626104 (see Section 12 for details) consists of:
• One tube of food-grade lubricant grease, which is used for:
– the RoMa guide rods
– the Lower DiTi Eject Pivot Pin
• One tube of Lubricant grease, which is used for:
– the LiHa, RoMa, and PosID X-slide shafts
IMPORTANT: Never use a spray-on lubricant.
!
NOTE: Lubricant should be applied sparingly and only
when necessary.
Maintenance Procedures
Done/
Action Item N/A Failed Comment
Passed
Diti cones, Tubing
extensions, O-rings
Replace
Any missing or damaged
worktable frogs
Arm guide
Done/
Action Item N/A Failed Comment
Passed
Lower DiTi Eject pivot pin
Lubricate
RoMa guide rods
(see page
8–16) X-slides for PosID, RoMa,
and LiHa
Z-Calibration Test
(Sample Extraction)
Additional Comments
Signatures
Maintenance Procedures
Done/
Action Item N/A Failed Comment
Passed
Syringes
3-way valves
1:4 manifolds
Pipetting tubing
Interconnecting tubing
FaWa tubing
Clamps
Y-distributors
Syringes
Done/
Action Item N/A Failed Comment
Passed
Arm guide
Done/
Action Item N/A Failed Comment
Passed
Tip Adapter Test
Fill/Flush FaWa
Additional Comments
Signatures
SECTION 9:
REPAIR, REPLACEMENT
OF SPARE PARTS
NOTES
Table of Contents
TOC
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-6
Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Required Special Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Removal of Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Installation of Worktable . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
System Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11
Spare Parts Discussed in This Section . . . . . . . . . . . . . . 9-11
OPTIBO Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-12
Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-17
CU Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-17
DC-DC Power Board . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-21
SMIO and SMIO-SAFY Board . . . . . . . . . . . . . . . . . . . . 9-24
Power Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-29
Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-29
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-31
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-31
X-Drive Assembly and X-Belt (for LiHa and RoMa) . . . . . . 9-32
Spare Parts X-drive Assembly . . . . . . . . . . . . . . . . . . . . 9-32
X-drive Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-32
X-drive Belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-36
Liquid Handling Arm (LiHa) . . . . . . . . . . . . . . . . . . . . . . . . 9-39
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-39
Complete LiHa Assembly. . . . . . . . . . . . . . . . . . . . . . . . 9-40
Electronic Boards and Cables . . . . . . . . . . . . . . . . . . . . 9-43
Y-belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-52
Y-motors (Y-DC-Servo or Y-spreading). . . . . . . . . . . . . . 9-56
DiTi Mechanism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-63
DiTi Adapter (DiTi Spare Set) Replacement . . . . . . . . . 9-63
White Separator Ring and Black O-ring Assembly . . . . 9-67
Lower DiTi Eject Option . . . . . . . . . . . . . . . . . . . . . . . . 9-68
Tip Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-75
Robotic Manipulator Arm (RoMa Standard) . . . . . . . . . . . 9-77
Special Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-77
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-77
RoMa Standard Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-78
Ensuring Operating Readiness . . . . . . . . . . . . . . . . . . . 9-80
Complete RoMa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-81
Mechanical Adjustment after Reinstallation . . . . . . . . 9-83
Grippers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-89
Gripper Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-92
Introduction
This section provides instructions on repair and replacement
of spare parts on the m2000sp.
IMPORTANT: Be sure to verify the instrument you are
! servicing is a G-series and not an E-series model. (For
E-series model service, see the m2000sp E-series Service
Manual.)
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.
WARNING: Class 2 Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing of the bar code laser beam or
reflections of the beam.
WARNING: Electrical Shock Hazard. Possible danger
from electrical shock. Switch off the main power and
disconnect the main power connection of the
instrument before replacing fuses, printed circuit
boards, major sub-assemblies, etc. Replace only fuses
that are externally accessible and labeled. Only use
replacement fuses of the specified type and electrical
rating.
Worktable
NOTE: Two people will be needed to perform this
installation.
CAUTION: The worktable might have sharp edges.
Take care when handling.
Spare Parts
• Worktable
• Positioning pins
• Sealing strip
Removal of Worktable
The worktable must be removed if:
• A new or different type of worktable must be installed.
• Access to the PosID 2 is necessary.
5. Remove the deck pins from the rear two rows of grids 1
and 2, in order for the worktable to slide under the PosID.
6. Align the PosID with the opening created on the
worktable.
Installation of Worktable
1. Carefully install the worktable. If the worktable has deck
pins mounted, remove the rear two rows from grids 1 and
2.
2. Position the PosID on the far right side of the worktable.
3. Insert the oval head screws, and tighten only slightly.
Loosely insert the countersunk screws.
4. Manually align the worktable then further tighten the
front-left screw. This screw acts as a pivot point during the
following alignment.
5. Install the reference tip onto position 3 of the LiHa.
6. Manually position the reference tip at the edge of the
foremost positioning pin in grid position 1 (far left grid).
Check the gap between pin and reference tip.
Verification
Ensure operating readiness by:
• Checking reference positions (Determine LiHa Reference
Position on page 4–60 of Section 4 and Determine RoMa
Reference Positions on page 4–78)
• Configure the PosID 2 on page 4–87
System Electronics
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
OPTIBO Board
The OPTIBO board is located in the left service compartment.
It holds the CU board on its solder side.
OPTIBO
Removal
Perform either a full system shutdown of the SCC or ensure
that the m2000sp application has been closed by opening
Genesis Setup and Software.
1. Verify the instrument is switched off and the power cord
is disconnected.
2. Disconnect all eight cables from the front of the OPTIBO
board.
NOTE: Remember to disconnect the Dilutor cable
(J4) on the solder side of the PCB.
C
H
G D
F
E
Installation
1. Verify the instrument is switched off and the power cord
is disconnected.
2. Ensure the CU board is connected to the OPTIBO board
solder side.
3. Verify that switch SW1 on the OPTIBO board is set to off
(to the right) for both the System and Option CANs.
Jumper SW1
PC Cable
Verification
Ensure operating readiness by performing the following:
• Firmware test
• Initializing of all system devices
• FaWa Fill/Flush Protocol on page 4–122 of Section 4
• PosID Functionality Test on page 4–87
• Random Move test
• CAN-BUS Resistance Test on page 4–33
Fuses
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
NOTE: The fuses are located on the OPTIBO board.
Refer to the following illustration for correct allocation.
CU Board 39-01
Option 1:4
Dilutor
RoMa/R1 R2 39-02
LiHa
CANDI and Uniport or Option 5
Arm L1 L2
CU Board
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
Special Tools
IC release tool
Removal
1. Switch off the instrument.
2. Remove the OPTIBO board as described earlier in OPTIBO
Board on page 9–12; refer to the following illustration.
C
H
G D
F
E
LEDs
J1
IC6
J22 CU Board
Installation
NOTE: Spare CU boards may be delivered with an older
firmware version. If so, firmware upgrade is necessary.
Refer to Firmware Download in Section 11, Appendix
A - Software Reference Material.
1. Determine if jumpers J1 and J22 on the CU board were set
correctly according to configuration of the instrument.
Verification
Ensure operating readiness by initializing all system devices
and performing the following in Setup and Service:
• Firmware test
• Perform Liquid Detection on page 4–127
• Perform FaWa Pump Test on page 4–123
• PosID Functionality Test on page 4–87
• Random Move Test on page 11–141
Removal
1. Verify the instrument is switched off and the power cord
is disconnected.
2. Disconnect the red and black +24VDC power cables from
the DC-DC power supply board.
3. Disconnect the RJ45 cable from the OPTIBO board and
move it to the side.
4. Unscrew the six screws connecting the DC-DC power
supply board to the instrument, and remove the board.
Installation
1. Verify the instrument is switched off and the power cord
is disconnected.
2. If necessary, attach the earth strips to the DC-DC power
supply board using two screws as shown below.
Verification
Ensure operating readiness by performing the following:
• Chiller and Heater Temperature Check on page 5–52 of
Section 5
SMIO Board
The SMIO board is located near the top of the right service
compartment. There are four cables connected:
• J18 System Liquid Capacitance sensor
• J19 Liquid Waste Capacitance sensor
• J21 Solid Waste Ultrasonic sensor
• J2 CAN-BUS cable between the SMIO and SMIO-SAFY
boards
Address Setting: 2
SMIO SMIO-SAFY
J2 CAN-BUS J2 CAN-BUS to OPTIBO Bd
J9 Jumper J3 CAN-BUS to SMIO Bd
J18 Sys Liq sensor J24/J5Left door lock
J19 Liq Wst sensor J23/J6Right door lock
J21 Sld Wst sensor J9 Jumper
SW1 Address switch SW1 Address switch
Figure 9.18: SMIO/SMIO-SAFY
Removal
1. Switch off the instrument.
2. Unplug the four cables.
3. Remove the four screws (M2.5) securing the SMIO board to
the frame.
Installation
1. Set the address of the new board to 2 and ensure the J9
jumper is on the inside two pins.
2. Install the SMIO board to the frame with four screws
(M2.5).
Verification
Ensure operating readiness by performing the following:
• Firmware test
• Verify the green light on the SMIO board is blinking
• Verify the sensors are functioning
SMIO-SAFY Board
The SMIO-SAFY board is located near the bottom of the right
service compartment. There are four cables connected:
J3 CAN-BUS cable between the SMIO and SMIO-SAFY
boards
J2 CAN-BUS cable to the OPTIBO board
J5/J24 Door lock left
J6/J23 Door lock right
Removal
1. Switch off the instrument.
2. Disconnect the four cables.
3. Remove the three screws (M2.5) securing the SMIO-SAFY
board to the frame.
Installation
1. Set the address of the new board to 1 and ensure the
jumper is on the outside two pins.
2. Install the SMIO-SAFY board to the frame with three
screws (M2.5).
Verification
1. Firmware test.
2. Verify the green light on the SMIO-SAFY board is blinking.
3. Verify the door locks are functioning. Verification is
performed through the S&S software. Refer to Check Door
Locks on page 4–115.
Power Module
NOTE: Refer to Section 3, Technical Data for more
information.
WARNING: Electrical Shock Hazard. Switch off the
power and disconnect the power cord of the
instrument before performing any service. The power
module in its entirety is a spare part. For safety reasons,
no replacement of individual power module
components is allowed. Do not attempt to repair this
part. Faulty wiring could potentially harm the user and
damage the instrument.
NOTE: The removal and installation of the power
module takes approximately two hours.
Removal
1. Verify the power switch is off and the instrument is not
connected to the power outlet.
2. Open the top cover.
3. Remove the four screws (M2.5) securing the cover located
to the right of the diluters.
Top cover
33-01(5x M4 x 10) 2
33-03 33
4x M3 x 10
2-02
or
Installation
WARNING: Electrical Shock Hazard. Verify the power
switch is off and the instrument is not connected to the
main (i.e., wall) power outlet.
1. Place the new power module in the instrument.
2. Tape the ends of the power cables together to facilitate
leading the power cables through the cable channel.
3. Secure the power module in place with the five bottom
screws (M3).
4. Secure the power module in place with the five top screws
(M3).
5. Connect the power cables to the OPTIBO board.
6. Install the removed cover.
7. Close the top cover.
8. Connect the power cable to the power outlet and switch
on the instrument.
Verification
Ensure operating readiness by performing the following:
• Perform Liquid Detection on page 4–127
• Random Move Test on page 11–141
X-drive Assembly
Removal
1. Switch off the instrument.
2. Remove the X-bay cover, two screws (M2).
5-01
2.0 mm 5-02
Installation
IMPORTANT: Verify you have the correct G-series part
! (PN 619854) before continuing. E-series X-drives will
not work on G-series instruments.
1. Connect the X-drive assembly to the X-slide with two
screws. Use a feeler gauge to ensure the gap between the
X-drive and X-rod is 2.0 mm.
Verification
Ensure operating readiness by performing the following:
• Perform the Random Move test.
• Check reference points and scale factor.
X-drive Belt
Removal
1. Power off the instrument.
2. Remove the X-drive assembly.
3. Remove the two screws (M3) that secure the cover plate.
Installation
1. Install the X-drive belt onto the pulleys. Ensure the white
guide gear is centered.
2. Attach the cover plate to the X-drive assembly and secure
with two screws.
3. Connect the X-drive assembly to the X-slide.
4. Attach the RoMa or LiHa to the X-drive assembly.
5. Connect any removed cables and secure with clamps.
6. Use a feeler gauge to verify the gap between the X-drive
assembly and X-rod is 2.0 mm.
Verification
Ensure operating readiness by performing the following:
1. Random Move Test on page 11–141
2. Check reference points and scale factor.
Spare Parts
• Y-belt
• DC-Servo board
• DC-Servo Power board
• LiHa board backplane board
• ILID board
• ILID chip
• ILID board flat cable
• ILID cable
• Y DC-Servo motor
• Y-spreading DC-Servo motor
• Solenoid
• DiTi spare set
• Tip adapter
• Lower DiTi Eject assembly
• Drive shaft/pinion gear
Removal
1. Switch off the instrument.
2. Manually move the RoMa all the way to the right side of
the instrument and the LiHa to the middle of the
instrument.
3. Refer to Step 9.34 below to do the following:
• Remove all DiTi adapters (9-03), tubing shelf cover
(9-04), and side covers (9-01).
• Disconnect the LiHa cable (9-05) and X-motor cable
(9-06) from the LiHa backplane board.
• Remove any cable clamps and wraps.
9-06
9-05
9-01
9-04
9-02
9-01 9-03
9-07
9-08
9-09
9-10
9-09
J2
9-11
J1
J3
J1: tip adapter /ILID
J2: NPS/ILID board
J3: NPS/tip
9-08
9-12 9-09
Installation
1. Clean the contact surface of the guide rail, guide rollers,
and support roller with a lint-free tissue and ethyl alcohol.
2. Install in reverse order. Before replacing the support
tubing on the Z-rod:
a. Guide each ILID cable through the Z-rods and
connect to each TIP-ADAPT board.
b. Guide the pipetting tubing through the Z-rods and
connect each one to their respective tips.
Verification
Ensure operating readiness by performing the following:
• Flush tips sequentially to verify tubing routing through
Genesis Setup and Service <System>, <Devices>,
<Dilutor>.
• Tip Adapter Test
• Check reference position
• Random Move test
• Liquid Detection test
• Lower DiTi Eject test
• Precision test
• CAN-BUS Resistance Test on page 4–33
• m2000sp calibrations (LiHa)
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26
– LiHa DiTi Location Test on page 5–12
– Sample Extraction Test on page 5–37
DC-Servo
Removal
1. Switch off the instrument.
2. Remove instrument rear panel.
3. Unplug X-drive Power Cable and Lower DiTi Eject power
cable from the backplane board. Remove the boards
housing cover (four screws - M2). Refer to Figure 9.26:
X-motor Cable Connection on page 9–33 for details.
4. Remove the DC-Servo boards and DC-Servo Power board.
Keep the DC-Servo boards in order because each board has
a unique address.
5. Remove the boards housing from the back of the LiHa
(four screws - M2.5).
9-14
9-13
9-17
9-16
9-15
6. Disconnect the ILID board flat cable and all Y-motor and
Z-motor cables from the LiHa board backplane board and
lower DiTi Eject Assembly cable (J3).
Installation
1. Ensure the bus termination switch is set to on.
2. Attach the LiHa backplane board to the bracket with six
screws (M2.5).
3. Connect the ILID board flat cable and all Y-motor and
Z-motor cables to the backplane board, and the Lower DiTi
Eject Assembly cable to J3.
NOTE: The cable connectors and board
connections are labeled. Be sure to install the
cables in the correct order. Refer to Table 9.2:
Board Positions on page 9–48.
4. Install the boards housing to the back of the LiHa with
four screws (M2.5).
5. Install the DC-Servo boards and DC-Servo Power board in
the correct order.
6. Attach the boards housing cover with four screws (M2).
Verification
Ensure operating readiness by performing the following:
• Check reference position
• Random Move test
• Liquid Detection test
• Lower DiTi Eject test
• Precision test
• CAN-BUS Resistance Test on page 4–33
• m2000sp calibrations (LiHa)
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26
Removal
1. Switch off the instrument.
2. Remove the LiHa from the instrument, or if access to the
rear of the instrument is available, remove the instrument
back cover. Refer to Complete LiHa Assembly on page
9–40.
3. Refer to Figure 9.38: LiHa DC-Servo Boards on page 9–44:
• Remove the boards housing cover (9-15), (four
screws).
• Remove the appropriate DC-Servo board (9-14) or the
DC-Servo Power board (9-13).
Large, Brown
Resistor
Green Resistor
Installation
Install in reverse order.
NOTE: Before installing a new DC-Servo board, use a
2 mm flathead screwdriver to dial the correct address
on the address switch of the replacement DC-Servo
board.
Table 9.2: Board Positions
Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• Check Each of the Tip Adapters on page 4–58
• Determine LiHa Reference Position on page 4–60
• Perform the Lower DiTi Eject Test on page 4–64
• Perform Liquid Detection on page 4–127
• LiHa Calibrations on page 4–134
ILID Board
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
1. Switch off the instrument.
2. Remove the tubing shelf cover, four M2 screws, and left
side cover. Refer to Figure 9.34: LiHa Assembly on page
9–40.
3. Disconnect the ILID board flat cable (J9) and all eight ILID
cables from the ILID board. Refer to Figure 9.35: LiHa
Removal of ILID Cables and Tubing from Z-rod on page
9–41.
C
A B
Installation
Install in reverse order.
Be sure to set the ILID board switches (2 switches) SW1 and
SW2 to eight for the number of tips.
Verification
Ensure operating readiness by the following: Perform Liquid
Detection on page 4–127.
Removal
1. Switch off the instrument.
2. Remove the left side cover and the tubing shelf cover (9-01
and 9-04 on Figure 9.34: LiHa Assembly on page 9–40)
(eight screws - M2).
3. Disconnect the ILID board flat cable from the ILID board
(9-12) and LiHa backplane board (9-17) Refer to Figure
9.35: LiHa Removal of ILID Cables and Tubing from Z-rod
on page 9–41 and Figure 9.38: LiHa DC-Servo Boards on
page 9–44.
Installation
Install in reverse order.
Verification
Ensure operating readiness by the following: Perform Liquid
Detection on page 4–127.
ILID Cable
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
1. Switch off the instrument.
2. Remove the left side cover (9-01 on Figure 9.34: LiHa
Assembly on page 9–40) (four screws - M2).
3. Refer to Figure 9.35: LiHa Removal of ILID Cables and
Tubing from Z-rod on page 9–41 to do the following:
• Disconnect the ILID cable (9-09) from the TIP-ADAPT
board (9-11) and ILID board (9-12).
• Remove the support tubing (9-07) from the Z-rod
(9-10).
• Remove the ILID cable (9-09) and pipetting tubing
(9-08) from the Z-rod (9-10) and support tubing
(9-07).
Installation
1. Install in reverse order:
a. Guide the ILID cables through the Z-rods and support
tubing.
b. Guide the aspiration tubes through the Z-rods and
support tubing.
2. Attach the ILID cable to the ILID board (Tip Connector,
NOT Piezo connector) and TIP-ADAPT board (J1).
3. Attach the left side cover to the LiHa with four screws
(M2).
Verification
Ensure operating readiness by the following: Perform Liquid
Detection on page 4–127.
Y-belt
Removal
1. Switch the instrument off.
2. Remove the LiHa from the instrument (refer to Complete
LiHa Assembly on page 9–40).
3. Remove the left side cover and the tubing shelf cover (9-01
and 9-04 on Figure 9.34: LiHa Assembly on page 9–40)
(eight screws - M2).
4. Refer to Step 9.43 below.
• Remove the Y DC-Servo motor (four screws) or
Y-spreading DC-Servo motor (four screws) to slacken
the Y-belt.
9-18 9-19
9-20
5. Remove the Y-belt lock from the Y-slide (two screws - M2)
and take the Y-belt out of the LiHa.
9-21
9-22
9-23
Installation
1. Install in reverse order. Belt length is 1290 mm/50.8 in.
2. Insert the Y-belt assembly into the arm belt lock and close.
3. Insert the Y-belt assembly into the LiHa. Do not attach the
Y-belt to the motor pulley at this time.
4. Move the Y-belt and lock into position on the Y-slide.
5. Pull the Y-belt over the Y-motor pulley.
6. Manually tighten the Y-belt by moving the Y-DC-Servo
motor assembly.
7. Tighten the four screws securing the motor assembly.
8. Install the cover.
9. Install the LiHa.
Verification
Ensure operating readiness by performing the following:
• Determine LiHa Range on page 4–60
• Determine LiHa Reference Position on page 4–60
• Random Move Test on page 11–141
Removal
1. Remove the LiHa from the instrument. Refer to Complete
LiHa Assembly on page 9–40 for details.
9-19
9-20
Installation
1. Install in reverse order.
2. Install the pinion gear onto the motor.
Verification
Ensure operating readiness by performing the following:
• LiHa Range calibration reference position calibration
• m2000sp calibrations (LiHa)
• Random Move test
• CAN-BUS Resistance test
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
Removal
1. Remove the LiHa from the instrument. Refer to Complete
LiHa Assembly on page 9–40 for details.
2. Remove the screw of the front bushing of the Z-axis drive
shaft/pinion gear to be replaced. See Figure 9.47 below.
Screw
Bushing
A Set screw
B Drive shaft
C Retainer screws
A Pin
B Slot
C Z-guide
A Retainer screw
B Retainer
C Z-rod
Set screw
ILID cable
Z-rod guide
Pinion gear
Z-axis
10. Repeat the above process for the remaining seven drive
shaft/pinion gears.
Installation
Install the drive shaft and pinion gear Z-axis in reverse order of
removal. However, be mindful of the following:
• Do not lubricate the parts.
• Do not apply force when pushing in the Z-rod; turn the
pinion gear Z-axis in such a way that it engages properly
in the Z-rod.
• If the spreading link pin was removed, make sure it is
properly reinstalled in the slot of the Z-rod.
Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• Determine LiHa Range on page 4–60
• Determine LiHa Reference Position on page 4–60
• Calibrate the Lower DiTi Eject on page 4–63
• Perform the Lower DiTi Eject Test on page 4–64
• Y-Spread Procedure on page 4–67
DiTi Mechanism
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
(Type B)
Please Note
If your instrument was installed prior to
April 2007 and DOES NOT bear the label
shown here, refer to White Separator Ring
and Black O-ring Assembly on page 9–67
before performing the DiTi Adapter
Replacement.
Removal
1. Switch off the instrument and open the front safety panel.
2. Manually move up all Z-racks until they touch lightly,
then move the entire assembly toward the front of the
instrument. Spread the Z-racks completely apart.
3. Loosen the DiTi cone with the supplied wrench. Support
the tip adapter while loosening the cone.
NOTE: If the adapter cylinder within the black tip
ejector tube turns instead of the DiTi cone
(preventing DiTi cone removal), refer to Removal
of DiTi Cone with Loose Adapter Cylinder below.
4. Remove the DiTi cone and ejector tube.
5. Remove the O-ring.
6. Pull out the tubing extension and disconnect the tubing.
7. Unscrew and remove the adapter cylinder.
1. Place the pliers on the tip ejector tube over the knurled
portion of the adapter cylinder, which will be found just
below the rectangular tip adapter.
2. Gently squeeze the pliers.
NOTE: If the pliers are not placed over the knurled
surface, they will not prevent the cylinder from
spinning regardless of the pressure applied.
3. Place the DiTi cone wrench on the DiTi cone.
Installation
1. Pull the pipetting tubing approximately 10 cm out of the
tip adapter.
2. Screw the adapter cylinder (knurled part up) onto the tip
adapter. Finger tighten only.
Separator ring
(white)
O-ring (black)
Spare Parts
The following items are available:
• Lower DiTi eject option
• Solenoid
1 Z-rod #3
2 5 Solenoid
3. Unscrew the fixing screw (4) from the housing of the third
Z-rod (1).
4. Unscrew fixing screws (6) and (2) then remove the
complete lower DiTi eject option assembly.
Verification
Ensure operating readiness by performing the following:
• Calibrate Lower DiTi eject.
• Perform the Lower DiTi Eject test.
• Perform m2000sp Calibration (LiHa)
Removal of Solenoid
1. Remove the front and right LiHa covers.
2. Disconnect the solenoid cable (7 on Figure 9.56: Removal
of Lower DiTi Eject Option on page 9–68) from the socket
J3 on the backplane and free the cable from cable holders
and velcro tape.
14
13 10
12 11
Installation of Solenoid
1. Install in reverse order.
NOTE: Verify the solenoid holder screws are
tightened.
2. Connect the solenoid cable to the backplane.
3. Verify that the thin part of the lever is approximately in
the center of the fork and is at a 90-degree angle to the
drive shaft. When pushing the drive shaft in the
Y-direction (to the front and rear), notice that the lever
does not touch the fork on the left or right.
4. Perform the Rocker Adjustment Procedure on page 9–71.
Verification
Ensure operating readiness by performing the following:
Solenoid
Fixing nut
15
18
17
16
16 Rocker assembly
17 Rocker shaft
Solenoid
Fixing nut
Verification
• Verify/adjust the LiHa reference positions
• Perform m2000sp Calibrations (LiHa)
• Calibrate Lower DiTi Eject
• Perform the Lower DiTi Eject test
• The rocker must not touch the Z-rods on the LiHa. If it
does, re-perform the rocker adjustment.
Tip Adapter
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
NOTE: G-series instruments use Type B tip adapters
only. G-series and E-series tip adapters are not
interchangeable. (E-series models use Type D tip
adapters.)
Removal
1. Remove the DiTi adapter.
2. Remove the cover from the TIP-ADAPT board and
disconnect the ILID cable from the TIP-ADAPT board.
3. Remove the set screw (1.5 M) holding the tip adapter to
the Z-rod.
4. Hold on to the Z-rod with one hand and carefully pull out
the tip adapter from the bottom of the Z-rod.
Installation
1. Slide the Type B tip adapter up into the bottom of the
Z-rod until the shoulder of the tip adapter shaft (below the
alignment hole) is flush with the base of the Z-rod.
2. Align the screw hole in the tip adapter shaft with the set
screw hole in the Z-rod and secure the tip adapter to the
Z-rod with the set screw.
Type B tip
adapter
Verification
• Perform the M&D Sample Extraction Test on page 5–37.
See Section 5, Maintenance and Diagnostic Procedures,
for more information.
Special Tools
• Mounting bracket
• RoMa reference tip
• Black test plate
Overview
The m2000sp uses the RoMa Standard assembly. The RoMa
Standard and Long assemblies use common parts and either
can be shown for illustration purposes.
Eccentric Gripper
The spare part designated “Eccentric Gripper” (14-01) is a set
that includes the following components:
• One long gripper finger (14-21)
• One short gripper finger (14-22)
14-22
14-17
14-18
14-16
Purpose
Most procedures described in this section require the entire
RoMa to be removed before a damaged part can be replaced.
Therefore, adjustments and tests described below must be
performed after reinstallation.
Adjustments, Tests
1. Adjust the RoMa mechanically as described in Mechanical
Adjustment after Reinstallation on page 9–83.
2. Perform the CAN-BUS Resistance test if the RoMa Freedom
backplane, located at the rear, was replaced.
If the RoMa was removed or a new RoMa was installed,
perform Genesis Setup and Service RoMa Calibrations,
and m2000sp calibrations.
3. Perform the Rotator Test in Setup and Service.
4. Perform the following M&D checks from Section 5:
• RoMa DiTi Rack Test on page 5–27
• RoMa DiTi Shelf Test on page 5–29
• RoMa 1 mL Carrier Test on page 5–30
After Finishing
1. Reinstall all covers that were removed.
2. Close service doors and X-bay covers.
Complete RoMa
Removal
Perform the following steps to remove the RoMa (14).
14-34
14-27
14-28
14
14-33 14-31
14-32
14-29
14-30
14-01
14 RoMa 14-31X-support
14-27Mounting bracket 14-32X-slide
14-28Guide rollers 14-33X-shaft
14-29Support roller 14-34X-flex cable
14-30Guide rail at front
7. Carefully lift the RoMa out of the guide rail (14-30) and off
the X-slide (14-32).
Installation
1. Clean the contact surface of the guide rail, and the guide
rollers and support roller with a lint-free tissue and a small
amount of ethyl alcohol.
2. Insert the mounting bracket to press the guide rollers
(14-28) apart.
3. Install the RoMa onto the guide rail and onto the X-slide.
4. Insert the four screws, but do not completely tighten.
5. Remove the mounting bracket.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Purpose
After the RoMa is installed, it must be aligned with the guide
pins on the worktable. The purpose of this alignment is to
ensure the RoMa runs parallel to the guide pins when it moves
in the Y-axis. (rear ↔ front).
14-37
14-38
14-39
14-40
14-41
Alignment in Y-axis
1. Pull the gripper module head (14-37) downward.
14-37
14-41
14-42
14-41
14-42
02
Figure 9.74: Checking the Distance Guide Pin/Reference Tip
14-45 14-48
14-47
14-46
14-43
14-44
2. If the RoMa drifts to the right, turn the right set screw
(14-43) to correct.
3. If the RoMa drifts to the left, turn the left set screw (14-46)
to correct.
4. Repeat steps 1 through 3 until the RoMa Standard moves
parallel to the guide pins.
0.2 mm
0.5 mm
0.2 mm
0 mm
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Grippers
Removal
The RoMa gripper set includes:
• 2 gripper fingers of different lengths
– Short gripper finger: 131 mm
– Long gripper finger: 161 mm (has oblong hole
4.2 mm for height adjustment)
• 2 screws
– 1 shoulder-head screw for the short finger
– 1 flat-head screw for the long finger
• 2 pieces of 3M foam tape (for the reference plate)
Flat-head screw
14-38 14-21
Shoulder screw
14-22
Removal
Loosen the screws and unscrew the long (14-21) and short
(14-22) gripper fingers.
NOTE: Ensure the shoulder screw stays with the short
finger and the flat-head stays with the long finger.
Installation
1. Install the short gripper finger (14-22) and tighten the
shoulder screw. Ensure it is installed on the correct side as
illustrated in Figure 9.77 above.
2. Position the long gripper finger (14-21) and fasten it
slightly using the flat-head screw.
Verification
• Perform all required RoMa S&S calibrations per this
manual and the latest version of ISA 605-002, including:
– Determine RoMa Reference Positions on page 4–78 of
Section 4
– Determine Range on page 4–81
– Adjust the Rotator on page 4–82
– Manual RoMa Rotator Adjustments on page 4–83
• Perform all RoMa m2000sp calibrations per ISA 605-002;
refer to RoMa Calibrations on page 4–157
• Perform the following M&Ds:
– RoMa DiTi Rack Test on page 5–27 of Section 5
– RoMa DiTi Shelf Test on page 5–29
– RoMa 1 mL Carrier Test on page 5–30
Gripper Board
Overview
14-56
14-57
14-58
14-06
14-54
14-55
14-67 14-04
14-59 14-62
14-60 14-66
14-63
14-0514-69
14-64
14-65
14-68
14-03 14-53
14-22
14-52
14-37 14-21
Unplug Cables
1. Unscrew the three screws (1.5M) that secure the rotator
cover (14-52).
NOTE: Refer to Figure 9.79: Overview of Rotator
and Gripper Modules on page 9–92 for details.
Extract Wires
Use the crimp release tool to extract individual wires from the
connector (14-60). Be careful to not damage the contact
bushes (14-61). Refer to Figure 9.79: Overview of Rotator and
Gripper Modules on page 9–92 for details.
14-60
14-61
" !
$ #
& %
Figure 9.84: Gripper Motor Cable Plug
14-60
14-61
" !
$ #
& %
Figure 9.87: Wire Colors
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Gripper Module
Removal
1. Remove the grippers.
2. Remove the gripper board.
3. With one hand, hold the gripper module. Remove the two
gripper module screws (2.5M) using the other hand.
5. With one hand, hold the gripper module. Lead the gripper
cable through the Z-module using the other hand.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Rotator Motor
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
Refer to Figure 9.79: Overview of Rotator and Gripper Modules
on page 9–92 for details.
1. Remove RoMa and gripper board as previously described
(see pages 9–81 and 9–92).
2. Loosen the gripper module head screws (14-68) to access
the fixing screws (14-69) of the rotator motor (14-05).
Refer to Gripper Board on page 9–92.
3. Unscrew and remove the rotator motor (14-05).
Screws
Installation
1. Reinstall the rotator motor (14-05).
2. Tighten the gripper module head screws (14-68).
3. Reinstall the gripper board and RoMa.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Y-flex Cable
Removal
14-16
14-15
14-10
14-10 RoMa 2 backplane
14-15 Y-flex cable
14-16 RoMa Freedom backplane
A
B
C
D
C
D
Installation
1. Install in reverse order.
NOTE: Pleat the new flex cable in the same
manner as the old one.
Figure 9.101:
General
The gripper/rotator flex cables are two parallel cables—for the
rotator motor and gripper motor—which are connected to the
RoMa backplane and gripper board.
Removal
14-56
14-57
14-08
14-09
A
C
B
Installation
Install in reverse order.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
14-09 14-70
14-08
14-71
14-70
Removing Boards
1. Remove the left cover (nine screws) and disconnect the
Y-flex cable.
A
A
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.
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.
* + ,-
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
RoMa 2 Backplane
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
1. Remove the left cover (nine screws).
2. Disconnect six cables from the RoMa 2 backplane (14-10).
14-09
14-10
14-73
14-08
14-70
14-72
14-73 14-74
RoMa 2
backplane
Z/G-DC-Servo
board
Installation
Perform steps above in reverse order:
• Install the RoMa 2 backplane.
• Install the Z/G- and Y/R-DC-Servo boards.
• Connect six cables to the Roma 2 backplane.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Y-belt
Removal
1. Remove the RoMa. Refer to Complete RoMa on page 9–81.
14-77
14-75 14-80
14-11
14-79
14-78
4. Loosen the belt fixing screws (M2) (14-79) and remove the
front end of the Y-belt.
5. Loosen the screws (M2) (14-82) at the rear until the rear
end of the Y-belt (14-11) can be removed.
14-11
Installation
14-81
14-79
14-78 14-11
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Y-motor
Removal
14-77 14-80
14-11 14-83 14-75
14-10
14-87
14-12
14-90
14-88 14-79
14-78
14-89 14-89
2. Cut the cable tie (14-88) and disconnect the motor cables
(14-89) and (14-90) from the RoMa 2 backplane (14-10).
3. Loosen screws (14-77) and (14-80) until the screws (14-79)
become accessible. This releases the belt tension.
Installation
1. Insert the Y-motor. Ensure the encoder head points in the
correct direction.
2. Secure the motor to the RoMa with three fixing screws
(M2).
3. Place the Y-belt pulley over the motor axle. Ensure the flat
sector of the Y-motor axle is perpendicular to the pulley
screw.
4. Install the support plate.
5. Secure and tension the Y-belt.
6. Connect the motor cables to the RoMa 2 backplane.
7. Install the left cover with seven screws.
8. Install the RoMa into the instrument.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Z-motor
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
14-09
14-08
14-93 14-92
14-91
14-96 14-10
14-94
14-95
14-13
Figure 9.131:Z/G-DC-Servo
Installation
1. Place the replacement Z-motor on the support. Ensure the
motor encoder head points in the correct direction.
2. Insert and tighten the three motor fixing screws.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Z-brake
The old brake assembly featuring the shoe with the curved face
is being phased out. Upon failure, it should be replaced with
BRAKE SOLENOID Z-AXIS COMPLETE ROMA-3 (part number
30027047).
Z-brake Removal
14-100
14-56
14-14
14-98
14-97
14-10
14-14
14-99
A
B
C
Z Brake
Z-brake Installation
NOTE: The brake shoe is screwed to the axle and fixed
with the set screw.
NOTE: For replacement of the brake shoe only, refer to
Brake Shoe Replacement on page 9–130 below.
1. Press the brake shoe down to the stop as shown below.
A Brake shoe
B Set screw
C Axle
d = Distance
Verification
Refer to Ensuring Operating Readiness on page 9–141.
A Brake shoe
B Set screw
C Axle
d = Distance
4. Install the new spring and shoe, and secure with the set
screw.
5. Press the shoe down to the stop and measure the distance
between the brake shoe and the body. The distance must
be 7.25 + 0.2 mm (0.285 + 0.008 in).
6. Adjust as needed by loosening the set screw and turning
the brake shoe to achieve the correct distance.
7. Re-install the assembly as described in Z-brake Installation
on page 9–129.
Overview
F A
D
E
B
Removal
1. Remove the RoMa or, if access allows, remove the back
cover.
14-104
14-17
14-18
14-103
14-16
14-105
Installation
1. Set the address to 4 and verify jumper J2 is terminated.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Device CU
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
14-104
14-17
14-18
14-16
14-106
14-103
14-105
Installation
1. The address switch (14-106) for the Device CU board
(14-17) is located on the RoMa Freedom backplane. Set the
address switch on the RoMa to position 4.
Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
NOTE: If the Device CU board is exchanged without
restoring the EEPROM, the following setups must be
performed:
• Set X-drive properties
• Set machine size
• RoMa setup
Removal
1. Remove the RoMa, or if access is allowed remove the back
cover.
2. Remove the lower board retaining clamp (white-headed
push pin) (14-71).
14-104
14-17
14-18
14-16
14-106
14-103
14-105
Installation
1. The address switch (14-106) for the X-DC-Servo Power
board (14-18) is located on the board itself. Set the address
switch to position 0 (zero).
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.
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Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
Purpose
Most procedures described in this section require the entire
RoMa to be removed before a damaged part can be replaced.
Therefore, adjustments and tests described below must be
performed after reinstallation.
Adjustments, Tests
1. Adjust the RoMa mechanically as described in Mechanical
Adjustment after Reinstallation on page 9–83.
2. Perform the CAN-BUS Resistance Test on page 4–33 if the
RoMa Freedom backplane, located at the rear, was
replaced.
If the RoMa was removed or a new RoMa was installed,
perform Genesis Setup and Service RoMa Calibrations,
and m2000sp RoMa Calibrations on page 4–157.
3. Adjust the Rotator on page 4–82.
4. Perform the following M&D checks:
• RoMa DiTi Rack Test on page 5–27
• RoMa 1 mL Carrier Test on page 5–30
See Section 5, Maintenance and Diagnostic Procedures,
for more information.
After Finishing
1. Reinstall all covers that were removed.
2. Close service doors and X-bay covers.
3. Return objects that were removed to the worktable.
4. Start the m2000sp in operating mode.
PosID 2
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
WARNING: Class II Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing of the bar code laser beam or
reflections of the beam.
Spare Parts
• PosID 2 assembly
• X-belt
• X-flex cable
• X-DC-Servo motor
• PosID 2 CU board
• X-DC-Servo board
• Y/B board
• Y/B-DC-Servo board
• DSP decoding board
• B-DC-Servo motor
• Scanner head
• Scanner cable
• Y-belt
• Gripper assembly
• Y-DC-Servo motor
• No Tube sensor
Removal
1. Switch off the instrument.
2. Remove the left and right door locks.
3. Remove the worktable; see Worktable on page 9–8.
4. Disconnect the PosID 2 connection cable (13-03) and
X-motor cable (13-02) from the PosID 2 CU board.
13-01
13-02
13-04
13-03
13
13-10
13-09
13-08
13-07
13-06
13-02
13-03
13-05
Installation
1. Install in reverse order.
13-08
13
Verification
Ensure operating readiness by performing the following:
• Worktable Alignment
X-belt
Removal
1. Switch off the instrument.
2. Remove the worktable; see Worktable on page 9–8.
3. Loosen the belt tensioning screws (13-13) (two screws).
Installation
1. For a new belt, cut the X-belt to the required length: 2430
mm.
2. Run the X-belt over the idler pulley (13-12) and drive
pulley of the X-motor; insert belt ends into the fixture and
tighten the fixing screw (13-14).
13-12
13-13
2x M4x8
13-05
13-14 M4x20
Verification
Ensure operating readiness by performing the following:
• Align the worktable as described in Installation of
Worktable on page 9–9
• Configure the PosID 2 on page 4–87
• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
– CAN-BUS Resistance Test on page 4–33
X-motor
Removal
1. Switch off the instrument.
2. Remove the worktable; see Worktable on page 9–8.
3. Disconnect the X-motor cable (13-02) from the PosID
board (13-07). Refer to Figure 9.156: PosID 2 Assembly on
page 9–145 for details.
Installation
Install in reverse order.
• Adjust the distance between the drive pulley and bracket
to approximately 5.5 mm.
Verification
Ensure operating readiness by performing the following:
• Worktable alignment (see Installation of Worktable on
page 9–9)
• Configure the PosID 2 on page 4–87
• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
• CAN-BUS Resistance Test on page 4–33
X-flex Cable
Removal
1. Switch off the instrument.
2. Remove the worktable; see Worktable on page 9–8.
3. Remove casing as illustrated in Figure 9.165: Y/B Board
Assembly on page 9–155.
4. Disconnect the X-flex cable from PosID board. Refer to
Figure 9.156: PosID 2 Assembly on page 9–145.
4x M3x6
2x M3x8
13-20
5x M2x6
Installation
1. Fold the X-flex cable on the end where it will be connected
to the PosID.
185 mm (7¼”)
185 mm (7¼”)
Figure 9.163:X-flex Cable
Verification
Ensure operating readiness by performing the following:
• Worktable alignment (see Installation of Worktable on
page 9–9)
• Configure the PosID 2 on page 4–87
• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
• CAN-BUS Resistance Test on page 4–33
Electronic Boards
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
1. Switch off the instrument.
2. Disconnect all cables from the PosID 2 CU board.
3. Unscrew and remove X-DC-Servo board (two screws).
J8
J6
Installation
1. Verify jumper J6 (for L-CAN Bus) is set (jumper closed).
2. Verify jumper J8 is set according to the communication
diagram (jumper open).
3. Verify the X-DC-Servo board address switch is set to #0.
4. Install in reverse order of removal.
Verification
Ensure operating readiness:
• CAN-BUS Resistance Test on page 4–33
• Configure the PosID 2 on page 4–87
Removal
13-17
13-16 13-18
13-15
3x M2x6
Figure 9.166:PosID
13-20
5x M2x6
Installation
1. On the Y/B-DC-Servo board, verify the address switch is
set to #2.
2. Attach the DSP decoding board to the Y/B board with
three screws.
3. Connect two cables from the DSP decoding board to the
Y/B board.
4. Install the Y/B-DC-Servo board to the Y/B board. Secure
with the white-headed push pin.
Scanner Cable
X-flex Cable
Y-motor Cable
Connections to DSP
Board
B-motor Cable
No Tube Sensor Cable
Verification
Ensure operating readiness:
• Configure the PosID 2 on page 4–87
Removal
NOTE: The referenced position numbers are illustrated
in Figure 9.164 on page 9-144.
1. Remove the three screws that secure the casing (13-15).
Figure 9.174:PosID
Installation
1. Attach the DSP decoding board to the Y/B board with
three screws.
2. Connect two cables (J7 and J9) from the DSP decoding
board to the Y/B board.
3. Attach the casing with three screws.
Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87
B-motor
Removal
1. Remove the three screws that secure the casing (13-15) as
illustrated in Figure 9.165: Y/B Board Assembly on page
9–155.
2. On the exterior of the PosID 2, unscrew the five Y/B board
fixing screws (13-20) to free the B-motor cable (13-24).
Disconnect this cable from Y/B board (J4).
13-19
2x M4x8
13-20
13-17 5x M3x6
13-24
13-23
13-22
M5x6
4x M2.5x8 13-21
Installation
1. Install in reverse order.
NOTE: Observe correct positioning of the encoder
head as illustrated in Figure 9.177: B-motor on
page 9–162.
2. Use four screws to attach the B-motor to the bracket.
3. Tighten the set screw on the motor gearing assembly.
4. Secure the B-motor assembly to the PosID assembly with
two exterior screws.
5. Connect the B-motor cable to the Y/B board at J4.
6. Secure the Y/B board to the PosID assembly with five
screws.
7. Attach the casing with three screws.
Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87
Removal
1. Remove the three screws securing the casing (13-15) as
illustrated in Figure 9.165: Y/B Board Assembly on page
9–155.
13-25
13-28
13-29
13-26 13-27 Potential
sticking point
Installation
1. Install in reverse order.
NOTE: Swivel the scanner head up and down and
arrange the cable (13-29) so it does not touch and
stick at the point indicated with an arrow in Figure
9.179: Scanner Head Assembly on page 9–164.
2. Attach the scanner head with two screws.
3. Connect the scanner cable to the Y/B board.
4. Connect the cable holders and cable connector.
5. Attach the scanner head housing with two screws.
6. Attach the casing with three screws.
Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87
Removal
1. Remove three screws (M2) securing the casing (13-15)
Figure 9.165: Y/B Board Assembly on page 9–155.
2. Loosen the two belt tensioning screws (M2.5) (13-36 in
Step 9.183 below).
13-30
13-31
13-32
4x M3x6
13-33
13-34
13-35
2x M2x6 13-36
Installation
1. Lead the Y-belt around the driver pins (bottom side of
gripper assembly) as shown below.
Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87
Y-motor
Removal
1. Remove casing (13-15) as illustrated in Figure 9.165: Y/B
Board Assembly on page 9–155.
2. Loosen the two belt tensioning screws (M2.5) (13-36).
Refer to Figure 9.183: Y-drive Assembly on page 9–167 for
details.
2x M3x8
Figure 9.189:Y-motor
Installation
Install in reverse order.
• Note the correct orientation of the encoder head. Refer to
Figure 9.183: Y-drive Assembly on page 9–167 for details.
• When installing the drive pulley, ensure the set screw is
perpendicular to the flat part of the axle.
• Tension the Y-belt as described earlier in Y-belt, Gripper
Assembly on page 9–166, for details.
Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87
No Tube Sensor
Removal
NOTE: The referenced parts are illustrated in Figure
9.183: Y-drive Assembly on page 9–167.
1. Switch off the instrument.
2. Remove the casing (13-15) as illustrated in Figure 9.165:
Y/B Board Assembly on page 9–155.
3. Unscrew the cable clamps (M2.5) then disconnect the No
Tube sensor cable (13-32) from the Y/B board.
4. Remove the two screws (M1.5) used to secure the No Tube
sensor (13-35).
Installation
Install in reverse order.
1. Place the No Tube sensor into the PosID assembly and
secure with two screws.
2. Route the No Tube sensor cable to the Y/B board and
connect the cable to the board.
3. Secure the cable in place with the cable clamps.
4. Install the casing.
Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87
Liquid System
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. The exposure to hazardous chemicals is
minimized by following instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.
Overview
The term “liquid system” refers to all instrument modules and
parts which contain or directly influence liquid. The main
components include:
• LiHa Freedom; refer to Liquid Handling Arm (LiHa) on
page 9–39
• Tips; refer to the m2000sp Operations Manual
• Tubing system; refer to Tubing System below.
• MPO/FWO; refer to Monitored Pump Option/Fast Wash
Option on page 9–176
• Dilutor/Dilback; refer to Dilutors on page 9–182
Tubing System
This section describes the standard tubing system. Refer to
Monitored Pump Option/Fast Wash Option later in this
section for MPO/FWO tubing system details.
Spare Parts
Tubing and fittings for standard tubing
Standard Tubing
8 Tips
30-03
30-02
8
30-01
4
30-06 32 9
30-07
30-04
35
30-05
Replacement
1. Empty the liquid system.
2. Switch off the instrument.
3. Replace relevant tubing.
NOTE: When replacing the pipetting tubing,
remember that diluter #1 (on the far left) is
connected to tip #1 (closest to the rear of the
instrument), diluter #2 to tip #2, etc.
Verification
Ensure operating readiness by performing the following:
• Tightness check
• Perform FaWa Pump Test on page 4–123
• The following M&D checks from Section 5:
General
MPO/FWO Complete
Removal
1. Empty the liquid system.
2. Switch off the instrument.
3. Remove the two outer screws of the left worktable cover
(M2.5 x 8).
2 screws M2.5 x 8
Figure 9.192:Fast Wash Pump Assembly Removal
Installation
Install in reverse order.
• Electronic connections: OPTIBO, Option CAN-MPO
board, J7
• Connect the tubing as shown in Step 9.193 below
35-1
35-8
35-7
35-6
35-5
MPO/FWO Standard
Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
Removal
1. Remove the Fast Wash pump assembly from the
instrument.
2. Remove the tubing from the FaWa pump.
3. Remove the FaWa pump from the Fast Wash pump
assembly (five screws).
Installation
1. Install in reverse order. Connect the tubing as shown in
Figure 9.193: MPO/FWO Tubing Connections on page
9–178
2. Use the Dilutor panel of the LiHa to fill the Fast Wash
pump. A dry pump valve may not work properly.
Verification
Ensure operating readiness by performing the following:
Removal
1. Remove the Fast Wash pump assembly from the
instrument.
2. Remove the tubing from the pressure relief valve.
3. Remove the pressure relief valve from the Fast Wash pump
assembly (1 screw).
NOTE: Be careful not to confuse the attachment
screw of the pressure relief valve with the
regulating screw. Do not alter the setting of the
regulating screw.
Installation
Install in reverse order. Ensure the arrows on the valve match
the direction of fluid flow as shown above in Figure 9.195.
Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
MPO/FWO Tubing
Removal
1. Remove the Fast Wash pump assembly from the
instrument.
2. Remove the tubing.
Installation
Install in reverse order. Refer to Figure 9.193: MPO/FWO
Tubing Connections on page 9–178 for correct tubing
connections.
Verification
Ensure operating readiness:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
MPO Board
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Removal
1. Remove the Fast Wash pump assembly from the
instrument. The MPO board is located at the backside of
the assembly.
2. Disconnect the FaWa pump cable and connection cable.
3. Remove the MPO board (5 screws).
Installation
Install in reverse order.
• Connection cable: J7
• FaWa pump cable
Go into the <Liquid System>, <FaWa>, <Setup> menu, and
set the number of pumps to 1 and number of tips for pump 1
to 8.
Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
Dilutors
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Spare Parts
• Dilutor type XP 3000 plus
• 3-way valve, 120°, M6 fittings
• Syringe
• VCC-Dilback
Dilutor
Removal
1. Empty the liquid system using the Dilutor Panel of the
LiHa.
2. Switch off the instrument.
3. Move the LiHa and RoMa arms to one side.
4. Open top cover (2 screws).
5. Remove diluter case cover (2 screws).
2-01
2-02
Installation
1. Verify the CAN-BUS address is set correctly.
Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
• Perform Liquid Detection on page 4–127
• Perform the following M&D checks:
– Dispense Test 50 µL, Visual on page 5–21
– Dispense Test 800 µL, Visual on page 5–24
See Section 5, Maintenance and Diagnostic Procedures
for more information.
Removal
1. Unscrew the knurled plunger lock screw that secures the
syringe to the plunger drive block.
Valve connection
3-way valve
Syringe screw
M3 x 25
4. Remove the two phillips head screws (M3 x 25) that secure
the valve to the diluter and remove the valve.
Installation
1. Secure the 3-way valve to the diluter using two phillips
head screws.
2. Connect the input tubing (from the 1:4 distributor) and
the output tubing (to the DiTi) to the 3-way valve.
3. Insert the syringe and tighten the syringe screw.
4. Tighten the knurled plunger lock screw to secure the
syringe to the plunger drive block.
Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
• The following M&D checks from Section 5:
– Dispense Test 50 µL, Visual on page 5–21
– Dispense Test 800 µL, Visual on page 5–24
VCC-Dilback
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
Removal
NOTE: The VCC-Dilback is positioned in the diluter
case behind the diluters.
Figure 9.201:VCC-Dilback
Figure 9.202:VCC-Dilback
Installation
1. Verify the instrument is switched off and the power cable
is disconnected.
2. Ensure the BUS END jumper is present; it is located on the
rear of the board at the lower-left corner.
3. Install in reverse order of removal.
Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• the following M&D checks:
– 50 µL Dispense Test
– 800 µL Dispense Test
See Section 5, Maintenance and Diagnostic Procedures,
for more information.
Level Sensors
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
Overview
There are three unique level sensors on the instrument:
• system liquid
• system waste
• solid waste
Removal
1. Switch off the instrument.
2. Disconnect the sensor in question from the SMIO board
• system liquid (J18)
• system waste (J19)
• solid waste (J21)
Liquid waste
sensor
System liquid
sensor
Installation
1. Install the sensor onto the bracket and secure with two
screws.
2. Route the cable up through the hole in the cabinet,
between the instrument and cabinet, over to the right
access door, and up to the SMIO board.
3. Plug the sensor cable into the appropriate connector.
• The system liquid sensor cable is labeled in green.
• The liquid waste sensor cable is labeled in red.
Figure 9.206:SMIO-SAFY
Door Locks
Removal
1. Switch off the instrument.
2. Open the front access panel and front worktable cover.
3. Loosen one screw (M3) securing the door lock.
4. Disconnect the cable at the door lock or disconnect the
door lock cable at the SMIO-SAFY board.
Installation
1. Open the front access panel.
2. Insert the door lock into position.
3. Finger tighten the socket head screw.
4. Connect the cable at the door lock or at the SMIO-SAFY
board.
• J5/J24 left door lock
• J6/J23 right door lock
5. Align the door lock to the catch so the door closes
smoothly.
6. Tighten the screw on the door lock.
7. Arrange the cables in the duct so they are not bent.
8. Close the front access panel.
Verification
Verification is performed through the S&S software. Refer to
Check Door Locks on page 4–115.
1 mL Subsystem Assembly
CAUTION: Hot Surface. Heater zone 1 operates at a
temperature of 50°C and heater zone 2 operates at
75°C. Be careful of exposure to hot surfaces.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
Removal
1. Switch off the instrument.
2. Disconnect the two 1 mL Subsystem cables from the 1 mL
Subsystem.
Installation
1. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
2. Connect the two cables to the two heater boards, accessed
through the notched opening in the bottom cover of the
1 mL Subsystem.
Removal
1. Switch off the instrument.
2. Remove the 1 mL Subsystem from the worktable.
3. Remove the racks from the 1 mL Subsystem.
4. Turn the 1 mL Subsystem upside down and place it on the
bench.
5. Remove the eighteen outer and four inner screws (M2)
that secure the bottom cover in place.
Installation
1. Place the new heater block in the 1 mL Subsystem.
Verification
Perform the 1 mL Subsystem Heater Block Temperature check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.
Removal
1. Switch off the instrument.
2. Remove the 1 mL Subsystem from the worktable.
3. Remove the racks from the 1 mL Subsystem.
4. Turn the 1 mL Subsystem upside down and place it on the
bench.
5. Remove the eighteen outer and four inner screws (M2)
that secure the bottom cover in place.
Installation
1. Ensure the board address is set to match the replaced
board. The address will be either 3 or 4.
2. Place the heater board assembly into the 1 mL Subsystem
and connect the two cables linking the heater blocks to
the heater boards.
3. Secure the heater board assembly to the side wall of the
1 mL Subsystem with four screws.
4. Install the bottom cover with four longer inner cover
screws and eighteen shorter perimeter screws.
5. Place the rack back into the 1 mL Subsystem.
6. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
7. Connect the two 1 mL Subsystem cables originating from
the OPTIBO board to the 1 mL Subsystem.
Verification
Perform the 1 mL Subsystem Heater Block Temperature Check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.
Removal
1. Switch off the instrument.
2. Remove the 1 mL Subsystem from the worktable.
3. Remove the racks from the 1 mL Subsystem.
4. Unscrew and remove the bottom cover as previously
described.
5. Disconnect the heater cable from the heater block and
heater board.
Installation
1. Connect the heater cable from the heater block to the
heater board.
2. Verify the black heater cables are placed in the notches in
the plastic dividers. This ensures the cables are not
pinched when the bottom cover is installed.
3. Install the bottom cover as previously described.
4. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
5. Connect the two 1 mL Subsystem cables originating from
the OPTIBO board to the 1 mL Subsystem.
Verification
Perform the 1 mL Subsystem Heater Block Temperature Check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.
Removal
1. Switch off the instrument.
2. Open the left access service door and disconnect the two
cables from CAN OPTION 3 and 4 on the OPTIBO board.
Installation
1. Route the 1 mL Subsystem cables through the notched
holes in the sealing strip, leaving enough length to
connect to the 1 mL Subsystem.
2. Route the cables underneath the worktable surface, along
the front edge of the FaWa assembly, and up to the left
access door.
Verification
Perform the 1 mL Subsystem Heater Block Temperature check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.
Chiller/Output Assembly
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of the system modules, and
carriers and racks on worktables. When operating the
instrument, it could be necessary to run the test with
the instrument’s safety panels open. Keep body parts
away from moving parts of the instrument (e.g., LiHa,
RoMa, tips, and gripper fingers) during setup and test
procedures.
Removal
1. Switch off the instrument.
2. Switch off the chiller using the on/off switch on the back
of the chiller, and disconnect the RS-232 communication
cable and the power supply cable from the back of the
chiller assembly
Installation
1. Install the Output Deck on grid locations 35 - 41. Ensure
that the cooling assembly engages the deck pins securely.
2. Plug the RS-232 and power supply cable into their
respective mating connectors on the chiller assembly.
3. Turn the chiller assembly on using the on/off switch on
the back of the chiller.
Verification
1. Turn the chiller on.
2. Log into the system as FSE.
3. Perform the M&D Chiller and Heater Temperature Check
on page 5–52. See Section 5, Maintenance and Diagnostic
Procedures for more information
Hood Maintenance
This section is applicable to hooded instruments only. Most
hood maintenance is performed using the latest revision of the
hood repair ISA, which includes procedures for all applicable
field replaceable parts.
BOARD TEST
CU-Board
OPTIBO
CU-Board
CU-Board PosID V2
OPTIBO
CC MAGS #3
MPO
SMIO
SMIO SAFY
SYSTEM CAN
DILBACK Yes J10 (not labeled)
LiHa Yes J14
Backplane
CU Board No J1
OPTIBO No SW1 off
OPTION CAN
CU Board No J22
PosID 2 No J6/J8
OPTIBO No SW2 off
CC MAGS #3 No no jumper
CC MAGS #4 No no jumper
MPO No J2
RoMa Yes J2
Backplane
SMIO Yes J9 outer two pins
SMIO/SAFY No J9 inner two pins
Movement Check
Manually check movement of all (system) modules.
Return of Parts
Return of Parts to Abbott Molecular
Before an Abbott FSE performs any work on the instrument, or
before the instrument or any part is returned to Abbott, the
owner of the instrument must confirm in writing that
decontamination was performed properly and in accordance
with good laboratory practice guidelines. For this, the owner
must enclose a Decontamination Form. Refer to the Field
Decontamination Checklist on page 2–15.
Service Checklist
This form is a test requirement checklist for the FSE. Refer to
the m2000sp Service Manual for additional tests that might
be applicable for a specific replaced assembly or component.
Parts/Components Replaced
Z-Calibration Check
RoMa Mech. Adjust
Random Move Test
PosID Adjustment
FaWa Pump Test
Tightness Check
OPTIBO PCB X X X X X X X X
Power Supply X X
LiHa Arm X X X X X X X X
LiHa Y-motors X X X
ILID Cable X
Tip Adapters X X X X X
RoMa Arm X X X X X X X
RoMa Grippers X X X
Chiller X X
Parts/Components Replaced
Z-Calibration Check
RoMa Mech. Adjust
Random Move Test
PosID Adjustment
FaWa Pump Test
Tightness Check
RoMa Y, Z Motors X X X X X X
RoMa Z-brake X X X X X X
RoMa CU PCB X X X X X X
PosID X X X X
X-DC-Servo PCB X X X
No Tube Sensor X X X
Dilutor X X X X X
Syringe X X X
3-way Valve X X X X
Pipetting Tubing X X X X
Interconnecting Tubing X X X X
Aspirating Tubing X X X X
Parts/Components Replaced
Z-Calibration Check
RoMa Mech. Adjust
Random Move Test
PosID Adjustment
FaWa Pump Test
Tightness Check
Pressure-Relief Valve X X
Worktable X X X X X
Door Locks X
SMIO/SAFY X X
SMIO X X
1 mL Subsystem Assy X X X X
Solenoid X
DC-DCPower Board X
SECTION 10:
SHUTDOWN, STORAGE,
AND SHIPPING
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-4
When to Decontaminate . . . . . . . . . . . . . . . . . . . . . . . . . . 10-5
Decontamination Method . . . . . . . . . . . . . . . . . . . . . . . . . 10-6
Instrument and Cabinet . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-7
SCC and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-13
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-14
When to Decontaminate
In addition to regular decontamination, the user must
thoroughly decontaminate the instrument according to
standard laboratory regulations in the following cases:
• before any maintenance or service is performed on the
instrument
• before an FSE performs on-site service on the instrument
• before the instrument is returned (e.g., for repair)
• prior to storage of the instrument
• prior to disposal of the instrument or parts of it
• in most cases, before the instrument or individual parts
leave the user’s site
Decontamination Method
The decontamination method must be adapted to the
respective application and associated substances used by the
operator. The owner assumes full responsibility for appropriate
decontamination of the entire instrument.
Refer to the m2000sp Operations Manual for specific
decontamination instructions, and refer to the Field
Decontamination Checklist on page 2–15 of Section 2 in this
manual.
WARNING: Potential Biohazard. Contamination
hazards exist if parts of the instrument are not
completely decontaminated. Components with direct
contact (or the potential for direct or accidental
contact) with chemical or biological material must be
treated, including the tubing system and all upstream
equipment.
17. Install the left and right support brackets using two screws
to secure each bracket.
20. Carefully move the LiHa and RoMa arms to the left.
21. Support the LiHa and RoMa with styrofoam pieces.
23. With the assistance of others, carefully lift the m2000sp off
the cabinet and place it on a pallet. Use the lifting handles
to lift the m2000sp. Follow the instructions in Lifting the
Instrument on page 4–22 for proper use of the lifting
handles.
WARNING: The instrument is heavy. Four people
are required to carefully lift the instrument onto
the cabinet. While lifting, ensure the lift handles
remain engaged with the locking features of the
instrument.
Storage
• Use a cover to protect the instrument against dust and
debris, and store the instrument under the following
conditions:
– Storage Temperature: 1 to 60°C/34 to 140°F
– Storage Humidity: 5 to 80% relative (non-condensing)
at 울 30°C/86°F
• Store the instrument in its original packaging. Store all
manuals and logbooks with the instrument.
NOTES
SECTION 11:
GENESIS SETUP AND
SERVICE SOFTWARE
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-7
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-8
PC Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-8
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-8
Firmware Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-9
Setup & Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-10
Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-10
Starting and Finishing Setup and Service . . . . . . . . . . 11-10
Password Protection . . . . . . . . . . . . . . . . . . . . . . . . . . 11-11
Setup & Service Main Window . . . . . . . . . . . . . . . . . . 11-12
Standard Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-14
Move Pages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-17
Operating Conditions . . . . . . . . . . . . . . . . . . . . . . . . . 11-21
Normal/Simulation Mode . . . . . . . . . . . . . . . . . . . . . . 11-22
System Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-23
Password . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-24
COM Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-25
Initialize Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . 11-26
Load Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-26
Test Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-28
Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-29
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-30
Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-32
Print Specific Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-32
Exit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-33
View and Tools Functions . . . . . . . . . . . . . . . . . . . . . . . . 11-34
View Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-34
Instrument Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-35
Instrument Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-35
Basic Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-35
Serial Number Settings. . . . . . . . . . . . . . . . . . . . . . . . . 11-39
Firmware Download . . . . . . . . . . . . . . . . . . . . . . . . . . 11-41
EEPROM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-42
Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-44
Set X-drive Properties . . . . . . . . . . . . . . . . . . . . . . . . . 11-46
Set Machine Size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-47
Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-48
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-50
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-51
Command Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-52
Batch File Execution . . . . . . . . . . . . . . . . . . . . . . . . . . 11-53
Command Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-53
Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-57
Results Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-58
Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-62
Version Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-63
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-64
System Devices Functions . . . . . . . . . . . . . . . . . . . . . . . . 11-67
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-67
System Devices Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-68
Positive Identification Functions for PosID 2 . . . . . . . . . 11-69
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-69
Mechanical Adjustment . . . . . . . . . . . . . . . . . . . . . . . 11-72
Bar Code Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-88
Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-91
Movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-101
Liquid Handling Arm (LiHa) . . . . . . . . . . . . . . . . . . . . . 11-103
LiHa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-104
Movement Axes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-105
Required Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-105
Before Starting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-105
Panel Description . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-106
Procedures and User Permission . . . . . . . . . . . . . . . . 11-107
Directory, Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-107
LiHa Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-108
Channel Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-110
Move LiHa Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-114
Setup Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-116
Set Default Parameters. . . . . . . . . . . . . . . . . . . . . . . . 11-118
Determine Range . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-118
Determine Reference Positions . . . . . . . . . . . . . . . . . 11-121
Determine Spacing . . . . . . . . . . . . . . . . . . . . . . . . . . 11-123
Individual Z Alignment . . . . . . . . . . . . . . . . . . . . . . . 11-124
Check Tip Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . 11-124
Calibrate the Lower DiTi Eject Protocol . . . . . . . . . . 11-125
Dilutor Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-126
Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-133
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-135
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-136
Move Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-138
Range Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-140
Random Move Test . . . . . . . . . . . . . . . . . . . . . . . . . . 11-141
Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-143
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-146
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-148
Robotic Manipulator Arm (RoMa) . . . . . . . . . . . . . . . . . 11-149
RoMa Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-155
Introduction
PC Requirements
Genesis Instrument software must be installed on a target
computer which is or will be connected to an m2000sp
instrument. The installation of the Genesis Setup and Service
software occurs during the installation of system software.
Installation
Refer to the latest software TSB and/or ISA for installation
instructions.
Firmware Compatibility
The Genesis Instrument software, Setup & Service was
validated with the firmware versions as defined in the
following table.
The following table lists firmware versions that were valid
when this manual was issued. It is possible some have
since changed. Contact your Abbott Representative for
the latest firmware list.
Table 11.1: Firmware Versions
File name
Device Download Firmware Comments
(--- = no file)
Genesis-CU Yes v1.63-12/2002 GENI163.hex Controls LiHa and
Control Unit machine
MPO Yes v2.00-11/99 MPO200.hex FaWa
Monitored Pump
Option
PosID 2 Yes v2.50-02/03 POS2_250.hex PosID 2
Positive
Identification
RoMa Yes v1.13-07/2002 dcuro113.hex RoMa on Device-CU
Robotic
Manipulator Arm
SAFY Yes v1.00-05/2001 ___sa100.hex SAFY
SMIO-SAFY Yes v1.01-11/99 SMIO101.hex SMIO-SAFY
VCC Yes v1.20-06/2000 vcc120.hex (for Dilutor type XP
Volume Control 3000 plus)
Center
MAGS Yes v1.30-01/2003 MAGS for 1 mL Subsystem
130.hex
Password Protection
Purpose
Access to the FSE level of Setup & Service is password protected
to prevent unauthorized use of the Setup & Service module of
the Genesis Instrument software.
Access Levels
There are two access levels:
• Operators—Operators can perform tests and validation
procedure without password entry. If so, some menu items
and controls are disabled. Operators cannot perform setup
and calibration procedures that could affect instrument
performance if performed incorrectly.
• FSEs—Setup and calibration procedures require the FSE
password because of restricted access. In this manual,
these procedures include the following note:
Overview
The Genesis Setup & Service Main window is divided into
four sections as illustrated below.
Worktable (empty)
Menu Bar
The menu bar includes the following menus and commands.
Command Description
System System functions
View View functions
Instrument Instrument functions
System System devices
Devices
Liquid System Liquid system functions
Options Options 1 and 2, and Te-MO
Toolbar
The toolbar contains quick-access buttons for the most
frequently used functions.
1) 2) 3) 4) 5) 6)
Button Description
1) <System><Password>
2) <System><Initialize Instrument>
3) <System><Load Worktable>
4) <System><Test Configuration>
5) <System><About>
6) <<System><iew><Worktable><Zoom Active>
Standard Panel
Description
Many Setup & Service functions open a function panel that is
subdivided into several pages. The following illustration
displays the Access Status panel.
Device Selection
Available devices listed below are included in the Available
Devices section on the corresponding Contents page. The
behavior of the software depends on the number of connected
devices.
No of
Connected Behavior
Devices
0 The Start button is dimmed and the tabs are
visible.
1 The connected device is automatically
selected and its settings are read
automatically.
2 or more None of the connected devices are selected.
One device must be selected then its settings
are read.
Command Buttons
The command buttons near the bottom of the screen are used
to perform the desired action.
Button Description
Start Starts the desired procedure. This is possible:
- if a device is selected in the Available Devices
section.
- if at least one setup or test procedure is selected.
- if valid data were entered on the corresponding
pages.
- If more than one procedure is selected, they are
executed in the sequence as listed in the
Contents page. The topmost procedure is
executed first. After starting a procedure, Start
changes to Stop.
Stop Stops a running procedure. The procedures
executed are marked gray, those not executed are
black.
Execution can be resumed with Start if no settings
are changed.
Print Prints the test results or the Test report, if
implemented.
Save As Starts the standard Windows Save As dialog to
save the test configuration data in an appropriate
*.any file.
Enter the full file name (with extension.any).
Default Sets all parameters to their default values.
Undo Reverts to the previous settings.
OK Saves the defined values and closes the panel.
Cancel Closes the panel, but does not save any changes.
Close Closes the panel.
Apply Saves defined values, but does not close the panel.
The window remains open. After selecting Apply,
Cancel changes to Close and OK is dimmed.
Move Pages
Description
Panels for some system devices and options that contain
movable parts (e.g., LiHa, RoMa) contain a Move page. The
Move page allows movement of the whole device or parts of it
in certain movement axes. The following example illustrates
the Move LiHa page.
NOTE: In this section, only the movement buttons are
described. For other controls on a specific Move page,
refer to the description of the respective system device
or option.
Movement Buttons
Depending on the parts that can be moved, a Move page
includes some or all of the following movement buttons.
Button Description
Single step, in 1/10 mm or 1/10 degree,
movement/rotation buttons.
Ten steps, in 1 mm or 1 degree,
movement/rotation buttons.
Button Description
Continuous movement/rotation buttons
when button is kept pressed.
Special buttons to move certain parts to
specific logical positions. Refer to the Move
page of the corresponding device for
details.
Insert Home
Page Num
/ _
Up lock *
Delete End
Page 7 8 9
Down Home PgUp
+
4 5 6
1 2 3
End PgDn
Enter
0 .
Ins Del
Directions
The following table lists the axes and directions in which the
devices (LiHa and RoMa) can be moved.
Key in
Key (Left of Movement/
Numeric Pad Movement
Numeric Rotation Axes
(Num Lock Axes LiHa
Keypad) RoMa
Switched on)
6 X+ (right) X+ (right)
4 X- (left) X- (left)
2↓ Y+ front Y+ front
8 Y- (rear) Y- (rear)
Page Down PgDn 3 Z+ (down) Z+ (down)
Page Up PgUp 9 Z- (up) Z- (up)
a
Home 7 Home R+ (CW) ---
End 1 End R- (CCW) a) ---
b G+ (increase) c
Delete Delete G+ (open)
Insert 0 Insert G- (close) b) G- (decrease) c)
+ --- ---
- --- ---
a. RoMa - Rotation of gripper head clockwise/counterclockwise
b. RoMa - Open/close grippers
c. LiHa - Increase/decrease spacing between tips
Steps
Movable parts of the listed arms or options are moved as
described below.
• Each time one of the above keys is pressed, the part is
moved/rotated by one step, 0.1 mm or 0.1 degree.
• If the key is pressed and held down, the part is
moved/rotated continuously at a speed of two steps per
second.
• With LiHa and RoMa, the key can be pressed continuously
with the Ctrl key pressed at the same time. If so, the arm
is accelerated until it reaches a maximum speed.
Result Files
For newer functions, each time a test procedure is started, a
result file is created and test results (all information as on the
Test reports) are written to the directories listed.
Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Results
File name: <function>_<serial_number>_<date>_<ti
me>.any
Example: LiHa_2209_29Mar2003_09-14-18.any
Log Files
Some functions create a log file to which measured values are
written.
Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Log
File name: Function dependent, refer to corresponding
section
Operating Conditions
Before operating Setup & Service, perform the following
verifications.
1. Ensure the correct firmware is downloaded; refer to
Firmware Compatibility, page 11-9.
2. To avoid movement crashes, verify the instrument is set
up with <Instrument><Basic Setup>.
NOTE: The correct worktable size is set
automatically when a worktable is loaded.
3. Some functions require an empty worktable free of all
obstacles. Observe the NOTES included in the
corresponding sections.
4. Do not start any function if the prerequisites or
requirements described are not fulfilled.
Normal/Simulation Mode
Operating Modes
The instrument software can run in two operating modes. This
section describes the normal mode of operation.
Normal Mode
This is the normal mode of operation of Setup & Service
software for all Genesis Instruments and workstations, with all
functions and menu entries enabled.
Simulation Mode
In the simulation mode, Setup & Service software can be run
without a physical instrument connected to the computer.
This mode allows telephone support personnel to simulate
certain features when no instrument is connected to their
computers. However, access to features is quite restricted in
this mode; therefore its use is not recommended.
If an instrument is not connected to the computer, the Setup
& Service software automatically starts in simulation mode.
Normal and simulation modes are possible for all supported
instrument types.
System Functions
Description
The System menu includes all commands that apply to the
whole system and not to a particular function.
Commands
The System menu includes the following commands.
Command Description
RMP Enables operation in RMP mode; refer to
Operating Conditions, page 11-21. Not
applicable with the m2000sp instrument.
Password For entering the FSE password; refer to
Password, page 11-24.
Initialize Prepares the instrument for usage by
Instrument initializing each axis. After switching on the
instrument, it must be initialized.
Load Loads a predefined or edited worktable map;
Worktable refer to Load Worktable, page 11-26.
Test Loads an *.ini or *.any configuration file;
Configuration refer to Test Configuration, page 11-28.
Print Setup Starts the standard Windows dialog to set up
a printer.
Print Prints test configuration and test results;
refer to Print, page 11-29.
Command Description
About Opens a window that displays copyright,
version, and other information related to
the Setup & Service software.
Exit Terminates the Setup & Service software;
refer to Exit, page 11-33.
Password
Purpose
To prevent unauthorized use of the Setup & Service module of
the Genesis Instrument software, access to the FSE level of
Setup & Service software is password-protected. No other
passwords are required. The FSE password is always the same
because there is not an available function to change the
password.
Procedure
Perform the following steps to enter the FSE password.
1. Start the function with <System><Password> or select the
icon on the toolbar. The Password dialog displays.
COM Port
Purpose
This function allows the communication port (e.g., COM 1, 2,
etc.) to be selected or changed. The computer communicates
with the instrument through the communication port.
NOTE: For m2000sp, the communication port should
always be set to COM1.
Procedure
Perform the following steps to select or change a
communication port.
1. Ensure the instrument is connected to the required COM
port connector, usually located on the rear side of the
computer. Consult the accompanying documentation of
the computer, if necessary.
Initialize Instrument
Purpose
This function initializes the instrument. During initialization,
all installed system devices (PosID, LiHa, and RoMa) are
moved to their initial positions:
• PosID RoMa and LiHa to the left side.
Procedure
Perform the following step to initialize the instrument.
1. Start the function with <System><Initialize Instrument>
or select the icon in the toolbar. The following message
displays:
Load Worktable
Purpose
To perform setups and tests, Setup & Service software requires
information about the location of carriers, racks, etc. on the
worktable. Tecan provides five predefined worktable maps
stored in the Genesis software database:
• NAT Worktable
– use for all m2000sp applications
• Default Worktable
• Empty Worktable
• Field Worktable NPS
• Liquid System Check Worktable
Additionally, Abbott provides the m2000sp worktable.
Procedure
Perform the following steps to load a worktable map.
1. Start the function with <System><Load Worktable> or
select the icon in the toolbar. The Select Worktable
dialog displays.
Test Configuration
Purpose
The Test Configuration function loads a *.any or *.ini
configuration file.
NOTE: Never alter configuration files. They contain
configuration specific data to perform all instrument
tests.
NOTE: For the m2000sp instrument it is not necessary
to load the Service.ini file because the file is loaded by
default.
Procedure
Perform the following steps to load a *.any or *.ini file.
1. Start the function with <System><Test Configuration>
or select the icon in the toolbar. The Open
Configuration dialog displays.
2. To load an *.any file, select the file type in the Files of
type list.
3. Select the appropriate file.
4. Load the file with Open.
• *.ini configuration files are normally located in the
C:\Program Files\Tecan\Genesis Instrument
Software\Service directory.
• *.any configuration files are saved in a user-defined
directory.
Print Sub-menu
The Print sub-menu includes the following sub-menu
commands.
Command Description
Configuration Prints the configuration data stored in the
appropriate *.ini file; refer to Configuration,
page 11-30.
Test Prints test results of tests performed with the
instrument; refer to Test, page 11-32.
Configuration
Purpose
This function prints the configuration data stored in the
appropriate *.ini file. It opens the dialog displayed on the left.
The Common Print Information dialog includes the
following controls.
Control Description
Destination
Printer Checkbox to select the printer for output
destination.
File Checkbox to select a file for output
destination.
After selecting OK, the standard Windows
Save As dialog displays. Select the desired
folder and enter a file name. Save the
output with Save.
Header Fields for free text. The entries are not
Information checked against a database.
Operator Operator ID for header information.
Option x Option serial number x of the devices
Serial No currently used, e.g., the serial number of a
replaced device.
OK Starts printing.
Cancel Aborts the function without printing.
Procedure
Perform the following steps to print all configuration data.
1. Start with <System><Print><Configuration>. The
Common Print Information dialog displays.
2. Select the print destination(s) and enter the Header
Information.
3. Start immediate printing with OK.
Test
Purpose
This function prints the results of tests performed with the
instrument.
Procedure
Perform the following steps to print the results of tests
performed with the instrument.
1. Start with <System><Print><Test>. The Common Print
Information dialog displays.
2. Select the print destination(s) and enter the Header
Information. Refer to Configuration, page 11-30.
3. Start immediate printing with OK.
Control Description
Print
Configuration Checkbox to select the configuration
data to be printed.
Detailed Checkbox to select detailed test
Results results to be printed. If the Detailed
Results checkbox is not selected, the
results printed will only be Test
passed or Test failed.
Comments Field for free comments.
OK Starts printing.
Cancel Aborts the function without
printing.
Help Provides help.
Exit
Perform the following steps to finish the Setup & Service
software safely without data loss.
Select <System><Exit>.
Because changes to setup or test configurations are applied
immediately when selecting Apply or OK, there is no need for
<File><Save> or similar function in the Setup & Service
software.
Command Description
Worktable Refer to Figure 11.8 below.
Tool Bar Displays/hides the toolbar of the Main window.
Status Bar Displays/hides the status bar at the bottom of
the Main window.
Command Description
Carrier Displays/hides carrier names in the worktable
Names map.
Rack Displays/hides rack names in the worktable
Names map.
Zoom Zooms the worktable map.
Active
Instrument Functions
Instrument Menu
The Instrument menu includes the following commands.
Command Description
Basic Setup Sets up an instrument (initial setup); refer to
Basic Setup, page 11-35.
Command Allows for use of single commands or
Tool execution of batch files.
Information Provides detailed information about
installed system devices and options; refer
to Information, page 11-57.
Basic Setup
Purpose
The Basic Setup panel allows setup of the m2000sp instrument
for further use (initial setup). This setup must be performed in
the following cases:
• To set X-drive properties, e.g., changing a CU board
without restoring parameters stored in EEPROM.
• When new firmware will be downloaded.
• To backup or restore parameters stored in EEPROM.
• When serial numbers will be configured.
• To define possible cutouts in the worktable.
Directory, Files
The Basic Setup function can create the following files (refer
also to Setup & Service, page 11-10).
Description
Saved in C:\Program Files\Tecan\Genesis
directory Instrument Software\Results
File name BasicSetup_<serial_number>_<date>_<tim
e>.any
Pages
The Instrument Basic Setup Panel is subdivided into the
following pages.
Page Description
Contents General overview, procedure selection.
Serial Number Serial number data; refer to Serial Number
Settings Settings, page 11-39.
Firmware Configuration of the firmware to be
Download downloaded; refer to Firmware Download,
page 11-41.
EEPROM Backs up/restores contents of EEPROM; refer
to EEPROM, page 11-42.
Worktable Provides information about the worktable
layout; refer to Worktable, page 11-44.
Set X-drive For setting up the X-drive; refer to Set
Properties X-drive Properties, page 11-46.
Information Displays instrument properties, diagnostic
data, and device information; refer to
Information, page 11-48.
Printing Print selection for the Test report; refer to
Printing, page 11-50.
Controls
The Contents page includes several checkboxes for selection
(or omission) of the following procedures,
Checkbox Description
Serial Number Displays the serial number of all installed
Settings devices; refer to Serial Number Settings,
page 11-39.
Firmware Displays and downloads firmware for
Download installed devices; refer to Serial Number
Settings, page 11-39.
EEPROM Backs up/restores EEPROM parameters; refer
to Serial Number Settings, page 11-39.
Set X Drive Determines the order of the arms and arm
Properties type at each position, and sets the default
offset and displacement for each arm; refer
to Serial Number Settings, page 11-39.
Checkbox Description
Set Machine Determines the size of the instrument; refer
Size to Serial Number Settings, page 11-39.
Purpose
The Serial Number Settings procedure displays serial numbers
for the installed devices. If no serial number is set for a device
(exception), it can also be entered.
Procedure
Perform the following steps to display serial numbers.
1. Select the Serial Number Settings checkbox (聺) and
switch to the Serial Number Settings page.
Control Description
Set for Checkboxes to select device addresses for
which the serial number will be set.
Firmware Download
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Firmware Download procedure displays the current
firmware versions for the installed devices and downloads a
new or updated firmware version to the instrument.
Before starting the procedure refer to Firmware Compatibility
in this section to verify firmware compatibility.
Procedure
Perform the following steps to display the current firmware
version and to download a new one.
1. On the Contents page, select the Firmware Download
checkbox (聺) and switch to the Firmware Download
page.
EEPROM
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The EEPROM function allows backup on disk of EEPROM
parameters of one or more devices, and/or restore such
parameters from disk. For example, backup of current device
parameters is recommended if an electronic board is replaced.
After the board is replaced, parameters can be restored so setup
or calibration procedures need not be performed again.
Control Description
Do for Checkboxes to select the device addresses of
the EEPROMs to backup.
Control Description
Restore with Displays the names of the files selected with
the Browse […] buttons.
Browse […] Command buttons that start the standard
Windows Open dialog to search the files with
parameters to restore.
Backup
1. On the Contents page, select the EEPROM
Backup/Restore checkbox (聺) and switch to the
EEPROM page. The EEPROM page displays.
Restore
Perform the following steps to restore the contents of a file
previously backed up.
Worktable
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Worktable page is a tool that allows definition of possible
cutouts positions in worktables of the following instruments:
• m2000sp instrument
• Cutout left: none
• Cutout right: none
Control Description
Upper part Picture that illustrates the worktable.
Selected cutouts display in the picture.
Frame Cutout Set of option buttons for definition of the
left cutout type on the left machine side.
None No cutout on this side.
Centrifuge Cutout for centrifuge below worktable.
Reader Cutout for reader below worktable.
Frame Cutout Set of option buttons for definition of the
right cutout type on the right machine side.
Refer above.
Frame Cutout Checkbox to select a cutout at the right
border edge of the worktable.
Procedure
Perform the following steps to define cutouts in the
Worktable.
1. From the Main menu, select <Instrument><Basic Setup>.
2. If not previously done, set the machine size; refer to Set
Machine Size, page 11-47.
3. Select the Worktable tab. The Worktable page displays.
4. Use the option buttons and/or checkboxes to define the
m2000sp worktable.
• Cutout left: none
• Cutout right: none
• Cutout board: not selected
5. Select the appropriate command button to store or discard
the selections:
• Apply: selections are stored, the panel remains open.
• OK: selections are stored, the panel is closed.
• Cancel: discards selections and closes the panel.
Purpose
The Set X Drive Properties procedure determines the order of
the arms and arm type at each position, and sets the default
offset and displacement for each arm.
Before Starting
• Ensure the worktable is empty and free of obstacles at all
times.
• Verify each arm can be initialized.
• If the X-drive properties are set, the LiHa and RoMa setup
function must be performed afterward.
Procedure
Perform the following steps to set the X-drive properties.
1. On the Contents page, select the Set X Drive Properties
checkbox (聺). No other parameters are selected.
2. Start the procedure with Start.
3. At the end of the procedure, the values found are written
to the instrument.
Purpose
The Set Machine Size procedure detects the instrument size or
worktable length.
Procedure
Perform the following steps to determine the machine size.
1. On the Contents page, select the Set Machine Size
checkbox (聺). No further parameters need to be defined.
2. Start the procedure with Start.
3. At the end of the procedure, the values found are written
to the instrument.
Information
Purpose
The Information page summarizes current instrument
properties, diagnostics data, and device information.
Description
Type Instrument type, e.g., Classic, Freedom
Instrument
Properties
Size Instrument size—100, 150, or 200 [cm]
Pos Position of arm installed—left to right
Arm Arm installed
Addr Address of arm
Power Ups Number of times the instrument was
switched on since first startupa
On Time Total power on time in hours
Diagnostics
Printing
Purpose
The Printing page allows configuration of reports to meet
laboratory requirements.
Controls
The Printing page includes the following controls.
Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Instrument Checkbox to select instrument
Properties properties according to the
Information page.
Diagnostics data Checkbox to select the diagnostics
data displayed on the Information
page.
Multiple existing Data selection valid for all device data
Sections selected in the Detailed Print Section
Configuration section.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Allows preview of the report.
Control Description
Printer Setup Starts the standard Windows dialog to
set up a printer.
Use Paper Print in selected orientation.
Orientation of
Document
Print a Report
Perform the following steps to configure and print a report.
1. Switch to the Printing page (Figure 11.9).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the report onscreen with Result Preview.
4. Print the Test report with Print.
Test Report
Depending on selections, the Test report contains the
following data.
Header
• Global data such as software versions, instrument serial
numbers, date of creation and global test review (not
relevant)
• Signature section with operator name
Instrument Properties
Type, size, left arm, middle arm, right arm
Diagnostics Data
RS-232 host: error interrupts, repeated messages
Device Information
List which displays the following parameters for each
connected device: device name, address, firmware
version, bootware version, serial number
Command Tool
The Command tool allows input of single line and batch file
commands for troubleshooting.
The tool, for experienced Field Service Engineers, developers,
and the Tecan production department, provides the following
functions:
• Edit and execute single commands.
• Write, edit, and execute small batch programs (command
sequences). Such batch programs can be stored in a
directory and reloaded at a later time.
• These single commands and/or batch programs allow for
performance of special test sequences not accessible
through the normal menu.
Password Protection: Functions of the Command
Tool panel are password-protected and require the use
of the appropriate password.
Control Description
File Name Name of the currently loaded batch file.
Edit Window The large window is used to enter single
commands, that together compose a
command sequence. Commands can be
typed directly in the window. Each
command line must be terminated with the
Enter key.
Command Buttons
Command Description
Run All Select to execute all commands of the batch.
Stop Stops the execution of a batch.
Single Step Allows execution of the batch stepwise,
command line by command line.
Command Description
Resume Resumes the execution of a stopped batch,
starting at the current command line.
Reset Resets the internal logic after errors.
Load... Starts the Windows Open dialog where a
batch file containing a command sequence
can be selected.
Save Saves the current contents of the Edit
window.
Save As Starts the Windows Save As dialog where
contents of the Edit window are saved with
a different name.
Read Only Enables/disables write protection of batch
files. If this checkbox is selected, batch files
can be loaded from disk but not
overwritten.
Command Text field which displays the currently
executed command.
Response Text field which displays the response of the
instrument.
Single Commands
Select the Single Commands tab. The Single Commands
page displays.
This page allows input of single command lines, a view of
instrument response, and the answer to the command.
Control Description
Previous Displays previously requested single
command list: command lines. These commands can be
executed again later.
Timeout: The amount of time, in ms (milliseconds),
allowed for execution of the command.
Command: The single command line to be executed.
Answer: The response of the instrument to the single
command line.
Transmit Sends the command to the instrument and
executes the command.
• Select Transmit.
• The instrument attempts to execute the
command.
If the command was executed successfully, the appropriate
response displays in the Command: field.
Information
Purpose
The Information panel allows access of detailed information
regarding currently installed system devices and options
through a single panel. With this panel, screens or panels of
each individual system device or module are not opened to
obtain the required information.
No setups or tests can be performed through the Information
panel. Information panel functions are available to all users,
with no password required.
Pages
The Information panel includes the following four pages;
refer to the figures in the following paragraphs.
Page Description
Results Provides an overview of available test
results, grouped together by selectable
criteria.
Information Provides general information, parameters
and diagnostics data of installed device(s).
The displayed information depends on the
device type.
Version Provides information about the versions of
the installed system modules and panels.
Printing Configure and print the information
selected on the Results, Information or
Version pages.
Procedure
To access the Information panel: from the Main menu, select
<Instrument><Information>.
Results Page
Purpose
The Results page provides an overview of the stored test
results and helps to quickly locate results of a particular test.
Selection Criteria
The module name, serial number, date, and time are used as
selection criteria on the Results page.
1st Selection
2nd Selection
3rd Selection
4th Selection
1st Selection
2nd Selection
3rd Selection
4th Selection
Controls
The Results page includes the following controls.
Control Description
Upper-left list Tree view with four selection levels. It serves
box as a browser for quick selection of an
individual test. The nodes (small square
boxes with + or - sign) can be expanded or
collapsed to show or hide associated
sub-selections.
Right list box Lists available results for the item selected in
the left list.
List at bottom Definition or edit of the selection order.
OK Closes the panel.
Control Description
Cancel Closes the panel.
Print a Prints the results of the item(s) selected in
the right list.
a. Switch to the Printing page to print the results more
selectively.
Example
There are several available options to view results of all tests
run on a specific day. For example:
• Date criterion can be moved to the top of the list and
Time criterion to the second position.
• Serial Number criterion can be moved to the bottom of
the list and Module criterion to the third position.
The screen below illustrates an example of a Results page with
results grouped by date.
Information Page
Purpose
The Information page allows selection and viewing of
detailed information regarding the entire instrument, a system
device, or an option. The type of information provided
depends on the selected item.
Controls
The Information page includes the following controls.
Control Description
Refresh Loads the current device and module
information, and sets the Sample Time to
the current time.
Sample Time Time when the device and module
information was last read.
Upper list Tree view of the devices or modules from
which information can be retrieved.
Lower list Displays information about the selected
item in the upper list. The number of
columns and column headings in this list
depend on the selected item.
OK Closes the panel.
Control Description
Cancel Closes the panel.
Print a Prints the results of the item selected in the
right list.
a. Also, switch to the Printing page and print the results
more selectively.
Version Page
Purpose
This page displays the current versions of the installed system
modules and panels.
Controls
The Version page includes the following controls.
Control Description
Upper list Lists the system modules and displays the
following for each module: File name,
Version, and Description.
Lower list As above for the panels.
OK Closes the panel.
Cancel Closes the panel.
Print a Prints the results of the selected item.
a. Also, switch to the Printing page and print the results
more selectively.
Printing
Purpose
This page allows selective printing of information regarding
items selected on the Results page. This provides
configuration of a report that meets laboratory requirements.
Select the necessary components for inclusion in the report,
and omit those not needed.
Controls
The Printing page includes the following controls.
Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Further Depends on selections made on the Results
checkboxes page.
Multiple Data selection valid for all device data
existing selected on the other pages.
Sections
Operator Field for operator name. Refer to the
following note.
Comment Field for additional notes. Refer to the
following note.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to
setup a printer.
Printing a Report
Perform the following steps to print a report with the Printing
page.
1. Perform one of the following:
• Go to the Results page and select the result file(s) to
print.
Available Arms
The following robotic arms are installed on the m2000sp
instrument:
• Liquid Handling arm (LiHa) for liquid pipetting tasks.
• Robotic Manipulator arm (RoMa) Standard for plate
handling on and underneath the platform worktable.
Device Parameters
If an arm is set up, various device parameters were determined
and written to the device. Refer to the corresponding Results
page or Test report.
Reference Positions
m2000sp Instruments
There are a total of 45 grids on the m2000sp instrument. The
reference positions for the m2000sp are set at grids 1, 7, 22, 35,
41, and 45.
NOTE: Reference positions are defined separately for
each arm.
Command Description
PosID Sets up a Positive Identification system.
Refer to Positive Identification Functions for
PosID 2, page 11-69 for PosID 2.
LiHa Sets up a Liquid Handling arm; refer to
Liquid Handling Arm (LiHa), page 11-103.
Move Test Determines if the LiHa, RoMa, and PosID
can reach the whole range (Move Test, page
11-138), and when the Random Move test is
used. Determines if the mechanics of the
PosID, RoMa, and LiHa work properly.
RoMa Sets up a Robotic Manipulator arm; refer to
Move Test, page 11-138.
Purpose
The Positive Identification system (PosID) uses a bar code
scanner to read bar codes on carriers, racks, and containers on
both the primary side (e.g., sample tubes) and secondary side
(e.g., microplates).
PosID Type
Positive Identification is referred to as PosID 2 in the Genesis
Instrument software package. It is also referred to as PosID 2 in
this manual. This section describes the Setup & Service
functions for the PosID 2. The m2000sp instrument is
equipped with PosID 2.
Safety
WARNING: Class II Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing into the bar code laser beam or
reflections of the beam.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic discharge
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body prior to
touching the instrument.
Required Tools
The PosID Service Rack, illustrated below, is required for the
setup function of the PosID 2 system module.
Guideline
Calibration lines
Log File
Each time the test function is started, a log file is created to
which test results are written.
Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Service Log
File name: <date>_<ongoing_no>.log
Example: 00123102.log
Movement Axes
Movements of the PosID 2 are coordinated with other system
modules, such as the LiHa and RoMa. There are three
movement axes:
Range Movement
X movement Movement of PosID 2 left-right
Y movement Movement of PosID 2 gripper
forward-backward
X' movement Vertical rotation motion of PosID 2 scanner
head
B movement Horizontal rotational motion of PosID 2
scanner head
X X
Y
X
Command Description
Setup Set up PosID 2.
Bar code Set bar codes.
Types
Test Check PosID 2 functionality and settings.
Movement Allow movement of PosID 2.
Mechanical Adjustment
Purpose
Mechanical accuracy must be verified before the PosID 2 can
be set up with Genesis Instrument software.
NOTE: Ensure the worktable is empty and free of
obstacles at all times.
Fixing Screws
Release Finger
0.2mm
Carrier
Adjusting Slot
Carrier
0.5 mm
Change Position
The change position is the transition position in which the
scanner head goes from moving around its vertical B'-axis to
moving around its horizontal X'-axis, or vice versa. Figure
11.11.17 on page 11-74 and Figure 11.11.18 on page 11-74
illustrate the change position.
Defaulta Basicb
X-offset no default 780
Scale Factor no default 10000
Logical
Default a Basic b
Position
Y-offset no default 15
Load 1 2220 2220
Remove 2 3170 3170
Max Read 3 0 0
Connect 4 3170 3170
Push Connect 5 3170 3170
Carrier Present 6 3000 3010
Check
Init Position 7 2850 2850
Test Rack 8 3100 3099
Logical
Default a Basic b
Position
B-offset 80 80
Carrier Read 1 0 0
Position
Horizontal Read 2 2610 2650
Position (MTP)
Vertical Read 3 1350 1350
Position
(Sample/Reagent)
Collision Avoidance 4 0 0
Position
Turn Position 5 1410 1410
a. Default values set in the Setup Procedure by software (refer
to Figure 11.11.19 on page 11-78).
b. Basic values, used when PosID 2 is new (factory set)
Setup Procedure
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
This procedure is used to set up the PosID 2 with the aid of the
Setup & Service software.
Starting Setup
Perform the following steps to set up the PosID 2.
1. Start with <System Devices><PosID><Setup>. The PosID
2 Adjustment dialog (Figure 11.19) displays.
Adjustment Dialog
Adjustment Procedure
1. Select Set to perform the following:
• Reset the default B-offset and the logical positions of
the bar code scanner head for the B-axis. The PosID 2
moves through all five logical positions.
• Reset the logical positions of the gripper for the Y-axis.
The gripper moves through all eight possible logical
axes.
• Store results in EEPROM.
2. Select Run. The PosID 2 runs in all axes to the end
positions to determine its range.
Description
X List to select the movement step size,
in mm, in X-direction.
Left Moves the PosID 2 arm from right to left in
X-direction.
Description
Right Moves the PosID 2 arm from left to right in
X-direction.
Y List to select the movement step size,
in mm, in Y-direction.
Rear Moves the gripper from front to rear.
Front Moves the gripper from rear to front.
OK Confirms the adjustment.
onscreen Aborts the procedure without saving any
changes.
Guideline
Laser Beam
Correct Incorrect
Horizontal Calibration
Line
Laser Beam
Correct Incorrect
Figure 11.26: Vertical/Horizontal Scanner Head Adjustment
Control Description
Beam Angle List to select the rotation step size, in
degrees, in B-direction.
CCW Rotates the scanner head
counterclockwise.
Control Description
CW Rotates the scanner head clockwise.
OK Confirms the adjustment.
Cancel Aborts the procedure without saving any
changes.
Introduction
Default bar code settings are reloaded with each cycling of the
power. The PosID 2 is capable of simultaneously reading six
different bar codes in a run, although m2000sp only supports
five bar code types.
Start Stop
Bar code Check Digit
Bar code Type Character Active
Length Mode
Transmission
Code 2/5 2,4,6,8,....,20* ON with or No setting Yes
Interleaved without
transmission or
OFF
Code 39 1-20 ON with or No setting Yes
Standard without
transmission or
OFF
CODABAR flexible 1-20 ON with or ON with or Yes
without without
transmission or transmission or
OFF OFF
Code 128 flexible No setting No setting Yes
Code 93 flexible 1-20 No setting No setting Yes
Example
The following schematic shows a bar code of type Code 39
Standard. Information about bar codes can be found in
literature supplied by the bar code or test kit manufacturers,
respectively.
*1234567890123456N*
Stop character
Check digit
bar code
Start character
Test
Purpose
The Test function verifies proper functioning of the bar code
scanner.
To run the Test function, at least two carriers (e.g., tube rack,
microplate carrier, etc.) must be available. The carriers must
have correctly placed bar code labels.
Test Sequence
During the test, the carrier and container bar codes are read in
the following sequence:
• Carrier ID, and if desired, user ID bar code of all carriers
(Reference Read).
• Bar codes on every container on every carrier (Reference
Read).
• Steps 1 and 2 for the defined Number of cycles (Actual
Read).
Test Procedure
Perform the following steps to check functionality of the
PosID.
1. Start the function with <System Devices><PosID><Test>.
The PosID 2 Test Configuration dialog displays.
Control Description
Number of cycles Field for the number of Actual Reads
to be performed.
To pass the test, at least 5 cycles
must be performed.
Grid Positions Field to define the range from…to
where the PosID test will run.
Carries Codes Checkboxes to specify which bar
codes are read.
Read Carrier Carriers and containers.
Only Carrier Only carriers (no containers, e.g.,
ID tube, microplate, and trough bar
codes).
Read User ID User identifications.
Operator/Logfile Fields for operator name, PosID SN,
and log file.
Operator Field for operator name. An entry is
required.
Serial number Field for PosID serial number.
Logfile Field to define the log file directory.
An entry is required. The default
path is C:\Program
Files\Tecan\Genesis Instrument
Software\Service Log.
The log file name itself is based on
the date and an ongoing number,
e.g., 00123102.log, and contains a
summary of the test results.
Logfile Starts the standard Windows dialog
Directory to browse and find a directory for
the log file.
Run Opens an empty PosID 2 Test Results
window to start the test procedure.
Close Finishes the function and saves the defined
values.
Cancel Aborts the function without saving any
changes.
Control Description
Save Starts the standard Windows Save As dialog
to save the data in an appropriate *.ini file in
the C:\Program Files\Tecan\Genesis
Instrument Software\Service directory.
Print Prints the test results stored in the defined
log file. It is enabled only if a test was run.
More Opens the PosID 2 Test - Advanced
Configuration dialog for advanced
configuration; refer to Advanced
Configuration on page 11–98. For FSEs only
and requires the appropriate password.
Test Results
During the test, values in the PosID 2 Test Results window are
continuously updated.
Description
Heading Number of reads done/defined and worktable
Line position, for which results display in the table.
The position can be switched with the scroll
bar.
NT Reading result of the No Tube sensor during
the Reference Read.
T Tube found.
N No tube found.
Reference Bar codes read in the Reference Read.
Read *** No reading possible, but tube is placed
correctly.
$$$ No tube found in this position.
NT Reading result of the No Tube Sensor during
the Actual Reads.
Actual Read Bar codes read in the Actual Reads.
NT No Tube: no tube found in the read attempt.
NR No Read: no bar code(s) were read, but tubes
were placed correctly.
WR Wrong Read: bar code(s) read do not match bar
code(s) read in the Reference Read.
Total Errors Total number of errors that occurred during a
test run. For the pass/fail criteria, see below.
Field Description
Run
Start Starts the PosID test procedure.
Stop Stops a running test procedure; the current
Actual Read is completed before
interrupting the test.
Close Finishes the function.
Reference Read
Field Description
Save Saves the Reference Read data in the
PId2000.ini file in the C:\Program
Files\Tecan\Genesis Instrument
Software\Service directory.
Load Loads the Reference Read data from the
PId2000.ini file. No new Reference Reads
are done.
Show Carrier Option to select the carrier reads data view.
Reads Only the carrier read data for all defined
worktable positions are displayed in one
table.
Show Option to select the carrier read and
Container container read data view (refer to Figure
Reads 11.11.35 on page 11-95).
Completion of Test
When the test is completed, the message PosID 2 Test passed
or PosID 2 Test failed displays.
1. Confirm with OK. The list of values in the PosID 2 Test
Results window is complete.
2. After finishing the test, select Close to close the window.
The PosID 2 Test Configuration dialog is active again.
3. Print the test results with Print and/or finish the function
with Close.
Pass/Fail Criteria
The test passed after meeting the following requirements (refer
to Figure 11.11.35 on page 11-95, Total Errors section).
No Tube: 0 errors
No Read: not more than 5 errors
Wrong Read: 0 errors
At least 5 Actual Reads (Number of cycles) must be
performed.
If the test failed, check for correct positioning of the bar code
labels and bar code types (Bar Code Types, page 11-88), and/or
setup of the PosID (Positive Identification Functions for PosID
2, page 11-69).
Advanced Configuration
This function allows FSEs to define or change additional
parameters.
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Controls
The PosID 2 Test - Advanced Configuration dialog includes
the following controls.
Control Description
Carrier List to select a specific carrier to change its
Parameter parameters: Reading Speed and Decode
Safety can be set individually for each
carrier. The Reference Read Speed Factor
remains the same for all carriers.
Reading Field for movement speed, in 1/10 mm/s,
Speed in X- and Y-direction during the read
procedure.
Control Description
Decode Field to set the number of times the
Safety scanner reads the bar codes. This sets the
number of matching decodes required
until a bar code read is accepted as
successful. Range: 1 (low) to 10 (high). The
default setting is 2.
The ratio of Decode Safety and Reading
Speed must be either: high Decode Safety
and low Reading Speed; or low Decode
Safety and high Reading Speed. If not, an
error message displays.
Scan Scanner head-specific parameters; not
Window Min editable.
Scan Scanner head-specific parameters; not
Window editable.
Max
Set default Sets Reading Speed and Decode Safety to
the default values for the selected Carrier
Parameter.
Reference Read Option buttons to reduce the Reading
Speed Factor Speed during the Reference Read to the
selected fraction for a safer read of the bar
codes. For the remaining Actual Reads, the
read is done with the defined Reading
Speed.
Global Checkboxes to eliminate the safety
Optimization movements in B- and Y-directions for a
faster run.
Options
Send bar Checkbox to send the bar code setting
code settings defined with <System Devices><PosID>
on start <Bar code Types> (Bar Code Types, page
11-88) to the PosID at the start of the test
run.
Control Description
Use If this checkbox is selected, the No Tube
NT-Sensor (NT) sensor is switched off during the read
alternate procedure (i.e., when the gripper finger
moves the rack backward). The No Tube
sensor is activated when the gripper finger
returns the rack to its position. This
procedure reduces the risk of read failures,
because the No Tube sensor and the
scanner usually work concurrently.
Set bar Opens the Bar code Settings dialog to set
codes the bar codes (refer to Bar Code Types, page
11-88).
OK Finishes the advanced configuration and
saves the defined values.
Cancel Aborts the advanced configuration without
saving any changes.
Movement
Purpose
The Movement function allows movement of the PosID
according to the entries.
Control Description
Move X List to select the movement step size,
in mm, in the X-direction.
Left Moves the PosID arm from right to left
in the X-direction.
Right Moves the PosID arm from left to right
in the X-direction.
Move Y List to select the movement step size,
in mm, in the Y-direction.
Rear Moves the PosID gripper from front to
rear.
Front Moves the PosID gripper from rear to
front.
Control Description
Move Beam List to select the rotation step size, in
Angle degrees, in the B-direction.
CCW Rotates the scanner head
counterclockwise.
CW Rotates the scanner head clockwise.
LiHa
The m2000sp instrument is equipped with one 8-tip Liquid
Handling arm (LiHa). The LiHa is used for pipetting actions in
different volume ranges. Volumes depend on the tip types
used and features of the liquid system.
Liquid Handling
Arm
Movement Axes
LiHa movements are coordinated with other system devices
such as the RoMa. There are four different movement axes.
Ys
Y Z
Required Tools
Tools required for performing the adjustments and tests
described in this section depend on the installed tips:
• Reference tip—The reference tip is necessary for checking
the Z-range and the reference positions in the X- and
Y-axis.
IMPORTANT: There are now two LiHa reference tip
! types available. Either can be used for S&S functions;
however, procedures for mounting each type differ
significantly. Refer to Reference Tips on page 4–54 of
Section 4.
Before Starting
During some of the following setup and test procedures, the
LiHa moves to different positions on the worktable. Ensure no
objects are installed that could be in the way during these
procedures.
Panel Description
The LiHa panel provides several procedures to be executed.
Generally, the following steps are performed.
1. Start the LiHa panel LiHa Panel, page 11-108.
2. Select the setup/test procedures to be performed and
modify the corresponding settings.
There are two ways to proceed:
• Select ALL Setup and Test checkboxes (聺) at the same
time and enter the necessary data by selecting the
corresponding tabs. Select Start then all setup/test
procedures are performed in one run.
• Select one or more Setup or Test checkboxes and
define the necessary data as described. Select Start to
perform the currently selected setup procedures.
• If the second method is selected, start with the
topmost setup procedure and work downward. Use
the second method to repeat an individual setup.
Before starting any setup procedure, the Operator field
on the Printing page should be completed.
3. Start the desired procedure with Start.
4. After performing any test, refer to Information Page, page
11-133.
5. Print the test results. Refer to Printing, page 11-135.
6. Exit the panel with Close.
Refer to
Operator FSE
Section
Set Default Parameters X Set Default
Parameters,
page 11-118
Determine Range X Determine
Range, page
11-118
Calibrate Lower DiTi Eject X Calibrate the
Lower DiTi
Eject
Protocol,
page 11-125
Determine Reference X Determine
Positions Reference
Positions,
page 11-121
Determine Spacinga Determine
Spacing, page
11-123
Individual Zb X X Individual Z
Alignment,
page 11-124
Arm Position Accuracya
Tip Adapter X X
Information X X Information
Page, page
11-133
Printing X X Printing,
page 11-135
Test Report X X Test Report,
page 11-136
a. This function is for production only and is not accessible to FSEs.
b) Not used with the m2000sp.
Directory, Files
The LiHa function can create the following files. Refer to
Liquid Handling Arm (LiHa), page 11-103 for details.
• Result Files
Description
Saved in C:\Program Files\Tecan\Genesis Instrument
directory: Software\Results
File name: LiHa_<serial_number>_<date>_<time>.any
LiHa Panel
Pages
The LiHa panel is subdivided into the following pages.
Page Description
Contents General overview, procedure/test selection.
Channel Setup/check of tips (length/types) and
syringe sizes.
Setup Definition of tips and other parameters for
the test.
Test For Tip Adapter and Arm Position Accuracy
Configuration tests; Arm Accuracy test is for production
only.
Page Description
Information Device parameters and diagnostic data; refer
to Information Page, page 11-133.
Printing Print selection for the Test report; refer to
Printing, page 11-135.
Move LiHa Movement tool.
Dilutor Tool used to check dilutors and tubings.
Controls
The Contents page includes the following controls.
Control Description
Available Devices Shows the connected LiHa.
Set Default For setting default values.
Parameters
Determine Range For determining Y and Z range; refer
to Determine Range, page 11-118.
Determine Reference For determining/checking reference
Positions positions; Determine Reference
Positions, page 11-121.
Determine Spacing For calculating spacing. Not used on
the m2000sp.
Arm Position Production function, not accessible
Accuracy to operators and FSEs.
Tip Adapter Tip adapter test.
Individual Z For determining/checking Z-range
of each individual tip. Not used on
the m2000sp.
384 Well Tip Adjust Not used on the m2000sp.
384 Well Tip Verify Not used on the m2000sp.
Channel Page
Purpose
The Channel page allows the operator to check and define the
following:
• Tip configuration
• Lengths of user-defined tips (FSE password required)
• Syringe size (FSE password required)
Controls
The Channel page includes the following controls.
Control Description
Ch Channel number.
Type Type of the installed tip.
Length Length difference, in mm, of the tip relative
to the reference tip. A positive value
indicates the tip is longer than the reference
tip, a negative value that it is shorter.
DiTi Can be checked if the respective tip is a
DiTi.
Pinch Checkboxes for selecting LowVol tips. Not
used with the m2000sp instrument.
Active Indicates the tip is an active tip; the
checkbox cannot be set and the system
automatically determines if the tip is active.
Not used with the m2000sp instrument.
Syringe Syringe volume in µL (microliter).
Group Checkboxes to set identical parameters for
multiple channels. In this case, channels
1 to 8 have identical parameters. Set the
parameters for channel 1 and select the
Group boxes. Parameters of subsequent
channels (if their Group boxes are not
selected) are set to equal those of channel 1.
The tip length can be set on the Channel page. Tip lengths do
not require edits if the tips are supplied by Tecan.
Perform the following steps to set the tip length.
1. If not already done, start the LiHa panel and select the
Channel page.
2. Check the length of the tips in use and enter the difference
between the reference tip in the appropriate field.
3. Confirm the entries:
• Select OK to store the configuration then exit the
LiHa panel.
• Select Apply to save the entries then perform further
setup/test procedures within the LiHa panel.
1. If not already done, start the LiHa panel and select the
Channel page.
2. Check the volumes of syringes installed on the
instrument. To set the correct values, use the up or down
arrows of the corresponding spin boxes.
3. Confirm the entries:
• Select OK to store the configuration then leave the
LiHa panel.
• Select Apply to save the entries then perform further
setup/test procedures within the LiHa panel.
Purpose
The Move LiHa page allows movement of the whole LiHa or
parts of it in the appropriate axes: X, Y, XS, Z1 to Z8. Refer to
Move LiHa Page, page 11-114 and LiHa, page 11-104for details.
Controls
The Move LiHa page includes the following controls.
Control Description
All Tips/Tip1/ Checkboxes to select the tips to be moved in
Tip8 the Z-direction.
Control Description
Single step, in 1/10 mm, movement
buttons.
Ten steps, in 1 mm, movement buttons.
Keyboard Control
The keyboard can also be used for movements in the various
axes. Refer to View and Tools Functions, page 11-34 for details.
Setup Page
Purpose
The Setup page provides controls for setting the parameters for
the various setup procedures.
Controls
The Setup page includes the following controls.
Control Description
Determine Frame with controls to specify the
Reference conditions for determining the reference
Positions positions.
automatically Option button for automatic determination.
manually Option button for manual determination.
Verify with Checkbox selected if the “normal” tip is
mounted tip used for manual verification instead of a
reference tip. Not used with the m2000sp
instrument.
Reference Frame to set the reference positions.
Positions
Grid Spin boxes to set the left and right reference
grid positions.
Use for Y Option buttons that specify if the reference
positions in the Y-axis should be determined
at the left or right reference position.
4/8 Reference Checkbox selected if a references tip is
Tips Available mounted at each tip adapter.
Use Y-backlash Checkbox must be selected for the m2000sp
instrument.
Input box cannot be set by FSE.
ABD arm For production only, cannot be set by FSE.
Not used with the m2000sp instrument.
Initialize Position Spin box used if a Te-MO is installed on the
X instrument. Defines the position relative to
grid 1 at which the arm is initialized. The
position must be chosen to eliminate
possible collisions with the Te-MO. The
position can be set with an accuracy of
0.1 [mm]. Not used with the m2000sp.
Table below Shows current speed and acceleration in X-,
Reference Y-, and Z-axes.
Positions
Purpose
This function resets drive properties to the default values. It
should only be used:
• When a new instrument is set up the first time.
• After certain repairs from which maladjustment could
result, e.g., removal and reinstallation of the worktable,
replacement of entire LiHa, etc.
Procedure
Perform the following steps to set the default parameters.
1. On the Contents page, select the Set Default Parameters
checkbox (聺) then select Start.
2. When finished, all ranges, reference positions and
spacings must be adjusted with the respective functions.
Determine Range
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Determine Range procedure performs the following:
• Moves all tips back and forth in the Y-direction to
determine the available Y-range.
• Moves the tips one by one in the Z-direction to determine
available Z-ranges. A reference tip must be mounted at the
tip position whose Z-range is being determined.
• Determined values are written to the LiHa.
Procedure
Depending on the quantity of reference tips available, the
following instructions can vary slightly.
• If there is only one reference tip available, the software
prompts when to mount it to a particular tip position.
• If there are enough reference tips available for all tip
positions, a reference tip can be mounted to each position
before starting the test.
7. Starting with the first position, one tip adapter after the
other is moved downward until the reference tip mounted
to it touches the worktable surface. After the Z-range is
determined, the respective tip is moved up again.
• If there is only one reference tip available, the
software prompts when to attach it to the next
position. Confirm with Done. The program continues
with the next position.
• If there is a reference tip at each position, the software
automatically continues with the next position.
8. After the Z-ranges of all tip positions are determined, the
operator is prompted to remove the reference tip(s).
Purpose
The Determine Reference Positions procedure moves the
LiHa with the driven tips to the selected reference positions in
sequence.
• To adjust tips and to calibrate the X- and Y-axes.
• To write the results to the LiHa.
NOTE: The “driven” tips are tips 1 and 3 on 4-tip
instruments, tips 3 and 7 on 8-tip instruments.
Reference Positions
Reference Tip
Reference Point
Procedure
Perform the following steps to determine the reference
positions.
1. On the Contents page select the Determine Reference
Positions checkbox (聺) and switch to the Setup page
(Figure 11.11.40 on page 11-119).
Determine Spacing
Based on results of the previous procedure, spacing is
calculated automatically. No further adjustments are required.
The results are written to the LiHa.
Individual Z Alignment
Purpose
This alignment is not used when running m2000sp software.
Additional alignments on the worktable are performed using
m2000sp software. The purpose of the Individual Z is to set
the bottom ends of non-active tips to the same height.
Purpose
The Check Tip Adapter function checks the electrical contact
for the DiTi and Liquid Detection functions.
Procedure
Perform the following steps to check the tip adapters.
1. On the Contents page, select the Tip Adapter checkbox
(聺).
2. Remove all DiTis from the DiTi adapters.
3. Switch to the Test Configuration page (refer to Figure
11.42).
3. Select Start.
Dilutor Tool
Description
The Dilutor tool checks the dilutors and associated tubings.
Each dilutor can be activated individually. For example, this
tool allows the FSE to determine the following:
• If the dilutor address switches are set correctly. For
example, if the same address is assigned to two different
dilutors, one of them cannot be activated.
• If each dilutor is connected (via the tubing) to the correct
LiHa channel. For example, if liquid coming from
dilutor #1 is dispensed through tip #3, it is connected to
the wrong channel.
Controls
The Dilutor tool includes the following controls.
Control Description
Sliders 1 to 8 There is one slider per channel. The sliders
aspirate and dispense liquid through the
associated dilutors.
Init buttons 1 to For initializing dilutors individually.
8
Text fields 1 to 8 Displays the volume, in µL, aspirated or
Volume to dispensed by the respective dilutor. If the
aspirate/dispen indicated value is positive, the indicated
se [µL] liquid quantity is aspirated; if negative, it
is dispensed.
Flush tips once Initializes the dilutors one by one. The
sequentially syringe volume is aspirated then dispensed
through the respective tip.
Flush Flushes or empties the liquid system.
Wash station Location of the wash station.
Grid:
Position: Position of the wash station.
Use Fast Wash Select to use the FaWa pump to flush.
Pump:
Fill Duration Duration of the filling.
Flush Initialize the flush protocol.
Error Handling
Errors occur:
1. If the wrong dilutor reacts, check the address settings.
Note the address number corresponds to:
• Dilutor # minus 1 (i.e., dilutor 1 has address 0, dilutor
8 has address 7).
2. If liquid emerges from the wrong tip, check the
connection of tubing between the dilutors and tips.
3. Check the tubing for leaks, and to verify they are not
kinked or damaged. Replace damaged parts.
Information Page
Purpose
The Information page summarizes device parameters and
diagnostics data.
Data
The Information page includes the following controls.
Control Description
Refresh Reloads the diagnostic data and refreshes
the Sample Time.
Sample Time Snap shot time, e.g., time when the
diagnostic data were read.
Device Parameters
Axes Movement axis: X, Y, Ys, Z1 to Z8.
Offset Offset for each axis, in mm.
Displ Displacement for each axis, in mm.
Range Range for each axis, in mm.
Scale Scale factor.
Accel Acceleration for each axis, in mm per
square sec.
Control Description
Speed High speed of each axis, in mm per sec.
Move Speed Working speed of each axis, in mm per
sec.
Diagnostics Data
Moves Number of overall moves per axis.
Moves Number of clearable moves per axis.
Distance Total moved distance, in meters, per axis.
No-Loads Total number of no-loads, e.g., axis
crashed.
Fetched DiTis Total number of fetched DiTis per tip.
Piercings Total number of piercings per tip.
Status The status of the axis in question (ready,
initialization error, not implemented,
not initialized, not loaded, unknown).
Viewing Data
Perform the following steps to access the Information page.
1. Switch to the Information page (Figure 11.11.47 on page
11-133).
2. Select Refresh.
3. View the data.
4. Print the setup results with Print or preview the Test
report (Printing, page 11-135).
Printing
Purpose
The Printing page allows formatting of a Test report to meet
laboratory requirements.
Controls
The Printing page includes the following controls.
Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Device Checkbox to select device data.
Configuration Checkbox to select configuration data
as shown on the Information page
(Figure 11.47).
Parameters Checkbox to select device parameters
as shown on the Information page
(Figure 11.47).
Diagnostics Checkbox to select diagnostic data as
shown on the Information page
(Figure 11.47).
Setup Results For printing the setup results.
Control Description
Tip Adapter Checkboxes to select tip adapter
results.
Detailed
Results
Arm Position Not relevant (manufacturer
Accuracy procedure).
Multiple existing Data selection valid for all device data
Sections selected in the Detailed Print Section
Configuration section.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to
setup a printer.
Printing a Report
Perform the following steps to print a report.
1. Switch to the Printing page (Figure 11.48).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the Test report onscreen with Result Preview.
4. Select Print to print the Test report.
Test Report
Depending on selections, the Test report includes the
following data.
Description
Header Global data such as software versions,
LiHa and instrument serial numbers, date
of creation, and global test review (not
relevant).
Signature section with operator name.
Description
Device
Configuration Address, firmware and bootware version,
number of tips, spacing.
Device Axes, offset, displacement, range, scale,
Parameters acceleration, speed, move speed.
Diagnostics Same values as on the Diagnostics page:
Axes, moves, distance, no-loads, fetched
DiTis, piercings, status.
Setup Results
Deviation: Shows deviations of the arm for
movements in xa and y directionb.
Arm Position Not done (manufacturer procedure).
Accuracy:
Tip Adapter Detailed results, test configuration,
configuration details.
a. Deviation in X-axis: Note that two values are shown, one
per driven tip (tips 1 and 3 on 4-tip instruments, tips 3 and
7 on 8-tip instruments).
- If the X-deviation is 0, the arm moves exactly at a right
angle (90°) to the front edge of the worktable.
b. Deviation in Y-axis is 0 if the arm moves exactly parallel to
the worktable in the X-axis.
Move Test
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instruments safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
Purpose
The Move Test function verifies correct functioning of the
drives of the LiHa, RoMa, PosID, and Dilutors. For other
options, a range test (Range Test, page 11-140) and/or a
Random Move Test (Random Move Test, page 11-141,
corresponds to the burn-in or validation procedure) can be
performed.
NOTE: Before starting the Move Test function, ensure
the worktable is empty and free of obstacles at all times
since the drives move with high speed.
Panel Description
The Move Test function provides two procedures to be
executed. Generally, the following steps must be performed:
1. Start the Move Test panel Move Test Panel, page 11-139.
2. Select the test to be performed and modify the
corresponding settings, if necessary.
NOTE: Before starting any test procedure, the Operator
field on the Printing page should be completed.
3. Start the desired procedure with Start.
4. After performing any test, check the test results Results,
page 11-143.
5. Print the test results Printing, page 11-146.
6. Finish with Close.
Files, Directory
The Move Test function creates the following files. Refer to
Section 4, Installation for additional information.
Description
Result files Directory C:\Program
Files\Tecan\Genesis
Instrument Software\Results
Name MoveTest_<serial_number>_<
date>_<time>.any
Configuration Directory C:\Program
files Files\Tecan\Genesis
Instrument Software
Name <name>.anya
a. Enter the full file name (with extension.any)
Pages
The Move Test window is subdivided into the following pages.
Page Description
Contents Test selection.
Test Configuration of the random move test.
Configuration
Results Test results summary; refer to Results, page
11-143.
Printing Print selection for the Test report; refer to
Printing, page 11-146.
Controls
The Contents page includes the following controls.
Control Description
Range Performs a Range test; refer to Range Test,
page 11-140.
Random Performs a Random Move test; refer to
Random Move Test, page 11-141.
Range Test
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Range Test procedure determines if the installed LiHa,
RoMa, and PosID can reach the whole range. Each device
moves along its axes to its extreme positions. The evaluated
range is compared to the stored ranges. The tests pass if the
difference between the determined and stored values are
within the deviation limits. The test lasts approximately 5 to
10 minutes, depending on the installed devices.
Procedure
Perform the following steps for the Range test.
1. Select the Range checkbox (聺). No other parameters must
be defined.
2. Start the procedure with Start. The message Ranges Move
Test progress displays.
3. At the end of the test, a message Range Move Test: Passed
or Range Move Test: Failed displays.
4. Confirm with OK.
Pass/Fail Criteria
The test passed if the evaluated Deviation does not exceed the
specified Deviation Limit, e.g., Range = ok.
If the test fails:
• For the LiHa, perform the Determine Range and Spacing
procedures in this section.
• For the RoMa, perform the Determine Range procedure in
this section.
• For the PosID, perform the Autorange procedure in Setup
Procedure, page 11-76.
Purpose
The Random Move Test determines if the mechanics of the
LiHa, RoMa, and PosID work properly. All devices are moved at
random for the defined number of cycles; the default setting is
7500 and lasts approximately 8 to 16 hours. After a defined
number of moves (default setting is 200), an initialization is
performed and the number of lost steps is evaluated for each
axis.
Procedure
Perform the following steps for the Random Move test.
1. Select the Random checkbox (聺) and switch to the Test
Configuration page (Figure 11.50).
2. Enter the number of cycles to be performed in the
Duration field. The default setting is 7500, which lasts
approximately 8 to 16 hours.
Pass/Fail Criteria
Results
Purpose
The current test results are summarized on the Results page.
Using the Test Results option buttons, results for the
corresponding test can be displayed.
Perform the following steps to view the results.
1. Switch to the Results page (Figure 11.51).
2. Use the option buttons in the Test Results frame to select
results to view. The Range option button is selected by
default.
3. Print the setup results with Print or preview the Test
report (Printing, page 11-146).
Figure 11.51: Move Test Panel - Results Page for Range Test
Description
Device Name and address of the tested device.
Axis Tested axis.
Range Result of Range Move test. Tip mounted
indicates the corresponding Z-axis could not be
tested for the whole range, because a tip was
mounted.
Deviation Difference between the distance evaluated
during initialization and the expected distance.
Deviation Allowed difference between the evaluated and
Limit expected distance.
Within Compare result for Deviation less than
Limit Deviation Limit.
Printing
Purpose
The Printing page allows formatting of a Test report to meet
laboratory requirements.
Controls
The Printing page includes the following controls.
Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Range Move Test Checkboxes to select the tests to print.
Random Move Test Each test contains a substructure (expand
with [+]) with the same data selection
properties as the Multiple existing
Sections section.
Multiple existing Data selection valid for all tests selected in
Sections the Detailed Print Section Configuration
section.
Detailed Results Checkbox to print detailed test results.
several devices or Checkbox to print the test results for a
axis specific device or axis.
Operator Field for operator name. An entry is recommended.
Comment Field for additional notes. An entry is recommended.
Result Opens a Test report preview window.
Preview
Printer Setup Starts the standard Windows dialog to setup a printer.
Printing a Report
Perform the following steps to print a Test report.
1. Switch to the Printing page (Figure 11.53).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the Test report onscreen with Result Preview.
4. Print the Test report with Print.
Test Report
Depending on selections, the Test report includes the
following data.
Test Report
Header
Global data, e.g., software versions, instrument serial number, date of
creation, and global test review such as:
Test Result: Passed, if all tests passed; if not, Failed.
Tests done: All or Not all
Test Configuration:Default or User Defined/User Defined [<file
name>], if there were deviations to the default values.
Device Default Settings: not relevant.
Signature section with operator name.
Range Move Test: Passed or Failed or Not Done
Detailed Results Same data displayed on the Results page,
(Results of Range Test, page 11-144)
subdivided into devices.
Random Move Test: Passed or Failed or Not Done
Test Configuration Detailed configuration data.
Details
Detailed Results Same data displayed on the Results page,
(Results of Random Move Test, page 11-145)
subdivided into devices.
Purpose
The Robotic Manipulator arm (RoMa) is used to transport
objects of the format of microplates, such as reagent blocks,
deep well plates, etc. to different positions or for storage onto
the shelf.
The RoMa types, gripper fingers, and rotation
directions—described in the following sections—are important
for correct setup of the RoMa.
RoMa Types
• RoMa with eccentric gripper fingers
Movement Axes
The RoMa’s movements are coordinated with other system
devices such as the LiHa. There are five different movement
axes.
Axis Description
X-axis Movement of the RoMa left-right.
Y-axis Movement of the RoMa forward-backward.
Z-axis Movement of the RoMa gripper head
up-down.
G-axis Movement of the RoMa gripper fingers
open-close.
R-axis Rotational motion of the RoMa gripper
head.
Rotation Directions
Possible rotation directions depend on the RoMa type:
• The RoMa Long can be set mechanically to rotate two
directions:
CW (clockwise) within a range 0° to 270°, start position: 0°
(Figure 11.56, right side). Used for the m2000sp instrument.
90° 270°
Y Z
G
X
Gripper Types
Different types of gripper fingers can be mounted on the
rotator of a RoMa.
• Eccentric gripper fingers, Figure 11.58, can be used for all
types of RoMa.
Required Tools
The RoMa Reference tool and the RoMa Test Plate (Figure
11.60) are required for RoMa function.
Reference Tool
Test Plate
Panel Description
The RoMa panel provides several procedures to be executed.
Generally, the following steps must be performed:
1. Start the RoMa panel Move Test Panel, page 11-139‘’.
2. Select the setup procedures to be performed (refer to Table
61) and modify the corresponding settings.
There are two ways to proceed:
• Select ALL Setup checkboxes (聺) at the same time and
enter the necessary data by clicking the corresponding
tabs. Press Start and all setup procedures are
performed in one run.
• Select one or more Setup checkboxes (聺) and define
the necessary data as described. When Start is pressed,
the currently selected setups are performed.
• If the second method is selected, start with the
topmost setup procedure and work downward. Use
the second method to repeat an individual setup.
• Before starting any setup procedure, the Operator
field on the Printing page should be completed.
3. Start the desired procedure with Start.
4. After performing any setup, check setup results Results,
page 11-143.
5. Print setup results Printing, page 11-146.
6. Finish with Close.
Files, Directories
The RoMa function creates the following files.
Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Results
File name: RoMa_<serial_number>_<date>_<time>.a
ny
RoMa Panel
Pages
The RoMa window includes the standard command buttons,
and is subdivided into the following tabs.
Tab Description
Contents General overview, setup selection.
Setup RoMa configuration.
Test For configuring the Reference Plate test.
Configuration
Printing Print selection for the Test report (Test
Report, page 11-172).
Move RoMa Movement tool (Move RoMa, page 11-158).
Information Device parameters and diagnostics data
(Information Page, page 11-169).
Controls
The Contents page includes the following controls.
Control Description
Available List of connected RoMas (two RoMas
Devices possible on a Genesis Freedom).
Set Default Sets default values.
Parameters
Determine Determines Y and Z range; refer to
Range Determine/Check Range, page 11-161.
Set Type Sets the RoMa type; refer to Set Type, page
11-163.
Determine Determines or checks reference positions;
Reference refer to Determine Reference Positions, page
Positions 11-164.
Adjust Rotator Adjusts the rotator’s offset and scaling; refer
to Adjust Rotator, page 11-168.
Reference Tests if plates are picked up/placed correctly.
Plate
Move RoMa
This function works only if the RoMa was previously selected
on the Contents page.
Purpose
The Move RoMa page allows movement of the whole RoMa,
or parts of it in the appropriate axes: X, Y, Z, G and R..
Controls
The Move RoMa page includes the following controls.
Control Description
Single step, in 1/10 mm or 1/10 degree,
movement/rotation buttons.
Ten steps, in 1 mm or 1 degree,
movement/rotation buttons.
Continuous movement/rotation buttons
when button is kept pressed.
To logical positions (90°/180°/270°) rotation
buttons.
Free Moves all arms to their extreme positions.
Control Description
Grip Grips the test plate.
X, Y Text boxes indicating the current position of
the rotation axis with respect to grid
position #1.
Z Current position in Z-axis (distance of
grippers from worktable).
R Current rotational angle of gripper module.
G Current distance between grippers.
Keyboard Control
It is possible to also use the keyboard for movements in the
various axes.
Setup Page
Purpose
The Setup Page provides controls for setting parameters for the
various setup procedures.
Controls
The Setup page includes the following controls.
Control Description
Rotator Type For specifying the rotation direction.
Gripper Type For specifying the gripper type.
Z-range For specifying the Z-range.
Reference Frame for setting the reference positions.
Positions
Grid Spin boxes for setting left and right
reference grid positions.
Use for Y Option buttons to specify if reference
positions in the Y-axis should be
determined at the left or right reference
position.
Ref. Plate Pos. Spin box to set the grid position of the
Grid reference plate.
Initialize Spin box used if a Te-MO is installed on
Position X the instrument. Defines the position
(relative to grid position #1) at which the
arm is initialized. The position must be
chosen to eliminate possible collisions
with the Te-MO. The position can be set
with an accuracy of 0.1 [mm].
Table on right Current speed and accelerations in X-, Y-,
side Z-, R-, and G-axes.
Purpose
This function resets reference positions, ranges, and offsets to
the original (factory-set) values. It should be used only:
• When a new instrument is set up the first time
• After certain repairs from which maladjustment could
result (e.g., removal and reinstallation of the worktable,
replacement of whole RoMa etc.).
Procedure
Perform the following steps to set the default parameters.
1. On the Contents page select the Set Default Parameters
checkbox (聺) and select Start.
2. When finished, all ranges, reference positions, and
rotation ranges with the respective functions must be
adjusted.
Determine/Check Range
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Determine Range procedure moves the RoMa in Y-, Z-, G-,
and R-direction to its extreme positions to find the available
ranges. The determined values are then written to the RoMa.
To mount the reference tool, the gripper fingers must be
removed from the rotator. Refer to Table 61.
Procedure
Perform the following steps to determine the range.
1. On the Contents page, select the Determine Range
checkbox (聺) and switch to the Setup page.
Set Type
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Set Type procedure allows definition of the following:
• Rotation direction of gripper head
• Gripper finger types
• RoMa type to set up (RoMa Standard or RoMa Long)
Procedure
Perform the following steps to set the RoMa type.
NOTE: Refer to Orientation RoMa Standard on page
3–12 of Section 3, Technical Data to verify screw
position for CW rotation.
1. Select the Set Type checkbox (聺) and switch to the Setup
page (Figure 11.50).
Description
Rotator Type Configures the rotator type.
CW Select this option button so the gripper
head rotates clockwise. The m2000sp
instrument must be set to CW.
CCW Select this option button so the gripper
head rotates counterclockwise. Not used
with the m2000sp instrument.
Gripper Type Configures the gripper type.
Eccentric Select this option button to define
installation of the eccentric gripper
fingers. The m2000sp instrument uses
eccentric gripper fingers.
Centric Select this option button so the centric
gripper fingers are installed. Not used
with the m2000sp instrument.
Z Range Configures the Z-range of the gripper
arm.
Normal Option button to define the RoMa in
(standard) question as a RoMa Freedom (with a
standard Z-drive). The m2000sp
instrument uses a RoMa standard.
Extended Option button to define the RoMa in
(long) question as a RoMa Long (with a long
Z-drive).
Reference Not used in this context; refer to Adjust
Plate Rotator, page 11-168.
Position
Purpose
The Determine Reference Positions procedure moves the
RoMa to the selected reference positions in sequence:
• To adjust and determine the X- and Y- scale factors, and
X- and Y-displacements.
• To check reference positions.
• To mount the Reference tool, the gripper fingers must be
removed from the rotator as illustrated below.
Reference Tool
Reference Pin
Procedure
Perform the following steps to determine reference positions.
1. Select the Determine Reference Positions checkbox (聺)
and switch to the Setup page.
2. Set the Left and Right reference position for the RoMa at
7 and 45, respectively.
3. With the Use for Y option button, select the Left or Right
reference position for movement in Y-direction.
4. Start the procedure with Start. The message Mount
Reference Tool displays.
Adjust Rotator
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Information Page
Purpose
The Information page summarizes device parameters and
diagnostics data.
Controls, Data
The Information page includes the following controls.
Control Description
Refresh Reloads diagnostic data and refreshes the
Sample Time.
Sample Time Snap shot time, e.g., time when diagnostic
data were read.
Device Parameters
Axes Axis for which the test was done.
Offset Offset, in mm, for each axis.
Displ Displacement, in mm, for each axis.
Range Range, in mm, for each axis.
Scale Scale factor.
Accel Acceleration, in mm per square sec., for
each axis.
Speed Normal speed, in mm per sec., for each axis.
Control Description
Move Speed Speed, in mm per sec., at which the RoMa
was actually moved in each axis.
Diagnostics Data
Axis Axis in which the RoMa was moved.
Moves Number of moves per axis.
Moves Number of clearable moves per axis.
Distance Total moved distance, in meters, per axis.
No-Loads Total number of no-loads, e.g., axis crashed.
Status Status of the axis in question (ready,
initialization error, not implemented, not
initialized, not loaded, unknown).
Printing
Purpose
The Printing page allows formatting of a Test report to meet
laboratory requirements.
Controls
The Printing page includes the following controls.
Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Device Checkbox to select device data.
Diagnostics Checkbox to select diagnostic data as
shown on the Information page (Figure
11.51).
Configuratio Checkbox to select the configuration
n data (address, firmware version,
bootware version, Z-range, orientation,
gripper type).
Parameters Checkbox to select device parameters as
shown on the Results page (Figure
11.51).
Control Description
Multiple existing Data selection valid for all device data
Sections selected in the Detailed Print Section
Configuration section.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to
setup a printer.
Printing a Report
Perform the following steps to print a report.
1. Switch to the Printing page (Figure 11.53).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the Test report onscreen with Result Preview.
4. Print the Test report with Print.
Test Report
Based on the selections, the Test report includes the following
data.
Test Report
Header
Global data such as software versions, RoMa and
instrument serial numbers, date of creation, and global test
review (not relevant). Signature section with operator
name.
Device
Diagnostics: Same table as displayed on the Information
page (refer to Figure 11.51).
Configuration: Address, firmware version, bootware
version, Z-range, orientation, gripper type.
Parameters: Same table as on the Results page (refer to
Figure 11.51).
Test Report
Setup Results
Deviation: Shows the deviations of the arm for movements
in X- and Y-directiona
a. Deviations in X- and Y-direction
Y-deviation: is 0 if the arm moves exactly parallel to the
worktable in the X-axis.
X-deviation: is 0 in the Y-axis if the arm moves exactly at a
right angle (90°) to the worktable in the Y-axis.
Command Description
DiTi DiTi (disposable tips) functions; refer to
DiTi, page 11-176.
Liquid Liquid detection functions; refer to DiTi,
Detection page 11-176.
Gravimetric Checks the precision and accuracy of a
Test liquid transfer. Not used with the m2000sp
instrument.
FaWa FaWa functions; refer to DiTi, page 11-176.
6-way valve Checks if the 6-way valve works correctly
and if the valve and its connector are
completely tight. Not used with the
m2000sp instrument.
Licos MPO Licos on MPO functions. Not used with the
m2000sp instrument.
Licos Licos on supervisor PCB functions. Not used
(Supervisor) with the m2000sp instrument.
Balance Sets up balance. Not used with the m2000sp
instrument.
DiTi
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
DiTi Sub-menu
The DiTi sub-menu includes the following commands.
Command Description
DiTi Eject Test Checks the positioning accuracy of the DiTis
and DiTi ejection procedure.
Lower DiTi Tests the put back and disposal ejection
Eject Test heights for the Lower DiTi eject.
Requirements
To execute the Lower DiTi Eject test, the m2000sp worktable
map must be loaded and tips currently in use must be
configured.
Loading a Worktable
Perform the following step to load a worktable.
Load the suitable worktable map with <System><Load
Worktable>.
Purpose
The Lower DiTi Eject Test function tests the putback and
disposal ejection heights for the Lower DiTi Eject. The
calibration function must be performed before the Lower DiTi
Eject test is used.
Procedure
Perform the following steps for the Lower DiTi Eject test.
1. Go to <Liquid System><DiTi><Lower DiTi Eject Test>.
2. Load the m2000sp worktable:
• Place the DiTi Rack carrier at grid 21.
• Place the wash/waste station and DiTi slide in the
cutout at grid 18.
• Place a full rack of 1000 µL DiTis on the rear position
of the carrier.
• Place an empty rack on the center position of the
carrier.
5. Click Select Tips and verify all eight DiTi tips are 1000 µL
DiTi with filter.
6. Select OK.
7. Initiate the test by selecting Run. The status changes to
Test Running.
8. When the test is completed, the status changes to Test
Passed.
Liquid Detection
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
The Liquid Detection sub-menu includes the following.
Command Description
Setup Defines new Liquid Level Detection setups
for user-defined tests.
Normal Test Ensures the Liquid Detection feature works
correctly.
Extended Test This sub-item can only be performed at
manufacturer’s site and is disabled for use by
operators and Field Service Representatives.
Setup
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Setup function defines Liquid Level Detection (LLD)
setups to use with user-defined tests. The LLD setup is not used
by Tecan’s application software such as Gemini and Logic.
Because most liquids used have different capacitive dielectric
properties, the relevant parameters are defined within the
(LLD) Setup. Tests within Setup & Service can use one of
10 pre-defined setups (range 1 to 10) or one of 20 user-defined
setups (range 11 to 30). Each setup defines the LLD procedure,
sensitivity, phase, and In and Out parameters.
Procedure
Load the m2000sp Worktable and perform the following steps
to define LLD setups.
1. Start the function with <Liquid System><Liquid
Detection><Setup>. The Liquid Detection Setups
window displays.
Command Description
Default setups Ten pre-defined LLD setups.
These settings (refer to Table 11-1) cannot be
altered.
User-defined Twenty user-defined LLD setups.
setups
Proc Number of the liquid detection procedure. The
procedure that the liquid detection follows describes
the various steps executed by the LLD.
Sens Detection sensitivity of the liquid detector electronics.
NOTE: Liquids of low conductivity require very
high detection sensitivity (2), and vice versa.
0 Low or standard sensitivity
1 High sensitivity
2 Very high sensitivity
Command Description
Phase This feature is provided because the detection signal of
a tip can interfere with the check function of adjacent
tips at a very high sensitivity level. This especially can
occur if the latter tips enter the same liquid
simultaneously (e.g., in an additive trough rack).
0 0° (in phase). Causes the detector electronics to
check all tips involved in the detection
simultaneously.
1 180° (out of phase). Not used with the m2000sp
instrument.
Causes odd tip numbers to be checked a
fraction of a second later than even tip
numbers.
In Flag to enable the liquid entry signal monitoring
(liquid level detection).
Yes Enabled
No Disabled
Out Flag to enable the liquid exit signal monitoring (clot
detection).
Yes Enabled
No Disabled
Use Trough Checkbox to activate a special liquid detection
Detection Mode mode for containers simultaneously accessed
by several tips.
Close Finishes the function.
Save Saves the LLD setups in the LLDSETUP.ini file in the
<install_path>\Service directory.
Print Opens the Common Print Information dialog to
print the LLD setups.
Double
Proc Description
Detection
Detects with all selected tips
0 no
simultaneously.
Detects with all selected tips
simultaneously. Stores the position of
the first successful detection, retracts
then redetects. The second detection
1 yes
must be within ± 50% of the retract
distance—if not, detection is restarted.
After four unsuccessful detection
attempts an error message displays.
Detects simultaneously with all odd
(1,3,5,7) of the selected tips, and
2 no afterward with all even (2,4,6,8) of the
selected tips.
Otherwise, same as procedure #0.
Detects simultaneously with all odd
(1,3,5,7) of the selected tips, and
3 yes afterward with all even (2,4,6,8) of the
selected tips.
Otherwise, same as procedure #1.
Double
Proc Description
Detection
Tip after tip until all selected tips are
4 no through.
Otherwise, same as procedure #0.
Tip after tip until all selected tips are
5 yes through.
Otherwise, same as procedure #1.
Tip after tip with 80 ms delay in
between, until all selected tips are
6 no through.
Otherwise, same as procedure #0.
Tip #1 moves down, detects, then
7 yes retracts 10 mm. The next tip then
moves down.
Double Detection
no Only one detection.
yes Double detection for increased accuracy
(recommended by Tecan).
Normal Test
Description
Container Form
Tube like Tube is a strip rack or any other rack with
diameter greater than 8 mm.
Well like Microplate well or any small tube with
diameter smaller than 8 mm.
Trough like Reagent bottle or trough accessed by more
than 1 tip.
Conductivity Conductivity of the detected liquid.
Non-conductive liquids, e.g., oil, are not
detected.
NOTE: Liquids of low conductivity
require very high detection sensitivity
(Sens), and vice versa.
low Liquid of low conductivity, e.g., DMSO,
alcohol, deionized water.
medium Liquid of high or medium conductivity, e.g.,
blood, salty water, deionized water.
high Liquid of high conductivity, e.g., blood,
salty water.
all liquids Liquid of high, medium or low
conductivity, e.g., blood, salty water,
deionized water, DMSO, alcohol.
Purpose
The Normal Test function verifies the Liquid Detection feature
is working properly. It is also frequently used to check if Liquid
Detection is able to detect a certain (usually small) volume of a
specific liquid.
In addition, there is an Extended test, which is performed
before the instrument is shipped. It cannot be executed in the
field and is not described here.
NOTE: Tubing should be free of all bubbles. Perform
the Fill/Flush test to remove bubbles.
Procedure
Perform the following steps for the Normal Test function.
1. Load the m2000sp worktable map with <System><Load
Worktable>.
NOTE: If you have loaded the m2000sp worktable, you
must run the test with 200 mL reagent carriers and
troughs.
2. Fill a clear reagent trough with 20 to 30 mL of water. Place
the trough into the first position (RF) of a reagent carrier
and load the carrier on grid 20 of the worktable.
3. Place a full rack of 1000 µL DiTis in the rear position (RR)
of a DiTi carrier and place the carrier on grid 21 of the
worktable.
4. Place a rack with eight clean and dry tubes in positions
1 to 8 on grid 1 of the worktable.
5. Select <Liquid System><Detection Mode> option. Select
Save. Select OK. Select Close.
6. Start with <Liquid System><Liquid Detection><Normal
Test>. The Liquid Detector Test Configuration dialog
displays.
Control Description
Cycles Field for the number of cycles performed.
Enter 15.
Submerge Field for the distance the tip dips into the
liquid. Enter 10.
Is the Liquid Checkbox to define that the liquid source is
Source a Rack? a rack. Select this option.
Source Rack List to select the position of the rack
holding container(s) out of which liquid is
aspirated. Use T1.RF.
Flush Rack List to select the position of the flush carrier
within the wash rack. Select W1.Waste,RM.
Destination List to select the position of the destination
Rack rack. For non-NPS instruments, ensure the
tubes of C4.R1 are empty.
First Dest Field for the destination start position.
Position Position 1 is the upper tube. Enter 1.
Diti Waste List to select the position of the waste
Rack carrier within the DiTi rack. Select
W1.DiTi.Waste.
DiTi Type List to select tip type. Select 1000F EDiTi.
DiTi Tip type.
Diti Rack List to select the position of the DiTi carrier
within the DiTi rack. Select DiTi.RR.
First DiTi Field for the DiTi start position. Position 1
Position is the first one in the upper left. Enter 1.
Run Opens an empty Liquid Detector Test Results
window (Figure 11.74) to start the test
procedure.
Close Finishes the function.
Cancel Aborts the function and resets all values.
Save Starts the standard Windows Save As dialog to
save the data in an appropriate *.ini file in the
<install_path>\Service directory.
Print Opens the Common Print Information dialog
to print configuration data and/or test results.
Control Description
More Opens the Liquid Detector Test—Advanced
Configuration dialog for advanced
configuration (refer to Advanced Configuration
on page 11–192). For FSEs only and requires the
appropriate password.
Select Tips Opens the Select Tips dialog to select the tips
used.
Fill/Flush Opens the Fill/Flush dialog to fill/flush the
liquid dispense system.
Description
Tip-in Results when tip enters liquid.
CurrLevel Current Z-value, in 1/10 mm (measured
from bottom).
MeanLevel Average of all detected liquid levels.
Min-Max Largest difference, in 1/10 mm, between
min. and max. level.
LiqErrs The value is incremented if:
• no liquid is detected when the tip dips
into the liquid (no dip in signal
• no dip out signal is detected double
detection mode)
Level Err Set to yes. If the no dip in signal received
within the required distance, e.g., if the
allowed Liquid Det. Error Limit (Advanced
Configuration) is exceeded.
Tip-out Results when tip leaves liquid.
MeanLevel Average of all detected liquid levels.
ClotErrs Numbered errors, if no dip out signal
received within retract distance, e.g., if the
allowed Clot Det. Error Limit is exceeded. A
dip out signal occurs as the tip leaves the
liquid.
No Exit Numbered errors, if no dip out signal
received.
TotErrs Sum of all errors.
15. After finishing the test, the message Liquid Detector Test
passed or Liquid Detector Test failed displays. Confirm
with OK. The Liquid Detector Test Results window with
the final test results is active.
16. Close the window with Close. The Liquid Detector Test
Configuration dialog is active again.
17. Print the test results with Print and/or finish the test with
Close.
Pass/Fail Criteria
The test passes if TotErrs = 0.
If the test fails:
• Verify all tips are the same height. If not, correct with
<LiHa><Setup><Individual Z>.
• Recheck the liquid system for air bubbles.
Advanced Configuration
Field service representatives can use an advanced test
configuration.
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Procedure
Perform the following steps to enter Advanced Configuration
data.
1. Select More in the Liquid Detector Test Configuration
dialog. The Liquid Detection Test - Advanced
Configuration dialog displays.
Fill/Flush
1. From the Liquid Detector Test Configuration screen
select Fill/Flush.
FaWa
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
The Fast Wash pump (FaWa) is used to pump large amounts of
liquid at high speed through the system, normally to rinse the
tips. The Monitored Pump option (MPO) consists of FaWa and
Licos. The m2000sp instrument only uses the FaWa pump and
the common board for the MPO, and does not use the Licos
system.
Command Description
Setup Configures the Fast Wash pump; refer to Setup,
page 11-196.
Test Checks the throughput of liquid in the entire
Genesis liquid system tubing; refer to Test,
page 11-196.
Setup
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.
Purpose
The Setup function configures the Fast Wash pump (number
of pumps and tips for each pump).
Procedure
Perform the following steps to configure the Fast Wash pump.
1. Start the function with <Liquid System><FaWa><Setup>.
The Fast Wash Pump Setup dialog displays.
Test
Purpose
The Test function checks the throughput of liquid in the
entire m2000sp liquid system tubing.
Test Principle
The following figure illustrates the principle of the liquid level
check with the Fast Wash Pump test.
8-Tip Instruments
Tip type, built-in Duration of Min. throughput
configurations pump… to pass test (µL/s)
(0.1 s)
1000 µL DiTi 30 1600
200 µL DiTi 30 1600
Liquid Level
after second
liquid burst
Test Sequence
The Fast Wash Pump test performs the following procedure to
test the throughput of the pump through the tips or DiTi
adapters.
• Tubes are pre-filled, using Dilutor and without DiTis if the
instrument has DiTi adapters.
• DiTis are picked up.
• DiTi actions only take place if the selected tips are a DiTi
type.
• Level of the pre-fill is measured, using Liquid Detection.
• DiTis are removed.
Procedure
1. Start with <Liquid System><FaWa><Test>. The Fast
Wash Pump Test Configuration dialog displays.
Control Description
Prefill Field for prefill volume in µL. Set the value
Volume so the liquid level reaches the cylindric
part of the destination tube. Required for a
proper liquid level check. The default
setting is 600.
Destination List to select the position of the
Rack destination liquid rack. Select C3.R1 at
grid 4.
Flush Rack List to select the position of the flush
carrier within the wash rack. Select
W1.WASTE,RM.
DiTi List to select the type of DiTi used. Select
Type 1000F EDiTi.
DiTi Rack List to select the position of the DiTi
carrier within the DiTi rack. Select
DiTi.RR. Place the carrier at grid 21.
DiTi Waste List to select the position of the waste
Rack carrier within the DiTi rack. Select
W1.DiTi Waste.
First DiTi Field for the DiTi start position. Position 1
position is the first one in the upper left. Enter 1.
Select Tips Opens the Select Tips dialog to select the
tips used.
More Opens the Fast Wash Pump Test - Adv.
Configuration dialog for user-defined test
configuration.
Fill/Flush Opens the Fill Liquid System dialog.
Run Opens an empty test result window
(Figure 11.79) to start the Fast Wash Pump
test.
Close Finishes the function and saves the values
defined.
Cancel Aborts the function without saving any
changes.
Control Description
Save As Starts the standard Windows Save As
dialog to save the data in an appropriate
*.ini file in the
<install_path>\Service directory.
[Print] Opens the Common Print Information
dialog to print the configuration data
and/or test results. Refer to Printing, page
11-135 for details.
Pass/Fail Criteria
The test passed when the Throughput value is > the minimal
throughput value displayed in brackets in the Tip column. If
Throughput is < the minimal throughput value (in the
example < 1600 µL), the test failed.
The minimal throughput values are user-defined within the
Fast Wash Pump Test - Adv. Configuration dialog (in the
example > 1600 µL).
If Test Fails
1. Verify tips are not blocked.
2. Recheck the liquid system for air bubbles.
Advanced Configuration
Perform the following steps to enter user-defined test data.
1. Select More in the Fast Wash Pump Test Configuration
dialog. The Fast Wash Pump Test - Adv. Configuration
dialog (Figure 11.80) displays.
Control Description
6-way valve Indicates if a 6-way valve is installed. Not used
installed with the m2000sp instrument.
LLD Setup Field for the LLD Setup number; refer to Setup,
page 11-182. The LLD Setup should not be
altered.
User-defined
.. user-defined Checkbox to activate the user-defined test.
test
Name of … Field for a test configuration name, e.g., FaWa.
Duration Field for the duration of the pump sequence,
in 0.1 s.
If changing the duration to a higher value, be
sure to place an appropriate tube with a bigger
volume, e.g., 30, in the destination rack to
avoid an overflow.
Minimal Field to define the minimal expected
Throughput throughput, in µL/s, to pass the test (usually
1600).
Test settings Displays the current test settings.
Select Tip Type Displays the current selected tip, e.g., 1000 µL
DiTi with filter.
Number of Used Specifies the number of tips used.
Tips
Duration Duration of the test.
Minimal Set fill rate to pass test.
throughput to pass
Test
OK Finishes Advanced Configuration and saves the defined
values.
Cancel Aborts Advanced Configuration without saving any
changes.
2. To reduce the number of tips used for the test, click Select
Tips. The Select Tips dialog displays.
Fill/Flush
1. Load the m2000sp Worktable if not loaded.
2. Select <Liquid System><FaWa> Test from the menu.
The Fast Wash Pump Test Configuration screen displays.
12. When the test is running, tap the liquid lines to dislodge
any bubbles lodged in the dispense tubing network.
Options Menu
Menu Items
The following menu displays items that can be included in the
Options menu.
Commands
The Options menu includes the following commands.
Command Description
Easy Option Uninstalls an easy option. Not used with the
m2000sp instrument.
Heated Heated incubator functions. Not used with
Incubator the m2000sp instrument.
Plate Washer Plate washer functions. Not used with the
m2000sp instrument.
Access Status Checks door locks functionality.
Options
Incubation Calibrates and checks the 1 mL Subsystem.
Device
SMIO for NAT Calibrates and checks the system liquid and
waste sensors.
Door Lock V2
Files, Directories
The Access Status Option function creates the following files
(refer also to Setup & Service, page 11-10).
Description
Result Files Directory: <install_path>\Results
File name: AccessStatusOption_<serial
_no>_<date>_<time>.any
Test Directory user-defined
Configuration File name <name>.any (Enter the full file
Files name (with extension .any)
Pages
The Access/Status Panel window includes the standard
command buttons and is subdivided into the following pages.
Page Description
Contents General overview, device, and procedure
selection.
Configuration Test configuration settings and tool buttons.
& Tools
Printing Print selection for the Test report; refer to
Printing, page 11-215.
Controls
The Contents page includes the following controls.
Control Description
Available List of all devices to which Access and Status
Devices options can be connected.
Genesis-CU Front door locks connected to the CU
board.
Access Status Door locks, alarm device V3, and/or
Pause/Resume button connected to the
Access Status Options board.
Setup Setup procedure for supervisor board. Not
used with the m2000sp instrument.
Set Defaults Sets Tecan defaults; refer to EEPROM,
in EEPROM page 11-42.
Control Description
Test Test procedure.
Device Test Tests Access and Status options.
Purpose
The Set Defaults in EEPROM procedure sets internal
configuration data of Access Status options connected to the
SMIO-SAFY board to Tecan defaults. This must be done once if
a new software version is installed.
NOTE: This procedure is only enabled and executable if
the SMIO-SAFY board is selected in the Available
Devices section.
Procedure
Perform the following steps to set the Tecan defaults.
1. On the Contents page, select the following:
• SMIO-SAFY entry in the Available Device section.
• Select the Set Defaults in EEPROM checkbox (聺). No
further parameters need definition.
2. Download the default values to the SMIO-SAFY board and
select Start to have them reread by the Setup & Service
software.
3. Confirm the message Setup: Done with OK.
Procedure
Perform the following procedure to test the front door locks.
1. Select Access Status Options from the Options menu.
6. The prompt Please close the main door for Door Lock 1
(left) displays. Select OK.
9. The prompt Please close the main door for 'Door Lock 2
(right)' displays. Select OK.
Printing
The Printing page allows formatting of a Test report to meet
laboratory requirements. It can be viewed onscreen and
printed.
1. Switch to the Printing page.
Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Device Checkbox to select device data.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to set up
a printer.
Test Report
Depending on the selection, the Test report includes the
following data.
Test Report
Header
Global data such as software versions, device and
instrument serial numbers, date of creation, and global
test review such as:
Test Result: Passed, if all tests passed, otherwise Failed
Tests done: All or Not all
Test Configuration: not relevant
Device Default
Settings: not relevant
Signature section with operator name
Device
Configuration: firmware version and bootware version
Access/Status Test: Passed or Failed or Not Done
Detailed Results
Depending on the tests performed, the following
results are printed:
Door Lock 1 (left): Passed or Failed
Door Lock 2 (right): Passed or Failed
Door Lock 3 (aux): Passed or Failed
Door Lock 4 (aux): Passed or Failed
RT-Incubator 1 (right) Front Door: Passed or Failed
RT-Incubator 1 (right) Slots: Passed or Failed
RT-Incubator 2 (left) Front Door: Passed or Failed
RT-Incubator 2 (left) Slots: Passed or Failed
Alarm Device: Passed or Failed
Alarm Green: Passed or Failed
Alarm Red: Passed or Failed
Pause/Release Button: Passed or Failed
Incubation Device
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
CAUTION: Hot Surface. Identifies an area where the
FSE could be exposed to hot surfaces, such as heater
zones 1 and 2. Allow the area to cool before servicing.
NOTE: This section can only be used with a TEMPO
110 measuring device. Refer to Section 9, Repair,
Replacement of Spare Parts, for an alternate method
for checking heater block temperature.
The Incubation Device panel provides for setup, installation,
and verification of the heating plates that are part of the 1 mL
Subsystem.
Setup
1. From the Main menu, select Options Incubation
Device. The Incubation Device panel displays.
9. Select OK.
NOTE: If the calibration fails, check the
connection to the SMIO board and repeat the test.
NOTE: If the calibration fails, replace the sensor.
14. Replace the sensor only after two (2) consecutive failed
adjustment attempts.
System Bottle
1. Go to the <Options><SMIO for NAT> menu.
2. Select the Sensors Test checkbox (聺).
5. Select Next.
6. The prompt Add 2.0 [liter] of liquid displays.
7. Select Next.
SECTION 12:
SPARE PARTS AND
ACCESSORIES
NOTES
Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-4
Spare Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
System Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
Power Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-6
X-Drive Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-6
Liquid Handling Arm (LiHa) . . . . . . . . . . . . . . . . . . . . . 12-7
RoMa Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-9
PosID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-9
Liquid System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-11
Fast Wash (FaWa) Pump . . . . . . . . . . . . . . . . . . . . . . . 12-11
Dilutors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-11
Access Option and Waste Monitoring . . . . . . . . . . . . 12-12
1 mL Heat-Mag Subsystem (1 mL Subsystem) Components
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-12
Computer, Monitor, and Printer . . . . . . . . . . . . . . . . . 12-13
Cabinet and Instrument Covers . . . . . . . . . . . . . . . . . 12-14
Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-15
Preventive Maintenance Kit . . . . . . . . . . . . . . . . . . . . 12-15
Accessories and Commodities . . . . . . . . . . . . . . . . . . . 12-16
Output Deck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-16
Upgrade Kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Abbottlink and MxN . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Hood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Worktable
Part Description Number
WORKTABLE, ASPS Stainless steel; worktable cutout at 619730
grid 18
PIN SET GENESIS Includes 25 guide pins, 20 stop pins, 04J97-36
5 reference guide pins, and 5 carrier
lock reference pins
PIN LOCK FROG 100 PCE Set of 100 619017
GENESIS/MINI PREP
COVER SCREW WHI. 20 PCE, Screw covers for the worktable; set of 619103
WORKTABLE GENESIS 20
STRIP 200CM SEAL WASTE CHANNEL Rubber sealing strip for the worktable; 619003
WORKTABLE 200 cm
WASH STATION Includes tube connectors 04J72-28
Metal Guide Pins Set 50-148040
PIN LOCK FROG SET 16 PCE 10619007
PIN STOP 100 PCE. GENESIS 10619030
WORKTABLE
PIN GUIDE SET 25 PCE. WORKTABLE 10619439
System Electronics
Part Description Number
PCB OPTIBO FREEDOM 619856
FUSE 6.3 AT SET 10 PCE 6.3 AT, set of 10 619821
FUSE 8A 10 PCE 8 AT, set of 10 619823
PCB CU GENESIS 619828 or
10619301
CABLE PC-OPTIBO 619232
Power Module
Part Description Number
POWER SUPPLY 2 960W RWS/FREEDOM 100-240 VAC, 50-60 HZ, 960 W power 619844
150/200 supply, complete with bracket, lamp
and switch
POWER CABLES, SWISS 04J97-16
POWER CABLES, EUROPE 04J97-18
POWER CABLES, USA 04J97-20
POWER CABLES, UK 04J97-22
X-Drive Assembly
Part Description Number
X-DRIVE COMPLETE GENESIS 619854
FREEDOM
X-BELT 16/250 GENESIS FREEDOM 619855
RoMa Standard
Part Description Number
Roma Grippers RoMa fingers, screws, and 3M tape 50-148125
PCB GRIPPER ROMA Gripper board 619300
MOTOR ROTARY DC SERVO ROMA Rotator motor 619345
OPTION
CABLE FLEX 10 PIN LONG ROMA For gripper interface; 1.61 m, 10 pins 619877
GENESIS FDM
PCB DC SERVO 1 For LiHa; RoMa Z, Y; and PosID Y/B 619303
PCB ROMA GENESIS FREEDOM 619868
Y-BELT ROMA OPTION Y-belt, RoMa 619513
MOTOR COMPLETE Y-AXIS ROMA Motor for LiHa Z-axis and PosID 2 30019825
Y-axis
Z-MOTOR ROMA OPTION Z-motor 619346
BRAKE SOLENOID Z-AXIS COMPLETE Active brake 30027047
ROMA-3
SET ROMA Z BRAKE M2000EVO Brake shoe and spring only 30031034
CABLE FLEX LIHA/ROMA 13 PIN Flex cable for LiHa/RoMa; 13 pins 619866
FREEDOM 150
PCBA DEVICE CU BOARD 1 619050
ARM ROMA SPARE GENESIS FREEDOM RoMa arm, excluding gripper finger 10619888
and flex cable
PCB BACKPLANE ROMA 2 OPTION 619343
PCB DC SERVO POWER 1 GENESIS 619829 or
10626201
Cable Flex Y-axis Back-back Roma FDM. Flex cable Y-axis backplane to RoMa 619339
Freedom backplane
GRIPPER MP OBJECTS OPTION ROMA 612601
ARM
PosID
Part Description Number
Option PosID2 Genesis Entire assembly with pan and X-drive 10612412
X-BELT 5M POSID2 X-belt for PosID 2; 5 m 619611
CABLE FLEX POSID2 GENESIS 100/150 Flex cable 619602
Liquid System
Part Description Number
Y-Distributor 2 Out. MPO Set 5P Genesis 10 mm Y-distributor; set of 5 619410
Tube Aspirating PVC 6.9 MM 5000 MM 619401
Genesis
BLOCK CONNECTOR LIQUID POM 1:4 619406
1/0 M6
TUBE INTERCONNECTING M6 650 MM Tubing to dilutor 619402
GENESIS
Tube Pipetting FEP M6 2.5 1.5 mm 2800 Pipetting tubing 619403
mm
Clamp 9.1 + 9.6 mm 2 SET 10 Pce. 9.1 and 9.6 mm diameter tubing 619414
Genesis clamp sets; 10 each
Dilutors
Part Description Number
DILUTOR XP SMART Includes M6 fittings 10649015
Tools
Part Description Number
ROMA GRIPPERS TAPE 3M Tape for Reference Plate 50-148126
TIP REFERENCE STEEL SHORT LiHa Reference Tip (1 pc) (new style) 30026915
TUBE GREASE 2 PCE. SYS GENESIS Lubrication Grease 10626104
FE500
TOOL CALIBRATING ROMA RoMa Calibration Tool 612622
TOOL ADJUSTMENT ROMA RoMa Teach Plate 613101
RACK SERVICE POSID 613100
HANDLE TRANSPORT SET GENESIS Transport handle set 612003
SCREW SET ASPS Includes 5 each of the following: 619751
M 2x6 mm, M 2x8 mm, M 2.5x6 mm,
M 3x5 mm, M 3x8 mm, M 3x10 mm,
M 4x10 mm, M 4x12 mm,
M 3x12 mm, M 4x8 mm, and
M 4x20 mm
LIHA WRENCH HOLDER 8-69545-01
LIHA Y-SPREAD AID 8-69546-01
REAGENT NEST CALIBRATION TOOL 50-148020
LOCK TRANSPORTATION FREEDOM 30024075
EVO 2 PCE.
PACKAGING BOX GEN.FRDM & FREE- 30024077
DOM EVO
Output Deck
Part Description Number
Peltier Output Deck Assy. Chiller unit D-57217-104
m2000sp Reagent Nest 50-148050
PCR PLATE FOAM INSULATION, 01N98-01
REPLACEMENT
CABLE CHILLER PSU 12V 10619928
PCR Bumpstop 50-148131
Upgrade Kits
Part Description Number
m2000 m1000 Upgrade Kit 09K13-01
m2000sp Worktable Kit 6L60-03
Wheels 8-200918-01
m2000 System Hardware Kit 50-148320
Software
Part Description Number
m2000sp System Software, SCC Series D, 50-148133
Release 3.0
m2000sp System Software, SCC RAD945, 50-148132
Release 3.0
P24 BIOS Upgrade, SCC Series D 50-148140
Hood
Part Description Number
m2000sp Hood Assembly G-Series 02N29-01
Hood Patlite Signal Tower with 50-148340
connector
Hood assembly, gullwing door 50-148341
Hood assembly, C-arm support with 50-148342
window
APPENDIX:
PASSWORDS
Appendix
NOTES
NOTES