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G-series

Service Manual

© 2007 Abbott Laboratories

Abbott Molecular Inc.


1300 E. Touhy Ave.
Des Plaines, IL 60018
200942-103—September 2007
Introduction

NOTES

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200942-103—September 2007
Introduction

Revision Status

Document Revision Section(s) Pages Revised, Software


Control Number Date Revised Added, or Deleted Version

200942-101 11/2005 New Document New Document 1.0


200942-102 12/2006 All Sections All Pages 2.0
200942-103 9/2007 All Sections All Pages 3.0

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200942-103—September 2007
Introduction

Revision Status continued

ISAs Incorporated in this Revision TSBs Incorporated in this Revision

NA NA

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Introduction

Proprietary Statement
The information, documents and related graphics published
herein (the “Information”) are the sole property of Abbott
Molecular. Permission to use the Information is granted,
provided that:
• the copyright notice appears on all copies
• use of the Information if for operation of ABBOTT
products by Abbott trained personnel or informational use
only
• the Information is not modified in any way
• no graphics are used separate from accompanying text
Each person assumes full responsibility and all risks arising
from use of the Information. The Information is presented “AS
IS” and may include technical inaccuracies or typographical
errors. Abbott Molecular reserves the right to make additions,
deletions, or modifications to the Information at any time
without any prior notification.

Qualifications
• All samples (printouts, graphics, displays, screens, etc.) are
for information and illustration purposes only and shall
not be used for clinical or maintenance evaluations. Data
shown in sample printouts and screens do not reflect
actual patient names or test results.
• The Information was developed to be used by Abbott
Molecular-trained personnel, by other persons
knowledgeable or experienced with the operation and
service of the product identified, or under the direct
supervision and with cooperation from Abbott Molecular
technical sales or service representatives.
• In no event shall Abbott Molecular or its affiliates be liable
for any damages or losses incurred in connection with or
arising from the use of the Information by persons not
fully trained by Abbott Molecular. This limitation shall
not apply to those persons knowledgeable or experienced
with the operation and service of the product identified,
or under the direct supervision and with cooperation from
Abbott Molecular technical sales or service
representatives.

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200942-103—September 2007
Introduction

• No part of this media may be reproduced, stored,


retrieved, or transmitted in any form or by any means
without the prior written permission of Abbott Molecular.
• No confidential relationship shall be established in the
event that any user of the Information should make any
oral, written, or electronic response to Abbott Molecular
(such as feedback, questions, comments, suggestions,
ideas, etc.). Such responses and any information
submitted therewith shall be considered non-confidential,
and Abbott shall be free to reproduce, publish, or
otherwise use such information for any purposes
whatsoever including, without limitation, the research,
development, manufacture, service, use, or sale of
products incorporating such information. The sender of
any information to Abbott is fully responsible for its
content, including its truthfulness and accuracy and its
non-infringement of any other person’s proprietary rights.
• Abbott Molecular is not engaged in rendering medical
advice or services.
• Updates to the Information may be provided in either
paper or electronic formats. Always refer to the latest
documents or the most current information.
• Updates to the information in this manual are provided as
ISAs, TSBs, Customer Letters, or Revisions to printed
documents. Always refer to the latest documents for the
most current and up-to-date information.

Warranty Statement
Abbott Laboratories makes no representations or warranties of
any kind or nature with respect to the information. Abbott
Laboratories hereby disclaims all representations and
warranties, whether expressed or implied, created by law,
contract, or otherwise, including without limitation, any
warranties of merchantability, fitness for a particular purpose,
title or non-infringement. In no event shall Abbott
Laboratories be liable for any damages of any kind or nature,
including, without limitation, direct, indirect, special
(including loss of profit) consequential or incidental damages
arising from or in connection with the existence or use of the
Information, regardless of whether Abbott Laboratories has
been advised as to the possibility of such damages.

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Introduction

Trademark Statements
All Abbott Molecular product names and trademarks are
owned or licensed to Abbott Laboratories, its subsidiaries, or
affiliates. No use of any Abbott trademark, trade name, trade
dress, or product name may be made without the prior written
authorization of Abbott Laboratories, except to identify the
product or services of Abbott Laboratories. All other
trademarks, brands, product names, and trade names are the
property of their respective companies. All rights reserved.
Except as permitted above, no license or right, expressed or
implied, is granted to any person under any patent, trademark,
or other proprietary right of Abbott Laboratories.
Copyright 2005–2007 by Abbott Laboratories.
All rights reserved.

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Introduction

Introduction
The m2000sp is an automated system designed to perform
nucleic acid sample preparation through the utilization of
general purpose reagents and an automated liquid handling
platform.

Service Manual
The information was developed to be used by Abbott
Molecular-trained personnel, by other persons knowledgeable
or experienced with the operation and service of the product
identified, or under the direct supervision and with
cooperation from Abbott Laboratories technical sales or service
representatives.

m2000sp Operations Manual


The m2000sp Operations Manual is a complete reference to
the operation of the m2000sp system. The m2000sp
Operations Manual is available for review within the m2000sp
Software under the <Help> menu.

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Introduction

Biosafety Procedures
Use appropriate biosafety practices when handling specimens,
reagents, and contaminated instruments. Refer to Section 2,
Safety for more information.
• Consider all clinical specimens, reagents, controls, and
calibrators that contain human-sourced material and
instrument surfaces or components that have come in
contact with human-sourced material as potentially
infectious. Wear gloves, lab coats, and safety glasses, and
follow other biosafety practices as specified in the OSHA
Bloodborne Pathogens Standard 29 CFR 1910.1030, or
other equivalent biosafety procedures.
• The probes are sharp and potentially contaminated with
infectious materials. Avoid contact with tips (DiTis) of the
probes.
• Dispose of all clinical specimens, reagents, controls,
calibrators, standards (as appropriate), cuvettes, (DiTis)
and other disposables that may be contaminated, in
accordance with local, state, and federal regulations
governing the treatment of regulated medical waste.

Sharps
Sharps, such as contaminated probes, must be placed in an
appropriately marked, puncture-resistant container prior to
treatment and disposal.

Spills
Clean spills of potentially infectious materials and
contaminated instrument components with an appropriate
disinfectant, such as 0.5% sodium hypochlorite, or other
suitable disinfectant.
WARNING: Never use straight household bleach when
cleaning or maintaining the m2000sp. Do not mix any
oxidizing agents, such as sodium hypochlorite in
quantities higher than 0.5%, with the liquid waste of
the m2000sp. Toxic gases could be generated from these
mixtures. Pressure can build up in the closed container
of the mixtures.

Refer to Consumables on page 8–6 of Section 8 for more


information on acceptable cleaning solutions and
disinfectants.

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Introduction

Operators exposed to biohazardous or potentially infectious


materials should take steps immediately to cleanse the affected
area, as follows:
• Eyes—rinse with water for 15 minutes.
• Mouth—rinse with water.
• Skin—wash the affected area with soap and water.
• Puncture wound—allow to bleed freely. Wash the
affected area with soap and water.
• In all cases, seek medical attention immediately.

CAUTION: Personal Injury Risk. Take care to avoid


injury when using sharp tools.
• Do not direct tool points, such as the blade of a
screw driver, toward the face, body, or hands.
• If cutting of tubing, etc., is required, NEVER cut in
the direction of the face, body, or hands.

Chemical Hazards
Operators may be exposed to hazardous chemicals when
handling reagents, calibrators, controls, or bulk solutions.
Exposure to hazardous chemicals is minimized by following
the instructions provided in the assay-specific package inserts
and product-specific Material Safety Data Sheets (MSDS).
Exposure levels are further reduced by the “hands-off” design
features of the instrument when it is used properly.
In general, observe the following precautions when handling
chemicals:
• Consult Material Safety Data Sheets for safe use
instructions and precautions.
• Avoid contact with skin and eyes. If contact with material
is anticipated, wear impervious gloves and protective
eyewear and clothing.
• Always maintain good housekeeping. Do not eat, drink, or
store food and beverages in areas where chemicals are
used.
• If irritation or signs of toxicity occur after exposure, seek
medical attention.

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Table of Contents

Table of Contents

Section 1: About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Section 2: Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Section 3: Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Section 4: Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Section 5: Maintenance and Diagnostic Procedures . . . . . . . . . . . . . . . . . . . . 5-1

Section 6: Upgrade Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

Section 7: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

Section 8: Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

Section 9: Repair, Replacement of Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . 9-1

Section 10: Shutdown, Storage, and Shipping . . . . . . . . . . . . . . . . . . . . . . . . 10-1

Section 11: Genesis Setup and Service Software . . . . . . . . . . . . . . . . . . . . . . 11-1

Section 12: Spare Parts and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-1

Appendix: Passwords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Appendix-1

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Table of Contents

NOTES

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200942-103—September 2007
SECTION 1:
ABOUT THIS MANUAL

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About This Manual Section 1

NOTES

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Section 1 Table of Contents

Table of Contents
TOC

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Purpose of This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Target Groups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Field Service Engineers . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Qualification, Training . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Conventions Used in This Manual . . . . . . . . . . . . . . . . . . . . 1-7
Abbreviations Used in This Manual . . . . . . . . . . . . . . . . . . . 1-8
Reference Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10

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About This Manual Section 1

Introduction

This manual is intended to assist Abbott Field Service


Engineers (FSE) in the installation, service, and support of the
m2000sp G-series instrument.
For users viewing this manual electronically, hyperlinks are
provided from all section Tables of Contents (TOC) and from
appropriate points within certain sections. Users may return to
a previous page by using the Previous View button on the
Adobe PDF bottom navigation bar.

Previous View Next View

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Section 1 Purpose of This Manual

Purpose of This Manual


This manual is a reference guide for experienced FSEs who
have completed service training for the m2000sp instrument,
and are certified to perform the following:
• Site inspection
• Installation
• Initial operation
• Validation
• Site acceptance
• Maintenance
• Repair
• Calibration

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About This Manual Section 1

Target Groups
This manual addresses one target group: FSEs who have access
to most implemented functions.
Some functions are only accessible to Abbott production and
developers.
Functions intended only for FSEs are protected by password.

Field Service Engineers


The terms “Field Service Engineer” and “FSE” always refer to
an Abbott-authorized Field Service Engineer. The FSE is
responsible for initiating operation of the instrument and
performing repairs and periodic maintenance.
Initiating instrument operation includes the first mechanical
installation, installation of instrument software, initial setup,
and startup.
Service includes maintenance procedures or installation of
additional optional hardware modules. If additional hardware
modules are installed, appropriate software must also be
installed. Upon completion, operation of the instrument can
be resumed.
In the event of system problems, contact Abbott Molecular
GSS.

Qualification, Training
An FSE must have attended an m2000sp Service Training
course. Installation, servicing, and repair of an instrument
should only be performed by Abbott-authorized service
personnel.
The FSE must be qualified to operate a PC and be familiar with
basic operation of the Windows operating systems.

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Section 1 Conventions Used in This Manual

Conventions Used in This Manual


The following list provides an overview of the conventions
used in this manual.
• Menus and commands are set in bold and within angle
brackets (< >), e.g., Select <OK> to move on.
• Routes to different functions with the m2000sp software
are presented following the bold/bracket menu
convention and separated by commas, e.g., Start the
function with <System>, <Load Worktable>.
• Directory and file names are printed as follows:
– Path and file names are mentioned directly, e.g., the
default path for the instrument software is
C:\Program files\Abbott\Genesis Instrument
Software\
OR
– A placeholder is given for the directory, e.g.,
<install_path>, which refers to the “real” directory
name
• Placeholders for real names are set in angle brackets < >
e.g., Filename:
TeSonic_<serial_number>_<date>_<time>.any.
• References to this and other manuals are set in italics
and bold text, e.g., Refer to the m2000sp Operations
Manual for more information.

Icons
The following informational symbols are used in this manual:
NOTE: This icon indicates important information or an
exception to conventional methods.
Password Protection: This icon indicates a procedure
that is exclusively for FSEs and requires the use of a
password.
IMPORTANT: This icon is used to indicate information
! that is necessary to the proper functioning of the
m2000sp.
Refer to Notices and Symbols on page 2–5 of Section 2 for a
complete listing and description of safety symbols used in this
manual.

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About This Manual Section 1

Abbreviations Used in This Manual

Abbreviation Description
APC Active Positioning Carrier
CAN Controller Area Network
CAN-BUS Controller Area Network (pathway) over
which data travels
CCW Counterclockwise
CD Compact Disk
CRC Carrier, Rack, and Container, e.g CRC-Editor
CU Control Unit, e.g., Genesis CU, Device CU
CW Clockwise
DiTi Disposable Tip
DLL Dynamic Link Library
DMSO Dimethyl Sulfur Oxide
EEPROM Electrically Erasable Read-only Memory
FaWa Fast Wash Pump
FSE Field Service Engineer
GSS Abbott Molecular Global Service and Support
ILID Integrated Liquid Detection
Licos Liquid Container Supervisor
LiHa Liquid Handling Arm
LLD Liquid Level Detection
M&D Maintenance and Diagnostics
MIO Monitored Incubator Option
MPO Monitored Pump Option
MSDS Material Safety Data Sheet
NAT Nucleic Acid Testing
NATMBS Nucleic Acid Testing Magnetic Bead
Separation
NPS Nano Pipetting System
ODBC Open Database Connectivity
OPTIBO Option Board
PC Personal Computer (also called “SCC”)
PCB Printed Circuit Board

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Section 1 Abbreviations Used in This Manual

Abbreviation Description
PosID Positive Identification
PWR Power
RAM Random Access Memory
RMP Robotic Microplate Processor
ROM Read-only Memory
RoMa Robotic Manipulator Arm
RSP Robotic Sample Processor
RWS Robotic Workstation
S&S Setup and Service
SMIO Smart Monitored Input/Output
SMIO-SAFY Smart Monitored Input/Output Safety
SN Serial Number
SVGA Super Video Graphics Adapter
Te-MagS (Tecan) Magnetic Separation Module
Te-MO (Tecan) Multipipetting Option
TEMPO Temperature Measuring Option
Te-VacS (Tecan) Vacuum Separator Module
TOC Table of Contents Link
UPS Uninterruptible Power Source
UST Ultrasonic Transducer
VCC Volume Control Center
WRC Wash Refill Center

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About This Manual Section 1

Reference Documents

Additional reference documents are listed below but not


enclosed or linked.
• m2000sp Operations Manual Version 3.0, LN 9K20-03
NOTE: The m2000sp Software features an on-line
version of the Operations Manual that is accessible to
all users through the Help menu.

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Section 2: Safety

SECTION 2:
SAFETY

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Safety Section 2

NOTES

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Section 2 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Significance of These Safety Instructions . . . . . . . . . . . . . . . 2-4
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Notices and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
WARNINGS Used in This Manual . . . . . . . . . . . . . . . . . . 2-5
CAUTIONS Used in This Manual . . . . . . . . . . . . . . . . . . 2-5
Warning Notices Attached to the Product or Its
Surroundings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Product Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Use of the Product . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Safety of the Overall System . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
Instrument-related Hazards and Safety Measures . . . . . 2-8
Other Hazards and Safety Measures . . . . . . . . . . . . . . . 2-10
Removal of Safety Elements . . . . . . . . . . . . . . . . . . . . . 2-12
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
When to Decontaminate . . . . . . . . . . . . . . . . . . . . . . . 2-13
Decontamination Method . . . . . . . . . . . . . . . . . . . . . . 2-13
Decontamination Form . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Field Decontamination Checklist . . . . . . . . . . . . . . . . . 2-15
General Safety Rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Legal Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Duty of Maintenance and Care . . . . . . . . . . . . . . . . . . 2-16
Spare Parts to be Used . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16

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Safety Section 2

Purpose of This Section


This section contains specific guidelines for operation and
warnings for hazards with regard to installation, setup,
troubleshooting, maintenance, and repair of the m2000sp.

Significance of These Safety Instructions


Safety of users and personnel can only be ensured if these
safety instructions and safety-related warnings in the other
sections are strictly observed and followed.
Therefore, the m2000sp Service Manual must always be
available to all persons performing the tasks described herein.
In addition to the safety instructions in this Service Manual,
safety instructions in the m2000sp Operations Manual also
apply.

Introduction
This section summarizes the safety instructions applicable to
the m2000sp, and those to be observed when installing,
servicing, or upgrading an instrument. Operation,
maintenance, and servicing of the m2000sp could expose
individuals to potential safety and health hazards. All service
must be performed in accordance with procedures described
herein. This section describes the types and locations of
potential hazards that could cause physical harm to an
operator or service personnel. Warnings and cautions are
provided to alert operators and service personnel of potential
hazards. Standard warning conventions, including hazard
signal words or symbols are described in subsequent sections.

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Section 2 Notices and Symbols

Notices and Symbols


Observe all warning and safety labels applied to the m2000sp.
For an in-depth discussion of these labels, refer to Safety of the
Overall System on page 2–8.

WARNINGS Used in This Manual


WARNING symbols appear as follows:
WARNING: The triangular warning symbol generally
warns of the possibility of personal injury or loss of life
if instructions are not followed. Where possible, the
symbol specifically identifies the hazard.

WARNING: Potential Biohazard.

WARNING: Class 2 Laser Product.

WARNING: Pinch Point.

WARNING: Electrical Shock Hazard.

CAUTIONS Used in This Manual


CAUTION symbols appear as follows:
CAUTION: This indicates the possibility of equipment
damage, malfunctions or incorrect process results, if
instructions are not followed. Other symbols more
specifically indicate the significance of the CAUTION.
CAUTION: Electrostatic Discharge Hazard.

CAUTION: Hot Surface.

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Safety Section 2

Warning Notices Attached to the Product or Its Surroundings


The following table describes significance of the warnings:
Table 2.1: Significance of the Safety Notices

Symbol Significance
Laser Hazard
Identifies a Class 2 laser product that contains an
embedded low power visible Class 2 laser diode bar
code scanner. Warns against direct viewing into the
bar code laser beam or reflections of the beam.
Potentially Infectious
Indicates an area where the FSE or operator could
be exposed to potentially infectious human-sourced
material. Contamination hazards exist if parts of
the instrument are not completely decontaminated.
Wear appropriate personal protective equipment
such as gloves, lab coat, and protective eyewear.
Hot Surface
Indicates an area where the equipment reaches very
high temperatures and caution must be used to
prevent injury.
Pinch Point
Indicates an area where moving parts have the
potential to cause injury if caution is not taken.

Damaged or missing symbols (notices or stickers) must be


replaced immediately. Refer to Section 12, Spare Parts and
Accessories for ordering information.

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Section 2 Product Safety

Product Safety
Use of the Product
The m2000sp instrument is a fully automated system that
performs nucleic acid sample preparation through the use of
general purpose reagents and an automated liquid handling
platform.

Principle
The m2000sp is designed and built in accordance with current
state-of-the-art technology and recognized technical safety
regulations. However, there are risks to users, property, and the
environment if the instrument is used carelessly or improperly.
Warnings in the m2000sp Operations Manual and m2000sp
Service Manual alert the user to these potential dangers.

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Safety Section 2

Safety of the Overall System


In addition to safety instructions included in this manual,
safety instructions of the overall system must also be observed
and followed.
Follow the safety instructions of all instruments used in
conjunction with the m2000sp.

Instrument-related Hazards and Safety Measures


Observe the following safety notices.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

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Section 2 Safety of the Overall System

WARNING: Electrical Shock Hazard. Possible danger


from electrical shock. Switch off the main power and
disconnect the main power connection of the
instrument before replacing fuses, printed circuit
boards, major sub-assemblies, etc. Replace only fuses
that are externally accessible and labeled. Only use
replacement fuses of the specified type and electrical
rating.
WARNING: Class 2 Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing into the bar code laser beam or
reflections of the beam.

CAUTION: Hot Surface. Labels an area where the


operator could be burned from contact with the lamp
or other hot object, such as heater zone 1 and heater
zone 2.

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Safety Section 2

Other Hazards and Safety Measures


Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by following instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.
WARNING: Bleach Restriction. Do not mix any
oxidizing agents such as sodium hypochlorite
(household bleach) with the mLysis, mWash 1,
mMicroparticles, mLysisDNA, mMicroparticlesDNA, and
mWash 1DNA reagents in the Abbott mSample
Preparation System reagents. Do not mix any oxidizing
agents such as sodium hypochlorite (household bleach)
with liquid waste of the m2000sp. Toxic vapors could be
generated from these mixtures, and pressure can build
in the closed container of mixtures. Rinse or clean the
surface initially with water or a weak detergent before
using sodium hypochlorite.

WARNING: Sharp Hazard. The disposable tips (DiTi)


used by the m2000sp have sharp points.
• Take appropriate action when handling DiTis.
• Wear appropriate personal protective equipment,
such as gloves, lab coat, and protective eyewear.

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Section 2 Safety of the Overall System

CAUTION: Personal Injury Risk. Take care to avoid


injury when using sharp tools.
• Do not direct tool points, such as the blade of a
screw driver, toward the face, body, or hands.
• If cutting of tubing, etc., is required, NEVER cut in
the direction of the face, body, or hands.

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200942-103—September 2007
Safety Section 2

Removal of Safety Elements

The protective and safety devices installed on the m2000sp


must not be removed or disabled during instrument operation.
If such components are removed (e.g., for maintenance),
operation should only be resumed when all protective and
safety devices are reinstalled completely and checked.
The protective and safety devices include:
• Safety door and panel
• Left and right service access doors
• Top cover
• Door locks
• Arm guide
• Safety symbols

Figure 2.1: Laser Warning Labels

In addition, some G-series models include a hood.

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200942-103—September 2007
Section 2 Decontamination

Decontamination
When to Decontaminate
In addition to regular decontamination, the user must
thoroughly decontaminate the instrument according to
standard laboratory regulations in the following cases:
• Before any maintenance or service is performed on the
instrument
• Before an Abbott FSE performs on-site service on the
instrument
• Before the instrument is returned to Abbott (e.g., for
repair)
• Prior to storage of the instrument
• Prior to disposal of the instrument or parts of it
• In most cases, before the instrument or individual parts
leave the user’s site

Decontamination Method
The decontamination method must be adapted to the
respective application and associated substances used by the
operator. The owner assumes full responsibility for appropriate
decontamination of the entire instrument.
Refer to the m2000sp Operations Manual for specific
decontamination instructions.
WARNING: Potential Biohazard. Contamination
hazards exist if parts of the instrument are not
completely decontaminated. Components with direct
contact (or the potential for direct or accidental
contact) with chemical or biological material must be
treated, including the tubing system and all upstream
equipment.

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200942-103—September 2007
Safety Section 2

Decontamination Form
Before an Abbott FSE performs any service on the instrument
or before the instrument is returned to Abbott Molecular, the
instrument owner must confirm in writing that
decontamination was performed properly and in accordance
with good laboratory practice guidelines. For this, the owner
must enclose the declaration (Field Decontamination
Checklist), provided below.
Abbott Molecular will refuse any instrument, or part of it, if
the decontamination form is not completed and duly signed.
Refer to the m2000sp Operations Manual for specific
decontamination instructions.

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200942-103—September 2007
Section 2 Field Decontamination Checklist

Abbott Laboratories: Abbott Molecular


Document Type: Form Document Number: AM19-01-003-F02 Revision: 001
Title: Field Decontamination Checklist Approved Date: 27 FEB 2006 Effective Date: 6 MAR 2006

Field Decontamination Checklist


All instruments that have been used to process any potentially infectious materials, which includes Abbott
reagents or controls that contain human serum, must be decontaminated prior to relocation/shipping to minimize
the risk of exposure to personnel involved in the handling or transport of these units.
Please refer to the instrument specific decontamination in the instrument operations manual for more detail.
Complete the following checklist, as applicable, for the instrument and attach it to the instrument so that it is clearly visible
to the Transport Company workers.

Please mark:
I. X for completed
ITEM DESCRIPTION II. N/A if not required by
operations manual
1 Remove all specimens.
2 Remove all reagents.
3 Decontaminate/flush fluid pathways with buffer or other fluid if indicated.
4 Empty and rinse out waste container(s).
5 Remove and drain hoses. Plug drain holes as necessary.
6 Wipe surface of instrument with a detergent solution.
7 Wipe surface of the instrument with an appropriate disinfectant per the
operators manual
8 Affix this Field Decontamination Checklist to the instrument

Completed by:
___________________________ ___________________________ _____________________ ________
Signature Printed Name Title Date

Instrument Serial #: _________________________________

Institution Name: _________________________________

RMA Number: _________________________________

This information is confidential to Abbott. The user is responsible for confirming use of the current Issued Version prior to use.

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200942-103—September 2007
Safety Section 2

General Safety Rules


Legal Regulations
Legal regulations, such as local, state, and federal laws which
prescribe the use or application, and the handling of
dangerous materials in connection with the m2000sp, must be
strictly followed.

Duty of Maintenance and Care


The user is responsible for ensuring the m2000sp is only
operated in proper condition, and that maintenance, service,
and repairs are performed appropriately and on schedule.

Spare Parts to be Used


Use only Abbott-authorized spare parts for maintenance and
repair.

Modifications
Modifications to the m2000sp are permitted only after prior
consultation with and written approval from the
manufacturer. Modifications and upgrades shall be carried out
by an authorized field service engineer only. The manufacturer
declines any claims resulting from unauthorized
modifications.

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Section 3: Technical Data

SECTION 3:
TECHNICAL DATA

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200942-103—September 2007
Technical Data Section 3

NOTES

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200942-103—September 2007
Section 3 Table of Contents

Table of Contents

Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4


System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Technical Data and Requirements . . . . . . . . . . . . . . . . . . . 3-10
Dimensions and Weight . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . 3-13
Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
Address Settings Overview . . . . . . . . . . . . . . . . . . . . . . 3-16
Jumper Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
Error States CU. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
Cable Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-33
SMIO/SMIO-SAFY Connection Diagram . . . . . . . . . . . 3-33
Wiring Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-34
Hood Wiring Diagrams . . . . . . . . . . . . . . . . . . . . . . . . 3-38
Signal Tower . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-43

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200942-103—September 2007
Technical Data Section 3

Purpose of This Section

This section summarizes the technical data of the m2000sp


instrument and contains an overview of the system,
requirements, settings and hardware, and software and
firmware compatibilities.

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200942-103—September 2007
Section 3 System

System
The m2000sp system is a fully automated system for sample
preparation. The system automates nucleic acid sample
preparation through utilization of general purpose reagents
and an automated liquid handling platform. The operator
controls the system with a personal computer (PC) equipped
with service and operating software. There are three distinct
processes involved in amplified nucleic probe technology:
• specimen preparation
• nucleic acid amplification
• automated amplified nucleic acid detection
The m2000sp system performs the first process, specimen
preparation.
The Liquid Handling Arm (LiHa) has eight sampling tips
arranged on one arm. The LiHa moves left and right (X-range),
and is driven by a Servo motor. Each sampling tip is raised or
lowered by its own Servo motor within the arm. Two
additional Servo motors inside the LiHa arm drive the tips
forward and backward and control the Y-spacing of tips. The
LiHa has independent movement in the Z-direction, and also
has equidistant movement in the Y-direction, from 9 to 38
mm maximum (0.31 to 1.5 in) between tips. The resolution is
0.1 mm in all three axes (X, Y, Z).
Disposable filtered 200 µL and 1000 µl pipette tips are used.
Integral detectors (on the LiHa arm) ensure tip attachment.
While using disposable tips, electronics for tip presence check
can control each Z-axis individually, independent of the
presence or absence of a disposable tip.
The liquid system is a central component of the instrument.
Flexible tubing connects the containers, pumps, valves, and
tips. Precision diluters (syringes) ensure accurate aspiration
and dispensing of liquids and air gaps, the latter to separate
various liquids. System liquid is aspirated from the system
liquid container through aspirating tubing, via liquid
distributors to the diluters.

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200942-103—September 2007
Technical Data Section 3

From the diluters, the system is channeled through the


pipetting tubing to the tips. Each tip is connected to an
Integrated Liquid Detection (ILID) module. Each tip can detect
the surface of a conductive liquid by measurement of changes
in capacitance. When a tip touches the liquid surface, the ILID
detects the change in capacitance and triggers a detection
signal. A signal is produced when moving into a liquid and
when coming out, allowing detection of blood clot aspiration.
The software stores the position at which the liquid detection
signal occurred. It calculates, using aspirated volume and the
vial diameter, the point where the liquid detection signal
should be interrupted. A tolerance for cohesion and hysteresis
effects is considered. If the interruption signal occurs past this
tolerance, it is assumed a clot was aspirated.
The wash/waste station allows washing of tip ends with system
liquid, and also for discarding waste. Surplus liquids are
discarded into the waste container via waste tubing. The
system incorporates the use of a Fast Wash (FaWa) membrane
pump for acceleration of liquid distribution through the
system, and improved reduction of carryover.
Precision pumps are used to distribute liquid using 1 mL
syringes. The resolution of the drive is 3000 steps per full
syringe stroke. Plunger and pipetting speed is controlled by the
software.
A grid position defines the minimal width of carriers. On the
worktable, evenly spaced guides ensure proper positioning of
all carriers according to the grid represented in all software.
The guides also enable sliding of carriers/racks in the
Y-direction, necessary for replacement (loading/unloading) of
carriers/racks during operation, and for PosID identification of
tubes, vessels, etc., on carriers.

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200942-103—September 2007
Section 3 System

The PosID module reads both horizontal and vertical bar codes
for positive identification and positioning of carriers, racks,
and containers. With its gripper, it pulls carriers toward the
rear of the instrument for bar code identification on tubes
then pushes the carrier back into position. Commonly
available bar codes can be read. The presence of a check digit
may or may not be required. For tube racks, a tube sensor
detects the presence of a tube.
Table 3.1: Bar Code Specifications

Description Specification
Readable Sample Bar Code 39 (length flexible)
Codes Codabar (length flexible)
Code 128 (length flexible)
2 of 5 Interleaved (length 2-20 even)
Code 93 (length flexible)
UPCA (length flexible)
Bar Code Length Up to 20 characters, depending on
symbology
Bar Code Density (0.127 to 0.381 mm) (5 to 15 mils)
Print Contrast Ratio 70%
PCS
Bar Code Height 욷8 mm
Bar Code Length Max of 64 mm (without quiet zone)
Speed Pos ID version 2 moves in
X-direction along the worktable
with a speed of 40 cm/sec
Wavelength 670 nm
Laser Class Class 2
Quiet Zone 5 mm on either side of bars

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200942-103—September 2007
Technical Data Section 3

The Robotic Manipulator (RoMa) arm uses grippers to


transport objects, up to 400 g maximum. The resolution is
0.1 mm in all four axes [X, Y, Z, G (Gripper)], and 0.1 for
rotation (R).

Table 3.2: RoMa Arm Specifications

Description Specification
Rotation 270
Angle
Gripper 58 to 140 mm
Range
Precision Below ± 0.4 mm in all directions
Force Maximum force of the RoMa Arm in the
Z-direction is approximately 35 N, gripper
force is adjustable to 4 N maximum. Default
gripper force is 1.4 N.

The RoMa arm moves independently and simultaneously with


respect to the LiHa. The RoMa is mounted on the right side of
the instrument, which reduces the accessible work area of the
LiHa on the right side. Peripheral devices, which must be
accessed by the RoMa, should be positioned at the right side of
the instrument, and in front or at the rear of the system when
possible.
The 1 mL Subsystem is a subassembly which performs
magnetic separation and heating steps during the process. The
subsystem is divided into four zones:
• two heating zones (at 30-80±3°C)
• a magnetic zone
• a room temperature zone
Specimens, calibrators, controls, and reagents are pipetted by
the m2000sp into reaction vessels located in the 1 mL
Subsystem carrier, which is moved to the various locations
within the subsystem for addition of reagents, magnetic
capture, washing, and dispensing to the output deck.

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Section 3 System

The Output Deck is the site of the Peltier chiller, assay reagent
packs, master mix tubes, PCR plates and deep well plates used
during instrument operation. The Output Deck is mounted on
the right side of the instrument. The chiller is maintained at a
temperature range between 5°C and 30°C, ± 3°C, when
required by the Application Specification.
In addition, some instruments include hoods that cover the
worktable. However, hoods are not standard equipment on all
G-series instruments.

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200942-103—September 2007
Technical Data Section 3

Technical Data and Requirements


Dimensions and Weight

Instrument
Table 3.3: Instrument Dimensions

Description Specification
Length 142.9 cm/56.3 in
Length (side service door 176.5 cm/69.5 in
open)
Depth 76.8 cm/30.3 in
Depth (front lid open) 124.5 cm/49.0 in
Height (RoMa extended) 92.0 cm/36.2 in
Height (with hood) 168.4 cm/66.3 in
Weight* 189.3 kg/417.3 lb
Weight (with hood)* 206 kg/454 lb

* Weight includes accessories packed inside the cabinet and


instrument.
NOTE: Hoods are not standard equipment on all
G-series instruments.
IMPORTANT: The height with the hood includes the
! signal tower. REMOVE the signal tower before
transporting an assembled instrument though
doorways or an elevator.

Table 3.4: Instrument Clearance

Description Specification
Clearance 1 m (39 in) around the
instrument
10.2 cm (4 in) minimum
for rear clearance

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200942-103—September 2007
Section 3 Technical Data and Requirements

Cabinet
Table 3.5: Cabinet Specifications

Description Specification
Length 142.9 cm/56.3 in
Depth 79.4 cm/31.3 in
Height 79.5 cm/31.3 in
Weight 103.4 kg/228.0 lb

Worktable Dimensions
Table 3.6: Worktable Dimensions

Description Specification
Accessible X-range (X-travel) 1120 mm/44.1 in
Accessible Y-range (Y-travel) 418 mm/16.5 in
Grid Positions on Worktable 45
Cutout at Grid 18 for Wash/waste Station

Z-Ranges
Table 3.7: Z-Ranges

Description Specification
RoMa Standard 259 mm/10.2 in
LiHa 165 mm/6.5 in (with
standard tips)

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200942-103—September 2007
Technical Data Section 3

Orientation RoMa Standard

1 2 Screw Position 3 4

RoMa on the m2000sp must be set


to CW.
Right

Figure 3.1: RoMa Orientation

Weights
Table 3.8: Weights

Description Specification
LiHa 8 kg/18 lb
RoMa Standard and Gripper 6 kg/13 lb
PosID 11 kg/24 lb
FaWa 2 kg/4.4 lb

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200942-103—September 2007
Section 3 Technical Data and Requirements

Electrical Supply
Table 3.9: Electrical Supply

Description Specification
Line Voltage 100-120 VAC/ 220-240
VAC ± 10%
Frequency 50-60 Hz
Power 1200 VA
Equipment and Safety Class I
Circuit Breaker 15 A
Electrical Outlets Required Minimum of 4:
• instrument
• SCC
• monitor
• printer

Uninterruptible Power Supply (UPS)


Abbott recommends connection of the instrument to the main
power via a UPS. See the Pre-Installation Checklist on page
4–14 of Section 4 for recomendations.
Use of a switched UPS is not recommended.
Specifications: 1500 VA/1300 WATTS/15 AMP

System Liquid
System liquid is supplied directly from the system liquid
bottle. Use distilled or deionized water.

Environmental Conditions
The m2000sp instrument is intended only for indoor operation
and storage.
Table 3.10: Environmental Conditions
Description Specification
Operation 15 to 30°C/59 to 86°F
Temperature
Operation Humidity 30% to 80% relative (non-
condensing) at 30°C/86°F or below
Pollution Degree 2 (Non-specific environment
described as a typical laboratory)

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200942-103—September 2007
Technical Data Section 3

Table 3.10: Environmental Conditions


Overvoltage Category II
Operating Altitude Max 2,000 m/6,600 ft
Operating Noise < 85 dBA measured in 1 m distance
Heat Output 4,100 BTU/1200 WA

Storage Conditions
Protect the instrument from dust and debris with a cover.
Recommendation: store the m2000sp in its original packaging.

Computer Requirements
The computer provided by Abbott Molecular meets or exceeds
minimum requirements to operate the instrument.

Site Requirements
Table 3.11: Site Requirements

Description Specification
Loading Dock Present
Load Capacity of At least 411 kg/907 lbs
Elevator
Minimum Size of Refer to Table 3.4: Instrument
Elevator Clearance on page 3–10 and
Table 3.5: Cabinet Specifications
on page 3–11
Door and Entryway Greater than 815 mm/32 in
Openings
Passageway Corners Combined total width of both
passageways around corners must
be greater than 2,794 mm/110 in

Where the instrument is to be located, there must be adequate


space to:
• place instrument and cabinet
• open doors of instrument and cabinet
• move around the instrument for service
• place the computer

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Section 3 Technical Data and Requirements

For more information, refer to Table 3.4: Instrument Clearance


on page 3–10

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200942-103—September 2007
Technical Data Section 3

Settings
Address Settings Overview
Table 3.12: Address Settings

Address
Module Board
Setting
Access SMIO 2
Option SMIO/SAFY 1
LiHa DC Servo 1 (position 1) * 4
DC Servo 2 (position 2) 3
DC Servo 3 (position 3) 2
DC Servo 4 (position 4) 1
DC Servo 5 (position 5) 0
DC Servo power (position 6) 5
RoMa RoMa Freedom Backplane CU 4
Standard RoMa Freedom Backplane PWR 0
RoMa 2 Servo Y/Z 6
RoMa 2 Servo R/G 7
Diluters Diluter 1 0
Diluter 2 1
Diluter 3 2
Diluter 4 3
Diluter 5 4
Diluter 6 5
Diluter 7 6
Diluter 8 7
FaWa MPO 0
PosID DC Servo (CU PosID) 0
Y/B 2
1 mL CC Mags #3 3
Subsystem CC Mags #4 4

* LiHa positions are noted from top to bottom.


NOTE: For repair and replacement details, refer to
Section 9, Repair, Replacement of Spare Parts.

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Section 3 Settings

Figure 3.2: Addresses

m2000sp G-series Service Manual 3-17


200942-103—September 2007
Technical Data Section 3

Jumper Settings

m2000sp not terminated

(m2000sp not terminated)

Figure 3.3: Settings CU

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200942-103—September 2007
Section 3 Settings

(m2000sp terminated)

Figure 3.4: Settings VCC-Dilback

m2000sp G-series Service Manual 3-19


200942-103—September 2007
Technical Data Section 3

Y2 Y1

Z6 (2) Z3 (3)

Z2 (-) Z7 (-)

Z8 (-) Z1 (-)

Z4 (4) Z5 (1)

X1 ---

-----

(m2000sp terminated)

-----

Figure 3.5: Settings LiHa Freedom Backplane

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Section 3 Settings

SW3
Device CU Adress Switch
#4 = C1
#5 = C3

(m2000sp terminated)

Figure 3.6: Settings RoMa Freedom Backplane

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200942-103—September 2007
Technical Data Section 3

(m2000sp not terminated)

Figure 3.7: Settings CU PosID

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200942-103—September 2007
Section 3 Settings

SMIO
J2 CAN-BUS
J9 Jumper (outer pins jumpered)
J18 System liquid sensor
J19 Liquid waste sensor
J21 Solid waste sensor
SW1 Address switch
(m2000sp terminated)

SMIO-SAFY
J2 CAN-BUS to OPTIBO Bd
J3 CAN-BUS to SMIO Bd
J24/J5LLeft door lock
J23/J6RRight door lock
J9 Jumper (inner pins jumpered)
SW1 Address switch
(m2000sp not terminated)

Figure 3.8: Settings SMIO/SMIO-SAFY

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200942-103—September 2007
Technical Data Section 3

(m2000sp not
terminated)
SW1 and SW2 OFF

Figure 3.9: Settings OPTIBO

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200942-103—September 2007
Section 3 Settings

Error States CU
Table 3.13: Error States
Green Red Error
Stays on/off Stays on/off Fatal hardware error
Blinking Off Normal operation
Blinking On Flash not accessible
Blinking Blinking Power fail circuit failure
Blinking fast Blinking fast Firmware download
required

Cable Connections
Table 3.14: Cable Connections: From/To

From To
Connection Cable Connection Board
Power Module (0760510)
24 V 2008664 J2 Power In 1 Optibo
Freedom
24 V 2008664 J14 Power In Optibo
2 Freedom
J1 Line In 100 to 2240
L/N/PE VAC
50/60 Hz

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200942-103—September 2007
Technical Data Section 3

Table 3.14: Cable Connections: From/To

From To
Connection Cable Connection Board
OPTIBO Freedom (2010113)
J4 2008582 J1 S-CAN IN VCC-DILBACK
Diluter/S-CAN 8 (2008555)
J2 Power In 1 2008664 24V Power Module
(0760510)
J14 Power In 2 2008664 24V Power Module
(0760510)
J1 RS232 2008588 RS232 Multiport
J7 CAN 2008583 J7 O-CAN 1 MPO (FaWa)
Option 1 (2009001)
J8 CAN 2010004 J10 O-CAN 1 CU-PosID
Option 2 (2009457)
J9 CAN 2010406 J1 O-CAN 1 C.C. MagS
Option 3 (2009606)
J10 CAN 2010406 J1 O-CAN 1 C.C. MagS
Option 4 (2009606)
J5 Flex 13 2010278 J1 S-CAN LiHa Freedom
S-CAN Backplane
LiHa only (LiHa)
(2010104)
J15 Flex 13 2010156 J1 Option RoMa
O-CAN (Arm CAN Backplane
R1) (2010111)
J18 O-CAN 2010209 J2 O-CAN SMIO-SAFY
RJ45 (2010225)
VCC-DILBACK 8 (2008555)
J1 S-CAN IN 2008582 J4 Optibo
Diluter/S-CA Freedom
N (2010113)
J38 VCC-1401 2010211 J12 ILID Freedom
(DIL 1-4) (NPS/VCC) (2010158)
(Tip 1-4)
J39 2010211 J13 ILID Freedom
VCC-ILID2 (NPS/VCC) (2010158)
(DIL 5-8) (Tip 5-8)

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Section 3 Settings

Table 3.14: Cable Connections: From/To

From To
Connection Cable Connection Board
LiHa Freedom Backplane (2010104)
J4 Y1 Motor 2008617
J5 Y2 Motor 2008617
J6 Z3 Motor 2008615
J7 Z6 Motor 2008615
J8 Z7 Motor 2008615
J9 Z2 Motor 2008615
J10 Z1 Motor 2008615
J11 Z8 Motor 2008615
J12 Z5 Motor 2008615
J13 Z4 Motor 2008615
J3 Lower Diti 2009603
Eject
J16 X Motor 2010184
J1 SYS-CAN 2010278 J5 (S-CAN) Optibo
Freedom
(2010113)
J2 2010186 J9 ILID Freedom
(2010158)
ILID Freedom (2010158)
J9 2010186 J2 LiHa Freedom
Backplane
(2010104)
J22 NPS/J23 2010183 J1 Tip Adapter
Tip Z1 Board
(2010149)

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200942-103—September 2007
Technical Data Section 3

Table 3.14: Cable Connections: From/To

From To
Connection Cable Connection Board
J24 NPS/J25 2010183 J1 Tip Adapter
Tip Z2 Board
(2010149)
J26 NPS/J27 2010183 J1 Tip Adapter
Tip Z3 Board
(2010149)
J28 NPS/J29 2010183 J1 Tip Adapter
Tip Z4 Board
(2010149)
J30 NPS/J31 2010183 J1 Tip Adapter
Tip Z5 Board
(2010149)
J32 NPS/J33 2010183 J1 Tip Adapter
Tip Z6 Board
(2010149)
J34 NPS/J35 2010183 J1 Tip Adapter
Tip Board
Z7 (2010149)
J36 NPS/J37 2010183 J1 Tip Adapter
Tip Board
Z8 (2010149)
J38 GND
J39 GND
SW1/SW2 set to pos “8”
RoMa Freedom Backplane (2010111)
J10 X-motor X-motor
(2010184)
J1 Option 2010156 J15 O-CAN Optibo
CAN Flex 13 Freedom
(2010113)

J2 Local CAN 2010144 ST1/Local RoMa 2


CAN Backplane
(2008783)

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Section 3 Settings

Table 3.14: Cable Connections: From/To

From To
Connection Cable Connection Board
RoMa 2 Backplane (2008783)
ST1 / Local 2010144 J2 Local CAN RoMa Freedom
CAN Backplane
(2010111)
ST9 / Z-Brake Z Brake
(2008782)
ST4 / Z-Motor Z Motor
(2008808)
ST3 / Y-Motor Y Motor
(2008615)
ST6 / Signal 2008815 J4/Signal Gripper
(RoMa) (2008794)
2010143
(GRoMa)
ST5 / Power 2008815 J3/Power Gripper
(RoMa) (2008794)
2010143
(GRoMa)
Gripper (2008794)
J1 / G-Motor 2009372 G Motor
(2009371)
J2 / R-Motor R Motor
(2008809)
J3 / Power 2008815 ST5/Power RoMa 2
(RoMa) Backplane
2010143 (2008783)
(GRoMa)
J4 / Signal 2008815 ST6/Signal RoMa 2
(RoMa) Backplane
2010143 (2008783)
(GRoMa)

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200942-103—September 2007
Technical Data Section 3

Table 3.14: Cable Connections: From/To

From To
Connection Cable Connection Board
1 mL Subsystem
J1 O-CAN 1 2010406 J9 O-CAN 3 Optibo
Freedom
(2010113)
J1 O-CAN 1 2010406 J10 O-CAN 4 Optibo
Freedom
(2010113)
J11 Out 2009610 Heating Plate
(2009625)
J11 Out 2009610 Heating Plate
(2009625)
SMIO-SAFY (2010225)
J2 O-CAN 2010209 J18 O-CAN Optibo
RJ45 Freedom
(2010113)
J3 O-CAN 2010407 J2 O-CAN SMIO
(2009706)
J5/J24 Door 2010178 Door lock 1
(left) (0750270)
J6/J23 Door 2010177 Door lock 2
(right)
(0750270)
SMIO (2009706)
J2 O-CAN 2010407 J3 O-CAN SMIO-SAFY
(2010225)
J18 IN 1 level sensor
system liquid
(2010411)
J19 IN 2 level sensor
system waste
(2010411)
J21 IN 4 level sensor
solid waste
(2010410)

3-30 m2000sp G-series Service Manual


200942-103—September 2007
Section 3 Settings

Table 3.15: Fuses

Fuse Location
Sub-assembly
OPTIBO Board Fuse Size
Protected
(Top to Bottom)
FU1 T 6.3A CU Board
FU6 T 8A Option 1 FaWa
FU7 T 8A Option 2 PosID
FU8 T 8A 1 mL Subsystem
FU9 T 8A 1 mL Subsystem
FU4 T 8A Diluter
FU3 T 8A RoMa/R1 R2
FU2 T 8A LiHa
FU5 T 8A Option 5
FU10 T 8A Arm L1 L2

m2000sp G-series Service Manual 3-31


200942-103—September 2007
3-32
Table 3.16:
LiHa RoMa PosID 2
Parts Qty Motors Pos Address Qty Motors Pos Address Qty Motors Pos Address
Technical Data

619303 5 Y/Ys 1 (Top) 4 2 Y/R Inner 6 2 X Connected to 0


Servo PCB board PosID2 CU board
Z3/Z6 2 3 Z/G Outer 7 Y/B Connected to 2
board Y/B Board
Z2/Z7 3 2
Z1/Z8 4 1
Z5/Z4 5 0
DiTi Eject
Solenoid
626201 1 X 6 5 1 X 0
Servo PWR
PCB
619323 8 1 (Y) 1 (Y)
Motor (Z1-Z8)
619345 1 (R)
Motor
619346 1 (Z)
Motor
619321 2 (Y/Ys) 1 (X)
Motor
619601 1 (B)
Motors, Servo and Servo PWR Boards, and Address Settings

Motor
619854 1 1
X-Drive
Assy

200942-103—September 2007
m2000sp G-series Service Manual
Section 3
Section 3 Diagrams

Diagrams
This section includes all relevant diagrams, such as block
diagrams, wiring diagrams, etc., that are useful for
maintenance or repair.

SMIO/SMIO-SAFY Connection Diagram

Figure 3.10: SMIO/SAFY

m2000sp G-series Service Manual 3-33


200942-103—September 2007
Technical Data Section 3

Wiring Diagrams

Figure 3.11: Instrument Wiring

3-34 m2000sp G-series Service Manual


200942-103—September 2007
Diagrams

3-35
Press 1-1

PIEZO-Module Diluter 1
S-CAN
DIL1
J28
J20

J2

2008556
S-CAN IN

XP 3000+ 2008582 (100)


S-CAN J4
729556
J1

2008582 (150)
Optibo freedom
J12
I/O

2008901 (200)
D1 / #0
Press 1-2

PIEZO-Module Diluter 2
S-CAN
DIL2
J21

J29

J3

2008556 XP 3000+
S-CAN OUT

729556
J13
I/O

D2 / #1
J11
Press 1-3

PIEZO-Module Diluter 3
S-CAN
DIL3
J22

J30

J4

2008556 XP 3000+
729556
J14
I/O

D3 / #2
Press 1-4

PIEZO-Module
VCC-ILID2 (DIL5-8)

Diluter 4
S-CAN
DIL4
J23

J31

J5

2008556 XP 3000+
J39

729556 2010211
ILID freedom
J15
I/O

D4 / #3 J13
Press 1-5

PIEZO-Module Diluter 5

Figure 3.12: Diluter


S-CAN
DIL5
J24

J32

J6

VCC-ILID1 (DIL1-4)

2008556 XP 3000+
729556
J16
I/O

ILID freedom
J38

D5 / #4 2010211
J12
Press 1-6

PIEZO-Module Diluter 6
S-CAN
DIL6
J25

J33

J7

2008556 XP 3000+
729556
J17
I/O

D6 / #5

Pressure2
J37
Press 1-7

PIEZO-Module Diluter 7
S-CAN
DIL7
J26

J34

J8

2008556 XP 3000+
729556
J18
I/O

D7 / #6
LVD-Print
2010212
Press 1-8

PIEZO-Module Diluter 8 J1
S-CAN

LoVol
DIL8
J27

J35

J9

J36
2008556 XP 3000+

200942-103—September 2007
729556
Misc Definition Signals Grounding

J19
I/O
D8 / #7
24V => Power 24V

m2000sp G-series Service Manual


VCC-DILBACK-8 5V => Power 5V Protection Earth
=> Not implemented in this phase AC => Power AC connection
2008555
S-CAN => SystemCan Ground connection:
O-CAN => OptionCan Earth Ground (Chassis) and Power
Ground are connected as follows:
L-CAN => LocalCan
through
=> unshielded connection direct
capacitors
=> shielded connection
Section 3
Technical Data Section 3

Figure 3.13: LiHa

3-36 m2000sp G-series Service Manual


200942-103—September 2007
Section 3 Diagrams

Figure 3.14: RoMa

m2000sp G-series Service Manual 3-37


200942-103—September 2007
Technical Data Section 3

Hood Wiring Diagrams

Figure 3.15: Ballast and ground cable assembly

3-38 m2000sp G-series Service Manual


200942-103—September 2007
Section 3 Diagrams

Figure 3.16: Ballast with connector

m2000sp G-series Service Manual 3-39


200942-103—September 2007
Technical Data Section 3

Figure 3.17: Controller to hood jumper cable assembly

3-40 m2000sp G-series Service Manual


200942-103—September 2007
Section 3 Diagrams

Figure 3.18: Light switch jumper cable

m2000sp G-series Service Manual 3-41


200942-103—September 2007
Technical Data Section 3

Figure 3.19: Power cable assembly

3-42 m2000sp G-series Service Manual


200942-103—September 2007
Section 3 Diagrams

Signal Tower

Figure 3.20: PCB Diagram

m2000sp G-series Service Manual 3-43


200942-103—September 2007
Technical Data Section 3

1. 12 Pos Amp mini universal


mate-n-lock connector plug
housing
2. Amp mini mate-n-lock
connector socket, 18-22
awg, tin crimp
3. Clamp-on ferrite
4. Mount
5. Signal Tower
6. Set screw (M4-0.7 x 4 mm
cup point)
7. Screw (M5-0.8 x 10 mm)

Figure 3.21: Signal tower assembly

3-44 m2000sp G-series Service Manual


200942-103—September 2007
Section 4: Installation

SECTION 4:
INSTALLATION

m2000sp G-series Service Manual 4-1


200942-103—September 2007
Installation Section 4

NOTES

4-2 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
G-series Installation Checklist. . . . . . . . . . . . . . . . . . . . . . . . 4-6
Site Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-13
Pre-Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Cabinet Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-17
Unpacking and Positioning . . . . . . . . . . . . . . . . . . . . . 4-17
Cabinet Leveling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-19
Instrument Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-21
Unpacking the Instrument . . . . . . . . . . . . . . . . . . . . . . 4-22
Lifting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . 4-22
Instrument Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-27
CAN-BUS Resistance Test . . . . . . . . . . . . . . . . . . . . . . . 4-33
Instrument Setup Completion . . . . . . . . . . . . . . . . . . . 4-34
Installation of 1 mL Subsystem . . . . . . . . . . . . . . . . . . 4-39
Hood Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-40
SCC Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-41
Printer Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-42
Installation of Instrument Software . . . . . . . . . . . . . . . . . . 4-43
Hardware Configuration . . . . . . . . . . . . . . . . . . . . . . . . 4-44
Instrument Status Screen . . . . . . . . . . . . . . . . . . . . . . . 4-45
Genesis Setup and Service Software . . . . . . . . . . . . . . . 4-46
Configure the System . . . . . . . . . . . . . . . . . . . . . . . . . . 4-47
Configure the LiHa . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-53
Configure the RoMa . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-74
Configuring Sample Bar Code Labels . . . . . . . . . . . . . . 4-86
Configure the PosID 2 . . . . . . . . . . . . . . . . . . . . . . . . . 4-87
Installation of Wash/Waste Station . . . . . . . . . . . . . . 4-104
Calibrate and Verify System Liquid, Liquid Waste, and
Solid Waste Sensors . . . . . . . . . . . . . . . . . . . . . . . . . 4-105
Reinstallation of the Output Deck . . . . . . . . . . . . . . . 4-115
Perform Peltier Chiller Temperature Check . . . . . . . . 4-115
Check Door Locks . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-115
Verify Temperature Performance of 1 mL Subsystem Heater
Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-119
Set Up Liquid System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-121
FaWa Pump Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-121
FaWa Fill/Flush Protocol . . . . . . . . . . . . . . . . . . . . . . . 4-122
Elimination of Bubbles . . . . . . . . . . . . . . . . . . . . . . . . 4-123
Perform FaWa Pump Test . . . . . . . . . . . . . . . . . . . . . . 4-123
Perform Liquid Detection . . . . . . . . . . . . . . . . . . . . . . 4-127
Perform Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-131
Exiting Setup and Service . . . . . . . . . . . . . . . . . . . . . . 4-133

m2000sp G-series Service Manual 4-3


200942-103—September 2007
Installation Section 4

LiHa Calibrations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-134


Calibrate Tip Adapter Locations . . . . . . . . . . . . . . . . . 4-136
Calibrate Reference Tip Locations . . . . . . . . . . . . . . . 4-139
Sample Rack and Tube Configuration . . . . . . . . . . . . 4-149
Calibrate Disposable Tip Locations . . . . . . . . . . . . . . 4-153
RoMa Calibrations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-157
1 mL Subsystem Calibration . . . . . . . . . . . . . . . . . . . 4-158
Disposable Tip Rack Calibration . . . . . . . . . . . . . . . . 4-161
Shelf Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-165
Calibration Verifications . . . . . . . . . . . . . . . . . . . . . . . . . 4-167
M&D Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-167
Print Calibrations Report . . . . . . . . . . . . . . . . . . . . . . 4-167
Network Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . 4-168
Install the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-168
Network Configuration Screen . . . . . . . . . . . . . . . . . . 4-170
Database Management . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-173
System File Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-173
System File Restore . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-174
On-line Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . 4-176
System Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-177
Audible Alert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-177
Time and Date . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-179
Sample Bar Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-182
Sample Racks and Tubes . . . . . . . . . . . . . . . . . . . . . . . 4-184
Run Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-184
Signal Tower . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-186
Language and Locale . . . . . . . . . . . . . . . . . . . . . . . . . 4-188
Network Configuration . . . . . . . . . . . . . . . . . . . . . . . 4-192
Automatic Export . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-192

4-4 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 Purpose of This Section

Purpose of This Section


This section describes installation of the m2000sp instrument.
It should be carefully reviewed before starting installation.
IMPORTANT: Be sure to verify the instrument you are
! installing is a G-series and not an E-series model. (For
E-series model installation, see the m2000sp E-series
Service Manual.)

Refer to the G-series Installation Checklist, found on page 4–6.


Also, always be sure to check recently released ISAs and TSBs
for updated procedures before beginning any installation.

CAUTION: Personal Injury Risk. Take care to avoid


injury when using sharp tools.
• Do not direct tool points, such as the blade of a
screw driver, toward the face, body, or hands.
• If cutting of tubing, etc., is required, NEVER cut in
the direction of the face, body, or hands.

m2000sp G-series Service Manual 4-5


200942-103—September 2007
Installation Section 4

G-series Installation Checklist


Complete this checklist after each successful installation. Use
the referenced Abbott ISA, TSB, or Manual Section as
more-detailed guides for each step. This checklist should be
filled out by an authorized Abbott FSE only.

Customer:

Customer Contact: System Software Version:

Address: SCC S/N:

City: Country: Instrument S/N:

State: Zip:

Service Technician (print)

Service Technician (sign) Date:

Customer (print)

Customer (sign) Date:

Site Inspection
Task Reference Complete
Pre-installation inspection checklist
Pre-Installation Checklist on page 4–14
obtained and reviewed

4-6 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 G-series Installation Checklist

System Hardware Installation – Cabinet


Task Reference Complete
Cabinet unpacked Unpacking and Positioning on page 4–17

Wheels installed Cabinet Installation on page 4–17

Cabinet placed in desired location Unpacking and Positioning on page 4–17

Cabinet leveled Cabinet Leveling on page 4–19

System Hardware Installation – Instrument


Task Reference Complete
Instrument unpacked Unpacking and Positioning on page 4–17

Instrument placed on cabinet Lifting the Instrument on page 4–22

Unpack accessories Instrument Setup on page 4–27

OPTIBO power connections tightened Instrument Setup on page 4–27

Syringes, valves, and tubing Instrument Setup on page 4–27


connections checked/tightened
LiHa, RoMa, and PosID manually Instrument Setup on page 4–27
checked for freedom of movement
System liquid tubing to FaWa pump FaWa and Pressure Relief Valve Setup on page
and pressure relief valve routed 4–31
CAN-BUS resistance test performed CAN-BUS Resistance Test on page 4–33

1 mL Subsystem cabling routed and Instrument Setup Completion on page 4–34


connected
Sensor cables to SMIO board routed and Instrument Setup Completion on page 4–34
connected
System liquid and liquid waste Instrument Setup Completion on page 4–34
containers and connectors installed
Solid waste container installed Instrument Setup Completion on page 4–34

Worktable, carriers, racks, and 1 mL Instrument Setup Completion on page 4–34


Subsystem labeled
Metal guide pins installed Instrument Setup Completion on page 4–34

1 mL Subsystem installed Installation of 1 mL Subsystem on page 4–39

Ouput Deck installed Latest revision of TSB 605-003


Hood Installed (Optional) The latest revision of TSB 605-018

m2000sp G-series Service Manual 4-7


200942-103—September 2007
Installation Section 4

System Hardware Installation – SCC, Monitor, Printer & Stand


Task Reference Complete
SCC, monitor, printer, and stand SCC Installation on page 4–41
unpacked
Correct SCC voltage verified SCC Installation on page 4–41

System Hardware Installation – Cables


Task Reference Complete
Cables connected to SCC SCC Installation on page 4–41
RS-232 cable between the SCC and the SCC Installation on page 4–41
OPTIBO board connected
Power cords of the SCC, monitor, and N/A
printer connected to the power outlet
Power cord between the instrument N/A
and power outlet connected

System Software Installation


Task Reference Complete
Software installed properly Latest TSB/ISA

Communication between SCC and Installation of Instrument Software on page


instrument verified 4–43
Hardware Configuration screen Hardware Configuration on page 4–44
completed in the m2000sp Software

Setup and Service (S&S) – Setup


Task Reference Complete
Password entered Enter Password on page 4–47

Instrument initialized Initialize the Instrument on page 4–49

Worktable loaded Load Worktable on page 4–50

EEPROM backup of default factory Perform EEPROM Backup on page 4–52


settings performed

4-8 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 G-series Installation Checklist

S&S – Configure the LiHa


Task Reference Complete
Channel configuration performed Channel Configuration on page 4–56

Tip adapter test performed Check Each of the Tip Adapters on page 4–58

LiHa range determined Determine LiHa Range on page 4–60

LiHa reference positions determined Determine LiHa Reference Position on page 4–60

Lower DiTi eject calibrated Calibrate the Lower DiTi Eject on page 4–63

Lower DiTi Eject Test performed Perform the Lower DiTi Eject Test on page 4–64

LiHa Y-spread procedure performed Y-Spread Procedure on page 4–67

S&S – Configure the RoMa


Task Reference Complete
RoMa setup performed Set up the RoMa on page 4–75

RoMa reference positions determined Determine RoMa Reference Positions on page


4–78
RoMa range determined Determine Range on page 4–81

Auto-adjustment of the rotator Adjust the Rotator on page 4–82


performed
Manual adjustment of the rotator Manual RoMa Rotator Adjustments on page
performed 4–83

S&S – Configure the PosID


Task Reference Complete
PosID functionality test performed PosID Functionality Test on page 4–87

Basic mechanical accuracy of the PosID Verify Basic Mechanical Accuracy on page 4–91
confirmed
PosID 2 setup performed Set Up PosID 2 on page 4–92

m2000sp G-series Service Manual 4-9


200942-103—September 2007
Installation Section 4

S&S – Additional Instrument Setup


Task Reference Complete
Wash/waste station installed Installation of Wash/Waste Station on page
4–104
Output Deck replaced Reinstallation of the Output Deck on page 4–115

S&S – Sensors, etc.


Task Reference Complete
Chiller temperature check performed Chiller and Heater Temperature Check, found
on page 5–52 of Section 5, Maintenance and
Diagnostic Procedures.
System liquid, liquid waste, and solid Calibrate and Verify System Liquid, Liquid
waste sensors calibrated Waste, and Solid Waste Sensors on page 4–105
Door locks verified Check Door Locks on page 4–115

1 mL Subsystem temperature check Verify Temperature Performance of 1 mL


performed Subsystem Heater Blocks on page 4–119

S&S – Liquid System Setup


Task Reference Complete
FaWa pump set up FaWa Pump Setup on page 4–121

Fill/flush protocol performed FaWa Fill/Flush Protocol on page 4–122

FaWa pump test performed Perform FaWa Pump Test on page 4–123

Liquid detection test performed Perform Liquid Detection on page 4–127

S&S – Finish Setup


Task Reference Complete
EEPROM backup performed Perform Backup on page 4–131

Configure Sample Racks and Tubes


Task Reference Complete
Configure sample racks and tubes Sample Rack and Tube Configuration on page
4–149

4-10 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 G-series Installation Checklist

Calibrations – LiHa
Task Reference Complete
Tip Adapter calibrations performed Calibrate Tip Adapter Locations on page 4–136

Reference Tip calibrations performed Calibrate Reference Tip Locations on page 4–139

Disposable Tip calibrations performed Calibrate Disposable Tip Locations on page


4–153

Calibrations – RoMa
Task Reference Complete
Calibration of the 1 mL Subsystem 1 mL Subsystem Calibration on page 4–158
performed
Calibration of the DiTi carrier racks Disposable Tip Rack Calibration on page 4–161
performed
Calibration of the DiTi shelves Shelf Calibration on page 4–165
performed

Verification of Installation – Full Backup


Task Reference Complete
Full backup performed System File Backup on page 4–173

Verification of Installation – M&D Procedures


Task Reference Complete
Extensive flush performed Extensive Flush on page 5–10 of Section 5,
Maintenance and Diagnostic Procedures
50 µL Dispense Gravimetric Test Dispense Test 50 µL, Gravimetric on page
performed 5–23 of Section 5, Maintenance and
Diagnostic Procedures
800 µL Dispense Gravimetric Test Dispense Test 800 µL, Gravimetric on page
performed 5–26 of Section 5, Maintenance and
Diagnostic Procedures
LiHa Location Test performed LiHa DiTi Location Test on page 5–12 of
Section 5, Maintenance and Diagnostic
Procedures
LiHa Sample Tube Check performed LiHa Sample Tube Check on page 5–14 of
Section 5, Maintenance and Diagnostic
Procedures

m2000sp G-series Service Manual 4-11


200942-103—September 2007
Installation Section 4

DiTi Rack Tests – Deck and Shelf RoMa DiTi Rack Test on page 5–27 and
performed RoMa DiTi Shelf Test on page 5–29 of
Section 5, Maintenance and Diagnostic
Procedures
RoMa 1 mL Carrier Test performed RoMa 1 mL Carrier Test on page 5–30 of
Section 5, Maintenance and Diagnostic
Procedures
Sample Extraction Test Sample Extraction Test on page 5–37 of
Section 5, Maintenance and Diagnostic
Procedures
Z-Calibration Check (Sample Z-Calibration Check – Sample Extraction on
Extraction and Master Mix Addition) page 5–40 and Z-Calibration Check – Master
performed Mix on page 5–45 of Section 5,
Maintenance and Diagnostic Procedures

Network Configuration (if applicable)


Task Reference Complete
Router installation Network Configuration on page 4–168

AbbottLink installation See the latest revision of the AbbottLink ISA

On-Line Documentation
Task Reference Complete
On-line documentation loaded Refer to the Software Updates page on the
GSS website

4-12 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 Site Inspection

Site Inspection

The pre-installation site inspection involves the following


activities:
• Confirming site specifications, as listed on the
Pre-Installation Checklist
• Confirming the viability of the delivery route by
walkthrough; refer to Dimensions and Weight on page
3–10 of Section 3, Technical Data
• Confirming adequate power is available; refer to
Environmental Conditions on page 3–13 of Section 3,
Technical Data
• Confirming proper environmental conditions exist; refer
to Environmental Conditions on page 3–13 of Section 3,
Technical Data
• Confirm that the Miscellaneous requirements, as listed on
the Pre-Installation Checklist, are met
• Complete the Pre-Installation Checklist on page 4–14

m2000sp G-series Service Manual 4-13


200942-103—September 2007
Installation Section 4

Pre-Installation Checklist
Account Information

Account Name
Delivery Address Bldg. & Floor
City State/Country Zip Code
Primary Contact Phone
Secondary Contact Phone
Customer Number Customer E-mail

Site Specifications
Instrument & Cabinet space meets specification criteria
Record space
measurements _____________ Length
_____________ Height
_____________ Depth

Specifications – 176.5 cm (69.5 in) instrument and cabinet length


(side service door open). 61 cm (24 in) are required for
clearance on each side.
298.5 cm (117.5 in) total length
– 203 cm (80 in) instrument and cabinet height (RoMa
extended).
168 cm (66 in) total height (245 cm/96 in w/hood)
– 79.4 cm (31.3 in) instrument and cabinet depth. 10.2
cm (4 in) of clearance is required in back and 91.4 cm
(36 in) is required in front.
196.2 cm (77.3 in) total depth
Is instrument or cabinet blocking a thoroughfare? ___ Yes ___ No
Will an operator using the instrument block a ___ Yes ___ No
thoroughfare?
Is the space provided for commodities inadequate? ___ Yes ___ No
If any of the answers above were YES, consult with the customer on an alternate installation location.
Action plan to resolve:

Weight – 293 kg (645 lb) instrument, cabinet, and accessories (310 kg/682 lb w/hood)
Notify customer the floor load capacity must be verified to see if it is adequate
If floor capacity is inadequate, resolution plan:

System Control Center space meets specification criteria


Record space measurements: ____________ Length ____________ Height ____________ Depth
Specifications: Length: 70 cm (24 in) + Height: 162.6 cm (64 in) + Depth: 78.7 cm (31 in) ++++ Weight: 27.3 kg (60 lb)

Page 1 of 3

4-14 m2000sp G-series Service Manual


200942-103—September 2007
Section 4 Pre-Installation Checklist

Delivery Route
Measure door width openings from entryway to lab and record here: _________________________________
Check one: _____ Door and entryway openings are 102.9 cm (40.5 in) or wider
_____ Door and entryway openings are less than 102.9 cm (40.5 in) but at least 81.5 cm (32 in). In this case,
REMOVAL OF INSTRUMENT FROM PALLET MAY BE REQUIRED. Contact area customer service.
_____ Door and entryway openings are less than 81.5 cm (32 in). Contact area customer service.
Measure turning radius in passageways. The m2000sp requires a combined 279.4 cm (110 in) of width of both passageways
around corners.
Is there a loading dock? Yes _____ No _____ If No, contact area customer service
Does the loading dock have a raised delivery platform? Yes _____ No _____ If No, contact area customer service
If any of the following are answered YES, contact area customer service
Does the instrument have to be moved up or down stairs? Yes _____ # of flights __________ No _____
Does the instrument have to be moved via freight elevator? Yes _____ Elevator door width __________ No _____
Will the instrument have to be lifted over any barriers? Yes _____ Height of barrier __________ No _____

Resolution plan (if necessary):

Power Requirements
Dedicated/Isolated 15 amp power line with circuit breaker identified as Abbott m2000sp System is optimal. If not possible,
how clean is the power? What other equipment is plugged into the same power line? See Uninterruptible Power Supply below.
m2000sp power outlet is within 30.5 cm (12 in) of the instrument’s electrical input panel.
Power Measurements
System Requirements: Voltage range: 100-240 VAC + 10%
NOTE—All system components Voltage frequency: 50/60 Hz + 1%
will run at the single phase voltage
standard for the country (e.g. Power: 1200 VA
Japan 100VAC + 10%, US 110VAC Circuit breaker: 15 A
+ 10%, EU 220VAC + 10%)

Line Noise: Breaker Closed Open


Line to neutral 100-240 VAC + 10% < 0.5 VAC
Line to earth 100-240 VAC + 10% < 0.5 VAC
Neutral to earth < 0.5 VAC < 0.5 VAC
NOTE—If the power is not within specification, have the customer notify their electrician for correction
Four (4) outlets provided (instrument, computer, printer, and monitor) _______ Yes _______ No
NOTE—If four outlets are not available, have the customer notify their electrician to add additional outlets

Site Environmental Requirements


Operation temperature: 15-30 C (59-86 F) Operating altitude: Max 2000 m (6600 ft)
Operation humidity: 30-80% relative (non-condensing) Operating noise: < 80 dBA measured @ 1 m
@ 30 C (86 F) or below
Pollution degree: 2 BTU output: 4100 BTU / 1200 WA
Over Voltage Category: II NOTE—Avoid placing instrument near air vents or drafts

Page 2 of 3

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200942-103—September 2007
Installation Section 4

Miscellaneous
System Liquid Requirements: – Internal waste container used for liquid waste recovery
– Non-plumbed distilled or deionized water is required
Check bar code type below (check all that apply)
Codabar _____ w/o Checksum Code 39 standard _____ w/ Checksum _____ w/o Checksum UPCA _____
Interleaved 2 of 5 _____ w/ Checksum _____ w/o Checksum Code 128 subset A, B, and C _____ Code 93 Standard ____
Unknown _____ Other _____ Please list ________________________________ OBTAIN A BARCODE SAMPLE
Record the type(s) of sample tube(s) that have been chosen by the customer
Type of sample tube __________________________________ Distributor/Manufacturer _________________________________
Sample tube size _____________________________________ Catalog number __________________________________________
OBTAIN one (1) of each sample tube being used by the customer
Uninterruptible Power Supply (UPS) Requirements
It is recommended that the customer use one of the following UPS:
— For the m2000sp G-series: General Electric GT1500 (110V); for 220V, contact the EMEA Support team in Germany
— For the SCC: 07D06-11 (110V) or 07D06-22 (220V)
Additional Questions
What kind of samples will be used (blood, buffer, urine, etc.)? __________________________________________________________
Will the customer be performing DNA or RNA extractions? _____________________________________________________________
Does the customer require a certificate of installation (IQ/OQ)? _________________________________________________________

Action plan to resolve inadequacies or concerns:

Refer to the m2000sp Service Manual for more detail on any of the above information.

Customer Signature/Title and Date

Abbott Representative Signature and Date

Fax completed report to: m2000sp Product Manager


1.224.361.7505

Questions? Call: 1.224.361.7375 or, if in Europe, the Middle East, or Africa, contact the EMEA
Service and Support team in Germany
Page 3 of 3

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Section 4 Cabinet Installation

Cabinet Installation

Cabinet installation involves the following activities:


• Unpacking the cabinet
• Installing the wheels
• Placing the cabinet in the desired location
• Leveling the cabinet

Unpacking and Positioning


The m2000sp instrument is delivered in multiple boxes. The
boxes contain the m2000sp instrument, cabinet, SCC, SCC
support stand, printer, and monitor. Before unpacking the
instrument, ensure all parts and packaging are visually
inspected for possible damage sustained during transportation.
Verify the shipment according to the packing list.
NOTE: Save and store packaging material for possible
future shipment of the instrument.

1. The cabinet is packed in the smaller of the two large boxes.

Figure 4.1: Cabinet and Instrument Boxes

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Installation Section 4

2. Remove the banding and lift off the top.

Figure 4.2: Bands and Top Cover Removed from Box

3. Remove the cardboard box from the pallet.


WARNING: Lifting Hazard. Four people are
required to lift the cabinet. Use proper lifting
technique.
4. Move the cabinet to the edge of the pallet and rotate onto
its back as shown.

Figure 4.3: Cabinet Rotated for Removal of Legs

5. Remove the original screw-adjustable legs and discard.


6. Install the supplied mounting bolts onto the caster before
installing on the cabinet.
7. Carefully thread the mounting bolt into the cabinet base
and tighten.

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Section 4 Cabinet Installation

8. Position the leveling block at the maximum raised


position. Ensure that the squared top is level with the
cabinet bottom.

Figure 4.4: Leveling Block Raised to Top Position

9. Ensure the caster is properly secured to the cabinet.


10. Rotate the cabinet onto the casters; move to allow the
removal of the shipping pallet.
11. Place the cabinet in the desired location. Refer to Table
3.11: Site Requirements on page 3–14 of Section 3,
Technical Data.

Cabinet Leveling
1. Level the cabinet by performing the following (refer to
Figure 4.5 below):
• Front to back (A-B): Place a 1.22 m/4 ft bubble level on
the end of the cabinet, parallel to A-B. Adjust the
caster on the low corner until the bubble is centered
in the sight glass.

Figure 4.5: Cabinet Leveling Positions

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Installation Section 4

• Front to back (C-D): Place level on the end of the


cabinet, parallel to C-D. Adjust the caster on the low
corner until the bubble is centered in the sight glass.
• Front to back (E-F): Place level on the middle of the
cabinet, parallel to E-F. Adjust the caster on the low
side until the bubble is centered in the sight glass.
2. Verify the entire cabinet is level by placing the level on all
positions noted above: A-B, C-D, E-F, B-F-D, and A-E-C.

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Section 4 Instrument Installation

Instrument Installation

Instrument installation involves the following activities:


• Unpacking the instrument
• Placing the instrument on the cabinet
• Unpacking the accessories
• Tightening the OPTIBO power connections
• Checking/tightening the syringes valves and tubing
connections
• Manually checking the LiHa, RoMa, and PosID for
freedom of movement
• Routing and connecting the system liquid tubing to the
FaWa pump and pressure relief valve
• Routing and connecting the 1 mL Subsystem cabling
• Performing the CAN-BUS resistance test
• Routing and connecting the sensor cables to the SMIO
board
• Installing the system liquid and liquid waste containers
and connectors
• Installing the solid waste container
• Labeling the worktable, carriers, racks, and 1 mL
Subsystem
• Installing metal guide pins
• Installing the 1 mL Subsystem
• Installing the Worktable Kit (including the Output Deck)
per the latest revision of TSB 605-003
• Installing the hood per the latest revision of TSB 605-018, if
required
• Unpacking and installing the SCC, monitor, printer, and
stand
• Connecting the SCC and OPTIBO board via cable
• Connecting the power cords from the instrument and SCC
to appropriate power outlets

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Installation Section 4

Unpacking the Instrument


1. Remove the bands, cardboard top, and box from around
the instrument.

Figure 4.6: Large Box in Instrument

2. Remove the large brown box containing the 1 mL


Subsystem and other accessories.

Lifting the Instrument


Perform the following steps to mount the instrument to the
top of the cabinet:
1. Ensure the lifting device is ready.
NOTE: The lifting device includes four handles
(two left and two right), two pipes, and four safety
pins.

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Section 4 Instrument Installation

2. Set up the lifting device according to the following


illustrations.

3 4
1 2
5

1 Left lifting bracket 4 Frame strut


2 Instrument worktable 5 Safety pin
3 Instrument screws 6 Pipe
Figure 4.7: Lifting Assembly

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Installation Section 4

a. Lifting brackets are left- and right-side specific. Note


that “R” is stamped on the right lifting bracket.

Figure 4.8: Right Side

b. This side view illustrates placement of the lifting


bracket.

Figure 4.9: Lifting Bracket Placement

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Section 4 Instrument Installation

c. The bracket locks against screws underneath the


instrument, holding the bracket in place so it does not
slip out.

Figure 4.10: Bracket Locking Against Screws

WARNING: To avoid injury, ensure the safety pins


are placed according to the following illustration.

d. Safety pins secure the handles in place.

Figure 4.11: Safety Pins in Place

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Installation Section 4

Figure 4.12: Right Side Lifting Handle in Place

WARNING: The instrument is heavy. Four people


are required to carefully lift the instrument onto
the cabinet. While lifting, ensure the lift handles
remain engaged with the locking features of the
instrument.
3. Center the instrument on the left and right sides of the
cabinet. Ensure the edges of the rear feet are flush with the
back edge of the cabinet.

Figure 4.13: Lifting Up

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Section 4 Instrument Installation

Instrument Setup
1. Remove the styrofoam blocks from the top ends of the
instrument, the two cardboard worktable protection
strips, styrofoam blocks wedged between the diluters and
hotel, and the cardboard pieces protecting the X-rail and
PosID.

Figure 4.14: Packing Material Shown

2. Remove the styrofoam blocks protecting the LiHa and


RoMa.

Figure 4.15: Styrofoam Blocks Protecting LiHa and RoMa

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Installation Section 4

3. Remove the styrofoam pieces supporting the PosID.

Figure 4.16: Styrofoam Pieces Supporting PosID

4. Remove the left- and right-side transport support brackets,


each held in place with two screws (M2.5).

Figure 4.17: Transport Support Bracket

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Section 4 Instrument Installation

5. Remove the tape and packing material from the left and
right sides of the cabinet.

Figure 4.18: Packing Material in Cabinet

6. Unpack the accessories, including the carriers, racks,


bottles, etc.
7. Remove the screw (M6 x 20) and nut located on the left
side of the cabinet in the bottom rail. The screw prevents
movement of the waste drawer during shipment. Reinstall
the screw in the middle vertical support rail, as shown
below. The screw acts as a stop to prevent over-flexing of
the left cabinet door. Ensure the screw does not interfere
with movement of the waste drawer guide rail.

Figure 4.19: Placement of Cabinet Screw

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Installation Section 4

8. Tighten all screw terminals on the OPTIBO (left access


door).

Figure 4.20: OPTIBO Board

9. Tighten each syringe firmly to the valve and the two


tubing connections to each valve.
10. Finger-tighten tubing connections on the 1:4 distributors
(upper access door).

Figure 4.21: 1:4 Distributor Valves

11. Manually check movement of the LiHa, PosID, and RoMa


for any obstruction.

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Section 4 Instrument Installation

FaWa and Pressure Relief Valve Setup


IMPORTANT: Ensure the arrows on the Pressure Relief
! Valve point in the direction of fluid flow.
1. Remove the two outer screws from the FaWa assembly
located on the left side of the instrument. Pull out the
FaWa assembly to access the FaWa pump and Pressure
Relief valve.
2. Remove any existing tubing from the input side of the
FaWa pump and output side of the Pressure Relief valve
only.
3. Route the one clear plastic tube up through the hole in the
cabinet deck, through the hole in the worktable surface,
under the worktable, and around the back of the FaWa
assembly to the left side of the FaWa assembly. Connect
the tubing to the Pressure Relief valve and secure with a
screw clamp. Ensure the Pressure Relief valve is angled in
the direction as shown to prevent crimped tubing.

Arrows pointing in
the direction of
flow.

Figure 4.22: Tubing Connected to Pressure Relief Valve

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Installation Section 4

4. Route the second length of tubing to the front of the FaWa


assembly, and up through the gap between the FaWa
assembly and instrument frame (behind the Waste Front
Blind cover). Connect the tubing to the input side of the
FaWa pump and secure the tubing to the pump with a
spring clamp.

Figure 4.23: Tubing Routed and Connected to FaWa Pump

NOTE: The tubing may be shortened to remove


excess slack.
5. Connect the two cables for the 1 mL Subsystem to CAN
Option 3 and 4 on the OPTIBO board. Secure the cables to
the instrument frame with the supplied cable clamps, two
per cable.

Figure 4.24: OPTIBO

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Section 4 Instrument Installation

CAN-BUS Resistance Test


NOTE: Power to the instrument must be off when
measuring resistance.
1. Measure the CAN-BUS resistance on the OPTIBO (left
access door):
a. Open the left service access door.
b. Measure the CAN-BUS resistance with an ohmmeter
on the two checkpoints, CAN-L and CAN_H for
system CAN-BUS, and CANO-L and CANO_H for
option CAN-BUS on the OPTIBO as shown.

Figure 4.25: CAN-BUS Resistor Check Points on OPTIBO

NOTE: Ensure the instrument is switched off while


measuring CAN-BUS resistance. If the resistance is not
50 to 65 Ω, refer to Quick Check for Jumper Settings on
page 9–207 of Section 9, Repair, Replacement of Spare
Parts to determine jumper settings.

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Installation Section 4

Table 4.1: CAN-BUS Resistance

CAN-BUS Check Points Resistance Interpretation

System CAN-L/CAN-H 50-65 Ω Correct number of


CAN-BUS jumpers
~ 40 Ω 1 jumper too many
~ 120 Ω 1 jumper too few
Option CANO-L/ 50-65 Ω Correct number of
CAN-BUS CANO-H jumpers

~ 40 Ω 1 jumper too many


~ 120 Ω 1 jumper too few

2. Power on the instrument. Reestablish communication


with the instrument and initialize the instrument.

Instrument Setup Completion


After completing the CAN-BUS Resistance Test, continue with
instrument setup as follows.
1. Route the 1 mL Subsystem cables in front of the FaWa
pump as shown, and underneath the worktable surface.

Figure 4.26: 1 mL Subsystem Cable Routing

2. Notch the sealing strip at grids 10 and 15 for the two 1 mL


Subsystem cables. Route the 1 mL Subsystem cables
through the notched holes; leave enough cable length to
connect to the 1 mL Subsystem. Notch the cabling at grid
41 for the chiller cable.
3. Ensure that none of the tubing or cabling is crimped, and
that it does not interfere with the movement of the PosID
assembly.

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Section 4 Instrument Installation

4. Feed any extra cable length in front of the instrument.


Secure the FaWa assembly to the instrument frame with
two screws.

Figure 4.27: Cable Routing through Sealing Strip

5. Route the three sensor cables (system liquid, liquid waste,


solid waste) through the opening in the top of the cabinet
and underneath the instrument.

Figure 4.28: Sensor Cables between Instrument and Cabinet

6. Route the cables outside the frame of the instrument


covered by the right side service door. Be careful not to
damage the cables by pulling them against the edges of the
frame.

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Installation Section 4

7. Secure the cables to the front frame with cable clamps, two
per cable. Use a flathead screwdriver to carefully pry the
clamps from the frame.

Figure 4.29: Cables Secured to Front Frame

8. Attach the cables to the SMIO board:


a. Plug the cables into the connectors on the top SMIO
board.
b. Plug the system liquid sensor (green-taped cable) into
J18, the connector farthest to the right next to the
CAN-BUS jumper.
c. Plug the liquid waste sensor (red-tipped cable) into
J19, the connector second from the right next to the
system liquid sensor cable connector.
d. Skip one connector and plug the solid waste sensor
cable into J21.
Refer to the drawing on the inside of the right service
access door.

Figure 4.30: Sensor Cable Connected to SMIO Board

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Section 4 Instrument Installation

9. Apply a green decal to the waste tray for the system liquid
container and a red decal to the waste tray for the liquid
waste container.

Figure 4.31: Decals Applied to Waste Tray

10. Apply a green decal to one face of the system liquid


container and a red decal to the face of the liquid waste
container.

Figure 4.32: Decals Applied to Liquid Containers

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Installation Section 4

11. Install the system liquid container (filled with distilled or


deionized water) into the left container compartment
(liquid level sensor closest to the floor). Connect the lid,
with a white quick-connect fitting and clear tubing, to this
container.
NOTE: Seams of the bottle should face away from
the sensor.

Figure 4.33: System Liquid Cap and Connections

12. Install the liquid waste container into the right container
compartment. For ease of identification, tie-strap the two
liquid waste tubes together. Then, connect the clear
tubing with the beige quick-connect fitting to the
container cap’s beige male connection and the clear
tubing with the white quick-connect to the white male
connection.
NOTE: Seams of the bottle should face away from
the sensor.

Figure 4.34: Waste Liquid Cap Connection

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Section 4 Instrument Installation

13. Install the solid waste container, with bag for disposable
tips, into the cabinet.

Figure 4.35: Solid Waste Container

14. Perform installation of the m2000sp worktable kit per the


latest revision of TSB 605-003.

Installation of 1 mL Subsystem
1. Power off the instrument.
2. Place the 1 mL Subsystem upside down on the worktable
surface. Connect the two cables to the two heater boards,
accessed through the notched opening in the bottom
cover of the 1 mL Subsystem; the cables can be connected
to either of the two connectors. The software reads the
address setting of each heater block. Secure the cables with
a screwdriver.

Figure 4.36: 1 mL Subsystem, Bottom View

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Installation Section 4

3. Align the left edge of the 1 mL Subsystem with location


grid 7 and place the 1 mL Subsystem on the deck, aligning
the notches in the middle guide bar on the bottom of the
1 mL Subsystem with the guide pins on grid 12.

Figure 4.37: 1 mL Subsystem location

4. Push extra cable length through the notched openings in


the sealing strip. Ensure the 1 mL Subsystem is in the
correct location, held in place on the worktable by the
positioning pins and the corresponding notches in the
middle guide bar on the bottom of the 1 mL Subsystem.
5. Close the front worktable cover.
6. Lift the 1 mL Subsystem off the worktable and set on the
ground. Leave cables connected.
NOTE: The wash/waste station and Output Deck
are installed after the worktable surface is defined
and the PosID, LiHa, and RoMa are calibrated.

Hood Installation
Hoods are not standard equipment on all G-series instruments.
When installation is required, refer to the latest revision of TSB
605-018.

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Section 4 Instrument Installation

SCC Installation
1. Unpack the computer stand, SCC, monitor, printer,
keyboard, and mouse. Set up the computer stand then set
the SCC, monitor, printer, keyboard, and mouse in place.
Do not connect cables at this time.

Monitor

Computer (CPU)
with CD Drive

Keyboard

Mouse

Figure 4.38: SCC and Stand

2. Plug all power cables into the rear of the SCC as shown
below. Verify the correct voltage is selected on the SCC.

Figure 4.39: Rear of SCC

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Installation Section 4

3. Connect the RS-232 cable, shipped with the instrument,


between the OPTIBO board and SCC. Connect the power
cord to the instrument.

Figure 4.40: RS-232 Cable Connection

4. Plug the instrument power cord into the power outlet.

Printer Installation
Refer to the latest revision of the applicable printer or software
installation TSB.

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Section 4 Installation of Instrument Software

Installation of Instrument Software

The keys to successful software installation are:


• Referring to the latest revision of the software TSB and/or
ISA for detailed software installation instructions
• Verifying the software version to be installed is for the
correct SCC platform (SCC series D software is not
compatible with an SCC RAD945)
• Following installation of the software, verifying that the
instrument is connected via the RS-232 cable to the
computer
IMPORTANT: The 1mL Subsystem cables must be
! connected to the subsystem and OPTIBO for the
instrument to reach the STOPPED state. Otherwise, it
will remain in the CONNECTING state for a time and
then switch to OFFLINE. Continuing with software
configuration while the instrument is in this state
might corrupt the software.
Note that if the RS-232 cable is disconnected while the
software is initializing, the software will lock up until the cable
is replaced. Also verify that the chiller is connected to the USB
Edgeport per the TSB and is powered on.
When communication is established with the instrument, the
following parameters are gathered:
• Firmware Version
• Serial Numbers
• Offsets, Ranges, Scale Factors
• Available Options
• Worktable Database is Opened

Proceed with Hardware Configuration, found on page 4–44.


Refer to System Settings on page 4–177 for a discussion of
other configuration and setting options.

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Installation Section 4

Hardware Configuration
From the <System Settings> menu, select <Hardware
Configuration> then <Configure>.

Figure 4.41: Hardware Configuration screen

1. Enter the instrument serial number in the appropriate


field.
2. In the <Type> field, select <G-series>.
3. The <External Devices> section provides a listing of any
external devices installed with that instrument, and
indicates whether they have been enabled. The signal
tower listed here is part of the hood configuration.
If a hood has been installed on the instrument, ensure the
<Signal Tower> has been enabled with a check mark.
If no hood is to be installed, ensure there is no check
mark next to <Signal Tower>.
4. Select <Save>. The instrument will reboot if the settings
(other than serial number) have changed.
If the signal tower was enabled, it can be configured after
the reboot. Refer to Signal Tower on page 4–186.

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Section 4 Installation of Instrument Software

Instrument Status Screen


The Instrument Status screen provides the current status of
the instrument.
Table 4.2: Instrument Status

Status Status Definition


Offline Software and instrument not
communicating. Instrument off or not
connected.
Connecting Software and instrument are attempting to
communicate via RS-232.
Initializing The instrument is performing a system
initialization from a stopped state.
Stopped The instrument is in an uninitialized and
unknown (not homed) state.
Ready The instrument is in a known (homed)
state, and is ready to execute a run.
Running The instrument is running an application.
* Paused An application or maintenance procedure
has been paused by the user.
* Paused: An immediate pause has been enacted by
Immediate the user.
Run The instrument is preparing for a run.
Initialization

* The <Pause> option stops an in-process application run as


soon as the instrument reaches the next pipetting step
(homing the LiHa and RoMa) and allows the user to update
the tip status, if necessary. The <Immediate Pause> option
instantly stops the instrument, regardless of what activity is
being performed or what stage the run is in.

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Installation Section 4

The <Instrument Status> screen also provides an update of


the current status of the hardware, as well as the status of an
in-process application. The hardware status includes sensors,
heaters, and major mechanisms, as shown below. A status of
<Tripped> is caused when the sensor has been tripped.
Table 4.3: Status Options

System Status Options


Liquid Waste OK, Tripped
Solid Waste OK, Tripped
Systems Fluid OK, Tripped
Doorlocks (L/R) Locked, Unlocked
Heater Zone 1 Failed, Current Temperature
Heater Zone 2 Failed, Current Temperature
Chiller Failed, Current Temperature
LiHa Failed, OK
RoMa Failed, OK
FaWa Failed, OK
PosID Failed, OK

Genesis Setup and Service Software


Genesis Setup and Service software is used for field service
troubleshooting, instrument setup, and diagnostics. See
Section 11, Genesis Setup and Service Software for detailed
information on the software features not covered in the
installation process.
In order to navigate to setup and service software from the
m2000sp application software, log in to the application as an
FSE. Navigate to <System>, <Setup and Service>.

Figure 4.42: Route to Setup and Service

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Section 4 Installation of Instrument Software

Configure the System

Enter Password
1. Start the function with <System>, <Password>. The
<Password> dialog displays.
Password Protection: Enter the password to
access specific service-related software menu
items.
NOTE: The <System> menu includes all
commands that apply to the whole system, and
not to a particular function.

Figure 4.43: System Menu

2. Enter the field service password (smart) in the <Enter


Password> field.

Figure 4.44: Password Dialog

3. Confirm the entry with <OK>. FSE functions are enabled


in menus and screens.

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Installation Section 4

COM Port

Purpose
This function allows the communication port (e.g., COM1,
COM2, etc.) to be selected or changed. The computer
communicates with the instrument through the
communication port.
NOTE: It should not be necessary to change the port
assignment. Installation uses COM1.

Procedure
Perform the following steps to select or change a
communication port.
1. Ensure the instrument is connected to the required COM
port connector, normally located on the rear side of the
computer. Refer to the accompanying documentation for
the computer, if necessary.

Figure 4.45: COM Port Sub-menu

2. From the System <COM port> sub-menu, select the


correct port (COM1, COM2, etc.) The sub-menu lists all
available COM ports.
NOTE: In the sub-menu, the currently selected
COM port is marked with a check (⻫).
NOTE: Depending on the selection, the computer
automatically leaves or enters the simulation
mode.

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Section 4 Installation of Instrument Software

Initialize the Instrument


1. Select <System>, <Initialize Instrument>.

Figure 4.46: System Menu

2. The message <Initialize system. Please wait...> displays.

Figure 4.47: Initialize System

NOTE: During initialization, all installed system


devices (PosID, LiHa, RoMa) are moved to their
initial positions.
3. The message dialog disappears when initialization is
complete.

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Load Worktable
1. To load a worktable, select <System>, <Load Worktable>.
Refer to Load Worktable in Section 11, Genesis Setup
and Service Software for more information.
2. If available, select the <m2000sp Worktable>. Otherwise,
select the <NAT MBS Worktable>.

Figure 4.48: Select Worktable Dialog

3. Select <OK>.

Test Configuration (Not Currently Used)


1. The Test Configuration option is used to load any test
format configured for a test or protocol, by selecting Save
for *.ini files or Save As for *.any files.
2. Start test configuration by selecting System > Test
Configuration.
3. The Open Configuration dialog displays.
4. Select the file type in the Files of Type list.
5. Select the appropriate file.
6. Load the file with Open.

Print Setup (if required)


1. Perform Print setup by selecting <System>, <Print Setup>.
This command starts the standard Windows dialog to set
up a printer.
2. Select <Okidata B4350> as the printer.

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Print All Configuration Data (Not Required)


1. Select <System>, <Print>, <Configuration>.
2. The <Common Print Information> dialog displays.

Figure 4.49: <Common Print Information> Dialog

3. Select the print destination and fill out the <Header


Information>.
4. Start immediate printing with <OK>.

Print Specific Data (Not Required)


1. Select <Print> on the corresponding assembly screen (e.g.,
LiHa) to print configuration data and/or test results of a
specific function after any test was run.
2. Select the print destination(s) and fill out the Header
Information as above.

Figure 4.50: <Test Specific Print Information> Dialog

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3. Confirm entries with <OK>. The <Test Specific Print


Information> dialog displays.
4. Select the data to print and enter any comments.
5. Start printing with <OK>.

Perform EEPROM Backup


Save the default factory settings of the m2000sp instrument by
performing a backup of the EEPROM parameters. Refer to
EEPROM Backup, found on page 4–132.

Exit/Finish Setup
Select <System >, <Exit> to close the Setup & Service software
safely without data loss.

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Configure the LiHa


LiHa configuration in S&S involves the following activities:
• Performing channel configuration
• Performing the Tip Adapter Test
• Determining the LiHa range
• Determining the LiHa reference positions
• Calibrating the lower DiTi eject
• Performing the Lower DiTi Eject Test
• Performing the LiHa Y-spread procedure

WARNING: Potential Biohazard. Indicates an area


where the FSE or operator may be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator may
be exposed to hazardous chemicals when handling
Abbott Molecular sample preparation reagents.
Exposure to hazardous chemicals is minimized by
following instructions provided in product-specific
labels and product-specific Material Safety Data Sheets
(MSDS). Wear appropriate personal protective
equipment, such as gloves, lab coat, and protective
eyewear.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
CAUTION: Ensure there are no objects installed on the
worktable that could interfere with movement of the
LiHa. It may be necessary to restart the software and
re-enter the password, smart, if the software was exited.

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WARNING: Pinch Point. The instrument requires


accurate positioning of system modules, and carriers
and racks on worktables. While working with the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Keep back from the worktable surface during these
procedures.
• Fast moving devices could cause injuries.
NOTE: The SN of the LiHa is not stored in the software.
A prompt to enter the SN displays when either cycling
power to the instrument or performing a <Setup> task
without first entering the SN. Select <Apply>.

LiHa Move Tool


To perform many of the procedures in this section, it will be
necessary to use the LiHa Move Tool. Refer to Move Pages on
page 11–17 of Section 11 for instructions on using this
feature.

Reference Tips
Several LiHa configuration activities require the use of a
reference tip. There are currently two types of reference tips
available:
• Long reference tip (old style)
• Short reference tip (new style)

Figure 4.51: Reference Tips

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Either reference tip can be used to perform all applicable


configurations and calibrations; however, the procedures
required to mount the different tips differ significantly, as
described under Mounting the Long Reference Tip and
Mounting the New Short Reference Tip.

Mounting the Long Reference Tip


1. Using the DiTi cone wrench, turn DiTi cone 3 clockwise to
loosen and remove it from the tip ejector tube.
NOTE: The DiTi cone is screwed into the tip
grabbing assembly through a right-threaded screw.

Figure 4.52: DiTi Cone Wrench

NOTE: Support the tip adapter by squeezing it


between your fingers when loosening the DiTi
cone.
2. Remove the black tip ejector tube.
3. Disconnect the tubing extension from the tubing.
NOTE: Ensure the O-ring (and separator ring, if
applicable) remains with the tubing extension.
4. Unscrew the stainless steel adapter cylinder.
5. Safely set aside the DiTi cone, tubing extension, O-ring
(and separator ring, if applicable), and adapter cylinder.
6. Screw the reference tip on the tip adapter and tighten.
NOTE: Before mounting the reference tip, check
that the tip is not bent.

Mounting the New Short Reference Tip


The new design allows calibration without removing the
adapter cylinder, tubing extension, O-ring configuration, or
tip ejector tube.

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1. Using the DiTi cone wrench, turn DiTi cone 3 clockwise to


loosen and remove it from the tip ejector tube (See NOTES
under Step 1 above).
2. Remove the DiTi cone only; all other hardware must
remain in place, including the O-ring configuration.
3. Screw the reference tip onto the adapter cylinder until it is
finger tight.

Channel Configuration
1. Select <System Devices><LiHa>.

Figure 4.53: System Devices Menu

2. Select <LiHa>, <Channel> from the <LiHa> menu to


initiate tip configuration.

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3. Configure the tips. Select all eight checkboxes (聺) for


<DiTi> then select <Adapter> for the <Ch.Type> for all
eight tips.

Figure 4.54: <LiHa Panel> Main Menu

4. Select <1000 µL> for the syringe volumes. Select <Apply>


to save the settings.

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Check Each of the Tip Adapters


1. Select the <Contents> page in the LiHa panel and select
the <Tip Adapter> checkbox (聺) under <Test>.

Figure 4.55: <LiHa Panel>, <Contents>

2. Remove all DiTis or reference tips from the Diti adapters.


3. Switch to the <Test Configuration> page.
4. Select all eight tip adapters to test then select <Start>. A
prompt to move the LiHa to a more-accessible location
over the worktable displays.

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5. Manually move the LiHa to the middle-front area of the


worktable then select <OK>.

Figure 4.56: LiHa <Test Configuration>

6. Follow the onscreen prompt and move up the first adapter


to the contact position and hold up in position.

Figure 4.57: Checking Tip Adapter

7. Observe the progress bar on the process prompt to


determine if the tip was detected.

Figure 4.58: Tip Adapter Test

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8. Continue to hold the tip adapter all the way up and


confirm with <Next>.
9. Repeat the test until all tip adapters are checked. The
prompt <Tip Adapter Passed> displays.
10. Select <OK>. The screen reverts to the <Test
Configuration> page.

Determine LiHa Range


This procedure calibrates the Z- and Y-ranges.
1. Select the <Contents> page in the LiHa panel and select
the <Determine Range> checkbox (聺).
Do NOT deselect any previously checked <Setup> or
<Test> or <Alignment> box.
2. Switch to the <Setup> page.
3. Select the <4/8 Reference Tips Available> checkbox (聺).
CAUTION: Do NOT deselect the <Use Y
Backlash> box. Leave it checked! This function
tells the software to accommodate some geared
backlash (hysteresis in the gears).
4. In the <Reference Positions> frame, set the left grid
reference position to 1 and right grid reference position to
39.
5. Select the <Left> option for <Use for Y>.
6. Select <Start>.
7. Follow the onscreen prompts. Z-ranges are determined for
each tip.
8. After the test is complete, do not remove the reference
tips; they are used in the next test.
9. Confirm with Done.

Determine LiHa Reference Position


1. On the <Contents> page select the <Determine
Reference Positions> checkbox (聺) then switch to the
<Setup> page.
Do NOT deselect any previously checked <Setup> or
<Test> or <Alignment> box.

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2. Select the <4/8 Reference Tips Available> checkbox if


eight reference tips are available. If not, deselect the
option.
3. In the <Determine Reference Positions> frame select the
<Manually> option button.
4. Set the <Left> and <Right> grid references to 1 and 39,
respectively.
5. Select <Left> for the grid Y-range grid position.
6. Select <Start> to initiate the procedure.

Figure 4.59: <Setup> Page

7. Follow the onscreen prompts.

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8. Confirm with <Done> when the tips are mounted.


9. The <Move LiHa> page is activated and the message <Use
move tool to determine the first reference point>
displays.

Figure 4.60: <LiHa Panel>, <Move LiHa>

10. Adjust the position in the X- and Y-direction as shown.

Reference Tip

Reference Point

Reference Pin on Worktable

Figure 4.61: Reference Position

11. When the tip is in the correct position, confirm with


<Done>.
12. Repeat alignment for all required positions.
13. After checking all reference positions, the message
<Dismount Reference Tips> displays.

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14. Confirm with <Done>.

Calibrate the Lower DiTi Eject


1. Select <System Devices>, <LiHa> to initiate the
procedure. The <Contents> page displays.
2. Select the <Calibrate Lower DiTi Eject> checkbox (聺).
3. Select <Start>.
4. Enter the serial number of the instrument at the prompt
and select <OK>. The test proceeds automatically and
requires no user intervention.

Figure 4.62: <Contents>

5. At the <LiHa Process Prompt>, select <OK>.


6. Select <Close> again to return to the main menu.

Figure 4.63: <LiHa Process Prompt>

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Perform the Lower DiTi Eject Test


1. Go to <Liquid System>, <DiTi>, <Lower DiTi Eject
Test>.
2. Load the <NAT worktable> or the <m2000sp
Worktable>:
• Place the DiTi rack carrier at grid 21.
• Place the wash/waste station and DiTi slide in the
cutout at grid 18.
• Place a full DiTi rack (1000 µL) on the rear position.
• Place an empty tray on the middle rack
3. Place an empty DiTi tray on a 1000 µL DiTi rack and place
it in the middle position on the carrier. The <Lower DiTi
Eject Test> screen displays.

Figure 4.64: <Lower DiTi Eject Test> Route

4. Configure the Lower DiTi Eject Test as shown:

NAT MBS m2000sp


DiTi Type 1000F EDiTi 1000F EDiTi
Source Rack DiTi.RR C12.RR
First DiTi Position 1 1
Destination Rack DiTi.RM C12.RM
Waste Rack W1.DiTi Waste C11.DiTi Waste

For both setups:


• Leave <Put back DiTi on lower position> blank
• Enter the operator name

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5. Select <Select Tips> and verify all eight DiTis are 1000 µL
DiTis with Filters.

Figure 4.65: <Select Tips>

6. Select <OK>.
7. Select <Run> to initiate the test. The status becomes <Test
Running>.
8. At completion of the test, the status becomes <Test
Passed>.
9. Select <Close> to finish the test.
10. If the test failed:
• Verify the LiHa reference positions.
• Check the tip adapters.
• Perform individual Z alignment.

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• Calibrate the lower DiTi eject.

Figure 4.66: Lower DiTi Eject Test Passed

11. Remove the carrier, DiTi slide, and wash/waste station


from the worktable.

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Y-Spread Procedure

NOTE: The Y-spread tool and ISA 600-015 are required


for this procedure.

1. Select <System Devices>, <LiHa> to initiate the


procedure. The <LiHa Panel> displays.
2. Returning to the main S&S menu, select <Instrument>
then <Information>.
3. Select the <Information> tab and then <LiHa Arm> from
the top field.
4. Select <Axis Parameter> and then find the Ys scaling
factor in the bottom field and write the value down for
later use.

Figure 4.67: Ys Scaling Factor Value

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5. Close the <Information Panel> and select the <Move


LiHa> tab from the <LiHa Panel>.

Figure 4.68: Move LiHa Control

6. Return to the <Instrument> menu and select <Command


Tool>. Select <Single Commands> and enter the
following command in the <Command> field:
A1RPY8

Enter
A1RPY8
here

Figure 4.69: Command Tool Panel

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The value will report as A1@#####,#####. The first


number is the Y scaling; the second number is the Ys
scaling.
7. Run the Y-spread batch file from ISA 600-015 and do the
following:
• Enter the grid location
• Select No when asked if you will be using reference
tips
• Select No when asked if you will be using the front
holes
• Select No when asked if you will be changing the
Y-spread to 18.8 mm
8. Bring tip 3 down to the associated gap on the Y-spread
tool. Center in the X and Y first, and then align in the Z.
Adjust the X and Y if necessary.

Figure 4.70: Tip 3 Aligning with Y-spread Tool

9. Using the <Single Commands> tool, obtain the


parameters (X,Y, and Z) for tip 3 by entering the following
three commands (one at a time) and writing down the
value for Z3:
• A1RPX0
• A1RPY0
• A1RPZ0

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10. Using the <Single Commands> tool again, set all tips to
tip 3’s Z-height on the Y-spread tool by entering the
following command:
A1PAAX,Y,Ys,Z1,Z2,Z3,Z4,Z5,Z6,Z7,Z8
Here X, Y, and Z1-Z8 are the tip 3 values found above, and
Ys is 90 (as set by the Y-spread script).
11. Observe the placement of the tips and adjust the values to
re-center tip 3 if necessary.
Observe the Y-spread at these locations. Ideally, the tips
will not be touching the walls of the Y-spread notches.
If the tips are out of alignment, check for bent tip
adapters and replace if necessary.
If tip 3 or tip 7 are replaced, reperform the LiHa
Determine Range test (refer to Determine LiHa Range on
page 4–60) then perform the Y-spread check again.
12. Using the <Single Commands> tool, reinitialize the axis
by entering the following command:
A1PIZ
13. Run the Y-spread batch file from ISA 600-015 and do the
following:
• Enter the grid location
• Select No when asked if you will be using reference
tips
• Select Yes when asked if you will be using the front
holes
• Select Yes when asked if you will be changing the
Y-spread to 18.8 mm

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14. Align tip 3 with the associated gap on the y-spread tool.

Figure 4.71: Tip 3 Aligning

15. Using the <Single Commands> tool, obtain the


parameters (X,Y, and Z) for tip 3 by entering the following
three commands (one at a time) and writing down the
value for Z3:
A1RPX0
A1RPY0
A1RPZ0
16. Using the <Single Commands> tool again, set all tips to
tip 3’s Z-height on the Y-spread tool by entering the
following command:
A1PAAX,Y,Ys,Z1,Z2,Z3,Z4,Z5,Z6,Z7,Z8
Here X, Y, and Z1-Z8 are the tip 3 values found above, and
Ys is 188 (as set by the Y-spread script).
17. Observe the placement of the tips and adjust the values to
re-center tip 3 if necessary.
Observe the Y-spread at these locations. Ideally, the tips
will not be touching the walls of the Y-spread notches.

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18. Move the tips back and forth over the Y-spread tool from
the front location to the back location.

Figure 4.72: Y-spread Tool

You can use the same commands as were previously


entered by double-clicking on them in the command
window.
19. Observe the Y-spread again at the front location for
scaling. If the tips are set inside the scaling notches, the
scaling factor should be made larger; if the tips are set
outside the notches, the factor should be made smaller.
Using the <Single Commands> tool, change the scaling
factor as needed using the following command:
A1SAS#####
20. Using the <Single Commands> tool, home the Z and Y
axes using the following commands:
A1PIZ (the Z-axis must be homed first)
A1PIY

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21. Move back to the 188 (front) location, then back to the 90
(back) location, then the 188 location again.
90

188

Figure 4.73: Y-spread Tool

Observe the Y-spread in the front location. If satisfied,


move to the back location, and observe the Y-spread
there.
Continue with adjustments until the Y-spread in both
locations is satisfactory.

22. When satisfied, use the <Single Commands> tool to save


the value with the following command:
M1SOW
23. Using the <Single Commands> tool report Y-spread value
with the following command:
A1RPY8
The value will report as A1@#####,#####. The first
number is the Y scaling; the second number is the Ys
scaling.
Write this number down in the event you want to return
to an earlier value.

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Configure the RoMa


RoMa configuration in S&S involves the following activities:
• Performing RoMa setup
• Determining the RoMa reference positions
• Determining the RoMa range
• Auto-adjusting the rotator
• Manually adjusting the rotator

WARNING: Potential Biohazard. Indicates an area


where the FSE or operator may be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator may
be exposed to hazardous chemicals when handling
Abbott Molecular sample preparation reagents.
Exposure to hazardous chemicals is minimized by
following instructions provided in product-specific
labels and product-specific MSDS. Wear appropriate
personal protective equipment, such as gloves, lab coat,
and protective eyewear.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap
• Discharge static electricity from your body before
touching the instrument
CAUTION: Ensure there are no objects installed on the
worktable that could interfere with movement of the
RoMa. It may be necessary to restart the software and
re-enter the password if the software was exited.

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WARNING: Pinch Point. The instrument requires


accurate positioning of system modules, and carriers
and racks on worktables. When working with the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.

Keep body parts away from moving parts of the


instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Keep back from the worktable surface during these
procedures.
• Fast moving devices could cause injuries.

RoMa Move Tool


To perform many of the procedures in this section, it will be
necessary to use the RoMa Move Tool. Refer to Move Pages on
page 11–17 of Section 11 for instructions on using this
feature.

Set up the RoMa


1. Go to <System Devices>, <RoMa>.

Figure 4.74: <System Devices> Menu

2. Switch to the <Printing> page and complete the


<Operator> and <Comment> fields:
• Operator (name)

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• Comment

Figure 4.75: RoMa Panel

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Set Type
1. On the <Contents> page, select <Set Type> then switch to
the <Setup> page.
2. Select:
• <CW> for <Rotator Type>
• <Eccentric> for <Gripper Type>
• <Normal (standard)> for <Z Range>

Figure 4.76: <RoMa Panel>, <Setup> Page

3. Save the selections by selecting <Start>.


4. Confirm the changes with <OK>. The values are written to
the RoMa.

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Determine RoMa Reference Positions


1. Select the <Determine Reference Positions> checkbox
(聺) in the <Contents> page.

Figure 4.77: <RoMa Panel>, <Contents> Page

2. Switch to the <Setup> page.

Figure 4.78: RoMa Panel - Reference Positions Page

3. Define the <Left> reference position for the RoMa as 6 or


7 and the <Right> reference position as 45.
NOTE: The <Left> grid reference position may
either be 6 or 7, depending on manufacturing
tolerances. Check that the reference pin is in the
correct location on the worktable deck (6 or 7).
4. Select the <Left> option for <Use for Y>.
5. Initiate the procedure with <Start>. The message <Mount
Reference Tool> displays.
6. Hold the reference tool against the bottom of the gripper
module and select <OK>. This opens the gripper to hold
the reference tool.

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7. Confirm the message <Ready to Proceed> with <OK>.


The <Move RoMa> page is activated and the message
<Use move tool to determine first reference point>
displays. Grab and drag the <RoMa Process Prompt> box
away from the <Move RoMa> page.
8. Use the X- and Y-arrow keys to move the RoMa to the first
reference point. The Z-arrow keys can be used to lower the
reference tool closer to the reference position.
9. When in the correct position, confirm with <Next>.
10. Repeat for all indicated positions.
11. Press <OK> to release the tool.
12. Press <OK> to proceed.
13. Press <OK> at the prompt <Setup: Done>.
14. If the RoMa is significantly out of alignment or the test
fails, reset the default parameters (Set Default Parameters)
and perform the following:
• Set Type, found on page 4–77
• Determine RoMa Reference Positions, found on page
4–78
• Determine Range, found on page 4–81
• Adjust the Rotator, found on page 4–82

Reference Plate Test


NOTE: Ensure that 3M foam tape is installed on the
reference plate prior to beginning this procedure, as
required in the TSB.
1. From the <Contents> page select the <Reference Plate
Test> checkbox (聺).
2. Switch to the <Test Configuration> page and select grid
25 for the Reference Plate Position grid, and 5 cycles.
3. Select <Start>.
4. At the prompt, mount the gripper fingers then press <OK>
to continue.
5. Place the reference plate at grid position 25.
6. Select <OK> to initiate the procedure.

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7. The RoMa attempts to pick up the reference plate.


Determine if the reference plate is picked up squarely with
equal pressure applied from both fingers.
8. If no adjustment is necessary, press <OK>, close the RoMa
panel, and proceed to the first PosID test.
9. If adjustment is required, perform Adjust the Rotator,
found on page 4–82.

Set Default Parameters (As Needed)


1. On the <Contents> page, select <Set Default Parameters>
and select <Start>. The ranges, reference positions, and
rotation ranges were reset to the default values and must
be reestablished.

Figure 4.79: RoMa <Contents> Page

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Determine Range
1. On the <Contents> page, select the <Determine Range>
checkbox (聺) and switch to the <Setup> page.

Figure 4.80: RoMa <Contents> Page

2. In the <Reference Positions> frame. Select <Left> for the


<Use for Y> reference position, and set the left <Grid>
spin box to 6 or 7 and the right <Grid> spin box to 45.

Figure 4.81: RoMa <Reference Positions>

NOTE: The left grid reference position may be


either 6 or 7, depending on manufacturing
tolerances. This has no impact on the range or
accuracy of the RoMa.
3. Select <Start> to initiate the procedure. The RoMa moves
and determines the range. The message <Mount
Reference Tool> displays.

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4. Hold the reference tool against the gripper module and


select <OK>. This opens the grippers to hold the reference
tool.
5. Confirm the message <Ready to Proceed> with <OK>.
After determining the range, the message <Unmount
Reference Tool> displays.
6. To prevent the reference tool from falling, hold the
reference tool in the gripper then select <OK>. The
reference tool is released and values are saved. Select <OK>
at the prompt.
7. Select <OK> again at the <Setup: Done> prompt.

Adjust the Rotator


NOTE: If the gripper fingers were removed, mount the
gripper fingers. Use the RoMa move option to move the
RoMa to a position where the grippers can be mounted
easily. Once they are mounted, lower the gripper head
down to a flat surface (such as the 1 mL Subsystem) and
adjust the fingers so that they contact the worktable at
the same time. When finished, raise the gripper head to
its initialized position.
NOTE: Ensure that 3M foam tape is installed on the
reference plate prior to beginning this procedure, as
required in the TSB.

1. On the <Contents> page, select the <Adjust Rotator>


checkbox (聺) and switch to the <Setup> page.

Figure 4.82: RoMa <Contents> Page

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2. Select 25 to define the grid position of the test plate (front


left corner).

Figure 4.83: RoMa <Setup> Page

3. Select <Start> to initiate the procedure. The message


<Mount Gripper> displays.
4. The message <Make sure the reference plate is on Grid
25> displays.
5. Put the test plate on the worktable at the selected grid
position (front-left corner) and continue with <OK>. The
rotator is adjusted and the values are saved. Press <OK>
when the test is complete.
6. Verify the setup by performing the <Reference Plate> test.
7. Select <Close> to exit the RoMa panel.

Manual RoMa Rotator Adjustments


Both rotational offsets in the firmware and mechanical offsets
in the hardware are included in this adjustment as needed.
1. Check the parallelism of the arms by placing the reference
plate on the deck.
Use the RoMa move tool to position the RoMa arms
directly over the plate, with the grippers open to the
width of the plate.

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Figure 4.84: RoMa Positioned Over the Plate

2. Observe the arm position in relation to the plate. They


should run parallel.
If the arms are not parallel, it might be necessary to bend
them slightly.
3. Rerun the Reference Plate Test, found on page 4–79.
If the plate is gripped and replaced with a minimum of
slippage from the pins to the deck, leave them as is and
move on with the calibrations. This instruction applies to
tests that are borderline but still passing, as well.
NOTE: If calibrations cannot be passed with the
rotation as is, return to S&S and follow the
rotation adjustments outlined below.
If the test fails, manually fix the rotation as instructed in
Step 4 below.

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4. Remove the gripper arms from the RoMa head. Use the
<RoMa Move> screen to align the front line of the gripper
mount to the back of the 1 mL Subsystem or the back of
an MTP carrier (the important thing is that it is aligned to
a straight edge).

Figure 4.85: RoMa Head Aligned with 1mL Subsystem

5. Use the <Single Commands> tool to report the rotator


value with the following command:
C1RPR4
Record this reported value prior to making any changes.
6. Use the <Single Commands> tool to change the rotator
value with the following command:
C1SOR###
7. Use the <Single Commands> tool to reinitialize the
rotator with the following command:
C1PIR
8. Observe the rotator head to see if the front of the gripper
mount is parallel with the straight edge of the 1 mL
Subsytem.
Repeat the above steps until the desired parallelism is
reached, then remount the gripper arms.
9. Rerun the Reference Plate Test. If the plate is gripped and
replaced satisfactorily, save the value to memory using the
following single line command before continuing with
calibrations:
C1AWE

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Configuring Sample Bar Code Labels


The Configuring Sample Bar Code Labels procedure is
performed within the m2000sp software. Refer to Sample Bar
Code on page 4–182.

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Configure the PosID 2


PosID 2 configuration in S&S involves the following activities:
• Performing the PosID functionality test
• Confirming basic mechanical accuracy
• Performing PosID 2 setup

PosID Functionality Test


WARNING: Class II Laser Product. This Class 2 laser
product contains an embedded low power visible Class
2 laser diode bar code scanner. Avoid direct viewing of
the bar code laser beam or reflections of the beam.
NOTE: The bar codes for the Output Deck and
GPReagent Rack are not read by this test and will not
appear in the results.
NOTE: If you have loaded the <NAT MBS Worktable>,
you must run the PosID tests using 100 mL reagent
carriers and troughs. If you have loaded the <m2000sp
Worktable>, you must run the PosID tests using 200
mL reagent carriers and troughs.
1. Select <System Devices>, <PosID>, <Test>. The <PosID2
Test Configuration> dialog displays.

Figure 4.86: PosID2 Test Configuration Dialog

2. Set <Number of cycles:> to 5.


3. Set <Grid-Positions> from 1 to 40.

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4. Select the <Read Carrier> checkbox (聺) to read carriers


and containers.
5. Select the <Read User ID> checkbox (聺) only if user
identifications are used.
6. Enter operator name and instrument serial number.
7. Load the worktable with bar-coded carriers. Refer to the
m2000sp Operations Manual for instructions on labeling
carriers, racks, and/or tubes with bar code labels.
If the <m2000sp Worktable> is loaded, run the test with
the following:
• Two carriers, at grids 4 and 5, with multiple bar-
coded containers (sample tubes). The bar codes
should face the slots on the right side of the tube
carriers.
• One 200 mL reagent carrier with reagents at grid
19-20.
• Two DiTi carriers with left edges at grids 21 and 27.
• Chilled Output Deck at grids 35-40.
If the <NAT MBS Worktable>, is loaded, run the test with
the following:
• Two carriers, at grids 4 and 5, with multiple bar-
coded containers (sample tubes). The bar codes
should face the slots on the right side of the tube
carriers.
• One 100 mL reagent carrier with reagents at grid
19.
• Two DiTi carriers with left edges at grids 21 and 27.
• Two sample output carriers at grids 38 and 39.
8. Start the test procedure with <Run>. An empty <PosID 2
Test Results> window opens.
9. Begin the test procedure with <Start>.
10. The instrument initializes and the PosID starts to check
the carriers and containers.
11. Values in the <PosID 2 Test Results> window are
continuously updated during the test.

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12. Select the <Show Container Reads> option at the bottom


of the screen to show both carrier and container read data
during the test.

Figure 4.87: PosID 2 Test Results Window

13. The following data can be read in the window:


• Heading line displays the number of reads
done/defined and worktable position, for which
results display in the table. The position can be
switched using the scroll bar.
• <NT> shows the reading result of the No Tube sensor
during the Reference Read. Two letters can display:
– <T>: Tube found
– <N>: No tube found
• <Reference Read> indicates which bar codes were
read in the reference read. Two sets of characters can
display:
– <***> indicates no reading possible, but tube is
placed correctly
– <$$$> indicates no tube found in this position
• <NT> shows the reading result of the No Tube sensor
during the Actual Reads.
• <Actual Read> indicates bar codes which were read in
the Actual Reads.

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• <NT> stands for “No Tube” and indicates no tube


found in the read attempt.
• <NR> stands for “No Read” and indicates no bar codes
were read, but tubes were placed correctly.
• <WR> stands for “Wrong Read” and indicates bar
codes were read that do not match bar codes read in
the Reference Read.
• <Total Errors> indicates the total errors that occurred
during a test run.
14. The message <PosID 2 Test passed> or <PosID 2 Test
failed> displays.
15. Confirm with <OK>.
16. Select <Close> to close the window. The <PosID 2 Test
Configuration> dialog is active again.
17. Print the test results with <Print> and/or finish the
function with <Close>.
18. The test passed if the following criteria are met:
• <No Tube>: zero errors
• <No Read>: not more than five errors
• <Wrong Read>: zero errors
No other setup of the PosID 2 is required.
19. If the test failed, verify correct positioning of the bar code
labels and bar code types, and/or setup of the PosID as
described in Set Up PosID 2, found on page 4–92.

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Verify Basic Mechanical Accuracy

NOTE: The PosID Service Rack is required for setup


functions.

Figure 4.88: PosID Service Rack

1. Switch off the instrument.


2. Use a feeler gauge to check the gap between the release
finger of the PosID 2 and worktable surface. Ensure the gap
is approximately 0.2 mm.

Figure 4.89: Release Finger Adjustment

3. If necessary, loosen the two fixing screws and adjust the


release finger then tighten the screws.
4. Place the Service Rack onto a grid position on the
worktable.
5. Manually move the PosID 2 gripper into the Service Rack.

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6. Check the gap between the Service Rack and gripper pin.
If needed, adjust to approximately 0.5 mm.

Figure 4.90: Mechanical Adjustment

7. Switch on the instrument and initialize with <System>,


<Instrument>.

Set Up PosID 2
1. Start the function with <System Devices>, <PosID>,
<Setup>.

Figure 4.91: System Devices Menu <PosID>, <Setup>

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2. Select <Set>. The PosID 2 moves through all five logical


positions and the gripper moves through all eight possible
logical axes.

Figure 4.92: PosID 2 Adjustment Dialog

3. Select <Run>. The PosID 2 runs in all axes to the end


positions to determine its range.
4. Set the <Left Position> to 1, <Rack Position> to 10, and
<Right Position> to 41.
5. Select <Start> to start setup. A prompt displays to insert
the test rack at the defined position.

Figure 4.93: Insert Test Rack Message

6. Slide the Service Rack into grid 1. Do not push it to its stop
position. Verify it is approximately 1 cm in front of the
stop position.

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7. Confirm with <OK>. The <Move PosID: Move Gripper to


Position 1> dialog displays.

Figure 4.94: <Move Gripper to Position 1> Dialog

8. Carefully push the Service Rack toward the rear of the


instrument to its stop position.
9. Check the position of the gripper pin against the adjusting
slot of the Service Rack. The pin must fit exactly in the slot.

Figure 4.95: Adjusting Slot Position

10. If the position is incorrect, align it using the movement


buttons.
11. When the gripper pin fits in the slot, confirm the
adjustment with <OK>. The message <Please insert Test
Rack in Position 41> displays.

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12. Slide the Service Rack into grid 41. Do not push it to its
stop position. Verify it is approximately 1 cm in front of
the stop position.
13. Confirm with <OK>. The <Move PosID: Move Gripper to
position 41> dialog displays.
14. Carefully push the Service Rack toward the rear of the
instrument to its stop position.
15. Check the position of the gripper pin against the adjusting
slot of the Service Rack. The pin must fit exactly in the slot.

Figure 4.96: PosID Service Rack

16. If the position is incorrect, align it with the movement


buttons.
17. When the gripper pin fits in the slot, confirm the
adjustment with <OK>. The message <Please Insert Test
Rack in Position 10> displays.
18. Slide the Service Rack into grid 10 to its stop position.

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19. Confirm with <OK>. The mechanical adjustment starts


when the <Move PosID: Mechanically adjust beam
angle vertical> dialog displays.

Figure 4.97: <Mechanically Adjust Beam Angle Vertical>

Scanner Head Mechanical Adjustment


1. The laser beam must be parallel to the vertical guideline
on the Service Rack.

Carrier ID Read Position

Guideline
Laser Beam

Correct Incorrect

Figure 4.98: Carrier PosID

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2. If the beam is not parallel, loosen one of the two fixing


screws for the horizontal adjustment with a 2.5 mm Allen
key and adjust the scanner head accordingly. Tighten both
screws when adjustment is complete.

Fixing Screws for Horizontal


Adjustment (Step x)

Fixing Screws for Horizontal and


Vertical Adjustment (Step x)

Fixing Screw (Vertical Adjustment)


(Step x)

Fixing Screw (Vertical Adjustment)


(Step x)

Figure 4.99: Scanner Head Adjustment Screws

3. Adjust the X-positioning until the laser beam and


guideline are superimposed.

Service Rack

Horizontal Calibration
Line

Vertical Calibration Line

Laser Beam

Correct Incorrect
Figure 4.100:Vertical/Horizontal Scanner Head Adjustment

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4. Confirm with <OK>. The gripper finger moves the Service


Rack to its center position. The <Move PosID:
Mechanically adjust head vertical> dialog displays. The
laser beam must be parallel to the vertical calibration line
on the Service Rack.

Figure 4.101:<Mechanically Adjust Head Vertical>

5. If the beam is not parallel, loosen one of the two fixing


screws for horizontal and vertical adjustment with an
Allen key and adjust the scanner head accordingly.
Tighten both screws when adjustment is complete.
6. Use the arrow keys to move the PosID 2 until the laser
beam and vertical calibration lines are superimposed.

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7. Confirm with <OK>. The <Move PosID: Mechanically


adjust beam height> dialog displays.

Figure 4.102:<Mechanically Adjust Beam Height> Dialog

8. The laser beam must be parallel and flush to the horizontal


calibration line on the Service Rack.
9. Perform the parallel adjustment using the movement
buttons. Movement must always be done from the CW
direction because of hysteresis. First, move the scanner
head back with the <CCW> key, then move the scanner
back into the correct position with the <CW> key until the
beam is superimposed with the calibration line.
10. If the beam is not in line with the horizontal calibration
line, insert an Allen key through the access hole and move
the scanner head until the beam is flush with the
horizontal calibration line.

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11. Confirm with <OK>. The mechanical adjustment is


executed and the position of the bar code reader is stored.
The <Move PosID: Adjust carrier reading position>
dialog displays. The laser beam must be parallel and flush
with the vertical guideline on the Service Rack.

Figure 4.103:<Adjust Carrier Reading Position> Dialog

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12. Confirm with <OK>. The <Move PosID: Adjust change


position> dialog displays.

Figure 4.104:<Adjust Change Position> Dialog

NOTE: The change position is the transition


position in which the scanner head changes from
moving around its vertical B-axis to moving
around its horizontal X-axis, or vice versa. Since
mechanical movement always has hysteresis, the
adjustment procedure must be started from the
same direction.
NOTE: Always rotate the scanner head with
<CCW> back to the correct position to avoid
potential mechanical problems (collisions).
13. With the Beam Angle (deg) List, set the step size to 1.0.
14. Rotate the scanner head counterclockwise with <CCW>
until it rotates, to verify it is not already in the change
position.
15. Rotate the scanner head clockwise with <CW> until it
stops rotating in the vertical B-axis.
16. Count the number of steps necessary until the scanner
head starts to rotate into the horizontal X-axis.
17. Go back counterclockwise, half the number of steps
counted plus two additional steps.
NOTE: These two additional steps are necessary to
compensate for space in the mechanical system.

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18. Go two steps clockwise, to arrive exactly at the change


position.
19. Confirm with <OK>.
20. The PosID starts with the No Tube Sensor test. The gripper
finger slowly moves the Service Rack backward in the
Y-direction until the No Tube sensor passes all three slots
in the Service Rack.
21. If the No Tube Sensor test passed, the following four
checkboxes are selected (聺) in the <PosID 2 Adjustment>
dialog.
• Slot begin
• Slot end
• Check sensor upper limit
• Check sensor lower limit
22. If the No Tube sensor test failed, the No Tube sensor is
damaged and must be replaced. Refer to No Tube Sensor
on page 9–172 of Section 9, Repair, Replacement of Spare
Parts.
23. The setup procedure continues. The gripper finger moves
the Service Rack to its center position and the <Move
PosID: Adjust tube reading position> dialog displays.
The laser beam must be in line with the vertical calibration
line of the Service Rack.

Figure 4.105:<Adjust Tube Reading Position> Dialog

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24. If the laser is out of line, use the rotation buttons to adjust
the scanner head accordingly.
25. Confirm with <OK>. The tests for <Adjust scan line start>
and <Adjust scan line stop> are performed automatically.
PosID 2 adjustment was completed successfully.

Figure 4.106:PosID 2 Adjustment Dialog

26. After passing the setup procedure successfully, the


<PosID 2 Adjustment> dialog displays again. Except for
the <Adjust failed> checkbox, the checkboxes are checked
(聺) to indicate all appropriate tests were completed.

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27. When the <PosId 2 adjust: Adjust passed> dialog


appears, select <OK>.

Figure 4.107:<Adjust Passed> Message

28. If the setup procedure failed, the <Adjust failed>


checkbox is checked (聺), and the setup procedure must be
repeated.

Installation of Wash/Waste Station


1. Install the wash/waste station on the worktable surface at
grid 18.

Figure 4.108:Wash/Waste Station on Worktable

2. Secure to the surface with the included screw.


3. Install two pieces of short tubing to the two nipples of the
wash/waste station that extend below the worktable
surface into the cabinet (as shown in Figure 4.109 below).
Secure with tubing clamps.

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Figure 4.109:Underside of Wash/Waste Station

Ensure the waste station chute is securely positioned and


that the ramped section at the bottom of the chute points
to the left, toward the center of the solid waste container.

A warning sticker
shows when the
waste chute is
being installed
incorrectly.

A green circle
appears on the
front of the waste
chute.

Incorrect Figure 4.110: Waste Chute Properly Positioned


positioning of
the waste IMPORTANT: If the waste chute’s red warning
chute could sticker is visible after it is reinstalled, it has been
result in errors positioned incorrectly. The green circle label
must face the user.

Calibrate and Verify System Liquid, Liquid Waste, and Solid Waste Sensors
Adjustments and calibrations are performed through the
Genesis S&S software.
NOTE: To access S&S, log on to the SCC and select
<System>, <Setup and Service>.

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Calibration of DiTi Waste Sensor


1. Go to the <Options>, <SMIO for NAT> menu.

Figure 4.111: Options Menu

2. Select <DiTi Waste Sensor Calibration> under <Setup>.

Figure 4.112: SMIO Panel Menu

3. Go to the <Tools & Configuration> tab.

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4. Verify the <Device Type> is <NAT>. (If not, select <NAT>


from the pull-down menu.) Then select <Diti Waste Full>.
Clear the other checkboxes.

Figure 4.113: Tools & Configuration Tab

5. Select <Start>. The prompt <Place a reflecting plate at


about 120 [mm] below the ultrasonic sensor> displays.
Instead of placing the plate at 120 mm, place the plate at
300 mm below the sensor.

Figure 4.114: Process Prompt: Reflector Plate

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6. Select <Next>. The prompt <Turn both potentiometers


counterclockwise to their stop positions> displays.
NOTE: The potentiometers are made of plastic. Be
careful not to damage them by applying too much
force.

Figure 4.115: Process Prompt: Potentiometers

7. Select <Next>. The prompt <Adjust the distance


potentiometer (top potentiometer) to the point where
the integrated LED just lights up> displays.

Figure 4.116: Process Prompt: Adjust Potentiometers

8. Select <Next>. The prompt <Setup: Done> displays.

Figure 4.117: Process Prompt: Setup

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9. Select OK.
NOTE: If the calibration fails, check the
connection to the SMIO board and repeat the test.
NOTE: If the calibration fails again, replace the
sensor.
10. Perform the DiTi Waste Sensor Verification on page 4–114.

Adjustment of System Liquid Level Sensor


Potentiometers
NOTE: System liquid level sensing is accomplished by
changes in capacitance. Water droplets on the inside
and outside of the liquid container can adversely affect
potentiometer adjustment and sensor calibration.
1. Remove the two screws securing the sensor to the bracket.
2. Pour 1 L of water into the container and allow the water to
settle.
3. Move the sensor to the left to access the potentiometer
adjustment screw located on the rear face of the sensor.
NOTE: The potentiometers are made of plastic. Be
careful not to damage them by applying too much
force.
4. Keep the sensor at the same level as the mounting
brackets.
5. Hold the sensor in place with one hand or use the
container to hold the sensor in place against the bracket.
6. With the container in place, adjust the potentiometer so
the sensor LED is on.
7. Adjust the potentiometer in the opposite direction until
the LED just goes off.
8. Add 1 L of liquid to the container, for a total of 2 L in the
container.
9. Verify the sensor LED is on.
10. Attach the sensor to the bracket and perform the System
Liquid Bottle Sensor Verification on page 4–111.

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Waste Liquid Sensor Potentiometer Adjustment


NOTE: The waste liquid sensor is an optical sensor.

1. Disconnect the waste container hoses and remove the cap.


2. Pour 8.5 L of water into the container and allow the water
to settle.
3. Switch the instrument on.
4. Adjust the potentiometer, located on the end of the
sensor, counter clockwise until the sensor LED goes out.
NOTE: The potentiometers are made of plastic. Be
careful not to damage them by applying too much
force.
5. Turn the potentiometer clockwise slowly until sensor LED
turns on.
6. Turn the potentiometer clockwise another 180 degrees
(half turn).
7. Add 1.5 L of liquid to the container, for a total of 10.0 L in
the container.
8. Verify the sensor LED is off.
9. Pour out the liquid. Dry the outside surface of the
container, but leave the inside wet.
10. Place the container back in position and check to ensure
the sensor light is on.
11. If the sensor is off perform a minor adjustment of the
potentiometer until the sensor is on.
12. If necessary, repeat steps 1 through 11 to verify accuracy
of the potentiometer adjustment.
13. Perform the Liquid Waste Bottle Sensor Verification on
page 4–112.
14. Replace the sensor only after two (2) consecutive failed
adjustment attempts.

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Sensor Verification

System Liquid Bottle Sensor Verification


1. From S&S, go to the <Options>, <SMIO for NAT>. The
SMIO Panel screen displays.

Figure 4.118: SMIO Panel Screen

2. Select the <Sensors> test box.


3. Go to the <Tools & Configuration> tab and check the
<System Bottle Empty> box, ensuring that no other
boxes are checked.
4. Select <Start>. The prompt <Fill system bottle with 1.0
[liter] of liquid> displays.

Figure 4.119: Process Prompt: 1.0 Liter

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5. Select <Next>. The prompt <Add 2.0 [liter] of liquid>


displays.

Figure 4.120: Process Prompt: 2.0 Liter

6. Select <Next>. If the test was successful, the prompt


<Sensor Test: Passed> displays.

Figure 4.121: Test Passed

7. If the test fails, adjust the system liquid sensor


potentiometer in one of the two ways described in the
previous section until a <Pass> screen is obtained. Select
<OK> to complete the verification.

Liquid Waste Bottle Sensor Verification


1. From S&S, go to <Options>, <SMIO for NAT>.
2. Select the <Sensors> test box.
3. Go to the <Tools & Configuration> tab and check the
<Wash Bottle Full> box, ensuring no other boxes are
checked.

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4. Select <Start>. The prompt <Fill waste bottle with 8.5


[liter] of liquid> displays.

Figure 4.122: Process Prompt: 8.5 Liters

5. Select <Next>. The prompt <Add 2.0 [liter] of liquid>


displays.

Figure 4.123: Process Prompt: 2 Liters

6. Select <Next>. If the test was successful, the prompt


<Sensor Test: Passed> displays. Select <OK> to finish the
verification.

Figure 4.124: Test Passed

7. If the test fails, adjust the potentiometer as described in


Waste Liquid Sensor Potentiometer Adjustment on page
4–110 until a <Pass> screen is obtained. Select <OK> to
complete the verification.

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DiTi Waste Sensor Verification


1. From S&S, go to <Options>, <SMIO for NAT>.
2. Select the <Sensors> test box.
3. Go to the <Tools & Configuration> tab and check the
<DiTi Waste Full> box, ensuring that no other boxes are
checked.
4. Select <Start>. The prompt <Place a reflecting plate at
180 [mm] below the sensor> displays. Instead of placing
the plate at 180 mm, place the plate at 360 mm below the
sensor.

Figure 4.125: Process Prompt: Reflecting Plate

5. Select <Next>. The prompt <Place the plate at 120 [mm]


below the sensor> displays. Instead of placing the plate at
120 mm, place the plate at 300 mm below the sensor.

Figure 4.126: Process Prompt: 120 mm

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6. Select <Next>. If the test was successful, the prompt


<Sensor Test: Passed> displays.

Figure 4.127:Test Passed

Reinstallation of the Output Deck


Return the Output Deck to the worktable and reconnect all
cables per the latest revision of TSB 605-003.

Perform Peltier Chiller Temperature Check


Verify the chiller is on, then refer to Chiller and Heater
Temperature Check, found on page 5–52 of Section 5,
Maintenance and Diagnostic Procedures.

Check Door Locks


1. From S&S, go to <Options>, <Access Status Options>.

Figure 4.128: <Access Status Options> Route

2. A prompt to enter the instrument serial number may


display. Enter the serial number then select <SET>.
3. The <Access/Status Panel window with activated
<Contents> page displays.

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4. In the <Available Devices> section, select <Access


Status> to test the door locks.

Figure 4.129: <Access / Status Panel>

5. Select the <Device Test> under <Test>.


6. Change to the <Configuration & Tools> page.
7. Select:
• Door Lock 1 (left)
• Door Lock 2 (right)

Figure 4.130: Door Locks 1 & 2

8. Select <Apply> at the lower-right corner to save the


selected configuration.
9. Select <Start> to initiate the protocol.

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10. The prompt <Door Lock 1 (left) / Please close the main
door> displays. Select <OK>.

Figure 4.131: Close Door Prompt

11. The prompt <Door Lock 1 (left) / Check if the left side
is now locked> displays. Select <OK> if successful; if
unsuccessful, select <Cancel>.
NOTE: If <Cancel> is selected because the lock is
not functioning, troubleshoot the lock.

Figure 4.132: Check Door Prompt

12. The prompt <Door Lock 1 (left) / Check if it is unlocked


now> displays. Select <OK> if successful; if unsuccessful,
select <Cancel>.
NOTE: If Cancel is selected because the lock is not
functioning, troubleshoot the lock.

Figure 4.133: Check Door Is Unlocked

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13. The prompt <Door Lock 2 (right) / Please close the main
door> displays. Select <OK>.

Figure 4.134: Close Door Prompt

14. The prompt <Door Lock 2 (right) / Check if the right


side is now locked> displays. Select <OK> if successful; if
unsuccessful, select <Cancel>.
NOTE: If Cancel is selected because the lock is not
functioning, troubleshoot the lock.

Figure 4.135: Check Door Prompt

15. The prompt <Door Lock 2 (right) / Check if it is


unlocked now> displays. Select <OK> if successful; if
unsuccessful, select <Cancel>.
NOTE: If Cancel is selected because the lock is not
functioning, troubleshoot the lock.

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16. The prompt <Test Result: Passed / Access/Status Test:


Passed> displays.

Figure 4.136: Test Passed

17. Select OK.


18. Select OK again to exit.

Verify Temperature Performance of 1 mL Subsystem Heater Blocks


Perform the 1 mL Subsystem Heater Block Temperature Check,
as follows:

Access the Command Tool


1. Go to <System>, <Setup and Service>, then go to
<Options>, <SMIO for NAT>.
2. Open the Single Line Command Tool protocol by
selecting <Command Tool>, <Single Commands>.
3. The <Single Commands> page displays.
4. Set the <Timeout> value to <20000>.

Check Heater Zone 1


1. Type <O4STT500> in the <Command> line and select
<Transmit> to turn on heater zone 1. Set the temperature
to 50.0°C.
NOTE: Turn both heaters on at the same time to
consolidate warm-up time.
2. The <Answer> should be <O4@> to indicate the heater is
on.
3. Wait 20 minutes for the heater block to equilibrate.
4. Type <O4RAT> in the <Command> line and select
<Transmit> to read the actual temperature.

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5. The <Answer> displayed is in the format <O4@XYZ>,


where XYZ is the temperature (in °C) times 10. The value
of XYZ for heater zone 1 should be 475 to 525 (47.5 to
52.5°C).
6. Turn off the heater after completion of the check by
entering <O4STT0> in the <Command> line or cycling
power to the analyzer.

Check Heater Zone 2


1. Type O5STT750 in the <Command> line and select
<Transmit> to turn on heater zone 2. Set the temperature
to 75.0°C.
2. The <Answer> should be <O5@> to indicate the heater is
on.
3. Wait 40 minutes for the heater block to equilibrate.
4. Type <O5RAT> in the <Command> line and select
<Transmit> to read the actual temperature.
5. The <Answer> displayed is in the format <O5@XYZ>,
where XYZ is the temperature (in °C) times 10. The value
of XYZ for heater zone 2 should be 725 to 775 (72.5 to
77.5°C).
6. Turn off the heater after completion of the check by
entering <O5STT0> in the <Command> line or cycling
power to the analyzer.

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Section 4 Set Up Liquid System

Set Up Liquid System


Liquid system setup involves the following activities:
• Setting up the FaWa
• Performing the fill/flush protocol
• Performing the FaWa Pump Test
• Performing the Liquid Detection Test

FaWa Pump Setup


1. Start the function with <Liquid System>, <FaWa>,
<Setup>. The <Fast Wash Pump Setup> dialog displays.

Figure 4.137:<Liquid System> Menu

2. Select 1 for the <Number of Pumps>.


3. Select 8 for the <Number of Tips for each Pump>.

Figure 4.138:<Fast Wash Pump Setup> Dialog

4. Confirm the entries with <OK>.


5. If any data were changed, the message <Write results to
MPO EEPROM> displays. Confirm with <Yes>.

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FaWa Fill/Flush Protocol


1. Load either the <NAT MBS Worktable> or the <m2000sp
Worktable>.
NOTE: If you have loaded the <NAT MBS
Worktable>, you must run the Liquid System tests
using 100 mL reagent carriers and troughs. If you
have loaded the <m2000sp Worktable>, you must
run the tests using 200 mL reagent carriers and
troughs.
2. Select <Liquid System>, <FaWa>, <Test> from the menu.
The <Fast Wash Pump Test Configuration> screen
displays.

Figure 4.139:<Fast Wash Pump Test Configuration>

3. Set <DiTi Type> to 1000F EDiTi.


4. Select <Fill/Flush>. The <Fill Liquid System> screen
displays.

Figure 4.140:<Fill Liquid System>

5. Select the <Use Fast Wash Pump> checkbox (聺).

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6. Select <W1.WASTE,RM> from the pulldown menu for the


<Flush Station> and <W1.DiTiWaste> for the <DiTi
Waste Rack>.
7. Set the <System Liquid Aspirate Speed> to <1000 µL/s>.
8. Set the <Fill Speed> to <1000 µL/s>.
9. Set the <Fill Liquid Volume> to <30000 µL>.
10. Select <Fill System> to initiate filling of the dispense
system.

Elimination of Bubbles
1. To use the above FaWa Fill/Flush Protocol to eliminate
bubbles from the dispense tubing and syringes, deselect
the <Use Fast Wash Pump> checkbox (□), reduce the
<Fill Liquid Volume> from 30000 to 3000 and select <Fill
System>.
2. While the test is running, tap the liquid lines to dislodge
any bubbles lodged in the dispense tubing network.

Perform FaWa Pump Test


NOTE: Use only 13 mm diameter tubes with a
minimum length of 100 mm.
• Ensure test tubes are empty and clean.
• Ensure the worktable map was loaded.
NOTE: If you have loaded the <NAT MBS
Worktable>, you must run the Liquid System tests
using 100 mL reagent carriers and troughs. If you
have loaded the <m2000sp Worktable>, you must
run the tests using 200 mL reagent carriers and
troughs.

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1. Start the function with <Liquid System>, <FaWa>,


<Test>. The <Fast Wash Pump Test Configuration>
dialog displays.

Figure 4.141:<Liquid System> Menu

2. Set the appropriate test configuration:


• <Prefill Volume>: <600 µL>
• <Destination Rack>: <C4.R1> (strip rack with eight
clean 13 mm tubes in the first eight positions)
NOTE: If 16 mm tubes must be used, place the
racks in grids C1-C3.R1, as needed.
• <Flush Rack>: <W1.WASTE,RM>
• <DiTi Type>: <1000F EDiTi>
• <DiTi Rack> (full): <DiTi.RR> (carrier at grid 21)
• <DiTi Waste Rack>: <W1.DiTiWaste>
• <First DiTi Position>: <1>

Figure 4.142:<Fast Wash Pump Test Configuration> Dialog

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3. Select <More>. The <Fast Wash Pump Test-Adv.


Configuration> page displays.

Figure 4.143:<Fast Wash Pump Test - Adv. Configuration>

4. Set the advanced configuration:


• <User defined>: selected
• <Name of user defined Test>: <FaWa>
• <Duration>: <30>
• <Minimal Throughput to pass Test>: <1600>
5. Select <OK>.
6. The prompt <Your user-defined configuration is
accepted. FaWa-Test will run.> displays. Select <OK>.

Figure 4.144:FaWa Test Prompt

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7. Start the test with <Run>. The <Fast Wash Pump Test
Results> window opens.

Figure 4.145:FaWa Test Results Screen

8. Begin the test procedure with <Start>. The test starts


running.
9. After finishing the test, the message <Fast Wash pump
test passed> or <Fast Wash pump test failed> displays.
Confirm with <OK>. The <Fast Wash Pump Test Results>
window with final test results becomes active.

Figure 4.146:<Fast Wash Pump Test Results> Window

10. Close the window with <Close>. The <Fast Wash Pump
Test Configuration> dialog is active again.
11. Finish with <Cancel>.
12. The test passed if the throughput value is greater than the
minimal throughput value displayed in the <Tip>
column. If the value under <Measured> is less than the
expected throughput value, the test failed.
13. If the test failed, verify tips are not clogged and recheck the
liquid system for air bubbles.

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Section 4 Set Up Liquid System

Perform Liquid Detection


NOTE: Tubing should be bubble free.

NOTE: Use only 13 mm diameter tubes with a


minimum length of 75 mm.

1. Load the NAT or m2000sp worktable map with <System>,


<Load Worktable>.
NOTE: If you have loaded the <NAT MBS
Worktable>, you must run the Liquid System tests
using 100 mL reagent carriers and troughs. If you
have loaded the <m2000sp Worktable>, you must
run the tests using 200 mL reagent carriers and
troughs.
2. Fill a clean reagent trough with 40 to 60 mL of tap water.
Place the trough into the front portion (RF) of a reagent
carrier and load the carrier on grid 20 of the worktable.
3. Place a full rack of 1000 µL DiTis onto the rear position
(RR) of a DiTi carrier and place the carrier on grid 21 of the
worktable.
4. Place a rack with eight tubes in positions 1 through 8 on
grid 1 of the worktable.
5. Select <Liquid System>, <Liquid Detection>, <Setup>,
then select:
• <Use Trough Detection Mode>
• <Save>
• <OK>
• <Close>

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6. Begin the function with <Liquid System>, <Liquid


Detection>, <Normal Test>. The <Liquid Detector Test
Configuration> dialog displays.

Figure 4.147:<Liquid Detection> Submenu

7. Set the appropriate test configuration according to the


worktable map.
• <Cycles>: 15
• <Submerge>: 10
• Select <Is the Liquid Source a Rack?>
• <Source Rack>: <T1.RF>
• <Flush Rack>: <W1.WASTE,RM>
• <Destination Rack>: <C4.R1>
• <First Dest. Position>: <1>
• <DiTi Type>: <1000F EDiti>
• <DiTi Rack>: <DiTi.RR>
• <DiTi Waste Rack>: <W1.DiTiWaste>
• <First DiTi Position>: <1>

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Figure 4.148:<Liquid Detector Test Configuration> Dialog

8. Start the test with <Run>. An empty <Liquid Detector


Test Results> window opens.

Figure 4.149:<Liquid Detector Test Results>

9. Begin the test procedure with <Start>. The message <Do


flush step?> displays.

Figure 4.150:<Do Flush Step?> Message

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10. Confirm with <Yes>. Select <No> when <Repeat flush


step?> displays a second time. The test starts running.
11. During the test, results display continuously on the
<Liquid Detector Test Results> window.

Figure 4.151:<Liquid Detector Test Results> Window

12. After finishing the test, the message <Liquid Detector


Test passed> or <Liquid Detector Test failed> displays.

Figure 4.152:<Liquid Detector Test Passed>

13. Confirm with <OK>. The <Liquid Detector Test Results>


window with final test results is active.
14. Close the window with <Close>. The <Liquid Detector
Test Configuration> dialog is active again.
15. Finish the test with <Close>.
16. The test passed if <Tot Errs = 0>.
17. If the test fails:
• Verify all tips have the same height; if not, correct
with <LiHa>, <Setup>, <Individual Z>.
• Check the liquid system for air bubbles.

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Section 4 Set Up Liquid System

Perform Backup
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The EEPROM function allows backup of the EEPROM
parameters of one or more devices on disk. This also allows
parameters to be restored from disk. For example, performing a
backup of the current device parameters is recommended if an
electronic board must be replaced. Restoration of parameters
after the board is replaced eliminates redo of the setup and/or
calibration procedures.

Figure 4.153: Setup Panel

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The EEPROM page includes the controls in the following


table:
Table 4.4: EEPROM Controls

Control Description
Do for Checkboxes to select the device addresses of
the EEPROMs to backup.
Device Name Indicates the subject device.
Restore with Displays the names of the files selected with
the <Browse […]> buttons.
Browse […] Command buttons that open the standard
Windows <Open> dialog to search the files
with parameters to restore.

EEPROM Backup
1. On the <Contents> page of the <Instrument>, <Basic
Setup> menu, select the <EEPROM Backup/Restore>
checkbox (聺) and switch to the <EEPROM> page.

Figure 4.154:<Contents> Page

2. When the EEPROM page displays, select the checkboxes of


the EEPROMs to perform backup.
NOTE: At minimum, backup the key instrument
parameters: M1, O4, O5, C1, O2, and O3.

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Figure 4.155:EEPROM Page

3. Select <Start>. Contents of the selected EEPROMs are


written to disk.

Exiting Setup and Service


The next step in the installation process is calibrating the LiHa
and RoMa using m2000sp software. It will be necessary to exit
S&S and allow the system to reboot and return to the m2000sp
software before proceeding.

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LiHa Calibrations
LiHa calibrations are categorized by the implement used to
perform them. The <Calibrate Liquid Handler> menu offers
three calibration options based on those implements:
• Tip Adapter
• Reference Tip
• Disposable Tip

To access m2000sp calibration commands, go to <System>


then <System Calibration>.

Figure 4.156: System Calibration Status Screen

WARNING: Potential Biohazard. If calibrations


are performed at any time other than installation,
instrument parts might be contaminated with
potentially infectious materials. Observe basic
biohazard precautions. Wear appropriate personal
protective equipment such as gloves, lab coats,
and protective eyewear.
Password Protection: These procedures are
exclusively for FSEs and require use of the
appropriate password.
NOTE: Additional calibrations may need to be
completed. See appropriate TSBs.

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Section 4 LiHa Calibrations

The following table lists the three types of LiHa calibrations


performed and the worktable locations calibrated with each.
Table 4.5: LiHa Calibrations

Type Worktable Location


• DiTi racks
Calibrate Tip • DiTi reuse racks
Adapter Locations • Deep well plate (X-Y positioning)
• PCR plate (X-Y positioning)
• 1 mL Subsystem locations
• ASR location
Calibrate Reference
• Master Mix Tube
Tip Locations
• Deep well plate (Z positioning)
• PCR plate (Z positioning)
• 13 mm tube (grid 2)
• 16 mm tube (grid 3)
• Transport tube (pierceable cap)
(grid 4)
• Custom 13 mm tube (grid 2)
Calibrate • Custom 16 mm tube (grid 3)
Disposable Tip NOTE: Before performing
Locations calibrations with DiTis, the
sample racks and tubes the
customer will use must be
properly configured. Refer to
Sample Rack and Tube
Configuration on page 4–149
for more information.

At the completion of LiHa calibrations, verification procedures


must be performed. Refer to Calibration Verifications on page
4–167.

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Calibrate Tip Adapter Locations


During these procedures, the LiHa will be moved to each of
the DiTi racks and reuse racks on the worktable, and to the
PCR and deep well plates on the Output Deck, and will then be
aligned with well A1 of each tray or plate.

Figure 4.157: Tip Adapter Properly Aligned in A1

1. Perform the following steps to set up the worktable:


• Load two 1000 µL DiTi racks on DiTi rack carrier 1
after removing the DiTi from well A1 of each
• Load two 200 µL DiTi racks on DiTi rack carrier 2 after
removing the DiTi from well A1 of each
• Load empty DiTi trays in the DiTi reuse racks on both
DiTi rack carriers
• Load a PCR plate on the front position of the Output
Deck and a deep well plate in the middle position
2. From the main menu select <System> then <System
Calibration>.

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3. Select <Tip Adapter> from the <Calibrate Liquid


Handler> menu on the left side of the screen. The <Liquid
Handler Calibration> screen displays.

Figure 4.158: Liquid Handler Calibration Screen

4. Align the tip adapter in the X and Y direction using the


arrow keys and the <Safe Move> button.
NOTE: The LiHa will not move until <Safe Move>
is selected.
5. Select <Move>.
6. Use the arrows to align the tip adapter to the target. Adjust
the X and Y directions first, followed by the Z. The
<Move> button will need to be selected after each change
to express the new coordinates and to save them.
In the X and Y directions, the tip adapter cone should be
centered in the target.
In the Z direction, the bottom of the black tip ejector tube
should barely touch the surface.
IMPORTANT: For Z adjustments, do NOT drive
! down and then step up, as this will magnify
hysteresis problems. Step down to the calibration
point. If you do need to step up, select <Home>
and then <Move> to return to the calibration
point.

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Figure 4.159: Tip Adapter Properly Aligned with Deep Well


Plate

7. Select <Home>, then <Move>. Repeat the alignments if


necessary. Once the adjustments are complete, select
<Move> a final time. Then select <Next> to move to the
next calibration location.
8. When all calibrations are complete, select <Finish> then
<Close> to return to the <System Calibration> screen.

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Section 4 LiHa Calibrations

Calibrate Reference Tip Locations


There are two types of reference tips available; either may be
used for calibration. Refer to Reference Tips on page 4–54 for
more information.

1 mL Subsystem Calibration
There are two stages to 1 mL Subsystem calibration. First,
heater zone 1, heater zone 2, the room temperature zone, and
the magnetic zone (MagLow) are calibrated, then using a 1 mL
Subsystem carrier, MagRest is calibrated.

1. Perform the following steps to prepare the 1 mL


Subsystem:
• Remove any carriers present
• To assist in visualizing the target, use a black marker
to color the target indentation in the center of each
zone for contrast
2. From the main menu select <System> then <System
Calibration>.
3. Select <Reference Tip> from the <Calibrate Liquid
Handler> menu on the left side of the screen. The <Liquid
Handler Calibration> screen displays.

Figure 4.160: Liquid Handler Calibration Screen

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4. Follow the prompts to mount the reference tip on LiHa


tip 3.

Figure 4.161: New Reference Tip Mounted on Tip 3

5. For each location, select <Safe Move> to align LiHa tip 3


with the calibration target.
6. Align the reference tip in the X and Y direction using the
arrow keys and the <Safe Move> button.
NOTE: The LiHa will not move until <Safe Move>
is selected.
7. Select <Move>.
8. Use the arrows to align the reference tip to each
calibration indentation. Adjust the X and Y directions
first, followed by the Z. The <Move> button will need to
be selected after each change to express the new
coordinates and to save them.
In the X and Y directions, the reference tip should be
centered in the calibration target.

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In the Z direction, the reference tip should barely touch.


Use a piece of paper between the tip and target to gauge
resistance.

Figure 4.162: Reference Tip and Paper

IMPORTANT: For Z adjustments, do NOT drive


! down and then step up, as this will magnify
hysteresis problems. Step down to the calibration
point. If you do need to step up, select <Home>
and then <Move> to return to the calibration
point.
9. Select <Home>, then <Move>. Repeat the alignments if
necessary. Once the adjustments are complete, select
<Move> a final time. Then select <Next> to move to the
next calibration location.
10. After the first four zones (heater 1, heater 2, room
temperature, and MagLow) are calibrated, place a 1 mL
Subsystem carrier (without cover) in the MagRest position.
11. Select <Safe Move> to align LiHa tip 3 above the center of
the raised carrier.
IMPORTANT: For carriers with crosshair targets in
! the center, do NOT attempt to calibrate to the
crosshair. Calibrate only according to the
directions in this section.
12. Move the X position to the right 20 steps to avoid settling
in the groove.
13. Select <Move>.
14. Use the arrows to align the reference tip to the target. Only
the Z should need to be adjusted.

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15. Check the location values for X and Y. The X value should
be the same as MagLow X. The Y value should be 50 steps
greater than the MagLow Y value.

Figure 4.163:Carrier in MagRest Position

NOTE: The <Move> button will need to be


selected after each change to express the new
coordinates and to save them.
In the Z direction, the reference tip should barely touch
the surface. Use a piece of paper between the tip and
target to gauge resistance.
16. When the tip is calibrated in the Z, move the X direction
back to the left 20 steps.
17. Select <Home>, then <Move>. Repeat the alignments if
necessary. Once the adjustments are complete, select
<Move> a final time. Then select <Next> to move to the
next calibration location, the assay-specific reagent
holder.

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ASR Calibration
1. Mount the ASR Calibration tool on the assay reagent
holder.

Figure 4.164: ASR Calibration Tool and Target

NOTE: If you are not already in the process of


performing reference tip calibrations, select
<System>, <System Calibration>, and then
<Reference Tip> from the <Calibrate Liquid
Handler> menu. Follow the prompts to mount
the reference tip, and then select <Next> until you
reach screen 6 of 9.
2. Select <Safe Move> to align LiHa tip 3 with the calibration
target, an indentation in the upper-left corner of the ASR
tool.
3. Align the reference tip in the X and Y direction using the
arrow keys and the <Safe Move> button.
NOTE: The LiHa will not move until <Safe Move>
is selected.
4. Select <Move>.
5. Use the arrows to align the reference tip to the target.
Adjust the X and Y directions first, followed by the Z. The
<Move> button will need to be selected after each change
to express the new coordinates and to save them.

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In the X and Y directions, the reference tip should be


centered in the calibration target.
In the Z direction, the reference tip should barely touch.
Use a piece of paper between the tip and target to gauge
resistance.
IMPORTANT: For Z adjustments, do NOT drive
! down and then step up, as this will magnify
hysteresis problems. Step down to the calibration
point. If you do need to step up, select <Home>
and then <Move> to return to the calibration
point.
6. Select <Home>, then <Move>. Repeat the alignments if
necessary. Once the adjustments are complete, select
<Move> a final time. Then select <Next> to move to the
next calibration location, the master mix tube.

Master Mix Tube Calibration


NOTE: If you are not already in the process of
performing reference tip calibrations, select <System>,
<System Calibration>, and then <Reference Tip> from
the <Calibrate Liquid Handler> menu. Follow the
prompts to mount the reference tip, and then select
<Next> until you reach screen 7 of 9.
1. Insert the master mix tube upside down at master mix
location 1.

Figure 4.165: Master mix tube inserted upside down

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2. Select <Safe Move> to align LiHa tip 3 with the calibration


target.
IMPORTANT: While the tube is upside down DO
! NOT select <MOVE>! Otherwise, a Drive No Load
error will occur and the instrument will have to be
reinitialized.
3. Manually raise the tube up to the bottom of the reference
tip.
4. Make X- and Y-axis adjustments as needed to center the
tip.
5. Select <Safe Move>.
NOTE: The LiHa will not move until <Safe Move>
is selected.
6. After the X and Y axis have been centered, select <Home>.
7. Select <Safe Move> and verify X and Y alignment. Make
adjustments as needed as described in steps 3 and 4.
8. Select <Home>.
9. Remove the master mix tube, turn it upright, and insert it
in its normal position.

Figure 4.166: Master mix tube positioned correctly

10. Select <Safe Move> and then <Move>

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11. Adjust the Z-axis to the well bottom, making minor


adjustments to X and Y if needed so the tip is in the center
of the tube.
In the Z direction, the reference tip should barely touch
the bottom of the tube. Use your fingers to try and raise
the tube. There should be a very small amount of
movement (1-2 steps) in the Z direction.

Figure 4.167: Checking Z-direction movement

IMPORTANT: For Z adjustments, do NOT drive


! down and then step up, as this will magnify
hysteresis problems. Step down to the calibration
point. If you do need to step up, select <Home>
and then <Move> to return to the calibration
point.
12. Select <Home>, then <Move>. Repeat the alignments if
necessary. Once the adjustments are complete, select
<Move> a final time. Then select <Next> to move to the
next calibration location, the deep well and PCR plates on
the Output Deck.

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Deep Well Plate and PCR Plate Calibration


1. Remove the deep well plate and PCR plate from the
Output Deck.
NOTE: If you are not already in the process of
performing reference tip calibrations, select
<System>, <System Calibration>, and then
<Reference Tip> from the <Calibrate Liquid
Handler> menu. Follow the prompts to mount
the reference tip, and then select <Next> until you
reach screen 8 of 9.
2. Select <Safe Move> to align LiHa tip 3 with the calibration
target on the PCR plate, which in this case is any flat
surface on the plate, away from the dimples.
NOTE: X and Y are not particularly important for
these calibrations. The Z is the critical calibration
point.

Figure 4.168: Reference Tip Positioned on Flat Surface

3. Align the reference tip in the X and Y direction using the


arrow keys and the <Safe Move> button.
NOTE: The LiHa will not move until <Safe Move>
is selected.
4. Select <Move>.
5. Use the arrows to align the reference tip to the target.
Adjust the X and Y directions first, followed by the Z. The
<Move> button will need to be selected after each change
to express the new coordinates and to save them.
In the X and Y directions, the reference tip should be
away from the dimples.

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In the Z direction, the reference tip should barely touch


the surface. Use a piece of paper between the tip and
target to gauge resistance.
IMPORTANT: For Z adjustments, do NOT drive
! down and then step up, as this will magnify
hysteresis problems. Step down to the calibration
point. If you do need to step up, select <Home>
and then <Move> to return to the calibration
point.
6. Select <Home>, then <Move>. Repeat the alignments if
necessary. Once the adjustments are complete, select
<Move> a final time.
7. After calibrating the PCR plate, select <Next> and repeat
the above steps for the deep well plate.
8. When the PCR and deep well plates are calibrated, select
<Finish> then <Close> to return to the <System
Calibration> screen.

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Section 4 LiHa Calibrations

Sample Rack and Tube Configuration


Prior to calibrating the disposable tip locations, the sample
racks and tubes must be configured as described in the
following procedures.

Enabling a Rack
Sample tube size and style (spherical, conical, or flat bottom)
configurations must be set by an FSE at installation. Perform
the following steps to properly configure a system:
1. Navigate to <System>,<Settings>, and then <Sample
Rack and Tube>.
2. Select <Configure> from the <Setting Tasks> menu.

Figure 4.169: Sample Rack and Tube Configuration

The screen covers three rack types under <Rack ID>:


• 13 mm
• 16 mm
• Pierceable cap (transport tube)
For the 13 mm and 16 mm racks, customers have the
option to use the standard (<Default>) rack and tube
configurations or choose a custom setup of their own
(<User Defined>).

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Installation Section 4

3. If the customer will be using only the standard m2000sp


rack and tube configuration, or the Pierceable Cap rack,
verify that <Default> is checked in the applicable field,
then select <Save>.

Figure 4.170: 13 mm Rack with Standard Configuration

4. To enable a custom rack, first select <User Defined> in the


applicable field (13 mm or 16 mm).

Figure 4.171: 13 mm Rack with Standard and Custom


Configurations Selected

5. Next, make a selection from the <Bottom Type> menu by


clicking on the toggle and scrolling down the list.

Figure 4.172: Bottom Type Menu

NOTE: Conical, non-cylindrical, or skirted tubes


(also referred to as false bottom or self-standing)
should not be used on the m2000sp unless
specifically approved by Abbott Molecular.
Contact Abbott Molecular GSS for assistance.

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Section 4 LiHa Calibrations

6. After defining the type of tube to be used, the tube’s outer


diameter must be measured and entered into the <Outer
Diameter> field.

Figure 4.173: Outer Diameter Field

NOTE: For 13 mm tubes, the outer diameter must


be between 11.5 and 14 mm. Tubes outside this
range cannot be used.
For 16 mm tubes, the outer diameter must be
between 14.5 and 16 mm. Tubes outside this range
cannot be used.

7. If a conical tube was selected under <Bottom Type>, the


bottom height will have to be determined and the
calculated value entered into the <Bottom Height> field.
Contact Abbott Molecular GSS for assistance in making
this determination.

Figure 4.174: Bottom Height Field

NOTE: The bottom heights for spherical and


flat-bottom tubes are automatically entered into
the <Bottom Height> field once the <Outer
Diameter> field is completed.

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Installation Section 4

8. After all configurations have been made, select <Save>.


The newly enabled racks must now be calibrated. Proceed
to Calibrate Disposable Tip Locations on page 4–153.
NOTE: Recalibration is also required whenever
previously enabled racks have had their
dimensions or other parameters changed.
After calibration is complete, run the LiHa Sample Tube
Check on page 5–14 of Section 5.
9. Attach the correct PoSt and label to each enabled rack,
ensuring the bar code faces the rear of the instrument.
Table 4.6: Sample Rack Bar Code Labels

Sample Rack Bar Code PoSt Label Color


13mm (blue insert) 013/xxxxxx Blue
16mm 016/xxxxxx White
Pierceable cap 513/xxxxxx Red
Custom 13 mm 713/xxxxxx Blue with an orange
stripe
Custom 16 mm 716/xxxxxx White with an
orange stripe

Disabling a Rack
To disable a previously enabled rack, deselect its check box
under <Rack Enable> and select <Yes> at the prompt.

Figure 4.175: Check Boxes

NOTE: Once a rack is disabled, the calibration for that


rack will be lost, even if it is later re-enabled.
Calibration will be required to regain valid status if the
rack is re-enabled.

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Section 4 LiHa Calibrations

Calibrate Disposable Tip Locations


During these procedures, the LiHa will be moved to each of
the sample input racks used on the system and aligned with
the tube in position 1 of each rack.
Before performing calibrations with DiTis, the system must
have an accurate understanding of the DiTi racks present on
the worktable. The <Disposable Tip Status> display, found
under the <System> menu, shows the system’s understanding
of the DiTis remaining on the shelves and worktable after each
run.
NOTE: The information displayed here is NOT the
result of specific inventory sensors. It is a reflection of
the system’s memory of what tips it has used to this
point.
If the tip status is not updated after additions are made,
the system will not recognize the DiTi racks that were
added and, as a result, will bypass them during the next
run.
Any time DiTi racks are added to the worktable or shelves, it
will be necessary to update the system by selecting one of the
options under <Update>.

Figure 4.176: <Update> Menu Options

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Installation Section 4

1. Perform the following steps (as applicable to your system)


to set up the worktable for DiTi location calibrations:
• Ensure there is at least a partially full rack of 1000 µL
DiTis on DiTi rack carrier 1, location 1 or 2, and that
the <Disposable Tip Status> screen reflects what is
physically present on the worktable.
• Load a 13 mm sample rack with a single sample tube
in position 1 and place the rack in grid position 2
NOTE: If the customer will be using both default
and custom tubes, load the default rack first and
run the procedure, then, at the screen prompt
switch out the racks. This applies to all tube sizes.
• Load a 16 mm sample rack with a single sample tube
(default first) in position 1 and place the rack in grid
position 3
• Load a transport tube (pierceable cap) rack with a
single sample tube (with the cap removed) in position
1 and place it in grid position 4
IMPORTANT: For each sized tube above, the
! largest round-bottom tube used by the customer
should be used here.
2. From the main menu select <System> then <System
Calibration>.
3. Select <Disposable Tip> from the <Calibrate Liquid
Handler> menu on the left side of the screen. The <Liquid
Handler Calibration> screen displays.

Figure 4.177: Liquid Handler Calibration Screen

4. At the prompt, select <Close> to allow the LiHa to move


and the system to stake a DiTi on LiHa tip 3.

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Section 4 LiHa Calibrations

5. For each location, select <Safe Move> to align LiHa tip 3


with the calibration target.
6. Align the tip adapter in the X and Y direction using the
arrow keys and the <Safe Move> button.
NOTE: The LiHa will not move until <Safe Move>
is selected.
7. Select <Move>.
8. Use the arrows to align the DiTi to the target. Adjust the X
and Y directions first, followed by the Z. The <Move>
button will need to be selected after each change to
express the new coordinates and to save them.
In the X and Y directions, the tip should be centered in
the tube.
In the Z direction, the tip should barely touch the bottom
of the tube. Use your fingers to try and raise the tube.
There should be a very small amount of movement (1-2
steps) in the Z direction.

Figure 4.178: Checking Z-direction Movement

IMPORTANT: For Z adjustments, do NOT drive


! down and then step up, as this will magnify
hysteresis problems. Step down to the calibration
point. If you do need to step up, select <Home>
and then <Move> to return to the calibration
point.

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Installation Section 4

9. Select <Home>, then <Move>. Repeat the alignments if


necessary. Once the adjustments are complete, select
<Move> a final time. Then select <Next> to move to the
next location.
10. After calibrating the first tube type, repeat the above steps
for all remaining tubes.
11. When all tubes are calibrated, select <Finish> then
<Close> to return to the <System Calibration> screen.

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Section 4 RoMa Calibrations

RoMa Calibrations
The left-side <Calibrate Robotic Manipulator> menu offers
three calibration options:
• 1 mL Subsystem Calibration, found on page 4–158
• Disposable Tip Rack Calibration, found on page 4–161
• Shelf Calibration, found on page 4–165

To access m2000sp calibration commands, go to <System>


then <System Calibration>.

Figure 4.179: System Calibration Status Screen

WARNING: Potential Biohazard. If calibrations


are performed at any time other than installation,
instrument parts might be contaminated with
potentially infectious materials. Observe basic
biohazard precautions. Wear appropriate personal
protective equipment such as gloves, lab coats,
and protective eyewear.
Password Protection: These procedures are
exclusively for FSEs and require use of the
appropriate password.
NOTE: Additional calibrations may need to be
completed. See appropriate TSBs.

At the completion of RoMa calibrations, verification


procedures must be performed. Refer to Calibration
Verifications on page 4–167.

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Installation Section 4

1 mL Subsystem Calibration
For each calibration point, the RoMa gripper arms will be
properly aligned with the 1 mL Subsystem carrier.
IMPORTANT: It should NOT be necessary to
! adjust the Y-axis value during RoMa calibration.
Adjusting the Y value in one location might affect
the value at all locations. Therefore, if a Y change
is made in one location, the same change must be
made in all locations.
NOTE: The gripper open distance should be
adjusted minimally. These values will be less than
those seen in m2000sp Software Version 1.1 in
order to accommodate 96 sample processing.
1. Load a 1 mL Subsystem carrier with RVs, install the cover,
and place the carrier in the target area, beginning with
heater zone 1 and moving from location to location as
directed by the on-screen prompts.
The carrier must contain RVs so that deflection can be
observed if it occurs.
2. From the main menu select <System> then <System
Calibration>.
3. Select <1 mL Subsystem> from the <Calibrate Robotic
Manipulator> menu on the left side of the screen. The
<Robotic Manipulator Calibration> screen displays.

Figure 4.180: Robotic Manipulator Calibration Screen

4. Select <Safe Move>.

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Section 4 RoMa Calibrations

5. Adjust the RoMa arms in the X direction only using the


arrow keys and the <Safe Move> button.
NOTE: The RoMa will not move until <Safe
Move> is selected.
6. Select <Move>.
7. Use the arrows in the X and Z positions to align the RoMa
arms with the carrier. X should be adjusted first, then Z.
The <Move> button will need to be selected after each
change to express the new coordinates and to save them.
The goal is to have the RoMa gripper arms positioned just
below the lip on the sides of the carrier, and properly
centered on both sides.

Figure 4.181: RoMa Gripper Properly Positioned

8. Select <Home>, then <Safe Move>, then <Move>.


9. To verify the position, use the <Grip> button to grip the
carrier and the <Raise / Lower> button to observe any
deflection of the RVs. Select <Release> once the carrier has
been lowered.
NOTE: <Release> and <Lower> do not appear as
options until <Grip> or <Raise> are selected.
10. Repeat the alignment if necessary.
11. Once the adjustments are complete, select <Move> a final
time to save the coordinates, and then <Next> to move to
the next 1 mL Subsystem location.

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Installation Section 4

12. After all locations have been calibrated, select <Finish>


then <Close> to return to the <System Calibration>
screen.

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Section 4 RoMa Calibrations

Disposable Tip Rack Calibration


DiTi rack calibration is a two-step process. The first step is for Z
and the second step is for X and Y.
1. Perform the following steps to prepare the worktable:
• Remove DiTi carrier 2 from grids 27-32, and remove
the reagent carriers from grids 33-34
• Remove both DiTi racks from DiTi carrier 1, but leave
the carrier in place on grids 21-26
2. From the main menu select <System> then <System
Calibration>.
3. Select <Disposable Tip Racks> from the <Calibrate
Robotic Manipulator> menu on the left side of the
screen. The <Robotic Manipulator Calibration> screen
displays.

Figure 4.182: Robotic Manipulator Calibration Screen

4. Select <Safe Move>.


5. Adjust the RoMa arms in the Z direction only using the
arrow keys and the <Safe Move> button.
NOTE: The RoMa will not move until <Safe
Move> is selected.
6. Select <Move>.

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Installation Section 4

7. Use the arrows in the Z position to align the RoMa arms


with the carrier surface. The <Move> button will need to
be selected after each change to express the new
coordinates and to save them.
The goal is to have the bottom of the gripper pads just
touch the surface of the carrier.
NOTE: Position 1 might be difficult to see because
one pad will be on the rounded edge. If necessary,
use the value for Position 2.

Figure 4.183: RoMa Gripper Aligned with Surface

8. Select <Home>, then <Safe Move>, then <Move>.


9. Repeat the alignment if necessary.
10. Once the adjustments are complete, select <Move> a final
time to save the coordinates, and then <Next> to move to
the next position.
11. Select <Safe Move>.
12. Place a 1000 µL DiTi rack in the back position of DiTi
carrier 1.

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Section 4 RoMa Calibrations

13. Use the arrows in the X and Y positions to align the RoMa
arms with the rack. The <Move> button will need to be
selected after each change to express the new coordinates
and to save them.
The goal is to have the RoMa gripper arms positioned just
below the lip on the sides of the carrier, and properly
centered on both sides. In the Y-axis, the gripper pins
should be equidistant to the sides of the DiTi rack and
should just touch the end of the rack.

Figure 4.184: RoMa Gripper Aligned with Rack

14. Select <Home>, then <Safe Move>, then <Move>.


15. To verify the position, use the <Grip> button to grip the
carrier and the <Raise / Lower> button to observe the
movement. Select <Release> once the carrier has been
lowered.
NOTE: <Release> and <Lower> do not appear as
options until <Grip> or <Raise> are selected.
16. Repeat the alignment if necessary. Once the adjustments
are complete, select <Move> a final time to save the
coordinates, and then <Next> to move to the next
position.
17. Remove the DiTi rack from the carrier and repeat the
above steps for the carrier’s middle position.

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Installation Section 4

18. After all locations have been calibrated, select <Finish>


then <Close> to return to the <System Calibration>
screen.

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Section 4 RoMa Calibrations

Shelf Calibration
For each calibration point, the RoMa gripper arms will be
properly aligned with the shelf rack positions.
1. Load a 1000 µL DiTi rack on DiTi shelf 8. Push the rack in
until it just hits the rise at the rear of the shelf.
IMPORTANT: Ensure that the rack is NOT aligned
! with the front edge of the shelf.
2. From the main menu select <System> then <System
Calibration>.
3. Select <Shelves> from the <Calibrate Robotic
Manipulator> menu on the left side of the screen. The
<Robotic Manipulator Calibration> screen displays.

Figure 4.185: Robotic Manipulator Calibration Screen

4. Select <Safe Move>.


5. Adjust the RoMa arms in the X and Y directions using the
arrow keys and the <Safe Move> button.
NOTE: The RoMa will not move until <Safe
Move> is selected.
6. Select <Move>.

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Installation Section 4

7. Use the arrows in the X, Y, and Z positions to align the


RoMa arms with the shelf. X and Y should be adjusted
first, then Z. The <Move> button will need to be selected
after each change to express the new coordinates and to
save them.
The goal is to have the RoMa gripper arms centered on
both sides of the rack and just making contact with the
surface of the shelf. The gripper pins should just touch
the end of the DiTi rack.

Figure 4.186: RoMa Gripper Properly Positioned on Shelf 8

8. Select <Home>, then <Safe Move>, then <Move>.


9. Repeat the alignment if necessary.
10. Once the adjustments are complete, select <Move> a final
time to save the coordinates, and then <Next> to move to
the next shelf.
IMPORTANT: Be consistent across the shelf
! calibrations. Make adjustments as necessary based
on the RoMa DiTi Rack Test on page 5–27 and
RoMa DiTi Shelf Test on page 5–29 of Section 5,
Maintenance and Diagnostic Procedures.
11. After all shelves have been calibrated, select <Finish> then
<Close> to return to the <System Calibration> screen.

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Section 4 Calibration Verifications

Calibration Verifications
M&D Procedures
To verify all LiHa and RoMa calibrations the following M&D
Procedures should be performed:
• Extensive Flush on page 5–10
• Dispense Test 50 µL, Gravimetric on page 5–23
• Dispense Test 800 µL, Gravimetric on page 5–26
• LiHa DiTi Location Test on page 5–12
• LiHa Sample Tube Check on page 5–14
• DiTi Rack Checks – Deck and Shelf
– RoMa DiTi Rack Test on page 5–27
– RoMa DiTi Shelf Test on page 5–29
• RoMa 1 mL Carrier Test on page 5–30
• Sample Extraction Test on page 5–37
• Z-Calibration Checks
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
All M&D procedures can be found in Section 5, Maintenance
and Diagnostic Procedures.

Print Calibrations Report


After all M&D verifications have passed, print and keep copies
of the Liquid Handler and Robotic Manipulator reports found
under the <Print Report> menu located on the lower-left side
of the main calibration page, <System Calibration Status>.
IMPORTANT: The RoMa gripper calibration
! coordinates are incorrect in this report. If the G-axis is
altered in any way during calibration, the FSE must
write the new coordinates on the printed report.

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Installation Section 4

Network Configuration
Install the Router
IMPORTANT: For AbbottLink router installation, refer
! to the latest revision of the AbbottLink ISA.

Perform the following steps to install the router:

Rename the SCC


For each SCC to be connected to the router:
1. From the m2000sp or m2000rt menu, navigate to
<System><Explorer>.
2. Navigate to and highlight <My Computer>
3. Right click and select <Properties>
4. Navigate to the <Computer Name> tab
5. Select the <Change...> button. The Computer Name
Changes dialog will appear.
6. In the <Computer name> field, enter the following:
a. m2000sp-1 for m2000sp SCC #1
b. m2000sp-2 for m2000sp SCC #2
c. m2000sp-3 for m2000sp SCC #3
d. m2000sp-4 for m2000sp SCC #4
e. m2000rt-1 for m2000rt SCC #1
f. m2000rt-2 for m2000rt SCC #2
g. m2000rt-3 for m2000rt SCC #3
h. m2000rt-4 for m2000rt SCC #4
7. Select <Ok>, <Ok>, <Ok>.
8. A System Settings Change dialog will appear. Select <No>
to reboot the SCC.
9. Another dialog will appear asking if you wish to exit the
application. Select <Yes>.

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Section 4 Network Configuration

Router Configuration

Figure 4.187: Router

1. Power off the SCC to each system that is to be connected


to the router.
2. Connect one end of an RJ-45 cable into the Ethernet
connection on SCC #1 (first system) and the other end
into LAN port 1 on the router.

LAN ports 4 3 2 1

Figure 4.188: Router LAN ports 4 though 1

3. Connect each additional instrument into the remaining


ports (2, 3 and 4)
4. Connect the power cable to the hardware router and
power up the router.
5. Power on all SCCs connected to the router.

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Installation Section 4

Network Configuration Screen


Regardless of the method of network connection (crossover
cable, router, etc.), the network connection will need to be
configured after the connection is established. From the
<System Settings> menu, select <Network Configuration>
then <Configure>.

Figure 4.189: Network Configuration screen

If no network connection will be used, select <No network


connection (export via CD only)> if it is not already chosen.
If a network connection is to be used, choose one of the
following options:
• <Send to m2000rt via crossover cable>, which allows the
instrument to be connected directly to one m2000rt via a
crossover cable; the exported Test Orders are stored on the
connected m2000rt system. Proceed to Crossover Cable on
page 4–171.
• <Send to m2000rt2.0 via network connection>, which
allows the instrument to be connected directly to one
m2000rt with release 2.0 via a network connection; the
exported Test Orders are stored on the connected m2000rt
system. Proceed to Connect with m2000rt 2.0 on page
4–171.

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Section 4 Network Configuration

• <Send to network data store>, which allows the


instrument and other m2000sps to be connected to one or
more m2000rts in a multi-instrument network; the
exported Test Orders are stored on any of the connected
systems in the network or on an AbbottLink computer, if
available. Proceed to Network Data Store on page 4–171.
NOTE: The Network Data Store option is only
available with an m2000rt system with release 2.1
software.

Crossover Cable
1. Enter “m2000rt-1” as the workgroup name.
2. Test the configured connection by selecting the <Test>
button.
3. Select <Save>.

Connect with m2000rt 2.0


1. Enter “m2000rt-1” as the IP address.
2. Test the configured connection by selecting the <Test>
button.
3. Select <Save>.

Network Data Store


For this option, both the m2000sp and m2000rt systems will
need to be configured.
On the m2000sp:
1. Enter the IP address of the m2000rt, m2000sp, or
Abbottlink computer that will be used as the Network
Data Store.
To find the IP address, do the following:
• For the m2000rt, navigate to the <Serial Number
Configuration> screen.
• For the m2000sp, navigate to the <Hardware
Configuration> screen.
• For an Abbottlink computer, refer to the Abbottlink or
Router Installation Survey.
2. Test the configured connection by selecting the <Test>
button.

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Installation Section 4

3. Select <Save>.
On the m2000rt systems:
1. Navigate to the <System><Settings><Network
Configuration> screen.

Figure 4.190:m2000rt Network Configuration screen

2. Select the <Get from network data store> option.


3. Enter the IP Address that was entered above for the
m2000sp systems.
4. Select <Test>.

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Section 4 Database Management

Database Management
There are six backup options provided on the <Database
Management> menu:
• Full backup, which combines the System Database and
System Calibrations options, and includes all system data
• System Database only, which includes all database
information (including result information)
• System Calibrations only, which includes all data related
to LiHa and RoMa calibrations
• Configuration Files, which includes only system
configuration data
• System Logs, which include all log files except those from
Genesis
• Calibration Data, which only includes absolute
coordinates of previous calibrations
NOTE: All six categories can be backed up on a
CD; however, only the first three (Full, System
Database, and System Calibration) can be restored.
The last three options are backed up for
troubleshooting purposes only.

System File Backup


1. To perform a file backup within the m2000sp software, log
in as an FSE.
2. Navigate to <System>, <Database Management>.

Figure 4.191:<Database Management> Screen

3. Highlight a backup option and select <Backup> from the


<Database Management Tasks> menu.

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Installation Section 4

The <Perform System Backup> screen displays, giving


you the option to enter a comment if you choose.
4. Load a blank CD into the CD drive. Then select <Start>.

Figure 4.192:Backup

5. Leave a copy of the backup CD at the site in the front


pocket of the m2000sp Operations Manual.

System File Restore


The m2000sp system offers the following restore options:
• Full
• System Database
• System Calibrations
NOTE: The remaining backup options (Configuration
Files, System Logs, and Calibration Data) cannot be
restored. They are backed up for troubleshooting only.

To restore a system file, do the following:


1. Insert the CD containing the files to be restored.
NOTE: The CD must be in the drive and the CD
light off prior to selecting <Restore>.
2. Wait until the drive finishes spinning (approximately 12
seconds).

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Section 4 Database Management

3. Select <Restore> from the <Database Management


Tasks> menu.

Figure 4.193: System Restore Display

4. Highlight the backup files in the <Select Backup> field


and select <Start>.
NOTE: Here, the previous backup dictates what is
restored. For example, if you did a Full backup, the
restore would do a Full restore, even if you select
System Calibrations only.
NOTE: The RAD945 system has timing issues with
system restore functions.
If you experience problems, such as system lockup
or a big red X on the backup listing screen, reboot
the SCC and retry the restore.

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Installation Section 4

On-line Documentation
Load the on-line documentation after downloading all
necessary files from the GSS website’s Software Update page.

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Section 4 System Settings

System Settings
To access the <System Settings> menu, select <System> then
<Settings>. This menu offers ten possible options:
• Hardware Configuration. Refer to Hardware
Configuration on page 4–44.
• Audible Alert
• Time and Date
• Sample Bar Code
• Sample Rack and Tube. Refer to Sample Rack and Tube
Configuration on page 4–149.
• Run Checks
• Signal Tower
• Language and Locale
• Network Configuration. Refer to Network Configuration
Screen on page 4–170.
• Automatic Export

NOTE: The Signal Tower option will appear only if that


device has been enabled by the FSE on the <Hardware
Configuration> screen. Refer to Hardware
Configuration on page 4–44.

Audible Alert
NOTE: The following procedure can also be performed
by customers.
This screen allows the user to:
• enable or disable the audible alert when an alert
dialog is generated
• choose a sound scheme
• adjust the volume for operator notifications

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Installation Section 4

1. To configure the audible alert, highlight <Audible Alert>


on the <System Settings> screen and then, from the task
menu, select <Configure>.
2. On the <Audible Alert Configuration> screen, select a
sound scheme for each message type to be enabled:
• <Error>
• <Warning>
• <Information>
To disable a sound scheme, choose <none> from the
respective message type’s dropdown menu.

Click here to reveal


the dropdown menu

Click here to hear


the sound sample

Select [none] to disable


sound for this message

Figure 4.194:Choosing a sound scheme

NOTE: Refer to the ISA database for instructions


regarding customer-desired custom sound schemes.
3. Select <Test> to hear the selected sound scheme.
4. Choose the volume level desired by using the arrows on
the <Volume> toggle at the top of the screen. The volume
setting can be verified by clicking <Test>.

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Figure 4.195:<Audible Alert Configuration> Display

NOTE: The volume on the monitor may also need


to be adjusted.
NOTE: Contact your Abbott Representative if
external speakers are desired.

5. Click <Save> when finished.

Time and Date


NOTE: The following procedure can also be performed
by customers with administrator access.
To set the correct time and date, and time zone select
<Settings> from the <System> menu, highlight <Time and
Date>, and choose <Configure> from the <Setting Tasks>
menu.

Figure 4.196:<Time and Date Configuration>

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Installation Section 4

To Set Time and Date


Select the desired field, and then use the toggle at the right to
move the setting up or down as necessary. Click <Save> when
finished.

Figure 4.197: Setting Time and Date

NOTE: Daylight savings settings are configured by the


FSE as part of the Installation TSB.
If you have not already configured for daylight savings,
select <System>, <Explorer>,<My Computer>, and
<Control Panel>. Then:
1.Select <Date, Time, Language, and Regional
Options> and then <Date and Time> from the
control panel icon list.
2.Select the <Time Zone> tab and select or deselect
<Automatically adjust clock for daylight savings
changes> per the locale.
NOTE: The date and time formats can be changed to
meet regional preferences. See Language and Locale on
page 4–188.

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To Select a Time Zone


Click on the dropdown menu arrow at the end of the <System
Time Zone> field to reveal the list of choices.

Dropdown menu
arrow

Figure 4.198:Setting the Time Zone

Scroll up or down, as needed, and select the desired time zone.


Click <Save> when finished.

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Installation Section 4

Sample Bar Code


NOTE: The following procedure can also be performed
by customers with administrator access.
The m2000sp can be configured to read several different types
of sample bar code labels. These include the following
symbologies:
Table 4.7: Bar Code Symbologies

Symbology Editable Parameters


Interleaved 2 of 5 • Length (2-20 characters bar code
length)
• Use and transmission of Checksum
Code 39 Use and transmission of Checksum
UPCA Use and transmission of Checksum
Codabar • Use and transmission of Checksum
• Use and transmission of Start/Stop
characters
Code 128 Not editable
Code 93 Not editable

To configure a bar code:


1. Select <Settings> from the <System> menu. Then,
highlight <Sample Bar code> and choose <Configure>
from the <Setting Tasks> menu.
The <Bar Code Configuration> screen displays.

Figure 4.199:<Bar Code Configuration> Screen

2. Configure the length (Interleaved 2 of 5, 2 - 20 even) by


editing the number in the length column.
3. Configure the Checksum by using the dropdown menu
and selecting:
• Off (no Checksums)

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• On with transmit (Checksum in bar code transmitted,


or sent, to SCC for display)
• On without transmit (Checksum in bar code but not
sent to SCC)

Figure 4.200:Bar Code Configuration Checksum

NOTE: If Checksum for UPCA is set to <Off>, the


system will ignore this setting.
4. Configure the Start/Stop by using the dropdown menu
and selecting:
• Off
• On with transmit (start and stop characters used in
bar code and sent to SCC for display)
• On without transmit (start and stop characters used in
bar code but not sent to SCC for display)

Figure 4.201:Bar Code Configuration Start/Stop

NOTE: Codabar start/stop characters are always on.


“Off” is the same as “on without transmit.”

5. When configuration is complete, select <Save>.

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Installation Section 4

Sample Racks and Tubes


Sample tube size and style can be configured by selecting
<Sample Rack and Tube> from the <System> menu, and
choosing <Configure> from the <Setting Tasks> menu. Refer
to Sample Rack and Tube Configuration on page 4–149.

Run Checks
NOTE: The following procedure can also be performed
by customers with administrator access.
FSEs can enable or disable m2000sp run checks by selecting
<Settings> from the <System> menu, highlighting <Run
Checks>, and choosing <Configure> from the <Setting
Tasks> menu.

Figure 4.202: <Run Checks Configuration> Display

These checks are used to verify the correct placement of racks,


plates, and carriers, and the volume of sample preparation
reagents prior to beginning a run.
IMPORTANT: If these checks are disabled during
! installation, be sure to re-enable them before leaving
unless the customer specifically asks that they remain
disabled.

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Section 4 System Settings

FSEs can enable or disable the following Sample Extraction


checks:
• <Check for sufficient volume of Sample Preparation
Reagents>
Checks the minimum and/or maximum required fill
volume of each reagent vessel, as configured by the
application specification that is running and the number
of samples being processed. If there is too little or too
much volume, the fill volume must be corrected prior to
continuing with the run.
• <Check for 1 mL Subsystem Carriers in correct
positions in the 1 mL Subsystem>
Checks the placement of one or both 1 mL Subsystem
carriers and all empty locations. Carriers must be in the
correct locations prior to continuing the run (refer to the
<Worktable Setup> screen).
• <Check for the presence of Deep Well Plate on the
Output Deck>
Checks for the presence of the deep well plate on the
Output Deck. A warning is given if the check fails. The
user must then manually verify the plate is in position.
• <Check that Shelf Location 1 does not contain a
Disposable Tip Rack>
Checks that no DiTi rack is present in shelf location 1.
This shelf must be empty before the run can proceed.

Master Mix Addition check options are as follows:


• <Check for the presence of Deep Well Plate on the
Output Deck>
Checks for the presence of the deep well plate on the
Output Deck. A warning is given if the check fails. The
user must then manually verify the plate is in position.
• <Check for the presence of PCR Plate on the Output
Deck>
Checks for the presence of the PCR plate on the Output
Deck. A warning is given if the check fails. The user must
then manually verify the plate is in position.

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Installation Section 4

• <Check that Shelf Location 1 does not contain a


Disposable Tip Rack>
Checks that no DiTi rack is present in shelf location 1.
This shelf must be empty before the run can proceed.

When checks are disabled a message is sent to the Message Log


indicating such.

Signal Tower
NOTE: The following procedure can also be performed
by customers with administrator access.
The <Signal Tower> screen appears as an option on the
<Settings> menu for hooded instruments only.
The signal tower, which includes a red, a yellow, and a green
lamp, is designed to light up in a unique way for each of the
following five states:
• <Idle>: the instrument is ready
• <Running>: a run is in progress
• <Paused>: an instrument function was paused
• <Run Completed Successfully>: the run has finished
• <Run Failure>: the run has failed

The default signal tower configuration (i.e., what color


signifies what state) can be changed by doing the following:
1. Select <Signal Tower> from the <System Settings> menu,
and then select <Configure> from the <System Tasks>
menu.

2. The <Signal Tower Configuration> screen is divided into


five grids representing each of the five states listed above.

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Section 4 System Settings

Each grid is divided into three color menus featuring the


following choices:
• <Off>: that lamp color will not light up in the selected
state
• <On>: that lamp color will light up in the selected
state
• <Blinking>: that lamp color will blink on and off in
the selected state
To make changes, click on the choice desired for each
color.
3. To see the signal tower display the new setting, click the
<Test> button for that state.
4. Select <Save> after all changes have been made.

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Installation Section 4

Language and Locale


NOTE: The following procedure can also be performed
by customers with administrator access.

Figure 4.203:<Language and Locale> screen

On the <Language and Locale> screen, the following settings


can be changed to meet regional preferences:
NOTE: The <Language> field cannot be changed.

• <Input Language>, which determines what regional


keyboard layout will be used with the system
• <Document Language>, which determines what
language the on-line documentation (e.g., the m2000sp
Operations Manual) will display in when accessed
through the <Help> menu
• <Locale>, which determines:
– how numeric data are presented in reports and screens
– how the date and time is displayed and entered for
expiration dates

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To make changes, first select <Settings> from the <System>


menu, highlight <Language and Locale>, and choose
<Configure> from the <Setting Tasks> menu.
IMPORTANT: Changes to the Language and Locale
! affect the keyboard and display within the m2000sp
application only. In S&S, Explorer, etc., the keyboard
and displays will act and appear as they would right out
of the box regardless of what Language and Locale
setting is chosen.

To Change <Input Language>


Changing the keyboard preference is a two-part process:
• Changing the preference on the SCC
• Installing the new keyboard

Changing the Preference on the SCC


1. On the <Language and Locale> page, go to the <Input
Language> field and click on the dropdown menu to
select a keyboard layout preference.

Figure 4.204:<Input Language> Field

2. Select <Save>.

Installing the New Keyboard


1. Unplug the original keyboard from the back of the
computer.
2. Plug the new keyboard into the back of the computer, but
be careful not to disturb the other cables.
NOTE: If a handheld bar code reader is installed,
plug it into the new keyboard.
NOTE: Contact your Area Abbott Customer
Service for a list of supported keyboards.

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Installation Section 4

To Change <Document Language>


1. If you have not already done so, install the applicable
translated document versions found on the GSS website’s
Software Updates page.
2. On the <Language and Locale> page, go to the
<Document Language> field and click on the dropdown
menu to select a language preference for the on-line
documentation, such as the m2000sp Operations
Manual.

Figure 4.205:<Document Language> Field

3. Select <Save>.

To Change <Locale>
1. On the <Language and Locale> page, go to the <Locale>
field and click on the dropdown menu to select a regional
display preference.
2. Scroll down the list and make the selection that best fits
your preferences.

Figure 4.206:Choose a preference

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Section 4 System Settings

3. The <Examples> section below the dropdown menu will


show examples of how dates, times, and numerical data
will be depicted in the m2000sp Software.

Figure 4.207:Examples of New Formats

If these new formats are acceptable, and if all other


changes have been made to the settings on this screen,
select <Save>.
NOTE: Changes made to the <Locale> setting
require a system restart to implement. It is best to
make all desired changes to the other settings on
the <Language and Locale> screen before
selecting <Save> here.
4. A message displays explaining that the change requires a
system restart. Select <Yes>.
After the system restarts, the date and time will be
displayed in the new format.

Figure 4.208:Reformatted Date and Time Display

The on-line documents, found under the <Help>,


<Online Documentation> menu, will now appear in the
language selected if it has been loaded onto the system by
the FSE.

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Installation Section 4

Network Configuration
Refer to Network Configuration on page 4–168.

Automatic Export
NOTE: The following procedure can also be performed
by customers with administrator access.
If a network or crossover cable connection was configured
during installation, customers with Administrator access can
choose to have Test Order results exported automatically on
the <Automatic Export> page.
1. To enable or disable automatic exporting, select
<Settings> from the <System> menu, highlight
<Automatic Export>, and choose <Configure> from the
<Setting Tasks> menu.

Figure 4.209:<Automatic Export Configuration> screen

2. On the <Automatic Export Configuration> screen, select


one or all of the following export options:
• <Sample Extraction Open Mode> for Sample
Extraction open mode; deep well plate data will be
transferred at the end of a successful open-mode
Sample Extraction run
• <Master Mix Addition Open Mode> for reagent
addition open mode; PCR plate data will be
transferred after a successful Master Mix Addition run
• <Master Mix Addition Closed Mode> for reagent
addition closed mode; PCR plate data will be
transferred after a successful Master Mix Addition run
3. When finished, select <Save>.

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Section 4 System Settings

NOTE: Results can still be manually transferred via CD


at any time.

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Installation Section 4

NOTES

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Section 5: Maintenance and Diagnostic Procedures

SECTION 5:
MAINTENANCE AND
DIAGNOSTIC
PROCEDURES

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Maintenance and Diagnostic Procedures Section 5

NOTES

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2000942-103—September 2007
Section 5 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Pausing/Resuming/Stopping a Process . . . . . . . . . . . . . . 5-6
Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Flushes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Simple Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Extensive Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
LiHa Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
LiHa DiTi Location Test . . . . . . . . . . . . . . . . . . . . . . . . 5-12
LiHa Sample Tube Check . . . . . . . . . . . . . . . . . . . . . . . 5-14
LiHa Output Deck Test . . . . . . . . . . . . . . . . . . . . . . . . . 5-16
1 mL Subsystem Alignment Check . . . . . . . . . . . . . . . . 5-19
Dispense Test 50 µL, Visual . . . . . . . . . . . . . . . . . . . . . 5-21
Dispense Test 50 µL, Gravimetric . . . . . . . . . . . . . . . . . 5-23
Dispense Test 800 µL, Visual . . . . . . . . . . . . . . . . . . . . 5-24
Dispense Test 800 µL, Gravimetric . . . . . . . . . . . . . . . . 5-26
RoMa Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-27
RoMa DiTi Rack Test . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-27
RoMa DiTi Shelf Test . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-29
RoMa 1 mL Carrier Test . . . . . . . . . . . . . . . . . . . . . . . . . 5-30
System Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-32
LiHa Output Test - Sample Extraction . . . . . . . . . . . . . 5-32
LiHa Output Test - Master Mix . . . . . . . . . . . . . . . . . . . 5-35
Sample Extraction Test. . . . . . . . . . . . . . . . . . . . . . . . . . 5-37
Z-Calibration Check – Sample Extraction . . . . . . . . . . 5-40
Z-Calibration Check – Master Mix . . . . . . . . . . . . . . . . 5-45
RNA Simulation Test – Sample Extraction . . . . . . . . . . 5-47
RNA Simulation Test – Master Mix . . . . . . . . . . . . . . . . 5-50
Other Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-52
Chiller and Heater Temperature Check . . . . . . . . . . . . 5-52
Ethanol LLS Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-54
LiHa Sample Volume Checks . . . . . . . . . . . . . . . . . . . . 5-56

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Maintenance and Diagnostic Procedures Section 5

Purpose of This Section


This section provides information for using the Maintenance
and Diagnostics (M&D) functions within the m2000sp
software, including an overview of each function and
directions for use.
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
WARNING: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.

WARNING: Class II Laser Product. Identifies a Class 2


laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing of the bar code laser beam or
reflections of the beam.
WARNING: Electrical Shock Hazard: Possible danger
from electrical shock. Switch off the main power and
disconnect the main power connection of the
instrument before replacing fuses, printed circuit
boards, major sub-assemblies, etc. Replace only fuses
that are externally accessible and labeled. Only use
replacement fuses of the specified type and electrical
rating.

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Section 5 Overview

Overview
M&D procedures for use in troubleshooting and confirmation
of setup by FSEs are found under one of the following two
menus:
• Maintenance Procedures, found under the System menu
• Sample Extraction, found under the Orders menu
The following table indicates which M&D procedures are
found under which menu.
Table 5.1: M&D Menu Locations

Menu Protocol Name


• m2000sp Simple Flush
• m2000sp Extensive Flush
• m2000sp Instrument Daily Maintenance
• LiHa DiTi Location Test
Maintenance Procedures
• RoMa DiTi Rack Test
• RoMa DiTi Shelf Test
• RoMa 1 mL Carrier Test
• Chiller and Heater Temperature Check
• LiHa Output Deck Test (Alignment)
• 1 mL Subsystem Alignment Check
• 50 µL Dispense Tests
• 800 µL Dispense Tests
• LiHa Sample Tube Check
• LiHa Output Test
Sample Extraction
• Sample Extraction Test
• Pierceable Cap Rack Test
• Diagnostic - Z-Calibration Check
• RNA Simulation Tests
• Ethanol LLS Check
• LiHa Sample Volume Checks

When a problem is discovered during any M&D procedure, try


recalibrating the location. (Refer to LiHa Calibrations on page
4–134 and RoMa Calibrations on page 4–157 of Section 4,
Installation.)
If that does not solve the problem, proceed to Section 7,
Troubleshooting, and find the section relevant to the problem.

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Maintenance and Diagnostic Procedures Section 5

Pausing/Resuming/Stopping a Process
To pause a process, select <Pause> or <Immediate Pause>
from the left-hand menu. At the prompt, select <Yes>.

Figure 5.1: Pause a Process

The <Pause> option stops an in-process application run as


soon as the instrument reaches the next pipetting step
(homing the LiHa and RoMa), allowing the user to update the
disposable tip status, if necessary. The <Immediate Pause>
option instantly stops the instrument, regardless of what
activity is being performed or what stage the run is in.
To resume a process, select <Resume> from the left-hand
menu. At the prompt, select <Yes>.

Figure 5.2: Resume a Process

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Section 5 Overview

To stop a process, select <Stop Process> from the left-hand


menu. At the prompt, select <Yes>.

Figure 5.3: Stop a Process

At the stop request prompt, select <Close>.

Figure 5.4: Stop Request Prompt

At the <Process Aborted> prompt, select <Close>.

Figure 5.5: Process Aborted Prompt

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Maintenance and Diagnostic Procedures Section 5

At the <Process Completed> prompt, select <Close>.

Figure 5.6: Process Completed Prompt

At the prompt, select <Yes>.

Figure 5.7: Close the Process

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Section 5 Daily Maintenance

Daily Maintenance

There are four process steps in Daily Maintenance.

• Tighten the liquid handling components


• Clean the m2000sp components
• Check containers in the cabinet
• System flush

See the m2000sp Operations Manual, Section 9, Service and


Maintenance, for more information.

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Maintenance and Diagnostic Procedures Section 5

Flushes
Simple Flush
The <Simple Flush> purge might be required when:
• Isolated bubbles are found in the tubing between 3-way
valves and DiTi cones
• System sits idle for a few hours between runs
• Any LiHa component between the 3-way valve and DiTi
cone has been reassembled for replacement, calibration, or
check

1. To perform the <Simple Flush>, close the safety door.


2. When the system is in the READY state, from the menu
select <System> then <Maintenance Procedures>.
3. Select <m2000sp Simple Flush> from the <Maintenance
Procedures> section.
4. Select <Set up Run> from the <Maintenance Tasks>
menu.
5. The <Maintenance Procedure: Run Start> screen
displays. Select <Start>.
6. The system will perform the flush with the use of the Fast
Wash (FaWa) pump and syringes.
7. When prompted that the procedure has completed
successfully, select <Close Process> from the <Process
Tasks> menu.

Extensive Flush
The <Extensive Flush> purge might be required when:
• System is first set up
• When system fluid is refilled (outside of Daily
Maintenance)
• Any component within the fluid handling system has
been reassembled for maintenance, replacement,
calibration or check (e.g., replacing the system liquid)
• Special flush is performed using a cleaning solution

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Section 5 Flushes

1. To perform the <Extensive Flush>, close the safety door


2. When the system is in the READY state, from the menu
select <System> then <Maintenance Procedures>
3. Select <m2000sp Extensive Flush> from the
<Maintenance Procedures> section
4. Select <Set Up Run> from the <Maintenance Tasks>
menu
5. The <Maintenance Procedure: Run Start> screen
displays. Select <Start>
6. The system will perform the flush with the use of the
FaWa pump and syringes
7. When prompted that the procedure has completed
successfully, select <Close Process> from the <Process
Tasks> menu

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Maintenance and Diagnostic Procedures Section 5

LiHa Checks
LiHa DiTi Location Test

Purpose
During this test, the LiHa will pick up and eject tips, allowing
the user to visually check the alignment of the DiTi cones with
the DiTis underneath.

Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips in each position
After the procedure:
• Adjust X and Y alignment in the DiTi racks and reuse racks
as needed

Procedure
1. Perform the following steps to set up the worktable:
• Load two 1000 µL DiTi racks with empty DiTi trays on
DiTi rack carrier 1
• Load two 200 µL DiTi racks with empty DiTi trays on
DiTi rack carrier 2
• Load empty DiTi trays and deep well plates in the DiTi
reuse racks on both DiTi rack carriers
• Fill column 1 and column 12 of all six DiTi trays with
DiTis in appropriate sizes, as shown below.

Figure 5.8: LiHa DiTi Location Test DiTi Carrier Setup

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Section 5 LiHa Checks

2. Select <System> then <Maintenance Procedures>, or


select the Maintenance Procedure icon.
3. Select <LiHa DiTi Location Test> from the
<Maintenance Procedures> screen and select <Set Up
Run> from the <Maintenance Tasks> menu.
4. When the worktable has been loaded, close the door and
select <Start> to begin the test.
5. The LiHa will stake and eject DiTis from the selected
columns. After doing this at each column, the LiHa will
pause briefly above the DiTis in the tray. Check the
alignment of the DiTi cones with the DiTis underneath.
When the LiHa has completed its check of a specific tray,
it will stop above it, and a message indicating this will
appear. Select <Close> to close the message box and then
select <Resume> from the <Process Tasks> menu.
At each of the DiTi reuse racks, the LiHa will attempt to
pick up DiTis twice at column 6, with a pause at each
attempt. Check the alignment of the DiTi cones in the
empty columns.
6. If a problem is observed, select <Stop Process> to abort the
test. Otherwise, select <Resume> to continue the test.
7. The test will end automatically after checking all six DiTi
racks.
8. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
9. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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Maintenance and Diagnostic Procedures Section 5

LiHa Sample Tube Check

Purpose
During this test, the DiTi locations at the input sample tubes
will be visually verified.

Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips in each position: the
tips should be more or less centered in all tubes, with some
variability allowed as tubes sit differently in the racks
After the procedure:
• Adjust X and Y alignment in the input sample locations as
needed

Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Run all sample tube types being used by the customer,
but ensure that the sizes are not mixed on a rack (i.e.,
all 13 mm on one rack; all custom tubes on another
rack) and that the tube type matches the rack type
• Run all sample rack types being used by the customer
(13, 16, custom, or transport [pierceable])
• Run 16 tubes on each rack to check for run out from
front to back on the system
• Load reagent vessel carriers on the worktable (no bar
codes are needed)
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- LiHa Sample Tube Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.

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Section 5 LiHa Checks

6. Enter arbitrary numbers for the <Sample ID> when


prompted. Select <Next>.
At the <Sample Extraction: Worktable Setup> screen,
ignore all instructions and select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there is a full 1000 µL DiTi rack at
location 1 of DiTi rack carrier 1. Update the status, if
necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
9. Tips will pause briefly in the sample tubes for aspiration.
Observe alignment during this time, or select <Immediate
Pause> from the <Sample Extraction Tasks> menu for an
extended observation. The tips then dive to the bottom of
the tubes to check Z-calibration.
The check is considered successful if all DiTis are aligned
in the center area of the sample tubes and no drive no
load errors are encountered on the dive.
10. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
11. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

LiHa Output Deck Test

Purpose
During this test, the DiTi X-Y alignment at the assay reagent
bottles, master mix tube, deep well plate, and PCR plate on the
Output Deck will be visually verified.

Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips at all Output Deck
locations
After the procedure:
• Adjust X and Y alignment in the Output Deck locations as
needed

Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load an input sample rack with 8 tubes
• Load 8 reaction vessels in a 1 mL Subsystem carrier,
and place the carrier in the room temperature zone
• Load one reagent vessel containing a minimum of 20
mL of tap water or buffer solution into reagent vessel
position 6 on reagent vessel carrier 2 (the vessel
closest to the safety door) and place on the worktable
(no bar codes are needed)
• Load a clean, empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi carrier 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Load one empty reagent pack at position 1 (no bar
code needed)
• Load a clean master mix tube at position 1 on the
master mix tube holder
• Load a deep well plate onto the Output Deck
• Load a clean PCR plate on the chiller

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2000942-103—September 2007
Section 5 LiHa Checks

2. Select <Orders>, <Sample Extraction>, then <Diagnostic


- LiHa Output Deck Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next>. The <Sample Extraction: Assay Details>
screen displays.
5. In the <Actual Concentration> fields, type in the same
numbers as those listed under <Target Concentration>.
Select <Next> at the lower-right corner of the screen.
6. Close the door and select <Scan>. Proceed with the run as
with a typical Sample Extraction run.
7. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
8. When the <Sample Extraction: Assay Assignment>
screen displays, select <Next>. Then, ignore the <Sample
Extraction: Warnings> screen and select <Next> again.
9. At the <Sample Extraction: Worktable Setup> screen,
verify the 1 mL Subsystem carrier is in the room
temperature zone and that it contains 8 reaction vessels,
then select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there is a full 1000 µL DiTi rack at
location 1 of DiTi rack carrier 1 and a full 200 µL DiTi
rack at location 4 of DiTi rack carrier 2. Update the status,
if necessary, and select <Next>.
10. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
11. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>. A modified
sample preparation procedure will be performed.
12. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.

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Maintenance and Diagnostic Procedures Section 5

13. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
14. Select <Orders>, <Master Mix Addition>, then in the
<Select a Plate> field, highlight the plate that was just
run. (This plate can be used repeatedly.)
15. Select <Set Up Run> from the <Master Mix Addition
Tasks> menu and perform the protocol as a typical Master
Mix Addition run.
16. Select <Next> on the <Master Mix Addition: Plate
Details> screen.
17. At the <Master Mix Addition: Worktable Setup> screen,
enter arbitrary numbers for the <PCR Plate Name> and
select <Next>. Ignore all on-screen instructions.
When the <Master Mix Addition: Disposable Tip
Status> screen appears, select <Next>.
18. On the <Master Mix Addition: Assay Specific Reagent
Scan> screen, select <Scan>, then <Next>.
19. Verify the master mix tube is in the correct location as
indicated on the <Master Mix Addition: Run Start>
screen and select <Start>.
NOTE: If a problem occurs and it becomes
necessary to rerun this test, it will NOT be
necessary to repeat the Sample Extraction portion.
20. The LiHa travels to various locations on the Output Deck,
diving into each location and pausing briefly. As it does,
visually ensure the DiTis are properly centered at each
location.
NOTE: If more time for visual inspection is
needed, select <Immediate Pause> from the
<Sample Extraction Tasks> menu while the LiHa
is paused. The safety door may be opened at this
time. Select <Resume> when the inspection is
complete.
21. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
22. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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2000942-103—September 2007
Section 5 LiHa Checks

1 mL Subsystem Alignment Check

Purpose
During this test, the DiTi X-Y alignment at the reaction vessels
on the 1 mL Subsystem will be visually verified.

Actions
As you run this procedure, be sure to:
• Check X and Y alignment of the tips in each position,
verifying they are more or less centered throughout
• Check for positioning and alignment of the 1 mL
Subsystem carriers, ensuring they are set into position
evenly and with minimal deflection
After the procedure:
• Adjust calibration positions as needed

Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load input sample racks with a minimum of 16 tubes
• Load the same number of reaction vessels in a 1 mL
Subsystem carrier, beginning at position 1, and place
the carrier in the room temperature zone
• Load a clean, empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi carrier 1
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
– 1 mL Subsystem Alignment Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.

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Maintenance and Diagnostic Procedures Section 5

At the <Sample Extraction: Worktable Setup> screen,


verify the 1 mL Subsystem carrier is in the room
temperature zone and that it contains the right number
of reaction vessels in the positions indicated on the
screen, then select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there is a full 1000 µL DiTi rack at
location 1 of DiTi rack carrier 1. Update the status, if
necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
9. The LiHa stakes tips, spreads, and dives into the reaction
vessels in the 1 mL Subsystem, and then pauses for 10
seconds. Visually ensure each DiTi is properly centered
within a reaction vessel.
NOTE: If more time for visual inspection is
needed, select <Immediate Pause> from the
<Sample Extraction Tasks> menu while the LiHa
is paused. The safety door may be opened at this
time. Select <Resume> when the inspection is
complete.
The RoMa will move the carrier to every position in the
1 mL Subsystem. At each location, the LiHa will repeat
the process of entering and pausing within the reaction
vessels. Ensure the DiTis are centered at every location.
10. When the check is finished, select <Close>, then <Close>
again to clear the message boxes.
11. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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Section 5 LiHa Checks

Dispense Test 50 µL, Visual

Purpose
This small-volume test verifies the accuracy of fluidic
pipetting.

Actions
At the end of this procedure, be sure to:
• Visually inspect the RVs for a consistent level of fluid
• If inconsistencies are found, check the system fluidics for
leaks

Procedure
1. Set up the worktable as follows:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load input sample racks with 48 tubes, starting with
tube position 1
• Load the 1 mL Subsystem carrier with 48 clean
reaction vessels, beginning at position 1, and place
the carrier in the room temperature zone
• Load both reagent vessel carriers on the worktable (no
bar codes are needed)
• Fill the reagent vessel at location 6 (the vessel closest
to the safety door) with a minimum of 20 mL of tap
water or buffer solution.
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- 50 µL Dispense Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

At the <Sample Extraction: Worktable Setup> screen,


verify the 1 mL Subsystem carrier is in the room
temperature zone and that it contains the right number
of reaction vessels in the positions indicated on the
screen, then select <Next>.
The instrument then checks the 1 mL Subsystem setup.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there is a full 1000 µL DiTi rack at
location 1 of DiTi rack carrier 1. Update the status, if
necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
9. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
10. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
11. When the door is unlocked, visually inspect the reaction
vessels in the 1 mL Subsystem for consistency of dispensed
volumes.

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Section 5 LiHa Checks

Dispense Test 50 µL, Gravimetric

Purpose
This small-volume test verifies the accuracy of fluidic
pipetting.

Actions
If inconsistencies are found at the end of this procedure, check
the system fluidics for leaks.

Procedure
All steps should be performed as outlined in the Dispense Test
50 µL, Visual, with the following changes:
1. Weigh each empty reaction vessel on a balance (accurate
to 0.5 milligrams or better) before loading it onto the 1 mL
Subsystem carrier during worktable setup. Number each
tube.
2. Weigh each reaction vessel from the 1 mL Subsystem
carrier at the end of the run using the same balance.
3. Calculate the volume transferred during the run by
subtracting the two values and then dividing that result by
the density of water (.000998 g/µL).
4. The gravimetric test passes if the mean value of the
calculated volume transferred is between 47.5 µL and 52.5
µL.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

Dispense Test 800 µL, Visual

Purpose
This large-volume test verifies the accuracy of fluidic pipetting.

Actions
At the end of this procedure, be sure to:
• Visually inspect the RVs for a consistent level of fluid
• If inconsistencies are found, check the system fluidics for
leaks

Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load input sample racks with 48 tubes, starting with
tube position 1
• Load the 1 mL Subsystem carrier with 48 clean
reaction vessels, beginning at position 1, and place
the carrier in the room temperature zone
• Load reagent vessel carriers on the worktable (no bar
codes are needed)
• Fill the reagent vessel at location 6 (the vessel closest
to the safety door) with a minimum of 50 mL of tap
water or buffer solution
2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- 800 µL Dispense Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Close the door and select <Scan>. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.

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Section 5 LiHa Checks

At the <Sample Extraction: Worktable Setup> screen,


verify the 1 mL Subsystem carrier is in the room
temperature zone and that it contains the right number
of reaction vessels in the positions indicated on the
screen, then select <Next>.
The instrument then checks the 1 mL Subsystem setup.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there is a full 1000 µL DiTi rack at
location 1 of DiTi rack carrier 1. Update the status, if
necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
9. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
10. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
11. When the door is unlocked, visually inspect the reaction
vessels in the 1 mL Subsystem for consistency of dispensed
volumes.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

Dispense Test 800 µL, Gravimetric

Purpose
This large-volume test verifies the accuracy of fluidic pipetting.

Actions
If inconsistencies are found at the end of this procedure, check
the system fluidics for leaks.

Procedure
All steps should be performed as outlined in the Dispense Test
800 µL, Visual, with the following changes:
1. Weigh each empty reaction vessel on a balance (accurate
to 0.5 milligrams or better) before loading it onto the 1 mL
Subsystem carrier during worktable setup. Number each
tube.
2. Weigh each reaction vessel from the 1 mL Subsystem
carrier at the end of the run using the same balance.
3. Calculate the volume transferred during the run by
subtracting the two values and then dividing that result by
the density of water (.000998 g/µL).
4. The gravimetric test passes if the mean value of the
calculated volume transferred is between 760 µL and
840 µL.

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Section 5 RoMa Checks

RoMa Checks
RoMa DiTi Rack Test

Purpose
During this test, the RoMa will move the DiTi racks between
the DiTi rack carrier and the DiTi rack shelf.

Actions
As you run this procedure, be sure to:
• Check for positioning and alignment of the DiTi racks in
each position
• Verify that all movements are smooth, with minimal
contact between the racks and the surfaces
After the procedure:
• Adjust calibrations of the DiTi racks as needed

Procedure
1. Perform the following steps to set up the worktable:
• Load two 1000 µL DiTi racks on DiTi rack carrier 1 in
positions 1 and 2
• Load two 200 µL DiTi racks on DiTi rack carrier 2 in
positions 1 and 2
• Load 1000 µL DiTi racks in positions 2 through 8 on
the shelf
NOTE: For the purpose of this test, it does not
matter whether DiTis are present in the racks.

2. Select <System> then <Maintenance Procedures> or


select the Maintenance Procedure icon.
3. Select <RoMa DiTi Rack Test> from the <Maintenance
Procedures> screen, and select <Set Up Run>.
4. Close the door and select <Start> to begin the test.
5. The RoMa will move the DiTi racks to all positions.
Observe the DiTi rack movements and record any
problems found. The RoMa will return to the home
position after each step.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

6. If a problem is observed, select <Stop Process> to abort the


test.
7. The test will end automatically after checking all
locations.
8. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
9. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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Section 5 RoMa Checks

RoMa DiTi Shelf Test

Purpose
During this test, the RoMa will move a DiTi rack to each of the
DiTi rack shelf's eight positions.

Actions
As you run this procedure, be sure to:
• Check for positioning and alignment of the DiTi rack in
each shelf
• Verify that all movements are smooth, with minimal
contact between the racks and the surfaces
After the procedure:
• Adjust calibrations of the DiTi racks as needed

Procedure
1. Perform the following steps to set up the worktable:
• Load one 1000 µL DiTi rack in shelf location 1
• Remove all other DiTi racks from the shelf
2. Select <System> then <Maintenance Procedures> or
select the Maintenance Procedure icon.
3. Select <RoMa DiTi Shelf Test> from the <Maintenance
Procedures> screen, and select <Set Up Run>.
4. Close the door and select <Start> to begin the test.
5. The RoMa will move the DiTi rack from shelf to shelf in
sequential order. At the end of each step, the RoMa will
return home.
6. If a problem is observed, select <Stop Process> to abort the
test.
7. The test will end automatically after checking all
locations.
8. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
9. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

RoMa 1 mL Carrier Test

Purpose
During this test, the RoMa will move two 1 mL Subsystem
carriers to all positions within the 1 mL Subsystem.

Actions
As you run this procedure, be sure to:
• Check for positioning and alignment of the 1 mL
Subsystem carriers in each position, ensuring they set into
position evenly and with minimal deflection of the RVs
• Check for interleaving of the carriers; no contact should
occur
After the procedure:
• Adjust calibration of the 1 mL Subsystem carriers as
needed

Procedure
1. Perform the following steps to set up the worktable:
• Load 96 empty reaction vessels into two 1 mL
Subsystem carriers (48 vessels per carrier)
• Lock the reaction vessels in place with the 1 mL
Subsystem carrier cover
• Place the first 1 mL Subsystem carrier in heater zone 1
and the second carrier in heater zone 2
2. Select <System> then <Maintenance Procedures> or
select the Maintenance Procedure icon.
3. Select <RoMa 1 mL Carrier Test> from the <Maintenance
Procedures> screen, and select <Set Up Run>.
4. Close the door and select <Start> to begin the test.
5. The RoMa will move the carriers to all positions on the 1
mL Subsystem. Observe the carrier movements and record
any problems found, looking for carrier placement
misalignment.
6. If a problem is observed, select <Stop Process> to abort the
test.
7. The test will end automatically after checking all
locations.

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Section 5 RoMa Checks

8. When the process is completed, select <Close>, then


<Close> again to clear the message boxes.
9. Select <Close Process> from the <Process Tasks> menu to
close the test, and confirm with <Yes>.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

System Checks
LiHa Output Test - Sample Extraction

Purpose
This is the first portion of a buffer run procedure that enables
the user to evaluate m2000sp performance through a Sample
Extraction sequence, including transfer of eluate from the
reaction vessels to the deep well plate in the Output Deck. The
master mix process will be completed in the second portion of
the run.
NOTE: Two 995 reagent bar codes and reagent packs
will be needed for the master mix portion of this test.

Actions
At the end of the procedure, be sure to:
• Verify that no errors occurred (if any occur, the test has
failed)
• Examine the Process Logs for the minimum level sense
numbers:
– verify that the minimum level sense at MagRest minus
the calibrated bottom is greater than or equal to 7
– if this is not the case, check the calibration or the
system hardware

Procedure
1. Perform the following steps to set up the worktable:
• Load two full racks of 1000 µL DiTis on DiTi carrier 1
in locations 1 and 2
• Load input sample racks with 48 tubes
• Load 48 reaction vessels in a 1 mL Subsystem carrier,
and place the carrier in the room temperature zone
• Load one reagent vessel containing a minimum of 20
mL of tap water or buffer solution into reagent vessel
position 6 on reagent vessel carrier 2 (the vessel
closest to the safety door), and place the carrier on the
worktable (no bar codes are needed)
• Load a clean, empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi carrier 1

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Section 5 System Checks

• Load a clean deep well plate onto the Output Deck


2. Select <Orders>, <Sample Extraction>, then <Diagnostic
- LiHa Output Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next>. The <Sample Extraction: Assay Details>
screen displays.
5. In the <Actual Concentration> fields, type in the same
numbers as those listed under <Target Concentration>.
Select <Next> at the lower-right corner of the screen.
6. Close the door and select <Scan>. Proceed with the run as
with a typical Sample Extraction run.
7. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
8. When the <Sample Extraction: Assay Assignment>
screen displays, select <Next>. Then, ignore the <Sample
Extraction: Warnings> screen and select <Next> again.
9. At the <Sample Extraction: Worktable Setup> screen,
verify the 1 mL Subsystem carrier is in the room
temperature zone and that it contains the right number of
reaction vessels in the positions indicated on the screen,
then select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there are two full 1000 µL DiTi
racks on DiTi rack carrier 1. Update the status, if
necessary, and select <Next>.
10. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
11. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
12. Observe the action of the instrument at all process steps.

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Maintenance and Diagnostic Procedures Section 5

13. When the process is completed, select <Close>, then


<Close> again to clear the message boxes.
14. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
15. After the door is unlocked, visually inspect the 1 mL
carrier and the deep well plate. There should be very little
residual fluid in each reaction vessel in the 1 mL carrier.
There should be a uniform level of fluid in each of the first
48 wells in the deep well plate.
16. After the Sample Extraction inspection has been
completed, the Master Mix Addition portion of this
diagnostic test can be performed.

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Section 5 System Checks

LiHa Output Test - Master Mix

Purpose
This is the second portion of the buffer run procedure that
begins with the LiHa Output Test - Sample Extraction. This test
will take the plate created in the previous test and complete
the Master Mix Addition process.
NOTE: Two 995 reagent bar codes and reagent packs
will be needed for the master mix portion of this test.

Actions
As you run this procedure, be sure to:
• Pause after the master mix to PCR plate transfer to check
that all wells have an equal amount of fluid; if there is
inconsistency, check the system fluidics for leaks
After the procedure:
• Verify that all wells still have an equal amount of fluid

Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Beginning at position 1, load two reagent packs (with
reagent bar code 995) and reagent vials filled with tap
water in the volumes specified below:
Table 5.2: Tap Water Volumes

Vial Position Target Fill Volume


1 0.40 mL
2 1.05 mL
3 0.14 mL

• Load a clean master mix tube at the position that


corresponds to the left-most reagent pack location on
the master mix tube holder

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Maintenance and Diagnostic Procedures Section 5

• Ensure that the deep well plate created in the previous


LiHa Output Test – Sample Extraction is located on
the Output Deck
• Load a clean PCR plate on the chiller
2. Select <Orders>, <Master Mix Addition>, then in the
<Select a Plate> field, select the plate that was run in the
first portion of the buffer run.
3. Select <Set Up Run> from the left-hand option menu.
4. Close the door and perform the protocol as a typical
Master Mix Addition run. Select <Next> on the <Master
Mix Addition: Plate Details> screen.
5. At the <Master Mix Addition: Worktable Setup> screen,
enter arbitrary numbers for the <PCR Plate Name> and
select <Next>. Ignore all on-screen instructions.
When the <Master Mix Addition: Disposable Tip
Status> screen appears, verify there is a full 1000 µL DiTi
rack in location 1, on DiTi rack carrier 1, and a full 200 µL
DiTi rack in location 4, on DiTi rack carrier 2. Update the
status, if necessary, and select <Next>.
6. On the <Master Mix Addition: Assay Specific Reagent
Scan> screen, select <Scan>, then <Next>.
7. Verify the master mix tube is in the correct location as
indicated on the <Master Mix Addition: Run Start>
screen and select <Start>.
8. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
9. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
10. When the door is unlocked, visually inspect the PCR plate.
The first 48 wells should be filled with about the same
amount of fluid.

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Section 5 System Checks

Sample Extraction Test

Purpose
During this procedure, a buffer run will be performed to
evaluate m2000sp performance through an entire Sample
Extraction sequence, except for eluate transfer from the
reaction vessels to the deep well plate.
NOTE: Reagent vessel RNA bar codes will be needed for
this test.

Actions
As you run this procedure, be sure to:
• Inspect the RVs following each liquid removal to verify
that all liquid was removed
• Check X and Y alignment of the tips—especially tip 3—in
each position, ensuring they are more or less centered in
all tubes
• Ensure that no water drops fall off the tips at the reagent
vessel locations during the system pause; if drops fall,
check the system fluidics for leaks
After the procedure:
• Adjust X and Y calibrations as needed

Procedure
1. Perform the following steps to set up the worktable:
• Load three full racks of 1000 µL DiTis on DiTi carrier
1, positions 1 and 2, and shelf position 2
• Load a clean empty DiTi tray and a clean deep well
plate onto the DiTi Reuse rack on DiTi Carrier 1
• Load 13 mm sample racks with 48 reaction vessels,
filled with a minimum of 1 mL of tap water
• Load a 1 mL Subsystem carrier with 48 reaction vessels
and place the carrier in heater zone 1

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Maintenance and Diagnostic Procedures Section 5

• Load the reagent vessels, filled with tap water at the


volumes indicated below, onto reagent vessel carriers
1 and 2 and load onto the worktable (RNA bar codes
are needed on the reagent vessels)
Table 5.3: Reagent Vessel Volumes

Location Location Label Minimum Fill Volume


1 mLysis 45 mL
3 mMicroparticles 15 mL
4 mWash 1 45 mL
5 mWash 2 45 mL

2. Select <Orders>, <Sample Extraction>, then <Diagnostic


- Sample Extraction Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Close the door, select <Scan>, and proceed.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
At the <Sample Extraction: Worktable Setup> screen,
verify the 1 mL Subsystem carrier is in heater zone 1 and
that it contains the right number of reaction vessels in
the positions indicated on the screen, then select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there are full racks of 1000 µL DiTis
on DiTi carrier 1, positions 1 and 2, and in shelf position
2. Update the status, if necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.

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Section 5 System Checks

9. Look for droplets above reagent vessels 4 and 5 during the


pause (10 seconds). If they are detached during the pause,
leakage is indicated.
10. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
11. Select <Close Process> from the <Process Tasks> menu to
close the test, and confirm with <Yes>.
12. When the door is unlocked, visually inspect the 1 mL
Subsystem carrier. All reaction vessels in the carrier should
have minimal residual liquid (< 10 µL).

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Maintenance and Diagnostic Procedures Section 5

Z-Calibration Check – Sample Extraction

Purpose
This is the first portion of a buffer run procedure. During this
test, Z-calibration at the input sample tubes, heater zones 1
and 2, magnetic rest, and the deep well plate locations will be
verified.

Actions
At the end of the procedure, be sure to:
• Verify that no errors occurred (if any occur, see
troubleshooting table below)
• Inspect the 1 mL Subsystem carrier to ensure that all liquid
was removed from the RVs
• Ensure the first 48 wells in the deep well plate contain an
equal amount of fluid: if there is inconsistency, check the
system fluidics for leaks
Table 5.4: Z-Calibration Troubleshooting

Location Error Reason

Heater zone 1 Drive no load (3110) Calibration too low


Heater zone 1 Specified submerge position for the liquid Calibration too high
volume exceeds the calibrated Z-bottom (3152)
Sample aspiration Drive no load (3110) Calibration too low
Sample aspiration Insufficient volume for aspiration (3153) Calibration too high
Magnetic rest Specified submerge position for the liquid Calibration too high
volume exceeds the calibrated Z-bottom (3152)
Magnetic rest More liquid than expected (3131) Calibration too low
Heater zone 2 Drive no load (3110) Calibration too low
Heater zone 2 Specified submerge position for the liquid Calibration too high
volume exceeds the calibrated Z-bottom (3152)
Deep well plate Specified submerge position for the liquid Calibration too high
volume exceeds the calibrated Z-bottom (3152)

• For errors found during this check, perform the following


sequence of steps:

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Section 5 System Checks

1. Check the sp calibration and adjust as needed.


– If a calibration adjustment is made, rerun the
Z-Calibration Test. If the test passes, proceed to the
master mix portion of this check.
– For 1 mL Subsystem issues, if the calibration did
not require adjustment, or if the second
Z-Calibration failed, perform the S&S calibration
(refer to 1 mL Subsystem S&S Calibrations for
Z-Calibration on page 5–43) and move on to
Step 2.
– For sample aspiration and deep well plate issues, if
the sp calibration did not require adjustment, or if
the second Z-Calibration failed, proceed directly to
to Step 3.
2. If the S&S calibration resulted in a change, rerun the
Z-Calibration check. If the Z check passes, proceed to
the master mix portion of this check.
3. If the S&S calibration results in no change, or if the
Z-Calibration check fails, move the calibration in the
required direction (+ 3 steps) and rerun the
Z-Calibration check.
4. Repeat Step 3 until the Z-Calibration passes.

Procedure
1. Perform the following steps to set up the worktable:
• Load three full racks of 1000 µL DiTis on DiTi carrier
1, positions 1 and 2, and shelf location 2
• Load input sample racks with 48 tubes containing
740 µL of tap water each
• Load 48 reaction vessels in a 1 mL Subsystem carrier,
and place the carrier in heater zone 1
• Load one reagent vessel with a minimum of 30 mL tap
water onto reagent vessel position 6 (mElution™
Buffer site) on reagent vessel carrier 2 (no bar code is
needed).
• Load a clean DiTi tray and deep well plate onto the
DiTi reuse rack on DiTi rack carrier 1
• Load a clean deep well plate onto the Output Deck

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Maintenance and Diagnostic Procedures Section 5

2. Select <Orders>, <Sample Extraction>, then <Diagnostic


- Z-Calibration Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next>. The <Sample Extraction: Assay Details>
screen displays.
5. In the <Actual Concentration> fields, type in the same
numbers as those listed under <Target Concentration>.
Select <Next> at the lower-right corner of the screen.
6. Close the door and select <Scan>. Proceed with the run as
with a typical Sample Extraction run.
7. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
8. When the <Sample Extraction: Assay Assignment>
screen displays, select <Next>. Then, ignore the <Sample
Extraction: Warnings> screen and select <Next> again.
9. At the <Sample Extraction: Worktable Setup> screen,
verify the 1 mL Subsystem carrier is in heater zone 1 and
that it contains 48 reaction vessels, then select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there are full 1000 µL DiTi racks at
locations 1 and 2 of DiTi rack carrier 1, and in shelf
location 2. Update the status, if necessary, and select
<Next>.
10. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
11. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
12. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
13. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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Section 5 System Checks

14. Open the safety door and visually inspect the reaction
vessels in the 1 mL Subsystem. There should be minimal
liquid residue in the reaction vessels.
The deep well plate on the Output Deck should show a
uniform distribution of fluid over the first 48 wells; each
well should contain about the same amount of liquid. If
these conditions have been met, proceed with the Master
Mix Addition portion of this test.

1 mL Subsystem S&S Calibrations for Z-Calibration


NOTE: These procedures are only to be used if the
Z-Calibration Test – Sample Extraction failed and you
have been instructed to perform them under Actions
above.
To access S&S functions, navigate to <System>, <Setup and
Service>. Then select <System Devices>,<LiHa>.

Calibrating Heater Zone 1 or 2


Mount a tip on Tip Adapter 3. Place an RV in position 20 of
the 1 mL Subsystem carrier and place the carrier in the heater
zone to be calibrated.
1. Using the LiHa Move tab, position the DiTi above the RV
and center it in the X and Y directions.
2. Use the keypad to lower the DiTi in the Z direction to the
bottom of the tube. Ensure the DiTi remains centered in
the X and Y directions.
3. Use your fingers to try and raise the tube. It should NOT
be able to move freely in the Z direction.
4. Using the Single Commands Tool, obtain the Z position of
the tip by entering the following command: A1RPZ0.
The answer will appear as A1@Z1,Z2,Z3,Z4,Z5,Z6,Z7,Z8.
Write down the number for Z3 plus 264 (the length of the
tip)
5. Open the m2000sp application and proceed to the
calibration screen for the location in question.
6. Input the Z-value obtained above (well bottom + 264).
7. Select <Move> before selecting <Finish> to complete the
calibration.

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Maintenance and Diagnostic Procedures Section 5

Calibrating Magnetic Rest


Mount a tip on Tip Adapter 3. Place an RV in position 20 of
the 1 mL Subsystem carrier and place the carrier in the
MagRest position.
1. Using the LiHa Move tab, position the DiTi above the RV
and center it in the X and Y directions.
2. Use the keypad to lower the DiTi in the Z direction to the
bottom of the tube. Ensure the DiTi remains centered in
the X and Y directions.
3. Use your fingers to try and raise the tube. It should NOT
be able to move freely in the Z direction.
4. Using the Single Commands Tool, obtain the Z position of
the tip by entering the following command: A1RPZ0.
The answer will appear as A1@Z1,Z2,Z3,Z4,Z5,Z6,Z7,Z8.
Write down the number for Z3 plus 334 (the length of the
tip and the carrier)
5. Open the m2000sp application and proceed to the
calibration screen for the MagRest position.
6. Input the Z-value obtained above (well bottom + 334).
7. Select <Move> before selecting <Finish> to complete the
calibration.

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Section 5 System Checks

Z-Calibration Check – Master Mix

Purpose

This Master Mix Addition step completes the Z-calibration test


begun with Sample Extraction. This test will take the plate
created in the previous test and complete the Master Mix
Addition process.

Actions
At the end of the procedure, be sure to:
• Verify that no errors occurred (if any occur, the test has
failed)
• Ensure the first 48 wells in the PCR plate contain an equal
amount of fluid: if the fluid level is inconsistent, check the
system fluidics for leaks

Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Beginning at position 1, load two reagent packs (no
bar code needed) and reagent vials filled with tap
water in the volumes specified below:
Table 5.5: Tap Water Volumes

Vial Position Target Fill Volume


1 0.40 mL
2 1.05 mL
3 0.14 mL

• Load a clean master mix tube at position 1 on the


master mix tube holder
• Ensure that the deep well plate created in the previous
Z-Calibration Check - Sample Extraction is located on
the Output Deck
• Load a clean PCR plate on the chiller

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Maintenance and Diagnostic Procedures Section 5

2. Select <Orders> and then <Master Mix Addition>. In the


<Select a Plate> field, highlight the plate that was run in
the first portion of this buffer run, and then select <Set Up
Run> from the <Master Mix Addition Tasks> menu.
3. Close the door and perform the protocol as a typical
Master Mix Addition run. Select <Next> on the <Master
Mix Addition: Plate Details> screen.
4. At the <Master Mix Addition: Worktable Setup> screen,
enter arbitrary numbers for the <PCR Plate Name> and
select <Next>. Ignore all on-screen instructions.
When the <Master Mix Addition: Disposable Tip
Status> screen appears, verify there is a full 1000 µL DiTi
rack in location 1, on DiTi rack carrier 1, and a full 200 µL
DiTi rack in location 4, on DiTi rack carrier 2. Update the
status, if necessary, and select <Next>.
5. On the <Master Mix Addition: Assay Specific Reagent
Scan> screen, select <Scan>, then <Next>.
6. Verify the master mix tube is in the correct location as
indicated on the <Master Mix Addition: Run Start>
screen and select <Start>.
7. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
8. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
9. When the door is unlocked, visually inspect the PCR plate.
The first 48 wells should be filled with about the same
amount of fluid.

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Section 5 System Checks

RNA Simulation Test – Sample Extraction

Purpose
This is the first part of an RNA Simulation procedure in which
a buffer run will be performed to evaluate m2000sp
performance through an entire RNA extraction and master
mix sequence.
NOTE: Two 995 reagent bar codes and reagent packs,
and reagent vessel RNA bar codes, will be needed for
the master mix portion of this test.
In the Sample Extraction portion, all aspects of a typical RNA
run will be performed, except for duplicate washes or
incubation time.

Actions
As you run this procedure, be sure to:
• Examine each step of the process for correct calibrations
and adjust if necessary
• Examine each step of the process for correct liquid
handling: if fluid removals are not accurate, examine the
system fluidics for leaks
• Verify that no errors occurred during the run (if errors
occur, the test has failed)
After the procedure:
• Ensure the first 48 wells in the deep well plate contain an
equal amount of fluid: if there is inconsistency, check the
system fluidics for leaks

Procedure
1. Perform the following steps to set up the worktable:
• Load 13 mm sample racks with 48 tubes, containing a
minimum of 1.5 mL of tap water, starting with tube
position 1
• Load the 1 mL Subsystem carrier with 48 unused
reaction vessels, beginning at position 1, and place
the carrier in heater zone 1
• Load full 1000 µL DiTi racks on DiTi carrier 1,
positions 1 and 2, and in shelf positions 2-8

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Maintenance and Diagnostic Procedures Section 5

• Load reagent vessels with tap water in the locations


and volumes listed in the table below (RNA bar codes
are required)
Table 5.6: Reagent Vessel Volumes

Location Location Label Minimum Fill Volume


1 m™Lysis 140 mL
3 mMicroparticles 26 mL
4 mWash 1 92 mL
5 mWash 2 92 mL
6 mElution 22 mL

• Load a clean DiTi tray and deep well plate onto the
DiTi reuse rack on DiTi rack carrier 1
• Load a clean deep well plate onto the Output Deck

2. Select <Orders>, <Sample Extraction>, then <m2000


RNA Simulation Test>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next>. The <Sample Extraction: Assay Details>
screen displays.
5. In the <Actual Concentration> fields, type in the same
numbers as those listed under <Target Concentration>.
Select <Next> at the lower-right corner of the screen.
6. Close the door and select <Scan>. Proceed with the run as
with a typical Sample Extraction run.
7. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
8. When the <Sample Extraction: Assay Assignment>
screen displays, select <Next>. Then, ignore the <Sample
Extraction: Warnings> screen and select <Next> again.
9. At the <Sample Extraction: Worktable Setup> screen,
verify the 1 mL Subsystem carrier is in heater zone 1 and
that it contains the right number of reaction vessels in the
positions indicated on the screen, then select <Next>.

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Section 5 System Checks

When the <Sample Extraction: Disposable Tip Status>


screen appears, verify that for every 8 samples used above,
there is one full rack of 1000 µL DiTis on DiTi carrier 1,
positions 1 and 2, and in shelf positions 2-8, as needed.
Update the status, if necessary, and select <Next>.
10. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.

11. On the screen that follows, select <Scan> then <Next>.


On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
12. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
13. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

RNA Simulation Test – Master Mix

Purpose
This is the second portion of the buffer run procedure that
begins with the RNA Simulation Test – Sample Extraction. This
test will take the plate created in the previous test and
complete the master mix process.
NOTE: Two 995 reagent bar codes and reagent packs,
and reagent vessel RNA bar codes, will be needed for
this portion of this test.

Actions
As you run this procedure, be sure to:
• Examine each step of the process for correct calibrations
and adjust if necessary
• Examine each step of the process for correct liquid
handling: if fluid removals are not accurate, examine the
system fluidics for leaks
• Verify that no errors occurred during the run (if errors
occur, the test has failed)
After the procedure:
• Ensure the first 48 wells in the PCR plate contain an equal
amount of fluid: if there is inconsistency, check the system
fluidics for leaks

Procedure
1. Perform the following steps to set up the worktable:
• Load one full rack of 1000 µL DiTis on DiTi carrier 1,
position 1
• Load one full rack of 200 µL DiTis on DiTi carrier 2,
position 4
• Beginning at position 1, load two reagent packs (with
reagent bar code 995) and reagent vials filled with tap
water in the volumes specified in the table below
Table 5.7: Tap Water Volumes

Vial Position Target Fill Volume


1 0.40 mL

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Section 5 System Checks

Vial Position Target Fill Volume


2 1.05 mL
3 0.14 mL

• Load a clean master mix tube at the position that


corresponds to the left-most reagent pack location on
the master mix tube holder
• Ensure that the deep well plate created in the previous
RNA Simulation Test – Sample Extraction is located on
the Output Deck
• Load a clean PCR plate on the chiller
2. Select <Orders>, <Master Mix Addition>, then in the
<Select a Plate> field, select the plate that was run in the
first portion of the buffer run.
3. Select <Set Up Run> from the left-hand option menu.
4. Close the door and perform the protocol as a typical
Master Mix Addition run. Select <Next> on the <Master
Mix Addition: Plate Details> screen.
5. At the <Master Mix Addition: Worktable Setup> screen,
enter arbitrary numbers for the <PCR Plate Name> and
select <Next>. Ignore all on-screen instructions.
When the <Master Mix Addition: Disposable Tip
Status> screen appears, verify there is a full 1000 µL DiTi
rack in location 1, on DiTi rack carrier 1, and a full 200 µL
DiTi rack in location 4, on DiTi rack carrier 2. Update the
status, if necessary, and select <Next>.
6. On the <Master Mix Addition: Assay Specific Reagent
Scan> screen, select <Scan>, then <Next>.
7. Verify the master mix tube is in the correct location as
indicated on the <Master Mix Addition: Run Start>
screen and select <Start>.
8. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
9. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.
10. When the door is unlocked, visually inspect the PCR plate.
The wells used in the test should be filled with about the
same amount of fluid.

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Maintenance and Diagnostic Procedures Section 5

Other Checks
Chiller and Heater Temperature Check

Purpose
During this test, the set temperatures at heater zone 1, heater
zone 2, and the PCR tray holder will be confirmed.

Actions
If either heater zone fails to come to temperature:
• Check the cabling at the temperature block; check the
tightness of the connections
• Check the heater block hardware (heater boards, etc.)
If the chiller fails to reach temperature:
• Check the cabling at the chiller
• Check the chiller hardware

Procedure
1. Perform the following steps to set up the worktable:
• Load a reaction vessel with 1 mL of water in column
3, position 4 of the 1 mL Subsystem carrier and install
the carrier cover
• Place the 1 mL carrier in heater zone 1
2. Select <System> then <Maintenance Procedures> or
select the Maintenance Procedure icon.
3. Select <Chiller and Heater Temperature Check> from
the <Maintenance Procedures> screen and then select
<Set Up Run> from the <Maintenance Tasks> menu on
the left.
4. Close the door and select <Start> to begin the test. The
chiller temperature will begin to descend, and the system
will automatically pause, allowing the operator to open
the door.
5. Set the TVK into the PCR plate holder on the chiller. The
TVK should remain there throughout the test to allow
temperature readings to be taken periodically over the
next 20 minutes.

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Section 5 Other Checks

6. When the temperature is within range (8°C, ± 3°C) or


when 20 minutes of the test are over, close the door and
press <Resume>.
The temperature in heater zone 1 will begin to ramp, and
the system will automatically pause, allowing the
operator to open the door.
7. Set a calibrated thermometer into the reagent vessel in the
1 mL Subsystem carrier. The thermometer should remain
there throughout the test to allow temperature readings to
be taken periodically over the next 40 minutes.
8. When the temperature is within range (50°C, ± 3°C) or
when 40 minutes of the test are over, close the door and
press <Resume>.
9. The temperature in heater zone 2 will begin to ramp, and
the system will automatically pause, allowing the operator
to open the door.
10. Move the Subsystem carrier along with the calibrated
thermometer to heater zone 2.
11. When the temperature is within range (75°C, ± 3°C) or
when 60 minutes of the test are over, close the door and
press <Resume>.
12. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
13. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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Maintenance and Diagnostic Procedures Section 5

Ethanol LLS Check

Purpose
During this buffer run procedure, liquid level sensing is
performed six times for mWash 2DNA.

Actions
If level sensing fails:
• Check that the correct amount of fluid was added to the
trough
• Check the connections for the level sensing circuit (ILID
board, ILID cable, tip adapter)
• Check the hardware for the level sensing circuit (ILID
board, ILID cable, tip adapter)
• Retry the test

Procedure
1. Perform the following steps to set up the worktable:
• Load a full rack of 1000 µL DiTis on position 1 of DiTi
rack carrier 1
• Load a 13 mm input sample rack with 8 tubes
• Load one reagent vessel with a minimum 30 mL of
70% Ethanol solution into reagent vessel position 5
(no bar code is needed)
2. Select <Orders>, <Sample Extraction>, then <Ethanol
LLS Check>.
3. Select <Set Up Run> from the left-hand option menu.
4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen to
move on.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
At the <Sample Extraction: Worktable Setup> screen,
ignore all instructions and select <Next>.

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Section 5 Other Checks

When the <Sample Extraction: Disposable Tip Status>


screen appears, verify there is a full 1000 µL DiTi rack at
location 1 of DiTi rack carrier 1. Update the status, if
necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.
On the <Sample Extraction: Run Start> screen, ignore
all on-screen instructions and select <Start>.
The LiHa stakes tips, moves to the reagent vessel, and
performs a 10 µL aspiration six times.
9. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
10. Select <Close Process> from the side panel to close the
test, and confirm with <Yes>.

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Maintenance and Diagnostic Procedures Section 5

LiHa Sample Volume Checks

Purpose
These checks determine the minimum sample volume needed
for a given tube and a given sample aspiration volume in order
to achieve reliable aspiration.
There are four sample volumes that can be checked:
• 200 µL
• 400 µL
• 500 µL
• 600 µL
• 1000 µL
These checks are particularly useful for determining the
minimum sample volume required in custom tubes. Refer to
Sample Rack and Tube Configuration on page 4–149 of Section
4, Installation, for more information on custom tubes.

Actions
The test should be considered a failure after two unsuccessful
100 µL water additions.
If failure occurs:
• Ensure that tap water, and NOT deionized or distilled
water, was used
• Check the connections for the level sensing circuit (ILID
board, ILID cable, tip adapter)
• Check the hardware for the level sensing circuit (ILID
board, ILID cable, tip adapter)

Procedure
1. Perform the following steps to set up the worktable:
• Prepare 13 or 16 mm sample racks with a minimum of
8 tubes, starting with tube position 1. Fill each tube
with tap water in the sample volume desired (200,
400, 500, 600, or 1000 µl) plus an additional 200 µL
• Load the sample racks on the worktable

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Section 5 Other Checks

• Load the 1 mL Subsystem carrier with the same


number of unused reaction vessels, beginning at
position 1, and place the carrier in heater zone 1
• Load full racks of 1000 µL DiTis on DiTi carrier 1,
positions 1 and 2

2. Select <Orders> and <Sample Extraction>, then choose


the check that matches the sample volume desired:
• <Diagnostic – LiHa Sample Volume 200 Check>
• <Diagnostic – LiHa Sample Volume 400 Check>
• <Diagnostic – LiHa Sample Volume 500 Check>
• <Diagnostic – LiHa Sample Volume 600 Check>
• <Diagnostic – LiHa Sample Volume 1000 Check>

3. Select <Set Up Run> from the left-hand option menu.


4. The <Sample Extraction: Run Setup> screen displays.
Select <Next> at the lower-right corner of the screen.
5. Select <Scan> to scan the samples. Proceed with the run as
with a typical Sample Extraction run.
6. Enter arbitrary numbers for the <Sample ID> when
prompted. Select <Next>.
At the <Sample Extraction: Worktable Setup> screen,
ignore the on-screen instructions. Verify the 1 mL
Subsystem carrier is in heater zone 1, then select <Next>.
When the <Sample Extraction: Disposable Tip Status>
screen appears, verify there are full 1000 µL DiTi racks at
locations 1 and 2 of DiTi rack carrier 1. Update the status,
if necessary, and select <Next>.
7. At the <Sample Extraction: Reagent Setup> screen, enter
arbitrary numbers for <Deep Well Plate ID> and
<Reagent Lot Number>, and the current month and year
for <Expiration Date>. When ready, select <Next>.
NOTE: Disregard all on-screen information and
instructions; these are extraction run instructions
and are irrelevant to this diagnostic test.
8. On the screen that follows, select <Scan> then <Next>.

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200942-103—September 2007
Maintenance and Diagnostic Procedures Section 5

9. On the <Sample Extraction: Run Start> screen, ignore all


on-screen instructions and select <Start>.

If the Test Passes


If the check completes successfully, a minimum volume has
been determined. However, operators who ran a
higher-volume check initially could run the check again at a
lower volume to see if that will work as well.
When the process is complete, select <Close>, then <Close>
again to clear the message boxes.
Select <Close Process> from the side panel to close the test,
and confirm with <Yes>.

If the Test Fails


If a test fails, it will need to be rerun with a higher volume.
Select <Close>, then <Close> again to clear the message boxes.
Select <Close Process> from the side panel to close the test,
and confirm with <Yes>. Then, proceed as follows:

a. Return to Step 1 and reload the tubes. Use the same


sample volume desired, but this time increase the
additional volume by 100 µL.
NOTE: It will probably not be necessary to
reload DiTi racks at each check attempt. Ensure
you have at least as many DiTis as sample tubes
before each attempt.
Repeat this process until the check proceeds
successfully.
b. When the process is completed, select <Close>, then
<Close> again to clear the message boxes.
c. Select <Close Process> from the side panel to close
the test, and confirm with <Yes>.

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2000942-103—September 2007
Module 6: Upgrade Procedures

SECTION 6:
UPGRADE PROCEDURES

m2000sp G-series Service Manual 6-1


200942-103—September 2007
Upgrade Procedures Section 6

NOTES

6-2 m2000sp G-series Service Manual


200942-103—September 2007
Section 6 Table of Contents

Table of Contents

Procedural Resources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4


m1000 to m2000sp Conversion . . . . . . . . . . . . . . . . . . . 6-4
Hood Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
Ramped Waste Chute Installation . . . . . . . . . . . . . . . . . . . . 6-5

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200942-103—September 2007
Upgrade Procedures Section 6

Procedural Resources

m1000 to m2000sp Conversion


Directions for upgrading an m1000 instrument to an m2000sp
instrument are contained in the latest version of TSB 605-002.

Hood Installation
Hoods are not standard equipment on all G-series instruments.
When installation is required, refer to the latest revision of TSB
605-018.

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200942-103—September 2007
Section 6 Ramped Waste Chute Installation

Ramped Waste Chute Installation


Ensure the waste station chute is securely positioned and that
the ramped section at the bottom of the chute points to the
left, toward the center of the solid waste container.

A warning sticker
shows when the
waste chute is
being installed
incorrectly.

A green circle
appears on the
front of the waste
chute.

Incorrect Figure 6.1: Waste Chute Properly Positioned


positioning of
the waste
IMPORTANT: If the waste chute’s red warning
chute could sticker is visible after it is reinstalled, it has been
result in errors positioned incorrectly. The green circle label
must face the user.

After the chute is installed correctly, it will be necessary to


reset the sensor height by recalibrating the DiTi waste as
described below.

Calibrate the DiTi Waste Sensor


Adjustments and calibrations are performed through the
Genesis S&S software.
NOTE: To access S&S, log on to the SCC and select
<System>, <Setup and Service>.

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200942-103—September 2007
Upgrade Procedures Section 6

1. Go to the <Options>, <SMIO for NAT> menu.

Figure 6.2: Options Menu

2. Select <DiTi Waste Sensor Calibration> under <Setup>.

Figure 6.3: SMIO Panel Menu

3. Go to the <Tools & Configuration> tab.

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200942-103—September 2007
Section 6 Ramped Waste Chute Installation

4. Verify the <Device Type> is <NAT>. (If not, select <NAT>


from the pull-down menu.) Then select <Diti Waste Full>.
Clear the other checkboxes.

Figure 6.4: Tools & Configuration Tab

5. Select <Start>. The prompt <Place a reflecting plate at


about 120 [mm] below the ultrasonic sensor> displays.
Instead of placing the plate at 120 mm, place the plate at
300 mm below the sensor.

Figure 6.5: Process Prompt: Reflector Plate

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200942-103—September 2007
Upgrade Procedures Section 6

6. Select <Next>. The prompt <Turn both potentiometers


counterclockwise to their stop positions> displays.
NOTE: The potentiometers are made of plastic. Be
careful not to damage them by applying too much
force.

Figure 6.6: Process Prompt: Potentiometers

7. Select <Next>. The prompt <Adjust the distance


potentiometer (top potentiometer) to the point where
the integrated LED just lights up> displays.

Figure 6.7: Process Prompt: Adjust Potentiometers

8. Select <Next>. The prompt <Setup: Done> displays.

Figure 6.8: Process Prompt: Setup

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200942-103—September 2007
Section 6 Ramped Waste Chute Installation

9. Select OK.
NOTE: If the calibration fails, check the
connection to the SMIO board and repeat the test.
NOTE: If the calibration fails again, replace the
sensor.
10. Perform the DiTi Waste Sensor Verification.

DiTi Waste Sensor Verification


1. From S&S, go to <Options>, <SMIO for NAT>.
2. Select the <Sensors> test box.
3. Go to the <Tools & Configuration> tab and check the
<DiTi Waste Full> box, ensuring that no other boxes are
checked.
4. Select <Start>. The prompt <Place a reflecting plate at
180 [mm] below the sensor> displays. Instead of placing
the plate at 180 mm, place the plate at 360 mm below the
sensor.

Figure 6.9: Process Prompt: Reflecting Plate

5. Select <Next>. The prompt <Place the plate at 120 [mm]


below the sensor> displays. Instead of placing the plate at
120 mm, place the plate at 300 mm below the sensor.

Figure 6.10: Process Prompt: 120 mm

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200942-103—September 2007
Upgrade Procedures Section 6

6. Select <Next>. If the test was successful, the prompt


<Sensor Test: Passed> displays.

Figure 6.11: Test Passed

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200942-103—September 2007
Section 7: Troubleshooting

SECTION 7:
TROUBLESHOOTING

m2000sp G-series Service Manual 7-1


200942-103—September 2007
Troubleshooting Section 7

NOTES

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200942-103—September 2007
Section 7 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-5
Error Codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
Process Management Issues (Error Codes 1000-1099) . . 7-6
System Calibration Issues (Error Codes 1100-1199) . . . . 7-6
Test Order Issues (Error Codes 1200-1299) . . . . . . . . . . . 7-6
Reagent Management (Error Codes 1300-1399) . . . . . . . 7-7
Check Configuration (Error Codes 1500-1599) . . . . . . . 7-7
Signal Tower (Error Codes 1600-1699) . . . . . . . . . . . . . . 7-8
LiHa Calibration (Error Codes 2000-2099) . . . . . . . . . . . 7-8
RoMa Calibration (Error Codes 2100-2199) . . . . . . . . . . 7-8
Run Application (Error Codes 2200-2299) . . . . . . . . . . . 7-8
Run Master Mix Addition (Error Codes 2300-2379) . . . 7-8
Hardware Configuration (Error Codes 2380-2399) . . . . 7-9
Shared Sample Preparation (Error Codes 2400-2499) . . 7-9
Instrument Control (Error Codes 2500-2599) . . . . . . . . 7-9
Sample Preparation System Calibrations (Error Codes
2601-2699) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-10
Sample Preparation Command Library (Error Codes
2700-2799) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-11
Sample Preparation Data Server (Error Codes 2800-2899)
Robotic Sample Processor (RSP) COM (Error Codes
3040-3099) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-13
Liquid Handler (LiHa) (Error Codes 3101-3199) . . . . . 7-13
Dilutor (Error Codes 3201-3299) . . . . . . . . . . . . . . . . . 7-22
Robotic Manipulator (RoMa) (Error Codes 3301-3399) 7-23
Bar Code Scanner (PosID) (Error Codes 3401-3499) . . 7-25
Doorlocks (Error Codes 3502-3599) . . . . . . . . . . . . . . . 7-26
Fast Wash Pump (FaWa) (Error Codes 3602-3699) . . . . 7-27
Heaters (Error Codes 3701-3799). . . . . . . . . . . . . . . . . . 7-27
SMIO (Smart Monitored Input/Output) (Error Codes
3802-3899) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-27
Output Deck Chiller (Error Codes 3900-3949) . . . . . . . 7-28
Hood (Error Codes 3950-3999) . . . . . . . . . . . . . . . . . . . 7-29
Disposable Tip Inventory (Error Codes 7000-7099) . . . 7-29
User Notification (Error Codes 7100-7199) . . . . . . . . . 7-29
Application Specification (Error Codes 7200-7299) . . . 7-29
Result Management (Error Codes 7300-7399) . . . . . . . 7-30
Backup and Restore (Error Codes 7400-7499 / 8700-8799) .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-30
Shared Application Specification (Error Codes 7600-7749) .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-31
Network Data Store (Error Codes 7750-7799) . . . . . . . 7-31

m2000sp G-series Service Manual 7-3


200942-103—September 2007
Troubleshooting Section 7

Report Requests (Error Codes 7900-7999) . . . . . . . . . . 7-31


User Access (Error Codes 8000-8049 / 8400-8499) . . . . 7-32
Network Configuration (Error Codes 8050-8099) . . . . 7-32
Audible Alert (Error Codes 8100-8299) . . . . . . . . . . . . 7-32
Date/Time Configuration (Error Codes 8500-8599) . . 7-33
Serial Number (Error Codes 8600-8699) . . . . . . . . . . . . 7-33
Operating System (Error Codes 8800-8899) . . . . . . . . . 7-33
Language and Locale (Error Codes 8900-8910). . . . . . . 7-33
On-line Documentation (Error Codes 8911-8950) . . . 7-33
Architecture (Error Codes 9000-9099). . . . . . . . . . . . . . 7-33
Hardware Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-34
CAN BUS Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-34
Dilutor Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-34
Doorlocks Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-36
Edgeport™ Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . 7-37
FaWa Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-37
Heater Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-38
LiHa Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-39
Output Deck Hardware . . . . . . . . . . . . . . . . . . . . . . . . . 7-44
PosID Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-44
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-47
Printer Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-48
RoMa Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-48
SCC Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-54
Sensor Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-57
SMIO/SMIO-SAFY Hardware . . . . . . . . . . . . . . . . . . . . . 7-58
Instrument Connection Issues . . . . . . . . . . . . . . . . . . . 7-58
Database/Restore Issues . . . . . . . . . . . . . . . . . . . . . . . . 7-59
Network Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-59
Signal Tower Hardware . . . . . . . . . . . . . . . . . . . . . . . . . 7-59

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200942-103—September 2007
Section 7 Purpose of This Section

Purpose of This Section


This section provides information necessary to resume
operation of the m2000sp after a physical problem has
occurred that cannot be eliminated by the operator. Before
performing any troubleshooting refer to Section 2, Safety for
instrument and non-instrument hazards and safety measures.

NOTE: For software troubleshooting and diagnostics,


refer to Section 10, Troubleshooting, of the m2000sp
Operations Manual.

Observe the following safety notices.


WARNING: Potential Biohazard. Indicates an area
where the FSE or operator may be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator may
be exposed to hazardous chemicals when handling
Abbott Molecular sample preparation reagents. The
exposure to hazardous chemicals is minimized by
following instructions provided in product-specific
labels and product-specific Material Safety Data Sheets
(MSDS). Wear appropriate personal protective
equipment, such as gloves, lab coat, and protective
eyewear.
WARNING: Class II Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing of the bar code laser beam or
reflections of the beam.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

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200942-103—September 2007
Troubleshooting Section 7

Error Codes

The following section contains general information on all


error codes for the m2000sp system, as well as a series of
possible troubleshooting steps to resolve each error. For a
complete list of error codes, refer to Section 10,
Troubleshooting, of the m2000sp Operations Manual.

Process Management Issues (Error Codes 1000-1099)

Timing exceeded / Application aborted


• Check for related errors (DiTi staking, liquid level sense) in
the Process Log and correct the area of error
• Reinitialize the instrument
• Repeat the process
The majority of these error codes relate to action during
processing (completion of run, pause requested, etc.). Action is
not required for these error codes.

System Calibration Issues (Error Codes 1100-1199)


These errors occur during calibration. Wait for a valid
instrument state to perform calibration functions.

Test Order Issues (Error Codes 1200-1299)

Bar Code Missing / Incorrect / Unreadable


• Load required carriers, reagent packs, samples, etc., on the
worktable
• Load the carrier in the correct location (as indicated on
screen)
• Check that all racks and carriers have the correct prefix on
the bar codes
• Check that all sample input racks have unique bar code
IDs

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200942-103—September 2007
Section 7 Error Codes

• Check that all racks and carriers are in the same position
as during the initial sample scan
• Verify carriers and racks are enabled
• Check that all bar codes meet specifications and are
configured correctly

Sample Rack Not Enabled / Empty / Missing Sample


• Use a different sample rack
• Enable and calibrate the current rack
• Check tube sensor on the PosID

Sensor Check Failed or Tripped


• Check that the liquid waste is emptied, system liquid is
filled, and solid waste is emptied
• If you suspect a hardware issue, refer to Sensor Hardware
on page 7–57

Reagent Management (Error Codes 1300-1399)


Bar Code Invalid / Wrong Size/ Unreadable
• Replace the reagent pack
• Check the quality of the bar code
• Clean the PosID head
• Adjust the PosID through S&S

Too Many / Too Few Assay Reagent Packs Present


• Load the correct number of reagent packs
• Load Genotype packs in order

Check Configuration (Error Codes 1500-1599)


• Cycle power on the SCC and the system

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200942-103—September 2007
Troubleshooting Section 7

• Perform a system backup


• If necessary, reload software

Signal Tower (Error Codes 1600-1699)


• Check cable connections at Edgeport and SCC
• Check cable connections at hood controller board
• Cycle power on the SCC and the instrument
• Ι f you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37
• If you suspect a hardware issue, refer to Signal Tower
Hardware on page 7–59

LiHa Calibration (Error Codes 2000-2099)


The majority of these errors codes are user notification
prompts. Follow the instructions given on the prompts.

RoMa Calibration (Error Codes 2100-2199)


The majority of these errors codes are user notification
prompts. Follow the instructions given on the prompts.

Run Application (Error Codes 2200-2299)


These error codes are generally the result of user error
(incorrect date entry, concentration value, etc.). Follow the
instructions given on the prompts.

Run Master Mix Addition (Error Codes 2300-2379)


These error codes are generally the result of user error
(incorrect lot number entry, etc.). Follow the instructions
given on the prompts.

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200942-103—September 2007
Section 7 Error Codes

Hardware Configuration (Error Codes 2380-2399)


These errors relate to actions during hardware configuration,
and occur while in the Hardware Configuration screen. Follow
the screen prompts to resolve these errors. Refer to Hardware
Configuration on page 4–44.

Shared Sample Preparation (Error Codes 2400-2499)


The majority of these errors codes are user notification
prompts (need for a commodity, etc.). Follow the instructions
given on the prompts.

Instrument Control (Error Codes 2500-2599)

General Errors
• Cycle power on the SCC and the instrument
The majority of these errors codes are user notification
prompts. Follow the instructions given on the prompts.

Sensor Errors
• Check full/empty status of the indicated sensor
• Cycle power on the SCC and the instrument

Chiller Error Code


• Check the seating of the power cable at the chiller
• Check the Edgeport™ cable connections
• Cycle power on the chiller
• Unplug and replug the Edgeport
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37

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200942-103—September 2007
Troubleshooting Section 7

Doorlock Error Codes


• Check that the door is completely closed and nothing is
obstructing the path of the doorlocks
• Check for damage to the doorlock fingers
• Cycle power on the SCC and the instrument
• Retry the process
• If you suspect a hardware issue, refer to Doorlocks
Hardware on page 7–36

Sample Preparation System Calibrations (Error Codes 2601-2699)

Calibration File Corrupted / Missing / Failed to Save


• Cycle power on the SCC and the instrument
• Repeat system calibration procedures
• Save calibrations after each system calibration

Failed to Restore Calibration File


• Cycle power on the SCC and the instrument
• Wait until CD spins up before selecting restore
• Retry restore function
• Repeat system calibration procedures

LiHa/RoMa Unavailable / Failure Reported During Travel


• Check for obstructions of the LiHa or RoMa
• Cycle power on the SCC and the instrument
• Retry movement

RoMa Failed to Grip Object


• Check that the object is present and seated correctly
• Cycle power on the SCC and the instrument
• Check for obstructions to the RoMa movement

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200942-103—September 2007
Section 7 Error Codes

• Check the RoMa calibrations

Unable to Stake a Tip


• Check that full DiTi racks are in place
• Update the disposable tip inventory status (navigate to
<System>,<Disposable Tip Status>)
NOTE: The DiTi inventory present on the
worktable and shelves must match the system’s
understanding of the inventory as depicted on
screen. Refer to Calibrate Disposable Tip Locations
on page 4–153 of Section 4.
• Recalibrate the DiTi locations

Sample Preparation Command Library (Error Codes 2700-2799)

DiTi Errors
• Check that all DiTi cans are loaded in the correct location
• Update the disposable tip inventory status (navigate to
<System>,<Disposable Tip Status>)
NOTE: Refer to Calibrate Disposable Tip Locations
on page 4–153.

Heater/Chiller Errors
• Cycle power on the SCC and the instrument
• Cycle power on the chiller
• Unplug and replug the Edgeport™
• Check cable connections
• CheckHeater and CheckChiller turned off errors are due to
Application Specification problems; contact your local FAS
• If runs are performed back-to-back, the heater might not
be able to cool down to the start temperature fast enough
• If you suspect a hardware issue, refer to Heater Hardware
on page 7–38

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200942-103—September 2007
Troubleshooting Section 7

• If you suspect a hardware issue, refer to Output Deck


Hardware on page 7–44

Process/Command Stopped Because of Failed Samples


• Check the logs and troubleshoot devices as appropriate
• Rerun samples if possible

Process/Command Aborted
• Check the logs and troubleshoot devices as appropriate
• Cycle power to the instrument
• Check that communication with the instrument has been
established
• If error occurred in the master mix
– check for sufficient fluid in the reagent pack and
master mix tube
– check for bubbles in the reagent pack
• If error occurred during extraction, check that the 1 mL
Subsystem carrier or carriers have been placed correctly
• Check that all commodities have been placed correctly
• Rerun the samples if possible

Command Skipped / 1 mL Subsystem Carrier Absent


These are status messages. No action is necessary. The
commands are skipped because the plate was not used or all
samples in that plate have failed.

Sample Preparation Data Server (Error Codes 2800-2899)


• Retry the process
• Cycle power on the SCC and the instrument
• Perform a system backup
• If necessary, re-ghost the system

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200942-103—September 2007
Section 7 Error Codes

Robotic Sample Processor (RSP) COM (Error Codes 3040-3099)


• Verify the cable connections at the instrument and the
SCC
• Check the Genesis logs for specific device failures
• Troubleshoot the particular device failure encountered

Liquid Handler (LiHa) (Error Codes 3101-3199)

Initialization Error
• Check for obstructions to the LiHa
• Cycle power to the instrument and reinitialize

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process
• If error occurred during extraction, ensure that adequate
sample volume (per package insert) is present

Drive No Load Errors


• Check for obstructions on the LiHa
• Check that all carriers, commodities, etc., are in the
correct location
• Check that the right rack/tube configuration was used
• Check that adequate sample volume is present in the tubes
• Verify that the DiTis are not building up in the waste
container
NOTE: It is good practice to empty the waste
container prior to a run of more than 48 samples.
• If you suspect a hardware issue, refer to LiHa Hardware on
page 7–39

Tip Errors
• Verify that tips are available

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200942-103—September 2007
Troubleshooting Section 7

• Check the LiHa calibration


• Update the disposable tip inventory status (navigate to
<System>,<Disposable Tip Status>)
NOTE: Refer to Calibrate Disposable Tip Locations
on page 4–153 of Section 4.
• If you suspect a hardware issue, refer to LiHa Hardware on
page 7–39

Not Enough Liquid Errors


• Verify that adequate liquid is available
• Check ILID system connections
• Check liquid for bubbles
• Check LiHa calibration
• Check lines/syringes for bubbles
• Perform an Extensive Flush on page 5–10 of Section 5

3109 (No Liquid Detected)


• Sample Troubleshooting
– Verify that adequate liquid is available
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check tip adapters
– Check the ILID cable connections
– Check the ILID cables
– Check the ILID board
• Instrument Troubleshooting
– If there are consistent No Liquid Detected errors,
check the Z-calibration at the problem location
• S&S troubleshooting
– Perform Liquid Detection on page 4–127 of Section 4
to verify correct function of the ILID circuitry

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200942-103—September 2007
Section 7 Error Codes

• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance
NOTE: There should be no attempt to retry at
the trough or heater zone 1.
• Log Troubleshooting
– Check the run logs (Process Log, Message History Log,
Process.LiHa.log) to see if a specific tip adapter has
been having consistent level sense errors, which
might indicate a hardware failure of that tip
adapter/ILID cable/ILID board

3119 (No Clot Detected)

Figure 7.1: No Clot Detected

• Sample Troubleshooting
– Check liquid for clots
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
– Check that the sample was vortexed/centrifuged
correctly
• Hardware Troubleshooting
– Check tip adapters

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200942-103—September 2007
Troubleshooting Section 7

– Check the ILID cable connections


– Check the ILID cables
– Check the ILID board
– Check the integrity of the fluidics system
NOTE: If the problem is specifically noted on tips
1-4 or 5-8, check the 1:4 manifolds on the top of
the system.
• Instrument Troubleshooting
– Check the calibrations at the problem location
• S&S troubleshooting
– Perform a liquid detection test, increasing the clot
detection distance to 40
NOTE: If the test fails, check the Z-rods for
excessive hysteresis.
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance: after each fluid transfer,
pause to check for correct volumes; check for correct
DiTi centering throughout the test
• Log Troubleshooting
– Match failures in the logs (Process Log,
process.liha.log) with hardware failures
– Note the failure positions and volume trends (additive
failures) from previous steps

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200942-103—September 2007
Section 7 Error Codes

3130 (Less Liquid Than Expected)

Figure 7.2: Less Liquid Than Expected

• Sample Troubleshooting
– Check for proper volumes in reagent vessels and input
samples
– Check liquid for clots
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check all liquid system connections
– Inspect system liquid tubing
– Check the syringes, valves, and dilutors
– Check for large bubbles forming on the top of the
syringe
– Check the integrity of the fluidics system
NOTE: If the problem is specifically noted on tips
1-4 or 5-8, check the 1:4 manifolds on the top of
the system.
• Instrument Troubleshooting
– Check the calibrations at the problem locations

m2000sp G-series Service Manual 7-17


200942-103—September 2007
Troubleshooting Section 7

– Check the instrument Y-spread


• S&S troubleshooting
– Perform a liquid detection test, increasing the clot
detection distance to 40
NOTE: If the test fails, check the Z-rods for
excessive hysteresis.
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance: after each fluid transfer,
pause to check for correct volumes; check for correct
DiTi centering throughout the test
• Log Troubleshooting
– Match failures in the logs (Process Log,
process.liha.log) with hardware failures
– Note the failure positions and volume trends (additive
failures) from previous steps

3131 (More Liquid Than Expected)

Figure 7.3: More Liquid Than Expected

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200942-103—September 2007
Section 7 Error Codes

• Sample Troubleshooting
– Check liquid for clots
– Check liquid for bubbles
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check all liquid system connections
– Inspect system liquid tubing
– Check the syringes, valves, and dilutors
– Check for large bubbles forming on the top of the
syringe
– Check the integrity of the fluidics system
NOTE: If the problem is specifically noted on tips
1-4 or 5-8, check the 1:4 manifolds on the top of
the system.
• Instrument Troubleshooting
– Check the calibrations at the problem locations
– Check the instrument Y-spread
• S&S troubleshooting
– Perform a liquid detection test, increasing the clot
detection distance to 40
NOTE: If the test fails, check the Z-rods for
excessive hysteresis.
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance: after each fluid transfer,
pause to check for correct volumes; check for correct
DiTi centering throughout the test
• Log Troubleshooting
– Match failures in the logs (Process Log,
process.liha.log) with hardware failures
– Note the failure positions and volume trends (additive
failures) from previous steps

m2000sp G-series Service Manual 7-19


200942-103—September 2007
Troubleshooting Section 7

3152 (Specified Submerge Position for the Requested


Liquid Volume Exceeds the Calibrated Z-Bottom)

Figure 7.4: Liquid Volume Exceeds Z-Bottom

• Sample Troubleshooting
– Check for sufficient volume in sample tubes
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check tip adapters
– Check ILID cable connections
– Check ILID cables
– Check ILID board
– Examine the 1 mL Subsystem carrier plate for
deformity
• Instrument Troubleshooting
– Check the calibrations at the problem locations
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5

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200942-103—September 2007
Section 7 Error Codes

– Perform the Z-Calibration Check – Sample Extraction


on page 5–40 and Z-Calibration Check – Master Mix
on page 5–45 of Section 5
• Log Troubleshooting
– Check the logs for consistency of calibration values
and fluid removals

3153 (Insufficient Volume for Aspirate)

Figure 7.5: Insufficient Volume

• Sample Troubleshooting
– Check for sufficient volume in sample tubes
– Check that the right rack/tube configuration was used
• Hardware Troubleshooting
– Check tip adapters
– Check ILID cable connections
– Check ILID cables
– Check ILID board

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200942-103—September 2007
Troubleshooting Section 7

– Check the integrity of the fluidics system


NOTE: If the problem is specifically noted on tips
1-4 or 5-8, check the 1:4 manifolds on the top of
the system.
• Instrument Troubleshooting
– Check the calibrations at the problem locations
• S&S troubleshooting
– Perform a liquid detection test
• M&D Troubleshooting
– Perform an Extensive Flush on page 5–10 of Section 5
– Perform a Sample Extraction M&D to investigate
instrument performance: after each fluid transfer,
pause to check for correct volumes; check for correct
DiTi centering throughout the test
– Perform a Gravimetric Test
• Log Troubleshooting
– Match failures in the logs (Process Log,
process.liha.log) with hardware failures
– Note the failure positions and volume trends (additive
failures) from previous steps

Dilutor (Error Codes 3201-3299)

Dilutors Not Initialized


• Check the connection of the dilutor to the board
• Cycle power to the instrument
• Use the S&S Dilutor tool to independently verify the
initialization of the dilutors
• If you suspect a hardware issue, refer to Dilutor Hardware
on page 7–34

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

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Section 7 Error Codes

CAN BUS Error


• Cycle power on the SCC and the instrument
• If you suspect a hardware issue, refer to CAN BUS
Hardware on page 7–34

Plunger/Valve Overload
• Cycle power to the instrument
• For a plunger overload, replace the failed syringe, ensuring
it is properly seated in the thumb screw (refer to the logs if
the failed plunger cannot easily be seen)
• For a valve overload, replace the failed valve
• Replace the failed dilutor if necessary
• If you suspect a hardware issue, refer to Dilutor Hardware
on page 7–34

Piezo Module Error


• Cycle power to the instrument
• Check dilutor connections
• If you suspect a hardware issue, refer to Dilutor Hardware
on page 7–34

Robotic Manipulator (RoMa) (Error Codes 3301-3399)

Initialization Error
• Check for obstructions to the RoMa
• Cycle power to the instrument and reinitialize

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

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200942-103—September 2007
Troubleshooting Section 7

Plate Not Fetched Error


• Check that the object is in place
• Verify RoMa calibrations
• Check for any RoMa obstructions
• If you suspect a hardware issue, refer to RoMa Hardware on
page 7–48

Drive No Load Error


• Check for obstruction of the RoMa
• Check for correct placement of objects on the worktable
• Check for misalignment of the RoMa
• Check for misalignment of DiTi racks, DiTi rack carriers, or
the 1 mL Subsystem carrier
• Verify RoMa calibrations
• If you suspect a hardware issue, refer to RoMa Hardware on
page 7–48

Failed to Grip/Move Object


• Check for obstruction of the RoMa
• Check for correct placement of objects on the worktable
• Check for misalignment of the RoMa
• Check for misalignment of DiTi racks, DiTi rack carriers, or
the 1 mL Subsystem carrier
• Verify RoMa calibrations
• If you suspect a hardware issue, refer to RoMa Hardware on
page 7–48

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200942-103—September 2007
Section 7 Error Codes

Bar Code Scanner (PosID) (Error Codes 3401-3499)

Initialization Error
• Check for obstructions to the PosID
• Remove extra carriers at the end of the worktable
• Cycle power to the instrument and reinitialize
• Check frogs for wear

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

Drive No Load Error


• Check for obstruction of the PosID
• Check for correct placement of objects on the worktable
• Verify PosID mechanical adjustment
• If you suspect a hardware issue, refer to PosID Hardware on
page 7–44

Undefined Carrier Error


• Check that all carriers have the correct bar code identifiers
• Check that all bar codes are readable
• Check that all carriers are in the correct locations
• Check that the mounted rack is enabled
• If you suspect a hardware issue, refer to PosID Hardware on
page 7–44

Invalid Sample / Carrier Bar Code Error


• Check that the bar code is readable
• Check that the bar code symbology is supported and
configured correctly

m2000sp G-series Service Manual 7-25


200942-103—September 2007
Troubleshooting Section 7

• Check that the carrier is located in the correct grid


location
• Check that the rack is enabled
• If you suspect a hardware issue, refer to PosID Hardware on
page 7–44

Failed to Detect a Previously Scanned Carrier


• Check that all carriers are in the correct locations on the
worktable
• Check that no carriers have moved from the location of
their previous scan
• Verify the PosID is not hitting the racks when moving
• Replace the rack
• If you suspect a hardware issue, refer to PosID Hardware on
page 7–44

Doorlocks (Error Codes 3502-3599)

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

Additional Doorlock Errors


• Check that the door is completely closed and nothing is
obstructing the doorlocks
• Check for damage to the doorlock fingers
• Cycle power on the SCC and the instrument
• Retry the process
• If you suspect a hardware issue, refer to Doorlocks
Hardware on page 7–36

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Section 7 Error Codes

Fast Wash Pump (FaWa) (Error Codes 3602-3699)

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

Additional FaWa Errors


• Cycle power on the SCC and the instrument
• Retry the process
• If you suspect a hardware issue, refer to FaWa Hardware on
page 7–37 or Dilutor Hardware on page 7–34

Heaters (Error Codes 3701-3799)

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

Additional Heater Errors


• Check all cable connections to the heater blocks
• Cycle power on the SCC and the instrument
• Retry the process
• Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• If you suspect a hardware issue, refer to Heater Hardware
on page 7–38

SMIO (Smart Monitored Input/Output) (Error Codes 3802-3899)

Invalid Command/Operand
• Cycle power on the SCC and the instrument
• Retry the process

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200942-103—September 2007
Troubleshooting Section 7

Additional Errors
• Check cable connections at the SMIO board and at the
SCC
• If you suspect a hardware issue, refer to SMIO/SMIO-SAFY
Hardware on page 7–58

Output Deck Chiller (Error Codes 3900-3949)

Communication Errors
• Check the seating of the power cable at the chiller
• Check the Edgeport™ cable connections
• Cycle power on the chiller
• Unplug and replug the Edgeport
• If you suspect a hardware issue, refer to Output Deck
Hardware on page 7–44
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37

Temperature Errors
• Check all cable connections between the chiller and the
m2000sp instrument
• Check the Edgeport cable connections
• Cycle power on the chiller
• Unplug and replug the Edgeport
• Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• If you suspect a hardware issue, refer to Output Deck
Hardware on page 7–44
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37

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200942-103—September 2007
Section 7 Error Codes

Hood (Error Codes 3950-3999)

Incompatible Hardware
Use the hardware configuration screen to reconfigure a
G-Series as an E-Series.

Signal Tower
• Check cable connections
• Cycle power to SCC and instrument
• Verify that the signal tower is implemented in the
Hardware Configuration screen
• If you suspect a hardware issue, refer to Edgeport™
Hardware on page 7–37 or Signal Tower Hardware on page
7–59

Disposable Tip Inventory (Error Codes 7000-7099)


• Cycle power on the SCC and the instrument
• Retry the process
Many Disposable Tip Inventory error codes are related to
actions during processing. Action is not required for these
error codes.

User Notification (Error Codes 7100-7199)


User notification codes are informational in nature and
require no action.

Application Specification (Error Codes 7200-7299)


• Install all required Liquid Class Files
NOTE: If installing or updating a liquid class, all
application files that reference that liquid class
must be deleted and re-installed
• Delete the Application Specification
• Reinstall the Application Specification

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200942-103—September 2007
Troubleshooting Section 7

If you continue to experience errors, there might be a problem


with the underlying Application Specification file. Contact
GSS for Application Specification support.

Result Management (Error Codes 7300-7399)

General Errors
For most result management errors, you will need to reprocess
the samples.

Exporting Errors
• Try exporting the Test Order files again
• Retry export with a different CD
• “Network” might not be available as an export option;
configure m2000rt computer name and check cabling
• Check the network cable at the m2000rt™ and m2000sp
• Cycle power on the SCC
• Replace the CD
• If you suspect a hardware issue, refer to SCC Hardware on
page 7–54

Backup and Restore (Error Codes 7400-7499 / 8700-8799)

Backup/Restore Failed
• Open/close CD drive door, wait 12 seconds, and try again
• Replace the CD
• Cycle power on the SCC
• Retry the procedure
• Check the logs for information
• Ensure that all non-application windows are closed
behind the application window
• If you suspect a hardware issue, refer to SCC Hardware on
page 7–54

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200942-103—September 2007
Section 7 Error Codes

Database Failure
First, retry the procedure. The database that is being backed
up/restored might be incompatible with the current software
version. Contact GSS to see if there is any way the database
can be read.
The majority of these error codes relate to actions during
processing (completion of backup, invalid state, etc.). Follow
any necessary prompts for these actions.

Shared Application Specification (Error Codes 7600-7749)


Application Specification errors are due to fundamental errors
in the Application Specification file. Contact GSS for
Application Specification support.

Network Data Store (Error Codes 7750-7799)


• Check network cable connections at the router, SCC, and
data store
• Check that the data store computer is turned on
• Check power to all components
• Check network configuration
• If you suspect a hardware issue, refer to Network Hardware
on page 7–59
• If you suspect a configuration issue, refer to Network
Configuration Screen on page 4–170 of Section 4

Report Requests (Error Codes 7900-7999)

Report Failed Due to Software


• Cycle the power on the SCC
• Retry report generation

Report Failed Due to Printer


• Cycle the power on the printer and retry report generation

m2000sp G-series Service Manual 7-31


200942-103—September 2007
Troubleshooting Section 7

• Verify that correct printer is being used (Okidata 4350)


• Verify that the printer is set to default (via Control Panel)
• Check that cable connections are tight
• Check that the printer status is Ready
• If you suspect a hardware issue, refer to Printer Hardware
on page 7–48

User Access (Error Codes 8000-8049 / 8400-8499)


The majority of these errors codes are user notification
prompts. Follow the instructions given on the prompts.

Network Configuration (Error Codes 8050-8099)

Configuration Screen Errors


These errors relate to actions during network configuration,
and occur while in the network configuration screen. Follow
the screen prompts to resolve these errors.

Test Failed
• Check crossover cable connections at router and SCC
• Check power to components (SCC and router)
• Check cable type
• If you suspect a hardware issue, refer to Network Hardware
on page 7–59
• If you suspect a configuration issue, refer to Network
Configuration Screen on page 4–170 of Section 4

Audible Alert (Error Codes 8100-8299)


The majority of these errors codes are due to user-entry error.
Follow the instructions given on the prompts.

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200942-103—September 2007
Section 7 Error Codes

Date/Time Configuration (Error Codes 8500-8599)


The majority of these errors codes are due to user-entry error.
Follow the instructions given on the prompts.

Serial Number (Error Codes 8600-8699)


The majority of these errors codes are due to user-entry error.
Follow the instructions given on the prompts.

Operating System (Error Codes 8800-8899)


Operating system error codes are informational in nature.

Language and Locale (Error Codes 8900-8910)


These errors relate to actions during Language and Locale
configuration, and occur while in the configuration screen.
Follow the screen prompts to resolve these errors. Refer to
Language and Locale on page 4–188 of Section 4.

On-line Documentation (Error Codes 8911-8950)


• Retry opening document
• Verify that the correct level of user access is logged in
• Reload on-line documentation

Architecture (Error Codes 9000-9099)


For architecture error codes, cycle power on the SCC.

m2000sp G-series Service Manual 7-33


200942-103—September 2007
Troubleshooting Section 7

Hardware Errors

The following section contains general information on all


hardware error codes for the m2000sp system, as well as a series
of possible troubleshooting steps to resolve each error. The
hardware subsystems have been alphabetized.
IMPORTANT: Be sure to verify the instrument you are
! troubleshooting is a G-series and not an E-series model.
(For E-series model troubleshooting, see the m2000sp
E-series Service Manual.)

CAN BUS Hardware

In S&S:
• Verify that under <Options>,<SMIO Panel
Configuration type>, the device is listed as NAT systems
• Check the information panel to ensure that all devices are
available (<Instrument>,<Basic Set-up>,<Information>)

Check the following and replace if necessary:


• Status LEDs on the hardware assemblies for fatal hardware
errors (e.g., both lights lit on the PosID)
• Unplug all options connected to the Option CAN BUS one
at a time to determine what is causing the bus to be
blocked; replace hardware as needed

Dilutor Hardware

Plunger Overload Errors


Typically, this problem requires a dilutor replacement;
however, a clog or kink in the fluidics could cause plunger
overload, as well. Therefore, first check the following and
replace as needed:
• Valve
• Syringe
• Tubing extension
• Dispense tubing

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200942-103—September 2007
Section 7 Hardware Errors

One Dilutor Failing


• In S&S:
– Enter the single line command DNREE to get an error
code response for a particular dilutor, where N is the
dilutor firmware address 0-7
– Use the LiHa dilutor tool to verify dilutor
functionality
• Check the following and replace if necessary:
– Green LED blinking
– Syringe movement is unobstructed
– Dilutor is firmly plugged into the VCC Dilback board
– Dilutor address set correctly
– Check that tubing is unkinked
– Check that the valve is unblocked
– Check for over- or under-tightening of the
valve/syringe
– Valve
– Syringe
– Dilutor

All Dilutors Failing


• In S&S:
– Use the LiHa Dilutor tool to verify dilutor
functionality
• Check the following and replace if necessary:
– Fuse FU4 on the OPTIBO board
– Cable connection between VCC Dilback board and
OPTIBO board
– Cables
– VCC Dilback board
– OPTIBO board

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200942-103—September 2007
Troubleshooting Section 7

Doorlocks Hardware

One Doorlock Failing


• In S&S:
– Check door lock functionality using <Options>,
<Access Status Options>
• Check the following and replace if necessary:
– Door latch alignment with door lock
– Cable connection between door lock and SMIO-SAFY
board
– If the right lock is failing, check the status of the left
(the left lock must lock first)
– Swap cables to determine status of the board
– Swap SMIO board with SMIO-SAFY board (address,
jumpers, firmware)
– Doorlocks

Both Doorlocks Failing


• In S&S:
– Check door lock functionality using <Options>,
<Access Status Options>
• Check the following and replace if necessary:
– Fuse FU1 on the OPTIBO board
– Jumper on SMIO-SAFY board is set to J9, outer two
pins
– Address on SMIO-SAFY board is set to 1
– Cable connection between SMIO-SAFY board and
OPTIBO board (Options 6-J18)
– Cables
– SMIO-SAFY board

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200942-103—September 2007
Section 7 Hardware Errors

Edgeport™ Hardware

In Explorer:
• Verify that Port 1 is set to COM3 in the Edgeport
Configuration menu

Check the following and replace if necessary:


• The LED on the front of the Edgeport is blinking
(primarily green); if not, disconnect the USB cable, wait
five seconds, and reinsert the USB cable
• Cable connection from the SCC to the Edgeport
• Cycle power on the SCC
• Cable connection from Edgeport to chiller
• Edgeport USB must be plugged into the same USB port as
it was initially (back, lower left)
• RS-232 cable for hood (if installed) must be plugged into
Port 2 of the Edgeport

FaWa Hardware

In S&S:
• Perform FaWa Fill/Flush Protocol on page 4–122 of Section
4
• Perform FaWa Pump Test on page 4–123 of Section 4

Check the following and replace if necessary:


• Fuse FU6 on the OPTIBO board
• Check that tubing is unkinked
• Check for tightness of the tubing connections
• Verify that there is sufficient system liquid
• Cable connection between MPO board and OPTIBO board
(CAN Opt 1 – FaWa)
• Pressure relief valve
• FaWa pump
• MPO board
• Cables

m2000sp G-series Service Manual 7-37


200942-103—September 2007
Troubleshooting Section 7

• OPTIBO board

Heater Hardware

One Heater Failing


• In m2000sp Application:
– Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• Check the following and replace if necessary:
– Fuses FU8 and FU9 on the OPTIBO board
– Cable connection between the OPTIBO board and the
1 mL Subsystem (CAN Opt 3 and 4)
– Internal cable connection between the heater block
and MBS print complete for Mags board
– Address settings
– Heater block
– Internal cable
– External cable
– OPTIBO board

Both Heaters Failing


• In m2000sp Application:
– Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• Check the following and replace if necessary:
– Fuses FU8 and FU9 on the OPTIBO board
– Check for over/under tightening of cable connections
– Cable connection between the OPTIBO board and the
1 mL Subsystem (CAN Opt 3 and 4)
– Cables
– OPTIBO board

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Section 7 Hardware Errors

LiHa Hardware

Shortened X-drive Travel (Arm not moving all the way to


the right)
• Check whether an E-series X-drive was installed by
mistake
• If so, replace it with the correct G-series X-drive (PN
619854)

No Response
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Verify the Device M1 is known on the Firmware
Download page (if not, download LiHa firmware)
– Enter the single line command A1REE
– The response should be A1@@@@@@@@@@@ if
all axes are responding correctly
– If errors are received, address the specific axis
involved by following the steps in the
appropriate section below
• Check the following and replace if necessary:
– Indicator light on Genesis CU board (if out, the board
needs to be replaced)
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Cable connection between the X-drive assembly and
the LiHa backplane board
– Fuse FU2 on the OPTIBO board
– Cables
– OPTIBO board
– LiHa backplane board

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200942-103—September 2007
Troubleshooting Section 7

LiHa X-axis
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Enter the single line command A1REE
– The response should be A1N@@@@@@@@@@,
where N is the axis error code
– Check Y-spread
– Check LiHa reference positions
– Check instrument scaling
• Check the following and replace if necessary:
– Look for obstructions that would prevent proper
initialization of the LiHa
– Fuse FU2 on the OPTIBO board
– Cable connection between the X-drive assembly and
the LiHa backplane board
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Verify the distance between the X-drive and the X-gap
is 2.0 mm
– X-drive belt
– X-drive assembly
– Cables
– Servo power board
– Device CU board
– LiHa backplane board
– LiHa

LiHa Y-axis or Ys-axis


• In S&S:

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200942-103—September 2007
Section 7 Hardware Errors

– Verify that the LiHa is recognized on the Basic Setup


Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Enter the single line command A1REE
– The response should be A1@N@@@@@@@@@
for Y or A1@@N@@@@@@@@ for Ys, where N is
the axis error code
– Check Y-spread
– Check LiHa reference positions
– Check instrument scaling
• Check the following and replace if necessary:
– Check Z-rod perpendicularity to the worktable
– check for any Z-rod binding in movement
– clean the Z-rods
– Check belt tension
– Check ball bearings
– Check screws between the axles and pulleys
– Cable connection between the Y-motors and the LiHa
backplane board
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Cables
– Device CU board
– Y-belt
– Y-motors
– LiHa backplane board
– OPTIBO board

LiHa Z-axis
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab

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200942-103—September 2007
Troubleshooting Section 7

– Verify that each LiHa axis is present and OK on the


LiHa informational panel
– Enter the single line command A1REE
– The response should be A1@@@N@@@@@@@,
where N is the axis error code
– Check Z-axis setup and offset
– Check tip type length
• Check the following and replace if necessary:
– Check screws between the axles and motors
– Cable connection between the motor and the LiHa
backplane board
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Swap or replace the servo boards (2 through 5) and
change the addresses:
– Servo 2 (Z3 and Z6)
– Servo 3 (Z7 and Z2)
– Servo 4 (Z1 and Z8)
– Servo 5 (Z5 and Z4)
– Cables
– LiHa backplane board
– OPTIBO board

DiTi Ejection
• In S&S:
– Verify that the LiHa is recognized on the Basic Setup
Information Tab
– Verify that each LiHa axis is present and OK on the
LiHa informational panel
– Check Each of the Tip Adapters on page 4–58 of
Section 4
– Calibrate the Lower DiTi Eject on page 4–63
– Perform the Lower DiTi Eject Test on page 4–64

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200942-103—September 2007
Section 7 Hardware Errors

– Check the Set Discard distance by entering AIRST at


the single line command
– The response should be A1@___,___,45
– If the response is different, set the distance by
entering A1SST,45, then M1SOW
• Check the following and replace if necessary:
– Clean the linkage pin and holes at the lever and yoke
on the linkage mechanism, and lubricate the pin
– Perform the Rocker Adjustment Procedure on page
9–71 of Section 9
– Check that the thin lever on the solenoid lies in the
center of the yoke at a 90 degree angle to the drive
shaft
– Cable connection between the Lower DiTi Eject
Option and the LiHa backplane board
– Cable connection between LiHa backplane board and
OPTIBO board (LiHa J5 – LiHa)
– Tip adapters
– Solenoid
– Cables
– ILID cable
– ILID board
– LiHa backplane board
– DC Servo Board Z5/Z4 position 5
– LiHa

Tip Adapters
• In S&S:
– Perform the Tip Adapter Test (refer to Check Each of
the Tip Adapters on page 4–58)
• Check the following and replace if necessary:
– The straightness and alignment of the tip adapter
– The set screw on the Z-rod

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200942-103—September 2007
Troubleshooting Section 7

Output Deck Hardware

Chiller
• In m2000sp Application:
– Perform the Chiller and Heater Temperature Check on
page 5–52 of Section 5
• Check the following and replace if necessary:
– Power switch on the back of the chiller is ON
– Power cord is firmly plugged into the back of the
chiller
– The RS-232 cable between the chiller and the
Edgeport™ is connected to Port 1 on the Edgeport

Output Deck Support


If experiencing difficulty in setting the master mix block into
the back of the Output Deck support, check the ball bearing
detent on the Output Deck support. This can be adjusted as
necessary.

PosID Hardware

Bar Code Reading


• In S&S:
– Confirm the PosID 2 parameters (PosID mechanical
accuracy and scanner head mechanical accuracy)
• Check the following and replace if necessary:
– The quality of the bar codes
– The orientation and positioning of the bar codes
– The integrity and positioning of the frogs
– Clean the Bar Code Reader window
– Cable connection from scanner to Y/B-board cable
– Cable connection from DSP board to Y/B-board cable
– Cable connection from X-flex cable to Y/B-board and
PosID board

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200942-103—September 2007
Section 7 Hardware Errors

– Cable connection from Y/B-cable to the PosID board


and the PosID CU board
– Cable connection from the PosID CU board to the
OPTIBO board (CAN Opt2-CU PosID)
– Scanner
– DSP board
– Y/B-board
– B-motor
– Cables
– PosID CU board
• In m2000sp Application:
– Confirm the PosID 2 bar code settings in the m2000sp
software
– bar code type
– length
– check digit option
– start-stop character

Tube Sensing
• Check the following and replace if necessary
– Clean the tube sensors
– Cable connection between the PosID CU board and
PosID board
– Cable connection between the OPTIBO board (CAN
Opt 2-CU PosID) and the PosID CU board
– Cable connection of the X-flex cable between the
PosID board and PosID 2 module
– Cable connection between the NO Tube Sensor and
the Y/B board
– No Tube Sensor
– Y/B board
– Servo board
– Y/B cable

m2000sp G-series Service Manual 7-45


200942-103—September 2007
Troubleshooting Section 7

– X-flex cable
– PosID CU board

PosID 2 X Movement
• In S&S:
– Enter the single line command P1REE
– The response should be N@@, where N is the
axis error code
• Check the following and replace if necessary
– Manually check for free movement of the PosID 2
– Cable connection between the X-motor and PosID
board
– Cable connection between the PosID CU board and
the PosID board
– Check the X-belt tension
– Cable connection of the X-flex cable between the
PosID board and PosID 2 module (Y/B board)
– Cable connection between the OPTIBO board and the
PosID CU board (CAN Opt 2 – PosID)
– Fuse FU7 on the OPTIBO board
– Cables
– X-servo board
– X-motor
– X-drive assembly
– Belt
– PosID board
– OPTIBO board

PosID 2 Y-movement
• In S&S:
– Enter the single line command P1REE

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200942-103—September 2007
Section 7 Hardware Errors

– The response should be @N@, where N is the


axis error code
• Check the following and replace if necessary
– Verify the gap between the release finger of the PosID
2 and worktable surface equals 0.2 mm
– Verify the gap between the PosID Service Rack and
gripper pin equals 0.5 mm
– Cable connection between the PosID CU board and
the PosID board
– Check the Y-belt tension
– Cable connection of the X-flex cable between the
PosID board and PosID 2 module (Y/B board)
– Cable connection between the OPTIBO board and the
PosID CU board (CAN Opt 2 – PosID)
– Fuse FU7 on the OPTIBO board
– Check the tube sensor cable for interference with the
Y-slide
– Cables
– Y-belt
– Y-motor
– Servo board
– Y/B board
– Gripper assembly
– Y/B cable
– X-flex cable
– PosID CU board

Power Supply

Check the following and replace if necessary:


• Power switch is on
• Power is available at the outlet (breaker on)
• Green light by the power supply is illuminated
• Power interlock switch is in place

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200942-103—September 2007
Troubleshooting Section 7

• Power supply leads to the OPTIBO are securely screwed in


place
• 24V are available at the OPTIBO

Printer Hardware

Check or do the following:


• Power switch is on
• Replace the USB cable with a parallel printer cable (doing
so will create a copy of the printer in the printer menu);
then, set the copy to be the default printer

<Image Drum Missing> Message


• Replace the printer

RoMa Hardware

Shortened X-drive Travel (Arm not moving all the way to


the right)
• Check whether an E-series X-drive was installed by
mistake
• If so, replace it with the correct G-series X-drive (PN
619854)

No Response
• In S&S:
– Verify that the RoMa is recognized on the Basic Setup
Information tab
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Verify that Device C1 is known on the Firmware
Download page (if not, download RoMa firmware)
– Enter the single line command C1REE
– The response should be C1@@@@@ if all axes
are responding correctly

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200942-103—September 2007
Section 7 Hardware Errors

– If errors are received, address the specific axis


involved by following the steps in the
appropriate section below
– If no errors are received, run a random move
test to see if any errors occur then
• Check the following and replace if necessary
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board (Arm R1 J15-RoMa)
– Fuse FU3 on the OPTIBO board
– CU board

RoMa Rotator
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Verify that Device C1 is known on the Firmware
Download page (if not, download RoMa firmware)
– Enter the single line command C1REE
– The response should be @@@N@, where N is
the rotator error code
– Perform the Reference Plate Test on page 4–79 of
Section 4, and auto-adjust the rotator if necessary
– After reperforming the Reference Plate Test, perform
Manual RoMa Rotator Adjustments on page 4–83 if
necessary
• Check the following and replace if necessary
– Manually rotate the gripper head, and check for
bearing roughness
– Cable connection between the rotator motor and
gripper head
– Cable connection between the gripper board and the
RoMa 2 backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Common servo boards (swap to check for board
failures); check board address settings

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200942-103—September 2007
Troubleshooting Section 7

– Rotator motor
– Y/R servo board
– RoMa 2 backplane board
– Gripper board
– RoMa Freedom backplane board
• In m2000sp Application:
Run any of the RoMa alignment M&D checks to verify
correction rotational alignment of the RoMa (refer to
RoMa Checks on page 5–27 of Section 5). Manually align
the RoMa rotator if necessary.

RoMa Gripper
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Enter the single line command C1REE
– The response should be @@@@N, where N is
the gripper error code
– Check the gripper setup in <RoMa>,<Set Type>
– Perform the Reference Plate Test on page 4–79 of
Section 4, and auto-adjust the rotator if necessary
• Check the following and replace if necessary
– Manually rotate the gripper head, and check for
bearing roughness
– Use the move panel to move the gripper fingers above
a straight surface (the reference teach plate, for
example) to verify the fingers are parallel and even
– Use the move panel to move the gripper fingers above
the 1 mL Subsystem and bring them down to the
surface to verify that the bottoms of the pads are flat
to the surface; readjust the screws if necessary
– Cable connection between the gripper motor and
gripper board
– Cable connection between the gripper board and the
RoMa 2 backplane board

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200942-103—September 2007
Section 7 Hardware Errors

– Cable connection between RoMa 2 backplane board


and the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Z/G servo board
– RoMa 2 backplane board
– Gripper board
– RoMa Freedom backplane board
– RoMa
– Gripper fingers

RoMa Y-axis
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Enter the single line command C1REE
– The response should be @N@@@, where N is
the Y-axis error code
– Check the scaling factor
– Initialize the axis and check for extended errors
• Check the following and replace if necessary
– Manually check for free movement of the RoMa arm
– Y-belt tension
– Screws between the axles and the pulley
– Cable connection between the Y-pulley and the
RoMa 2 backplane board
– Cable connection between the RoMa 2 backplane
board and the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Y-belt
– Servo board
– Y-motor

m2000sp G-series Service Manual 7-51


200942-103—September 2007
Troubleshooting Section 7

– Cables
– RoMa 2 backplane board
– RoMa Freedom backplane board
– RoMa

RoMa Z-axis
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
– Enter the single line command C1REE
– The response should be @@N@@, where N is
the Z-axis error code
– Check the RoMa range
– Initialize the Z-axis and check for extended errors
• Check the following and replace if necessary
– Manually check for free movement of the RoMa arm
– Grease the worm gear
– Cable connection between the Z-motor and the
RoMa 2 backplane board
– Cable connection between the RoMa 2 backplane
board and the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Z-motor
– Servo board
– Cables
– RoMa 2 backplane board
– RoMa Freedom backplane board
– RoMa

RoMa X-axis
• In S&S:

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200942-103—September 2007
Section 7 Hardware Errors

– Verify that each RoMa axis is present and OK on the


RoMa information panel
– Enter the single line command C1REE
– The response should be N@@@@, where N is
the X-axis error code
– Check the LiHa reference positions
– Check the scaling factor
– Initialize the X-axis and check for extended errors
• Check the following and replace if necessary
– Manually check for free movement of the RoMa arm
– Fuse FU3 on the OPTIBO board
– Cable connection between the X-drive assembly and
the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– X-belt
– X-belt assembly
– Cables
– Servo power board
– Device CU board
– RoMa Freedom backplane board
– RoMa

RoMa Z-brake
• In S&S:
– Verify that each RoMa axis is present and OK on the
RoMa information panel
• Check the following and replace if necessary
– Clean the Z-brake
– Check the Z-brake for cracks
– Verify the distance between the brake shoe and
Z-brake is 7.25 mm +0.2mm; adjust if necessary

m2000sp G-series Service Manual 7-53


200942-103—September 2007
Troubleshooting Section 7

– Cable connection between the Z-brake and the


RoMa 2 backplane board
– Cable connection between the RoMa 2 backplane
board and the RoMa Freedom backplane board
– Cable connection between RoMa Freedom backplane
board and the OPTIBO board
– Z-brake
– Servo board
– RoMa 2 backplane board
– Cables
– RoMa Freedom backplane board
– RoMa

SCC Hardware

Communication Errors
• Check the following and replace if necessary
– Cable connection between the 1 mL Subsystem and
CAN options 3 and 4
– Fuse FU1 on the OPTIBO board
– Cable connection between SCC and the OPTIBO
board (Host RS-232)
– Check that the port setting is set to COM1
– RS-232 cable
– Power cable
– OPTIBO board
– CU board
• After a short-term m2000sp shutdown, cycle SCC power

No Display
• Check monitor connections to SCC
• Check that power switches are in the on position (one in
front and one in rear next to power cord)

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200942-103—September 2007
Section 7 Hardware Errors

Long Bootup
It is normal for networked systems to take an additional 60
seconds to bootup as they acquire the network.

No Boot-up or Login Screens


Replace the hard drive if you experience any of the following:
• Black screen with blinking cursor in upper left corner
• Blue screen with no Login screen after several minutes
• Constant Yellow light, hard drive is slow to respond

No Green Light After Power Switch Is Depressed


• Check for fan operation in the rear
– If on, switching circuit is bad, replace power supply.
– If not operating, check input fuse
• If good, power supply is defective
• If fuse is blown, secure power on the SCC and
replace fuse; if SCC still fails to power up
replace power supply

Noisy Hard Drive


Replace the hard drive if you experience any of the following:
• High-pitched whine, indicating the bearings or motor is
going bad
• Clicking noises, indicating the read heads are skipping

CD Drive Not Reading Files


• Try exiting out of the current application and re-entering
to reconnect with the CD drive
– If this does not work, replace the CD drive
• Replace the CD drive if the drive does not respond to the
“Open” command

Memory Problems
• If the RAM buffer is out of available memory, restart the
SCC to clear the buffer

m2000sp G-series Service Manual 7-55


200942-103—September 2007
Troubleshooting Section 7

• If the SCC constantly locks up when operating instrument


protocols, there might not be enough memory or the
buffer is at maximum capacity
– Restart the SCC

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200942-103—September 2007
Section 7 Hardware Errors

Sensor Hardware
Work through the following Problem Solving Tree to resolve
sensor problems:

m2000sp G-series Service Manual 7-57


200942-103—September 2007
Troubleshooting Section 7

SMIO/SMIO-SAFY Hardware

In S&S:
• Check board function using <Options>,<SMIO for NAT>
and <Options>,<Access Status Options>

Check the following and replace if necessary:


• All board to sensor connections
• The cable between the SMIO and the SMIO-SAFY boards
• The cable between the SMIO-SAFY board and the OPTIBO
board
• Check the address on the boards (SMIO-SAFY = 1,
SMIO = 2)
• Jumper connections on J9 (Inside 2 pins on the SMIO
board, outside 2 pins on the SMIO-SAFY board)
• Swap the boards for troubleshooting (change address,
jumpers, and firmware)
• SMIO board
• SMIO-SAFY board
• Cables

Instrument Connection Issues


• Reseat the cable connection at the SCC
• While powering the instrument off and then back on,
perform a system shutdown and restart on the SCC
• Set COM 1 port in GenDRV (necessary when a previously
installed modem is removed)
– Launch pcAnywhere
– Select modem
– Select delete
– Launch S&S
– Navigate to the GenDRV (minimized window)
– Select <Options>,<Driver>
– Set the port to COM 1, select <OK>
– Restart the SCC

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200942-103—September 2007
Section 7 Hardware Errors

Database/Restore Issues
• Shutdown the SCC (particularly if you observe a red X or
system lock up)
• Restart the SCC
• Login as FSE
• Navigate to the Database Manager
• Place the Backup CD in the drive
• Wait until the CD light turns off (approximately
12 seconds)
• Select restore

Network Hardware
In m2000sp Application:
• Check that the network is correctly configured
Check the following and replace if necessary:
• Network cable at router is connected to LAN jack
• Network Data Store computer is connected to LAN jack
• Router should be powered on and blinking green
• SCC is connected to router

Signal Tower Hardware


In m2000sp Application:
• Check that the signal tower is configured correctly and
test (refer to Signal Tower on page 4–186)
Check the following and replace if necessary:
• Cable connection from the SCC to the Edgeport
• Cable connection from the Edgeport to the hood
controller board
• LED on the hood controller board should be green

m2000sp G-series Service Manual 7-59


200942-103—September 2007
Troubleshooting Section 7

NOTES

7-60 m2000sp G-series Service Manual


200942-103—September 2007
Section 8: Maintenance

SECTION 8:
MAINTENANCE

m2000sp G-series Service Manual 8-1


200942-103—September 2007
Maintenance Section 8

NOTES

8-2 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 Table of Contents

Table of Contents
TOC
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-5
Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-6
Cleaning Agents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-6
Clean Liquid System Procedures . . . . . . . . . . . . . . . . . . . . . 8-8
Microbial Growth Inspection . . . . . . . . . . . . . . . . . . . . . 8-8
Clean the System Liquid Container Procedure . . . . . . . 8-8
ProClin® Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-10
Clean the Liquid Waste Container . . . . . . . . . . . . . . . . 8-10
RoboScrub Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . 8-12
Gas Spring Maintenance Check . . . . . . . . . . . . . . . . . . . . . 8-14
Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-15
At Each Site Visit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-15
Biannual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-15
Annual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-17
m2000sp G-series Biannual Preventive Maintenance Checklist
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-19
m2000sp G-series Annual Preventive Maintenance Checklist . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-21

m2000sp G-series Service Manual 8-3


200942-103—September 2007
Maintenance Section 8

Introduction
This section summarizes the biannual and annual preventive
maintenance procedures. Maintenance must be performed as
described in this manual. For optimum performance and
reliability of the instrument, routinely perform the
maintenance and cleaning tasks described in this chapter.
IMPORTANT: Be sure to verify the instrument you are
! maintaining is a G-series and not an E-series model.
(For E-series model maintenance information, see the
m2000sp E-series Service Manual.)

Use only original m2000sp accessories, consumables, and


G-series field replaceable units. If other parts are used during
the normal warranty period, the manufacturer’s guarantee
could be invalidated. Modifications should be made by an
Abbott- authorized representative only. Abbott Molecular will
not accept responsibility for any claim resulting from
unauthorized modification or alteration of the m2000sp.
CAUTION: Personal Injury Risk. Take care to avoid
injury when using sharp tools.
• Do not direct tool points, such as the blade of a
screw driver, toward the face, body, or hands.
• If cutting of tubing, etc., is required, NEVER cut in
the direction of the face, body, or hands.

8-4 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 Safety

Safety
Observe the following safety notices.

WARNING: Potential Biohazard. Indicates an area


where the FSE or operator may be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.

CAUTION: Chemical Hazard. The FSE or operator may


be exposed to hazardous chemicals when handling
Abbott Molecular sample preparation reagents. The
exposure to hazardous chemicals is minimized by
following instructions provided in product-specific
labels and product-specific Material Safety Data Sheets
(MSDS). Wear appropriate personal protective
equipment, such as gloves, lab coat, and protective
eyewear.
WARNING: Electrical Shock Hazard. When cleaning
the instrument surfaces (e.g., worktable, instrument
panels, etc.) or performing maintenance tasks, always
power off the instrument first.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. While working with the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Keep back from the worktable surface during these
procedures.
• Fast moving devices could cause injuries.

m2000sp G-series Service Manual 8-5


200942-103—September 2007
Maintenance Section 8

Consumables
Detailed cleaning instructions are located in Section 8, Service
and Maintenance of the m2000sp Operations Manual, which
is available for review within the m2000sp Software under the
<Help> menu.

Cleaning Agents

Alcohol
Use ethanol or 2-Propanol (Isopropanol).

Bleach
Use 0.1% or 0.5% sodium hypochlorite solution (2% or
10% chlorine bleach) depending on the task to be performed.
WARNING: Do not mix any oxidizing agents such as
straight household bleach, or any solution that
contains sodium hypochlorite in percentages higher
than 0.5%, with the mLysis, mWash 1, mMicroparticles,
mLysisDNA, mWash 1DNA, or the mMicroparticlesDNA
reagents in the Abbott sample preparation reagent
pack. Do not mix any oxidizing agents such as sodium
hypochlorite with the liquid waste of the m2000sp.
Toxic gases could be generated from these mixtures.
Pressure can build up in the closed container of the
mixtures. It is the responsibility of each facility to
characterize its waste and ensure it is handled and
disposed of in accordance with local, state, and federal
regulations.

Water
Use distilled or deionized water.
CAUTION: Strong detergents may dissolve carrier and
worktable surface coatings.
• Use only recommended cleaning agents.
• Chlorine solution can pit equipment and metal.
Use adequate amounts or repeated applications of
70% ethanol or water until chlorine residue is no
longer visible.

8-6 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 Consumables

Detergent Solutions
• Sporicidin Brand Disinfectant Solution (pump spray and
refill) EPA Reg.: 8383-3; Sporicidin International,
Rockville, MD
• Sporicidin Brand Disinfectant Towelette EPA Reg.: 8383-7;
Sporicidin International, Rockville, MD
• RoboScrub: contact Abbott Molecular GSS for assistance in
ordering RoboScrub.
• ProClin® 300 Preservative (product number 48914-U)
can be purchased globally from the Sigma-Aldrich
company’s website at www.sigmaaldrich.com

m2000sp G-series Service Manual 8-7


200942-103—September 2007
Maintenance Section 8

Clean Liquid System Procedures

Microbial Growth Inspection


Microbe-free distilled or deionized water must be used in order
to minimize the risk of microbial contamination of the liquid
handling system.
Inspection for microbial growth should be performed at each
site visit, as follows:
1. Visually inspect the inside of the system liquid container
and the outside of the cap tubing for areas of discoloration
(brownish-yellow or blue-green spots).
2. Even if nothing is visually present, move a gloved finger
along the supply tubing. If the glove feels slippery after
removal from the tubing, biofilm is likely present.
If no signs of biofilm are found, no further action is required.
If biofilm is found or suspected:
1. Perform the Clean the System Liquid Container Procedure
below
2. Replace all system tubing (refer to Tubing System on page
9–174 of Section 9)
3. Inform the customer of the need to use ProClin® 300
Preservative to prevent microbial growth
4. With the customer’s permission, perform the ProClin®
Procedure on page 8–10

Clean the System Liquid Container Procedure


1. Open the front-left door of the cabinet.
2. Pull the waste tray forward.
3. Disconnect the white connector from the top of the
system liquid container by holding the tubing and
pressing in on the quick-connect fitting.
4. Wrap the tubing with absorbent material.
5. Remove the system liquid container from the tray.
6. Remove the cap from the container and drain the
remaining system liquid.

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200942-103—September 2007
Section 8 Clean Liquid System Procedures

7. Rinse the system liquid container and the inside of the


container tubing with 0.1% sodium hypochlorite
solution.
8. Rinse the system liquid container with distilled or
deionized water a minimum of five times to ensure that all
bleach is removed.
9. Wipe the tubing on the underside of the cap with a
lint-free cloth or paper towel moistened with 0.1% sodium
hypochlorite solution.
10. Rinse the tubing on the underside of the cap with distilled
or deionized water or wipe with a lint-free cloth or paper
towel moistened with distilled or deionized water. Ensure
that all bleach residue has been removed.
11. Refill the system liquid container with distilled or
deionized water.
NOTE: Perform the ProClin® Procedure on page
8–10 if ProClin is used by the customer.
12. Replace the cap on the system liquid container and return
the container to the waste tray.
13. Reconnect the tubing on the system liquid container by
pressing the tubing with quick-connect fitting onto the
connector on the cap. Ensure the fitting and connection
are secure.
14. Push the waste tray back into the cabinet.
15. Close the front-left door of the cabinet.
16. Perform the Extensive Flush on page 5–10 of Section 5 if
this procedure is performed at any time other than during
Daily Maintenance.

m2000sp G-series Service Manual 8-9


200942-103—September 2007
Maintenance Section 8

ProClin® Procedure
1. Remove the cap from the system liquid container, empty
whatever liquid remains, and refill the container with 2.5
liters of distilled or deionized water.
2. Volumetrically add 3.5 mL of ProClin® 300 to the water in
the container.
CAUTION: Chemical Hazard. ProClin 300
Preservative is a corrosive chemical. Refer to its
MSDS, laboratory guidelines, and local
government regulations for instructions on proper
handling and disposal. Wear appropriate personal
protective equipment such as gloves, lab coats,
and protective eyewear.
NOTE: Serological pipettes or any other
volumetric measurement device with an accuracy
better than + 0.2 mL can be used.
3. Fill the container with an additional 7.5 liters of distilled
or deionized water (for a total of 10 liters).

Clean the Liquid Waste Container


WARNING: Potential Biohazard. Instrument waste
could be contaminated with potentially infectious
materials.
• Observe basic biohazard precautions
• Wear appropriate personal protective equipment such
as gloves, lab coats, and protective eyewear

Dispose of the liquid waste and the guanidine-containing


Abbott sample preparation reagents in accordance with local,
state, and federal regulations.

8-10 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 Clean Liquid System Procedures

WARNING: The level of guanidine in the liquid waste


container can range from 0.5 to 1.6 M. Do not mix any
oxidizing agents such as sodium hypochlorite with the
sample preparation reagents containing guanidine
(mLysis, mMicroparticle, mWash 1, mLysisDNA,
mMicroparticleDNA, and mWash 1DNA) and the liquid
waste from the m2000sp process. Combining sodium
hypochlorite with these reagents and waste may result
in the formation of toxic gases. If this mixture occurs in
a closed container, pressure may build up in the
container.

Perform the following procedure to empty and clean the liquid


waste container.
1. Open the front-left door of the cabinet.
2. Pull the waste tray forward.
3. Disconnect the two tubing connectors from the top of the
liquid waste container by holding the tubing and pressing
in on the quick-connect fitting.
4. Wrap the tubing with absorbent material.
5. Remove the liquid waste container from the tray.
6. Remove the cap from the container and empty the liquid
waste container.
7. Thoroughly rinse the liquid waste container with water or
a detergent solution.
8. Decontaminate the liquid waste container with a 0.1%
sodium hypochlorite.
9. Rinse the liquid waste container with water a minimum of
five times to ensure that all bleach is removed.
10. Replace the cap and place the container back onto the
waste tray.
11. Reconnect the tubing on the liquid waste container by
pressing the tubing with quick-connect fittings onto the
two connectors on the cap. Ensure the fittings and
connections are secure.
12. Push the waste tray back into the cabinet and close the
front-left door of the cabinet.

m2000sp G-series Service Manual 8-11


200942-103—September 2007
Maintenance Section 8

RoboScrub Procedure
This procedure purges the system liquid pathway, including
tubing, valves, pumps and connectors with a detergent
solution (1.5% solution of RoboScrub). It should be performed
only at the recommendation of Abbott Molecular GSS.
WARNING: Potential Biohazard. Follow basic
biohazard precautions. Appropriate personal protective
equipment should be worn during all cleaning and
assay procedures.

1. Prepare a 1.5% solution of RoboScrub. Add 15 mL of


RoboScrub to 1 L of hot (approximately 50°C) water.
2. Open the front left door of the cabinet. Pull the waste tray
forward.
3. Disconnect the two connectors from the top of the liquid
waste container by holding the tubing and pressing in on
the quick-connect fitting.
4. Empty the liquid waste container. Rinse the liquid waste
container thoroughly with water to remove any sample
preparation reagents or waste containing guanidine
compounds. Replace the cap and tubing.
5. Disconnect the white connector from the top of the
system liquid container by holding the tubing and
pressing in on the quick-connect fitting. Remove the cap
from the container and place in a container filled with the
1.5% solution of RoboScrub. Then reconnect the cap with
the quick-connect fitting.
6. In the m2000sp application, select <System>,
<Maintenance Procedure>, <Extensive Flush>.
7. Wait five minutes, and select <System>, <Maintenance
Procedure>, <Extensive Flush> again.
8. Wait ten minutes, and remove the cap and tubing from
the RoboScrub solution.
9. Disconnect the tubing from the top of the cap, and rinse
the cap and tubing with distilled or deionized water.
10. Blot the tubing dry, and then place the tubing into a
container filled with distilled or deionized water. Connect
the tubing to the top of the cap.

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200942-103—September 2007
Section 8 Clean Liquid System Procedures

11. In the m2000sp application, select <System>,


<Maintenance Procedure>, <Extensive Flush>. Perform
this procedure twice.
12. Disconnect the tubing from the top of the cap, and rinse
the cap and tubing with distilled or deionized water.
13. Blot the tubing dry, and then place the cap and tubing
back on the system liquid container, after first filling the
container with fresh distilled or deionized water.
14. In the m2000sp application, select <System>,
<Maintenance Procedure>, <Extensive Flush>.
15. Remove cap and tubing from the liquid waste container,
and empty the container. Replace.
16. Push the tray back into the cabinet and close the door.
17. In the m2000sp application, select <System>,
<Maintenance Procedure>, <Extensive Flush>.
18. If bubbles still remain in the system, repeat the Extensive
Flush or Simple Flush until all bubbles are removed.

m2000sp G-series Service Manual 8-13


200942-103—September 2007
Maintenance Section 8

Gas Spring Maintenance Check

This annual procedure verifies the integrity of the safety door’s


gas springs. This procedure should not be performed on
springs that have been in place for 5 years or more; springs of
this age must be replaced.
1. Open and close the door several times to ensure that
operation is smooth.
2. Open the door all the way and then lower it until there is
a 10 inch difference between the tip of the top panel and
the top of the door, as shown below.

10 inches

Figure 8.1: Door Lowered to Proper Position

3. Leave the door in this position for five minutes and


observe for slippage.
4. If no slippage occurs, the springs are fine. If slippage
occurs, replace the springs.

8-14 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 Maintenance Schedule

Maintenance Schedule
At Each Site Visit
• Perform Microbial Growth Inspection on page 8–8
• Print system information (refer to Information on page
11–57 of Section 11)
• Run counter readout (refer to the individual device’s
Information page within S&S)

Biannual
Refer to the m2000sp G-series Biannual Preventive
Maintenance Checklist on page 8–19 for a complete listing of
biannual maintenance procedures.
This section discusses special conditions FSEs need to be aware
of and provides a general listing of where specific checklist
procedures or tests can be found in this manual.

Parts to be Replaced
There are two different DiTi Spare Sets available depending on
your instrument’s DiTi O-ring configuration. If you plan to
upgrade to the new DiTi/O-ring configuration, refer to TSB
605-008 for directions. Otherwise, be sure to order the
appropriate part. Refer to DiTi Adapter (DiTi Spare Set)
Replacement on page 9–63 of Section 9 and the part numbers
listed in Section 12.

Parts to be Cleaned
• The following items can be cleaned with alcohol and a
lint-free cloth:
– Z-drive rod and guide rods for RoMa
– Z-drive rod for LiHa
– Arm guide
– Lower DiTi Eject linkage and guide rods
• To clean the Y-square bar, use a lint-free cloth (do not use
any agent). Remove side covers of LiHa as described in
Complete LiHa Assembly on page 9–40 of Section 9.

m2000sp G-series Service Manual 8-15


200942-103—September 2007
Maintenance Section 8

• To clean the system liquid assembly, refer to Clean the


System Liquid Container Procedure on page 8–8.
• To clean the liquid waste assembly, refer to Clean the
Liquid Waste Container on page 8–10.

Lubrication
Part number 10626104 (see Section 12 for details) consists of:
• One tube of food-grade lubricant grease, which is used for:
– the RoMa guide rods
– the Lower DiTi Eject Pivot Pin
• One tube of Lubricant grease, which is used for:
– the LiHa, RoMa, and PosID X-slide shafts
IMPORTANT: Never use a spray-on lubricant.
!
NOTE: Lubricant should be applied sparingly and only
when necessary.

Setup and Service (S&S) Procedures


The S&S procedures referenced on the Biannual Preventive
Maintenance Checklist can be found in Section 4 of this
Manual.

Maintenance and Diagnostic (M&D) Procedures


The M&D procedures referenced on the Biannual Preventive
Maintenance Checklist can be found in Section 5 of this
Manual.

8-16 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 Maintenance Schedule

Annual
Refer to the m2000sp G-series Annual Preventive Maintenance
Checklist on page 8–21 for a complete listing of annual
maintenance procedures.
This section discusses special conditions FSEs need to be aware
of and provides a general listing of where specific checklist
procedures or tests can be found in this manual.

Parts to be Replaced
• DiTi Spare Sets: Be sure to order the appropriate part for
your O-ring configuration. Refer to DiTi Adapter (DiTi
Spare Set) Replacement on page 9–63 of Section 9.
• 3-way Valves: Be sure to order the appropriate valve for
your dilutor configuration.
– For any new XP Smart Dilutors, order valve 10649016
– For any XP3000 Dilutors, order valve 04J97-42

Parts to be Cleaned
• The following items can be cleaned with alcohol and a
lint-free cloth:
– Z-drive rod and guide rods for RoMa
– Z-drive rod for LiHa
– Arm guide
– Lower DiTi Eject linkage and guide rods
• To clean the Y-square bar, use a lint-free cloth (do not use
any agent). Remove side covers of LiHa as described in
Complete LiHa Assembly on page 9–40 of Section 9.
• To clean the system liquid assembly, refer to Clean the
System Liquid Container Procedure on page 8–8.
• To clean the liquid waste assembly, refer to Clean the
Liquid Waste Container on page 8–10.

m2000sp G-series Service Manual 8-17


200942-103—September 2007
Maintenance Section 8

Lubrication
Part number 10626104 (see Section 12 for details) consists of:
• One tube of food-grade lubricant grease, which is used for:
– the RoMa guide rods
– the Lower DiTi Eject Pivot Pin
• One tube of Lubricant grease, which is used for:
– the LiHa, RoMa, and PosID X-slide shafts
IMPORTANT: Never use a spray-on lubricant.
!
NOTE: Lubricant should be applied sparingly and only
when necessary.

Setup and Service (S&S) Procedures


The S&S procedures referenced on the Annual Preventive
Maintenance Checklist can be found in Section 4 of this
Manual.

Maintenance and Diagnostic (M&D) Procedures


The M&D procedures referenced on the Annual Preventive
Maintenance Checklist can be found in Section 5 of this
Manual.

8-18 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 m2000sp G-series Biannual Preventive Maintenance Checklist

m2000sp G-series Biannual Preventive Maintenance Checklist


Customer Information
Contact account: Service Technician:
Contact person: G-series 150
Address: Arm right
RoMa Standard
LiHa 8
ZIP Code: Positive Identification
City: Serial Number:
Country:

Maintenance Procedures
Done/
Action Item N/A Failed Comment
Passed
Diti cones, Tubing
extensions, O-rings
Replace
Any missing or damaged
worktable frogs

Z-drive rod and guide


rods RoMa

Z-drive rod LiHa

Y-square bar LiHa

Arm guide

Liquid waste assembly


Clean (bottle and cap)

System liquid assembly


(bottle and cap)

Lower DiTi Eject linkage


and guide rod

X-slides for PosID, RoMa,


and LiHa

Check and Lower DiTi Eject rocker


Align and stop setting

m2000sp G-series Service Manual 8-19


200942-103—September 2007
Maintenance Section 8

Done/
Action Item N/A Failed Comment
Passed
Lower DiTi Eject pivot pin
Lubricate
RoMa guide rods
(see page
8–16) X-slides for PosID, RoMa,
and LiHa

Tip Adapter Test

Calibrate Lower DiTi Eject

S&S Lower DiTi Eject Test

Liquid Detection Test

Verify sensor calibrations

RoMa 1 mL Carrier Test

RoMa DiTi Rack Test

RoMa DiTi Shelf Test

M&Ds LiHa Sample Tube Check

Z-Calibration Test
(Sample Extraction)

Z-Calibration Test (Master


Mix Addition)

Additional Comments

Signatures

Maintenance Date Service Technician Next Maintenance Date Due

8-20 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 m2000sp G-series Annual Preventive Maintenance Checklist

m2000sp G-series Annual Preventive Maintenance Checklist


Customer Information
Contact account: Service Technician:
Contact person: G-series 150
Address: Arm right
RoMa Standard
LiHa 8
ZIP Code: Positive Identification
City: Serial Number:
Country:

Maintenance Procedures
Done/
Action Item N/A Failed Comment
Passed
Syringes

3-way valves

1:4 manifolds

Pipetting tubing

Interconnecting tubing

FaWa tubing

Replace ILID cables

Clamps

Y-distributors

Syringes

Diti cones, Tubing


extensions, O-rings

Any missing or damaged


worktable grids

m2000sp G-series Service Manual 8-21


200942-103—September 2007
Maintenance Section 8

Done/
Action Item N/A Failed Comment
Passed

Clean/ Door Gas springs


Inspect and
Door Hinges
Replace (if
needed) RoMa Z-brake

Z-drive rod and guide


rods RoMa

Z-drive rod LiHa

Y-square bar LiHa

Arm guide

Liquid waste assembly


Clean (bottle and cap)

System liquid assembly


(bottle and cap)

Lower DiTi Eject linkage


and guide rod

X-slides for PosID, RoMa,


and LiHa

Check and Lower DiTi Eject rocker


Align and stop setting

Lower DiTi Eject pivot pin


Lubricate
RoMa guide rods
(see page
8–18) X-slides for PosID, RoMa,
and LiHa

8-22 m2000sp G-series Service Manual


200942-103—September 2007
Section 8 m2000sp G-series Annual Preventive Maintenance Checklist

Done/
Action Item N/A Failed Comment
Passed
Tip Adapter Test

Fill/Flush FaWa

Calibrate Lower DiTi Eject


S&S
Lower DiTi Eject Test

Liquid Detection Test

Verify sensor calibrations

RoMa 1 mL Carrier Test

RoMa DiTi Rack Test

RoMa DiTi Shelf Test

LiHa Sample Tube Check

Sample Extraction Test


M&Ds
Z-Calibration Test
(Sample Extraction)

Z-Calibration Test (Master


Mix Addition)

Chiller and Heater


Temperature Check

Additional Comments

Signatures

Maintenance Date Service Technician Next Maintenance Date Due

m2000sp G-series Service Manual 8-23


200942-103—September 2007
Maintenance Section 8

8-24 m2000sp G-series Service Manual


200942-103—September 2007
Section 9: Repair, Replacement of Spare Parts

SECTION 9:
REPAIR, REPLACEMENT
OF SPARE PARTS

m2000sp G-series Service Manual 9-1


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

NOTES

9-2 m2000sp G-series Service Manual


200942-103—September 2007
Section 9 Table of Contents

Table of Contents
TOC
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-6
Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Required Special Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Removal of Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . 9-8
Installation of Worktable . . . . . . . . . . . . . . . . . . . . . . . . 9-9
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-10
System Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-11
Spare Parts Discussed in This Section . . . . . . . . . . . . . . 9-11
OPTIBO Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-12
Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-17
CU Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-17
DC-DC Power Board . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-21
SMIO and SMIO-SAFY Board . . . . . . . . . . . . . . . . . . . . 9-24
Power Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-29
Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-29
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-31
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-31
X-Drive Assembly and X-Belt (for LiHa and RoMa) . . . . . . 9-32
Spare Parts X-drive Assembly . . . . . . . . . . . . . . . . . . . . 9-32
X-drive Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-32
X-drive Belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-36
Liquid Handling Arm (LiHa) . . . . . . . . . . . . . . . . . . . . . . . . 9-39
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-39
Complete LiHa Assembly. . . . . . . . . . . . . . . . . . . . . . . . 9-40
Electronic Boards and Cables . . . . . . . . . . . . . . . . . . . . 9-43
Y-belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-52
Y-motors (Y-DC-Servo or Y-spreading). . . . . . . . . . . . . . 9-56
DiTi Mechanism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-63
DiTi Adapter (DiTi Spare Set) Replacement . . . . . . . . . 9-63
White Separator Ring and Black O-ring Assembly . . . . 9-67
Lower DiTi Eject Option . . . . . . . . . . . . . . . . . . . . . . . . 9-68
Tip Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-75
Robotic Manipulator Arm (RoMa Standard) . . . . . . . . . . . 9-77
Special Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-77
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-77
RoMa Standard Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-78
Ensuring Operating Readiness . . . . . . . . . . . . . . . . . . . 9-80
Complete RoMa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-81
Mechanical Adjustment after Reinstallation . . . . . . . . 9-83
Grippers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-89
Gripper Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-92

m2000sp G-series Service Manual 9-3


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Gripper Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-97


Rotator Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-103
Y-flex Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-104
Gripper/Rotator Flex Cables . . . . . . . . . . . . . . . . . . . . 9-107
Y/R- and Z/G-DC-Servo Motor Boards . . . . . . . . . . . . 9-110
RoMa 2 Backplane . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-114
Y-belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-117
Y-motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-121
Z-motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-124
Z-brake . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-128
RoMa Freedom Backplane . . . . . . . . . . . . . . . . . . . . . 9-132
Device CU . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-136
X-DC-Servo Power Board. . . . . . . . . . . . . . . . . . . . . . . 9-139
Ensuring Operating Readiness . . . . . . . . . . . . . . . . . . 9-141
Transport and Packaging . . . . . . . . . . . . . . . . . . . . . . 9-142
PosID 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-143
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-143
Complete PosID 2 Assembly . . . . . . . . . . . . . . . . . . . . 9-144
X-drive Assembly PosID 2 (X-belt, X-motor, X-flex Cable) .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-147
Electronic Boards . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-153
Scanner Assembly (B-motor, Scanner Head, and Scanner
Cable) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-162
Y-drive Assembly (Y-belt, Gripper Assembly, Y-motor) 9-166
No Tube Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-172
Liquid System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-174
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-174
Tubing System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-174
Monitored Pump Option/Fast Wash Option . . . . . . . 9-176
Dilutors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-182
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-182
Dilutor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-182
3-Way Valve and Syringe . . . . . . . . . . . . . . . . . . . . . . 9-184
VCC-Dilback . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-185
Level Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-188
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-188
Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-188
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-190
Sensor Adjustment and Calibration . . . . . . . . . . . . . . 9-190
Door Locks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-191
Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-191
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-192
1 mL Subsystem Assembly . . . . . . . . . . . . . . . . . . . . . . . . 9-193
Complete 1 mL Subsystem Assembly . . . . . . . . . . . . . 9-194
1 mL Subsystem Heater Block . . . . . . . . . . . . . . . . . . . 9-195

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Section 9 Table of Contents

1 mL Subsystem Heater Boards . . . . . . . . . . . . . . . . . . 9-197


1 mL Subsystem Internal Cables . . . . . . . . . . . . . . . . . 9-200
1 mL Subsystem Cables, from OPTIBO Board . . . . . . . 9-200
Chiller/Output Assembly . . . . . . . . . . . . . . . . . . . . . . . . . 9-203
Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-203
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-204
Hood Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-205
CAN-BUS Resistor Test . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-206
Quick Check for Jumper Settings . . . . . . . . . . . . . . . . 9-207
Movement Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-208
Return of Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-209
Service Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-210

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Repair, Replacement of Spare Parts Section 9

Introduction
This section provides instructions on repair and replacement
of spare parts on the m2000sp.
IMPORTANT: Be sure to verify the instrument you are
! servicing is a G-series and not an E-series model. (For
E-series model service, see the m2000sp E-series Service
Manual.)
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.
WARNING: Class 2 Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing of the bar code laser beam or
reflections of the beam.
WARNING: Electrical Shock Hazard. Possible danger
from electrical shock. Switch off the main power and
disconnect the main power connection of the
instrument before replacing fuses, printed circuit
boards, major sub-assemblies, etc. Replace only fuses
that are externally accessible and labeled. Only use
replacement fuses of the specified type and electrical
rating.

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Section 9 Introduction

WARNING: Pinch Point. The instrument requires


accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
CAUTION: Hot Surface.

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Repair, Replacement of Spare Parts Section 9

Worktable
NOTE: Two people will be needed to perform this
installation.
CAUTION: The worktable might have sharp edges.
Take care when handling.

Spare Parts
• Worktable
• Positioning pins
• Sealing strip

Required Special Tool


Reference tip for LiHa (refer to Reference Tips on page 4–54 of
Section 4)

Removal of Worktable
The worktable must be removed if:
• A new or different type of worktable must be installed.
• Access to the PosID 2 is necessary.

Do the following to remove the worktable:


1. Switch off the instrument.
2. Empty the worktable completely, including racks, carriers,
wash/waste station, 1 mL Subsystem, the Output Deck and
door locks (2).
3. Open the front worktable cover. Remove the sealing strip.
4. Remove all six screw caps and all 12 fixing screws (M2.5)
from the worktable.
NOTE: Use a watchmaker’s screwdriver.

5. Remove the deck pins from the rear two rows of grids 1
and 2, in order for the worktable to slide under the PosID.
6. Align the PosID with the opening created on the
worktable.

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200942-103—September 2007
Section 9 Worktable

7. Remove the worktable by carefully lifting and pulling


forward.
• Be careful not to catch the frogs on the PosID. Lift up
on PosID as the worktable is pulled forward.
• The rear lip of the worktable can catch on the
cross-members on the frame. Lift the rear edge of the
worktable up slightly and continue to pull forward.

Figure 9.1: Worktable Removed

Installation of Worktable
1. Carefully install the worktable. If the worktable has deck
pins mounted, remove the rear two rows from grids 1 and
2.
2. Position the PosID on the far right side of the worktable.
3. Insert the oval head screws, and tighten only slightly.
Loosely insert the countersunk screws.
4. Manually align the worktable then further tighten the
front-left screw. This screw acts as a pivot point during the
following alignment.
5. Install the reference tip onto position 3 of the LiHa.
6. Manually position the reference tip at the edge of the
foremost positioning pin in grid position 1 (far left grid).
Check the gap between pin and reference tip.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

7. Move the LiHa to the right-most grid position and check


the gap between pin and reference tip either manually or
using the LiHa hand controls.
NOTE: The gap must be the same on both sides of
the worktable. Adjust the worktable until this is
the case.

Figure 9.2: Gap between Reference Tip and Positioning Pin

8. Tighten all screws, working from left to right and front to


back in a zigzag pattern in order to prevent warping or
bending of the worktable, which will cause PosID
problems.
9. Reinstall all previously removed parts.

Verification
Ensure operating readiness by:
• Checking reference positions (Determine LiHa Reference
Position on page 4–60 of Section 4 and Determine RoMa
Reference Positions on page 4–78)
• Configure the PosID 2 on page 4–87

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Section 9 System Electronics

System Electronics
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Spare Parts Discussed in This Section


• OPTIBO board
• Fuses
• CU board
• DC-DC board
• SMIO/SAFY
NOTE: For all boards, if the m2000sp application is
running while the instrument is powered off and
the boards are switched, a 2501 (FW version
mismatch) error may result when the instrument
is powered on and the firmware on the board does
not match as expected. If the application is
powered off, then a 2513 error with specific board
information will be given.
Perform a firmware download if either of these
firmware errors occur.

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Repair, Replacement of Spare Parts Section 9

OPTIBO Board
The OPTIBO board is located in the left service compartment.
It holds the CU board on its solder side.

OPTIBO

Figure 9.3: OPTIBO Board Location

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Section 9 System Electronics

Removal
Perform either a full system shutdown of the SCC or ensure
that the m2000sp application has been closed by opening
Genesis Setup and Software.
1. Verify the instrument is switched off and the power cord
is disconnected.
2. Disconnect all eight cables from the front of the OPTIBO
board.
NOTE: Remember to disconnect the Dilutor cable
(J4) on the solder side of the PCB.

C
H

G D
F
E

A Host RS-232 E CAN Opt 4-1 mL Sub-


B CAN Opt 1-FaWa system
C CAN Opt 2-CU PosID F Option 6-J18 SMIO
D CAN Opt 3-1 mL Sub- G LiHa J5-LiHa
system H Arm R1 J15 RoMa
Figure 9.4: OPTIBO Board

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Repair, Replacement of Spare Parts Section 9

Figure 9.5: OPTIBO

3. First label then disconnect power supply cables (four


screws) from the OPTIBO.

Figure 9.6: Power Supply Cables

4. Remove the OPTIBO board fixing screws (eight screws: two


along left side, six along right side). Be careful to not
damage the PCB connected to the OPTIBO board’s rear
side.

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Section 9 System Electronics

5. Disconnect the CU board from the OPTIBO board as


described in CU Board on page 9–17.

Figure 9.7: OPTIBO Board

Installation
1. Verify the instrument is switched off and the power cord
is disconnected.
2. Ensure the CU board is connected to the OPTIBO board
solder side.
3. Verify that switch SW1 on the OPTIBO board is set to off
(to the right) for both the System and Option CANs.

Figure 9.8: Switch SW1

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Repair, Replacement of Spare Parts Section 9

4. Connect the Dilutor cable (J4) to the solder side of the


OPTIBO.
NOTE: Refer to the following illustration for
correct cable connection.

Jumper SW1

PC Cable

CAN-BUS Cables for Options

RoMa and LiHa Cables


Dilutor Cable

CAN-BUS Cable for Options


or CANDI, Uniport OPTIBO Board
CU Board

Figure 9.9: OPTIBO Cable Connections

5. Connect the OPTIBO to the instrument with eight screws.


6. Connect all cables to the OPTIBO board.

Verification
Ensure operating readiness by performing the following:
• Firmware test
• Initializing of all system devices
• FaWa Fill/Flush Protocol on page 4–122 of Section 4
• PosID Functionality Test on page 4–87
• Random Move test
• CAN-BUS Resistance Test on page 4–33

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200942-103—September 2007
Section 9 System Electronics

Fuses
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
NOTE: The fuses are located on the OPTIBO board.
Refer to the following illustration for correct allocation.

CU Board 39-01

Option 1:4

Dilutor
RoMa/R1 R2 39-02
LiHa
CANDI and Uniport or Option 5
Arm L1 L2

39-01Fuse 6.3 AT 39-02Fuse 8 AT, sand-filled


Figure 9.10: Fuses on OPTIBO Board

CAUTION: Use sand-filled 8 AT type fuses only—except


the fuse for the CU board, which is 6.3 AT, and not
sand-filled.

CU Board
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.

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Repair, Replacement of Spare Parts Section 9

• Discharge static electricity from your body before


touching the instrument.
NOTE: The CU board is located in the left service
compartment. It can only be accessed by removing the
OPTIBO board because it is connected to the solder side
of the OPTIBO.

Special Tools
IC release tool

Removal
1. Switch off the instrument.
2. Remove the OPTIBO board as described earlier in OPTIBO
Board on page 9–12; refer to the following illustration.

C
H

G D
F
E

A Host RS-232 E CAN Opt 4-1 mL Sub-


B CAN Opt 1-FaWa system
C CAN Opt 2-CU PosID F Option 6-J18 SMIO
D CAN Opt 3-1 mL Sub- G LiHa J5-LiHa
system H Arm R1 J15-RoMa
Figure 9.11: OPTIBO Board

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Section 9 System Electronics

3. Unscrew the four CU board fixing screws. With pliers,


press the barbs on the two board clips then carefully
remove the CU board from the OPTIBO board.

Figure 9.12: CU Board

4. Use the IC release tool to remove the EEPROM (IC6) if it


will be installed on the new board. Refer to Step 9.13 for
details.
NOTE: The IC6 EEPROM from the old board could
be defective or have bent pins.

LEDs
J1

IC6

J22 CU Board

Figure 9.13: CU Board and Cable Connection

NOTE: EEPROM settings can be saved by the


instrument software. Refer to EEPROM Backup on page
4–132 in Section 4, Installation.

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Repair, Replacement of Spare Parts Section 9

Installation
NOTE: Spare CU boards may be delivered with an older
firmware version. If so, firmware upgrade is necessary.
Refer to Firmware Download in Section 11, Appendix
A - Software Reference Material.
1. Determine if jumpers J1 and J22 on the CU board were set
correctly according to configuration of the instrument.

Figure 9.14: CU Board, Jumper J1

2. Install in reverse order.


NOTE: Refer to Figure 9.13: CU Board and Cable
Connection on page 9–19 for correct cable
connections.

Verification
Ensure operating readiness by initializing all system devices
and performing the following in Setup and Service:
• Firmware test
• Perform Liquid Detection on page 4–127
• Perform FaWa Pump Test on page 4–123
• PosID Functionality Test on page 4–87
• Random Move Test on page 11–141

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Section 9 System Electronics

• Check Door Locks on page 4–115


• LiHa DiTi Location Test on page 5–12
• CAN-BUS Resistance Test on page 4–33

Check Error States


Verify the LED status on the CU board according to the
following table.
Table 9.1: Error States

Green LED Red LED Error


blinking off Normal operation
stays on or off stays on or off Fatal hardware error
blinking on Flash not accessible
blinking blinking Power fail circuit failure
blinking fast blinking fast Firmware download required

DC-DC Power Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Verify the instrument is switched off and the power cord
is disconnected.
2. Disconnect the red and black +24VDC power cables from
the DC-DC power supply board.
3. Disconnect the RJ45 cable from the OPTIBO board and
move it to the side.
4. Unscrew the six screws connecting the DC-DC power
supply board to the instrument, and remove the board.

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Repair, Replacement of Spare Parts Section 9

Installation
1. Verify the instrument is switched off and the power cord
is disconnected.
2. If necessary, attach the earth strips to the DC-DC power
supply board using two screws as shown below.

Figure 9.15: New Power Board with Earth Ground Strip

3. Insert the DC-DC Power Supply board carefully as shown


below, and connect the board to the instrument with six
screws. Note that the two bottom center screws fasten the
braided earth-ground cables to the ground strip.

Figure 9.16: New Power Board Installed

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200942-103—September 2007
Section 9 System Electronics

4. Connect the red and black +24VDC power cables as shown


above. The BLACK cable connects the OPTIBO -24VDC
spade to the DC-DC power supply board spade labeled
GND. The RED cable connects the OBTIBO +24VDC spade
to the DC-DC power supply board spade labeled 24V.
5. Reconnect the RJ45 cable to the OBTIBO.
6. Connect the long +12VDC chiller power supply cable to
the output of the DC-DC power supply board as shown
below. The WHITE wire connects to the VOUT+ spade and
the BLACK wire connects to the VOUT- spade.

Figure 9.17: Installing New Power Cable 1

7. Route the cables through the connectors down the side of


the instrument.

Verification
Ensure operating readiness by performing the following:
• Chiller and Heater Temperature Check on page 5–52 of
Section 5

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Repair, Replacement of Spare Parts Section 9

SMIO and SMIO-SAFY Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
The SMIO and SMIO-SAFY boards are identical in design and
layout, but have different firmware and are used to monitor
different functions. When either board is replaced, note the
address and jumper setting of the replaced board. Ensure that
the firmware is at the correct version number for the board
(SMIO or SMIO-SAFY) being replaced.

SMIO Board
The SMIO board is located near the top of the right service
compartment. There are four cables connected:
• J18 System Liquid Capacitance sensor
• J19 Liquid Waste Capacitance sensor
• J21 Solid Waste Ultrasonic sensor
• J2 CAN-BUS cable between the SMIO and SMIO-SAFY
boards

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Section 9 System Electronics

Address Setting: 2

Jumper J9: Inside 2

SMIO SMIO-SAFY
J2 CAN-BUS J2 CAN-BUS to OPTIBO Bd
J9 Jumper J3 CAN-BUS to SMIO Bd
J18 Sys Liq sensor J24/J5Left door lock
J19 Liq Wst sensor J23/J6Right door lock
J21 Sld Wst sensor J9 Jumper
SW1 Address switch SW1 Address switch
Figure 9.18: SMIO/SMIO-SAFY

Removal
1. Switch off the instrument.
2. Unplug the four cables.
3. Remove the four screws (M2.5) securing the SMIO board to
the frame.

Installation
1. Set the address of the new board to 2 and ensure the J9
jumper is on the inside two pins.
2. Install the SMIO board to the frame with four screws
(M2.5).

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

3. Connect the four cables to the SMIO board.

Figure 9.19: SMIO Board

4. Perform the CAN-BUS Resistance test to verify the jumpers


are set correctly.
5. Switch on the power.
6. Check that the firmware version is the correct one for the
SMIO board. Download the correct version if necessary.
IMPORTANT: There are different paths for G-series
! and E-series instruments. For G-series firmware,
follow this file path for download: C:\program
files\Tecan\Genesis Instrument Software.

Verification
Ensure operating readiness by performing the following:
• Firmware test
• Verify the green light on the SMIO board is blinking
• Verify the sensors are functioning

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Section 9 System Electronics

SMIO-SAFY Board
The SMIO-SAFY board is located near the bottom of the right
service compartment. There are four cables connected:
J3 CAN-BUS cable between the SMIO and SMIO-SAFY
boards
J2 CAN-BUS cable to the OPTIBO board
J5/J24 Door lock left
J6/J23 Door lock right

Address Setting: 1 Jumper J9: Outer 2 pins

Removal
1. Switch off the instrument.
2. Disconnect the four cables.
3. Remove the three screws (M2.5) securing the SMIO-SAFY
board to the frame.

Installation
1. Set the address of the new board to 1 and ensure the
jumper is on the outside two pins.
2. Install the SMIO-SAFY board to the frame with three
screws (M2.5).

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

3. Connect the four cables.

Figure 9.20: SMIO-SAFY Board

4. Perform the CAN-BUS Resistance test to verify the jumpers


are set correctly.
5. Switch on the power.

Verification
1. Firmware test.
2. Verify the green light on the SMIO-SAFY board is blinking.
3. Verify the door locks are functioning. Verification is
performed through the S&S software. Refer to Check Door
Locks on page 4–115.

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200942-103—September 2007
Section 9 Power Module

Power Module
NOTE: Refer to Section 3, Technical Data for more
information.
WARNING: Electrical Shock Hazard. Switch off the
power and disconnect the power cord of the
instrument before performing any service. The power
module in its entirety is a spare part. For safety reasons,
no replacement of individual power module
components is allowed. Do not attempt to repair this
part. Faulty wiring could potentially harm the user and
damage the instrument.
NOTE: The removal and installation of the power
module takes approximately two hours.

Removal
1. Verify the power switch is off and the instrument is not
connected to the power outlet.
2. Open the top cover.
3. Remove the four screws (M2.5) securing the cover located
to the right of the diluters.

Top cover

Figure 9.21: Front Cover

4. Remove the two metallic rails that support the tubing


chain (six screws for the backrail, two screws for the
frontrail) to access screws holding the power supply in
place.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

5. Remove the five fixing screws (33-03) (M3) on the bottom


and the five fixing screws (33-01) on top that hold the
power supply in place.

33-01(5x M4 x 10) 2

33-03 33
4x M3 x 10
2-02

2 Top cover 33-01Fixing screws


2-02 Dilutor case covers 33-03Fixing screws
33 Power module

Figure 9.22: Cover and Screw Locations

6. Disconnect the red and black power cables from the


OPTIBO board. Refer to the following illustration for
details.

or

Figure 9.23: Connection to OPTIBO

NOTE: Jumper J11 must be present on the OPTIBO


board
7. Remove the power module by carefully pulling it forward.

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200942-103—September 2007
Section 9 Power Module

Installation
WARNING: Electrical Shock Hazard. Verify the power
switch is off and the instrument is not connected to the
main (i.e., wall) power outlet.
1. Place the new power module in the instrument.
2. Tape the ends of the power cables together to facilitate
leading the power cables through the cable channel.
3. Secure the power module in place with the five bottom
screws (M3).
4. Secure the power module in place with the five top screws
(M3).
5. Connect the power cables to the OPTIBO board.
6. Install the removed cover.
7. Close the top cover.
8. Connect the power cable to the power outlet and switch
on the instrument.

Verification
Ensure operating readiness by performing the following:
• Perform Liquid Detection on page 4–127
• Random Move Test on page 11–141

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Repair, Replacement of Spare Parts Section 9

X-Drive Assembly and X-Belt (for LiHa and RoMa)


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

Spare Parts X-drive Assembly


• X-belt
• X-drive assembly including motor and X-belt (PN 619854)

X-drive Assembly

Removal
1. Switch off the instrument.
2. Remove the X-bay cover, two screws (M2).

Figure 9.24: X-bay Cover

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200942-103—September 2007
Section 9 X-Drive Assembly and X-Belt (for LiHa and RoMa)

Figure 9.25: X-bay Cover, Removed

3. Disconnect the X-motor cable.

Figure 9.26: X-motor Cable Connection

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Repair, Replacement of Spare Parts Section 9

4. Remove the X-drive assembly (5-01) from the X-slide


(5-02; two screws).

Figure 9.27: X-drive Assembly

5-01

2.0 mm 5-02

5-01 X-drive assembly 5-02 X-slide

Figure 9.28: Distance between X-drive Assembly and X-rod

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200942-103—September 2007
Section 9 X-Drive Assembly and X-Belt (for LiHa and RoMa)

Installation
IMPORTANT: Verify you have the correct G-series part
! (PN 619854) before continuing. E-series X-drives will
not work on G-series instruments.
1. Connect the X-drive assembly to the X-slide with two
screws. Use a feeler gauge to ensure the gap between the
X-drive and X-rod is 2.0 mm.

Figure 9.29: X-drive Assembly

NOTE: If no feeler gauge is available, use two 2.0


mm Allen wrenches.
WARNING: Without the 2 mm gap between the
X-drive and X-rod, the assembly will be either too
tight (causing it to bind) or too loose (causing
skipping).
2. Connect the X-motor cable. Refer to Figure 9.26: X-motor
Cable Connection on page 9–33.
3. Check movement by manually moving the X-drive
assembly back and forth and listening for erratic
movement.
4. Install the X-bay cover with two screws (M2).
5. Switch on the instrument.

Verification
Ensure operating readiness by performing the following:
• Perform the Random Move test.
• Check reference points and scale factor.

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Repair, Replacement of Spare Parts Section 9

X-drive Belt

Removal
1. Power off the instrument.
2. Remove the X-drive assembly.

Figure 9.30: X-drive Assembly

3. Remove the two screws (M3) that secure the cover plate.

Figure 9.31: X-drive Assembly Screw

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200942-103—September 2007
Section 9 X-Drive Assembly and X-Belt (for LiHa and RoMa)

4. Remove the cover plate from the assembly.


NOTE: Be careful to not lose the two bearings or
center belt guide pulley.

Figure 9.32: X-drive Cover Plate

5. Slide the X-drive belt off the pulleys.

Figure 9.33: X-drive Belt

Installation
1. Install the X-drive belt onto the pulleys. Ensure the white
guide gear is centered.
2. Attach the cover plate to the X-drive assembly and secure
with two screws.
3. Connect the X-drive assembly to the X-slide.
4. Attach the RoMa or LiHa to the X-drive assembly.
5. Connect any removed cables and secure with clamps.
6. Use a feeler gauge to verify the gap between the X-drive
assembly and X-rod is 2.0 mm.

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Repair, Replacement of Spare Parts Section 9

7. Check movement by manually moving the X-drive


assembly back and forth listening for erratic movement.
8. Install the X-bay cover with two screws.
9. Power on the instrument.

Verification
Ensure operating readiness by performing the following:
1. Random Move Test on page 11–141
2. Check reference points and scale factor.

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Section 9 Liquid Handling Arm (LiHa)

Liquid Handling Arm (LiHa)


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

Spare Parts
• Y-belt
• DC-Servo board
• DC-Servo Power board
• LiHa board backplane board
• ILID board
• ILID chip
• ILID board flat cable
• ILID cable
• Y DC-Servo motor
• Y-spreading DC-Servo motor
• Solenoid
• DiTi spare set
• Tip adapter
• Lower DiTi Eject assembly
• Drive shaft/pinion gear

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Complete LiHa Assembly

Removal
1. Switch off the instrument.
2. Manually move the RoMa all the way to the right side of
the instrument and the LiHa to the middle of the
instrument.
3. Refer to Step 9.34 below to do the following:
• Remove all DiTi adapters (9-03), tubing shelf cover
(9-04), and side covers (9-01).
• Disconnect the LiHa cable (9-05) and X-motor cable
(9-06) from the LiHa backplane board.
• Remove any cable clamps and wraps.

9-06
9-05
9-01

9-04

9-02

9-01 9-03

9-01 Side cover 9-04 Tubing shelf cover


9-02 Tubing shelf 9-05 LiHa cable
9-03 DiTi adapter 9-06 X-motor cable

Figure 9.34: LiHa Assembly

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Section 9 Liquid Handling Arm (LiHa)

4. Refer to Figure 9.35: LiHa Removal of ILID Cables and


Tubing from Z-rod on page 9–41 to do the following:
• Remove the black covers from the TIP-ADAPT boards.
• Disconnect the ILID cables (9-09) from the TIP-ADAPT
board (9-11) and ILID board (9-12).
• Remove the DiTi cones, tip extensions, and stainless
sleeves.
• Remove the support tubings (9-07) with ILID cables
(9-09) and pipetting tubings (9-08) from the Z-rods
(9-10).

9-07

9-08

9-09

9-10

9-09

J2
9-11
J1
J3
J1: tip adapter /ILID
J2: NPS/ILID board
J3: NPS/tip

9-08

9-12 9-09

9-07 Support tubing 9-10 Z-rod


9-08 Pipetting tubing 9-11 TIP-ADAPT board
9-09 ILID cable 9-12 ILID board

Figure 9.35: LiHa Removal of ILID Cables and Tubing from


Z-rod

5. Remove the tubing shelf (four screws); refer to Figure 9.34:


LiHa Assembly on page 9–40.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

6. Remove the screw holding the lower DiTi eject rear


bearing block. Carefully pull the block and shaft out of the
way to access the screw securing the LiHa arm to the
X-slide.
7. Remove the four screws (two M3, two M2.5) holding the
LiHa arm on the X-slide.
8. Carefully lift the LiHa out of the instrument.

Installation
1. Clean the contact surface of the guide rail, guide rollers,
and support roller with a lint-free tissue and ethyl alcohol.
2. Install in reverse order. Before replacing the support
tubing on the Z-rod:
a. Guide each ILID cable through the Z-rods and
connect to each TIP-ADAPT board.
b. Guide the pipetting tubing through the Z-rods and
connect each one to their respective tips.

Verification
Ensure operating readiness by performing the following:
• Flush tips sequentially to verify tubing routing through
Genesis Setup and Service <System>, <Devices>,
<Dilutor>.
• Tip Adapter Test
• Check reference position
• Random Move test
• Liquid Detection test
• Lower DiTi Eject test
• Precision test
• CAN-BUS Resistance Test on page 4–33
• m2000sp calibrations (LiHa)
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26
– LiHa DiTi Location Test on page 5–12
– Sample Extraction Test on page 5–37

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Section 9 Liquid Handling Arm (LiHa)

Electronic Boards and Cables

DC-Servo

D-C Servo Power

Figure 9.36: LiHa DC-Servo and Power PCB

LiHa Backplane Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Figure 9.37: LiHa Backplane PCB

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Repair, Replacement of Spare Parts Section 9

Removal
1. Switch off the instrument.
2. Remove instrument rear panel.
3. Unplug X-drive Power Cable and Lower DiTi Eject power
cable from the backplane board. Remove the boards
housing cover (four screws - M2). Refer to Figure 9.26:
X-motor Cable Connection on page 9–33 for details.
4. Remove the DC-Servo boards and DC-Servo Power board.
Keep the DC-Servo boards in order because each board has
a unique address.
5. Remove the boards housing from the back of the LiHa
(four screws - M2.5).

9-14

9-13
9-17

9-16
9-15

9-13 DC-Servo Power board 9-16 Boards housing


9-14 DC-Servo board 9-17 LiHa backplane
9-15 Boards housing cover

Figure 9.38: LiHa DC-Servo Boards

6. Disconnect the ILID board flat cable and all Y-motor and
Z-motor cables from the LiHa board backplane board and
lower DiTi Eject Assembly cable (J3).

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Section 9 Liquid Handling Arm (LiHa)

7. Remove the six screws (M2.5) holding the LiHa backplane


board onto the bracket. Remove the backplane.

Installation
1. Ensure the bus termination switch is set to on.
2. Attach the LiHa backplane board to the bracket with six
screws (M2.5).
3. Connect the ILID board flat cable and all Y-motor and
Z-motor cables to the backplane board, and the Lower DiTi
Eject Assembly cable to J3.
NOTE: The cable connectors and board
connections are labeled. Be sure to install the
cables in the correct order. Refer to Table 9.2:
Board Positions on page 9–48.
4. Install the boards housing to the back of the LiHa with
four screws (M2.5).
5. Install the DC-Servo boards and DC-Servo Power board in
the correct order.
6. Attach the boards housing cover with four screws (M2).

Verification
Ensure operating readiness by performing the following:
• Check reference position
• Random Move test
• Liquid Detection test
• Lower DiTi Eject test
• Precision test
• CAN-BUS Resistance Test on page 4–33
• m2000sp calibrations (LiHa)
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

DC-Servo Boards, DC-Servo Power Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Switch off the instrument.
2. Remove the LiHa from the instrument, or if access to the
rear of the instrument is available, remove the instrument
back cover. Refer to Complete LiHa Assembly on page
9–40.
3. Refer to Figure 9.38: LiHa DC-Servo Boards on page 9–44:
• Remove the boards housing cover (9-15), (four
screws).
• Remove the appropriate DC-Servo board (9-14) or the
DC-Servo Power board (9-13).

Large, Brown
Resistor

Figure 9.39: LiHa DC-Servo Power PCB

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200942-103—September 2007
Section 9 Liquid Handling Arm (LiHa)

Green Resistor

Figure 9.40: LiHa Servo PCB

NOTE: The DC-Servo Power board is not the same


board as the DC-Servo boards. Therefore, they
cannot be exchanged. With the connector facing
toward you, note the size and color of the resistor
on the left corner of the board. The resistor on the
DC-Servo Power board has a higher resistance and
is brown; the resistor on the DC-Servo boards is
green.

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Repair, Replacement of Spare Parts Section 9

Installation
Install in reverse order.
NOTE: Before installing a new DC-Servo board, use a
2 mm flathead screwdriver to dial the correct address
on the address switch of the replacement DC-Servo
board.
Table 9.2: Board Positions

Boarda Address Connection Motor


DC-Servo (position 1) 4 J4 Y1
J5 Y2
DC-Servo (position 2) 3 J6 Z3
J7 Z6
DC-Servo (position 3) 2 J8 Z7
J9 Z2
DC-Servo (position 4) 1 J10 Z1
J11 Z8
DC-Servo (position 5) 0 J12 Z5
J13 Z4
J3 lower DiTi eject
DC-Servo Power 5 J16 X1
(position 6)

a. Positions are denoted top-down

Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• Check Each of the Tip Adapters on page 4–58
• Determine LiHa Reference Position on page 4–60
• Perform the Lower DiTi Eject Test on page 4–64
• Perform Liquid Detection on page 4–127
• LiHa Calibrations on page 4–134

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Section 9 Liquid Handling Arm (LiHa)

ILID Board
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Switch off the instrument.
2. Remove the tubing shelf cover, four M2 screws, and left
side cover. Refer to Figure 9.34: LiHa Assembly on page
9–40.
3. Disconnect the ILID board flat cable (J9) and all eight ILID
cables from the ILID board. Refer to Figure 9.35: LiHa
Removal of ILID Cables and Tubing from Z-rod on page
9–41.
C

A B

A ILID board switches C ILID cables (8)


B ILID chips (8)
Figure 9.41: ILID Board

4. Remove the ILID board (four M2.5 screws).

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
Install in reverse order.
Be sure to set the ILID board switches (2 switches) SW1 and
SW2 to eight for the number of tips.

Figure 9.42: ILID Board Switches

Verification
Ensure operating readiness by the following: Perform Liquid
Detection on page 4–127.

ILID Board Flat Cable


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Switch off the instrument.
2. Remove the left side cover and the tubing shelf cover (9-01
and 9-04 on Figure 9.34: LiHa Assembly on page 9–40)
(eight screws - M2).

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Section 9 Liquid Handling Arm (LiHa)

3. Disconnect the ILID board flat cable from the ILID board
(9-12) and LiHa backplane board (9-17) Refer to Figure
9.35: LiHa Removal of ILID Cables and Tubing from Z-rod
on page 9–41 and Figure 9.38: LiHa DC-Servo Boards on
page 9–44.

Installation
Install in reverse order.

Verification
Ensure operating readiness by the following: Perform Liquid
Detection on page 4–127.

ILID Cable
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Switch off the instrument.
2. Remove the left side cover (9-01 on Figure 9.34: LiHa
Assembly on page 9–40) (four screws - M2).
3. Refer to Figure 9.35: LiHa Removal of ILID Cables and
Tubing from Z-rod on page 9–41 to do the following:
• Disconnect the ILID cable (9-09) from the TIP-ADAPT
board (9-11) and ILID board (9-12).
• Remove the support tubing (9-07) from the Z-rod
(9-10).
• Remove the ILID cable (9-09) and pipetting tubing
(9-08) from the Z-rod (9-10) and support tubing
(9-07).

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
1. Install in reverse order:
a. Guide the ILID cables through the Z-rods and support
tubing.
b. Guide the aspiration tubes through the Z-rods and
support tubing.
2. Attach the ILID cable to the ILID board (Tip Connector,
NOT Piezo connector) and TIP-ADAPT board (J1).
3. Attach the left side cover to the LiHa with four screws
(M2).

Verification
Ensure operating readiness by the following: Perform Liquid
Detection on page 4–127.

Y-belt

Removal
1. Switch the instrument off.
2. Remove the LiHa from the instrument (refer to Complete
LiHa Assembly on page 9–40).
3. Remove the left side cover and the tubing shelf cover (9-01
and 9-04 on Figure 9.34: LiHa Assembly on page 9–40)
(eight screws - M2).
4. Refer to Step 9.43 below.
• Remove the Y DC-Servo motor (four screws) or
Y-spreading DC-Servo motor (four screws) to slacken
the Y-belt.

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200942-103—September 2007
Section 9 Liquid Handling Arm (LiHa)

• Cut tie wraps if necessary.

9-18 9-19

9-20

9-18 Y-belt 9-20 Y-spreading DC-Servo


9-19 Y DC-Servo motor motor

Figure 9.43: LiHa Y-belt Removal

5. Remove the Y-belt lock from the Y-slide (two screws - M2)
and take the Y-belt out of the LiHa.

9-21

9-22

9-23

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Repair, Replacement of Spare Parts Section 9

9-21 Y-slide 9-23 Y-belt


9-22 Y-belt lock

Figure 9.44: LiHa Y-belt Assembly

6. Open the Y-belt lock and remove the belt.

LiHa Y-belt Locks

Figure 9.45: LiHa Y-belt Locks

Installation
1. Install in reverse order. Belt length is 1290 mm/50.8 in.
2. Insert the Y-belt assembly into the arm belt lock and close.
3. Insert the Y-belt assembly into the LiHa. Do not attach the
Y-belt to the motor pulley at this time.
4. Move the Y-belt and lock into position on the Y-slide.
5. Pull the Y-belt over the Y-motor pulley.
6. Manually tighten the Y-belt by moving the Y-DC-Servo
motor assembly.
7. Tighten the four screws securing the motor assembly.
8. Install the cover.
9. Install the LiHa.

Verification
Ensure operating readiness by performing the following:
• Determine LiHa Range on page 4–60
• Determine LiHa Reference Position on page 4–60
• Random Move Test on page 11–141

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Section 9 Liquid Handling Arm (LiHa)

• Perform Liquid Detection on page 4–127


• CAN-BUS Resistance Test on page 4–33
• LiHa Calibrations on page 4–134
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45

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Repair, Replacement of Spare Parts Section 9

Y-motors (Y-DC-Servo or Y-spreading)


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Remove the LiHa from the instrument. Refer to Complete
LiHa Assembly on page 9–40 for details.

9-19

9-20

9-19 Y DC-Servo motor 9-20 Y-spreading DC-Servo


motor
Figure 9.46: LiHa Y-motors

2. Remove the boards housing (four screws - M2.5) from the


rear of the LiHa. Disconnect the motor cable from the
backplane.
3. Remove the Y-belt as previously described; refer to Y-belt
on page 9–52.
4. Remove the tie wraps to free the cables.
5. Remove the Y-DC-Servo motor or Y-spreading DC-Servo
motor by unscrewing the four screws (2.5 mm Allen key).
6. Remove the pinion gear.

Installation
1. Install in reverse order.
2. Install the pinion gear onto the motor.

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Section 9 Liquid Handling Arm (LiHa)

3. Place the new Y-DC-Servo motor assembly onto the LiHa


and insert the screws. Do not tighten the screws. Ensure
the attachment encoder head points to the correct
direction. Refer to Figure 9.46: LiHa Y-motors on page
9–56.
4. Insert the Y-belt as previously described; refer to Y-belt on
page 9–52.
5. Connect the motor cable to the backplane.
6. Install the boards housing (four screws - M2.5) to the rear
of the LiHa.

Verification
Ensure operating readiness by performing the following:
• LiHa Range calibration reference position calibration
• m2000sp calibrations (LiHa)
• Random Move test
• CAN-BUS Resistance test
• The following M&Ds from Section 5:
– Dispense Test 50 µL, Gravimetric on page 5–23
– Dispense Test 800 µL, Gravimetric on page 5–26
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45

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Repair, Replacement of Spare Parts Section 9

Drive Shaft/Pinion Gear (square or star-shaped)


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
NOTE: If replacement of one drive shaft/pinion gear is
required, replace the other seven at the same time
regardless of condition.

Removal
1. Remove the LiHa from the instrument. Refer to Complete
LiHa Assembly on page 9–40 for details.
2. Remove the screw of the front bushing of the Z-axis drive
shaft/pinion gear to be replaced. See Figure 9.47 below.

Screw

Bushing

Figure 9.47: Front bushings

3. Remove the bushing.

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Section 9 Liquid Handling Arm (LiHa)

4. Loosen the coupling’s set screw and remove the drive


shaft. See Figure 9.48 below.

A Set screw
B Drive shaft
C Retainer screws

Figure 9.48: Drive shaft removal

5. Spread the Z-rods to access the screws of the retainers.


If accessing a screw is difficult, disengage the pin (A
below) of the spreading links from the Z-rod by sliding
the Z-rod down and removing it from the Z-guide. This
will allow the Z-guide to move more freely.

A Pin
B Slot
C Z-guide

Figure 9.49: Spreading link pin

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Repair, Replacement of Spare Parts Section 9

6. Remove the retainer screw and then the retainer.

A Retainer screw
B Retainer
C Z-rod

Figure 9.50: Retainer and Z-rod

7. Remove the tip adapter from the Z-rod:


a. Remove the DiTi.
b. Remove the cover from the TIP-ADAPT board and
disconnect the ILID cable from the TIP-ADAPT board.
c. Remove the set screw holding the tip adapter to the
Z-rod.

Set screw

ILID cable

Figure 9.51: Tip adapter removal

d. Hold on to the Z-rod with one hand and carefully pull


out the tip adapter from the bottom of the Z-rod.
8. Pull the Z-rod out of the Z-guide until the gear is no longer
engaged.

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Section 9 Liquid Handling Arm (LiHa)

9. Extract the pinion gear Z-axis together with the ball


bearings.

Z-rod guide

Pinion gear
Z-axis

Figure 9.52: Pinion gear Z-axis

10. Repeat the above process for the remaining seven drive
shaft/pinion gears.

Installation
Install the drive shaft and pinion gear Z-axis in reverse order of
removal. However, be mindful of the following:
• Do not lubricate the parts.
• Do not apply force when pushing in the Z-rod; turn the
pinion gear Z-axis in such a way that it engages properly
in the Z-rod.
• If the spreading link pin was removed, make sure it is
properly reinstalled in the slot of the Z-rod.

Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• Determine LiHa Range on page 4–60
• Determine LiHa Reference Position on page 4–60
• Calibrate the Lower DiTi Eject on page 4–63
• Perform the Lower DiTi Eject Test on page 4–64
• Y-Spread Procedure on page 4–67

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Repair, Replacement of Spare Parts Section 9

• Perform Liquid Detection on page 4–127


• LiHa Calibrations on page 4–134
• LiHa DiTi Location Test on page 5–12
• Dispense Test 50 µL, Gravimetric on page 5–23
• Dispense Test 800 µL, Gravimetric on page 5–26
• Sample Extraction Test on page 5–37

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Section 9 DiTi Mechanism

DiTi Mechanism
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

DiTi Adapter (DiTi Spare Set) Replacement

(Type B)

Figure 9.53: DiTi Gripping Mechanism Assembly

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Repair, Replacement of Spare Parts Section 9

Please Note
If your instrument was installed prior to
April 2007 and DOES NOT bear the label
shown here, refer to White Separator Ring
and Black O-ring Assembly on page 9–67
before performing the DiTi Adapter
Replacement.

Removal
1. Switch off the instrument and open the front safety panel.
2. Manually move up all Z-racks until they touch lightly,
then move the entire assembly toward the front of the
instrument. Spread the Z-racks completely apart.
3. Loosen the DiTi cone with the supplied wrench. Support
the tip adapter while loosening the cone.
NOTE: If the adapter cylinder within the black tip
ejector tube turns instead of the DiTi cone
(preventing DiTi cone removal), refer to Removal
of DiTi Cone with Loose Adapter Cylinder below.
4. Remove the DiTi cone and ejector tube.
5. Remove the O-ring.
6. Pull out the tubing extension and disconnect the tubing.
7. Unscrew and remove the adapter cylinder.

Removal of DiTi Cone with Loose Adapter Cylinder


If the adapter cylinder turns instead of the DiTi cone, the cone
cannot be removed because the dispense tubing inside of the
adapter cylinder will not rotate freely. Additionally, the
adapter cylinder cannot be tightened in this position because
the tip ejector tube completely covers it. Using a pliers,
perform the following steps to correct this situation.

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Section 9 DiTi Mechanism

1. Place the pliers on the tip ejector tube over the knurled
portion of the adapter cylinder, which will be found just
below the rectangular tip adapter.
2. Gently squeeze the pliers.
NOTE: If the pliers are not placed over the knurled
surface, they will not prevent the cylinder from
spinning regardless of the pressure applied.
3. Place the DiTi cone wrench on the DiTi cone.

Figure 9.54: Pliers and DiTi Wrench Positioned Correctly

4. Rotate DiTi wrench clockwise while holding the pliers in


place. Do NOT turn the pliers.
5. If the DiTi cone does not loosen, increase tension on the
pliers while turning the DiTi wrench.
6. When the DiTi cone is successfully removed, return to
Step 4 of Removal above.

Installation
1. Pull the pipetting tubing approximately 10 cm out of the
tip adapter.
2. Screw the adapter cylinder (knurled part up) onto the tip
adapter. Finger tighten only.

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Repair, Replacement of Spare Parts Section 9

3. Push the conical (blank) part of the tubing extension


6 to 8 mm into the tubing. Support the dispense tubing
while inserting the tubing extension. This will prevent
damage to the dispense tubing caused by exceeding the
tubing bend radius. Insert the tubing into the adapter
cylinder.
NOTE: The pipetting tubing has a bend radius of
approximately 13 mm. Do not exceed the bend
radius of the pipetting tubing during insertion of
the tubing extension.
4. Slide the O-ring (red) onto the tubing extension until it is
touching the barrel.

Figure 9.55: Proper O-ring Positioning

NOTE: No separator ring is used with the red


O-ring. Do NOT install a separator ring.
5. Slide the Ejector tube, grooved-end up, over the adapter
cylinder and secure with the DiTi cone.
6. Tighten carefully with the supplied cone wrench.

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Section 9 DiTi Mechanism

White Separator Ring and Black O-ring Assembly

Separator ring
(white)

O-ring (black)

When you install the new-style red O-ring, DO NOT


reinstall the white separator ring. The red O-ring is designed
to replace both the separator ring and the black O-ring.

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Repair, Replacement of Spare Parts Section 9

Lower DiTi Eject Option

Spare Parts
The following items are available:
• Lower DiTi eject option
• Solenoid

Removal of DiTi Eject Option


1. Remove the front and right LiHa covers.

1 Z-rod #3

2 Fixing screw bearing


block

3 Lower DiTi eject option

4 Fixing screw rocker


assembly

2 5 Solenoid

6 Fixing screw solenoid


19 holder
3
7 Solenoid cable
7
19 Bearing block
6 4

Figure 9.56: Removal of Lower DiTi Eject Option

2. Disconnect the solenoid cable (7) from the socket J3 on


the backplane and free the cable from cable holders and
velcro tape.

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Section 9 DiTi Mechanism

3. Unscrew the fixing screw (4) from the housing of the third
Z-rod (1).
4. Unscrew fixing screws (6) and (2) then remove the
complete lower DiTi eject option assembly.

Installation of DiTi Eject Option


1. Install in reverse order.
NOTE: Verify the solenoid holder screws (6) are
tightened.
2. Perform the Rocker Adjustment Procedure on page 9–71.

Verification
Ensure operating readiness by performing the following:
• Calibrate Lower DiTi eject.
• Perform the Lower DiTi Eject test.
• Perform m2000sp Calibration (LiHa)

Removal of Solenoid
1. Remove the front and right LiHa covers.
2. Disconnect the solenoid cable (7 on Figure 9.56: Removal
of Lower DiTi Eject Option on page 9–68) from the socket
J3 on the backplane and free the cable from cable holders
and velcro tape.

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Repair, Replacement of Spare Parts Section 9

3. Open and remove the link lock (9).

14
13 10
12 11

8 Solenoid 12 Solenoid fixing unit


9 Link lock 13 Solenoid holder
10 Solenoid fork 14 Washer
11 Fixing nut
Figure 9.57: Removal of Solenoid

4. Remove the clamp securing the solenoid cable to the front


face of the LiHa.
5. Remove the two screws securing the solenoid holder to the
LiHa.
6. Remove the solenoid fork (10) and fixing nut (11).
7. Loosen the solenoid fixing nut (12), remove solenoid (8)
from the solenoid holder (13).

Installation of Solenoid
1. Install in reverse order.
NOTE: Verify the solenoid holder screws are
tightened.
2. Connect the solenoid cable to the backplane.
3. Verify that the thin part of the lever is approximately in
the center of the fork and is at a 90-degree angle to the
drive shaft. When pushing the drive shaft in the
Y-direction (to the front and rear), notice that the lever
does not touch the fork on the left or right.
4. Perform the Rocker Adjustment Procedure on page 9–71.

Verification
Ensure operating readiness by performing the following:

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Section 9 DiTi Mechanism

• Calibrate Lower DiTi Eject


• Perform the Lower DiTi Eject test

Rocker Adjustment Procedure


Perform the Rocker Adjustment Procedure:
• after installation of the solenoid or complete Lower DiTi
Eject option replacement
• whenever the rocker touches the Z-rods on the LiHa
• if DiTis are not ejected

Solenoid

Fixing nut

Figure 9.58: Solenoid

1. Manually pull back the solenoid axle to check the distance


between the rocker assembly (15) and Z-rods (1) No part of
the rocker assembly can touch the Z-rods at any time.
NOTE: In the eject position, the overlapping
distance between the rocker nose and tip adapter
must be between 2 to 3 mm.

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Repair, Replacement of Spare Parts Section 9

15

1 Z-rod 15 Rocker assembly

Figure 9.59: Rocker Adjustment

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Section 9 DiTi Mechanism

2. If necessary, adjust the overlap between the rocker nose


and tip adapter by means of the set screw (16) on the
rocker stop.
• Turn the set screw clockwise to decrease the overlap.
• Turn the set screw counterclockwise to increase the
overlap.

18

17

16
16 Rocker assembly

17 Rocker shaft

18 Moving lever guide

Figure 9.60: Rocker Assembly

NOTE: The rocker is adjusted horizontally, not


vertically.
3. Loosen the fixing nut.

Solenoid

Fixing nut

Figure 9.61: Solenoid Assembly

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Repair, Replacement of Spare Parts Section 9

4. Reduce the clearance between rocker shaft (17) and


moving lever guide (18) to an absolute minimum (until
almost touching).
• Turn the solenoid axle clockwise to reduce the
clearing until the rocker moves away from the moving
lever guide.
• Turn only ½ turn counterclockwise.
5. Tighten the fixing nut (11) at the solenoid fork.

Verification
• Verify/adjust the LiHa reference positions
• Perform m2000sp Calibrations (LiHa)
• Calibrate Lower DiTi Eject
• Perform the Lower DiTi Eject test
• The rocker must not touch the Z-rods on the LiHa. If it
does, re-perform the rocker adjustment.

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Section 9 DiTi Mechanism

Tip Adapter
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
NOTE: G-series instruments use Type B tip adapters
only. G-series and E-series tip adapters are not
interchangeable. (E-series models use Type D tip
adapters.)

Removal
1. Remove the DiTi adapter.
2. Remove the cover from the TIP-ADAPT board and
disconnect the ILID cable from the TIP-ADAPT board.
3. Remove the set screw (1.5 M) holding the tip adapter to
the Z-rod.

Figure 9.62: Tip Adapter Removal

4. Hold on to the Z-rod with one hand and carefully pull out
the tip adapter from the bottom of the Z-rod.

Installation
1. Slide the Type B tip adapter up into the bottom of the
Z-rod until the shoulder of the tip adapter shaft (below the
alignment hole) is flush with the base of the Z-rod.
2. Align the screw hole in the tip adapter shaft with the set
screw hole in the Z-rod and secure the tip adapter to the
Z-rod with the set screw.

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Repair, Replacement of Spare Parts Section 9

3. Connect the ILID cable to J1 of the TIP-ADAPT board and


replace the cover.

Type B tip
adapter

Figure 9.63: Tip Adapter Shaft

4. Install the Diti adapter.

Verification
• Perform the M&D Sample Extraction Test on page 5–37.
See Section 5, Maintenance and Diagnostic Procedures,
for more information.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Robotic Manipulator Arm (RoMa Standard)


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

Special Tools
• Mounting bracket
• RoMa reference tip
• Black test plate

Overview
The m2000sp uses the RoMa Standard assembly. The RoMa
Standard and Long assemblies use common parts and either
can be shown for illustration purposes.

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RoMa Standard Parts


14 Roma long
14-02 Centric gripper
14-07 (m2000sp uses eccentric)
14-03 Gripper module*
(include gripper)
14-18 14-04 Gripper board
14-11
14-05 Rotator motor
14-07 Gripper/rotator flex
14-17 cables
14-16 14-14 14-08 Z/G-DC-Servo board
14-15 14-09 Y/R-DC-Servo board
14-09
14-10 RoMa 2 backplane
14-11 Y-belt
14-10 14-12 Z-motor
14-08 14-13 Y-motor
14-14 Z-brake
14-15 Y-flex cable
14-16 RoMa Freedom
14-13
backplane
14-12 14-07
14-17 Device CU
14-05
14-18 X-DC-Servo power
board

14-03 * The actual part “gripper


module” (14-03) includes the
eccentric grippers (14-01), not
eccentric grippers (14-02)
14-02

Figure 9.64: RoMa Long Parts

Eccentric Gripper
The spare part designated “Eccentric Gripper” (14-01) is a set
that includes the following components:
• One long gripper finger (14-21)
• One short gripper finger (14-22)

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Shoulder Flat-head screw


screw
14-21

14-22

Figure 9.65: Eccentric Gripper

RoMa Backplane, Device CU, and X-DC-Servo Board

14-17
14-18

14-16

14-16RoMa Freedom backplane 14-18X-DC-Servo power board


14-17Device CU

Figure 9.66: RoMa Standard, RoMa Long Electronic Boards at


Rear

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Repair, Replacement of Spare Parts Section 9

For the Purpose of this Manual


The designation RoMa stands for RoMa Standard or RoMa
Long.
• The procedures for removing and installing parts of the
RoMa described below apply to both the RoMa Standard
and RoMa Long, unless otherwise noted.
• The illustrations usually show the RoMa Standard.

Ensuring Operating Readiness

Purpose
Most procedures described in this section require the entire
RoMa to be removed before a damaged part can be replaced.
Therefore, adjustments and tests described below must be
performed after reinstallation.

Required Special Tools


• Reference tip
• Test plate

Adjustments, Tests
1. Adjust the RoMa mechanically as described in Mechanical
Adjustment after Reinstallation on page 9–83.
2. Perform the CAN-BUS Resistance test if the RoMa Freedom
backplane, located at the rear, was replaced.
If the RoMa was removed or a new RoMa was installed,
perform Genesis Setup and Service RoMa Calibrations,
and m2000sp calibrations.
3. Perform the Rotator Test in Setup and Service.
4. Perform the following M&D checks from Section 5:
• RoMa DiTi Rack Test on page 5–27
• RoMa DiTi Shelf Test on page 5–29
• RoMa 1 mL Carrier Test on page 5–30

After Finishing
1. Reinstall all covers that were removed.
2. Close service doors and X-bay covers.

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3. Return objects that were removed to the worktable.


4. Start the m2000sp in operating mode.

Complete RoMa

Removal
Perform the following steps to remove the RoMa (14).

14-34
14-27

14-28

14

14-33 14-31
14-32
14-29
14-30
14-01

14 RoMa 14-31X-support
14-27Mounting bracket 14-32X-slide
14-28Guide rollers 14-33X-shaft
14-29Support roller 14-34X-flex cable
14-30Guide rail at front

Figure 9.67: Removing the RoMa

1. Switch off the instrument.


2. Manually move the LiHa to the left side of the instrument.
3. Manually move the RoMa to the middle of the
instrument.
4. Remove the gripper fingers.
5. Unplug the X-flex cable (14-34) and remove it from the
cable holder at the rear of the RoMa. Unplug the X-drive
cable from the backplane board.

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Repair, Replacement of Spare Parts Section 9

Figure 9.68: RoMa X-flex Cable and Clamp

6. Unscrew the X-support (14-31) from the X-slide (14-32)


(four screws).

Figure 9.69: X-support and X-slide

7. Carefully lift the RoMa out of the guide rail (14-30) and off
the X-slide (14-32).

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Installation
1. Clean the contact surface of the guide rail, and the guide
rollers and support roller with a lint-free tissue and a small
amount of ethyl alcohol.
2. Insert the mounting bracket to press the guide rollers
(14-28) apart.

Figure 9.70: RoMa Mounting Bracket

3. Install the RoMa onto the guide rail and onto the X-slide.
4. Insert the four screws, but do not completely tighten.
5. Remove the mounting bracket.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

Mechanical Adjustment after Reinstallation

Purpose
After the RoMa is installed, it must be aligned with the guide
pins on the worktable. The purpose of this alignment is to
ensure the RoMa runs parallel to the guide pins when it moves
in the Y-axis. (rear ↔ front).

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Repair, Replacement of Spare Parts Section 9

Installing the Reference Tip


14-35
14-36

14-37

14-38

14-39

14-40
14-41

14-35Z-rods 14-39Gripper finger support


14-36Z-spindle 14-40Protruding screw
14-37Gripper module head 14-41Reference tip
14-38Screws for positioning gripper
finger supports manually
Figure 9.71: Fixing the Reference Tip to the Gripper Module

1. Move the RoMa gripper module head (14-37) by its Z-rods


(14-35) to above the front center of the worktable.
2. Insert the reference tip (14-41) between the two gripper
finger supports (14-39).
• The protruding screw (14-40) must be on the side of
the screws (14-38).
• Turn one of the screws (14-38) until the reference tip
can be inserted between the gripper finger supports
(14-39).

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Section 9 Robotic Manipulator Arm (RoMa Standard)

3. Fix the reference tip (14-41) to the gripper module head


(14-37) by turning one of the screws (14-38) in the
opposite direction. Ensure the reference tip (14-41) is
firmly held by the gripper finger supports (14-39).

Figure 9.72: RoMa Grip

Alignment in Y-axis
1. Pull the gripper module head (14-37) downward.

14-37

14-41
14-42

Figure 9.73: Positioning the Reference Tip

2. Move the reference tip (14-41) close to the right edge of a


guide pin (14-42) in the third row from the front on the
worktable. Distance from guide pin is approximately
0.2 mm.

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Repair, Replacement of Spare Parts Section 9

3. Firmly hold the X-slide with one hand (item 14-32 in


Figure 9.67: Removing the RoMa on page 9–81). Ensure
the RoMa cannot move in the X-axis during the following
test.
NOTE: The move tool under System Devices >
RoMa can also be used to move the RoMa in the
Y-direction. This ensures there is no movement in
the X-direction.

14-41

14-42

02
Figure 9.74: Checking the Distance Guide Pin/Reference Tip

4. Pull the RoMa gripper module head in the Y-axis (from


rear to front) past three guide pins (14-42) toward the
front of the instrument.
The distance between the guide pins (14-42) and
reference tip (14-41) must not exceed 0.2 mm.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Alignment in Y-axis (Continued)


1. Use the set screws (14-43) and (14-46) to adjust the
moving direction of the RoMa.

14-45 14-48

14-47

14-46
14-43
14-44

14-43Left set screw M4 x 10 14-46Right set screw M4 x 10


14-44Left fixing screw M4 x 12 14-47Right fixing screw M4 x 12
14-45Left fixing screw M3 x 10 14-48Right fixing screw M3 x 10

Figure 9.75: Fixing and Adjusting Screws on X-support

2. If the RoMa drifts to the right, turn the right set screw
(14-43) to correct.
3. If the RoMa drifts to the left, turn the left set screw (14-46)
to correct.
4. Repeat steps 1 through 3 until the RoMa Standard moves
parallel to the guide pins.

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Repair, Replacement of Spare Parts Section 9

5. Tighten the fixing screws when finished; refer to Figure


9.71: Fixing the Reference Tip to the Gripper Module on
page 9–84.

0.2 mm

0.5 mm

0.2 mm

0 mm

Figure 9.76: Correction of Drifts

6. Remove the reference pin (14-41).


7. Install the grippers.
8. Connect the X-flex cable (14-34) and X-drive cable to the
RoMa backplane board.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Grippers

Removal
The RoMa gripper set includes:
• 2 gripper fingers of different lengths
– Short gripper finger: 131 mm
– Long gripper finger: 161 mm (has oblong hole
4.2 mm for height adjustment)
• 2 screws
– 1 shoulder-head screw for the short finger
– 1 flat-head screw for the long finger
• 2 pieces of 3M foam tape (for the reference plate)

Flat-head screw

14-38 14-21

Shoulder screw
14-22

14-21Long gripper finger 14-38Screws for moving gripper fin-


14-22Short gripper finger gers manually

Figure 9.77: Gripper Fingers

Removal
Loosen the screws and unscrew the long (14-21) and short
(14-22) gripper fingers.
NOTE: Ensure the shoulder screw stays with the short
finger and the flat-head stays with the long finger.

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Repair, Replacement of Spare Parts Section 9

Installation
1. Install the short gripper finger (14-22) and tighten the
shoulder screw. Ensure it is installed on the correct side as
illustrated in Figure 9.77 above.
2. Position the long gripper finger (14-21) and fasten it
slightly using the flat-head screw.

Verification
• Perform all required RoMa S&S calibrations per this
manual and the latest version of ISA 605-002, including:
– Determine RoMa Reference Positions on page 4–78 of
Section 4
– Determine Range on page 4–81
– Adjust the Rotator on page 4–82
– Manual RoMa Rotator Adjustments on page 4–83
• Perform all RoMa m2000sp calibrations per ISA 605-002;
refer to RoMa Calibrations on page 4–157
• Perform the following M&Ds:
– RoMa DiTi Rack Test on page 5–27 of Section 5
– RoMa DiTi Shelf Test on page 5–29
– RoMa 1 mL Carrier Test on page 5–30

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Adjust Gripper Fingers


1. Check the parallelism of the arms by placing the reference
plate on the deck.
Use the RoMa move tool to position the RoMa arms
directly over the plate, with the grippers open to the
width of the plate.

Figure 9.78: RoMa Positioned Over the Plate

2. If the distance of each gripper finger from the worktable is


not equal, adjust the height:
• Eccentric grippers: Long gripper finger
• Tolerance limit: 0.5 to 1 mm
3. Tighten the fixing screw of the respective gripper finger
when finished.
4. Perform the RoMa Reference Plate test.

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Gripper Board

Overview

14-56
14-57

14-58

14-06
14-54
14-55
14-67 14-04

14-59 14-62
14-60 14-66
14-63
14-0514-69
14-64
14-65
14-68
14-03 14-53
14-22
14-52
14-37 14-21

14-03Gripper module (include grip- 14-55Gripper/rotator flex cables


pers!) clamp
14-04Gripper board 14-56Gripper/rotator flex cables
14-05Rotator motor protection bracket
14-06Gripper/rotator flex cables 14-57Fixing screw (cable protec-
14-21Long gripper finger tion) M3 x 5
14-22Short gripper finger 14-58Gripper/rotator flex cable pro-
14-37Gripper module head tection foil
14-52Rotator cover 14-59 Gripper motor cable
14-53Fixing screws M2.5 x 6 14-60Cable connector
14-54Gripper/rotator flex cables 14-62Rotator motor cable
holder bracket 14-63Cap nut
14-64O-ring
14-65
Figure 9.79: Overview of Rotator and Gripper Modules

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Remove Gripper Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Unplug Cables
1. Unscrew the three screws (1.5M) that secure the rotator
cover (14-52).
NOTE: Refer to Figure 9.79: Overview of Rotator
and Gripper Modules on page 9–92 for details.

Figure 9.80: RoMa Rotator Cover

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Repair, Replacement of Spare Parts Section 9

2. Remove the gripper/rotator flex cables clamps (14-55) by


removing two screws (1.5M).

A Flex cable C Holder bracket


B Clamp
Figure 9.81: RoMa Flex Cable and Clamp

3. Unscrew the two screws (2.5M) that secure the


gripper/rotator flex cables holder bracket (14-54).
4. Disconnect the gripper/rotator flex cables from the gripper
board (14-04).
• for RoMa Long: (14-07)

Figure 9.82: RoMa Rotator and Gripper Flex Cables

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Section 9 Robotic Manipulator Arm (RoMa Standard)

5. Disconnect the rotor motor cable (14-62).

Figure 9.83: RoMa Rotator Gripper Motor Cables

6. Disconnect the gripper motor cable (14-59).

Extract Wires
Use the crimp release tool to extract individual wires from the
connector (14-60). Be careful to not damage the contact
bushes (14-61). Refer to Figure 9.79: Overview of Rotator and
Gripper Modules on page 9–92 for details.

14-60

 14-61

" !
$ #
& %
Figure 9.84: Gripper Motor Cable Plug

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Repair, Replacement of Spare Parts Section 9

Remove Gripper Board


NOTE: Refer to Figure 9.79: Overview of Rotator and
Gripper Modules on page 9–92 for details
1. Remove the cap nut (14-63), O-ring (14-64), and bushing
(14-65).

Figure 9.85: RoMa Gripper Cap Nut

2. Unscrew and remove the gripper PCB (14-04).

Figure 9.86: Gripper Board

Install Gripper Board


1. Install by performing steps above in reverse order.
2. Reinsert the wires into the connector.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

• Make sure to insert the contact bushes (14-61)


correctly into the housing of the connector (14-60).
• Note the wire colors.
Pin 1 White Pin 2 Not used
Pin 3 Yellow Pin 4 Red
Pin 5 Green Pin 6 Black
Pin 7 Not used Pin 8 Violet

14-60

14-61

" !
$ #
& %
Figure 9.87: Wire Colors

3. Continue installation by performing steps 1 through 6 of


Unplug Cables on page 9–93, in reverse order.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

Gripper Module

Removal
1. Remove the grippers.
2. Remove the gripper board.

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3. With one hand, hold the gripper module. Remove the two
gripper module screws (2.5M) using the other hand.

Figure 9.88: Gripper Module

Figure 9.89: Gripper Module Fixing Screws

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Section 9 Robotic Manipulator Arm (RoMa Standard)

4. Carefully remove the gripper module without damaging


the gripper module cable or losing the O-ring in the
gripper flange.

Figure 9.90: Gripper Module Showing Gripper Cable

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Repair, Replacement of Spare Parts Section 9

5. With one hand, hold the gripper module. Lead the gripper
cable through the Z-module using the other hand.

Figure 9.91: Gripper Module

Figure 9.92: Gripper Nut Cable Guide

NOTE: Ensure the gripper positioning screws are


within the range of the stop screw mounted in the
motor plate. The gripper module must be able to
move within 280 degrees.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Figure 9.93: Gripper Module Positioning Screws

Figure 9.94: Flange and Positioning Pins

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Repair, Replacement of Spare Parts Section 9

6. While still holding the gripper module, insert and tighten


the two gripper module screws (2.5M).

Figure 9.95: Gripper Module Fix Screw

7. Install the gripper board.

Figure 9.96: Gripper Board

8. Install the grippers.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Rotator Motor
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
Refer to Figure 9.79: Overview of Rotator and Gripper Modules
on page 9–92 for details.
1. Remove RoMa and gripper board as previously described
(see pages 9–81 and 9–92).
2. Loosen the gripper module head screws (14-68) to access
the fixing screws (14-69) of the rotator motor (14-05).
Refer to Gripper Board on page 9–92.
3. Unscrew and remove the rotator motor (14-05).

Screws

Figure 9.97: Rotator Motor Screws

Installation
1. Reinstall the rotator motor (14-05).
2. Tighten the gripper module head screws (14-68).
3. Reinstall the gripper board and RoMa.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

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Repair, Replacement of Spare Parts Section 9

Y-flex Cable

Removal

14-16

14-15

14-10
14-10 RoMa 2 backplane
14-15 Y-flex cable
14-16 RoMa Freedom backplane

Figure 9.98: Gripper Motor Cable Plug

1. Remove the left cover of the RoMa (nine screws).


2. Remove one cable clamp located on the inside of the cover
(two screws - M2).
3. Disconnect the Y-flex cable (14-15) from the RoMa 2
backplane (14-10) and RoMa Freedom backplane (14-16).

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

A
B

C
D

A Y-flex cable C Z/G-DC-Servo PCB (top)


B Gripper/rotator flex cables D Y/R-DC-Servo PCB (bottom)
E RoMa 2 backplane
Figure 9.99: Y-flex Cable Connected to RoMa 2 Backplane

C
D

A Y-flex cable C Device CU


B RoMa Freedom backplane D X-DC-Servo Power

Figure 9.100: Y-flex Cable Connected to RoMa Freedom


Backplane

m2000sp G-series Service Manual 9-105


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
1. Install in reverse order.
NOTE: Pleat the new flex cable in the same
manner as the old one.

Figure 9.101:

2. Connect the Y-flex cable to the RoMa 2 backplane and


RoMa Freedom backplane.
3. Install the cable clamp on the inside of the cable with two
screws (M2).

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

Gripper/Rotator Flex Cables

General
The gripper/rotator flex cables are two parallel cables—for the
rotator motor and gripper motor—which are connected to the
RoMa backplane and gripper board.

Removal

14-56
14-57
14-08
14-09

14-04 Gripper board


14-06 Gripper/rotator flex cables 14-10
14-08 Z/G-DC-Servo board 14-06
14-09 Y/R-DC- Servo board 14-55
14-10 RoMa 2 backplane 14-54
14-54 G/R flex cables holder bracket 14-04
14-55 G/R flex cables clamps
14-56 Cable protection bracket
14-57 Fixing screw (cable protection)
M3 x 5

Figure 9.102: Gripper Motor Cable Plug

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

1. Unscrew the three screws (M1.5) that secure the rotator


cover.

Figure 9.103:Rotator Cover

2. Remove the gripper/rotator flex cable clamp by removing


two screws (M1.5).

Figure 9.104:RoMa Flex Cable and Clamp

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Section 9 Robotic Manipulator Arm (RoMa Standard)

3. Unscrew the two screws (M2.5) that secure the


gripper/rotator flex cable holder bracket.

Figure 9.105:Flex Cable Holder Bracket

4. Unscrew six screws and remove the cable protection


bracket (14-56).
5. Remove the Z/G- and Y/R-DC-Servo boards (14-08) and
(14-09).

Need to fix pic

A
C
B

A Z/G-DC-Servo PCB (top) B Y/R-DC-Servo PCB (bot-


tom)

Figure 9.106:DC-Servo Boards

6. Disconnect the gripper/rotator flex cables from the RoMa


2 backplane (14-10) and Gripper board (14-04):
• RoMa Long (14-07)

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
Install in reverse order.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

Y/R- and Z/G-DC-Servo Motor Boards


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
Refer to Figure 9.79: Overview of Rotator and Gripper Modules
on page 9–92 for items not illustrated below.

14-09 14-70

14-08

14-71

14-70

14-08Z/G-DC-Servo board 14-70Connectors gripper/rotator


14-09Y/R-DC-Servo board flex cables
14-71Board retaining clamp
Figure 9.107: Servo Motor Boards

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

Removing Boards
1. Remove the left cover (nine screws) and disconnect the
Y-flex cable.

Figure 9.108:DC-Servo Boards

2. Disconnect the gripper/rotator flex cables from the


connectors (14-70) on the RoMa 2 backplane board:
• (14-07) for RoMa Long.
3. Open the board retaining clamp (white-headed push pin)
(14-71).

Need to fix pic

Figure 9.109:RoMa DC-Servo PCB

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

4. Carefully remove the Z/G-DC-Servo board (14-08).


CAUTION: Be careful not to damage the
gripper/rotator flex cables when the board is removed.
Leave the Teflon protection foil (14-58 on Figure 9.79:
Overview of Rotator and Gripper Modules on page
9–92) between the flex cable closest to the shafts and
inner Y/R-DC-Servo board until it is removed.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
5. Open the board retaining clamp (white-headed push pin)
(14-71).

A
A

A RoMa 2 backplane B Teflon protection foil

Figure 9.110:RoMa 2 Backplane Teflon

6. Carefully remove the DC-Servo motor board (14-09) from


the Y-motor.

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

Installing the Boards


1. Verify the address switches are set correctly:
• Switch for Y/R-DC-Servo board (14-09, inner board) is
address 6
• Switch for Z/G-DC-Servo board (14-08, outer board) is
address 7

! " #

$
% & ')
.  
! " # * + ,-

$
% & ')
.  
* + ,-

! " #

$
% & ')
.  
* + ,-

Figure 9.111: Addresses on DC-Servo Motor Boards

2. Install in reverse order.


NOTE: Be careful when installing the
Y/R-DC-Servo board. The fit is very tight. Ensure
the pins are aligned with the connector on the
backplane. Remember to depress the board
retaining clamp (white-headed push pin) when
sliding in the board.
NOTE: The Y/R-DC-Servo board is installed solder-
side up.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

m2000sp G-series Service Manual 9-113


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

RoMa 2 Backplane
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Remove the left cover (nine screws).
2. Disconnect six cables from the RoMa 2 backplane (14-10).

14-09
14-10
14-73

14-08

14-70

14-72
14-73 14-74

14-08Z/G-DC-Servo board 14-72Connector for Y-flex cable


14-09/Y/R-DC-Servo board 14-73Fixing screws (backplane)
14-10RoMa 2 backplane 14-74Spacer
14-70Connectors Gripper/rotator
flex cables

Figure 9.112: Servo Motor Board

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

3. Remove the Z/G- and Y/R-DC-Servo boards (14-08) and


(14-09) according to Y/R- and Z/G-DC-Servo Motor Boards
on page 9–110.

RoMa 2
backplane

Z/G-DC-Servo
board

Figure 9.113:RoMa 2 Backplane and Z/G-DC-Servo Board

4. Unscrew two screws (2.5M) and remove the RoMa 2


backplane (14-10).

Figure 9.114:RoMa 2 Backplane

Figure 9.115:Y/R-DC-Servo Board and Roma 2 Backplane

m2000sp G-series Service Manual 9-115


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Figure 9.116:Teflon Sheet

Installation
Perform steps above in reverse order:
• Install the RoMa 2 backplane.
• Install the Z/G- and Y/R-DC-Servo boards.
• Connect six cables to the Roma 2 backplane.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

Y-belt

Removal
1. Remove the RoMa. Refer to Complete RoMa on page 9–81.

14-77
14-75 14-80
14-11

14-79
14-78

14-11 Y-belt 14-78 Belt lock


14-75Y-pulley (M1.5) 14-79 Belt fixing screw (M2)
14-77Y-belt lock fixing screw 14-80 Belt tensioning screw (not
(M1.5 x 6) directly visible) M2 x 12
Figure 9.117:Y-belt (Front)

2. Loosen the Y-belt lock fixing screw (1.5M) (14-77).

Figure 9.118:Belt Fix Screw

m2000sp G-series Service Manual 9-117


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

3. Remove the belt tensioning screw (M2) (14-80) and belt


lock (14-78).

Figure 9.119:Belt Tensioning Screw (14-80)

4. Loosen the belt fixing screws (M2) (14-79) and remove the
front end of the Y-belt.

Figure 9.120:Belt Fixing Screw (14-79)

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

5. Loosen the screws (M2) (14-82) at the rear until the rear
end of the Y-belt (14-11) can be removed.

Figure 9.121:End Fixing Screws

6. Pull out the Y-belt (14-11).


14-81
14-82

14-11

14-11 Y-belt 14-82 Fixing screw M3 x 8


14-81 Fixing plate
Figure 9.122:Y-Belt

m2000sp G-series Service Manual 9-119


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation

14-11 14-75 14-77 14-80


14-82

14-81

14-79

14-78 14-11

14-11 Y-belt 14-79Belt fixing screw (M2)


14-75Y-pulley 14-80Belt tensioning screw (not
14-77Y-belt lock fixing screw (M1.5 directly visible) (M2 x 12)
x 6) 14-81Fixing plate
14-78Belt lock 14-82Fixing screw (M2 x 8)
Figure 9.123: Fixing the Y-belt at the Front and Rear

1. Lead the Y-belt around the Y-belt pulley.


2. Route the Y-belt through the RoMa.
3. Fix the Y-belt (14-11) at the rear as illustrated on the right
side of Step 9.123 above.
4. Fix the Y-belt (14-11) at the front as illustrated on the left
side of Step 9.123 above.

Figure 9.124:RoMa Y-belt Motor

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

5. Tension the Y-belt by turning the screw (14-80).


6. Secure with the Y-belt lock-fixing screw (14-77) when
finished.
7. Manually move the module back and forth to verify the
Y-belt is assembled correctly.
8. Install the RoMa into the instrument.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

Y-motor

Removal
14-77 14-80
14-11 14-83 14-75

14-10
14-87
14-12
14-90
14-88 14-79
14-78

14-89 14-89

14-10RoMa 2 backplane 14-79Belt fixing screw (M2)


14-11 Y-belt 14-80Belt tensioning screw (not
14-12Y-motor directly visible) (M2 x 12)
14-75Y-pulley 14-84Mounting plate
14-76Pulley fixing screw (M1.5) 14-87Y-motor fixing screws M2 x 6
14-77Y-belt lock fixing screw (M1.5 14-88Cable tie
x 6) 14-89Y-motor cable
14-78Belt lock 14-90Z-motor cable
Figure 9.125: Y-motor

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

1. Remove the RoMa.

Figure 9.126:RoMa Y-belt Lock Fix Screw

2. Cut the cable tie (14-88) and disconnect the motor cables
(14-89) and (14-90) from the RoMa 2 backplane (14-10).
3. Loosen screws (14-77) and (14-80) until the screws (14-79)
become accessible. This releases the belt tension.

Figure 9.127:RoMa Y-belt Tension Screw

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Section 9 Robotic Manipulator Arm (RoMa Standard)

4. Unscrew the mounting plate (14-83), loosen the pulley


fixing screw (M1.5), and remove the Y-pulley (14-75).

Figure 9.128:RoMa Y-belt Motor

5. Remove the screws (M2) and remove the Y-motor.

Installation
1. Insert the Y-motor. Ensure the encoder head points in the
correct direction.
2. Secure the motor to the RoMa with three fixing screws
(M2).
3. Place the Y-belt pulley over the motor axle. Ensure the flat
sector of the Y-motor axle is perpendicular to the pulley
screw.
4. Install the support plate.
5. Secure and tension the Y-belt.
6. Connect the motor cables to the RoMa 2 backplane.
7. Install the left cover with seven screws.
8. Install the RoMa into the instrument.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

m2000sp G-series Service Manual 9-123


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Z-motor
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
14-09

14-08

14-93 14-92

14-91

14-96 14-10
14-94
14-95
14-13

14-08 Z/G-DC-Servo board 14-92Z-motor support


14-09Y/R-DC-Servo board 14-93Positioning hole
14-10RoMa 2 backplane 14-94Z-motor pinion
14-13Z-motor 14-95Fixing screws (M2.5)
14-91Fixing screws M2 x 6 14-96Mounting holes

Figure 9.129: Z-motor

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

1. Remove the RoMa.

Figure 9.130:RoMa 2 Backplane

2. Disconnect all cables from the RoMa 2 backplane (Z- and


Y-motor and Z-break cables, Y-flex, gripper/rotator flex
cables).
3. Remove the Z/G- and Y/R-DC-Servo boards (14-08) and
(14-09) according to Y/R- and Z/G-DC-Servo Motor Boards
on page 9–110.

Figure 9.131:Z/G-DC-Servo

Figure 9.132:Y/R DC-Servo

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

4. Remove the RoMa 2 backplane according to RoMa 2


Backplane on page 9–114.

Figure 9.133:RoMa 2 Backplane

Figure 9.134:Teflon Sheet

5. Unscrew three screws and remove the Z-motor assembly.

Figure 9.135:Y- and Z-motors

6. Separate the Z-motor from the Z-motor support by


removing three screws.

Installation
1. Place the replacement Z-motor on the support. Ensure the
motor encoder head points in the correct direction.
2. Insert and tighten the three motor fixing screws.

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

3. Place the Z-motor assembly on the Z-drive.


4. Ensure there is approximately 0.1 mm of play between the
pinion and Z-drive gear to prevent damage to the Z-motor
pinion and avoid noise.
5. Tighten the screws.
6. Install the RoMa 2 backplane.
7. Install the Z/G- and Y/R-DC-Servo boards onto the RoMa 2
backplane, ensuring they are installed in the correct order
and orientation.
8. Connect the cables to the RoMa 2 backplane.
9. Install the cover.
10. Install the RoMa into the instrument.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

m2000sp G-series Service Manual 9-127


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Z-brake
The old brake assembly featuring the shoe with the curved face
is being phased out. Upon failure, it should be replaced with
BRAKE SOLENOID Z-AXIS COMPLETE ROMA-3 (part number
30027047).

Z-brake Removal

14-100
14-56
14-14

14-98
14-97
14-10
14-14

14-99

14-10 RoMa 2 backplane 14-97Fixing screw


14-14 Z-brake 14-98Z-brake cable
14-56Gripper/rotator flex cables 14-99Brake shoe
protection 14-100 Strain relief
Figure 9.136: Z-brake

1. Disconnect the Z-brake cable (14-98) from the RoMa 2


backplane (14-10). The cable is held by the strain relief
(14-100) near the gripper/rotator flex cables protection
plate (14-56).

A
B
C

A Z-motor cable B Y-motor cable


C Z-brake
Figure 9.137:RoMa 2 Backplane

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

2. Unscrew two screws (M2) and remove the Z-brake (14-14).

Z Brake

Figure 9.138:RoMa Z brake

Z-brake Installation
NOTE: The brake shoe is screwed to the axle and fixed
with the set screw.
NOTE: For replacement of the brake shoe only, refer to
Brake Shoe Replacement on page 9–130 below.
1. Press the brake shoe down to the stop as shown below.

A Brake shoe
B Set screw
C Axle

d = Distance

Figure 9.139:Checking/adjusting the distance

2. Measure the distance between the brake shoe and the


body. The distance must be 7.25 + 0.2 mm (0.285 + 0.008
in).
3. If necessary, adjust the distance as follows:
a. Loosen the set screw.
b. Hold the axle with a pair of pliers and turn the brake
shoe until the distance is correct
c. Carefully tighten the set screw and secure it with
chemical thread lock when the adjustment is done.
4. Install the new Z-brake assembly in reverse order of
Z-brake Removal.

m2000sp G-series Service Manual 9-129


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

• Ensure the parts of the Z-brake cable covered with


shrinkable tubing are fixed securely with the cable
clamps, as shown in Figure 9.140 below.

Figure 9.140:Fixation of Z-brake Cable

Verification
Refer to Ensuring Operating Readiness on page 9–141.

Brake Shoe Replacement


IMPORTANT: This procedure can only be performed on
! Z-brake assemblies that already have the flat-faced
brake shoe. Assemblies with the curve-faced brake shoe
must be replaced with part number 30027047.
Subsequent repairs can be carried out using the
procedure below.
1. Access the Z-brake as described above in Z-brake Removal
on page 9–128.
2. Loosen and remove the set screw.

A Brake shoe
B Set screw
C Axle

d = Distance

Figure 9.141:Z-brake assembly

3. Remove the old shoe and spring.

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

4. Install the new spring and shoe, and secure with the set
screw.
5. Press the shoe down to the stop and measure the distance
between the brake shoe and the body. The distance must
be 7.25 + 0.2 mm (0.285 + 0.008 in).
6. Adjust as needed by loosening the set screw and turning
the brake shoe to achieve the correct distance.
7. Re-install the assembly as described in Z-brake Installation
on page 9–129.

m2000sp G-series Service Manual 9-131


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

RoMa Freedom Backplane


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Overview

F A

D
E
B

A RoMa Freedom backplane D Board holding pin


B Device CU E End belt fixing screws
C DC-Servo Power F X-flex cable connector
G Y-flex cable connector

Figure 9.142: RoMa Freedom Backplane, CU, and Servo Power


Boards

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Removal
1. Remove the RoMa or, if access allows, remove the back
cover.

Figure 9.143:RoMa Freedom Backplane Device CU, X-DC


Servo Power Boards and Cables

14-104
14-17
14-18

14-103

14-16
14-105

14-16RoMa Freedom backplane 14-103Connector for Y-flex cable


14-17Device CU 14-104Connector for X-flex cable
14-18X-DC-Servo Power board 14-105X-motor cable (not directly visi-
bl )
Figure 9.144: RoMa Freedom Backplane, Device CU, and
X-DC-Servo Power Board

m2000sp G-series Service Manual 9-133


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

2. Disconnect all cables from the RoMa Freedom backplane


(14-16):
• Y-flex cable (connector 14-103) J2
• X-flex cable (connector 14-104) J1
• X-motor cable (14-105) J10
3. Depress the two board retaining clamps (white-headed
push pins). Remove the Device CU (14-17) and
X-DC-Servo Power board (14-18).

Figure 9.145:RoMa Freedom Backplane Device CU and X-DC


Servo Power Boards

4. Unscrew and remove the RoMa Freedom backplane (seven


M2.5 screws).

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

Installation
1. Set the address to 4 and verify jumper J2 is terminated.

Figure 9.146:RoMa Freedom Backplane Address

2. Install the RoMa Freedom backplane with seven screws


(M2.5)
3. Install the Device CU and X-DC-Servo Power boards.
Secure with board retaining clamps.
4. Reconnect the Y-flex, X-flex, and X-motor cables.
5. Install the RoMa or back cover, whichever was removed.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

m2000sp G-series Service Manual 9-135


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Device CU
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal

14-104

14-17
14-18

14-16
14-106

14-103
14-105

14-16 RoMa Freedom backplane 14-103 Y-flex cable connector


14-17 Device CU 14-104 X-flex cable connector
14-18 X-DC-Servo power board 14-105 X-motor cable
14-106Address switch for Device CU
Figure 9.147: RoMa Freedom Backplane, Device CU, and More

1. Perform EEPROM backup with the Setup & Service


software.
2. Remove the RoMa, or if access is allowed, remove the back
cover.
3. Open the board retaining clamp (white-headed push pin).

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

Figure 9.148:RoMa Freedom Backplane Device CU, and


X-DC Servo Power Boards

4. Remove the board (14-17).

Figure 9.149:RoMa Device CU Board

Installation
1. The address switch (14-106) for the Device CU board
(14-17) is located on the RoMa Freedom backplane. Set the
address switch on the RoMa to position 4.

Figure 9.150:Address Switch for Device CU Board

2. Install the board onto the RoMa Freedom backplane and


secure with the board retaining clamp (white-headed push
pin).

m2000sp G-series Service Manual 9-137


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

3. Install the RoMa or instrument cover, whichever was


removed.
4. Restore the EEPROM.
NOTE: If bad settings were backed up, bad settings will
be restored.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.
NOTE: If the Device CU board is exchanged without
restoring the EEPROM, the following setups must be
performed:
• Set X-drive properties
• Set machine size
• RoMa setup

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200942-103—September 2007
Section 9 Robotic Manipulator Arm (RoMa Standard)

X-DC-Servo Power Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Remove the RoMa, or if access is allowed remove the back
cover.
2. Remove the lower board retaining clamp (white-headed
push pin) (14-71).

14-104

14-17
14-18

14-16
14-106

14-103
14-105

14-16 RoMa Freedom backplane 14-103 Y-flex cable connector


14-17 Device CU 14-104 X-flex cable connector
14-18 X-DC-Servo power board 14-105 X-motor cable
14-106Address switch for Device CU
Figure 9.151: RoMa Freedom Backplane Device CU and X-DC
Servo Power Boards

m2000sp G-series Service Manual 9-139


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

3. Remove the X-DC-Servo Power board (14-18).

Figure 9.152:RoMa X-DC Servo Power Board Address

Installation
1. The address switch (14-106) for the X-DC-Servo Power
board (14-18) is located on the board itself. Set the address
switch to position 0 (zero).

! " #
$
% & ')
.  

* + ,-

Figure 9.153:Address of X-DC-Servo Power Board

2. Install the X-DC-Servo Power board onto the RoMa


Freedom backplane and secure with the board retaining
clamp.
3. Install the RoMa or instrument cover, whichever was
removed.

Verification
Refer to Ensuring Operating Readiness on page 9–141 for
details.

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Section 9 Robotic Manipulator Arm (RoMa Standard)

Ensuring Operating Readiness

Purpose
Most procedures described in this section require the entire
RoMa to be removed before a damaged part can be replaced.
Therefore, adjustments and tests described below must be
performed after reinstallation.

Required Special Tools


• Reference tip
• Test plate

Adjustments, Tests
1. Adjust the RoMa mechanically as described in Mechanical
Adjustment after Reinstallation on page 9–83.
2. Perform the CAN-BUS Resistance Test on page 4–33 if the
RoMa Freedom backplane, located at the rear, was
replaced.
If the RoMa was removed or a new RoMa was installed,
perform Genesis Setup and Service RoMa Calibrations,
and m2000sp RoMa Calibrations on page 4–157.
3. Adjust the Rotator on page 4–82.
4. Perform the following M&D checks:
• RoMa DiTi Rack Test on page 5–27
• RoMa 1 mL Carrier Test on page 5–30
See Section 5, Maintenance and Diagnostic Procedures,
for more information.

After Finishing
1. Reinstall all covers that were removed.
2. Close service doors and X-bay covers.
3. Return objects that were removed to the worktable.
4. Start the m2000sp in operating mode.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Transport and Packaging


Store and/or ship the RoMa to Abbott Molecular only in its
original transport box using the original foam inserts.
NOTE: The RoMa transport box can also be used to
transport the LiHa arm.
1. Insert the transport padding into the clean, empty box.
2. Remove the two gripper fingers with a 3 mm Allen key.
Refer to Figure 9.65: Eccentric Gripper on page 9–79 for
details.
3. Carefully place the RoMa or LiHa in the transport box as
shown below.

Figure 9.154:Transport Box (LiHa Arm and RoMa)

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Section 9 PosID 2

PosID 2
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
WARNING: Class II Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing of the bar code laser beam or
reflections of the beam.

Spare Parts
• PosID 2 assembly
• X-belt
• X-flex cable
• X-DC-Servo motor
• PosID 2 CU board
• X-DC-Servo board
• Y/B board
• Y/B-DC-Servo board
• DSP decoding board
• B-DC-Servo motor
• Scanner head

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

• Scanner cable
• Y-belt
• Gripper assembly
• Y-DC-Servo motor
• No Tube sensor

Complete PosID 2 Assembly

Removal
1. Switch off the instrument.
2. Remove the left and right door locks.
3. Remove the worktable; see Worktable on page 9–8.
4. Disconnect the PosID 2 connection cable (13-03) and
X-motor cable (13-02) from the PosID 2 CU board.

13-01

13-02
13-04

13-03

13-01Communication cable 13-03Connection cable


13-02X-motor cable 13-04PosID 2 CU board

Figure 9.155:PosID 2 Cable Connections

5. Remove the PosID 2 assembly fixing nuts (four nuts).

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Section 9 PosID 2

6. Slide the PosID 2 module (13) to the left side.


7. Hold the assembly by the base plate (13-08), lift the right
side first, then carefully lift the complete PosID 2 assembly
out of the instrument.

13

13-10

13-09

13-08

13-07
13-06

13-02
13-03

13-05

13 PosID 2 module 13-07PosID board


13-02X-motor cable 13-08Base plate
13-03Connection cable 13-09X-flex cable
13-05X-motor 13-10X-belt
13-06X-shaft
Figure 9.156:PosID 2 Assembly

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
1. Install in reverse order.

13-08

13

13 PosID 2 module 13-08Base plate

Figure 9.157:Installation of PosID 2 Assembly

2. For PosID modules manufactured after May 2002, move


the base plate (13-08) to the far left of the instrument.
Before screwing it into the instrument frame, ensure there
is exactly 2 mm of space behind the base plate.
3. Before installing the worktable:
• Check for smooth and even motion of the PosID 2
module (13)
• Verify correct movement of the X-flex cable (13-09)
• Ensure the X-shaft (13-06) and base plate (13-08) are
thoroughly clean

Verification
Ensure operating readiness by performing the following:
• Worktable Alignment

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Section 9 PosID 2

• Configure the PosID 2 on page 4–87


• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
• CAN-BUS Resistance Test on page 4–33

X-drive Assembly PosID 2 (X-belt, X-motor, X-flex Cable)


NOTE: For the X-DC-Servo board, refer to Electronic
Boards on page 9–153 for details.

X-belt

Removal
1. Switch off the instrument.
2. Remove the worktable; see Worktable on page 9–8.
3. Loosen the belt tensioning screws (13-13) (two screws).

Figure 9.158:Belt Tensioning Screws

4. Unscrew X-belt fixture to access the X-belt fixing screw


(13-14). The X-belt is secured to the PosID module under
the module slide.
5. Remove the X-belt.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
1. For a new belt, cut the X-belt to the required length: 2430
mm.
2. Run the X-belt over the idler pulley (13-12) and drive
pulley of the X-motor; insert belt ends into the fixture and
tighten the fixing screw (13-14).

13-12

13-13
2x M4x8

13-05

13-14 M4x20

13-05X-motor 13-13Tensioning screws


13-12Idler pulley 13-14X-belt fixing screws

Figure 9.159:X-drive Assembly, Details

3. Fasten the X-belt fixture to the X-slide.

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200942-103—September 2007
Section 9 PosID 2

4. Adjust and tighten the tensioning screws (13-13). The belt


must be tight and horizontal, but not over-tightened.

Figure 9.160:Adjust Tensioning Screws

Verification
Ensure operating readiness by performing the following:
• Align the worktable as described in Installation of
Worktable on page 9–9
• Configure the PosID 2 on page 4–87
• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
– CAN-BUS Resistance Test on page 4–33

X-motor

Removal
1. Switch off the instrument.
2. Remove the worktable; see Worktable on page 9–8.
3. Disconnect the X-motor cable (13-02) from the PosID
board (13-07). Refer to Figure 9.156: PosID 2 Assembly on
page 9–145 for details.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

4. Loosen the belt tensioning screws (13-13).


5. Remove pulley (mL.5 screw) from the X-motor assembly
then unscrew the X-motor (13-05) from the bracket (four
screws). Refer to Figure 9.159: X-drive Assembly, Details
on page 9–148.

Installation
Install in reverse order.
• Adjust the distance between the drive pulley and bracket
to approximately 5.5 mm.

Verification
Ensure operating readiness by performing the following:
• Worktable alignment (see Installation of Worktable on
page 9–9)
• Configure the PosID 2 on page 4–87
• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
• CAN-BUS Resistance Test on page 4–33

X-flex Cable

Removal
1. Switch off the instrument.
2. Remove the worktable; see Worktable on page 9–8.
3. Remove casing as illustrated in Figure 9.165: Y/B Board
Assembly on page 9–155.
4. Disconnect the X-flex cable from PosID board. Refer to
Figure 9.156: PosID 2 Assembly on page 9–145.

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Section 9 PosID 2

4x M3x6
2x M3x8

Figure 9.161:Flex Cable Fixtures

5. Remove the two flex cable fixtures.


6. Unscrew the five Y/B board fixing screws.

13-20
5x M2x6

Figure 9.162:Y/B Board Fixing Screws

7. Disconnect the X-flex cable from the Y/B board.

Installation
1. Fold the X-flex cable on the end where it will be connected
to the PosID.

185 mm (7¼”)

185 mm (7¼”)
Figure 9.163:X-flex Cable

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

2. Install in reverse order.


3. Before reinstalling the worktable, verify correct movement
of the X-flex cable, and adjust if necessary.

Verification
Ensure operating readiness by performing the following:
• Worktable alignment (see Installation of Worktable on
page 9–9)
• Configure the PosID 2 on page 4–87
• M&D checks from Section 5:
– LiHa Sample Tube Check on page 5–14
– Sample Extraction Test on page 5–37
– Z-Calibration Check – Sample Extraction on page
5–40
– Z-Calibration Check – Master Mix on page 5–45
• CAN-BUS Resistance Test on page 4–33

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Section 9 PosID 2

Electronic Boards
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

PosID 2 CU Board, X-DC-Servo Board


The PosID CU board is located at the bottom of the left service
compartment. The X-DC-Servo board is connected to the
PosID 2 CU board.

Removal
1. Switch off the instrument.
2. Disconnect all cables from the PosID 2 CU board.
3. Unscrew and remove X-DC-Servo board (two screws).

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

4. Unscrew the PosID 2 CU board. (five screws).

J8

J6

Figure 9.164:PosID 2 CU Board

Installation
1. Verify jumper J6 (for L-CAN Bus) is set (jumper closed).
2. Verify jumper J8 is set according to the communication
diagram (jumper open).
3. Verify the X-DC-Servo board address switch is set to #0.
4. Install in reverse order of removal.

Verification
Ensure operating readiness:
• CAN-BUS Resistance Test on page 4–33
• Configure the PosID 2 on page 4–87

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200942-103—September 2007
Section 9 PosID 2

Y/B Board, Y/B-DC-Servo Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal

13-17
13-16 13-18

13-15

3x M2x6

13-15casing 13-17Y/B board


13-16Y/B-DC-Servo board 13-18DSP decoding board

Figure 9.165: Y/B Board Assembly

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

1. Remove the three screws (M2) which secure the casing


(13-15).

Figure 9.166:PosID

2. Disconnect all cables (Y-motor, B-motor, X-flex, scanner,


No Tube sensor cables) from the Y/B board (13-17).

Figure 9.167:Y/B Board

3. On the exterior of the PosID 2, unscrew five fixing screws


(M2) (13-20) and remove the Y/B board.

13-20
5x M2x6

Figure 9.168:Y/B Board Fixing Screws (13-20)

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Section 9 PosID 2

4. Push in the white pin on the bracket and remove the


Y/B-DC-Servo board (13-16).

Figure 9.169:Y/B-DC-Servo Board

5. Disconnect two cables (J7 and J9 from the DSP decoding


board) (13-18).

Figure 9.170:DSP Decoding Board (13-18)

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

6. Remove three screws securing the DSP decoding board to


the Y/B board (M2.5).

Figure 9.171:Y/B Board (M2.5)

Figure 9.172:Y/B PosID 2

Installation
1. On the Y/B-DC-Servo board, verify the address switch is
set to #2.
2. Attach the DSP decoding board to the Y/B board with
three screws.
3. Connect two cables from the DSP decoding board to the
Y/B board.
4. Install the Y/B-DC-Servo board to the Y/B board. Secure
with the white-headed push pin.

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200942-103—September 2007
Section 9 PosID 2

5. Secure the Y/B board to the PosID assembly with five


screws.
6. Connect the Y-motor, B-motor, No Tube sensor, X-flex,
and scanner cables to the Y/B board.

Scanner Cable
X-flex Cable

Y-motor Cable

Connections to DSP
Board

B-motor Cable
No Tube Sensor Cable

Figure 9.173:Y/B Board Cable Connections

Verification
Ensure operating readiness:
• Configure the PosID 2 on page 4–87

DSP Decoding Board


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Removal
NOTE: The referenced position numbers are illustrated
in Figure 9.164 on page 9-144.
1. Remove the three screws that secure the casing (13-15).

Figure 9.174:PosID

2. Disconnect the two cables (J7 and 9) from DSP decoding


board (13-18) and three screws that secure the DSP
decoding board to the Y/B board.

Figure 9.175:Y/B Board

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200942-103—September 2007
Section 9 PosID 2

3. Remove the DSP decoding board.

Figure 9.176:Y/B PosID 2

Installation
1. Attach the DSP decoding board to the Y/B board with
three screws.
2. Connect two cables (J7 and J9) from the DSP decoding
board to the Y/B board.
3. Attach the casing with three screws.

Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Scanner Assembly (B-motor, Scanner Head, and Scanner Cable)

B-motor

Removal
1. Remove the three screws that secure the casing (13-15) as
illustrated in Figure 9.165: Y/B Board Assembly on page
9–155.
2. On the exterior of the PosID 2, unscrew the five Y/B board
fixing screws (13-20) to free the B-motor cable (13-24).
Disconnect this cable from Y/B board (J4).

13-19
2x M4x8

13-20
13-17 5x M3x6

13-24
13-23
13-22
M5x6

4x M2.5x8 13-21

13-17Y/B board 13-22Set screw


13-19B-motor fixing screws 13-23Bracket
13-20Y/B board fixing screws 13-24B-motor cable
13-21B-motor

Figure 9.177: B-motor

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Section 9 PosID 2

3. On the exterior of the PosID 2, unscrew two fixing screws


(13-19) for removal of the B-motor assembly.
4. Loosen the set screw (13-22) and remove the gearing
assembly from the motor.Exterior of PosID 2
5. Remove four screws securing the bracket (13-23) to the
B-motor (13-21).

Figure 9.178:B-motor Bracket

Installation
1. Install in reverse order.
NOTE: Observe correct positioning of the encoder
head as illustrated in Figure 9.177: B-motor on
page 9–162.
2. Use four screws to attach the B-motor to the bracket.
3. Tighten the set screw on the motor gearing assembly.
4. Secure the B-motor assembly to the PosID assembly with
two exterior screws.
5. Connect the B-motor cable to the Y/B board at J4.
6. Secure the Y/B board to the PosID assembly with five
screws.
7. Attach the casing with three screws.

Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Scanner Head, Scanner Cable

Removal
1. Remove the three screws securing the casing (13-15) as
illustrated in Figure 9.165: Y/B Board Assembly on page
9–155.

13-25

13-28
13-29
13-26 13-27 Potential
sticking point

13-25Cable connector 13-28Housing


13-26Scanner head 13-29Scanner cable
13-27Fixing screws

Figure 9.179:Scanner Head Assembly

2. Remove the scanner head housing (13-28) by removing


two screws.

Figure 9.180:Scanner Head Cover

3. Unscrew the cable holders and cable connector (13-25),


then disconnect the scanner cable (13-29) from the Y/B
board.

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200942-103—September 2007
Section 9 PosID 2

• Open the fixing screws (13-27) and remove the


scanner head (13-26).

Figure 9.181:Scanner Head Assembly

Installation
1. Install in reverse order.
NOTE: Swivel the scanner head up and down and
arrange the cable (13-29) so it does not touch and
stick at the point indicated with an arrow in Figure
9.179: Scanner Head Assembly on page 9–164.
2. Attach the scanner head with two screws.
3. Connect the scanner cable to the Y/B board.
4. Connect the cable holders and cable connector.
5. Attach the scanner head housing with two screws.
6. Attach the casing with three screws.

Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Y-drive Assembly (Y-belt, Gripper Assembly, Y-motor)

Y-belt, Gripper Assembly

Removal
1. Remove three screws (M2) securing the casing (13-15)
Figure 9.165: Y/B Board Assembly on page 9–155.
2. Loosen the two belt tensioning screws (M2.5) (13-36 in
Step 9.183 below).

Figure 9.182:PosID Belt Tension Screws

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Section 9 PosID 2

13-30

13-31

13-32
4x M3x6

13-33

13-34

13-35
2x M2x6 13-36

13-30Y-motor cable 13-34Gripper assembly


13-31Y-motor 13-35No tube sensor
13-32No Tube sensor cable 13-36Belt tensioning screws
13-33Y-belt
Figure 9.183: Y-drive Assembly

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

3. Remove the four screws (M2) securing the gripper


assembly (13-34) and remove the Y-belt (13-33).

A Gripper assembly screws B Driver pins (reverse side)

Figure 9.184:Gripper Assembly

Installation
1. Lead the Y-belt around the driver pins (bottom side of
gripper assembly) as shown below.

Figure 9.185:Y-belt and Driver Pins

2. Install the Y-belt.


3. Attach the gripper assembly with four screws.
4. Manually tension the belt and tighten the two belt
tensioning screws.
5. Ensure smooth operation of the belt.
6. Attach the casing with three screws.

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Section 9 PosID 2

Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87

Y-motor

Removal
1. Remove casing (13-15) as illustrated in Figure 9.165: Y/B
Board Assembly on page 9–155.
2. Loosen the two belt tensioning screws (M2.5) (13-36).
Refer to Figure 9.183: Y-drive Assembly on page 9–167 for
details.

Figure 9.186:PosID Belt Tension Screws

3. Disconnect the Y-motor cable (13-30) from Y/B board.

Figure 9.187:Y/B Board

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

4. On the exterior of the PosID 2, unscrew the two fixing


screws (M2) and remove the Y-motor assembly.

2x M3x8

Figure 9.188:Y-motor Fixing Screws

5. Remove drive pulley (set screw) and screws securing the


bracket to the Y-motor.

Figure 9.189:Y-motor

Installation
Install in reverse order.
• Note the correct orientation of the encoder head. Refer to
Figure 9.183: Y-drive Assembly on page 9–167 for details.
• When installing the drive pulley, ensure the set screw is
perpendicular to the flat part of the axle.
• Tension the Y-belt as described earlier in Y-belt, Gripper
Assembly on page 9–166, for details.

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Section 9 PosID 2

Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

No Tube Sensor

Removal
NOTE: The referenced parts are illustrated in Figure
9.183: Y-drive Assembly on page 9–167.
1. Switch off the instrument.
2. Remove the casing (13-15) as illustrated in Figure 9.165:
Y/B Board Assembly on page 9–155.
3. Unscrew the cable clamps (M2.5) then disconnect the No
Tube sensor cable (13-32) from the Y/B board.
4. Remove the two screws (M1.5) used to secure the No Tube
sensor (13-35).

A No tube sensor B Gripper

Figure 9.190:No Tube Sensor

Installation
Install in reverse order.
1. Place the No Tube sensor into the PosID assembly and
secure with two screws.
2. Route the No Tube sensor cable to the Y/B board and
connect the cable to the board.
3. Secure the cable in place with the cable clamps.
4. Install the casing.

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Section 9 PosID 2

Verification
Ensure operating readiness by performing the following:
• Configure the PosID 2 on page 4–87

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Liquid System
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. The exposure to hazardous chemicals is
minimized by following instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.

Overview
The term “liquid system” refers to all instrument modules and
parts which contain or directly influence liquid. The main
components include:
• LiHa Freedom; refer to Liquid Handling Arm (LiHa) on
page 9–39
• Tips; refer to the m2000sp Operations Manual
• Tubing system; refer to Tubing System below.
• MPO/FWO; refer to Monitored Pump Option/Fast Wash
Option on page 9–176
• Dilutor/Dilback; refer to Dilutors on page 9–182

Tubing System
This section describes the standard tubing system. Refer to
Monitored Pump Option/Fast Wash Option later in this
section for MPO/FWO tubing system details.

Spare Parts
Tubing and fittings for standard tubing

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Section 9 Liquid System

Standard Tubing
8 Tips
30-03

30-02
8
30-01
4
30-06 32 9
30-07
30-04
35

30-05

30-01Aspiration tubing 30-07Distributor 1:4


30-02Interconnecting tubing 4 Dilutor
30-03Pipetting tubing 8 DiTi assembly
30-04Wash station 9 DiTi adapter
30-05Waste tube 32 Dilback
30-06Y-connector 35 MPO/FaWa
Figure 9.191: Tubing

Replacement
1. Empty the liquid system.
2. Switch off the instrument.
3. Replace relevant tubing.
NOTE: When replacing the pipetting tubing,
remember that diluter #1 (on the far left) is
connected to tip #1 (closest to the rear of the
instrument), diluter #2 to tip #2, etc.

Verification
Ensure operating readiness by performing the following:
• Tightness check
• Perform FaWa Pump Test on page 4–123
• The following M&D checks from Section 5:

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

– Dispense Test 50 µL, Visual on page 5–21


– Dispense Test 800 µL, Visual on page 5–24
– Sample Extraction Test on page 5–37

Monitored Pump Option/Fast Wash Option

General

Fast Wash Option (FWO) Fast Wash pump without LICOS


liquid level sensors

Spare Parts MPO/FWO


• FWO complete
• Fast Wash (FaWa) pump
• Pressure relief valve
• Distributor 1:2
• Tubing
• Connector cable

MPO/FWO Complete

Removal
1. Empty the liquid system.
2. Switch off the instrument.
3. Remove the two outer screws of the left worktable cover
(M2.5 x 8).

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200942-103—September 2007
Section 9 Liquid System

4. Pull the Fast Wash pump assembly out of the instrument


like a drawer.

2 screws M2.5 x 8
Figure 9.192:Fast Wash Pump Assembly Removal

5. Disconnect the tubing from the Fast Wash pump, pressure


relief valve, and connection cable from the OPTIBO.

Installation
Install in reverse order.
• Electronic connections: OPTIBO, Option CAN-MPO
board, J7
• Connect the tubing as shown in Step 9.193 below

35-1

35-2 35-3 35-4

35-8
35-7
35-6
35-5

MPO/FWO Standard

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Repair, Replacement of Spare Parts Section 9

35-1 Aspiration tube (to diluter) 35-5 Aspiration tube to FaWa


35-2 Distributor 1:2 pump
35-3 Pressure tube 35-6 Waste tube for pressure relief
35-4 FaWa pump valve
35-7 Pressure relief valve

Figure 9.193: MPO/FWO Tubing Connections

Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123

Fast Wash Pump

Removal
1. Remove the Fast Wash pump assembly from the
instrument.
2. Remove the tubing from the FaWa pump.
3. Remove the FaWa pump from the Fast Wash pump
assembly (five screws).

FaWa Pump Fixing Screws

Figure 9.194:View from the Backside of the MPO/FWO


Assembly Drawer

Installation
1. Install in reverse order. Connect the tubing as shown in
Figure 9.193: MPO/FWO Tubing Connections on page
9–178
2. Use the Dilutor panel of the LiHa to fill the Fast Wash
pump. A dry pump valve may not work properly.

Verification
Ensure operating readiness by performing the following:

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200942-103—September 2007
Section 9 Liquid System

• Check all tubing fittings for tightness


• Perform FaWa Pump Test on page 4–123

Pressure Relief Valve

Removal
1. Remove the Fast Wash pump assembly from the
instrument.
2. Remove the tubing from the pressure relief valve.
3. Remove the pressure relief valve from the Fast Wash pump
assembly (1 screw).
NOTE: Be careful not to confuse the attachment
screw of the pressure relief valve with the
regulating screw. Do not alter the setting of the
regulating screw.

Figure 9.195:Pressure Relief Valve

Installation
Install in reverse order. Ensure the arrows on the valve match
the direction of fluid flow as shown above in Figure 9.195.

Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

MPO/FWO Tubing

Removal
1. Remove the Fast Wash pump assembly from the
instrument.
2. Remove the tubing.

Installation
Install in reverse order. Refer to Figure 9.193: MPO/FWO
Tubing Connections on page 9–178 for correct tubing
connections.

Verification
Ensure operating readiness:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123

MPO Board
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Remove the Fast Wash pump assembly from the
instrument. The MPO board is located at the backside of
the assembly.
2. Disconnect the FaWa pump cable and connection cable.
3. Remove the MPO board (5 screws).

Figure 9.196:MPO Board Fixing Screws

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200942-103—September 2007
Section 9 Liquid System

Installation
Install in reverse order.
• Connection cable: J7
• FaWa pump cable
Go into the <Liquid System>, <FaWa>, <Setup> menu, and
set the number of pumps to 1 and number of tips for pump 1
to 8.

Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Dilutors
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Spare Parts
• Dilutor type XP 3000 plus
• 3-way valve, 120°, M6 fittings
• Syringe
• VCC-Dilback

Dilutor

Removal
1. Empty the liquid system using the Dilutor Panel of the
LiHa.
2. Switch off the instrument.
3. Move the LiHa and RoMa arms to one side.
4. Open top cover (2 screws).
5. Remove diluter case cover (2 screws).

2-01

2-02

2-01 Top cover 2-02 Dilutor case cover

Figure 9.197:Dilutor Case

6. Remove the interconnecting and pipetting tubing from


the diluter.

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200942-103—September 2007
Section 9 Dilutors

7. Unscrew the diluter from the instrument (1 M3 x 12 screw


with washer underneath the diluter).
NOTE: Be careful to not mistake the steel sheet
screws for diluter screws.
8. Carefully pull the diluter out of the instrument.

Installation
1. Verify the CAN-BUS address is set correctly.

Figure 9.198:Dilutor Address Settings

2. Install in reverse order.


Address settings:
• Dilutor 1: 0
• Dilutor 2: 1
• Dilutor 3: 2
• Dilutor 4: 3
• Dilutor 5: 4
• Dilutor 6: 5
• Dilutor 7: 6
• Dilutor 8: 7
Dilutor 1 is the left-most diluter.
3. Perform a visual check to verify the liquid system is tight
(no leaking, dripping, etc.).

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
• Perform Liquid Detection on page 4–127
• Perform the following M&D checks:
– Dispense Test 50 µL, Visual on page 5–21
– Dispense Test 800 µL, Visual on page 5–24
See Section 5, Maintenance and Diagnostic Procedures
for more information.

3-Way Valve and Syringe

Removal
1. Unscrew the knurled plunger lock screw that secures the
syringe to the plunger drive block.

Valve connection

3-way valve

Syringe screw

Plunger lock screw

Figure 9.199:Syringe Assembly

2. Unscrew the syringe screw and remove the syringe.

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200942-103—September 2007
Section 9 Dilutors

3. Unscrew the input tubing (from 1:4 distributor) and


output tubing (to the DiTi) from the 3-way valve.

Output to DiTi Input from distributor

M3 x 25

Figure 9.200:Tubing Removal

4. Remove the two phillips head screws (M3 x 25) that secure
the valve to the diluter and remove the valve.

Installation
1. Secure the 3-way valve to the diluter using two phillips
head screws.
2. Connect the input tubing (from the 1:4 distributor) and
the output tubing (to the DiTi) to the 3-way valve.
3. Insert the syringe and tighten the syringe screw.
4. Tighten the knurled plunger lock screw to secure the
syringe to the plunger drive block.

Verification
Ensure operating readiness by performing the following:
• Check all tubing fittings for tightness
• Perform FaWa Pump Test on page 4–123
• The following M&D checks from Section 5:
– Dispense Test 50 µL, Visual on page 5–21
– Dispense Test 800 µL, Visual on page 5–24

VCC-Dilback
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

• Discharge static electricity from your body before


touching the instrument.

Removal
NOTE: The VCC-Dilback is positioned in the diluter
case behind the diluters.

Figure 9.201:VCC-Dilback

1. Verify the instrument is switched off and the power


connection is disconnected.
2. Remove all diluters from the instrument.
3. Disconnect the cable leading to OPTIBO Freedom (J1).

Figure 9.202:VCC-Dilback

4. Remove VCC-Dilback fixing screws.

Installation
1. Verify the instrument is switched off and the power cable
is disconnected.
2. Ensure the BUS END jumper is present; it is located on the
rear of the board at the lower-left corner.
3. Install in reverse order of removal.

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200942-103—September 2007
Section 9 Dilutors

Verification
Ensure operating readiness by performing the following:
• CAN-BUS Resistance Test on page 4–33
• the following M&D checks:
– 50 µL Dispense Test
– 800 µL Dispense Test
See Section 5, Maintenance and Diagnostic Procedures,
for more information.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Level Sensors
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

Overview
There are three unique level sensors on the instrument:
• system liquid
• system waste
• solid waste

Removal
1. Switch off the instrument.
2. Disconnect the sensor in question from the SMIO board
• system liquid (J18)
• system waste (J19)
• solid waste (J21)

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Section 9 Level Sensors

Figure 9.203:Waste Sensor

3. Remove the two screws securing the level sensor to its


bracket
NOTE: Sensor position B is the sensor position
that is affected by using the photoelectric sensor.

Liquid waste
sensor

System liquid
sensor

Figure 9.204:Liquid Sensors

4. Remove the sensor and cable from the instrument.

Figure 9.205:Solid Waste Sensor

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Repair, Replacement of Spare Parts Section 9

Installation
1. Install the sensor onto the bracket and secure with two
screws.
2. Route the cable up through the hole in the cabinet,
between the instrument and cabinet, over to the right
access door, and up to the SMIO board.
3. Plug the sensor cable into the appropriate connector.
• The system liquid sensor cable is labeled in green.
• The liquid waste sensor cable is labeled in red.

– J18 system liquid


– J19 system waste
– J21 solid waste

Figure 9.206:SMIO-SAFY

Sensor Adjustment and Calibration


Adjustments and calibrations are performed through the S&S
software. Refer to Calibrate and Verify System Liquid, Liquid
Waste, and Solid Waste Sensors on page 4–105.

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200942-103—September 2007
Section 9 Door Locks

Door Locks
Removal
1. Switch off the instrument.
2. Open the front access panel and front worktable cover.
3. Loosen one screw (M3) securing the door lock.
4. Disconnect the cable at the door lock or disconnect the
door lock cable at the SMIO-SAFY board.

Figure 9.207:Door Lock

Figure 9.208:SMIO-SAFY Board

5. Remove the door lock

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
1. Open the front access panel.
2. Insert the door lock into position.
3. Finger tighten the socket head screw.
4. Connect the cable at the door lock or at the SMIO-SAFY
board.
• J5/J24 left door lock
• J6/J23 right door lock
5. Align the door lock to the catch so the door closes
smoothly.
6. Tighten the screw on the door lock.
7. Arrange the cables in the duct so they are not bent.
8. Close the front access panel.

Verification
Verification is performed through the S&S software. Refer to
Check Door Locks on page 4–115.

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200942-103—September 2007
Section 9 1 mL Subsystem Assembly

1 mL Subsystem Assembly
CAUTION: Hot Surface. Heater zone 1 operates at a
temperature of 50°C and heater zone 2 operates at
75°C. Be careful of exposure to hot surfaces.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Complete 1 mL Subsystem Assembly

Removal
1. Switch off the instrument.
2. Disconnect the two 1 mL Subsystem cables from the 1 mL
Subsystem.

Figure 9.209:1 mL Subsystem

3. Carefully lift the 1 mL Subsystem from the worktable


surface.

Figure 9.210:1 mL Subsystem Cables

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Section 9 1 mL Subsystem Assembly

Installation
1. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
2. Connect the two cables to the two heater boards, accessed
through the notched opening in the bottom cover of the
1 mL Subsystem.

Verification: Heater Block Temperature Check


Verification is performed through the S&S software. Refer to
Verify Temperature Performance of 1 mL Subsystem Heater
Blocks on page 4–119.

1 mL Subsystem Heater Block

Removal
1. Switch off the instrument.
2. Remove the 1 mL Subsystem from the worktable.
3. Remove the racks from the 1 mL Subsystem.
4. Turn the 1 mL Subsystem upside down and place it on the
bench.
5. Remove the eighteen outer and four inner screws (M2)
that secure the bottom cover in place.

Figure 9.211:1mL Subsystem Bottom Cover

6. Remove the cover.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

7. Turn the 1 mL Subsystem over and remove the six larger


headed screws (M2.5) that hold the honeycomb-colored
cover in place over the heater block. Hold the heater block
in place to prevent it from falling.

Figure 9.212:Heater Block, Removed

8. Disconnect the heater cable.


9. Remove the heater block from the 1 mL Subsystem.

Installation
1. Place the new heater block in the 1 mL Subsystem.

Figure 9.213:Heater Block

2. Connect the heater cable to the heater block.


3. Attach the heater block to the 1 mL Subsystem frame with
six screws.

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Section 9 1 mL Subsystem Assembly

4. Turn the 1 mL Subsystem over and verify the black heater


cables are placed in the notches in the plastic dividers.
This ensures the cables are not pinched when the bottom
cover is installed.
NOTE: Some newer versions may have a clamp
that prevents the cable from becoming easily
disconnected from the connection.
5. Install the bottom cover, securing it in place with
twenty-two screws.
6. Place the rack back into the 1 mL Subsystem.
7. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
8. Connect the two 1 mL Subsystem cables coming from the
OPTIBO board to the 1 mL Subsystem.

Verification
Perform the 1 mL Subsystem Heater Block Temperature check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.

1 mL Subsystem Heater Boards


CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.

Removal
1. Switch off the instrument.
2. Remove the 1 mL Subsystem from the worktable.
3. Remove the racks from the 1 mL Subsystem.
4. Turn the 1 mL Subsystem upside down and place it on the
bench.
5. Remove the eighteen outer and four inner screws (M2)
that secure the bottom cover in place.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

6. Remove the cover.


7. Remove the four outer screws (M2), located on the end of
the 1 mL Subsystem closest to the opening in the bottom
cover, holding the heater boards in place.
8. Disconnect the two cables connecting the heater blocks to
the heater boards and remove the heater boards.

Figure 9.214:Heater Block Cable Connections

Installation
1. Ensure the board address is set to match the replaced
board. The address will be either 3 or 4.
2. Place the heater board assembly into the 1 mL Subsystem
and connect the two cables linking the heater blocks to
the heater boards.
3. Secure the heater board assembly to the side wall of the
1 mL Subsystem with four screws.
4. Install the bottom cover with four longer inner cover
screws and eighteen shorter perimeter screws.
5. Place the rack back into the 1 mL Subsystem.
6. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
7. Connect the two 1 mL Subsystem cables originating from
the OPTIBO board to the 1 mL Subsystem.

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200942-103—September 2007
Section 9 1 mL Subsystem Assembly

Verification
Perform the 1 mL Subsystem Heater Block Temperature Check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

1 mL Subsystem Internal Cables

Removal
1. Switch off the instrument.
2. Remove the 1 mL Subsystem from the worktable.
3. Remove the racks from the 1 mL Subsystem.
4. Unscrew and remove the bottom cover as previously
described.
5. Disconnect the heater cable from the heater block and
heater board.

Installation
1. Connect the heater cable from the heater block to the
heater board.
2. Verify the black heater cables are placed in the notches in
the plastic dividers. This ensures the cables are not
pinched when the bottom cover is installed.
3. Install the bottom cover as previously described.
4. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
5. Connect the two 1 mL Subsystem cables originating from
the OPTIBO board to the 1 mL Subsystem.

Verification
Perform the 1 mL Subsystem Heater Block Temperature Check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.

1 mL Subsystem Cables, from OPTIBO Board

Removal
1. Switch off the instrument.
2. Open the left access service door and disconnect the two
cables from CAN OPTION 3 and 4 on the OPTIBO board.

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200942-103—September 2007
Section 9 1 mL Subsystem Assembly

Figure 9.215:OPTIBO Board

3. Remove the two outer screws of the left worktable panel


that covers the FaWa assembly. Pull the FaWa assembly
partially out of the instrument—like a drawer—to access
the cables.

Figure 9.216:Worktable Side Panel

4. Lift the 1 mL Subsystem partially up and away from the


worktable, and disconnect the cables.
5. Open the front worktable cover and remove the sealing
strip enough to remove the cables.
6. Carefully remove the cables from below the instrument.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Installation
1. Route the 1 mL Subsystem cables through the notched
holes in the sealing strip, leaving enough length to
connect to the 1 mL Subsystem.
2. Route the cables underneath the worktable surface, along
the front edge of the FaWa assembly, and up to the left
access door.

Figure 9.217:1 mL Cables

3. Plug the cables into CAN OPTION 3 and 4 on the OPTIBO


board.
4. With the cables in place, carefully push the FaWa
assembly into place and secure the FaWa assembly to the
instrument frame with two screws.
5. Align the left edge of the 1 mL Subsystem with grid 7.
Place the 1 mL Subsystem on the deck, aligning the
notches in the middle guide bar on the bottom of the 1 mL
Subsystem with the guide pins on grid 12.
6. Connect the two cables to the 1 mL Subsystem.
7. Feed any extra cable length back underneath the
instrument.
8. Verify the sealing strip is in place then close the front
worktable cover.

Verification
Perform the 1 mL Subsystem Heater Block Temperature check.
Refer to Verify Temperature Performance of 1 mL Subsystem
Heater Blocks on page 4–119 for verification.

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200942-103—September 2007
Section 9 Chiller/Output Assembly

Chiller/Output Assembly
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic-discharge-
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body before
touching the instrument.
WARNING: Pinch Point. The instrument requires
accurate positioning of the system modules, and
carriers and racks on worktables. When operating the
instrument, it could be necessary to run the test with
the instrument’s safety panels open. Keep body parts
away from moving parts of the instrument (e.g., LiHa,
RoMa, tips, and gripper fingers) during setup and test
procedures.

• Stand back from the worktable surface during


these procedures.
• Fast moving devices could cause injuries.

Removal
1. Switch off the instrument.
2. Switch off the chiller using the on/off switch on the back
of the chiller, and disconnect the RS-232 communication
cable and the power supply cable from the back of the
chiller assembly

Figure 9.218:Chiller Power Switch

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200942-103—September 2007
Section 9

3. Carefully lift the chiller/Output Deck assembly from the


worktable surface.

Installation
1. Install the Output Deck on grid locations 35 - 41. Ensure
that the cooling assembly engages the deck pins securely.
2. Plug the RS-232 and power supply cable into their
respective mating connectors on the chiller assembly.
3. Turn the chiller assembly on using the on/off switch on
the back of the chiller.

Verification
1. Turn the chiller on.
2. Log into the system as FSE.
3. Perform the M&D Chiller and Heater Temperature Check
on page 5–52. See Section 5, Maintenance and Diagnostic
Procedures for more information

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200942-103—September 2007
Section 9 Hood Maintenance

Hood Maintenance
This section is applicable to hooded instruments only. Most
hood maintenance is performed using the latest revision of the
hood repair ISA, which includes procedures for all applicable
field replaceable parts.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

CAN-BUS Resistor Test


After exchange or new installation of one of the following
boards, a CAN-BUS resistor test must be performed.

BOARD TEST

DILBACK (Dilutor Backplane)

BACKPLANE Liquid handling arm (LiHa) System CAN

CU-Board

OPTIBO

CU-Board

CU-Board PosID V2

OPTIBO

CC MAGS #3

CC MAGS #4 Option CAN

MPO

Backplane Robotic Manipulator Arm

SMIO

SMIO SAFY

1. Switch off the instrument.


2. Open the left service access door.
3. Depending on which boards were changed, check the
CAN-BUS resistor with an ohmmeter on the two
checkpoints on the OPTIBO as described below.

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200942-103—September 2007
Section 9 CAN-BUS Resistor Test

Quick Check for Jumper Settings


Measure the CAN-BUS resistance on the OPTIBO (left access
door).

Figure 9.219: CAN-BUS Resistance on OPTIBO

Table 9.3: Jumper Settings

CAN-BUS Check Points Resistance Interpretation

System CAN_L/ 50 to 65 ⏲ Correct number of


CAN-BUS CAN_H jumpers
~ 40 ⏲ 1 jumper too many
~ 120 ⏲ 1 jumper too few
Option CANO-L/ 50 to 65 ⏲ Correct number of
CAN-BUS CANO-H jumpers
~ 40 ⏲ 1 jumper too many
~ 120 ⏲ 1 jumper too few

NOTE: If the value is incorrect, check relevant boards


and the number of jumpers.
4. Remove the ohmmeter then close and lock the left service
access door.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Table 9.4: System and Option CAN Bus Jumper Settings


Jumper Location
CAN-BUS Present

SYSTEM CAN
DILBACK Yes J10 (not labeled)
LiHa Yes J14
Backplane
CU Board No J1
OPTIBO No SW1 off

OPTION CAN
CU Board No J22
PosID 2 No J6/J8
OPTIBO No SW2 off
CC MAGS #3 No no jumper
CC MAGS #4 No no jumper
MPO No J2
RoMa Yes J2
Backplane
SMIO Yes J9 outer two pins
SMIO/SAFY No J9 inner two pins

Movement Check
Manually check movement of all (system) modules.

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200942-103—September 2007
Section 9 Return of Parts

Return of Parts
Return of Parts to Abbott Molecular
Before an Abbott FSE performs any work on the instrument, or
before the instrument or any part is returned to Abbott, the
owner of the instrument must confirm in writing that
decontamination was performed properly and in accordance
with good laboratory practice guidelines. For this, the owner
must enclose a Decontamination Form. Refer to the Field
Decontamination Checklist on page 2–15.

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200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Service Checklist
This form is a test requirement checklist for the FSE. Refer to
the m2000sp Service Manual for additional tests that might
be applicable for a specific replaced assembly or component.

Parts/Components Replaced

Gravimetric Test (50 or 800 µl)


S&S Ref. Posns. (LiHa/RoMa)

RoMa Deck and Shelf M&Ds


Heater Block Temp Check

Chiller/Heater Temp Test

RoMa 1mL Carrier M&D


Sample Extraction Test
CAN-BUS Resist Test
Lower DiTi Eject Test
Liquid Detection Test

Reference Plate Test

Z-Calibration Check
RoMa Mech. Adjust
Random Move Test

PosID Adjustment
FaWa Pump Test
Tightness Check

Door Lock Test


Sensor Test
PosID Test
CU PCB X X X X X X X X X X X X X

OPTIBO PCB X X X X X X X X

Power Supply X X

X-drive, X-slide, X-belt X X

LiHa Arm X X X X X X X X

LiHa Arm adjusted X X X X

LiHa Arm Belts X X X

LiHa Flex Cable X X X X

LiHa DC-Servo PCB X X X X X X X

LiHa DC-Servo Power PCB X X X X X X

LiHa Y-motors X X X

ILID Cable X

ILID VCC PCB X

ILID Board Flat Cable X X X X X

LiHa Backplane PCB X X X X X X

Tip Adapters X X X X X

DiTi Adapter (spare) X X X X X

RoMa Arm X X X X X X X

RoMa Arm aligned X X X X X X

RoMa Grippers X X X

Chiller X X

RoMa Arm Belts and Flex Cable X X X X X X

9-210 m2000sp G-series Service Manual


200942-103—September 2007
Section 9 Service Checklist

Parts/Components Replaced

Gravimetric Test (50 or 800 µl)


S&S Ref. Posns. (LiHa/RoMa)

RoMa Deck and Shelf M&Ds


Heater Block Temp Check

Chiller/Heater Temp Test

RoMa 1mL Carrier M&D


Sample Extraction Test
CAN-BUS Resist Test
Lower DiTi Eject Test

Reference Plate Test


Liquid Detection Test

Z-Calibration Check
RoMa Mech. Adjust
Random Move Test

PosID Adjustment
FaWa Pump Test
Tightness Check

Door Lock Test


Sensor Test
PosID Test
RoMa Rotator Motor, Gripper X X X X X X
Board

RoMa Y/R-, Z/G- DC- Servo X X X X X X


Motor PCBs

RoMa Y, Z Motors X X X X X X

RoMa Z-brake X X X X X X

RoMa Freedom Backplane X X X X X X X

RoMa CU PCB X X X X X X

RoMa X-DC-Servo Power PCB X X X X X X

RoMa 2 Backplane PCB X X X X X X X

PosID X X X X

PosID 2CU PCB X X X X

X-drive Assy (belt, motor, cable) X X

X-DC-Servo PCB X X X

Y/B PCB, Y/B-DC-Servo PCB X X

DSP Decoding PCB X X X

Scanner Assy (B-motor, head, X X X


and cable)

Y-drive Assy (Y-belt, gripper, X X


Y-motor)

No Tube Sensor X X X

Dilback VCC PCB X X

Dilutor X X X X X

Syringe X X X

3-way Valve X X X X

Pipetting Tubing X X X X

Interconnecting Tubing X X X X

Aspirating Tubing X X X X

m2000sp G-series Service Manual 9-211


200942-103—September 2007
Repair, Replacement of Spare Parts Section 9

Parts/Components Replaced

Gravimetric Test (50 or 800 µl)


S&S Ref. Posns. (LiHa/RoMa)

RoMa Deck and Shelf M&Ds


Heater Block Temp Check

Chiller/Heater Temp Test

RoMa 1mL Carrier M&D


Sample Extraction Test
CAN-BUS Resist Test
Lower DiTi Eject Test

Reference Plate Test


Liquid Detection Test

Z-Calibration Check
RoMa Mech. Adjust
Random Move Test

PosID Adjustment
FaWa Pump Test
Tightness Check

Door Lock Test


Sensor Test
PosID Test
Fast Wash Pump X X

Fast Wash Pump MPO PCB X X X

Fast Wash (MPO/FWO) Assy X X X

Cable to FaWa PCB X X

Pressure-Relief Valve X X

Tubing to Fast Wash Pump X X

Tubing to Pressure-Relief Valve X X

Worktable X X X X X

Worktable guide pins X X X

Door Locks X

System Liquid Sensor X

Liquid Waste Sensor X

Solid Waste Sensor X

SMIO/SAFY X X

SMIO X X

1 mL Subsystem Assy X X X X

1 mL Subsystem Heater Block X X

1 mL Subsystem Heater Boards X X

1 mL Subsystem Internal Cable X X

1 mL Subsystem OPTIBO Cable X X

Lower DiTi Eject Option X

Solenoid X

DC-DCPower Board X

9-212 m2000sp G-series Service Manual


200942-103—September 2007
Section 10: Shutdown, Storage, and Shipping

SECTION 10:
SHUTDOWN, STORAGE,
AND SHIPPING

m2000sp G-series Service Manual 10-1


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

NOTES

10-2 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-4
When to Decontaminate . . . . . . . . . . . . . . . . . . . . . . . . . . 10-5
Decontamination Method . . . . . . . . . . . . . . . . . . . . . . . . . 10-6
Instrument and Cabinet . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-7
SCC and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-13
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-14

m2000sp G-series Service Manual 10-3


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

Purpose of This Section


The following procedure is performed to prepare a system for
relocation. The FSE prepares the instrument for shipment.
Observe the following safety notices:

WARNING: Potential Biohazard. Indicates an area


where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazards exist if parts of the instrument
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eyewear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Laboratories sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eyewear.

10-4 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 When to Decontaminate

When to Decontaminate
In addition to regular decontamination, the user must
thoroughly decontaminate the instrument according to
standard laboratory regulations in the following cases:
• before any maintenance or service is performed on the
instrument
• before an FSE performs on-site service on the instrument
• before the instrument is returned (e.g., for repair)
• prior to storage of the instrument
• prior to disposal of the instrument or parts of it
• in most cases, before the instrument or individual parts
leave the user’s site

m2000sp G-series Service Manual 10-5


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

Decontamination Method
The decontamination method must be adapted to the
respective application and associated substances used by the
operator. The owner assumes full responsibility for appropriate
decontamination of the entire instrument.
Refer to the m2000sp Operations Manual for specific
decontamination instructions, and refer to the Field
Decontamination Checklist on page 2–15 of Section 2 in this
manual.
WARNING: Potential Biohazard. Contamination
hazards exist if parts of the instrument are not
completely decontaminated. Components with direct
contact (or the potential for direct or accidental
contact) with chemical or biological material must be
treated, including the tubing system and all upstream
equipment.

10-6 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 Instrument and Cabinet

Instrument and Cabinet


Perform the following steps prior to shipping/relocating the
instrument or prior to long-term shutdown (more than two
months).
NOTE: For long-term shutdown, the instrument and
hood (if applicable) do not need to be taken off the
cabinet and recrated, but all other steps should be
followed.
1. Disconnect system liquid tube from the system liquid
bottle and flush remaining liquid from the system before
performing the following procedure.
2. Power off the m2000sp. Unplug the m2000sp from the
power source (UPS or outlet).
3. Manually move the LiHa over the wash/waste station at
grid 18, and position the DiTis over the liquid waste chute.
4. If installed, remove and recrate the hood per TSB 605-018.
5. Open the upper access door on the top of the instrument
and unscrew and loosen each of the tubing connections at
the 1:4 manifolds.
6. Secure the tubing to the top of the instrument with tape.
7. Allow the water to drain completely out of the dispense
tubing.
8. Open the FaWa assembly door on the side of the m2000sp
and disconnect the tubing from the FaWa pump to allow
the fluid to drain.
9. It is the responsibility of the owner to dispose of solid and
liquid waste according to local regulations. Disconnect
and remove the solid waste container, and liquid waste
and system liquid bottles.
Clean the system liquid and liquid waste containers as
instructed in Clean Liquid System Procedures on page 8–8
of Section 8.
10. Remove all racks and carriers from the worktable surface.
11. Disconnect the two cables connected to the 1 mL
Subsystem and remove the 1 mL Subsystem from the
worktable surface. Remove the sealing strip and push the
two cables under the instrument.

m2000sp G-series Service Manual 10-7


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

12. Coil the 1 mL Subsystem cables, secure with tape or cable


ties, and place on the FaWa plate.
13. Disconnect the liquid and waste sensor cables from the
SMIO board.
14. Pull the sensor cables back to the sensors and secure to the
frame with tape or cable ties.
15. Disconnect the RS-232 cable from both the Optibo board
and SCC.
16. To protect the positioning pins, place two cardboard strips
between two rows on the worktable.

Figure 10.1: Cardboard Protection Strips

17. Install the left and right support brackets using two screws
to secure each bracket.

10-8 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 Instrument and Cabinet

Figure 10.2: Support Bracket

18. Carefully move the PosID arms to the left.


19. Support the PosID by installing styrofoam pieces.

Figure 10.3: Styrofoam Supports

20. Carefully move the LiHa and RoMa arms to the left.
21. Support the LiHa and RoMa with styrofoam pieces.

m2000sp G-series Service Manual 10-9


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

Figure 10.4: Styrofoam Supports

22. Wedge a styrofoam block between the diluters and hotel,


and place two styrofoam blocks on the top ends of the
m2000sp as shown below.

Figure 10.5: Styrofoam Support Placement

10-10 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 Instrument and Cabinet

23. With the assistance of others, carefully lift the m2000sp off
the cabinet and place it on a pallet. Use the lifting handles
to lift the m2000sp. Follow the instructions in Lifting the
Instrument on page 4–22 for proper use of the lifting
handles.
WARNING: The instrument is heavy. Four people
are required to carefully lift the instrument onto
the cabinet. While lifting, ensure the lift handles
remain engaged with the locking features of the
instrument.

Figure 10.6: Correct Placement on Pallet

m2000sp G-series Service Manual 10-11


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

24. Place the 1 mL Subsystem and accessories in the cardboard


accessories box and place the box inside the instrument
on the right side of the worktable.

Figure 10.7: Correct Placement of Boxed Components

25. Box the instrument. The shipper is responsible for


banding the package.

Figure 10.8: The Instrument Boxed

10-12 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 Instrument and Cabinet

26. Place any remaining accessories into the cabinet storage


area and secure with tape and styrofoam.
27. Box the cabinet. The shipper is responsible for banding the
package.

Figure 10.9: Cabinet and Instrument Ready for Shipping

m2000sp G-series Service Manual 10-13


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

SCC and Accessories

1. Power off the SCC, monitor, printer, and UPS. Disconnect


the power cords from the power source.
2. Disconnect all cables from the rear of the SCC.
3. Remove the monitor, keyboard, SCC, Edgeport™, and
printer from the computer cart and place them in packing
boxes. Pack associated cabling with each component.
4. Place the computer cart in a sturdy packing box.
5. Place the UPS in a sturdy packing box.

10-14 m2000sp G-series Service Manual


200942-103—September 2007
Section 10 Storage

Storage
• Use a cover to protect the instrument against dust and
debris, and store the instrument under the following
conditions:
– Storage Temperature: 1 to 60°C/34 to 140°F
– Storage Humidity: 5 to 80% relative (non-condensing)
at 울 30°C/86°F
• Store the instrument in its original packaging. Store all
manuals and logbooks with the instrument.

m2000sp G-series Service Manual 10-15


200942-103—September 2007
Shutdown, Storage, and Shipping Section 10

NOTES

10-16 m2000sp G-series Service Manual


200942-103—September 2007
Section 11: Genesis Setup and Service Software

SECTION 11:
GENESIS SETUP AND
SERVICE SOFTWARE

m2000sp G-series Service Manual 11-1


200942-103—September 2007
Genesis Setup and Service Software Section 11

NOTES

11-2 m2000sp G-series Service Manual


200942-103—September 2007
Section 11 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-7
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-8
PC Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-8
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-8
Firmware Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-9
Setup & Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-10
Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-10
Starting and Finishing Setup and Service . . . . . . . . . . 11-10
Password Protection . . . . . . . . . . . . . . . . . . . . . . . . . . 11-11
Setup & Service Main Window . . . . . . . . . . . . . . . . . . 11-12
Standard Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-14
Move Pages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-17
Operating Conditions . . . . . . . . . . . . . . . . . . . . . . . . . 11-21
Normal/Simulation Mode . . . . . . . . . . . . . . . . . . . . . . 11-22
System Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-23
Password . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-24
COM Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-25
Initialize Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . 11-26
Load Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-26
Test Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-28
Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-29
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-30
Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-32
Print Specific Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-32
Exit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-33
View and Tools Functions . . . . . . . . . . . . . . . . . . . . . . . . 11-34
View Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-34
Instrument Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-35
Instrument Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-35
Basic Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-35
Serial Number Settings. . . . . . . . . . . . . . . . . . . . . . . . . 11-39
Firmware Download . . . . . . . . . . . . . . . . . . . . . . . . . . 11-41
EEPROM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-42
Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-44
Set X-drive Properties . . . . . . . . . . . . . . . . . . . . . . . . . 11-46
Set Machine Size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-47
Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-48
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-50
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-51
Command Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-52
Batch File Execution . . . . . . . . . . . . . . . . . . . . . . . . . . 11-53
Command Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-53

m2000sp G-series Service Manual 11-3


200942-103—September 2007
Genesis Setup and Service Software Section 11

Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-57
Results Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-58
Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-62
Version Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-63
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-64
System Devices Functions . . . . . . . . . . . . . . . . . . . . . . . . 11-67
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-67
System Devices Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-68
Positive Identification Functions for PosID 2 . . . . . . . . . 11-69
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-69
Mechanical Adjustment . . . . . . . . . . . . . . . . . . . . . . . 11-72
Bar Code Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-88
Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-91
Movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-101
Liquid Handling Arm (LiHa) . . . . . . . . . . . . . . . . . . . . . 11-103
LiHa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-104
Movement Axes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-105
Required Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-105
Before Starting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-105
Panel Description . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-106
Procedures and User Permission . . . . . . . . . . . . . . . . 11-107
Directory, Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-107
LiHa Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-108
Channel Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-110
Move LiHa Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-114
Setup Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-116
Set Default Parameters. . . . . . . . . . . . . . . . . . . . . . . . 11-118
Determine Range . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-118
Determine Reference Positions . . . . . . . . . . . . . . . . . 11-121
Determine Spacing . . . . . . . . . . . . . . . . . . . . . . . . . . 11-123
Individual Z Alignment . . . . . . . . . . . . . . . . . . . . . . . 11-124
Check Tip Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . 11-124
Calibrate the Lower DiTi Eject Protocol . . . . . . . . . . 11-125
Dilutor Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-126
Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-133
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-135
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-136
Move Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-138
Range Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-140
Random Move Test . . . . . . . . . . . . . . . . . . . . . . . . . . 11-141
Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-143
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-146
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-148
Robotic Manipulator Arm (RoMa) . . . . . . . . . . . . . . . . . 11-149
RoMa Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-155

11-4 m2000sp G-series Service Manual


200942-103—September 2007
Section 11 Table of Contents

Move RoMa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-158


Setup Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-159
Set Default Parameters . . . . . . . . . . . . . . . . . . . . . . . 11-160
Determine/Check Range . . . . . . . . . . . . . . . . . . . . . . 11-161
Set Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-163
Determine Reference Positions . . . . . . . . . . . . . . . . . 11-165
Adjust Rotator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-168
Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-169
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-171
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-172
Liquid System Functions . . . . . . . . . . . . . . . . . . . . . . . . 11-174
Liquid System Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-175
DiTi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-176
Lower DiTi Eject Test . . . . . . . . . . . . . . . . . . . . . . . . 11-177
Liquid Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-181
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-182
Normal Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-187
Fill/Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-194
FaWa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-195
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-196
Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-196
Options Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-206
Menu Items . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-206
Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-206
Access Status Options . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-207
Files, Directories . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-207
Access Status Options Panel . . . . . . . . . . . . . . . . . . . 11-208
Pages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-208
Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-208
Using the Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-209
Setup: Set Defaults in EEPROM . . . . . . . . . . . . . . . . . 11-209
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-210
Test: Device Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-210
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-211
Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-215
Test Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-216
Incubation Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-217
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-218
Burn-In of Heating Test . . . . . . . . . . . . . . . . . . . . . . 11-220
OPTIONAL Test: Heating Validation with TEMPO110 . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-221

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Genesis Setup and Service Software Section 11

SMIO for NAT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-223


Calibration of the DiTi Waste Sensor . . . . . . . . . . . . 11-223
Rapid Sensor Test . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-227
Accurate Sensor Verification . . . . . . . . . . . . . . . . . . . 11-229

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Section 11 Purpose of This Section

Purpose of This Section

This section describes installation of the G-series Instrument


software (Setup & Service software) for the m2000sp
instrument on a target computer. Information in this section
should be thoroughly reviewed before starting installation.
Refer to Section 4, Installation for an installation checklist
and Section 2, Safety for instrument and non-instrument
related hazards and safety measures.

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Genesis Setup and Service Software Section 11

Introduction
PC Requirements
Genesis Instrument software must be installed on a target
computer which is or will be connected to an m2000sp
instrument. The installation of the Genesis Setup and Service
software occurs during the installation of system software.

Installation
Refer to the latest software TSB and/or ISA for installation
instructions.

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Section 11 Firmware Compatibility

Firmware Compatibility
The Genesis Instrument software, Setup & Service was
validated with the firmware versions as defined in the
following table.
The following table lists firmware versions that were valid
when this manual was issued. It is possible some have
since changed. Contact your Abbott Representative for
the latest firmware list.
Table 11.1: Firmware Versions
File name
Device Download Firmware Comments
(--- = no file)
Genesis-CU Yes v1.63-12/2002 GENI163.hex Controls LiHa and
Control Unit machine
MPO Yes v2.00-11/99 MPO200.hex FaWa
Monitored Pump
Option
PosID 2 Yes v2.50-02/03 POS2_250.hex PosID 2
Positive
Identification
RoMa Yes v1.13-07/2002 dcuro113.hex RoMa on Device-CU
Robotic
Manipulator Arm
SAFY Yes v1.00-05/2001 ___sa100.hex SAFY
SMIO-SAFY Yes v1.01-11/99 SMIO101.hex SMIO-SAFY
VCC Yes v1.20-06/2000 vcc120.hex (for Dilutor type XP
Volume Control 3000 plus)
Center
MAGS Yes v1.30-01/2003 MAGS for 1 mL Subsystem
130.hex

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Genesis Setup and Service Software Section 11

Setup & Service


Purpose
This section describes the following:
• How to start the Setup & Service software
• The different password levels
• The GENESIS Setup & Service user interface
• General functions of the software
• The different operating modes
WARNING: Setup and calibration data affect the
performance of every instrument operation. Read this
manual when changing any of these settings, and
carefully follow instructions in relevant sections.
The m2000sp instrument or workstation was thoroughly
configured and tested at the factory site before it was shipped
to the customer. However, to check the settings after transport,
the setup procedure must be repeated after installation at
customers site to verify proper functioning of the m2000sp
instrument or workstation.

Starting and Finishing Setup and Service

Starting the Program


To start the Setup & Service software, log in to the application
as an FSE and navigate to <System><Setup and Service>.
The Setup & Service software starts with the GENESIS Setup &
Service Main window which displays an empty worktable.
NOTE: If there is no communication between the
computer and instrument, components could be
disconnected or communication is not working
properly. A message displays, prompting whether to
proceed. Check connections to the instrument!
The Genesis Instrument software uses standard Windows keys
and keyboard functions. The software can be controlled with
the mouse or keyboard. However, not all functions are
accessible to users without a mouse. There are no unique key
functions within Genesis Instrument software.

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Section 11 Setup & Service

Finishing the Program


Perform the following step to finish the Setup & Service
software.
Select <System><Exit>. The Setup & Service Main window
closes and communication to the instrument is disconnected.
The entire SCC will shut down. Power on the SCC to restart
the m2000sp application.

Password Protection

Purpose
Access to the FSE level of Setup & Service is password protected
to prevent unauthorized use of the Setup & Service module of
the Genesis Instrument software.

Access Levels
There are two access levels:
• Operators—Operators can perform tests and validation
procedure without password entry. If so, some menu items
and controls are disabled. Operators cannot perform setup
and calibration procedures that could affect instrument
performance if performed incorrectly.
• FSEs—Setup and calibration procedures require the FSE
password because of restricted access. In this manual,
these procedures include the following note:

Entering the Password


Enter the FSE password with <System><Password>. Refer to
Password later in this section.

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Genesis Setup and Service Software Section 11

Setup & Service Main Window

Overview
The Genesis Setup & Service Main window is divided into
four sections as illustrated below.

Toolbar can be switched on/off Menu Bar


with View > Tool Bar

Worktable (empty)

Status bar can be switched


on/off with View > Status Bar

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Section 11 Setup & Service

Menu Bar
The menu bar includes the following menus and commands.

Command Description
System System functions
View View functions
Instrument Instrument functions
System System devices
Devices
Liquid System Liquid system functions
Options Options 1 and 2, and Te-MO

Toolbar
The toolbar contains quick-access buttons for the most
frequently used functions.

1) 2) 3) 4) 5) 6)

Figure 11.1: Toolbar

Button Description
1) <System><Password>

2) <System><Initialize Instrument>

3) <System><Load Worktable>

4) <System><Test Configuration>

5) <System><About>

6) <<System><iew><Worktable><Zoom Active>

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Genesis Setup and Service Software Section 11

Standard Panel

Description
Many Setup & Service functions open a function panel that is
subdivided into several pages. The following illustration
displays the Access Status panel.

• There are one or more tabs at the top of the window to


allow selection of several functions.
• The list box Available Devices in the Contents page
displays the selectable devices. Refer to Device Selection
later in this section.
• Usually, there are several checkboxes below the list box.
Selection of one or more checkbox (聺) defines desired
options in the instrument software.
– All can be checked at once to perform all possible
setups and tests in one run.
– Checkboxes can be selected one at a time to perform
each setup or test individually.
– If selecting checkboxes individually, the setups/tests
should be selected in a logical order, i.e., the topmost
checkbox is selected first then proceed down the list
with selections.

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Section 11 Setup & Service

• The command buttons at the bottom are used to start and


stop selected actions, print reports, etc. Refer to
Command Buttons later in this section.

Device Selection
Available devices listed below are included in the Available
Devices section on the corresponding Contents page. The
behavior of the software depends on the number of connected
devices.

No of
Connected Behavior
Devices
0 The Start button is dimmed and the tabs are
visible.
1 The connected device is automatically
selected and its settings are read
automatically.
2 or more None of the connected devices are selected.
One device must be selected then its settings
are read.

Command Buttons
The command buttons near the bottom of the screen are used
to perform the desired action.

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Genesis Setup and Service Software Section 11

Button Description
Start Starts the desired procedure. This is possible:
- if a device is selected in the Available Devices
section.
- if at least one setup or test procedure is selected.
- if valid data were entered on the corresponding
pages.
- If more than one procedure is selected, they are
executed in the sequence as listed in the
Contents page. The topmost procedure is
executed first. After starting a procedure, Start
changes to Stop.
Stop Stops a running procedure. The procedures
executed are marked gray, those not executed are
black.
Execution can be resumed with Start if no settings
are changed.
Print Prints the test results or the Test report, if
implemented.
Save As Starts the standard Windows Save As dialog to
save the test configuration data in an appropriate
*.any file.
Enter the full file name (with extension.any).
Default Sets all parameters to their default values.
Undo Reverts to the previous settings.
OK Saves the defined values and closes the panel.
Cancel Closes the panel, but does not save any changes.
Close Closes the panel.
Apply Saves defined values, but does not close the panel.
The window remains open. After selecting Apply,
Cancel changes to Close and OK is dimmed.

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Section 11 Setup & Service

Move Pages

Description
Panels for some system devices and options that contain
movable parts (e.g., LiHa, RoMa) contain a Move page. The
Move page allows movement of the whole device or parts of it
in certain movement axes. The following example illustrates
the Move LiHa page.
NOTE: In this section, only the movement buttons are
described. For other controls on a specific Move page,
refer to the description of the respective system device
or option.

Movement Buttons
Depending on the parts that can be moved, a Move page
includes some or all of the following movement buttons.

Button Description
Single step, in 1/10 mm or 1/10 degree,
movement/rotation buttons.
Ten steps, in 1 mm or 1 degree,
movement/rotation buttons.

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Genesis Setup and Service Software Section 11

Button Description
Continuous movement/rotation buttons
when button is kept pressed.
Special buttons to move certain parts to
specific logical positions. Refer to the Move
page of the corresponding device for
details.

• Arrows on the movement buttons indicate the direction


that respective parts will move.
• If the mouse pointer is placed over a movement button,
text displays to explain the button’s function (“tool tip”).

Using the Keyboard


In addition to movement buttons, certain keys can also be
used to move parts as illustrated below.

Insert Home
Page Num
/ _
Up lock *

Delete End
Page 7 8 9
Down Home PgUp
+
4 5 6

1 2 3
End PgDn
Enter
0 .
Ins Del

Figure 11.2: Keyboard controls

Available keys are located:


• In the numeric keypad on the right side of the keyboard;
refer to NOTE below.
• To move LiHa and RoMa, keys in the key blocks to the left
of the numeric keypad can also be used.
NOTE: If keys of the numeric keypad will be used,
ensure the Num Lock is enabled; if there is a Num
Lock indicator lamp, it must be turned on.

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Section 11 Setup & Service

Directions
The following table lists the axes and directions in which the
devices (LiHa and RoMa) can be moved.

Key in
Key (Left of Movement/
Numeric Pad Movement
Numeric Rotation Axes
(Num Lock Axes LiHa
Keypad) RoMa
Switched on)
 6 X+ (right) X+ (right)
 4 X- (left) X- (left)
 2↓ Y+ front Y+ front
 8 Y- (rear) Y- (rear)
Page Down PgDn 3 Z+ (down) Z+ (down)
Page Up PgUp 9 Z- (up) Z- (up)
a
Home 7 Home R+ (CW) ---
End 1 End R- (CCW) a) ---
b G+ (increase) c
Delete Delete G+ (open)
Insert 0 Insert G- (close) b) G- (decrease) c)
+ --- ---
- --- ---
a. RoMa - Rotation of gripper head clockwise/counterclockwise
b. RoMa - Open/close grippers
c. LiHa - Increase/decrease spacing between tips

Steps
Movable parts of the listed arms or options are moved as
described below.
• Each time one of the above keys is pressed, the part is
moved/rotated by one step, 0.1 mm or 0.1 degree.
• If the key is pressed and held down, the part is
moved/rotated continuously at a speed of two steps per
second.
• With LiHa and RoMa, the key can be pressed continuously
with the Ctrl key pressed at the same time. If so, the arm
is accelerated until it reaches a maximum speed.

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Genesis Setup and Service Software Section 11

Result Files
For newer functions, each time a test procedure is started, a
result file is created and test results (all information as on the
Test reports) are written to the directories listed.

Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Results
File name: <function>_<serial_number>_<date>_<ti
me>.any
Example: LiHa_2209_29Mar2003_09-14-18.any

Log Files
Some functions create a log file to which measured values are
written.
Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Log
File name: Function dependent, refer to corresponding
section

Test Configuration Files (*.any)


If test configuration data are saved with Save As, an
appropriate *.any file is created and written to the following
directory.
Description
Saved in directory: C:\Program Files\Tecan\Genesis
Instrument Software\Service
File name: <name>.any
Example: MyPosID.any

• Existing *.any files can be loaded from the user-defined


directory with <System><Test Configuration>.
The file name displays in the corresponding <function> Panel
Windows title bar and printed in the Test report as Test
Configuration: User Defined [file name].

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Section 11 Setup & Service

Test Configuration Files (*.ini)


If Save is selected for test configuration data for older
functions, an appropriate *.ini file is created and written to
the directories listed.
Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Results
File name: <name>.ini
Example: MyPosIDTest.ini

• Load an existing *.ini file with <System>


<Test Configuration>.

Operating Conditions
Before operating Setup & Service, perform the following
verifications.
1. Ensure the correct firmware is downloaded; refer to
Firmware Compatibility, page 11-9.
2. To avoid movement crashes, verify the instrument is set
up with <Instrument><Basic Setup>.
NOTE: The correct worktable size is set
automatically when a worktable is loaded.
3. Some functions require an empty worktable free of all
obstacles. Observe the NOTES included in the
corresponding sections.
4. Do not start any function if the prerequisites or
requirements described are not fulfilled.

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Genesis Setup and Service Software Section 11

Normal/Simulation Mode

Operating Modes
The instrument software can run in two operating modes. This
section describes the normal mode of operation.

Normal Mode
This is the normal mode of operation of Setup & Service
software for all Genesis Instruments and workstations, with all
functions and menu entries enabled.

Simulation Mode
In the simulation mode, Setup & Service software can be run
without a physical instrument connected to the computer.
This mode allows telephone support personnel to simulate
certain features when no instrument is connected to their
computers. However, access to features is quite restricted in
this mode; therefore its use is not recommended.
If an instrument is not connected to the computer, the Setup
& Service software automatically starts in simulation mode.
Normal and simulation modes are possible for all supported
instrument types.

Switching to Normal Mode


To switch from Simulation back to Normal mode, ensure the
RS-232 cable is plugged in, power is on to the instrument, and
the port is set to COM 1.

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Section 11 System Functions

System Functions
Description
The System menu includes all commands that apply to the
whole system and not to a particular function.

Commands
The System menu includes the following commands.

Command Description
RMP Enables operation in RMP mode; refer to
Operating Conditions, page 11-21. Not
applicable with the m2000sp instrument.
Password For entering the FSE password; refer to
Password, page 11-24.
Initialize Prepares the instrument for usage by
Instrument initializing each axis. After switching on the
instrument, it must be initialized.
Load Loads a predefined or edited worktable map;
Worktable refer to Load Worktable, page 11-26.
Test Loads an *.ini or *.any configuration file;
Configuration refer to Test Configuration, page 11-28.
Print Setup Starts the standard Windows dialog to set up
a printer.
Print Prints test configuration and test results;
refer to Print, page 11-29.

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Genesis Setup and Service Software Section 11

Command Description
About Opens a window that displays copyright,
version, and other information related to
the Setup & Service software.
Exit Terminates the Setup & Service software;
refer to Exit, page 11-33.

Password

Purpose
To prevent unauthorized use of the Setup & Service module of
the Genesis Instrument software, access to the FSE level of
Setup & Service software is password-protected. No other
passwords are required. The FSE password is always the same
because there is not an available function to change the
password.

Procedure
Perform the following steps to enter the FSE password.
1. Start the function with <System><Password> or select the
icon on the toolbar. The Password dialog displays.

2. Enter the FSE password in the Enter Password field.


Confirm with OK. The FSE functions are enabled and
accessible in menus and screens.

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Section 11 System Functions

COM Port

Purpose
This function allows the communication port (e.g., COM 1, 2,
etc.) to be selected or changed. The computer communicates
with the instrument through the communication port.
NOTE: For m2000sp, the communication port should
always be set to COM1.

Procedure
Perform the following steps to select or change a
communication port.
1. Ensure the instrument is connected to the required COM
port connector, usually located on the rear side of the
computer. Consult the accompanying documentation of
the computer, if necessary.

Figure 11.3: COM Port Sub-menu

2. From the system COM port sub-menu, select the correct


port (COM 1, 2 etc.) The sub-menu lists all available COM
ports.
NOTE: In the sub-menu, the currently selected COM
port displays with a checkbox (聺).
NOTE: Depending on the selection, the computer
automatically leaves or enters the simulation mode, if
the instrument was connected to the “correct” COM
port before the simulation mode is entered and vice
versa.
NOTE: If the port does not save when set to COM 1
verify that the modem is not connected to COM 1.

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Genesis Setup and Service Software Section 11

Initialize Instrument

Purpose
This function initializes the instrument. During initialization,
all installed system devices (PosID, LiHa, and RoMa) are
moved to their initial positions:
• PosID RoMa and LiHa to the left side.

Procedure
Perform the following step to initialize the instrument.
1. Start the function with <System><Initialize Instrument>
or select the icon in the toolbar. The following message
displays:

The message box disappears when initialization is


complete.

Load Worktable

Purpose
To perform setups and tests, Setup & Service software requires
information about the location of carriers, racks, etc. on the
worktable. Tecan provides five predefined worktable maps
stored in the Genesis software database:
• NAT Worktable
– use for all m2000sp applications
• Default Worktable
• Empty Worktable
• Field Worktable NPS
• Liquid System Check Worktable
Additionally, Abbott provides the m2000sp worktable.

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Section 11 System Functions

Procedure
Perform the following steps to load a worktable map.
1. Start the function with <System><Load Worktable> or
select the icon in the toolbar. The Select Worktable
dialog displays.

Figure 11.4:Select Worktable Dialog

2. Select the desired worktable (m2000sp or NAT MBS).


NOTE: For any instrument using m2000sp
Software Version 2.0 or higher, use the m2000sp
Worktable.
3. Load the worktable map with OK. The selected map loads
and displays on the screen.

Figure 11.5: m2000sp Worktable

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Genesis Setup and Service Software Section 11

Figure 11.6:NAT MBS Worktable

Test Configuration

Purpose
The Test Configuration function loads a *.any or *.ini
configuration file.
NOTE: Never alter configuration files. They contain
configuration specific data to perform all instrument
tests.
NOTE: For the m2000sp instrument it is not necessary
to load the Service.ini file because the file is loaded by
default.

Procedure
Perform the following steps to load a *.any or *.ini file.
1. Start the function with <System><Test Configuration>
or select the icon in the toolbar. The Open
Configuration dialog displays.
2. To load an *.any file, select the file type in the Files of
type list.
3. Select the appropriate file.
4. Load the file with Open.
• *.ini configuration files are normally located in the
C:\Program Files\Tecan\Genesis Instrument
Software\Service directory.
• *.any configuration files are saved in a user-defined
directory.

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Section 11 System Functions

Print

Print Sub-menu
The Print sub-menu includes the following sub-menu
commands.

Command Description
Configuration Prints the configuration data stored in the
appropriate *.ini file; refer to Configuration,
page 11-30.
Test Prints test results of tests performed with the
instrument; refer to Test, page 11-32.

For older functions, specific configuration data or test results


can be printed by selecting Print for the corresponding
function (Print Specific Data, page 11-32).
Genesis Instrument software uses standard Windows printer
functions.

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Genesis Setup and Service Software Section 11

Configuration

Purpose
This function prints the configuration data stored in the
appropriate *.ini file. It opens the dialog displayed on the left.
The Common Print Information dialog includes the
following controls.

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Section 11 System Functions

Control Description
Destination
Printer Checkbox to select the printer for output
destination.
File Checkbox to select a file for output
destination.
After selecting OK, the standard Windows
Save As dialog displays. Select the desired
folder and enter a file name. Save the
output with Save.
Header Fields for free text. The entries are not
Information checked against a database.
Operator Operator ID for header information.
Option x Option serial number x of the devices
Serial No currently used, e.g., the serial number of a
replaced device.
OK Starts printing.
Cancel Aborts the function without printing.

Procedure
Perform the following steps to print all configuration data.
1. Start with <System><Print><Configuration>. The
Common Print Information dialog displays.
2. Select the print destination(s) and enter the Header
Information.
3. Start immediate printing with OK.

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Genesis Setup and Service Software Section 11

Test

Purpose
This function prints the results of tests performed with the
instrument.

Procedure
Perform the following steps to print the results of tests
performed with the instrument.
1. Start with <System><Print><Test>. The Common Print
Information dialog displays.
2. Select the print destination(s) and enter the Header
Information. Refer to Configuration, page 11-30.
3. Start immediate printing with OK.

Print Specific Data


Perform the following steps to print configuration data, and/or
test results of a specific older function after any test was run.
1. Select Print on the corresponding screen. The Common
Print Information dialog displays.
2. Select the print destination(s) and enter the Header
Information; refer to Configuration, page 11-30. Confirm
entries with OK. The Test Specific Print Information
dialog displays.

Figure 11.7: Test Specific Print Information Dialog

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Section 11 System Functions

The Test Specific Print Information dialog includes the


following controls.

Control Description
Print
Configuration Checkbox to select the configuration
data to be printed.
Detailed Checkbox to select detailed test
Results results to be printed. If the Detailed
Results checkbox is not selected, the
results printed will only be Test
passed or Test failed.
Comments Field for free comments.
OK Starts printing.
Cancel Aborts the function without
printing.
Help Provides help.

3. Select the data to be printed and enter any comments in


the Comments field.
4. Start immediate printing with OK. The Test Specific Print
Information dialog closes and the original screen is active
again.

Exit
Perform the following steps to finish the Setup & Service
software safely without data loss.
Select <System><Exit>.
Because changes to setup or test configurations are applied
immediately when selecting Apply or OK, there is no need for
<File><Save> or similar function in the Setup & Service
software.

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Genesis Setup and Service Software Section 11

View and Tools Functions


View Menu
The View menu includes the following commands.

Command Description
Worktable Refer to Figure 11.8 below.
Tool Bar Displays/hides the toolbar of the Main window.
Status Bar Displays/hides the status bar at the bottom of
the Main window.

The Worktable sub-menu includes the following three menu


commands.

Figure 11.8: Worktable Sub-menu

Command Description
Carrier Displays/hides carrier names in the worktable
Names map.
Rack Displays/hides rack names in the worktable
Names map.
Zoom Zooms the worktable map.
Active

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Section 11 Instrument Functions

Instrument Functions
Instrument Menu
The Instrument menu includes the following commands.

Command Description
Basic Setup Sets up an instrument (initial setup); refer to
Basic Setup, page 11-35.
Command Allows for use of single commands or
Tool execution of batch files.
Information Provides detailed information about
installed system devices and options; refer
to Information, page 11-57.

Basic Setup

Purpose
The Basic Setup panel allows setup of the m2000sp instrument
for further use (initial setup). This setup must be performed in
the following cases:
• To set X-drive properties, e.g., changing a CU board
without restoring parameters stored in EEPROM.
• When new firmware will be downloaded.
• To backup or restore parameters stored in EEPROM.
• When serial numbers will be configured.
• To define possible cutouts in the worktable.

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Genesis Setup and Service Software Section 11

Basic Setup Panel


The Basic Setup panel provides several procedures to be
executed. Generally, it is necessary to perform the following
steps.
1. Start the panel with <Instrument><Basic Setup>.
2. Select the setup procedures to perform and modify the
corresponding settings.
Proceed in one of the following two ways:
• Select ALL Setup checkboxes at once and enter
necessary data on the corresponding pages. Select
Start and all setup procedures are performed in one
run.
• Select one or more Setup checkboxes and define the
necessary data as described. Select Start to perform
the currently selected setup procedures.
• If the second method is selected, start with the
topmost setup procedure and work downward. Use
the second method to repeat an individual setup.
Before starting any setup procedure, the Operator field
on the Printing page should be completed.
3. Start the desired procedure(s) with Start.
4. After performing any setup, consult the Information page
Information, page 11-48.
5. Print the setup results.
6. Finish with Close.

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Section 11 Instrument Functions

Functions and User Permissions


Refer to
Operator FSE
Section
Serial Number Setting X page 11–39
Firmware Download X page 11–41
EEPROM Backup/Restore X page 11–42
Worktable X X page 11–44
Set X-drive Properties X page 11–46
Set Machine Size X 11–47
Information X X page 11–48
Printing X X page 11–50
Test Report X X page 11–51

Directory, Files
The Basic Setup function can create the following files (refer
also to Setup & Service, page 11-10).

Description
Saved in C:\Program Files\Tecan\Genesis
directory Instrument Software\Results
File name BasicSetup_<serial_number>_<date>_<tim
e>.any

Pages
The Instrument Basic Setup Panel is subdivided into the
following pages.

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Genesis Setup and Service Software Section 11

Page Description
Contents General overview, procedure selection.
Serial Number Serial number data; refer to Serial Number
Settings Settings, page 11-39.
Firmware Configuration of the firmware to be
Download downloaded; refer to Firmware Download,
page 11-41.
EEPROM Backs up/restores contents of EEPROM; refer
to EEPROM, page 11-42.
Worktable Provides information about the worktable
layout; refer to Worktable, page 11-44.
Set X-drive For setting up the X-drive; refer to Set
Properties X-drive Properties, page 11-46.
Information Displays instrument properties, diagnostic
data, and device information; refer to
Information, page 11-48.
Printing Print selection for the Test report; refer to
Printing, page 11-50.

The Instrument Basic Setup Panel also includes the standard


command buttons. Refer to Standard Panel in this section for
details.

Controls
The Contents page includes several checkboxes for selection
(or omission) of the following procedures,

Checkbox Description
Serial Number Displays the serial number of all installed
Settings devices; refer to Serial Number Settings,
page 11-39.
Firmware Displays and downloads firmware for
Download installed devices; refer to Serial Number
Settings, page 11-39.
EEPROM Backs up/restores EEPROM parameters; refer
to Serial Number Settings, page 11-39.
Set X Drive Determines the order of the arms and arm
Properties type at each position, and sets the default
offset and displacement for each arm; refer
to Serial Number Settings, page 11-39.

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Section 11 Instrument Functions

Checkbox Description
Set Machine Determines the size of the instrument; refer
Size to Serial Number Settings, page 11-39.

Using the Basic Setup Panel


Perform the following steps to set up the m2000sp instrument.
1. Start the panel with <Instrument><Basic Setup>.
NOTE: The Instrument Basic Setup panel with
activated Contents page displays. After starting
the Basic Setup function, no checkboxes are
selected and not all tabs are visible.
2. Select the setup procedures to be performed, and define
necessary data as described in the appropriate section. All
setup procedures can be selected all at once, or one or
more at a time.
3. Before starting any setup procedure, switch to the
Printing page to complete the Operator and Comment
fields as desired.
4. Start the selected procedures with Start.
5. Perform one of the following:
• Switch to the Information page to view setup
information (Information, page 11-48).
• Switch to the Printing page to preview the Test report
(Printing, page 11-50).
6. Finish with Close.

Serial Number Settings


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Serial Number Settings procedure displays serial numbers
for the installed devices. If no serial number is set for a device
(exception), it can also be entered.

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Procedure
Perform the following steps to display serial numbers.
1. Select the Serial Number Settings checkbox (聺) and
switch to the Serial Number Settings page.

The Serial Number Settings page includes the following


control.

Control Description
Set for Checkboxes to select device addresses for
which the serial number will be set.

2. If no serial number is set for a device (0 displays instead of


a serial number), select the corresponding Set for
checkbox (聺) and type the serial number in the respective
field in the Serial Number column.
NOTE: Incorrect serial numbers are accepted by the
system and cannot be changed at a later time.
3. Save the entries with Start.

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Section 11 Instrument Functions

Firmware Download
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Firmware Download procedure displays the current
firmware versions for the installed devices and downloads a
new or updated firmware version to the instrument.
Before starting the procedure refer to Firmware Compatibility
in this section to verify firmware compatibility.

Procedure
Perform the following steps to display the current firmware
version and to download a new one.
1. On the Contents page, select the Firmware Download
checkbox (聺) and switch to the Firmware Download
page.

Devices with firmware that cannot be downloaded do not


display on the Firmware Download page.
The Firmware Download page includes the following
controls.
Control Description
Do for Checkboxes to select the device address of
the firmware to download.
Replace List boxes to select the firmware files to
with download.
Browse Command buttons that start the standard
[…] Windows Open dialog to search the file to
download in a directory other than
C:\Program Files\Tecan\Genesis
Instrument Software\Firmware.

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Genesis Setup and Service Software Section 11

2. Perform the following to select new firmware:


• Select the corresponding Do for checkbox (聺).
• Select the firmware file from the Replace with list or
use the Browser […] button) to search the file.
3. Select Start to download the file.
4. If the message Please switch instrument off and on
displays:
• Switch off the instrument.
• Wait several seconds then switch it on again.
• Select OK at the Setup: Done prompt.

EEPROM
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The EEPROM function allows backup on disk of EEPROM
parameters of one or more devices, and/or restore such
parameters from disk. For example, backup of current device
parameters is recommended if an electronic board is replaced.
After the board is replaced, parameters can be restored so setup
or calibration procedures need not be performed again.

The EEPROM page includes the following controls.

Control Description
Do for Checkboxes to select the device addresses of
the EEPROMs to backup.

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Section 11 Instrument Functions

Control Description
Restore with Displays the names of the files selected with
the Browse […] buttons.
Browse […] Command buttons that start the standard
Windows Open dialog to search the files with
parameters to restore.

Backup
1. On the Contents page, select the EEPROM
Backup/Restore checkbox (聺) and switch to the
EEPROM page. The EEPROM page displays.

2. Select the checkboxes (聺) of the EEPROMs to be backed


up.

3. Select Start. Contents of the selected EEPROMs are written


to disk.

Restore
Perform the following steps to restore the contents of a file
previously backed up.

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1. Select the checkboxes (聺) of devices that need parameters


restored.
2. Use the appropriate browser buttons […] to search the files
that need parameters restored. Names of the selected files
display in the Restore with column.
3. Select Start. When Start is selected, the following occurs:
• Before restore is started, a backup copy is made of the
EEPROM contents of each selected device. Backup
data are written to the C:\Program
Files\Tecan\Genesis Instrument Software\EEPROM
directory.
• Contents of files displayed in the Restore with
column are restored to the associated EEPROMs, i.e.,
written back into the respective EEPROMs.

Worktable
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Worktable page is a tool that allows definition of possible
cutouts positions in worktables of the following instruments:
• m2000sp instrument
• Cutout left: none
• Cutout right: none

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Section 11 Instrument Functions

• Cutout board: not selected

The Worktable page includes the following controls.

Control Description
Upper part Picture that illustrates the worktable.
Selected cutouts display in the picture.
Frame Cutout Set of option buttons for definition of the
left cutout type on the left machine side.
None No cutout on this side.
Centrifuge Cutout for centrifuge below worktable.
Reader Cutout for reader below worktable.
Frame Cutout Set of option buttons for definition of the
right cutout type on the right machine side.
Refer above.
Frame Cutout Checkbox to select a cutout at the right
border edge of the worktable.

• Elements on the page cannot be activated if no cutouts are


possible for the specific instrument.
• Before using the Worktable page, define the machine size
with Set Machine Size.

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Procedure
Perform the following steps to define cutouts in the
Worktable.
1. From the Main menu, select <Instrument><Basic Setup>.
2. If not previously done, set the machine size; refer to Set
Machine Size, page 11-47.
3. Select the Worktable tab. The Worktable page displays.
4. Use the option buttons and/or checkboxes to define the
m2000sp worktable.
• Cutout left: none
• Cutout right: none
• Cutout board: not selected
5. Select the appropriate command button to store or discard
the selections:
• Apply: selections are stored, the panel remains open.
• OK: selections are stored, the panel is closed.
• Cancel: discards selections and closes the panel.

Set X-drive Properties


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

This procedure is only performed when an arm, such as the


LiHa or RoMa, is replaced or a CU board is replaced and
EEPROM data are not restored.

Purpose
The Set X Drive Properties procedure determines the order of
the arms and arm type at each position, and sets the default
offset and displacement for each arm.

Before Starting
• Ensure the worktable is empty and free of obstacles at all
times.
• Verify each arm can be initialized.
• If the X-drive properties are set, the LiHa and RoMa setup
function must be performed afterward.

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Section 11 Instrument Functions

Procedure
Perform the following steps to set the X-drive properties.
1. On the Contents page, select the Set X Drive Properties
checkbox (聺). No other parameters are selected.
2. Start the procedure with Start.
3. At the end of the procedure, the values found are written
to the instrument.

Set Machine Size


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

This procedure should only be performed if the instrument is


not set at 150.

Purpose
The Set Machine Size procedure detects the instrument size or
worktable length.

Procedure
Perform the following steps to determine the machine size.
1. On the Contents page, select the Set Machine Size
checkbox (聺). No further parameters need to be defined.
2. Start the procedure with Start.
3. At the end of the procedure, the values found are written
to the instrument.

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Information

Purpose
The Information page summarizes current instrument
properties, diagnostics data, and device information.

Using the Page


1. Switch to the Information page.

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The Information page displays the following data:

Description
Type Instrument type, e.g., Classic, Freedom

Instrument
Properties
Size Instrument size—100, 150, or 200 [cm]
Pos Position of arm installed—left to right
Arm Arm installed
Addr Address of arm
Power Ups Number of times the instrument was
switched on since first startupa
On Time Total power on time in hours
Diagnostics

RS-232 Host Connection to CU board


Error - Interrupts since last power up
interrupts - Overall (total number of interrupts since
first startup)a
Repeated - Repeated messages since last power up
messages - Overall (total number of messages since first
startup)a
Device Name Device name, e.g., LiHa, RoMa, etc.
Device Info

Addr Address of device


Firmware Current firmware version
Bootware Current bootware version
Serial Serial number
Number
a. first startup = time when instrument was run the first time

2. Print the setup results with Print or preview the Test


report (Printing, page 11-50).

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Printing

Purpose
The Printing page allows configuration of reports to meet
laboratory requirements.

Figure 11.9: Printing Page

Controls
The Printing page includes the following controls.

Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Instrument Checkbox to select instrument
Properties properties according to the
Information page.
Diagnostics data Checkbox to select the diagnostics
data displayed on the Information
page.
Multiple existing Data selection valid for all device data
Sections selected in the Detailed Print Section
Configuration section.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Allows preview of the report.

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Section 11 Instrument Functions

Control Description
Printer Setup Starts the standard Windows dialog to
set up a printer.
Use Paper Print in selected orientation.
Orientation of
Document

Print a Report
Perform the following steps to configure and print a report.
1. Switch to the Printing page (Figure 11.9).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the report onscreen with Result Preview.
4. Print the Test report with Print.

Test Report
Depending on selections, the Test report contains the
following data.
Header
• Global data such as software versions, instrument serial
numbers, date of creation and global test review (not
relevant)
• Signature section with operator name
Instrument Properties
Type, size, left arm, middle arm, right arm
Diagnostics Data
RS-232 host: error interrupts, repeated messages
Device Information
List which displays the following parameters for each
connected device: device name, address, firmware
version, bootware version, serial number

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Command Tool
The Command tool allows input of single line and batch file
commands for troubleshooting.
The tool, for experienced Field Service Engineers, developers,
and the Tecan production department, provides the following
functions:
• Edit and execute single commands.
• Write, edit, and execute small batch programs (command
sequences). Such batch programs can be stored in a
directory and reloaded at a later time.
• These single commands and/or batch programs allow for
performance of special test sequences not accessible
through the normal menu.
Password Protection: Functions of the Command
Tool panel are password-protected and require the use
of the appropriate password.

There is a specific command set (also known as FCS =


Firmware Command Set) for each firmware. These sets can be
used to initialize, move, etc., various devices and options.
They are documented separately and are not described in this
manual.
Select <Instrument><Command Tool>. The Command Tool
panel displays.

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Section 11 Command Tool

Batch File Execution


The first tab is the Batch File Execution page.

The Batch File Execution page contains all controls needed to


create, edit, run, store, retrieve, and delete command
sequences.
In the main field of the screen, a series of single line
commands can be strung together and executed as a batch
protocol.
The Batch File Execution page includes the following
controls.

Control Description
File Name Name of the currently loaded batch file.
Edit Window The large window is used to enter single
commands, that together compose a
command sequence. Commands can be
typed directly in the window. Each
command line must be terminated with the
Enter key.

Command Buttons
Command Description
Run All Select to execute all commands of the batch.
Stop Stops the execution of a batch.
Single Step Allows execution of the batch stepwise,
command line by command line.

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Command Description
Resume Resumes the execution of a stopped batch,
starting at the current command line.
Reset Resets the internal logic after errors.
Load... Starts the Windows Open dialog where a
batch file containing a command sequence
can be selected.
Save Saves the current contents of the Edit
window.
Save As Starts the Windows Save As dialog where
contents of the Edit window are saved with
a different name.
Read Only Enables/disables write protection of batch
files. If this checkbox is selected, batch files
can be loaded from disk but not
overwritten.
Command Text field which displays the currently
executed command.
Response Text field which displays the response of the
instrument.

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Section 11 Command Tool

Single Commands
Select the Single Commands tab. The Single Commands
page displays.
This page allows input of single command lines, a view of
instrument response, and the answer to the command.

The Single Commands page includes the following controls.

Control Description
Previous Displays previously requested single
command list: command lines. These commands can be
executed again later.
Timeout: The amount of time, in ms (milliseconds),
allowed for execution of the command.
Command: The single command line to be executed.
Answer: The response of the instrument to the single
command line.
Transmit Sends the command to the instrument and
executes the command.

NOTE: Commands entered on disk cannot be stored for


retrieval at a later time. Use the Batch File Execution
page for retrieval.
• To initiate this protocol, set the Timeout:
parameter to the desired time interval.
• Type the single command line to be executed in
the Command: field.

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• Select Transmit.
• The instrument attempts to execute the
command.
If the command was executed successfully, the appropriate
response displays in the Command: field.

If the instrument is unable to execute the command


successfully, the specific error displays in the Answer: field.

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Section 11 Information

Information
Purpose
The Information panel allows access of detailed information
regarding currently installed system devices and options
through a single panel. With this panel, screens or panels of
each individual system device or module are not opened to
obtain the required information.
No setups or tests can be performed through the Information
panel. Information panel functions are available to all users,
with no password required.

Pages
The Information panel includes the following four pages;
refer to the figures in the following paragraphs.

Page Description
Results Provides an overview of available test
results, grouped together by selectable
criteria.
Information Provides general information, parameters
and diagnostics data of installed device(s).
The displayed information depends on the
device type.
Version Provides information about the versions of
the installed system modules and panels.
Printing Configure and print the information
selected on the Results, Information or
Version pages.

Procedure
To access the Information panel: from the Main menu, select
<Instrument><Information>.

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Genesis Setup and Service Software Section 11

Results Page

Purpose
The Results page provides an overview of the stored test
results and helps to quickly locate results of a particular test.

Directory and Files


Test results are stored as follows:
Description
Directory: C:\Program Files\Tecan\Genesis Instrument
Software\Results
File name <module>_<serial_number>_<date>_<time>.an
structure: y
Example: TeVacS_1212_05Dec2001_15-47-24.any

The file name includes the following items of information


(separated by an underscore _):
• Module—Name of the module or device that was tested.
• Serial number—Number displayed depends on the test:
– Device test—the device serial number displays
– System test—the instruments serial number displays
• Date—Day/month/year when the test was performed.
• Time—Hour/minute/second when the test was concluded
(local time, 24-hour clock, i.e., 0:00:00 till 23:59:59 hours).

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Section 11 Information

Selection Criteria
The module name, serial number, date, and time are used as
selection criteria on the Results page.

1st Selection

2nd Selection

3rd Selection

4th Selection

1st Selection

2nd Selection

3rd Selection

4th Selection

Figure 11.10: Results Page - Selection Criteria

Controls
The Results page includes the following controls.

Control Description
Upper-left list Tree view with four selection levels. It serves
box as a browser for quick selection of an
individual test. The nodes (small square
boxes with + or - sign) can be expanded or
collapsed to show or hide associated
sub-selections.
Right list box Lists available results for the item selected in
the left list.
List at bottom Definition or edit of the selection order.
OK Closes the panel.

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Control Description
Cancel Closes the panel.
Print a Prints the results of the item(s) selected in
the right list.
a. Switch to the Printing page to print the results more
selectively.

Default Selection Order


Figure 11.10 illustrates the default selection order of test
results:
• 1st selection—by module (in the example above, RoMa tree
is expanded).
• 2nd selection—by serial number within 1st selection (in the
example above, there is one RoMa with serial number
1065).
• 3rd selection—by date within 2nd selection (in the example
above, tests with RoMa 1065 were run on 25 March 2003).
• 4th selection—by time within 3rd selection (the example
above illustrates the times when tests for RoMa were
concluded, 25 March 2003).

Changing the Selection Order


The selection order is given by the position of a selection
criterion in the small list below the tree view. The criterion at
the top is the first selection, the one at the bottom is the
fourth selection.

Perform the following steps to change the selection order.


1. Highlight the criterion whose position will be changed.
2. Use the arrow keys on the left side to move it up or down
the list.

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Section 11 Information

Example
There are several available options to view results of all tests
run on a specific day. For example:
• Date criterion can be moved to the top of the list and
Time criterion to the second position.
• Serial Number criterion can be moved to the bottom of
the list and Module criterion to the third position.
The screen below illustrates an example of a Results page with
results grouped by date.

Figure 11.11: Results Page - Results Grouped by Date

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Information Page

Purpose
The Information page allows selection and viewing of
detailed information regarding the entire instrument, a system
device, or an option. The type of information provided
depends on the selected item.

Figure 11.12: Information Panel

Controls
The Information page includes the following controls.

Control Description
Refresh Loads the current device and module
information, and sets the Sample Time to
the current time.
Sample Time Time when the device and module
information was last read.
Upper list Tree view of the devices or modules from
which information can be retrieved.
Lower list Displays information about the selected
item in the upper list. The number of
columns and column headings in this list
depend on the selected item.
OK Closes the panel.

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Section 11 Information

Control Description
Cancel Closes the panel.
Print a Prints the results of the item selected in the
right list.
a. Also, switch to the Printing page and print the results
more selectively.

Version Page

Purpose
This page displays the current versions of the installed system
modules and panels.

Storage of Modules and Panels


The modules and panels are stored as *.dll or *.exe files in the
following directories:
• System modules: partly in C:\Program
Files\Tecan\Genesis Instrument Software\, partly in
<windrive>\Program Files\Common Files\Tecan\
• Panels: C:\Program Files\Tecan\Genesis Instrument
Software\Modules

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Controls
The Version page includes the following controls.

Control Description
Upper list Lists the system modules and displays the
following for each module: File name,
Version, and Description.
Lower list As above for the panels.
OK Closes the panel.
Cancel Closes the panel.
Print a Prints the results of the selected item.
a. Also, switch to the Printing page and print the results
more selectively.

Printing

Purpose
This page allows selective printing of information regarding
items selected on the Results page. This provides
configuration of a report that meets laboratory requirements.
Select the necessary components for inclusion in the report,
and omit those not needed.

• The above applies only to information selected from the


Results page.

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Section 11 Information

• If items on the Information or Version page are selected,


switch to the Printing page then all available information
regarding selected items prints. In this case, only the
report header, operator name, and possible comments can
be selected or omitted.

Controls
The Printing page includes the following controls.

Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Further Depends on selections made on the Results
checkboxes page.
Multiple Data selection valid for all device data
existing selected on the other pages.
Sections
Operator Field for operator name. Refer to the
following note.
Comment Field for additional notes. Refer to the
following note.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to
setup a printer.

• If information selected on the Results page is printed,


nothing can be typed in the Operator or Comment fields!
Instead, these fields display text from the respective result
file.
• However, if information selected on the Information or
Version page should be printed, complete the Operator
and Comment fields (recommended).

Printing a Report
Perform the following steps to print a report with the Printing
page.
1. Perform one of the following:
• Go to the Results page and select the result file(s) to
print.

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Genesis Setup and Service Software Section 11

• Go to the Information page to print the respective


information.
• Go to the Version page to print the respective
information.
2. Switch to the Printing page.
3. Configure the report as necessary:
• Select the checkboxes of items to be included in the
report.
• Clear the checkboxes of items to be omitted.
4. Select Result Preview to preview the report onscreen.
5. Use Print Setup as necessary.
6. Select Print.

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Section 11 System Devices Functions

System Devices Functions

Available Arms
The following robotic arms are installed on the m2000sp
instrument:
• Liquid Handling arm (LiHa) for liquid pipetting tasks.
• Robotic Manipulator arm (RoMa) Standard for plate
handling on and underneath the platform worktable.

Device Parameters
If an arm is set up, various device parameters were determined
and written to the device. Refer to the corresponding Results
page or Test report.

Reference Positions

m2000sp Instruments
There are a total of 45 grids on the m2000sp instrument. The
reference positions for the m2000sp are set at grids 1, 7, 22, 35,
41, and 45.
NOTE: Reference positions are defined separately for
each arm.

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System Devices Menu


The System Devices menu includes the following commands.

Command Description
PosID Sets up a Positive Identification system.
Refer to Positive Identification Functions for
PosID 2, page 11-69 for PosID 2.
LiHa Sets up a Liquid Handling arm; refer to
Liquid Handling Arm (LiHa), page 11-103.
Move Test Determines if the LiHa, RoMa, and PosID
can reach the whole range (Move Test, page
11-138), and when the Random Move test is
used. Determines if the mechanics of the
PosID, RoMa, and LiHa work properly.
RoMa Sets up a Robotic Manipulator arm; refer to
Move Test, page 11-138.

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Section 11 Positive Identification Functions for PosID 2

Positive Identification Functions for PosID 2

Purpose
The Positive Identification system (PosID) uses a bar code
scanner to read bar codes on carriers, racks, and containers on
both the primary side (e.g., sample tubes) and secondary side
(e.g., microplates).

PosID Type
Positive Identification is referred to as PosID 2 in the Genesis
Instrument software package. It is also referred to as PosID 2 in
this manual. This section describes the Setup & Service
functions for the PosID 2. The m2000sp instrument is
equipped with PosID 2.

Figure 11.13: PosID

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Safety
WARNING: Class II Laser Product. Identifies a Class 2
laser product that contains an embedded low power
visible Class 2 laser diode bar code scanner. Warns
against direct viewing into the bar code laser beam or
reflections of the beam.
CAUTION: Electrostatic Discharge Hazard. Observe
precautions for handling electrostatic discharge
sensitive devices.
• Wear a wrist ground strap.
• Discharge static electricity from your body prior to
touching the instrument.

Required Tools
The PosID Service Rack, illustrated below, is required for the
setup function of the PosID 2 system module.

Guideline

Calibration lines

Figure 11.14: PosID Service Rack

Log File
Each time the test function is started, a log file is created to
which test results are written.

Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Service Log
File name: <date>_<ongoing_no>.log
Example: 00123102.log

These log files can be helpful for troubleshooting if a serious


failure occurs and it cannot be repaired yourself. Contact your
Abbott Customer Support Center specialist for assistance.

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Section 11 Positive Identification Functions for PosID 2

Movement Axes
Movements of the PosID 2 are coordinated with other system
modules, such as the LiHa and RoMa. There are three
movement axes:
Range Movement
X movement Movement of PosID 2 left-right
Y movement Movement of PosID 2 gripper
forward-backward
X' movement Vertical rotation motion of PosID 2 scanner
head
B movement Horizontal rotational motion of PosID 2
scanner head

X X

Y
X

X Arm movement: left–right Y Gripper movement: for-


X Scanner head: vertical axis of ward–backward
rotation B Scanner head: horizontal axis
of
Figure 11.15: Movement Axes

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PosID Menu for PosID 2


The PosID sub-menu for PosID 2 includes the following
commands.

Command Description
Setup Set up PosID 2.
Bar code Set bar codes.
Types
Test Check PosID 2 functionality and settings.
Movement Allow movement of PosID 2.

Mechanical Adjustment

Purpose
Mechanical accuracy must be verified before the PosID 2 can
be set up with Genesis Instrument software.
NOTE: Ensure the worktable is empty and free of
obstacles at all times.

Verifying Mechanical Accuracy


Perform the following steps to verify basic mechanical
accuracy.
1. Switch off the instrument.
2. Use a feeler gauge to check the gap between the release
finger of the PosID 2 and the workable surface as
illustrated below.

Fixing Screws

Release Finger

0.2mm

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Section 11 Positive Identification Functions for PosID 2

3. If necessary, loosen the two fixing screws and adjust the


release finger as illustrated above. Tighten the screws.
4. Place the Service Rack onto a grid position on the
worktable (refer to Figure 11.1).
5. Manually move the PosID 2 gripper into the Service Rack
as shown in Figure 11.16 on the right side.
6. Check the gap between Service Rack and gripper pin.
Adjust to approximately 0.5 mm as illustrated in
Mechanical Adjustment, page 11-73 on the left side.
7. Switch on the instrument and initialize it with <System>
<Initialize Instrument>.

Carrier
Adjusting Slot

Carrier

0.5 mm

Gap between Gripper Pin and Carrier

Figure 11.16:Mechanical Adjustment

Change Position
The change position is the transition position in which the
scanner head goes from moving around its vertical B'-axis to
moving around its horizontal X'-axis, or vice versa. Figure
11.11.17 on page 11-74 and Figure 11.11.18 on page 11-74
illustrate the change position.

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Genesis Setup and Service Software Section 11

(1) Carrier bar code reading position.

(2) Turning around B-axis to the change


position (refer to Figure 11.18).

(3) Turning around the X-axis to the rack


reading position.

Figure 11.17: Possible Scanner Head Positions and Movements

Incorrect change position:


• Note the gap between the scanner
head edge and X-axis.

Correct change position:


• In the correct position, there is almost
no gap between the scanner head
edges and X-/B-axes.

Incorrect change position:


• Note the gaps between the scanner
head edges and X-/B-axes.

Figure 11.18: Change Position of Scanner Head

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Section 11 Positive Identification Functions for PosID 2

X-, Y-, and B-axes Setup


During the Setup procedure (Setup Procedure, page
11-76)—when settings of the logical positions are configured
and stored in EEPROM— values for the offset and scale factor
are newly calculated according to the following tables.

Defaulta Basicb
X-offset no default 780
Scale Factor no default 10000

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Logical
Default a Basic b
Position
Y-offset no default 15
Load 1 2220 2220
Remove 2 3170 3170
Max Read 3 0 0
Connect 4 3170 3170
Push Connect 5 3170 3170
Carrier Present 6 3000 3010
Check
Init Position 7 2850 2850
Test Rack 8 3100 3099

Logical
Default a Basic b
Position
B-offset 80 80
Carrier Read 1 0 0
Position
Horizontal Read 2 2610 2650
Position (MTP)
Vertical Read 3 1350 1350
Position
(Sample/Reagent)
Collision Avoidance 4 0 0
Position
Turn Position 5 1410 1410
a. Default values set in the Setup Procedure by software (refer
to Figure 11.11.19 on page 11-78).
b. Basic values, used when PosID 2 is new (factory set)

These values are hardcoded in the software. Logical positions


are indicated in steps.

Setup Procedure
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

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Section 11 Positive Identification Functions for PosID 2

Before Starting Setup


Ensure the following conditions exist:
• All installed arms (LiHa and RoMa) are set up correctly.
• The correct worktable size is set. Start <Instrument>
<Basic Setup><Worktable Size> to check/correct the
worktable size.
• The X-drive properties are set correctly. Start
<Instrument><Basic Setup><Set X-drive Properties> to
check/correct them.
• Mechanical adjustments (Mechanical Adjustment, page
11-72) were performed.
• The worktable is empty and free of obstacles at all times.
NOTE: Verify the PosID 2 was successfully set up and
configured, following this setup procedure. Inaccurate
settings can result in misreading, i.e., mistaking one
tube for another.
NOTE: When selecting the movement buttons within
the Setup function, do not click the mouse too fast. The
software could fail, then the Setup & Service software
and PosID 2 Setup function must be restarted.
Settings which provide meaningful results are permanently
saved in EEPROM.

Purpose
This procedure is used to set up the PosID 2 with the aid of the
Setup & Service software.

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Starting Setup
Perform the following steps to set up the PosID 2.
1. Start with <System Devices><PosID><Setup>. The PosID
2 Adjustment dialog (Figure 11.19) displays.

Adjustment Dialog

Figure 11.19: PosID 2 Adjustment Dialog

Adjustment Procedure
1. Select Set to perform the following:
• Reset the default B-offset and the logical positions of
the bar code scanner head for the B-axis. The PosID 2
moves through all five logical positions.
• Reset the logical positions of the gripper for the Y-axis.
The gripper moves through all eight possible logical
axes.
• Store results in EEPROM.
2. Select Run. The PosID 2 runs in all axes to the end
positions to determine its range.

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Section 11 Positive Identification Functions for PosID 2

• Performs autorange function and calculates number


of grids.
• Store results in EEPROM.
3. Select Start to start the setup. A prompt displays to insert
the test rack in the defined position (in the example,
Figure 11.11.20 on page 11-79, the defined position is #1).
4. Slide the Service Rack into the recommended position
(Left Position). Do not push it to its stop position. Verify
it is approximately 1 cm in front of the stop position.

Figure 11.20:Insert Test Rack Message

5. Confirm with OK. The Move PosID: Move Gripper to


position 1 dialog displays.

Figure 11.21:Move PosID: Move Gripper to Position 1 Dialog

The Move PosID dialog includes the following controls.

Description
X List to select the movement step size,
in mm, in X-direction.
Left Moves the PosID 2 arm from right to left in
X-direction.

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Description
Right Moves the PosID 2 arm from left to right in
X-direction.
Y List to select the movement step size,
in mm, in Y-direction.
Rear Moves the gripper from front to rear.
Front Moves the gripper from rear to front.
OK Confirms the adjustment.
onscreen Aborts the procedure without saving any
changes.

6. Carefully push the Service Rack toward the rear of the


instrument to its stop position.
7. Check the position of the gripper pin to the adjusting slot
of the Service Rack. The pin must fit exactly in the slot as
illustrated in Figure 11.16.
8. If the position is incorrect, align it with the movement
buttons.
9. When the gripper pin fits in the slot, confirm the
adjustment with OK. The message Please insert Test Rack
in Position x displays.
10. Slide the Service Rack into the recommended position
(Right Position). Do not push it to its stop position. Verify
it is approximately 1 cm in front of the stop position.
11. Confirm with OK. The Move PosID: Move Gripper to
position x dialog (similar to Figure 11.21) displays.
12. Carefully push the Service Rack toward the rear of the
instrument to its stop position.
13. Check the position of the gripper pin to the adjusting slot
of the Service Rack. The pin must fit exactly in the slot as
shown in Figure 11.16.
14. If the position is incorrect, align with the movement
buttons.
15. When the gripper pin fits into the slot, confirm the
adjustment with OK. The message Please insert Test Rack
in Position x displays.
16. Slide the Service Rack into the recommended position
(Rack Position) into its stop position.

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Section 11 Positive Identification Functions for PosID 2

17. Confirm with OK. The mechanical adjustment starts with


the Move PosID: Mechanically adjust beam angle
vertical dialog (Figure 11.22).

Scanner Head Mechanical Adjustment


The following procedure describes mechanical adjustment of
the scanner head.
During the mechanical adjustment, all movements using the
command buttons are not stored. The software adjustment
follows later starting with step 1.
Figure 11.22: Move PosID: Mech. Adjust Beam Angle
Vertical Dialog

1. The laser beam must be parallel to the vertical guideline


on the Service Rack (refer to Figure 11.23).

Carrier ID Read Position

Guideline
Laser Beam

Correct Incorrect

Figure 11.23: Vertical Laser Beam Adjustment

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2. If the beam is not parallel, loosen one of the two fixing


screws for the vertical adjustment with a 2.5 mm Allen
key, and adjust the scanner head accordingly. Tighten
both screws after the adjustment is complete.
Fixing Screws for Horizontal Adjustment
(Step x)

Fixing Screws for Horizontal and


Vertical Adjustment (Step x)

Fixing Screw (Vertical Adjustment)


(Step x)

Fixing Screw (Vertical Adjustment)


(Step x)

Figure 11.24:Scanner Head Adjustment Screws

3. Confirm with OK. The gripper finger moves the Service


Rack to its center position. The Move PosID:
Mechanically adjust head vertical dialog displays.

Figure 11.25:Move PosID: Mech. Adjust Head Vertical


Dialog

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Section 11 Positive Identification Functions for PosID 2

The laser beam must be parallel to the vertical calibration line


on the Service Rack.
Service Rack

Horizontal Calibration
Line

Vertical Calibration Line

Laser Beam

Correct Incorrect
Figure 11.26: Vertical/Horizontal Scanner Head Adjustment

4. If the beam is not parallel, loosen one of the two fixing


screws for horizontal and vertical adjustment with an
Allen key, then adjust the scanner head as necessary.
Tighten both screws after the adjustment is complete.
5. Confirm with OK. The Move PosID: Mechanically adjust
beam height dialog displays.

Figure 11.27:Move PosID: Mech. Adjust Beam Height


Dialog

The Move PosID dialog includes the following controls.

Control Description
Beam Angle List to select the rotation step size, in
degrees, in B-direction.
CCW Rotates the scanner head
counterclockwise.

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Control Description
CW Rotates the scanner head clockwise.
OK Confirms the adjustment.
Cancel Aborts the procedure without saving any
changes.

The laser beam must be parallel and flush to the


horizontal calibration line on the Service Rack (similar to
Figure 11.26).
6. Perform the parallel adjustment with the movement
buttons.
NOTE: Because of the play (hysteresis), movement
should be done from clockwise direction only.
Therefore, first move the scanner head with CCW
and back to the correct position to the calibration
line with CW.
7. If the beam is not in line with the horizontal calibration
line, insert an Allen key through the access hole and move
the scanner head until the beam is flush with the
horizontal calibration line.
8. Confirm with OK. The mechanical adjustment is executed
and the position of the bar code reader is stored. The Move
PosID: Adjust carrier reading position dialog displays.

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Section 11 Positive Identification Functions for PosID 2

Scanner Head Software Adjustment


The laser beam must be parallel (adjusted in step 3 of the
previous procedure) and flush with the vertical guideline on
the Service Rack. Refer to Figure 11.23.
1. Perform the adjustment using the rotation buttons.

Figure 11.28:Move PosID: Adjust Carrier Reading


Position Dialog

2. Confirm with OK. The Move PosID: Adjust change


position dialog displays.

Figure 11.29:Move PosID: Adjust Change Position


Dialog

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The change position is the transition position in which


the scanner head changes from moving around its
vertical B-axis to moving around its horizontal X-axis, or
vice versa. Refer to Mechanical Adjustment, page 11-72
for details.
NOTE: Because mechanical movement always has
play (hysteresis), it is important to start the
adjusting procedure from the same direction.
NOTE: Always rotate the scanner head with CCW
back to the correct position to avoid any
mechanical problems (collisions).
3. With the Beam Angle list, set the step size to 1.0.
4. Rotate the scanner head counterclockwise with CCW until
it rotates, to ensure it is not already in the change position.
5. Rotate the scanner head clockwise with CW until it stops
rotating in the vertical B'-axis.
6. Count the number of steps necessary until the scanner
head starts to rotate into the horizontal X'-axis.
7. Go back counterclockwise, half the number of steps
counted plus two additional steps.
NOTE: These two additional steps are necessary to
compensate for space in the mechanical system.
8. Repeat these two steps but clockwise, to arrive exactly at
the change position.
9. If the change position is still incorrect, the adjustment
must be performed again (steps 34 to 39) with a step size
of 0.1 mm.
10. Confirm with OK.
11. The PosID starts with the No Tube Sensor test. The gripper
finger slowly moves the Service Rack backward in the
Y-direction until the No Tube sensor passes all three slots
in the Service Rack.
12. If the No Tube Sensor test passed, the following four
checkboxes are selected (聺) in the PosID 2 Adjustment
dialog.
• Slot begin
• Slot end

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• Check sensor upper limit


• Check sensor lower limit
13. If the No Tube Sensor test failed, the No Tube sensor is
damaged and must be replaced.
14. The setup procedure continues. The gripper finger moves
the Service Rack to its center position and the Move
PosID: Adjust tube reading position dialog displays.

Figure 11.30:Move PosID: Adjust Tube Reading


Position Dialog

The laser beam must be in line with the vertical


calibration line of the Service Rack.
15. If the laser beam is out of line, adjust the scanner head
with the rotation buttons. Confirm with OK. The tests for
Adjust scan line start and Adjust scan line stop (refer to
Figure 11.19) are performed automatically.

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16. The PosID 2 adjustment was completed successfully and


the message Adjust passed displays.

Figure 11.31:Adjust Passed Message

17. After passing the Setup procedure successfully, the


PosID 2 Adjustment dialog (Figure 11.19) displays again.
Except for the Adjust failed checkbox, checkboxes are
checked (聺) to indicate all appropriate tests were
completed.
If the setup procedure failed, the Adjust failed checkbox
is checked (聺), and the setup procedure must be
repeated.

Bar Code Types


For the m2000sp instrument, bar code setting is accomplished
within the m2000sp software.
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Introduction
Default bar code settings are reloaded with each cycling of the
power. The PosID 2 is capable of simultaneously reading six
different bar codes in a run, although m2000sp only supports
five bar code types.

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Section 11 Positive Identification Functions for PosID 2

Twenty-two different bar codes are stored in the Setup &


Service software.
Table 11.32: Bar codes Stored by Setup & Service Software
Bar code Check Start-Stop
Length Digit Mode Character
Code 2 of 5 enabled enabled -
interleaved
Code 39 Standard enabled enabled enabled
Code 39 Full ASCII enabled enabled enabled
Code 32 fixed 9 - -
C.I.P. enabled - -
Codabar enabled enabled enabled
Code 128 enabled - -
EAN 128 enabled - -
Code 93 Standard enabled - -
Code 93 Full ASCII enabled - -
EAN 13 fixed 13 - -
EAN 8 fixed 8 - -
UPC A fixed 12 - -
UPC E fixed 8 - -
EAN 13 add on 2 fixed 15 - -
EAN 8 add on 2 fixed 10 - -
UPC A add on 2 fixed 14 - -
UPC E add on 2 fixed 10 - -
EAN 13 add on 5 fixed 18 - -
EAN 8 add on 5 fixed 13 - -
UPC A add on 5 fixed 17 - -
UPC E add on 5 fixed 13 - -

NOTE: Bar code settings can be loaded from three


sources: from the PosID, *.ini file, and Setup & Service
software (default values).
However, only the *.ini file can be stored in a
non-volatile memory. All other settings default to the
original configuration after switching off the
instrument.

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The following bar code types are supported by the m2000sp


software and will override any settings mode in S&S.

Start Stop
Bar code Check Digit
Bar code Type Character Active
Length Mode
Transmission
Code 2/5 2,4,6,8,....,20* ON with or No setting Yes
Interleaved without
transmission or
OFF
Code 39 1-20 ON with or No setting Yes
Standard without
transmission or
OFF
CODABAR flexible 1-20 ON with or ON with or Yes
without without
transmission or transmission or
OFF OFF
Code 128 flexible No setting No setting Yes
Code 93 flexible 1-20 No setting No setting Yes

Example
The following schematic shows a bar code of type Code 39
Standard. Information about bar codes can be found in
literature supplied by the bar code or test kit manufacturers,
respectively.
*1234567890123456N*

Stop character
Check digit
bar code
Start character

Figure 11.33: Key to Bar code “Code 39 Standard”

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Section 11 Positive Identification Functions for PosID 2

Bar code Setting Procedure


Bar code configurations are set in the m2000sp software. Setup
and service software should not be used for this function.
NOTE: For FSE Troubleshooting. When power to the
instrument is turned off, the bar code settings are not
stored permanently by the PosID. To troubleshoot bar
code reading issues, the '*.ini file' established during
instrument installation must be reloaded then sent to
the PosID. Go to <System Devices><PosID><Bar code
Types> and select Reload from Ini File. Verify the bar
code settings reflect what is used in the laboratory.
Select Send to PosID to send the settings to the PosID.
Functionality of the bar code scanner can now be tested
based on bar codes in use in the laboratory.

Test

Purpose
The Test function verifies proper functioning of the bar code
scanner.
To run the Test function, at least two carriers (e.g., tube rack,
microplate carrier, etc.) must be available. The carriers must
have correctly placed bar code labels.

Test Sequence
During the test, the carrier and container bar codes are read in
the following sequence:
• Carrier ID, and if desired, user ID bar code of all carriers
(Reference Read).
• Bar codes on every container on every carrier (Reference
Read).
• Steps 1 and 2 for the defined Number of cycles (Actual
Read).

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Test Procedure
Perform the following steps to check functionality of the
PosID.
1. Start the function with <System Devices><PosID><Test>.
The PosID 2 Test Configuration dialog displays.

Figure 11.34:PosID 2 Test Configuration Dialog

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The PosID 2 Test Configuration dialog includes the


following controls.

Control Description
Number of cycles Field for the number of Actual Reads
to be performed.
To pass the test, at least 5 cycles
must be performed.
Grid Positions Field to define the range from…to
where the PosID test will run.
Carries Codes Checkboxes to specify which bar
codes are read.
Read Carrier Carriers and containers.
Only Carrier Only carriers (no containers, e.g.,
ID tube, microplate, and trough bar
codes).
Read User ID User identifications.
Operator/Logfile Fields for operator name, PosID SN,
and log file.
Operator Field for operator name. An entry is
required.
Serial number Field for PosID serial number.
Logfile Field to define the log file directory.
An entry is required. The default
path is C:\Program
Files\Tecan\Genesis Instrument
Software\Service Log.
The log file name itself is based on
the date and an ongoing number,
e.g., 00123102.log, and contains a
summary of the test results.
Logfile Starts the standard Windows dialog
Directory to browse and find a directory for
the log file.
Run Opens an empty PosID 2 Test Results
window to start the test procedure.
Close Finishes the function and saves the defined
values.
Cancel Aborts the function without saving any
changes.

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Control Description
Save Starts the standard Windows Save As dialog
to save the data in an appropriate *.ini file in
the C:\Program Files\Tecan\Genesis
Instrument Software\Service directory.
Print Prints the test results stored in the defined
log file. It is enabled only if a test was run.
More Opens the PosID 2 Test - Advanced
Configuration dialog for advanced
configuration; refer to Advanced
Configuration on page 11–98. For FSEs only
and requires the appropriate password.

Password Protection: This procedure is exclusively for


FSEs and requires use of the appropriate password.

2. Set the appropriate start parameters and enter the operator


name in the Operator field.
3. Place the carriers for the test on the worktable. Ensure the
bar code labels are placed correctly. The carriers must be
placed within the grid positions as defined under Grid
Positions.
4. If definition of an advanced configuration is desired,
continue with More.
5. Start the test with Run. An empty PosID 2 Test Results
window (Figure 11.35) displays.
6. Begin the test procedure with Start. The instrument
initializes and the PosID starts checking the carriers and
containers.

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Test Results
During the test, values in the PosID 2 Test Results window are
continuously updated.

Figure 11.35: PosID 2 Test Results Window for Container


Reads

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Data on Results Window


The PosID 2 Test Results window includes the following data.

Description
Heading Number of reads done/defined and worktable
Line position, for which results display in the table.
The position can be switched with the scroll
bar.
NT Reading result of the No Tube sensor during
the Reference Read.
T Tube found.
N No tube found.
Reference Bar codes read in the Reference Read.
Read *** No reading possible, but tube is placed
correctly.
$$$ No tube found in this position.
NT Reading result of the No Tube Sensor during
the Actual Reads.
Actual Read Bar codes read in the Actual Reads.
NT No Tube: no tube found in the read attempt.
NR No Read: no bar code(s) were read, but tubes
were placed correctly.
WR Wrong Read: bar code(s) read do not match bar
code(s) read in the Reference Read.
Total Errors Total number of errors that occurred during a
test run. For the pass/fail criteria, see below.

Controls on Results Window


The PosID 2 Test Results window includes the following
controls.

Field Description
Run
Start Starts the PosID test procedure.
Stop Stops a running test procedure; the current
Actual Read is completed before
interrupting the test.
Close Finishes the function.
Reference Read

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Field Description
Save Saves the Reference Read data in the
PId2000.ini file in the C:\Program
Files\Tecan\Genesis Instrument
Software\Service directory.
Load Loads the Reference Read data from the
PId2000.ini file. No new Reference Reads
are done.
Show Carrier Option to select the carrier reads data view.
Reads Only the carrier read data for all defined
worktable positions are displayed in one
table.
Show Option to select the carrier read and
Container container read data view (refer to Figure
Reads 11.11.35 on page 11-95).

Completion of Test
When the test is completed, the message PosID 2 Test passed
or PosID 2 Test failed displays.
1. Confirm with OK. The list of values in the PosID 2 Test
Results window is complete.
2. After finishing the test, select Close to close the window.
The PosID 2 Test Configuration dialog is active again.
3. Print the test results with Print and/or finish the function
with Close.

Pass/Fail Criteria
The test passed after meeting the following requirements (refer
to Figure 11.11.35 on page 11-95, Total Errors section).
No Tube: 0 errors
No Read: not more than 5 errors
Wrong Read: 0 errors
At least 5 Actual Reads (Number of cycles) must be
performed.

If the test failed, check for correct positioning of the bar code
labels and bar code types (Bar Code Types, page 11-88), and/or
setup of the PosID (Positive Identification Functions for PosID
2, page 11-69).

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If the test fails again, contact your local Abbott customer


support representative.

Advanced Configuration
This function allows FSEs to define or change additional
parameters.
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Perform the following step to enter advanced configuration


data.
Select More on the PosID 2 Test Configuration dialog. The
PosID 2 Test - Advanced Configuration dialog displays.

Figure 11.36: Advanced Configuration

Controls
The PosID 2 Test - Advanced Configuration dialog includes
the following controls.

Control Description
Carrier List to select a specific carrier to change its
Parameter parameters: Reading Speed and Decode
Safety can be set individually for each
carrier. The Reference Read Speed Factor
remains the same for all carriers.
Reading Field for movement speed, in 1/10 mm/s,
Speed in X- and Y-direction during the read
procedure.

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Control Description
Decode Field to set the number of times the
Safety scanner reads the bar codes. This sets the
number of matching decodes required
until a bar code read is accepted as
successful. Range: 1 (low) to 10 (high). The
default setting is 2.
The ratio of Decode Safety and Reading
Speed must be either: high Decode Safety
and low Reading Speed; or low Decode
Safety and high Reading Speed. If not, an
error message displays.
Scan Scanner head-specific parameters; not
Window Min editable.
Scan Scanner head-specific parameters; not
Window editable.
Max
Set default Sets Reading Speed and Decode Safety to
the default values for the selected Carrier
Parameter.
Reference Read Option buttons to reduce the Reading
Speed Factor Speed during the Reference Read to the
selected fraction for a safer read of the bar
codes. For the remaining Actual Reads, the
read is done with the defined Reading
Speed.
Global Checkboxes to eliminate the safety
Optimization movements in B- and Y-directions for a
faster run.
Options
Send bar Checkbox to send the bar code setting
code settings defined with <System Devices><PosID>
on start <Bar code Types> (Bar Code Types, page
11-88) to the PosID at the start of the test
run.

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Control Description
Use If this checkbox is selected, the No Tube
NT-Sensor (NT) sensor is switched off during the read
alternate procedure (i.e., when the gripper finger
moves the rack backward). The No Tube
sensor is activated when the gripper finger
returns the rack to its position. This
procedure reduces the risk of read failures,
because the No Tube sensor and the
scanner usually work concurrently.
Set bar Opens the Bar code Settings dialog to set
codes the bar codes (refer to Bar Code Types, page
11-88).
OK Finishes the advanced configuration and
saves the defined values.
Cancel Aborts the advanced configuration without
saving any changes.

4. Enter the advanced configuration data.


5. Confirm the entries with OK. The PosID 2 Test
Configuration dialog is active again (refer to Test, page
11-91).

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Section 11 Positive Identification Functions for PosID 2

Movement

Purpose
The Movement function allows movement of the PosID
according to the entries.

Moving the PosID 2


Perform the following steps to move the PosID.
1. Start with <System Devices><PosID><Movement>. The
Move PosID window and PosID Test Movements dialog
open simultaneously (Figure 11.37).

Figure 11.37:Move PosID Window and PosID Test


Movements Dialog

The Move PosID window includes the following controls.

Control Description
Move X List to select the movement step size,
in mm, in the X-direction.
Left Moves the PosID arm from right to left
in the X-direction.
Right Moves the PosID arm from left to right
in the X-direction.
Move Y List to select the movement step size,
in mm, in the Y-direction.
Rear Moves the PosID gripper from front to
rear.
Front Moves the PosID gripper from rear to
front.

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Control Description
Move Beam List to select the rotation step size, in
Angle degrees, in the B-direction.
CCW Rotates the scanner head
counterclockwise.
CW Rotates the scanner head clockwise.

2. Use the command buttons to move the PosID as needed.


3. Finish with OK.

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Section 11 Liquid Handling Arm (LiHa)

Liquid Handling Arm (LiHa)


WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of the instrument,
which are not completely decontaminated. Wear
appropriate personal protective equipment such as
gloves, lab coat, and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. The exposure to hazardous chemicals is
minimized by following instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instruments safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

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LiHa
The m2000sp instrument is equipped with one 8-tip Liquid
Handling arm (LiHa). The LiHa is used for pipetting actions in
different volume ranges. Volumes depend on the tip types
used and features of the liquid system.

Liquid Handling
Arm

Figure 11.38: Liquid Handling Arm (LiHa)

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Section 11 Liquid Handling Arm (LiHa)

Movement Axes
LiHa movements are coordinated with other system devices
such as the RoMa. There are four different movement axes.

Ys

Y Z

X Arm movement: left–right Ys Horizontal spacing


Y Tip movement forward–back- Z Tip movement up–down
ward
Figure 11.39: Movement Axes

Required Tools
Tools required for performing the adjustments and tests
described in this section depend on the installed tips:
• Reference tip—The reference tip is necessary for checking
the Z-range and the reference positions in the X- and
Y-axis.
IMPORTANT: There are now two LiHa reference tip
! types available. Either can be used for S&S functions;
however, procedures for mounting each type differ
significantly. Refer to Reference Tips on page 4–54 of
Section 4.

Before Starting
During some of the following setup and test procedures, the
LiHa moves to different positions on the worktable. Ensure no
objects are installed that could be in the way during these
procedures.

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Verify the instrument is set up correctly with <Instrument>


<Basic Setup>.

Panel Description
The LiHa panel provides several procedures to be executed.
Generally, the following steps are performed.
1. Start the LiHa panel  LiHa Panel, page 11-108.
2. Select the setup/test procedures to be performed and
modify the corresponding settings.
There are two ways to proceed:
• Select ALL Setup and Test checkboxes (聺) at the same
time and enter the necessary data by selecting the
corresponding tabs. Select Start then all setup/test
procedures are performed in one run.
• Select one or more Setup or Test checkboxes and
define the necessary data as described. Select Start to
perform the currently selected setup procedures.
• If the second method is selected, start with the
topmost setup procedure and work downward. Use
the second method to repeat an individual setup.
Before starting any setup procedure, the Operator field
on the Printing page should be completed.
3. Start the desired procedure with Start.
4. After performing any test, refer to Information Page, page
11-133.
5. Print the test results. Refer to Printing, page 11-135.
6. Exit the panel with Close.

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Section 11 Liquid Handling Arm (LiHa)

Procedures and User Permission

Refer to
Operator FSE
Section
Set Default Parameters X Set Default
Parameters,
page 11-118
Determine Range X Determine
Range, page
11-118
Calibrate Lower DiTi Eject X Calibrate the
Lower DiTi
Eject
Protocol,
page 11-125
Determine Reference X Determine
Positions Reference
Positions,
page 11-121
Determine Spacinga Determine
Spacing, page
11-123
Individual Zb X X Individual Z
Alignment,
page 11-124
Arm Position Accuracya
Tip Adapter X X
Information X X Information
Page, page
11-133
Printing X X Printing,
page 11-135
Test Report X X Test Report,
page 11-136
a. This function is for production only and is not accessible to FSEs.
b) Not used with the m2000sp.

Directory, Files
The LiHa function can create the following files. Refer to
Liquid Handling Arm (LiHa), page 11-103 for details.

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• Result Files

Description
Saved in C:\Program Files\Tecan\Genesis Instrument
directory: Software\Results
File name: LiHa_<serial_number>_<date>_<time>.any

LiHa Panel

Pages
The LiHa panel is subdivided into the following pages.

Page Description
Contents General overview, procedure/test selection.
Channel Setup/check of tips (length/types) and
syringe sizes.
Setup Definition of tips and other parameters for
the test.
Test For Tip Adapter and Arm Position Accuracy
Configuration tests; Arm Accuracy test is for production
only.

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Section 11 Liquid Handling Arm (LiHa)

Page Description
Information Device parameters and diagnostic data; refer
to Information Page, page 11-133.
Printing Print selection for the Test report; refer to
Printing, page 11-135.
Move LiHa Movement tool.
Dilutor Tool used to check dilutors and tubings.

Controls
The Contents page includes the following controls.

Control Description
Available Devices Shows the connected LiHa.
Set Default For setting default values.
Parameters
Determine Range For determining Y and Z range; refer
to Determine Range, page 11-118.
Determine Reference For determining/checking reference
Positions positions; Determine Reference
Positions, page 11-121.
Determine Spacing For calculating spacing. Not used on
the m2000sp.
Arm Position Production function, not accessible
Accuracy to operators and FSEs.
Tip Adapter Tip adapter test.
Individual Z For determining/checking Z-range
of each individual tip. Not used on
the m2000sp.
384 Well Tip Adjust Not used on the m2000sp.
384 Well Tip Verify Not used on the m2000sp.

It also contains the standard command buttons; refer to LiHa


Panel, page 11-108.

Using the LiHa Panel


Perform the following steps to set up the LiHa.

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1. Start panel with <System Devices><LiHa>. The Contents


page displays.
2. Select the LiHa in the Available Devices section and set
the valid Serial Number of the LiHa (refer to NOTE below).
3. The LiHa’s serial number is not stored, and is lost when
the instrument Setup & Service software is exited. The
serial number must be entered each time the LiHa panel is
started.
4. Use the serial number of the instruments.
5. Before continuing, select the Channel page and verify
that tip types and lengths, syringe sizes, etc. are set
correctly. Refer to Channel Page, page 11-110 for details.
6. Select the setup and test procedures to be performed and
define necessary data as described in the appropriate
section. All setup/test procedures can be selected, or one or
more for the desired run. Refer to Panel Description, page
11-106 for details.
7. Before starting any setup/test procedure, switch to the
Printing page (LiHa Panel - Printing Page, page 11-135) to
complete the Operator and Comment fields.
8. Start the desired procedure with Start.
9. Switch to the Information page to view diagnostics data
(Information Page, page 11-133) or switch to the Printing
page to preview the Test report (Printing, page 11-135).
10. Finish with Close.

Channel Page

Purpose
The Channel page allows the operator to check and define the
following:
• Tip configuration
• Lengths of user-defined tips (FSE password required)
• Syringe size (FSE password required)

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Section 11 Liquid Handling Arm (LiHa)

• Low Volume option (not used with the m2000sp


instrument)

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Controls
The Channel page includes the following controls.

Control Description
Ch Channel number.
Type Type of the installed tip.
Length Length difference, in mm, of the tip relative
to the reference tip. A positive value
indicates the tip is longer than the reference
tip, a negative value that it is shorter.
DiTi Can be checked if the respective tip is a
DiTi.
Pinch Checkboxes for selecting LowVol tips. Not
used with the m2000sp instrument.
Active Indicates the tip is an active tip; the
checkbox cannot be set and the system
automatically determines if the tip is active.
Not used with the m2000sp instrument.
Syringe Syringe volume in µL (microliter).
Group Checkboxes to set identical parameters for
multiple channels. In this case, channels
1 to 8 have identical parameters. Set the
parameters for channel 1 and select the
Group boxes. Parameters of subsequent
channels (if their Group boxes are not
selected) are set to equal those of channel 1.

Mixed Tip Configurations


The m2000sp instrument uses 200 µL and 1000 µL filtered
Eppendorf tips. Any combination of tip types is possible.
However, grouping together tips of the same type is
recommended
Tips of the same type can be selected together for a test. If tip
types differ, the test can be run once per tip type, testing all
the tips of one type each time.

Setting Tip Configuration


Perform the following steps to configure tips that are
physically installed.

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Section 11 Liquid Handling Arm (LiHa)

1. Start the LiHa panel with <System Devices><LiHa> and


switch to the Channel page.
2. Configure the tips according to the installed types.
3. Confirm the entries:
• Select OK to store the configuration then exit the
LiHa panel.
• Select Apply to save the entries then perform further
setups/tests within the LiHa panel.

Setting Tip Length


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

The tip length can be set on the Channel page. Tip lengths do
not require edits if the tips are supplied by Tecan.
Perform the following steps to set the tip length.
1. If not already done, start the LiHa panel and select the
Channel page.
2. Check the length of the tips in use and enter the difference
between the reference tip in the appropriate field.
3. Confirm the entries:
• Select OK to store the configuration then exit the
LiHa panel.
• Select Apply to save the entries then perform further
setup/test procedures within the LiHa panel.

Setting Syringe Size


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

The Channel page contains eight fields which allow setting of


syringe volumes for each individual dilutor. The m2000sp
instrument uses 1.0 mL syringes.
CAUTION: Ensure the syringe sizes are set properly.
Incorrect syringe volumes could lead to incorrect
results.
Perform the following steps to set the syringe sizes.

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1. If not already done, start the LiHa panel and select the
Channel page.
2. Check the volumes of syringes installed on the
instrument. To set the correct values, use the up or down
arrows of the corresponding spin boxes.
3. Confirm the entries:
• Select OK to store the configuration then leave the
LiHa panel.
• Select Apply to save the entries then perform further
setup/test procedures within the LiHa panel.

Move LiHa Page

Purpose
The Move LiHa page allows movement of the whole LiHa or
parts of it in the appropriate axes: X, Y, XS, Z1 to Z8. Refer to
Move LiHa Page, page 11-114 and LiHa, page 11-104for details.

Controls
The Move LiHa page includes the following controls.

Control Description
All Tips/Tip1/ Checkboxes to select the tips to be moved in
Tip8 the Z-direction.

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Section 11 Liquid Handling Arm (LiHa)

Control Description
Single step, in 1/10 mm, movement
buttons.
Ten steps, in 1 mm, movement buttons.

Continuous movement buttons when


button is kept pressed.
Buttons to increase or decrease spacing to
logical positions (9/18/27/36 mm).
Free Moves the RoMa and PosID to their extreme
positions.
X, Y, YS Text boxes that indicate the X/Y-position of
the frontmost tip with respect to reference
position #1, and spacing.
Text boxes left Show the Z-position of each individual tip
of Tips (distance from worktable).
checkboxes

• Arrows on the movement buttons indicate the direction in


which the respective parts will move.
• If the mouse pointer is placed over a movement button,
text displays to explain the button’s function (“tool tip”).

Keyboard Control
The keyboard can also be used for movements in the various
axes. Refer to View and Tools Functions, page 11-34 for details.

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Setup Page

Purpose
The Setup page provides controls for setting the parameters for
the various setup procedures.

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Section 11 Liquid Handling Arm (LiHa)

Controls
The Setup page includes the following controls.

Control Description
Determine Frame with controls to specify the
Reference conditions for determining the reference
Positions positions.
automatically Option button for automatic determination.
manually Option button for manual determination.
Verify with Checkbox selected if the “normal” tip is
mounted tip used for manual verification instead of a
reference tip. Not used with the m2000sp
instrument.
Reference Frame to set the reference positions.
Positions
Grid Spin boxes to set the left and right reference
grid positions.
Use for Y Option buttons that specify if the reference
positions in the Y-axis should be determined
at the left or right reference position.
4/8 Reference Checkbox selected if a references tip is
Tips Available mounted at each tip adapter.
Use Y-backlash Checkbox must be selected for the m2000sp
instrument.
Input box cannot be set by FSE.
ABD arm For production only, cannot be set by FSE.
Not used with the m2000sp instrument.
Initialize Position Spin box used if a Te-MO is installed on the
X instrument. Defines the position relative to
grid 1 at which the arm is initialized. The
position must be chosen to eliminate
possible collisions with the Te-MO. The
position can be set with an accuracy of
0.1 [mm]. Not used with the m2000sp.
Table below Shows current speed and acceleration in X-,
Reference Y-, and Z-axes.
Positions

NOTE: Ensure all parameters are set correctly. Refer to


descriptions of the respective setup procedures for
additional information about the entries.

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Set Default Parameters


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

NOTE: Do not start this function unnecessarily. After


performing this function, it is necessary to readjust all
ranges, reference positions, offsets, and spacings.

Purpose
This function resets drive properties to the default values. It
should only be used:
• When a new instrument is set up the first time.
• After certain repairs from which maladjustment could
result, e.g., removal and reinstallation of the worktable,
replacement of entire LiHa, etc.

Procedure
Perform the following steps to set the default parameters.
1. On the Contents page, select the Set Default Parameters
checkbox (聺) then select Start.
2. When finished, all ranges, reference positions and
spacings must be adjusted with the respective functions.

Determine Range
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Determine Range procedure performs the following:
• Moves all tips back and forth in the Y-direction to
determine the available Y-range.
• Moves the tips one by one in the Z-direction to determine
available Z-ranges. A reference tip must be mounted at the
tip position whose Z-range is being determined.
• Determined values are written to the LiHa.

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Section 11 Liquid Handling Arm (LiHa)

Procedure
Depending on the quantity of reference tips available, the
following instructions can vary slightly.
• If there is only one reference tip available, the software
prompts when to mount it to a particular tip position.
• If there are enough reference tips available for all tip
positions, a reference tip can be mounted to each position
before starting the test.

NOTE: There are two reference tip types available.


Refer to Reference Tips on page 4–54 of Section 4.
Perform the following steps to determine the range.
1. On the Contents page select the Determine Range
checkbox (聺) and switch to the Setup page (Figure 11.40).
2. Select the 4/8 Reference Tips Available checkbox (聺) if
there are enough reference tips available for all tip
positions. If not, clear the checkbox (□).
The Use backlash checkbox is selected by default for
m2000sp Instruments. Do not clear it!

Figure 11.40:LiHa Panel - Setup Page - Reference Page

3. Set the Left and Right grid positions in the corresponding


Grid spin boxes in the Reference Positions frame to 1 and
41, respectively.
4. With the Use for Y option buttons, select the grid position
where the Y-range will be determined. Use for Y is set to
Left by default.
5. Start the procedure with Start.

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• If there is only one reference tip available, the


software prompts when to mount it to the first tip
position.
• If the 4/8 Reference Tips Available checkbox on the
Setup page is selected, a prompt displays to mount a
reference tip to each tip position.
6. Ensure the reference tip(s) are mounted to the required
position(s) then confirm with Done.

7. Starting with the first position, one tip adapter after the
other is moved downward until the reference tip mounted
to it touches the worktable surface. After the Z-range is
determined, the respective tip is moved up again.
• If there is only one reference tip available, the
software prompts when to attach it to the next
position. Confirm with Done. The program continues
with the next position.
• If there is a reference tip at each position, the software
automatically continues with the next position.
8. After the Z-ranges of all tip positions are determined, the
operator is prompted to remove the reference tip(s).

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Section 11 Liquid Handling Arm (LiHa)

9. Confirm with OK. The procedure is completed and the


values are written to the LiHa.

Determine Reference Positions


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Determine Reference Positions procedure moves the
LiHa with the driven tips to the selected reference positions in
sequence.
• To adjust tips and to calibrate the X- and Y-axes.
• To write the results to the LiHa.
NOTE: The “driven” tips are tips 1 and 3 on 4-tip
instruments, tips 3 and 7 on 8-tip instruments.

Reference Positions

Reference Tip

Reference Point

Reference Pin on Worktable

Figure 11.41: Reference Position

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Procedure
Perform the following steps to determine the reference
positions.
1. On the Contents page select the Determine Reference
Positions checkbox (聺) and switch to the Setup page
(Figure 11.11.40 on page 11-119).

2. Select the 4/8 Reference Tips Available checkbox (聺) if


there is a reference tip for each required tip position, if not
clear it (□).
NOTE: There are two reference tip types available.
Refer to Reference Tips on page 4–54 of Section 4.
3. In the Determine Reference Positions frame, select the
mode to determine the reference positions:
• Select the Manually option if the reference positions
will be determined manually. Select the Verify with
mounted tip option if an “ordinary” tip will be used
for the verification instead of a reference tip.
• Select the Automatically option button if the
reference positions will be determined automatically.
Automatically is the default setting.
4. In the frame Reference Positions, set the Left and Right
grid positions in the corresponding Grid spin boxes 1
and 41, respectively.
5. Select the grid position needed to determine the Y-range
with the Use for Y option buttons. Use for Y is set to Left
by default.
6. Start the procedure with Start.

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Section 11 Liquid Handling Arm (LiHa)

• If there is only one reference tip available, a prompt


displays to mount it to the first tip position,
position 1 on a 4-tip instrument, position 3 on an
8-tip instrument.
• If the 4/8 Reference Tips Available checkbox (聺) is
selected, a prompt displays to mount a reference tip to
the required tip positions. Ensure there are reference
tips at positions 3 and 7.
7. Confirm the reference tip(s) is in the correct position(s)
with Done.
8. If Automatically is selected on the Setup page, the driven
tips search the reference positions automatically.
• If only one reference tip is mounted, the software
prompts when to mount it to another position.
• Continue with step 13.
9. If Manually is selected on the Setup page, the Move LiHa
page is activated (refer to Move LiHa Page, page 11-114)
and the message Use move tool to determine first
reference point displays.
10. Adjust the position in X- and Y-direction according to
Figure 11.41.
• If only one reference tip is mounted, the software
notifies when it should be mounted to another
position.
11. If the reference tip is in the correct position, confirm with
Done.
12. Repeat steps 10 and 11 for all required positions.
13. After checking all reference positions, the message Please
remove reference tips displays. Confirm with Done.

Determine Spacing
Based on results of the previous procedure, spacing is
calculated automatically. No further adjustments are required.
The results are written to the LiHa.

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Individual Z Alignment

Purpose
This alignment is not used when running m2000sp software.
Additional alignments on the worktable are performed using
m2000sp software. The purpose of the Individual Z is to set
the bottom ends of non-active tips to the same height.

Check Tip Adapter

Purpose
The Check Tip Adapter function checks the electrical contact
for the DiTi and Liquid Detection functions.

Procedure
Perform the following steps to check the tip adapters.
1. On the Contents page, select the Tip Adapter checkbox
(聺).
2. Remove all DiTis from the DiTi adapters.
3. Switch to the Test Configuration page (refer to Figure
11.42).

Figure 11.42:LiHa Test Configuration Page, Tip


Adapter Test

4. Select ALL to test all DiTi adapters and select Start.


5. A prompt displays to move up the first tip adapter.
6. Push it upward and hold in the contact position.

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7. Observe the process prompt displayed on the screen.


When the tip adapter is in the contact position, the blue
progress bar must display on the process prompt as
illustrated.

Figure 11.43:Tip Adapter Test

8. If the tip adapter works properly, confirm with Next. If


not, select Cancel.
9. Repeat steps 6 through 8 until all tip adapters are tested.

Calibrate the Lower DiTi Eject Protocol


1. Initiate the procedure by selecting <System
Device><LiHa>. The LiHa Panel/Contents page displays.
2. Select the Calibrate Lower DiTi Eject checkbox (聺).

Figure 11.44:LiHa Panel (Built In)

3. Select Start.

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4. Enter the serial number of the instrument in the Device


Serial Number field.

Figure 11.45:Enter Serial Number Dialog

5. The test proceeds automatically and requires no user


intervention.
6. At the LiHa Process Prompt screen, select OK.

7. Select Close again to return to the Main menu.

Dilutor Tool

Description
The Dilutor tool checks the dilutors and associated tubings.
Each dilutor can be activated individually. For example, this
tool allows the FSE to determine the following:
• If the dilutor address switches are set correctly. For
example, if the same address is assigned to two different
dilutors, one of them cannot be activated.
• If each dilutor is connected (via the tubing) to the correct
LiHa channel. For example, if liquid coming from
dilutor #1 is dispensed through tip #3, it is connected to
the wrong channel.

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• The Dilutor tool can also be used to fill/flush the liquid


systems with system liquid connected, or empty the liquid
system with the system liquid supply disconnected.
The Dilutor tool does not set or adjust any values stored in
memory. No password is required to operate the Dilutor tool.
The Dilutor tool is subdivided into eight channels associated
with the corresponding dilutors, tubings, and tips. The control
elements of those channels at which no dilutor is recognized
(because it is not installed, wrongly addressed or otherwise
malfunctioning) are not enabled.

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Genesis Setup and Service Software Section 11

Controls
The Dilutor tool includes the following controls.

Control Description
Sliders 1 to 8 There is one slider per channel. The sliders
aspirate and dispense liquid through the
associated dilutors.
Init buttons 1 to For initializing dilutors individually.
8
Text fields 1 to 8 Displays the volume, in µL, aspirated or
Volume to dispensed by the respective dilutor. If the
aspirate/dispen indicated value is positive, the indicated
se [µL] liquid quantity is aspirated; if negative, it
is dispensed.
Flush tips once Initializes the dilutors one by one. The
sequentially syringe volume is aspirated then dispensed
through the respective tip.
Flush Flushes or empties the liquid system.
Wash station Location of the wash station.
Grid:
Position: Position of the wash station.
Use Fast Wash Select to use the FaWa pump to flush.
Pump:
Fill Duration Duration of the filling.
Flush Initialize the flush protocol.

NOTE: When the Dilutor tool is used, the selected


dilutor always aspirates system liquid and dispenses it
through the associated tip. It never aspirates liquid
through the tip, and therefore does not contaminate
the system liquid.

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Section 11 Liquid Handling Arm (LiHa)

Initializing the Dilutors


NOTE: It is necessary to install a wash station on the
worktable at grid 18, and to move the LiHa over the
wash station before initializing or testing the dilutors.
This ensures that liquid dispensed during the
subsequent initialization and test procedures does not
splash over the worktable.
The dilutors must be initialized before they are used.
1. Select the Init button of the respective channel. Only one
channel at a time can be selected.
NOTE: Depending on the dilutor type, initialization
can take time. It is possible for liquid to be aspirated
and dispensed during initialization.

Aspirating and Dispensing with Dilutors


The arrows in Figure 11.46 indicate the direction a slider must
be dragged to aspirate or dispense liquid.
Down arrow—determines the system liquid quantity
aspirated.
Up arrow—determines the quantity dispensed.
Refer to the following procedures for details.

Figure 11.46: Aspirating/Dispensing Liquid

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Genesis Setup and Service Software Section 11

Perform the following steps to aspirate system liquid.


1. Use the mouse to point to the respective slider and drag
the slider downward () until the text box below displays
the desired volume.
2. Release the mouse button. The dilutor aspirates the
defined quantity of system liquid.
3. Verify the correct dilutor is activated.
• If slider #1 was dragged, the left-most dilutor must
aspirate liquid.
• If slider #8 was dragged, the right-most dilutor must
aspirate liquid.
Perform the following steps to dispense aspirated liquid.
1. With the mouse, point to the desired slider and push it
upward () until the associated text box displays the
required quantity.
2. Release the mouse button. The dilutor presses the defined
quantity through the respective tubing and tip into the
wash station.
3. Verify the correct dilutor is activated and the liquid
emerges from the correct tip.
• If slider #1 was pushed up, liquid must be pressed out
of the rear-most tip (channel #1).
• If slider #8 was pushed up, liquid must emerge from
the front tip (channel #8).

Error Handling
Errors occur:
1. If the wrong dilutor reacts, check the address settings.
Note the address number corresponds to:
• Dilutor # minus 1 (i.e., dilutor 1 has address 0, dilutor
8 has address 7).
2. If liquid emerges from the wrong tip, check the
connection of tubing between the dilutors and tips.
3. Check the tubing for leaks, and to verify they are not
kinked or damaged. Replace damaged parts.

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Section 11 Liquid Handling Arm (LiHa)

Flushing Dilutors Sequentially


The large command button Flush tips once sequentially
below the text boxes allows aspiration and dispense of liquid
sequentially. When this button is selected:
• Dilutor #1 (on the left side) is initialized. After
initialization, it aspirates 1/3 of the maximum syringe
volume (e.g., if the syringe volume is 1000 µL, 330 µL is
aspirated) and dispenses the aspirated quantity.
• The program continues with the next dilutor until all
dilutors recognized by the software are flushed.

Perform the following steps to flush the dilutors.


1. Select the Flush tips once sequentially button.
2. The prompt Do you want to move the LiHa above
Washstation? displays. Select Yes.

3. The prompt Please set Grid Position of Washstation


displays. Select OK.

4. Set the Washstation Grid Location to 18 and the


Position to Waste.
5. Select Flush tips once sequentially.
6. The protocol initiates and flushes each tip sequentially.
Perform the following steps to flush all tips together at one
time.
1. Under Flush Tips, select the Use Fast Wash Pump
checkbox (聺).
2. Enter the fill duration.
3. Select Flush.
4. All eight tips are flushed.

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Genesis Setup and Service Software Section 11

Filling/Emptying Liquid from Instrument


The LiHa Dilutor page is also used to fill or empty liquid from
the instrument.
1. Connect (disconnect to empty) the tubing at the system
liquid container.
2. Select <LiHa><Dilutor> from the menu.

3. Set the Wash Station Grid to 18 and the Position to


waste.
4. Select Use Fast Wash Pump.
5. Set the Duration to 10 seconds.
6. Select Flush.
7. For emptying: repeat the protocol if necessary, with a
shorter time duration to flush any liquid remaining in the
instrument.

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Section 11 Liquid Handling Arm (LiHa)

Information Page

Purpose
The Information page summarizes device parameters and
diagnostics data.

Figure 11.47: Information Page

Data
The Information page includes the following controls.

Control Description
Refresh Reloads the diagnostic data and refreshes
the Sample Time.
Sample Time Snap shot time, e.g., time when the
diagnostic data were read.
Device Parameters
Axes Movement axis: X, Y, Ys, Z1 to Z8.
Offset Offset for each axis, in mm.
Displ Displacement for each axis, in mm.
Range Range for each axis, in mm.
Scale Scale factor.
Accel Acceleration for each axis, in mm per
square sec.

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Genesis Setup and Service Software Section 11

Control Description
Speed High speed of each axis, in mm per sec.
Move Speed Working speed of each axis, in mm per
sec.
Diagnostics Data
Moves Number of overall moves per axis.
Moves Number of clearable moves per axis.
Distance Total moved distance, in meters, per axis.
No-Loads Total number of no-loads, e.g., axis
crashed.
Fetched DiTis Total number of fetched DiTis per tip.
Piercings Total number of piercings per tip.
Status The status of the axis in question (ready,
initialization error, not implemented,
not initialized, not loaded, unknown).

Viewing Data
Perform the following steps to access the Information page.
1. Switch to the Information page (Figure 11.11.47 on page
11-133).
2. Select Refresh.
3. View the data.
4. Print the setup results with Print or preview the Test
report (Printing, page 11-135).

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Section 11 Liquid Handling Arm (LiHa)

Printing

Purpose
The Printing page allows formatting of a Test report to meet
laboratory requirements.

Figure 11.48: LiHa Panel - Printing Page

Controls
The Printing page includes the following controls.

Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Device Checkbox to select device data.
Configuration Checkbox to select configuration data
as shown on the Information page
(Figure 11.47).
Parameters Checkbox to select device parameters
as shown on the Information page
(Figure 11.47).
Diagnostics Checkbox to select diagnostic data as
shown on the Information page
(Figure 11.47).
Setup Results For printing the setup results.

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Genesis Setup and Service Software Section 11

Control Description
Tip Adapter Checkboxes to select tip adapter
results.
Detailed
Results
Arm Position Not relevant (manufacturer
Accuracy procedure).
Multiple existing Data selection valid for all device data
Sections selected in the Detailed Print Section
Configuration section.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to
setup a printer.

Printing a Report
Perform the following steps to print a report.
1. Switch to the Printing page (Figure 11.48).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the Test report onscreen with Result Preview.
4. Select Print to print the Test report.

Test Report
Depending on selections, the Test report includes the
following data.

Description
Header Global data such as software versions,
LiHa and instrument serial numbers, date
of creation, and global test review (not
relevant).
Signature section with operator name.

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Section 11 Liquid Handling Arm (LiHa)

Description
Device
Configuration Address, firmware and bootware version,
number of tips, spacing.
Device Axes, offset, displacement, range, scale,
Parameters acceleration, speed, move speed.
Diagnostics Same values as on the Diagnostics page:
Axes, moves, distance, no-loads, fetched
DiTis, piercings, status.
Setup Results
Deviation: Shows deviations of the arm for
movements in xa and y directionb.
Arm Position Not done (manufacturer procedure).
Accuracy:
Tip Adapter Detailed results, test configuration,
configuration details.
a. Deviation in X-axis: Note that two values are shown, one
per driven tip (tips 1 and 3 on 4-tip instruments, tips 3 and
7 on 8-tip instruments).
- If the X-deviation is 0, the arm moves exactly at a right
angle (90°) to the front edge of the worktable.
b. Deviation in Y-axis is 0 if the arm moves exactly parallel to
the worktable in the X-axis.

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Genesis Setup and Service Software Section 11

Move Test
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instruments safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

Purpose
The Move Test function verifies correct functioning of the
drives of the LiHa, RoMa, PosID, and Dilutors. For other
options, a range test (Range Test, page 11-140) and/or a
Random Move Test (Random Move Test, page 11-141,
corresponds to the burn-in or validation procedure) can be
performed.
NOTE: Before starting the Move Test function, ensure
the worktable is empty and free of obstacles at all times
since the drives move with high speed.

Panel Description
The Move Test function provides two procedures to be
executed. Generally, the following steps must be performed:
1. Start the Move Test panel  Move Test Panel, page 11-139.
2. Select the test to be performed and modify the
corresponding settings, if necessary.
NOTE: Before starting any test procedure, the Operator
field on the Printing page should be completed.
3. Start the desired procedure with Start.
4. After performing any test, check the test results  Results,
page 11-143.
5. Print the test results  Printing, page 11-146.
6. Finish with Close.

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Section 11 Move Test

Files, Directory
The Move Test function creates the following files. Refer to
Section 4, Installation for additional information.

Description
Result files Directory C:\Program
Files\Tecan\Genesis
Instrument Software\Results
Name MoveTest_<serial_number>_<
date>_<time>.any
Configuration Directory C:\Program
files Files\Tecan\Genesis
Instrument Software
Name <name>.anya
a. Enter the full file name (with extension.any)

Move Test Panel

Figure 11.49: Move Test Panel - Contents Page

Pages
The Move Test window is subdivided into the following pages.

Page Description
Contents Test selection.
Test Configuration of the random move test.
Configuration
Results Test results summary; refer to Results, page
11-143.
Printing Print selection for the Test report; refer to
Printing, page 11-146.

It also includes the standard command buttons.

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Genesis Setup and Service Software Section 11

Controls
The Contents page includes the following controls.

Control Description
Range Performs a Range test; refer to Range Test,
page 11-140.
Random Performs a Random Move test; refer to
Random Move Test, page 11-141.

Using the Panel


Perform the following steps for the Range or Random Move
test.
1. Start the panel with System Devices > Move Test. The
Move Test Panel window with activated Contents page
(Figure 11.49) displays. After starting the Move Test
function, no checkbox is selected and not all tabs are
visible.
2. Select the test to be performed, and define the necessary
data as described in the given section.
3. Before starting any test procedure, switch to the Printing
page (Figure 11.53) to complete the Operator and
Comment fields.
4. Start the desired procedure with Start.
5. Switch to the Results page to check the test results
(Results, page 11-143) or switch to the Printing page to
preview the Test report (Printing, page 11-146).
6. Finish with Close.

Range Test
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Range Test procedure determines if the installed LiHa,
RoMa, and PosID can reach the whole range. Each device
moves along its axes to its extreme positions. The evaluated
range is compared to the stored ranges. The tests pass if the
difference between the determined and stored values are
within the deviation limits. The test lasts approximately 5 to
10 minutes, depending on the installed devices.

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Section 11 Move Test

Before Starting Range Test


The Range Move test can be performed with the DiTi adapters
mounted.

Procedure
Perform the following steps for the Range test.
1. Select the Range checkbox (聺). No other parameters must
be defined.
2. Start the procedure with Start. The message Ranges Move
Test progress displays.
3. At the end of the test, a message Range Move Test: Passed
or Range Move Test: Failed displays.
4. Confirm with OK.

Pass/Fail Criteria

The test passed if the evaluated Deviation does not exceed the
specified Deviation Limit, e.g., Range = ok.
If the test fails:
• For the LiHa, perform the Determine Range and Spacing
procedures in this section.
• For the RoMa, perform the Determine Range procedure in
this section.
• For the PosID, perform the Autorange procedure in Setup
Procedure, page 11-76.

Random Move Test


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

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Genesis Setup and Service Software Section 11

Purpose
The Random Move Test determines if the mechanics of the
LiHa, RoMa, and PosID work properly. All devices are moved at
random for the defined number of cycles; the default setting is
7500 and lasts approximately 8 to 16 hours. After a defined
number of moves (default setting is 200), an initialization is
performed and the number of lost steps is evaluated for each
axis.

Before Starting Random Move Test


• Ensure the worktable is empty and free of obstacles at all
times.
• The DiTi adapters can remain mounted.
• If the RoMa is equipped with eccentric gripper fingers,
remove them from the gripper module.

Procedure
Perform the following steps for the Random Move test.
1. Select the Random checkbox (聺) and switch to the Test
Configuration page (Figure 11.50).
2. Enter the number of cycles to be performed in the
Duration field. The default setting is 7500, which lasts
approximately 8 to 16 hours.

Figure 11.50:Move Test Panel - Test Configuration


Page

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Section 11 Move Test

3. Enter the number of cycles then an initialization is


performed in the Re-Init Interval field. The default setting
is 200.
4. Start the procedure with Start. The process prompt
Random Move Test displays.

5. At the end of the test, the message Random Move Test:


Passed or Random Move Test: Failed displays.
6. Confirm with OK.

Pass/Fail Criteria

The test passed if the evaluated Maximal Deviation does not


exceed the specified Deviation Limit, e.g., if Exceed
Counter = 0.
If the test fails, perform the corresponding setup functions.
There is a mechanical problem if the test fails again.

Results

Purpose
The current test results are summarized on the Results page.
Using the Test Results option buttons, results for the
corresponding test can be displayed.
Perform the following steps to view the results.
1. Switch to the Results page (Figure 11.51).
2. Use the option buttons in the Test Results frame to select
results to view. The Range option button is selected by
default.
3. Print the setup results with Print or preview the Test
report (Printing, page 11-146).

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Genesis Setup and Service Software Section 11

Results of Range Test

Figure 11.51: Move Test Panel - Results Page for Range Test

The Results page, with the Range option button selected,


displays the following data.

Description
Device Name and address of the tested device.
Axis Tested axis.
Range Result of Range Move test. Tip mounted
indicates the corresponding Z-axis could not be
tested for the whole range, because a tip was
mounted.
Deviation Difference between the distance evaluated
during initialization and the expected distance.
Deviation Allowed difference between the evaluated and
Limit expected distance.
Within Compare result for Deviation less than
Limit Deviation Limit.

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Section 11 Move Test

Results of Random Move Test

Figure 11.52: Move Test Panel - Results Page for Random


Move Test

The Results page, with the Random option button selected,


displays the following data.
Description
Device Name and address of the tested device.
Axis Tested axis.
Maximal Biggest difference between the distance
Deviation evaluated while initialization and the
expected distance.
Deviation Allowed difference between the evaluated
Limit and expected distance.
Exceed Incremented each time the allowed
Counter Deviation Limit is exceeded.

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Genesis Setup and Service Software Section 11

Printing

Purpose
The Printing page allows formatting of a Test report to meet
laboratory requirements.

Figure 11.53: Move Test Panel - Printing Page

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Section 11 Move Test

Controls
The Printing page includes the following controls.

Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Range Move Test Checkboxes to select the tests to print.
Random Move Test Each test contains a substructure (expand
with [+]) with the same data selection
properties as the Multiple existing
Sections section.
Multiple existing Data selection valid for all tests selected in
Sections the Detailed Print Section Configuration
section.
Detailed Results Checkbox to print detailed test results.
several devices or Checkbox to print the test results for a
axis specific device or axis.
Operator Field for operator name. An entry is recommended.
Comment Field for additional notes. An entry is recommended.
Result Opens a Test report preview window.
Preview
Printer Setup Starts the standard Windows dialog to setup a printer.

Printing a Report
Perform the following steps to print a Test report.
1. Switch to the Printing page (Figure 11.53).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the Test report onscreen with Result Preview.
4. Print the Test report with Print.

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Genesis Setup and Service Software Section 11

Test Report
Depending on selections, the Test report includes the
following data.

Test Report
Header
Global data, e.g., software versions, instrument serial number, date of
creation, and global test review such as:
Test Result: Passed, if all tests passed; if not, Failed.
Tests done: All or Not all
Test Configuration:Default or User Defined/User Defined [<file
name>], if there were deviations to the default values.
Device Default Settings: not relevant.
Signature section with operator name.
Range Move Test: Passed or Failed or Not Done
Detailed Results Same data displayed on the Results page,
(Results of Range Test, page 11-144)
subdivided into devices.
Random Move Test: Passed or Failed or Not Done
Test Configuration Detailed configuration data.
Details
Detailed Results Same data displayed on the Results page,
(Results of Random Move Test, page 11-145)
subdivided into devices.

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Section 11 Robotic Manipulator Arm (RoMa)

Robotic Manipulator Arm (RoMa)


WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

Purpose
The Robotic Manipulator arm (RoMa) is used to transport
objects of the format of microplates, such as reagent blocks,
deep well plates, etc. to different positions or for storage onto
the shelf.
The RoMa types, gripper fingers, and rotation
directions—described in the following sections—are important
for correct setup of the RoMa.

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Genesis Setup and Service Software Section 11

RoMa Types
• RoMa with eccentric gripper fingers

Figure 11.54:RoMa for m2000sp Instrument

RoMa for Freedom. Two types of RoMa can be installed


on Genesis Freedom instruments. Note they cannot be
installed on a Genesis Classic, because a different X-drive
is required.
• RoMa Freedom (Figure 11.55) is used for plate
handling on the worktable. This type is normally
equipped with eccentric gripper fingers.
• RoMa Long with long Z-axis for plate handling on and
underneath the worktable (e.g., for loading a
centrifuge placed underneath the instrument). RoMa
Long is equipped with centric or eccentric gripper
fingers.
• The m2000sp instrument is configured with the RoMa
Long with the eccentric gripper fingers.

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Section 11 Robotic Manipulator Arm (RoMa)

• RoMa Freedom with eccentric gripper fingers

Figure 11.55:RoMa for m2000sp Instrument

Movement Axes
The RoMa’s movements are coordinated with other system
devices such as the LiHa. There are five different movement
axes.
Axis Description
X-axis Movement of the RoMa left-right.
Y-axis Movement of the RoMa forward-backward.
Z-axis Movement of the RoMa gripper head
up-down.
G-axis Movement of the RoMa gripper fingers
open-close.
R-axis Rotational motion of the RoMa gripper
head.

Rotation Directions
Possible rotation directions depend on the RoMa type:
• The RoMa Long can be set mechanically to rotate two
directions:
CW (clockwise) within a range 0° to 270°, start position: 0°
(Figure 11.56, right side). Used for the m2000sp instrument.

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Genesis Setup and Service Software Section 11

– CCW (counterclockwise) within a range 360° to 90°,


start position: 360° (Figure 11.56, left side). Not used
with the m2000sp instrument.

90° 270°

Counterclockwise (CCW) Clockwise (CW)


360° 0°

Figure 11.56:RoMa Rotation Directions

Y Z
G
X

X Arm movement: left–right Z Gripper head movement:


Y Arm movement: for- up–down
ward–backward G Gripper finger: open–close
R Gripper head: axis of rota-
Figure 11.57:RoMa Movement Axes

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Section 11 Robotic Manipulator Arm (RoMa)

Gripper Types
Different types of gripper fingers can be mounted on the
rotator of a RoMa.
• Eccentric gripper fingers, Figure 11.58, can be used for all
types of RoMa.

Figure 11.58:Gripper Types - Eccentric Gripper Fingers

NOTE: This configuration is used for the m2000sp


instrument.
• Centric gripper fingers, Figure 11.59

Figure 11.59:Gripper Types - Centric Gripper Fingers

NOTE: Not used with the m2000sp instrument.

Required Tools
The RoMa Reference tool and the RoMa Test Plate (Figure
11.60) are required for RoMa function.

Reference Tool
Test Plate

Figure 11.60: RoMa Reference Tools and Test Plate

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Genesis Setup and Service Software Section 11

The eccentric gripper fingers do not require removal to install


the reference tool.

Panel Description
The RoMa panel provides several procedures to be executed.
Generally, the following steps must be performed:
1. Start the RoMa panel  Move Test Panel, page 11-139‘’.
2. Select the setup procedures to be performed (refer to Table
61) and modify the corresponding settings.
There are two ways to proceed:
• Select ALL Setup checkboxes (聺) at the same time and
enter the necessary data by clicking the corresponding
tabs. Press Start and all setup procedures are
performed in one run.
• Select one or more Setup checkboxes (聺) and define
the necessary data as described. When Start is pressed,
the currently selected setups are performed.
• If the second method is selected, start with the
topmost setup procedure and work downward. Use
the second method to repeat an individual setup.
• Before starting any setup procedure, the Operator
field on the Printing page should be completed.
3. Start the desired procedure with Start.
4. After performing any setup, check setup results  Results,
page 11-143.
5. Print setup results  Printing, page 11-146.
6. Finish with Close.

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Section 11 Robotic Manipulator Arm (RoMa)

Procedures, User Permissions


Table 11.61: RoMa procedures and user permission
Refer to
Operator FSE
Section
Determine Range. Remove X
gripper fingers if necessary!
Set Type X
Determine Reference X
Positions
Adjust Rotator X
Move RoMa X X
Results X
Printing X X
Test Report X X

Files, Directories
The RoMa function creates the following files.

Description
Saved in C:\Program Files\Tecan\Genesis
directory: Instrument Software\Results
File name: RoMa_<serial_number>_<date>_<time>.a
ny

RoMa Panel

Figure 11.62: RoMa Panel - Contents Page

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Genesis Setup and Service Software Section 11

Pages
The RoMa window includes the standard command buttons,
and is subdivided into the following tabs.

Tab Description
Contents General overview, setup selection.
Setup RoMa configuration.
Test For configuring the Reference Plate test.
Configuration
Printing Print selection for the Test report (Test
Report, page 11-172).
Move RoMa Movement tool (Move RoMa, page 11-158).
Information Device parameters and diagnostics data
(Information Page, page 11-169).

Controls
The Contents page includes the following controls.

Control Description
Available List of connected RoMas (two RoMas
Devices possible on a Genesis Freedom).
Set Default Sets default values.
Parameters
Determine Determines Y and Z range; refer to
Range Determine/Check Range, page 11-161.
Set Type Sets the RoMa type; refer to Set Type, page
11-163.
Determine Determines or checks reference positions;
Reference refer to Determine Reference Positions, page
Positions 11-164.
Adjust Rotator Adjusts the rotator’s offset and scaling; refer
to Adjust Rotator, page 11-168.
Reference Tests if plates are picked up/placed correctly.
Plate

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Section 11 Robotic Manipulator Arm (RoMa)

Using the Panel


CAUTION: Because the RoMa moves at high speed,
ensure the worktable is empty and free of obstacles at
all times. Keep the safety door up. Door locks do not
need to be removed.
Ensure the instrument was setup with <Instrument>
<Basic Setup>.
Perform the following steps to setup the RoMa.
1. Start the panel with <System Devices><RoMa>. The
RoMa Panel window with activated Contents page
(Figure 11.49) displays. After starting the RoMa function,
no checkbox is selected and not all tabs are visible.
2. If there is more than one device listed in the Available
Devices section (possible on a Genesis Freedom
instrument), select the desired RoMa to setup.
3. Select the setup procedures to be performed and define
necessary data as described in the given section. All setup
procedures can be selected, or one or more for the desired
run. Refer to Panel Description, page 11-154 for details.
4. Before starting any setup procedure, switch to the
Printing page (Figure 11.53) to complete the Operator
and Comment fields.
5. Start the desired procedure with Start.
6. Switch to the Results page to view the setup results
(Information Page, page 11-169) or switch to the Printing
page to preview the Test report (Test Report, page 11-172).
7. Finish with Close.
CAUTION: If there is a RoMa listed without a valid
serial number, it is not automatically selected. A
selection is only possible if a valid serial number was
previously entered (requires the FSE password).

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Genesis Setup and Service Software Section 11

Move RoMa
This function works only if the RoMa was previously selected
on the Contents page.

Purpose
The Move RoMa page allows movement of the whole RoMa,
or parts of it in the appropriate axes: X, Y, Z, G and R..

Controls
The Move RoMa page includes the following controls.

Control Description
Single step, in 1/10 mm or 1/10 degree,
movement/rotation buttons.
Ten steps, in 1 mm or 1 degree,
movement/rotation buttons.
Continuous movement/rotation buttons
when button is kept pressed.
To logical positions (90°/180°/270°) rotation
buttons.
Free Moves all arms to their extreme positions.

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Section 11 Robotic Manipulator Arm (RoMa)

Control Description
Grip Grips the test plate.
X, Y Text boxes indicating the current position of
the rotation axis with respect to grid
position #1.
Z Current position in Z-axis (distance of
grippers from worktable).
R Current rotational angle of gripper module.
G Current distance between grippers.

• Arrows on the movement buttons indicate the direction in


which the respective parts are moved.
• If the mouse pointer is placed over a movement button,
text displays to explain the button’s function (“tool tip”).

Keyboard Control
It is possible to also use the keyboard for movements in the
various axes.

Setup Page

Purpose
The Setup Page provides controls for setting parameters for the
various setup procedures.

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Genesis Setup and Service Software Section 11

Controls
The Setup page includes the following controls.

Control Description
Rotator Type For specifying the rotation direction.
Gripper Type For specifying the gripper type.
Z-range For specifying the Z-range.
Reference Frame for setting the reference positions.
Positions
Grid Spin boxes for setting left and right
reference grid positions.
Use for Y Option buttons to specify if reference
positions in the Y-axis should be
determined at the left or right reference
position.
Ref. Plate Pos. Spin box to set the grid position of the
Grid reference plate.
Initialize Spin box used if a Te-MO is installed on
Position X the instrument. Defines the position
(relative to grid position #1) at which the
arm is initialized. The position must be
chosen to eliminate possible collisions
with the Te-MO. The position can be set
with an accuracy of 0.1 [mm].
Table on right Current speed and accelerations in X-, Y-,
side Z-, R-, and G-axes.

NOTE: Ensure all parameters are set correctly.

Refer to the respective setup procedures for additional


information regarding entries to be made.

Set Default Parameters


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

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Section 11 Robotic Manipulator Arm (RoMa)

NOTE: Do not start this function unnecessarily. After


performing this function, it is necessary to readjust all
ranges, reference positions, rotation ranges etc.).

Purpose
This function resets reference positions, ranges, and offsets to
the original (factory-set) values. It should be used only:
• When a new instrument is set up the first time
• After certain repairs from which maladjustment could
result (e.g., removal and reinstallation of the worktable,
replacement of whole RoMa etc.).

Procedure
Perform the following steps to set the default parameters.
1. On the Contents page select the Set Default Parameters
checkbox (聺) and select Start.
2. When finished, all ranges, reference positions, and
rotation ranges with the respective functions must be
adjusted.

Determine/Check Range
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Determine Range procedure moves the RoMa in Y-, Z-, G-,
and R-direction to its extreme positions to find the available
ranges. The determined values are then written to the RoMa.
To mount the reference tool, the gripper fingers must be
removed from the rotator. Refer to Table 61.

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Genesis Setup and Service Software Section 11

Procedure
Perform the following steps to determine the range.
1. On the Contents page, select the Determine Range
checkbox (聺) and switch to the Setup page.

Figure 11.63:RoMa Panel - Setup Page

2. In the Reference Positions frame, configure the


following:
• Use the Grid spin boxes to set the left and right grid
positions to 7 and 45, respectively.
• Select the appropriate Use for Y option button to
select the reference position (Left or Right) where the
Z-range will be determined.
3. Perform the procedure by selecting Start. The message
Mount Reference Tool displays.
4. Hold the reference tool against the gripper module and
select OK. This opens the grippers to hold the reference
tool.
5. Confirm the message Ready to proceed with OK.
6. After determining the range, the message Unmount
Reference Tool displays.
7. Hold the reference tool in the gripper then select OK. The
reference tool is released and the determined values are
written to the RoMa.
8. Select OK at the prompt then select OK again at the
Setup:Done prompt.

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Section 11 Robotic Manipulator Arm (RoMa)

Set Type
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Set Type procedure allows definition of the following:
• Rotation direction of gripper head
• Gripper finger types
• RoMa type to set up (RoMa Standard or RoMa Long)

Procedure
Perform the following steps to set the RoMa type.
NOTE: Refer to Orientation RoMa Standard on page
3–12 of Section 3, Technical Data to verify screw
position for CW rotation.
1. Select the Set Type checkbox (聺) and switch to the Setup
page (Figure 11.50).

Figure 11.64:RoMa Panel - Setup Page

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Genesis Setup and Service Software Section 11

2. Use the option buttons in the Rotor Type, Gripper Type,


and Z Range frames to make the selection. Refer to the
following table.

Description
Rotator Type Configures the rotator type.
CW Select this option button so the gripper
head rotates clockwise. The m2000sp
instrument must be set to CW.
CCW Select this option button so the gripper
head rotates counterclockwise. Not used
with the m2000sp instrument.
Gripper Type Configures the gripper type.
Eccentric Select this option button to define
installation of the eccentric gripper
fingers. The m2000sp instrument uses
eccentric gripper fingers.
Centric Select this option button so the centric
gripper fingers are installed. Not used
with the m2000sp instrument.
Z Range Configures the Z-range of the gripper
arm.
Normal Option button to define the RoMa in
(standard) question as a RoMa Freedom (with a
standard Z-drive). The m2000sp
instrument uses a RoMa standard.
Extended Option button to define the RoMa in
(long) question as a RoMa Long (with a long
Z-drive).
Reference Not used in this context; refer to Adjust
Plate Rotator, page 11-168.
Position

3. Save the selections with Start. A message with the changes


displays.
4. Confirm the changes with OK. The values are written to
the RoMa.

Determine Reference Positions


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

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Section 11 Robotic Manipulator Arm (RoMa)

Purpose
The Determine Reference Positions procedure moves the
RoMa to the selected reference positions in sequence:
• To adjust and determine the X- and Y- scale factors, and
X- and Y-displacements.
• To check reference positions.
• To mount the Reference tool, the gripper fingers must be
removed from the rotator as illustrated below.

Reference Tool

Reference Pin

Figure 11.65:Reference Position

Procedure
Perform the following steps to determine reference positions.
1. Select the Determine Reference Positions checkbox (聺)
and switch to the Setup page.

Figure 11.66:RoMa Panel - Reference Positions Page

2. Set the Left and Right reference position for the RoMa at
7 and 45, respectively.
3. With the Use for Y option button, select the Left or Right
reference position for movement in Y-direction.
4. Start the procedure with Start. The message Mount
Reference Tool displays.

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Genesis Setup and Service Software Section 11

5. Hold the reference tool against the bottom of the gripper


module then select OK. This opens the gripper to hold the
reference tool.
6. Confirm the message Ready to proceed with OK. The
Move RoMa page is active (refer to Move RoMa, page
11-158) and the message Use move tool to determine
first reference point displays.
7. Adjust the position in X- and Y-direction according to
Figure 11.65.
8. If the reference tool is in the correct position, confirm with
Next.
9. Repeat steps 7 and 8 for all indicated positions.
10. After all reference positions are adjusted, the message
Unmount Reference Tool displays.
11. Hold the reference tool in the gripper then select OK. The
reference tool is released and the values found are written
to the RoMa.
12. Select OK to proceed.
13. Select OK at Setup:Done prompt.
14. If the RoMa is significantly out of alignment or the test
fails, reset the default parameters and perform the Set
Type, Determine Range, Determine the Reference
Positions, and Adjust the Rotator procedures.
NOTE: Be sure to reinstall gripper fingers. However, if
the Determine Range procedure in this section is
performed, it should be performed before mounting the
gripper fingers.

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Section 11 Robotic Manipulator Arm (RoMa)

Check Reference Positions


The following procedure is similar to the one used to
determine the reference positions, described earlier in this
section. The difference is that the procedure described in this
section only checks the reference positions, but does not
readjust them.
Perform the following steps to check the reference positions.
1. On the Contents page, select the Determine Reference
Positions checkbox (聺) and switch to the Setup page
(Figure 11.66).
2. Set the Left and Right reference positions at 7 and 45,
respectively.
3. With the Use for Y option buttons, select the Left or Right
reference position for movement in Y-direction.
4. Start the procedure with Start. The message Mount
Reference Tool displays.
5. Hold the reference tool between the grippers and select OK
to grip the reference tool.
6. Confirm the message Ready to proceed with OK. The
Move RoMa page is activated (refer to Move RoMa, page
11-158) and the message Use move tool to determine
first reference point displays.
7. Check the reference position using only the command
buttons in Z-direction. If the command buttons are not
used for X- and Y-direction, adjustment of the reference
positions is not changed.
8. To check the next position, confirm with Next.
9. Repeat steps 7 and 8 for all indicated positions.
10. After all reference positions are checked, the message
Unmount Reference Tool displays.
11. Hold the reference tool in the gripper then select OK. The
reference tool is released.
NOTE: Remember to reinstall gripper fingers. However,
if the Determine Range procedure in this section is
performed, it should be performed before mounting the
gripper fingers.

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Genesis Setup and Service Software Section 11

Adjust Rotator
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Perform the following steps to adjust the rotator’s offset and


scaling.
1. On the Contents page, select the Adjust Rotator
checkbox (聺) and switch to the Setup page (Figure
11.67).

Figure 11.67:RoMa Panel - Setup Page

2. Use the Reference Plate Position spin box to set the


position of the test plate (front left corner) to grid 25.
3. Install the procedure by selecting Start. The message
Mount Gripper displays.
4. Mount the gripper fingers if they were removed and
confirm with OK. The message Make sure the reference
plate is on Grid 25 displays.
5. Put the test plate on the worktable at the selected grid
position (front left corner) and continue with OK. The
rotator is now adjusted and the values found are written to
the RoMa. Select OK when the test is completed.
6. Verify the setup by performing the Reference Plate test.
7. Select Close to exit the RoMa panel.

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Section 11 Robotic Manipulator Arm (RoMa)

Information Page

Purpose
The Information page summarizes device parameters and
diagnostics data.

Figure 11.68: Information Page

Controls, Data
The Information page includes the following controls.
Control Description
Refresh Reloads diagnostic data and refreshes the
Sample Time.
Sample Time Snap shot time, e.g., time when diagnostic
data were read.
Device Parameters
Axes Axis for which the test was done.
Offset Offset, in mm, for each axis.
Displ Displacement, in mm, for each axis.
Range Range, in mm, for each axis.
Scale Scale factor.
Accel Acceleration, in mm per square sec., for
each axis.
Speed Normal speed, in mm per sec., for each axis.

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Genesis Setup and Service Software Section 11

Control Description
Move Speed Speed, in mm per sec., at which the RoMa
was actually moved in each axis.
Diagnostics Data
Axis Axis in which the RoMa was moved.
Moves Number of moves per axis.
Moves Number of clearable moves per axis.
Distance Total moved distance, in meters, per axis.
No-Loads Total number of no-loads, e.g., axis crashed.
Status Status of the axis in question (ready,
initialization error, not implemented, not
initialized, not loaded, unknown).

Viewing the Data


Perform the following steps to access the Information page.
1. Switch to the Information page (Figure 11.51).
2. Select Refresh. View the data.
3. Print the setup results with Print or preview the Test
report (Printing, page 11-146).

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Section 11 Robotic Manipulator Arm (RoMa)

Printing

Purpose
The Printing page allows formatting of a Test report to meet
laboratory requirements.

Figure 11.69: RoMa Panel - Printing Page

Controls
The Printing page includes the following controls.

Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Device Checkbox to select device data.
Diagnostics Checkbox to select diagnostic data as
shown on the Information page (Figure
11.51).
Configuratio Checkbox to select the configuration
n data (address, firmware version,
bootware version, Z-range, orientation,
gripper type).
Parameters Checkbox to select device parameters as
shown on the Results page (Figure
11.51).

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Genesis Setup and Service Software Section 11

Control Description
Multiple existing Data selection valid for all device data
Sections selected in the Detailed Print Section
Configuration section.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to
setup a printer.

Printing a Report
Perform the following steps to print a report.
1. Switch to the Printing page (Figure 11.53).
2. Select the data to print in the Detailed Print Section
Configuration section.
3. Preview the Test report onscreen with Result Preview.
4. Print the Test report with Print.

Test Report
Based on the selections, the Test report includes the following
data.

Test Report
Header
Global data such as software versions, RoMa and
instrument serial numbers, date of creation, and global test
review (not relevant). Signature section with operator
name.
Device
Diagnostics: Same table as displayed on the Information
page (refer to Figure 11.51).
Configuration: Address, firmware version, bootware
version, Z-range, orientation, gripper type.
Parameters: Same table as on the Results page (refer to
Figure 11.51).

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Section 11 Robotic Manipulator Arm (RoMa)

Test Report
Setup Results
Deviation: Shows the deviations of the arm for movements
in X- and Y-directiona
a. Deviations in X- and Y-direction
Y-deviation: is 0 if the arm moves exactly parallel to the
worktable in the X-axis.
X-deviation: is 0 in the Y-axis if the arm moves exactly at a
right angle (90°) to the worktable in the Y-axis.

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Genesis Setup and Service Software Section 11

Liquid System Functions


Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.

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Section 11 Liquid System Menu

Liquid System Menu


The Liquid System menu includes the following commands.

Command Description
DiTi DiTi (disposable tips) functions; refer to
DiTi, page 11-176.
Liquid Liquid detection functions; refer to DiTi,
Detection page 11-176.
Gravimetric Checks the precision and accuracy of a
Test liquid transfer. Not used with the m2000sp
instrument.
FaWa FaWa functions; refer to DiTi, page 11-176.
6-way valve Checks if the 6-way valve works correctly
and if the valve and its connector are
completely tight. Not used with the
m2000sp instrument.
Licos MPO Licos on MPO functions. Not used with the
m2000sp instrument.
Licos Licos on supervisor PCB functions. Not used
(Supervisor) with the m2000sp instrument.
Balance Sets up balance. Not used with the m2000sp
instrument.

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Genesis Setup and Service Software Section 11

DiTi
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.

DiTi Sub-menu
The DiTi sub-menu includes the following commands.

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Section 11 DiTi

Command Description
DiTi Eject Test Checks the positioning accuracy of the DiTis
and DiTi ejection procedure.
Lower DiTi Tests the put back and disposal ejection
Eject Test heights for the Lower DiTi eject.

Requirements
To execute the Lower DiTi Eject test, the m2000sp worktable
map must be loaded and tips currently in use must be
configured.

Loading a Worktable
Perform the following step to load a worktable.
Load the suitable worktable map with <System><Load
Worktable>.

Figure 11.70:m2000sp Worktable

Lower DiTi Eject Test


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Lower DiTi Eject Test function tests the putback and
disposal ejection heights for the Lower DiTi Eject. The
calibration function must be performed before the Lower DiTi
Eject test is used.

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Genesis Setup and Service Software Section 11

Before Starting the Lower DiTi Eject Test


• Ensure requirements at the beginning of DiTi, page 11-176
are met.
• Ensure the Calibrate Lower DiTi option was executed.

Procedure
Perform the following steps for the Lower DiTi Eject test.
1. Go to <Liquid System><DiTi><Lower DiTi Eject Test>.
2. Load the m2000sp worktable:
• Place the DiTi Rack carrier at grid 21.
• Place the wash/waste station and DiTi slide in the
cutout at grid 18.
• Place a full rack of 1000 µL DiTis on the rear position
of the carrier.
• Place an empty rack on the center position of the
carrier.

Figure 11.71:Lower DiTi Eject Test Dialog

3. The Lower DiTi Eject Test screen displays.


4. Configure the Lower DiTi Eject Test as shown:

NAT MBS m2000sp


DiTi Type 1000F EDiTi 1000F EDiTi
Source Rack DiTi.RR C12.RR
First DiTi Position 1 1
Destination Rack DiTi.RM C12.RM
Waste Rack W1.DiTi Waste C11.DiTi Waste

For both setups:


• Leave <Put back DiTi on lower position> blank
• Enter the operator name (required)

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Section 11 DiTi

5. Click Select Tips and verify all eight DiTi tips are 1000 µL
DiTi with filter.

Figure 11.72:Select Tips

6. Select OK.
7. Initiate the test by selecting Run. The status changes to
Test Running.
8. When the test is completed, the status changes to Test
Passed.

9. Select Close to finish the test.


10. If the test failed:
• Verify the LiHa reference positions.
• Check the Tip adapters.
11. Remove the carrier, DiTi Slide, and wash/waste station
from the worktable.
12. If the test failed:

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Genesis Setup and Service Software Section 11

• Verify the LiHa reference positions.


• Check the Tip adapters.
• Perform the Individual Z Alignment
• Calibrate the Lower DiTi Eject.

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Section 11 Liquid Detection

Liquid Detection
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
WARNING: Pinch Point. The instrument requires
accurate positioning of system modules, and carriers
and racks on worktables. When operating the
instrument, it could be necessary to run tests with the
instrument’s safety panels open.
Keep body parts away from moving parts of the
instrument (e.g., LiHa, RoMa, tips, and gripper fingers)
during setup and test procedures.
• Stand back from the worktable surface during
these procedures.
• Fast moving devices could cause injuries.
The Liquid Detection sub-menu includes the following.

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Genesis Setup and Service Software Section 11

Command Description
Setup Defines new Liquid Level Detection setups
for user-defined tests.
Normal Test Ensures the Liquid Detection feature works
correctly.
Extended Test This sub-item can only be performed at
manufacturer’s site and is disabled for use by
operators and Field Service Representatives.

Setup
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Setup function defines Liquid Level Detection (LLD)
setups to use with user-defined tests. The LLD setup is not used
by Tecan’s application software such as Gemini and Logic.
Because most liquids used have different capacitive dielectric
properties, the relevant parameters are defined within the
(LLD) Setup. Tests within Setup & Service can use one of
10 pre-defined setups (range 1 to 10) or one of 20 user-defined
setups (range 11 to 30). Each setup defines the LLD procedure,
sensitivity, phase, and In and Out parameters.

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Section 11 Liquid Detection

Procedure
Load the m2000sp Worktable and perform the following steps
to define LLD setups.
1. Start the function with <Liquid System><Liquid
Detection><Setup>. The Liquid Detection Setups
window displays.

Figure 11.73:Liquid Detection Setups Window

The Liquid Detection Setups window is divided into the


following sections.

Command Description
Default setups Ten pre-defined LLD setups.
These settings (refer to Table 11-1) cannot be
altered.
User-defined Twenty user-defined LLD setups.
setups
Proc Number of the liquid detection procedure. The
procedure that the liquid detection follows describes
the various steps executed by the LLD.
Sens Detection sensitivity of the liquid detector electronics.
NOTE: Liquids of low conductivity require very
high detection sensitivity (2), and vice versa.
0 Low or standard sensitivity
1 High sensitivity
2 Very high sensitivity

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Genesis Setup and Service Software Section 11

Command Description
Phase This feature is provided because the detection signal of
a tip can interfere with the check function of adjacent
tips at a very high sensitivity level. This especially can
occur if the latter tips enter the same liquid
simultaneously (e.g., in an additive trough rack).
0 0° (in phase). Causes the detector electronics to
check all tips involved in the detection
simultaneously.
1 180° (out of phase). Not used with the m2000sp
instrument.
Causes odd tip numbers to be checked a
fraction of a second later than even tip
numbers.
In Flag to enable the liquid entry signal monitoring
(liquid level detection).
Yes Enabled
No Disabled
Out Flag to enable the liquid exit signal monitoring (clot
detection).
Yes Enabled
No Disabled
Use Trough Checkbox to activate a special liquid detection
Detection Mode mode for containers simultaneously accessed
by several tips.
Close Finishes the function.
Save Saves the LLD setups in the LLDSETUP.ini file in the
<install_path>\Service directory.
Print Opens the Common Print Information dialog to
print the LLD setups.

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Section 11 Liquid Detection

2. Define the LLD setup within the User-defined setups


table.
• Select Use Trough Detection Mode checkbox (聺) to
activate a special liquid detection mode for containers
simultaneously accessed by several tips. If
selected—when encountering such a container—the
first tip moves down to detect liquid, registers the
liquid level, then all other activated tips move down
to the same liquid level. Different tips are considered,
according to the tip configuration.
• For the m2000sp instrument, Phase must be set to 0.
3. Save data with Save. A message displays that setup data are
stored in the Toolbox and Liquid Detection Setup file.
4. Confirm with OK to finish.

Liquid Detection Procedures


The following table defines the various test procedures.

Double
Proc Description
Detection
Detects with all selected tips
0 no
simultaneously.
Detects with all selected tips
simultaneously. Stores the position of
the first successful detection, retracts
then redetects. The second detection
1 yes
must be within ± 50% of the retract
distance—if not, detection is restarted.
After four unsuccessful detection
attempts an error message displays.
Detects simultaneously with all odd
(1,3,5,7) of the selected tips, and
2 no afterward with all even (2,4,6,8) of the
selected tips.
Otherwise, same as procedure #0.
Detects simultaneously with all odd
(1,3,5,7) of the selected tips, and
3 yes afterward with all even (2,4,6,8) of the
selected tips.
Otherwise, same as procedure #1.

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Double
Proc Description
Detection
Tip after tip until all selected tips are
4 no through.
Otherwise, same as procedure #0.
Tip after tip until all selected tips are
5 yes through.
Otherwise, same as procedure #1.
Tip after tip with 80 ms delay in
between, until all selected tips are
6 no through.
Otherwise, same as procedure #0.
Tip #1 moves down, detects, then
7 yes retracts 10 mm. The next tip then
moves down.

Double Detection
no Only one detection.
yes Double detection for increased accuracy
(recommended by Tecan).

Genesis Default Liquid Level Detection Setups

Tab. 11-1 Genesis Default LLD Series

LLD Double Container


Conductivity Proc Sens Phase In Out
Setup Detection Form
1 no Tube like high 2 0 0 yes yes
2 no Tube like low 2 2 0 yes yes
3 no Tube like medium 2 1 0 yes yes
4 no Well like all liquids 6 2 0 yes yes
5 yes Tube like high 3 0 0 yes yes
6 yes Tube like low 3 2 0 yes yes
7 yes Tube like medium 3 1 0 yes yes
8 yes Trough all liquids 7 2 0 yes yes
like
9 0 0 0 yes yes
10 0 0 0 yes yes

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Section 11 Liquid Detection

Normal Test
Description
Container Form
Tube like Tube is a strip rack or any other rack with
diameter greater than 8 mm.
Well like Microplate well or any small tube with
diameter smaller than 8 mm.
Trough like Reagent bottle or trough accessed by more
than 1 tip.
Conductivity Conductivity of the detected liquid.
Non-conductive liquids, e.g., oil, are not
detected.
NOTE: Liquids of low conductivity
require very high detection sensitivity
(Sens), and vice versa.
low Liquid of low conductivity, e.g., DMSO,
alcohol, deionized water.
medium Liquid of high or medium conductivity, e.g.,
blood, salty water, deionized water.
high Liquid of high conductivity, e.g., blood,
salty water.
all liquids Liquid of high, medium or low
conductivity, e.g., blood, salty water,
deionized water, DMSO, alcohol.

Purpose
The Normal Test function verifies the Liquid Detection feature
is working properly. It is also frequently used to check if Liquid
Detection is able to detect a certain (usually small) volume of a
specific liquid.
In addition, there is an Extended test, which is performed
before the instrument is shipped. It cannot be executed in the
field and is not described here.
NOTE: Tubing should be free of all bubbles. Perform
the Fill/Flush test to remove bubbles.

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Procedure
Perform the following steps for the Normal Test function.
1. Load the m2000sp worktable map with <System><Load
Worktable>.
NOTE: If you have loaded the m2000sp worktable, you
must run the test with 200 mL reagent carriers and
troughs.
2. Fill a clear reagent trough with 20 to 30 mL of water. Place
the trough into the first position (RF) of a reagent carrier
and load the carrier on grid 20 of the worktable.
3. Place a full rack of 1000 µL DiTis in the rear position (RR)
of a DiTi carrier and place the carrier on grid 21 of the
worktable.
4. Place a rack with eight clean and dry tubes in positions
1 to 8 on grid 1 of the worktable.
5. Select <Liquid System><Detection Mode> option. Select
Save. Select OK. Select Close.
6. Start with <Liquid System><Liquid Detection><Normal
Test>. The Liquid Detector Test Configuration dialog
displays.

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Section 11 Liquid Detection

7. The Liquid Detector Test Configuration dialog includes


the following controls.

Control Description
Cycles Field for the number of cycles performed.
Enter 15.
Submerge Field for the distance the tip dips into the
liquid. Enter 10.
Is the Liquid Checkbox to define that the liquid source is
Source a Rack? a rack. Select this option.
Source Rack List to select the position of the rack
holding container(s) out of which liquid is
aspirated. Use T1.RF.
Flush Rack List to select the position of the flush carrier
within the wash rack. Select W1.Waste,RM.
Destination List to select the position of the destination
Rack rack. For non-NPS instruments, ensure the
tubes of C4.R1 are empty.
First Dest Field for the destination start position.
Position Position 1 is the upper tube. Enter 1.
Diti Waste List to select the position of the waste
Rack carrier within the DiTi rack. Select
W1.DiTi.Waste.
DiTi Type List to select tip type. Select 1000F EDiTi.
DiTi Tip type.
Diti Rack List to select the position of the DiTi carrier
within the DiTi rack. Select DiTi.RR.
First DiTi Field for the DiTi start position. Position 1
Position is the first one in the upper left. Enter 1.
Run Opens an empty Liquid Detector Test Results
window (Figure 11.74) to start the test
procedure.
Close Finishes the function.
Cancel Aborts the function and resets all values.
Save Starts the standard Windows Save As dialog to
save the data in an appropriate *.ini file in the
<install_path>\Service directory.
Print Opens the Common Print Information dialog
to print configuration data and/or test results.

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Control Description
More Opens the Liquid Detector Test—Advanced
Configuration dialog for advanced
configuration (refer to Advanced Configuration
on page 11–192). For FSEs only and requires the
appropriate password.
Select Tips Opens the Select Tips dialog to select the tips
used.
Fill/Flush Opens the Fill/Flush dialog to fill/flush the
liquid dispense system.

8. Set the appropriate test configuration according to the


worktable map.
9. If definition of an advanced configuration is needed,
continue with Advanced Configuration.
10. If tips used for the test need to be reduced, click Select Tips
then make the selection.
11. Start the test with Run. An empty Liquid Detector Test
Results window (Figure 11.74) displays.
12. Start the test procedure with Start. The message Do flush
Step? (First cycle with all tips) displays.
13. Confirm with Yes or No. If confirmed with Yes, the
message Repeat flush step? displays. If No is selected, the
test starts running.
14. During the test, results display continuously on the
Liquid Detector Test Results window as shown.

Figure 11.74:Liquid Detector Test Results Window

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Section 11 Liquid Detection

The Liquid Detector Test Results window includes the


following data.

Description
Tip-in Results when tip enters liquid.
CurrLevel Current Z-value, in 1/10 mm (measured
from bottom).
MeanLevel Average of all detected liquid levels.
Min-Max Largest difference, in 1/10 mm, between
min. and max. level.
LiqErrs The value is incremented if:
• no liquid is detected when the tip dips
into the liquid (no dip in signal
• no dip out signal is detected double
detection mode)
Level Err Set to yes. If the no dip in signal received
within the required distance, e.g., if the
allowed Liquid Det. Error Limit (Advanced
Configuration) is exceeded.
Tip-out Results when tip leaves liquid.
MeanLevel Average of all detected liquid levels.
ClotErrs Numbered errors, if no dip out signal
received within retract distance, e.g., if the
allowed Clot Det. Error Limit is exceeded. A
dip out signal occurs as the tip leaves the
liquid.
No Exit Numbered errors, if no dip out signal
received.
TotErrs Sum of all errors.

15. After finishing the test, the message Liquid Detector Test
passed or Liquid Detector Test failed displays. Confirm
with OK. The Liquid Detector Test Results window with
the final test results is active.
16. Close the window with Close. The Liquid Detector Test
Configuration dialog is active again.
17. Print the test results with Print and/or finish the test with
Close.

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Pass/Fail Criteria
The test passes if TotErrs = 0.
If the test fails:
• Verify all tips are the same height. If not, correct with
<LiHa><Setup><Individual Z>.
• Recheck the liquid system for air bubbles.

Advanced Configuration
Field service representatives can use an advanced test
configuration.
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Procedure
Perform the following steps to enter Advanced Configuration
data.
1. Select More in the Liquid Detector Test Configuration
dialog. The Liquid Detection Test - Advanced
Configuration dialog displays.

Figure 11.75:Liquid Detection Test - Advanced


Configuration Dialog

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Section 11 Liquid Detection

The Liquid Detection Test - Advanced Configuration


dialog includes the following controls.
Control Description
Liquid
Detection
LLD Setup Fields to define the LLD Setup number
(refer to Setup, page 11-182) for dip in
(Liquid) and dip out (Clot).
Detect Fields to define the detect speeds, in
Speed 0.1 mm/s, for dip in (Liquid) and dip
out (Clot).
Det. Error Fields to define allowed deviations, in
Limit 0.1 mm, to not lead to LiqErrs
(Liquid) or ClotErrs (Clot).
Prefill Prefill parameters (used only for
non-NPS instruments).
LLD Setup Field to define the LLD Setup number
used for prefill.
Volume Field to define the prefill volume, in
mL.
Speed Field to define the dispense speed of
the liquid, in mL/s.
Air Gap Field to define the air volume between
system liquid and prefill liquid.
Tip Usage List to select the tips used.
OK Finishes the advanced configuration and
saves the values defined.
Cancel Aborts the advanced configuration without
saving any changes.

2. Enter advanced configuration data.


3. Confirm entries with OK. The Liquid Detector Test
Configuration dialog is active again (back to Procedure).

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Fill/Flush
1. From the Liquid Detector Test Configuration screen
select Fill/Flush.

2. The Fill Liquid System screen displays.

3. Select the Use Fast Wash Pump checkbox (聺).


4. Depending on the worktable you are in, select
W1.WASTE,RM or C11.Waste,RM in the Flush Station
list.
5. Select W1.DiTiWASTE or C11.DiTiWaste in the DiTi
Waste Rack field.
6. Set the System Liquid Aspirate Speed to 1000 µL/s.
7. Set the Fill Speed to 1000 µL/s.
8. Set the Fill Liquid Volume to 30000 µL.
9. Select Fill System to initiate the Fill/Flush protocol.

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Section 11 FaWa

FaWa
Observe the following safety notices.
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
The Fast Wash pump (FaWa) is used to pump large amounts of
liquid at high speed through the system, normally to rinse the
tips. The Monitored Pump option (MPO) consists of FaWa and
Licos. The m2000sp instrument only uses the FaWa pump and
the common board for the MPO, and does not use the Licos
system.

The FaWa sub-menu includes the following commands.

Command Description
Setup Configures the Fast Wash pump; refer to Setup,
page 11-196.
Test Checks the throughput of liquid in the entire
Genesis liquid system tubing; refer to Test,
page 11-196.

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The FaWa command is enabled only if a Fast Wash pump is


installed.

Setup
Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Setup function configures the Fast Wash pump (number
of pumps and tips for each pump).

Procedure
Perform the following steps to configure the Fast Wash pump.
1. Start the function with <Liquid System><FaWa><Setup>.
The Fast Wash Pump Setup dialog displays.

Figure 11.76:Fast Wash Pump Setup

2. In the Number of Pumps list select the number of pumps.


There is only one Fast Wash pump installed on the
m2000sp instrument.
3. In the Pump1 list, select 8 as the number of tips served by
the installed pump.
4. Confirm entries with OK.
5. If any data were changed, the message Write results to
MPO EEPROM displays. Confirm with Yes.

Test

Purpose
The Test function checks the throughput of liquid in the
entire m2000sp liquid system tubing.

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Section 11 FaWa

If the tip type is not recognized, the operator is prompted to


enter user-defined values as shown in the following table.

Test Principle
The following figure illustrates the principle of the liquid level
check with the Fast Wash Pump test.

8-Tip Instruments
Tip type, built-in Duration of Min. throughput
configurations pump… to pass test (µL/s)
(0.1 s)
1000 µL DiTi 30 1600
200 µL DiTi 30 1600

Liquid Level
after second
liquid burst

Cylindrical part Prefilled


of liquid level liquid, first
(volume liquid burst
500 mL)

Figure 11.77: Liquid Level Check

Test Sequence
The Fast Wash Pump test performs the following procedure to
test the throughput of the pump through the tips or DiTi
adapters.
• Tubes are pre-filled, using Dilutor and without DiTis if the
instrument has DiTi adapters.
• DiTis are picked up.
• DiTi actions only take place if the selected tips are a DiTi
type.
• Level of the pre-fill is measured, using Liquid Detection.
• DiTis are removed.

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Genesis Setup and Service Software Section 11

• Tubes are filled using the Fast Wash pump.


• DiTis are picked up.
• The height increase of liquid is measured using Liquid
Detection.
• The height increase is checked; it must be within the
expected minimum throughput value.

Before Starting the FaWa Test Function


• To avoid overflow of liquid during the test, use only tubes
with a 13 mm diameter and at least 100 mm tube length.
Ensure tubes are empty and clean.
• Ensure no other Liquid System Check Worktable map was
loaded; if not load it with <System><Load Worktable>.
• Verify the system liquid bottle is full and at the same
height as the worktable.
• The liquid system must be filled with <FaWa><Test><Fill
Flush>. Ensure the entire liquid system is free of air
bubbles.

Procedure
1. Start with <Liquid System><FaWa><Test>. The Fast
Wash Pump Test Configuration dialog displays.

Figure 11.78:Fast Wash Pump Test Configuration

2. If no worktable map was previously loaded, the Select


Worktable dialog displays to load an appropriate
worktable map. Select m2000sp Worktable and select OK
to load.

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Section 11 FaWa

The Fast Wash Pump Test Configuration dialog includes


the following controls.

Control Description
Prefill Field for prefill volume in µL. Set the value
Volume so the liquid level reaches the cylindric
part of the destination tube. Required for a
proper liquid level check. The default
setting is 600.
Destination List to select the position of the
Rack destination liquid rack. Select C3.R1 at
grid 4.
Flush Rack List to select the position of the flush
carrier within the wash rack. Select
W1.WASTE,RM.
DiTi List to select the type of DiTi used. Select
Type 1000F EDiTi.
DiTi Rack List to select the position of the DiTi
carrier within the DiTi rack. Select
DiTi.RR. Place the carrier at grid 21.
DiTi Waste List to select the position of the waste
Rack carrier within the DiTi rack. Select
W1.DiTi Waste.
First DiTi Field for the DiTi start position. Position 1
position is the first one in the upper left. Enter 1.
Select Tips Opens the Select Tips dialog to select the
tips used.
More Opens the Fast Wash Pump Test - Adv.
Configuration dialog for user-defined test
configuration.
Fill/Flush Opens the Fill Liquid System dialog.
Run Opens an empty test result window
(Figure 11.79) to start the Fast Wash Pump
test.
Close Finishes the function and saves the values
defined.
Cancel Aborts the function without saving any
changes.

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Control Description
Save As Starts the standard Windows Save As
dialog to save the data in an appropriate
*.ini file in the
<install_path>\Service directory.
[Print] Opens the Common Print Information
dialog to print the configuration data
and/or test results. Refer to Printing, page
11-135 for details.

3. Set the appropriate test configuration according to the


worktable map.
4. To define a user-defined test, continue with Advanced
Configuration by selecting More. Refer to Advanced
Configuration, page 11-201 below.
5. Start the test with Run. An empty Fast Wash Pump Test
Results window (Figure 11.79) displays.

Figure 11.79:Fast Wash Pump Test Results

6. Start the test procedure with Start. The test begins


running.
7. After finishing the test, the message Fast wash pump test
passed or Fast Wash Pump Test failed displays. Confirm
with OK. The Fast Wash Pump Test Results window with
the final test results is active.
8. Close the window with Close. The Fast Wash Pump Test
Configuration dialog is active again.
9. Print the test results with Print and/or finish the function
with Cancel.

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Section 11 FaWa

Pass/Fail Criteria
The test passed when the Throughput value is > the minimal
throughput value displayed in brackets in the Tip column. If
Throughput is < the minimal throughput value (in the
example < 1600 µL), the test failed.
The minimal throughput values are user-defined within the
Fast Wash Pump Test - Adv. Configuration dialog (in the
example > 1600 µL).

If Test Fails
1. Verify tips are not blocked.
2. Recheck the liquid system for air bubbles.

Advanced Configuration
Perform the following steps to enter user-defined test data.
1. Select More in the Fast Wash Pump Test Configuration
dialog. The Fast Wash Pump Test - Adv. Configuration
dialog (Figure 11.80) displays.

Figure 11.80:Fast Wash Pump Test - Adv.


Configuration Dialog

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Genesis Setup and Service Software Section 11

The Fast Wash Pump Test - Adv. Configuration dialog


includes the following controls.

Control Description
6-way valve Indicates if a 6-way valve is installed. Not used
installed with the m2000sp instrument.
LLD Setup Field for the LLD Setup number; refer to Setup,
page 11-182. The LLD Setup should not be
altered.
User-defined
.. user-defined Checkbox to activate the user-defined test.
test
Name of … Field for a test configuration name, e.g., FaWa.
Duration Field for the duration of the pump sequence,
in 0.1 s.
If changing the duration to a higher value, be
sure to place an appropriate tube with a bigger
volume, e.g., 30, in the destination rack to
avoid an overflow.
Minimal Field to define the minimal expected
Throughput throughput, in µL/s, to pass the test (usually
1600).
Test settings Displays the current test settings.
Select Tip Type Displays the current selected tip, e.g., 1000 µL
DiTi with filter.
Number of Used Specifies the number of tips used.
Tips
Duration Duration of the test.
Minimal Set fill rate to pass test.
throughput to pass
Test
OK Finishes Advanced Configuration and saves the defined
values.
Cancel Aborts Advanced Configuration without saving any
changes.

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Section 11 FaWa

2. To reduce the number of tips used for the test, click Select
Tips. The Select Tips dialog displays.

Figure 11.81:Select Tips Dialog

3. When using a custom-defined tip configuration, select the


positions accordingly. Confirm entries with OK.
If a selection is made, the message This is not one of the
standard configurations displays:

Figure 11.82:FaWa Test

4. Confirm entries with OK. One of the following messages


display: Your user-defined configuration is accepted.
FaWa-Test will run or Program chosen default
configuration. FaWa-Test will run.
5. Confirm with OK. The Fast Wash Pump Test
Configuration dialog is active again (back to Fast Wash
Pump Test - Adv. Configuration Dialog).
6. Start the test with Run then Start. The test proceeds as
previously described.

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Genesis Setup and Service Software Section 11

Fill/Flush
1. Load the m2000sp Worktable if not loaded.
2. Select <Liquid System><FaWa> Test from the menu.
The Fast Wash Pump Test Configuration screen displays.

3. Select 1000F EDiTi as the DiTi Type.


4. Select Fill/Flush. The Fill Liquid System screen displays.
5. Check the Use Fast Wash Pump checkbox (聺).

6. Select W1.WASTE, RM from the pull down menu for the


Flush Station, and W1.DiTiWaste for the DiTi Waste
Rack.
7. Set the System Liquid Aspirate Speed to 1000 µL/s.
8. Set the Fill Speed to 1000 µL/s.
9. Set the Fill Liquid Volume to 30000 µL/s.
10. Select Fill System to initiate filling of the dispense system.
Elimination of bubbles:
11. To run this procedure to eliminate bubbles from the
dispense tubing and syringes, uncheck the Use Fast Wash
Pump checkbox (□), reduce the Fill Liquid Volume from
30000 to 3000, then select Fill System.

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Section 11 FaWa

12. When the test is running, tap the liquid lines to dislodge
any bubbles lodged in the dispense tubing network.

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Genesis Setup and Service Software Section 11

Options Menu
Menu Items
The following menu displays items that can be included in the
Options menu.

Figure 14.1: Options Menu

Items included in the Options menu are described in this


section.

Commands
The Options menu includes the following commands.

Command Description
Easy Option Uninstalls an easy option. Not used with the
m2000sp instrument.
Heated Heated incubator functions. Not used with
Incubator the m2000sp instrument.
Plate Washer Plate washer functions. Not used with the
m2000sp instrument.
Access Status Checks door locks functionality.
Options
Incubation Calibrates and checks the 1 mL Subsystem.
Device
SMIO for NAT Calibrates and checks the system liquid and
waste sensors.

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Section 11 Access Status Options

Access Status Options

The Access Status Option function tests Access and Status


options such as the main door locks. The m2000sp instrument
does not use alarm devices or the pause/resume button.

Door Lock V2

Figure 14.2: Door Lock

Files, Directories
The Access Status Option function creates the following files
(refer also to Setup & Service, page 11-10).

Description
Result Files Directory: <install_path>\Results
File name: AccessStatusOption_<serial
_no>_<date>_<time>.any
Test Directory user-defined
Configuration File name <name>.any (Enter the full file
Files name (with extension .any)

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Access Status Options Panel

Pages
The Access/Status Panel window includes the standard
command buttons and is subdivided into the following pages.

Page Description
Contents General overview, device, and procedure
selection.
Configuration Test configuration settings and tool buttons.
& Tools
Printing Print selection for the Test report; refer to
Printing, page 11-215.

Controls
The Contents page includes the following controls.
Control Description
Available List of all devices to which Access and Status
Devices options can be connected.
Genesis-CU Front door locks connected to the CU
board.
Access Status Door locks, alarm device V3, and/or
Pause/Resume button connected to the
Access Status Options board.
Setup Setup procedure for supervisor board. Not
used with the m2000sp instrument.
Set Defaults Sets Tecan defaults; refer to EEPROM,
in EEPROM page 11-42.

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Section 11 Access Status Options

Control Description
Test Test procedure.
Device Test Tests Access and Status options.

Using the Panel


Perform the following steps to use the Access Status Option
panel.
1. Start with Options  Access Status Options. The
Access/Status Panel window with activate Contents page
displays.
2. In the Available Devices section, select the device to test
to which Access and Status option(s) are connected.
Continue as described in the appropriate section.
NOTE: If another device is selected, current test
results are deleted.
3. Before starting any test procedure, switch to the Printing
page to complete the Operator and Comment fields.
4. Start the desired procedure with Start.
5. Print the test results with Print or switch to the Printing
page to preview the Test report.
6. Finish with Close.

Setup: Set Defaults in EEPROM


Password Protection: This procedure is exclusively for
FSEs and requires use of the appropriate password.

Purpose
The Set Defaults in EEPROM procedure sets internal
configuration data of Access Status options connected to the
SMIO-SAFY board to Tecan defaults. This must be done once if
a new software version is installed.
NOTE: This procedure is only enabled and executable if
the SMIO-SAFY board is selected in the Available
Devices section.

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Genesis Setup and Service Software Section 11

Procedure
Perform the following steps to set the Tecan defaults.
1. On the Contents page, select the following:
• SMIO-SAFY entry in the Available Device section.
• Select the Set Defaults in EEPROM checkbox (聺). No
further parameters need definition.
2. Download the default values to the SMIO-SAFY board and
select Start to have them reread by the Setup & Service
software.
3. Confirm the message Setup: Done with OK.

Test: Device Test


WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.

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Section 11 Access Status Options

Procedure
Perform the following procedure to test the front door locks.
1. Select Access Status Options from the Options menu.

2. The Access/Status Panel displays. Select Access Status


under Available Devices. Select the Device Test under
Test.

3. Select the Configuration & Tools tab from the menu.


Select 'Door Lock (left) and Door Lock 2 (right) from the
Configuration menu. Select Apply to save the
configuration.
4. Check the door lock function of the desired lock by
selecting Lock then Unlock. Troubleshoot the door lock if
it does not respond to the command with an audible click.

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5. Select Start to formally check the door lock function.

6. The prompt Please close the main door for Door Lock 1
(left) displays. Select OK.

7. The prompt Check if the left side is locked now displays.


Select OK if successful, if not select Cancel. If Cancel is
selected because the lock is not functioning, troubleshoot
the lock. Select OK.

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Section 11 Access Status Options

8. The prompt Check if it is unlocked now displays. Select


OK if successful, if not select Cancel. If Cancel is selected
because the lock is not functioning, troubleshoot the lock.
Select OK.

9. The prompt Please close the main door for 'Door Lock 2
(right)' displays. Select OK.

10. The prompt Check if the right side is locked now


displays. Select OK if successful, if not select Cancel. If
Cancel is selected because the lock is not functioning,
troubleshoot the lock. Select OK.

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Genesis Setup and Service Software Section 11

11. The prompt 'Check if it is unlocked now displays. Select


OK if successful, if not select Cancel. If Cancel is selected
because the lock is not functioning, troubleshoot the lock.

12. Select OK if successful, if not select Cancel. If Cancel is


selected because the lock is not functioning, the test fails.
Troubleshoot the lock. The operator is notified the test
passed.

13. Select OK to end the test. The prompt Access Panel


displays.

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Section 11 Access Status Options

Printing
The Printing page allows formatting of a Test report to meet
laboratory requirements. It can be viewed onscreen and
printed.
1. Switch to the Printing page.

The Printing page includes the following controls.

Control Description
Detailed Print Section Configuration
Header Checkbox to select global data.
Device Checkbox to select device data.
Operator Field for operator name. An entry is
recommended.
Comment Field for additional notes. An entry is
recommended.
Result Preview Opens a Test report preview window.
Printer Setup Starts the standard Windows dialog to set up
a printer.

2. Select the data to print in the Detailed Print Section


Configuration section.
3. Preview the Test report (refer to Test Report, page 11-216)
onscreen with Result Preview.
4. The Test report can be printed with Print.
NOTE: If another device is selected, the current Test
report is deleted.

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Genesis Setup and Service Software Section 11

Test Report
Depending on the selection, the Test report includes the
following data.

Test Report
Header
Global data such as software versions, device and
instrument serial numbers, date of creation, and global
test review such as:
Test Result: Passed, if all tests passed, otherwise Failed
Tests done: All or Not all
Test Configuration: not relevant
Device Default
Settings: not relevant
Signature section with operator name
Device
Configuration: firmware version and bootware version
Access/Status Test: Passed or Failed or Not Done
Detailed Results
Depending on the tests performed, the following
results are printed:
Door Lock 1 (left): Passed or Failed
Door Lock 2 (right): Passed or Failed
Door Lock 3 (aux): Passed or Failed
Door Lock 4 (aux): Passed or Failed
RT-Incubator 1 (right) Front Door: Passed or Failed
RT-Incubator 1 (right) Slots: Passed or Failed
RT-Incubator 2 (left) Front Door: Passed or Failed
RT-Incubator 2 (left) Slots: Passed or Failed
Alarm Device: Passed or Failed
Alarm Green: Passed or Failed
Alarm Red: Passed or Failed
Pause/Release Button: Passed or Failed

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Section 11 Incubation Device

Incubation Device
WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.
CAUTION: Hot Surface. Identifies an area where the
FSE could be exposed to hot surfaces, such as heater
zones 1 and 2. Allow the area to cool before servicing.
NOTE: This section can only be used with a TEMPO
110 measuring device. Refer to Section 9, Repair,
Replacement of Spare Parts, for an alternate method
for checking heater block temperature.
The Incubation Device panel provides for setup, installation,
and verification of the heating plates that are part of the 1 mL
Subsystem.

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Genesis Setup and Service Software Section 11

Setup
1. From the Main menu, select Options  Incubation
Device. The Incubation Device panel displays.

2. Select the device to set up and highlight the device in the


Available Devices: window.
3. Select the Install Plates/Create or Edit Custom Plates
checkbox (聺) under Setup:.
4. Two additional tabs, Plate Installation and Setup
Settings are available.

5. Select the Plate Installation tab. The following page


displays.

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Section 11 Incubation Device

6. From the Available Plates dialog of the Plate Installation


tab, select the NAT 50/75 plate.
7. Use the right arrow key () to move the NAT 50/75 plate
from the Available Plates: window to the Installed
Plates: window.
8. Select the TEMPO110 option under the Heating Block
Calibration by. menu.
9. Set the Lower temperature: to 50°C and the Upper
temperature: to 75°C.
10. Select Apply to apply the settings.
11. Select Start to initiate the protocol. The operator is
prompted to connect the TEMPO110 measuring device to
the instrument.
12. The operator is notified the procedure is proceeding.
13. On completion of the test, the operator is notified if the
procedure passed or failed.
14. Select OK.

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Genesis Setup and Service Software Section 11

Burn-In of Heating Test


The Burn-In of Heating test is used when a new heating block
is installed.
1. From the Contents tab select the Burn-In of Heating Test.

2. Select the Test Configuration tab and select the heating


block to burn in.

3. Select Start to initiate the procedure.


4. The procedure takes approximately 2.5 hours.

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Section 11 Incubation Device

OPTIONAL Test: Heating Validation with TEMPO110


This test is used to verify heater block performance using the
TEMPO110 measuring device.
1. From the Main menu, select <Options><Incubation
Device>. The Incubation Device panel displays.

2. From the Contents tab select the Heating Validation


with TEMPO110 (optional) checkbox (聺) under Test:.
3. Switch to the Test Configuration tab.
NOTE: The temperature device default setting is
50°C.

4. If 50°C is not the desired temperature, double click on the


50°C device and change to 75°C.
5. Enter a two to four character name in the 'Operator Alias'
field. Select Apply to save the settings.
6. Select Start to initiate the protocol.
7. The operator is prompted to connect the TEMPO110
temperature measuring device.
8. The test takes about 45 minutes to finish. When the test is
completed, the operator is notified that the test passed or
failed.

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Genesis Setup and Service Software Section 11

9. The outcome of the test can be viewed in the Results page.


Criteria for passing:
• Limit 50°C = ± 1.4
• Limit 75°C = ± 2.3
• Timeout = 45 minutes
10. Results of the test can be printed by selecting the Print
checkbox (聺).
11. Results of the test can be saved by selecting the Save As
key.

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Section 11 SMIO for NAT

SMIO for NAT


WARNING: Potential Biohazard. Indicates an area
where the FSE or operator could be exposed to
potentially infectious human-sourced material.
Contamination hazard due to parts of instrument that
are not completely decontaminated. Wear appropriate
personal protective equipment such as gloves, lab coat,
and protective eye wear.
CAUTION: Chemical Hazard. The FSE or operator
could be exposed to hazardous chemicals when
handling Abbott Molecular sample preparation
reagents. Exposure to hazardous chemicals is
minimized by observing instructions provided in
product-specific labels and product-specific Material
Safety Data Sheets (MSDS). Wear appropriate personal
protective equipment, such as gloves, lab coat, and
protective eye wear.

Calibration of the DiTi Waste Sensor


1. Go to the <Options><SMIO for NAT> menu.

2. Select DiTi Waste Sensor Calibration under Setup.

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Genesis Setup and Service Software Section 11

3. Go to the Tools & Configuration tab.


4. Verify the Device Type is NAT. If not, select NAT from the
pull down menu. Select Diti Waste Full. Clear the other
checkboxes.

5. Select Start. The prompt Place a reflecting plate at about


120 (mm) below the ultrasonic sensor displays. Instead
of placing the plate at 120 mm, place the plate at 300 mm
below the sensor.

6. Select Next. The prompt Turn both potentiometers


counterclockwise to their stop positions displays.
Instead of placing the plate at 120 mm, place the plate at
340 mm below the sensor.

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Section 11 SMIO for NAT

7. Select Next. The prompt Adjust the distance


potentiometer (top potentiometer) to the point where
the integrated LED just lights up displays.

8. Select Next. The prompt Setup: Done displays.

9. Select OK.
NOTE: If the calibration fails, check the
connection to the SMIO board and repeat the test.
NOTE: If the calibration fails, replace the sensor.

Adjustment of System Liquid Level Sensors


Potentiometers
NOTE: System liquid level sensing is accomplished by
changes in capacitance. Water droplets on the inside
and outside of the liquid container can adversely affect
potentiometer adjustment and sensor calibration. The
waste liquid sensor is an optical sensor.
1. Remove the two screws securing the potentiometer to the
bracket.
2. Pour 1 L of water into the container and allow the water to
settle.
3. Move the sensor to the left to access the potentiometer
adjustment screw located on the rear face of the sensor.

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Genesis Setup and Service Software Section 11

4. Keep the sensor at the same level of the mounting


brackets.
5. Hold the sensor in place with one hand or use the
container to hold the sensor in place against the bracket.
6. With the container in place, adjust the potentiometer so
the sensor light is off.
7. Adjust the potentiometer in the opposite direction until
the light comes on.
8. Add 1 L of liquid to the container, for a total of 2 L in the
container.
9. Verify the sensor light is off.
10. Attach the sensor to the bracket and perform the sensor
verification procedure detailed below.

Waste Liquid Sensor Potentiometer Adjustment


1. Disconnect the waste container hoses and remove the cap.
2. Pour 8.5 L of water into the container and allow the water
to settle.
3. Switch the instrument on.
4. Adjust the potentiometer, located on the end of the
sensor, counter clockwise until the sensor LED goes out.
5. Turn the potentiometer clockwise slowly until sensor LED
turns on.
6. Turn the potentiometer clockwise another 180 degrees
(half turn).
7. Add 1.5 L of liquid to the container, for a total of 10.0 L in
the container.
8. Verify the sensor LED is on.
9. Pour out the liquid. Dry the outside surface of the
container, but leave the inside wet.
10. Place the container back in position and check to ensure
the sensor light is off.
11. If the sensor is on perform a minor adjustment of the
potentiometer until the sensor is off.
12. Repeat steps 1 through 11 to verify accuracy of the
potentiometer adjustment.
13. Perform the Sensor Verification Procedure detailed below.

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Section 11 SMIO for NAT

14. Replace the sensor only after two (2) consecutive failed
adjustment attempts.

Rapid Sensor Test


1. Go to the <Options><SMIO for NAT> menu.

2. Go to the Tools & Configuration tab and select the


box(es) of the sensor(s) to check.
NOTE: If more than one checkbox is selected, tests
are performed sequentially. If a test fails, continue
with Accurate Sensor Verification.

System Bottle Empty


1. Remove the system bottle. The panel displays yes and the
box is darkened.

2. Place your hand in front of the sensor. The panel changes


to no.
NOTE: If the change occurs, the sensor is
functioning.

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Genesis Setup and Service Software Section 11

Wash Bottle Full


1. Remove the wash bottle and place your hand in front of
the sensor.
2. The panel changes from no to yes' and the box is
darkened.

NOTE: If the change occurs, the sensor is


functioning.

DiTi Waste Full


Hold your hand just below the sensor. The panel changes from
no to yes and the box is darkened.
NOTE: If the change occurs, the sensor is functioning.

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Section 11 SMIO for NAT

Accurate Sensor Verification

System Bottle
1. Go to the <Options><SMIO for NAT> menu.
2. Select the Sensors Test checkbox (聺).

3. Go to the Tools & Configuration tab and select the


System Bottle Empty checkbox (聺).
4. Select Start. The prompt Fill system bottle with 1.0 [liter]
of liquid displays.

5. Select Next. The prompt Add 2.0 [liter] of liquid displays.

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Genesis Setup and Service Software Section 11

6. Select Next. If the test was successful, the prompt Sensor


Test: Passed displays.

7. If the test fails, adjust the system liquid sensor


potentiometer as described in the previous section.

Liquid Waste Bottle


1. Go to the <Options><SMIO for NAT> menu.
2. Select the Sensors Test checkbox (聺).
3. Go to the Tools & Configuration tab and select the Wash
Bottle Full checkbox (聺).
4. Select Start. The prompt Fill waste bottle with 8.5 [liter]
of liquid displays.

5. Select Next.
6. The prompt Add 2.0 [liter] of liquid displays.

7. Select Next.

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Section 11 SMIO for NAT

8. If the test was successful, the prompt Sensor Test: Passed


displays.

DiTi Waste Sensor Verification


1. Go to <Options><SMIO for NAT>.
2. Select the Sensors Test box.
3. Go to the Tools & Configuration tab and check the DiTi
Waste Full box.
4. Select Start. The prompt Place a reflecting plate at
180 [mm] below the sensor displays. Instead of placing
the plate at 180 mm, place the plate at 360 mm below the
sensor.

5. Select Next. The prompt Place the plate at 120 [mm]


below the sensor displays. Instead of placing the plate at
120 mm, place the plate at 300 mm below the sensor.

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Genesis Setup and Service Software Section 11

6. Select Next. If the test was successful, the prompt Sensor


Test: Passed displays.

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Section 12: Spare Parts and Accessories

SECTION 12:
SPARE PARTS AND
ACCESSORIES

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Spare Parts and Accessories Section 12

NOTES

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200942-103—September 2007
Section 12 Table of Contents

Table of Contents
TOC
Purpose of This Section . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-4
Spare Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
System Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-5
Power Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-6
X-Drive Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-6
Liquid Handling Arm (LiHa) . . . . . . . . . . . . . . . . . . . . . 12-7
RoMa Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-9
PosID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-9
Liquid System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-11
Fast Wash (FaWa) Pump . . . . . . . . . . . . . . . . . . . . . . . 12-11
Dilutors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-11
Access Option and Waste Monitoring . . . . . . . . . . . . 12-12
1 mL Heat-Mag Subsystem (1 mL Subsystem) Components
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-12
Computer, Monitor, and Printer . . . . . . . . . . . . . . . . . 12-13
Cabinet and Instrument Covers . . . . . . . . . . . . . . . . . 12-14
Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-15
Preventive Maintenance Kit . . . . . . . . . . . . . . . . . . . . 12-15
Accessories and Commodities . . . . . . . . . . . . . . . . . . . 12-16
Output Deck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-16
Upgrade Kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Abbottlink and MxN . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17
Hood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-17

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Spare Parts and Accessories Section 12

Purpose of This Section

This section lists and identifies spare parts and accessories


needed for maintenance and repair of the m2000sp G-series.
Ordering information is also included.
NOTE: The Field Replaceable Unit Database found on
the Global Service and Support website maintains the
most current part numbers and descriptions.

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Section 12 Spare Parts List

Spare Parts List


Green shading indicates parts that can be used on both the
E-series and the G-series models.

Worktable
Part Description Number
WORKTABLE, ASPS Stainless steel; worktable cutout at 619730
grid 18
PIN SET GENESIS Includes 25 guide pins, 20 stop pins, 04J97-36
5 reference guide pins, and 5 carrier
lock reference pins
PIN LOCK FROG 100 PCE Set of 100 619017
GENESIS/MINI PREP
COVER SCREW WHI. 20 PCE, Screw covers for the worktable; set of 619103
WORKTABLE GENESIS 20
STRIP 200CM SEAL WASTE CHANNEL Rubber sealing strip for the worktable; 619003
WORKTABLE 200 cm
WASH STATION Includes tube connectors 04J72-28
Metal Guide Pins Set 50-148040
PIN LOCK FROG SET 16 PCE 10619007
PIN STOP 100 PCE. GENESIS 10619030
WORKTABLE
PIN GUIDE SET 25 PCE. WORKTABLE 10619439

System Electronics
Part Description Number
PCB OPTIBO FREEDOM 619856
FUSE 6.3 AT SET 10 PCE 6.3 AT, set of 10 619821
FUSE 8A 10 PCE 8 AT, set of 10 619823
PCB CU GENESIS 619828 or
10619301
CABLE PC-OPTIBO 619232

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Spare Parts and Accessories Section 12

Power Module
Part Description Number
POWER SUPPLY 2 960W RWS/FREEDOM 100-240 VAC, 50-60 HZ, 960 W power 619844
150/200 supply, complete with bracket, lamp
and switch
POWER CABLES, SWISS 04J97-16
POWER CABLES, EUROPE 04J97-18
POWER CABLES, USA 04J97-20
POWER CABLES, UK 04J97-22

X-Drive Assembly
Part Description Number
X-DRIVE COMPLETE GENESIS 619854
FREEDOM
X-BELT 16/250 GENESIS FREEDOM 619855

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Section 12 Spare Parts List

Liquid Handling Arm (LiHa)


Part Description Number
ARM LIHA 8CH. REPL. Genesis Freedom Includes 8 Z-racks and 8 Tip Adapter 619860
Y-BELT LIHA ARM 2 PIECE GENESIS Y-belt for LiHa Freedom; set of 2 619215
PCB DC SERVO 1 For LiHa; RoMa; Z,Y; PosID; Y/B 619303
PCB DC SERVO POWER 1 GENESIS For LiHa and RoMa 619829 or
10626201
PCB LIHA ARM GENESIS FREEDOM 619864
PCB ILID FIRST LIHA FDM EVO-2 30014383
CABLE ILID PCB-BACKPLANE GENESIS ILID board to Backplane 619861
FREEDOM
CABLE ILID SET 4 PCE GENESIS Set of 4 619863
FREEDOM
CABLE FLEX LIHA/ROMA 13 PIN Flex cable for LiHa/RoMa; 13 pins 619866
FREEDOM 150
MOTOR COMPLETE Y-AXIS LiHa Servo motor for LiHa Y-axis 30019824
GENESIS
TUBING SUPPORT LIHA SET 4 P 30014387
GENESIS FDM
MOTOR DC LIHA ARM Z-AXIS + POSID Servo motor for LiHa Z-axis and 619323
Y-AXIS PosID 2 Y-axis
DITI Spare Set Includes 8 steel adapters, 8 plastic 04J97-38
(Option DiTi Cone ASPS) tubes, 8 needles, 8 cones, 8 O-rings,
and 8 separator rings. For V.1 DiTi
cones
OPTION DITI CONE ASPS V2 Includes 8 steel adapters, 8 plastic 30027324
tubes, 8 needles, 8 cones, and
10 O-rings. For V.2 DiTi cones
ADAPTER TIP GENESIS FREEDOM 619893
CABLE ROUND LIHA GENESIS 619876
FREEDOM 100/150
OPTION LOWER EJECTION DITI Lower DiTi eject option 612550
MAGNET LOWER EJECTION DITI Solenoid for the lower DiTi eject 619520
OPTION option
COVER SLIDE DITI WASTE 10613030
KIT LOWER DITI UPDATE 10619361
OPTION DITI CONE 8 PCE AND 30026919
WRENCH V2

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Spare Parts and Accessories Section 12

Part Description Number


O-RING DITI 10 PC V2 Includes 10 O-rings. For V.2 DiTi 30026922
cones.
DITI SLIDE For wash station 04J72-29
RING SEAL SET 8P. + O-RING 8P. DITI Includes 8 O-rings and 8 separator 619508
OPTION rings. For V.1 DiTi cones.
NO DITI WRENCH LABEL 50-148319
GUIDE TUBING GENESIS FREEDOM 619853
m1000/m2000sp Support Tubing 30014404
Upgrade
SHAFT DRIVE STAR-SHAPED LiHa 4 PCE. 30026358
PINION Z-GEAR STAR-SHAPED SHAFT 4 30026360
PCE.

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Section 12 Spare Parts List

RoMa Standard
Part Description Number
Roma Grippers RoMa fingers, screws, and 3M tape 50-148125
PCB GRIPPER ROMA Gripper board 619300
MOTOR ROTARY DC SERVO ROMA Rotator motor 619345
OPTION
CABLE FLEX 10 PIN LONG ROMA For gripper interface; 1.61 m, 10 pins 619877
GENESIS FDM
PCB DC SERVO 1 For LiHa; RoMa Z, Y; and PosID Y/B 619303
PCB ROMA GENESIS FREEDOM 619868
Y-BELT ROMA OPTION Y-belt, RoMa 619513
MOTOR COMPLETE Y-AXIS ROMA Motor for LiHa Z-axis and PosID 2 30019825
Y-axis
Z-MOTOR ROMA OPTION Z-motor 619346
BRAKE SOLENOID Z-AXIS COMPLETE Active brake 30027047
ROMA-3
SET ROMA Z BRAKE M2000EVO Brake shoe and spring only 30031034
CABLE FLEX LIHA/ROMA 13 PIN Flex cable for LiHa/RoMa; 13 pins 619866
FREEDOM 150
PCBA DEVICE CU BOARD 1 619050
ARM ROMA SPARE GENESIS FREEDOM RoMa arm, excluding gripper finger 10619888
and flex cable
PCB BACKPLANE ROMA 2 OPTION 619343
PCB DC SERVO POWER 1 GENESIS 619829 or
10626201
Cable Flex Y-axis Back-back Roma FDM. Flex cable Y-axis backplane to RoMa 619339
Freedom backplane
GRIPPER MP OBJECTS OPTION ROMA 612601
ARM

PosID
Part Description Number
Option PosID2 Genesis Entire assembly with pan and X-drive 10612412
X-BELT 5M POSID2 X-belt for PosID 2; 5 m 619611
CABLE FLEX POSID2 GENESIS 100/150 Flex cable 619602

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Spare Parts and Accessories Section 12

Part Description Number


MOTOR COMPLETE X-AXIS POSID2 Servo Motor for LiHa Y-axis and PosID 30019821
2 X-axis
PCBA CU POSID2 Control unit 619600
PCB Y/B POSID2 619605
PCB DC SERVO 1 For LiHa; RoMa Z,Y; PosID Y/B 619303
PCB DSP POSID2 Decoder board 619607
MOTOR DC SCANNER DRIVE POSID2 Scanner motor for PosID 2 B-axis 10619601
SCANNER LASER POSID2 Laser scanner without DSP board 619610
CABLE SCANNER POSID2 Scanner cable 619609
Y-BELT POSID2 Y-belt 619612
GRIPPER POSID2 PosID rack gripper 619622
MOTORS COMPLETE Y-AXIS POSID2 Servo motor for LiHa Z-axis and 30019823
PosID 2 Y-axis
SENSOR TUBE POSID2 Includes casing, sensor, and cable 619606
POSID MODULE W/O POSID Base and X-drive not included 619608
BASE/X-DRIVE

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Section 12 Spare Parts List

Liquid System
Part Description Number
Y-Distributor 2 Out. MPO Set 5P Genesis 10 mm Y-distributor; set of 5 619410
Tube Aspirating PVC 6.9 MM 5000 MM 619401
Genesis
BLOCK CONNECTOR LIQUID POM 1:4 619406
1/0 M6
TUBE INTERCONNECTING M6 650 MM Tubing to dilutor 619402
GENESIS
Tube Pipetting FEP M6 2.5 1.5 mm 2800 Pipetting tubing 619403
mm
Clamp 9.1 + 9.6 mm 2 SET 10 Pce. 9.1 and 9.6 mm diameter tubing 619414
Genesis clamp sets; 10 each

Fast Wash (FaWa) Pump


Part Description Number
FWO 4/8 TIP Complete 612704
PUMP MEMBRANE PP/FFPM Spare fast wash pump 619509
REPLACEMENT FWO
PCB MPO FAST WASH + LICOS For FaWa pump 619312
CABLE CONNECT FWO/MPO RIGHT OPTIBO to MPO board cable 619415
RSP 100/150
Y DISTRIBUTOR 2 OUT.MPO SET 5P. 619410
GENESIS
CONTACT STRIP MPO 12CM Copper MPO grounding strip 619898
Tube FaWa Silicon 5.8mm 5000mm 619405
Genesis
VALVE PRESSURE-RELIEF PP 15.5N/CM2 10619523
KIT FAWA MAINT. SINGLE HEAD PUMP 10619434
GENESIS
TUBE WASTE PVC 9*12MM 2500MM 10619404
GENESIS

Dilutors
Part Description Number
DILUTOR XP SMART Includes M6 fittings 10649015

m2000sp G-series Service Manual 12-11


200942-103—September 2007
Spare Parts and Accessories Section 12

Part Description Number


VALVE 3-WAY 120° M6 DILUTOR XP 10649016
SMART
PCB VCC 8 DILUTOR XP3000 PLUS Backplane for all 8 dilutors 619259
SYRINGE 1.0 mL 04J97-44

Access Option and Waste Monitoring


Part Description Number
LOCK DOOR L+R FRONT Right and left front shield 619847
SH.RSP/RWS/NPS/FDM
PCB SMIO GENESIS SMIO-SAFY Board for door locks 619850
RSP/RWS/NPS/FREEDOM
CABLE COMMUN. SMIO PCB GENESIS CAN-BUS Cable from OPTIBO Board 619894
FREEDOM to SMIO/SAFY Board
PCB SMIO GENESIS FE500 SMIO for sensors 10626207
CABLE, CAN 0.6M CAN-BUS Cable from SMIO/SAFY 619740
Board to SMIO Board
SENSOR CAPACITIVE 3M ASPS Sensor for System Liquid Container 619741
SENSOR ULTRASONIC 2 MASPS Sensor for Solid Waste Container 619742
SENSOR OPTICAL 3M ASPS Sensor for Liquid Waste Container 619777

1 mL Heat-Mag Subsystem (1 mL Subsystem) Components


Part Description Number
MAG.HEAT SUBSYSTEM Complete 1 mL Subsystem, without 619743
OPTIBO Cables
CABLE SUBSYSTEM ASPS Cable connecting the OPTIBO to the 619749
CC Board; quantity: 1
PCBA COMPLETE TE-MAGS. Control Center Board 760712
BLOCK MAGNET ASPS Magnetic Block and Cover 619745
BLOCK HEAT ASPS 619744
COVER RT BLOCK ASPS RT Block 619746
CABLE HEAT BLOCK ASPS Cable Connection for Heater Block 619750
SUBSYSTEM CARRIER 04J97-50
SUBSYSTEM CARRIER COV 04J97-51
COVER HEATING BLOCK ASPS 619757

12-12 m2000sp G-series Service Manual


200942-103—September 2007
Section 12 Spare Parts List

Computer, Monitor, and Printer


Part Description Number
m2000 SCC RAD945, CPU 01L67-02
FLAT PANEL DISPLAY 09K99-01
Keyboard – English, Tec 04J72-94
m1000 m2000 System Keyboard, SCC 04J72-96
(Spanish)
m1000 m2000 System Keyboard, SCC 04J72-98
(French)
m1000 m2000 System Keyboard, SCC 04J97-02
(Italian)
m1000 m2000 System Keyboard, SCC 04J97-04
(German)
m2000 Keyboard SCC (with optical KEYBOARD, SCC 01N04-01
mouse)
m2000 Keyboard (with optical mouse) KEYBOARD, SCC SPANISH 01N03-01
(SPANISH)
m2000 Keyboard (with optical mouse) KEYBOARD, SCC FRENCH 01N00-01
(FRENCH)
m2000 Keyboard (with optical mouse) KEYBOARD, SCC ITALIAN 01N02-01
(ITALIAN)
m2000 Keyboard (with optical mouse) KEYBOARD, SCC GERMAN 01N01-01
(GERMAN)
m1000 m2000 System SCC Component 06L77-01
Stand
m1000 m2000 Printer 220V 06L79-02
m1000 m2000 Printer 110V 06L79-01
M2000 Mouse 03L33-01
PARALLEL PRINTER CABLE 50-148129
NETWORK CROSSOVER CABLE, 15M 50-148060
NETWORK CROSSOVER CABLE, 100M 50-148065
5-port Ethernet Switch 50-148070
PC Anywhere and XPe License Labels 50-148200
EDGEPORT FOR CHILLER 50-148128

m2000sp G-series Service Manual 12-13


200942-103—September 2007
Spare Parts and Accessories Section 12

Cabinet and Instrument Covers


Part Description Number
VS Cabinet Assembly 04J72-06
Door Left Cabinet Asps Plexiglas 619732
Door Right Cabinet Asps Plexiglas 619733
GUIDE RAIL DRAWER WASTE ONE PAIR 619734
CHAIN ENERGY WASTE DRAW 619735
Magnet Waste Drawer 10 PCE Set of 10 619736
HINGE SET 10 PCE. ACRYLIC GLASS Front cover hinges; set of 10 619463
COVER
GUIDE TUBING GENESIS FREEDOM 619853
BLIND WASTE FRONT GENESIS RSP 150 Waste blind 619437
System Liquid Assembly Includes tubing and connections 04J72-12
Liquid Waste Assembly Includes tubing and connections 04J72-10
(labels not included: order #
04J97-62)
Container, 10L Liquid For system liquid or liquid waste 04J72-08
(labels not included: order #
04J97-62)
Container 50 L Sld Wast Includes cover 04J72-14
HANDLES AND BRACKET FRONT 619049
COVER ASPS
STRIP 200CM SEAL WASTE CHANNEL 619003
WORKTABLE
PANE LEFT/RIGHT ACRYLIC GLASS 30016640
M2000™
PANE TOP ACRYLIC GLASS M2000 30016642
PANE FRONT ACRYLIC GLASS M2000 30016644
SPRING GAS FRONT PANEL FREEDOM 10649031
EVO
SCREW SHELF POSITION 18 PCE 30016645

12-14 m2000sp G-series Service Manual


200942-103—September 2007
Section 12 Spare Parts List

Tools
Part Description Number
ROMA GRIPPERS TAPE 3M Tape for Reference Plate 50-148126
TIP REFERENCE STEEL SHORT LiHa Reference Tip (1 pc) (new style) 30026915
TUBE GREASE 2 PCE. SYS GENESIS Lubrication Grease 10626104
FE500
TOOL CALIBRATING ROMA RoMa Calibration Tool 612622
TOOL ADJUSTMENT ROMA RoMa Teach Plate 613101
RACK SERVICE POSID 613100
HANDLE TRANSPORT SET GENESIS Transport handle set 612003
SCREW SET ASPS Includes 5 each of the following: 619751
M 2x6 mm, M 2x8 mm, M 2.5x6 mm,
M 3x5 mm, M 3x8 mm, M 3x10 mm,
M 4x10 mm, M 4x12 mm,
M 3x12 mm, M 4x8 mm, and
M 4x20 mm
LIHA WRENCH HOLDER 8-69545-01
LIHA Y-SPREAD AID 8-69546-01
REAGENT NEST CALIBRATION TOOL 50-148020
LOCK TRANSPORTATION FREEDOM 30024075
EVO 2 PCE.
PACKAGING BOX GEN.FRDM & FREE- 30024077
DOM EVO

Preventive Maintenance Kit


Part Description Number
KIT PREVENTIV MAINTENANCE ASPS Kit includes all replacement parts 619912
required to perform routine 6 month
and annual maintenance, with the
exception of DiTi cones. Includes
old-style Dilutor valves (04J97-42)
VALVE 3-WAY 120° M6 DILUTOR XP 10649016
SMART
DITI Spare Set 04J97-38
OPTION DITI CONE ASPSV2 30027324

m2000sp G-series Service Manual 12-15


200942-103—September 2007
Spare Parts and Accessories Section 12

Accessories and Commodities


Part Description Number
Carrier Additiv Trough 100 mL trough for m1000 04J72-24
Assy. Carrier 200 ML Reagent Vessel D-57226-103
Carrier, Mp Roma Wide 04J72-26
Wash Station 04J72-28
Rack, Robotic DiTi 200 04J72-30
Rack, Robotic DiTi 1000 04J72-32
Rack, DiTi Reuse 1000 04J72-34
Striprack 13 mm Tubes 04J72-82
Striprack 16 mm Tubes 04J72-86
Deepwell Plate, DiTi Reuse 04J97-28
ASPS 1000 µL (1 mL) Pipette Tip 04J71-10
200 µL Pipette Tips 04J71-17
Labels, Barcode 04J97-52
Labels, Container 04J97-62
m2000sp Sample Rack Label Kit 30019855
TSB label 50-602396
Promega RNA Labels 50-148301
m2000 System 13 mm PoSt set Set includes 6 standard + 6 custom 01N93-01
m2000 System 16 mm PoSt set Set includes 6 standard + 6 custom 01N94-01

Output Deck
Part Description Number
Peltier Output Deck Assy. Chiller unit D-57217-104
m2000sp Reagent Nest 50-148050
PCR PLATE FOAM INSULATION, 01N98-01
REPLACEMENT
CABLE CHILLER PSU 12V 10619928
PCR Bumpstop 50-148131

12-16 m2000sp G-series Service Manual


200942-103—September 2007
Section 12 Spare Parts List

Upgrade Kits
Part Description Number
m2000 m1000 Upgrade Kit 09K13-01
m2000sp Worktable Kit 6L60-03
Wheels 8-200918-01
m2000 System Hardware Kit 50-148320

Software
Part Description Number
m2000sp System Software, SCC Series D, 50-148133
Release 3.0
m2000sp System Software, SCC RAD945, 50-148132
Release 3.0
P24 BIOS Upgrade, SCC Series D 50-148140

Abbottlink and MxN


Part Description Number
m2000 Abbottlink Setup 02N37-01
m2000 System Network 02N83-01
Abbottlink PC 50-148332
Hardware Firewall 50-148333
RJ-45 to RJ-45 Cat53 LAN cable (50 ft) 50-148334
RJ-45 to RJ-45 Cat53 LAN cable (100 ft) 50-148335
RJ-45 to RJ-45 Cat53 LAN cable (5 ft) 50-148336
RJ-45 to RJ-45 Cat53 LAN cable (14 ft) 50-148337
RJ-45 to RJ-45 Cat53 LAN cable (25 ft) 50-148338

Hood
Part Description Number
m2000sp Hood Assembly G-Series 02N29-01
Hood Patlite Signal Tower with 50-148340
connector
Hood assembly, gullwing door 50-148341
Hood assembly, C-arm support with 50-148342
window

m2000sp G-series Service Manual 12-17


200942-103—September 2007
Spare Parts and Accessories Section 12

Part Description Number


Hood molecular control board AMC_1 50-148330
Hood power cable assembly 50-148344
Hood serial cable for patlite signal tower 50-148345
Hood assembly window front 50-148346
Hood light switch jumper cable 50-148347
Hood lamp fluorescent 50-148348
EMC Upgrade for hood G-Series 50-148349

12-18 m2000sp G-series Service Manual


200942-103—September 2007
Appendix Passwords

APPENDIX:
PASSWORDS

m2000sp G-series Service Manual Appendix-1


200942-103—September 2007
Appendix

Appendix

NOTES

Appendix-2 m2000sp G-series Service Manual


200942-103—September 2007
m2000sp User Account Passwords

m2000sp USER ACCOUNT PASSWORDS


Day FSE CSC
1 8151 11
2 9321 12
3 9101 13
4 7291 14
5 7112 15
6 3331 16
7 5361 17
8 7312 18
9 1421 19
10 8291 20
11 8122 21
12 4261 22
13 7271 23
14 3131 24
15 7212 25
16 9381 26
17 5271 27
18 7251 28
19 0222 29
20 5301 30
21 0381 31
22 2341 32
23 9322 33
24 2181 34
25 4171 35
26 9191 36
27 9251 37
28 3301 38
29 3122 39
30 2331 40
31 3181 41

CONFIDENTIAL! NOT TO BE GIVEN TO OR LEFT WITH CUSTOMERS!

m2000sp G-series Service Manual Appendix-3


200942-103—September 2007
Appendix

NOTES

Appendix-4 m2000sp G-series Service Manual


200942-103—September 2007

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