Name: Kim Bryan B.

Ngoho Date: June 14, 2021

Laboratory 1: Light Microscope (Basic Microscopy)

Learning Objectives
Each student should be able to:
1. Identify all the parts of a compound microscope
2. Know how to correctly use the microscope— especially the oil immersion
lens

Parts of a Compound Microscope

1. Your microscope may have all or most of the features described above. By
studying, label the compound microscope in figure LR1.1 on the next page.
Locate the indicated parts of your microscope and answer the following
questions.
a. What is the magnification stamped on the housing of the oculars of a
microscope?
= 10

b. What are the magnifications of each of the objectives of a microscope?

= 4, 10, 40, 400

c. Calculate the total magnification for each ocular/objective combination of a

microscope.
Ocular × Objective = Total Magnification
___________________
10 _______________
4 __________________________________
40
___________________
10 10
_______________ __________________________________
100
___________________
10 _______________ __________________________________
40 400
___________________
1010 _______________
400 __________________________________
4000
d. List the magnification and numerical aperture for each objective of a
microscope.
Magnification of Objective Numerical Aperture (NA)
____________________________________
4 .10
____________________________________
____________________________________
10 .25
____________________________________
____________________________________
40 ____________________________________
.65
400 1.25
____________________________________ ____________________________________

e. With some compound microscopes, loosening a lock screw allows you to

rotate the body tube 180°. What is the advantage of being able to rotate the
body tube?
= Rotating the body tube allows you to share your findings with others
without having to move the entire microscope.

f. Where is the diaphragm of a microscope located?

= Top is the light source between light source and stage.
 g. How can you regulate the diaphragm?
= Rotating dial and Rotate silver knob.

h. Can the light intensity of a microscope be regulated? Explain.

= Light adjustment knob.

Ocular Lens

Body
Arm

Nose Piece

Objective Lens

Mechanical Stage
Coarse adjustment
Condenser
Fine adjustment
Diaphragm

Base with light source Stage Adjustment Knobs

Field Diaphragm lever

Light Intensity Control
Name: ___________________________________ Date: Date: June 14, 2021

Laboratory 2: Microscopic Measurement of Microorganisms

Microorganism Length Width Magnification

Bacterium name ___________________ __________________ __________________
Fungus name ___________________ __________________ __________________
Alga name ___________________ __________________ __________________
Protozoan name____________________ __________________ __________________

1. Draw and label, as completely as possible, the microorganisms that you

research.

Genus and species: ______________ Genus and species: _____________

Magnification: ____________________ Magnification: ____________________

Genus and species: ______________ Genus and species: _____________

Magnification: ____________________ Magnification: ____________________

Review Questions

1. What are three bacterial shapes you observed?

______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________

2. In microbiology, what is the most commonly used objective? Explain your

answer.
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________

3. In microbiology, what is the most commonly used ocular? Explain your

answer.
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________

4. In a prepared slides, which organism was the largest?

______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________

5. When identifying microorganisms, why should a wet-mount be used

when making measurements?
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
Name: ___________________________________ Date: Date: June 14, 2021

Laboratory 3: Major Challenges Facing Microbiology Today

1. Describe three major challenges facing microbiology today.

Name: ___________________________________ Date: Date: June 14, 2021

Laboratory 4: Microbiologists

Differentiate the contributions to microbiology made by Antony van

Leeuwenhoek, Redi, Needham, Spallanzani, Pasteur, Jenner, Koch,
Semmelweis and Snow, Fleming, Florey and Chain, Domagk, and Waksman.