TUTORIAL 1

2.a)
Time (h) X (g/L) S (g/L)
Growth Profile
10
0 0.2 9.23 9
2 0.211 9.21 8

concentration (g/L)
4 0.605 9.07 7
8 0.908 8.03 6
5
10 1.77 6.8
4
12 3.2 4.36 3
14 5.6 0.92 2
16 6.15 0.077 1
18 6.2 0 0
0 2 4 6 8 10 12 14 16 18 20
20 6.18 0
time (h)

b
Time
X (g/L) S (g/L) ln (Xt) ln (Xt/Xo)
(h)
0 0.2 9.23 -1.60944 0
0.05354
2 0.211 9.21
-1.5559 1
1.10691
4 0.605 9.07
-0.50253 1
1.51292
8 0.908 8.03
-0.09651 7
2.18041
10 1.77 6.8
0.57098 7
1.16315 2.77258
12 3.2 4.36
1 9
1.72276 3.33220
14 5.6 0.92
7 5
1.81645
16 6.15 0.077
2 3.42589
1.82454 3.43398
18 6.2 0
9 7
1.82131 3.43075
20 6.18 0
8 6

Method 1:
ln X t=ln X o + μt
 ln Xt vs t
2.5
2
f(x) = 0.238110271214204 x − 1.79955196157496
1.5
1
Xt (g/L)

0.5
0
0 2 4 6 8 10 12 14 16 18
-0.5
-1
-1.5
-2
time (h)

ln X t
=μt
ln X o

ln (Xt/Xo) vs t
4
3.5 f(x) = 0.238110271214204 x − 0.190114049140864
3
ln(Xt/Xo) (g/L)

2.5
2
1.5
1
0.5
0
0 2 4 6 8 10 12 14 16 18
t (h)

−1
specific growth rate , μ=0.2381 h
c)
ln 2 ln 2
t d= = =2.911 h
μ 0.2381
d)
X m− X i 6.2−0.2 gL
productivity of cell= = =0.33
tm 18 hr
X m−X i 6.2−0.2 g of cell produced
y X / S= = =0.65
S i−S m 9.23−0 g of substrate consumed

e)
 ( )
X f = X i +S i y X ¿ 0.2+20 ( 0.65 )¿ 13.2 g / L
S

Q3.a)
Note: set y-intercept to 0 in excel

Standard Calibration Curve

350

300
f(x) = 9.80464646464646 x
250

200
Peak area

150

100

50

0
0 5 10 15 20 25 30 35
Glucose concentration (g/L)

b)
y=9.8046 x
Where y is the peak area and x is the glucose concentration

c)
to draw orange line:
x y
0 209.86
21.4042
209.86
4
21.4042 0
 Standard Calibration Curve
350
300
f(x) = 9.80464646464646 x
250
Peak area 200
150
100
50
0
0 5 10 15 20 25 30 35
Glucose concentration (g/L)

209.86=9.8046 x
g
x=21.4042
L
4)
time (min) spore density (m-3) ln x
0 8.50E+10 25.16592
5 4.23E+09 22.16547
10 6.20E+07 17.94264
15 1.80E+06 14.4033
20 4.50E+04 10.71442
30 3.25E+01 3.48124

graph of ln X vs t
30

25
f(x) = − 0.72232721504273 x + 25.3696460306972
ln X (m-3)

20

15

10

0
0 2 4 6 8 10 12
t (min)