Nucleic Acids, Part B: Methods in Enzymology

Methods in Enzymology
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Nucleic Acids, Part B
Lawrence Grossman, Kivie Moldave

Volume 12, Part B,
Pages 3-972 (1968)
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Contributors to volume XII, part B
Pages v-ix
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Editorial No access
Preface
Lawrence Grossman, Kivie Moldave
Page xi
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Previous volumes
Page xx
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Volumes in preparation
Page xxi
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Erratum Full text access

Errata for volume XII, Part A
Page xxii
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[96] Isolation and characterization of chromosomal nucleoproteins
James Bonner, G. Roger Chalkley, Michael Dahmus, Douglas Fambrough, ... Jack Widholm
Pages 3-65
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[97] Isolation and purification of nuclear proteins
Harris Busch
Pages 65-84

[98] Isolation of nucleic acids with phenolic solvents
K.S. Kirby
Pages 87-99

[99] The isolation of nucleic acids from bacterial spores
I. Takahashi
Pages 99-100

[100] Isolation and purification of plant nucleic acids from whole tissues and from
isolated nuclei
Herbert Stern
Pages 100-112

[101] Isolation of deoxyribonucleate from invertebrates
Robert P. Klett, Michael Smith
Pages 112-115

[102] Isolation of mammalian nuclear nucleic acids
Tung Yue Wang
Pages 115-120

[103a] The use of guanidinium chloride in the isolation of nucleic acids
R.A. Cox
Pages 120-129

[103b] The use of sodium and lithium dodecyl sulfate in nucleic acid isolation
Hans Noll, Erhard Stutz
Pages 129-155

[104] Recovery and purification of nucleic acids by means of
cetyltrimethylammonium bromide
A.R. Bellamy, R.K. Ralph
Pages 156-160
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Previously published articles from Methods in Enzymology related to Section VII
Page 162
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[105] Determination of DNA concentration with diphenylamine
K. Burton
Pages 163-166

[106a] Rapid miroscale assay for sRNA
Joseph D. Cherayil, Arnold Hampel, Robert M. Bock
Pages 166-169

[106b] Filter paper disk techniques for assaying radioactive macromolecules
F.J. Bollum
Pages 169-173

[106c] Membrane filter technique for the assay of “charged” sRNA
Jesse F. Scott
Pages 173-177

[107] The determination of nucleic acid base composition by chemical reactivity
Shih Yi Wang
Pages 178-184

[108] Use of CsCl density gradient analysis for determining the guanine plus
cytosine content of DNA
M. Mandel, C.L. Schildkraut, J. Marmur
Pages 184-195

[109] Use of ultraviolet absorbance-temperature profile for determining the
guanine plus cytosine content of DNA
M. Mandel, J. Marmur
Pages 195-206

[110] Polynucleotide 5′-hydroxyl kinase
J. Hurwitz, A. Novogrodsky
Pages 207-212

[111] Alkaline phosphatase of Escherichia coli
A. Torriani
Pages 212-218

[112] Chain length determination by end to total phosphorus
Edna Seaman
Pages 218-220

[113] Nucleoside diphosphate end-group analysis using alkaline hydrolysis or
nuclease digestion
G.M. Tener
Pages 220-224

[114] Nucleoside end determinations in nucleic acid analysis
H. Fraenkel-Conrat
Pages 224-227

[115] End-group modifications of sRNA with snake venom phosphodiesterase
Geoffrey L. Zubay
Pages 227-229

[116a] Periodate oxidation of ribonucleic acids and their derivatives
Gerhard Schmidt
Pages 230-235

[116b] Borohydride reduction of periodate-oxidized chain ends
Stephen H. Leppla, Barbara Bjoraker, Robert M. Bock
Pages 236-240

[117] The use of isoniazid for the determination of terminal sequences in
periodate-oxidized RNA
J.A. Hunt
Pages 240-243

[118] Stepwise degradation of RNA: Periodate followed by aniline cleavage
H. Fraenkel-Conrat, A. Steinschneider
Pages 243-246

[119] Ultraviolet spectral analysis of nucleic acids
Gary Felsenfeld
Pages 247-253

[120] Solvent perturbation spectra of nucleic acids
R.M. Bock, J.L. Hoffman
Pages 253-256

[121] Use of infrared spectroscopy for the measurement of nucleoside binding to
polynucleotides
H. Todd Miles
Pages 256-267

[122] Optical rotatory dispersion as a means of determining nucleic acid
conformation
Alice J. Adler, Gerald D. Fasman
Pages 268-302

[123] Ultraviolet circular dichroism in nucleic acid structural analysis
Wilfred F.H.M. Mommaerts
Pages 302-329

[124] Use of cesium sulfate for equilibrium density gradient centrifugation
Waclaw Szybalski
Pages 330-360

[125] Monolayer techniques in electron microscopy of nucleic acid molecules
A.K. Kleinschmidt
Pages 361-377

[126] Electron microscopic determination of the length of single polynucleotide
chains
Michael Beer
Pages 377-379

[127] Measurement of nucleic acid hydrogen exchange
S. Walter Englander
Pages 379-386

[128] Molecular weight and conformation of DNA
Joseph Eigner
Pages 386-429

[129] Molecular weight and conformation of RNA
Helga Boedtker
Pages 429-458

[130] The selective photoreduction of uridine in ribonucleic acid
P. Cerutti
Pages 461-467

[131] The reaction of formaldehyde with denatured DNA
Lawrence Grossman
Pages 467-480

[132] sRNA methylases from Escherichia coli
Jerard Hurwitz, Marvin Gold
Pages 480-491

[133a] DNA methylase from Escherichia coli
Marvin Gold, Jerard Hurwitz
Pages 491-496

[133b] Isolation and properties of enzymes involved in glycosylation of
bacteriophage DNA
John Josse
Pages 496-510
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Previously published articles from Methods in Enzymology related to Section IX
Page 512
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[134] Polynucleotide phosphorylase from Escherichia coli
Yosef Kimhi, Uriel Z. Littauer
Pages 513-519

[135] Preparation and properties of polypseudouridylic acid
Irving H. Goldberg
Pages 519-521

[136] Enzymatic synthesis of polyguanylic acid and copolymers containing guanylic
acid
M.N. Thang, M. Grunberg-Manago
Pages 522-529

[137] Phage Qβ RNA polymerase
Lillian Eoyang, J.T. August
Pages 530-540

[138] The preparation of an RNA replicase capable of synthesizing biologically
active viral RNA
N.R. Pace, I. Haruna, S. Spiegelman
Pages 540-555

[139] RNA polymerase (ribonucleotide triphosphate-ribonucleic acid
ribonucleotidyl transferase)
Samuel B. Weiss
Pages 555-565

[140] The isolation and properties of the DNA-dependent RNA polymerase from
Azotobacter vinelandii
Joseph S. Krakow, William J. Horsley
Pages 566-571

[141a] RNA polymerase in animal cells infected with RNA viruses
Richard M. Franklin
Pages 572-576

[141b] Soluble RNA-dependent nucleotide-incorporating activity
D.P. Burma
Pages 576-579

[141c] tRNA … pCpCpA pyrophosphorylase of rat liver
Violet Daniel, Uriel Z. Littauer
Pages 579-585

[142] DNA polymerase from T2-infected Escherichia coli
H. Vasken Aposhian
Pages 586-591

[143] Deoxynucleotide polymerizing enzymes from calf thymus gland
F.J. Bollum
Pages 591-611

[144] Demonstration of DNA polymerase in vivo
F.J. Bollum
Pages 615-616

[145] The measurement of bacterial DNA synthesis and segregation by means of
autoradiography
Cynthia Lark
Pages 616-625

[146] Measurement of DNA synthesis in bacterial cells
J.D. Friesen
Pages 625-635

[147] The DNA-agar procedure
Arnold J. Bendich, Ellis T. Bolton
Pages 635-640

[148] The formation and detection of DNA-RNA hybrids
D. Gillespie
Pages 641-668
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Previously published articles from Methods in Enzymology related to Section XI
Page 670
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[149] RNase hydrolysis of aminoacyl-sRNA to aminoacyl adenosine
George Acs, Fritz Lipmann
Pages 671-675

[150a] Reduction of aminoacyl-sRNA and peptidyl-sRNA compounds to the
corresponding amino alcohol derivatives
Alton Meister
Pages 675-681

[150b] N10-formyl tetrahydrofolate:methionyl tRNA(F) transformylase from
Esherichia coli B
Herbert W. Dickerman, Herbert Weissbach
Pages 681-687

[151] Determination of adenosine- and aminoacyl adenosine-terminated sRNA
chains by ion-exclusion chromatography
Richard Wolfenden
Pages 687-691

[152] Techniques for measuring specific sRNA binding to Escherichia coli ribosomes
Akira Kaji
Pages 692-699

[153] Aminoacyl transfer enzymes from reticulocytes
Ralph Arlinghaus, Roger Heintz, Richard Schweet
Pages 700-708

[154] Isolation and properties of the Escherichia coli amino acid polymerizing
enzymes
Yasutomi Nishizuka, Fritz Lipmann, Jean Lucas-Lenard
Pages 708-721

[155] Aminoacyl transfer to ribosomal protein in the rat liver system
Kivie Moldave
Pages 721-725

[156] An assay for the binding of aminoacyl-tRNA to ribosomes
Philip Leder
Pages 725-727

[157a] Isolation and properties of the reticulocyte protein synthesizing system
coded by RNA and by a sucrose gradient fraction of RNA
Jacques Kruh
Pages 728-747

[157b] Methods of identification of peptides during hemoglobin biosynthesis and
measurement of their sequential synthesis
G. Schapira, J. Rosa, N. Maleknia, P. Padieu
Pages 747-769

[158] Coding behavior in the Escherichia coli in vitro protein-synthesizing system of
fractions of bacterial RNA isolated by sucrose gradient centrifugation or by
chromatography on methylated albumin-kieselguhr columns
Donald H. Hayes, François Gros
Pages 770-782

[159] Description of nucleic acid coding for mammalian protein synthesis
I. Bernard Weinstein
Pages 782-787

[160] Natural RNA coding of bacterial protein synthesis
Daniel Nathans
Pages 787-791

[161] Demonstration of DNA-dependent amino acid incorporation
Audrey Stevens
Pages 791-794

[162] Techniques for demonstrating DNA-dependent protein synthesis
B. Nisman
Pages 794-820

[163a] DNA-mediated amino acid incorporation
B.J. McCarthy
Pages 820-823

[163b] Amino acid incorporation mediated by ribosomal RNA and soluble RNA
B.J. McCarthy
Pages 824-825

[164] Characterization of the polypeptidyl-sRNA complex in Escherichia coli cell-free
systems
M.S. Bretscher
Pages 825-831

[165] Techniques for the release of nascent protein from ribosomes
Allan J. Morris
Pages 831-837

[165a] Enzymatic synthesis of trinucleoside diphosphates of known sequence
Philip Leder
Pages 837-839

[166] Preparation of cells of Escherichia coli with altered permeability
Leon A. Heppel
Pages 841-846

[167] Procedures for Bacillus subtilis transformation
Inga Mahler
Pages 846-850

[168] Spheroplast assay of φX174 DNA
Robert L. Sinsheimer
Pages 850-858

[169] Genetic transformation of bacteriophage T4
Gerrit Veldhuisen, Edward B. Goldberg
Pages 858-864

[170] Procedures for Hemophilus influenzae transformation
Sol H. Goodgal
Pages 864-876

[171] On the infection of bacteria with λDNA—the helper-infected system
A.D. Kaiser
Pages 877-880

[172] Assay of M12 phage RNA infectivity in spheroplasts
P.H. Hofschneider, H. Delius
Pages 880-886

[173] Purine- and pyrimidine-protein conjugates
Sam M. Beiser, Stuart W. Tanenbaum, Bernard F. Erlanger
Pages 889-893

[174] Preparation and assay of nucleic acids as antigens
Otto J. Plescia
Pages 893-899

[175] Preparation of nucleoside-specific synthetic antigens
Michael Sela, Hanna Ungar-Waron
Pages 900-903

[176] Immunological detection of ribonucleic acids by Agar diffusion
F. Lacour
Pages 903-907
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Methods in Enzymology

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Nucleic Acids, Part B

Lawrence Grossman, Kivie Moldave

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