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Methods in Enzymology

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Nucleic Acids, Part A

Lawrence Grossman, Kivie Moldave


Volume 12, Part A,
Pages 2-764 (1967)

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Contributors to volume XII, Part A
Pages v-viii
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Preface
Lawrence Grossman, Kivie Moldave
Page ix

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Previous volumes
Page xviii

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Volumes in preparation
Pages xix-xx

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Previously published articles from Methods in Enzymology related to section I
Pages 2-3

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[1] Xanthine oxidase from bovine small intestine
G.G. Roussos
Pages 5-16

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[2] Synthesis of aminoacyl-N10 adenines
A.H. Schein, J. Brink
Pages 16-19

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[3] Isolation of thymine dimers
Helge L. Günther, William H. Prusoff
Pages 19-30

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[4] The reaction of hydrazine with pyrimidine bases
D.M. Brown
Pages 31-34

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[5] The reaction of hydroxylamine with pyrimidine bases
John H. Phillips
Pages 34-38

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[6] Reduction of pyrimidines
Arnold H. Schein, Florence T. Schein
Pages 38-45

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[7] Photosensitized oxidation of purines and pyrimidines
Melvin Simon
Pages 45-47

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[8] Thymine 7-hydroxylase
Mitchel T. Abbott
Pages 47-50

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[9] Uracil and thymine reductases
D. Hunninghake, Santiago Grisolia
Pages 50-59

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[10] Techniques for total synthesis of nucleosides
Iris Wempen, Jack J. Fox
Pages 59-76

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[11] Synthesis of nucleoside derivatives by conversion from preformed nucleosides
Iris Wempen, Jack J. Fox
Pages 76-93

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[12] The use of the borate complex
J.X. Khym
Pages 93-101

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[13] The isolation and characterization of pseudouridine
Waldo E. Cohn
Pages 101-108

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[14] The thiobarbituric acid method for deoxyribonucleosides
L.D. Saslaw, V.S. Waravdekar
Pages 108-113

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[15] Purine nucleoside phosphorylases: (Purine-nucleoside: orthophosphate
ribosyltransferases EC2.3.2.1) Purine + R1-P(sR1-P) ⇌ ribonucleotides
(deoxyribonucleosides) + Pi
H.L.A. Tarr
Pages 113-118

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[16] Pyrimidine nucleoside and deoxynucleoside phosphorylases
W.E. Razzell
Pages 118-125

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[17] A purified nonspecific adenosine deaminase from taka-diastase
T.K. Sharpless, Richard Wolfenden
Pages 126-131

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[18] Ribonucleoside cyclic phosphates
D. Shugar
Pages 131-137

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[19] Isolation and characterization of dihydrouridylic acid from RNA
James T. Madison
Pages 137-140

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[20] Chemical methylation of nucleic acid components
Beverly E. Griffin
Pages 141-155

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[21] Mammalian ribonucleoside diphosphate reductases
E.Colleen Moore
Pages 155-164

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[22] Adenylate deaminase from calf brain
Barbara Setlow, J.M. Lowenstein
Pages 164-169

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[23] Deoxycytidylate deaminase
Frank Maley
Pages 170-182
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[24a] Chemical syntheses of specifically α-, β-, and γ-32P-labeled nucleoside
5′-triphosphates
J.G. Moffatt
Pages 182-192

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[24b] Biosynthetic preparation of 32P-labeled nucleoside 5′-phosphates and
derivatives
Robert B. Hurlbert, N.Burr Furlong
Pages 193-202

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Previously published articles from Methods in Enzymology related to section II
Page 204

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[25] The preparation of pyrimidine oligonucleotides by acid hydrolysis of DNA
Herman S. Shapiro
Pages 205-212

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[26] The preparation of purine oligonucleotides by hydrazinolysis of DNA
Herman S. Shapiro
Pages 212-218

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[27] Controlled partial hydrolysis of RNA
Robert M. Bock
Pages 218-221

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[28] Preparation of apurinic acid and of oligodeoxyribonucleotides with formic acid
and diphenylamine
K. Burton
Pages 222-224

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[29] Alkaline hydrolysis of RNA
R.M. Bock
Pages 224-228

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[30a] Ribonuclease T1 from Taka-Diastase
Tsuneko Uchida, Fujio Egami
Pages 228-239

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[30b] Ribonuclease T2 from Taka-Diastase
Tsuneko Uchida, Fujio Egami
Pages 239-247

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[31] An endonuclease from Neurospora crassa specific for polynucleotides lacking an
ordered structure
Stuart Linn
Pages 247-255

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[32] Preparation of oligoribonucleotides with RNase I
W.E. Razzell
Pages 255-257

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[33] Micrococcal nuclease
K.K. Reddi
Pages 257-262

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[34a] An exonuclease induced by bacteriophage lambda
John W. Little
Pages 263-269

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[34b] Preparation of Mustelus canis liver deoxyribonuclease
Hilary Ashe, Lawrence Levine
Pages 270-273

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[35] Radioactivity detection—Continuous counting
J.A. Hunt
Pages 273-281

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[36] Paper chromatography and characterization of oligodeoxyribonucleotides
M. Laskowski
Pages 281-304

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[37] Partition chromatography of nucleic acid components (Isolation of the minor
nucleosides)
Ross H. Hall
Pages 305-315

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[38a] Techniques for elution of nucleic acid components from paper
Leon A. Heppel
Pages 316-317

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[38b] Ultraviolet photography of paper-chromatographed nucleic acid components
Leon A. Heppel
Pages 317-318

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[39] Chromatographic characterization of oligonucleotides on DEAE paper
N.Burr Furlong
Pages 318-323

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[40] Thin-layer separation methods for nucleic acid derivatives
Kurt Randerath, Erika Randerath
Pages 323-347

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[41] Ion-exchange paper techniques for assay of nucleotide pyrophosphorylase
Jesse F. Scott
Pages 348-350

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[42] Paper electrophoresis of nucleic acid components
J.D. Smith
Pages 350-361

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[43] A two-dimensional fractionation method for radioactive nucleotides
F. Sanger, G.G. Brownlee
Pages 361-381

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[44] Chromatography of nucleic acid components on Dowex 1 columns by the use
of an automatic recording system
Masatake Hori
Pages 381-390
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[45] Dowex 1 chromatography of oligonucleotides
Kin-ichiro Miura, Yutsuki Hayashi
Pages 390-395

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[46] Mapping of oligonucleotides
George W. Rushizky
Pages 395-398

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[47] Ion-exchange chromatography in the presence of urea
G.M. Tener
Pages 398-404

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[48] Fractionation of oligoribonucleotides according to degree of polymerization
Wendell M. Stanley
Pages 404-407

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[49] Desalting of nucleotides by gel filtration
Mayo Uziel
Pages 407-414

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Previously published articles from Methods in Enzymology related to section III
Page 416

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[50] The isolation and purification of mammalian cell nuclei
Tung Yue Wang
Pages 417-421

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[51] Isolation and purification of nuclei
Harris Busch
Pages 421-448

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[52] Isolation and purification of nucleoli
Harris Busch
Pages 448-464

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[53] Preparation of mitochondrial fractions from Neurospora crassa
David J.L. Luck
Pages 465-468

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[54] The isolation and purification of yeast mitochondria
D.W. Gregory, E.S. Polakis, W. Bartley
Pages 469-476

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[55] Isolation of chloroplasts from Euglena gracilis
Jerome M. Eisenstadt, George Brawerman
Pages 476-478

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[56a] Purification of mammalian ribosomes
Kivie Moldave, Lawrence Skogerson
Pages 478-481

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[56b] Preparation of polysomes from mammalian reticulocytes, lymph nodes, and
cells grown in tissue culture
Alexander Rich
Pages 481-491

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[57] Preparation of ribosomes and their subunits from Escherichia coli cells
M. Takanami
Pages 491-494

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[58] Purification of ribosomes from Euglena gracilis
Jerome M. Eisenstadt, George Brawerman
Pages 494-498
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[59] Resolution and isolation of yeast polysomes
Leon Marcus, Harlyn O. Halvorson
Pages 498-502

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[60] A technique of rapid lysis for the preparation of Escherichia coli polyribosomes
G.N. Godson
Pages 503-516

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[61] Preparation of polyribosomes from Bacillus megaterium KM
Moselio Schaechter
Pages 516-520

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[62] Enzymatic method for the isolation of polyribosomes from Escherichia coli
Marc H. Dresden
Pages 520-523

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[63] Chromatographic purification of ribosomes
Wendell M. Stanley, Albert J. Wahba
Pages 524-526

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[64] Preparation of the soluble fraction from bacteria
M. Lamborg
Pages 527-529

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Previously published articles from Methods in Enzymology related to section IV
Page 532

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[65] The isolation of DNA from mitochondria
George F. Kalf, Mary Ann Gréce
Pages 533-538

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[66] The isolation of DNA from yeast
D. Smith, H.O. Halvorson
Pages 538-541

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[67] Isolation of DNA from chloroplasts
Jerome M. Eisenstadt, George Brawerman
Pages 541-543

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[68] Preparation of bacterial DNA by the phenol-pH 9-RNases method
Kin-ichiro Miura
Pages 543-545

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[69] Isolation of high molecular weight DNA from normal and phage-infected
Escherichia coli
Mervyn G. Smith
Pages 545-550

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[70] The use of NaClO4 to isolate bacteriophage nucleic acids
David Freifelder
Pages 550-554

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[71] Isolation from crabs of deoxyribonucleate with alternating deoxyadenylate and
thymidylate residues
Robert P. Klett, Michael Smith
Pages 554-557

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[72] The isolation and characterization by sucrose gradients of DNA from phage-
infected bacterial cells
Hamilton O. Smith, Myron Levine
Pages 557-562

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[73] Fractionation of DNA on methylated albumin column
Noboru Sueoka, Ts'ai-ying Cheng
Pages 562-566

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[74] Fractionation of nucleic acids by dextran-polyethylene glycol two-phase
systems
Bruce M. Alberts
Pages 566-581

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[75] Isolation of ribonucleic acids from mammalian cells and animal viruses
Marc Girard
Pages 581-588

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[76] Isolation of sRNA from intact Escherichia coli cells
Gunter von Ehrenstein
Pages 588-596

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[77] Isolation of sRNA from intact yeast cells
Robert W. Holley
Pages 596-598

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[78] Purification of aminoacyl-sRNA by molecular sieve chromatography on
Sephadex
Kivie Moldave, Richard P. Sutter
Pages 598-601

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[79] Preparation of highly purified sRNA from yeast
Tomas Lindahl, Jacques R. Fresco
Pages 601-607

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[80] Preparation of RNA from mammalian ribosomes
Kivie Moldave
Pages 607-608

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[81] Preparation of RNA from rabbit reticulocytes and liver
Jacques Kruh
Pages 609-613

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[82] Isolation of RNA phage M12 replicative form RNA
P.H. Hofschneider, J. Ammann, B. Francke
Pages 613-620

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[83] The fractionation of RNA's by sucrose gradient centrifugation
Edwin H. McConkey
Pages 620-634
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[84] Fractionation of RNA's on MAK columns
W.T. Murakami
Pages 634-637

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[85] Chromatography of sRNA on DEAE-cellulose and DEAE-Sephadex
Robert M. Bock, Joseph D. Cherayil
Pages 638-644

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[86] Fractionation of RNA's by countercurrent distribution
B.P. Doctor
Pages 644-657

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[87] Fractionation of aminoacyl-sRNA on methylated albumin column
Noboru Sueoka, Tetsuo Yamane
Pages 658-660

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