M0, M1 and M2 differentiation markers in THP1

M0

hCD68 (verified)
Forward: TAGCTGGACTTTGGGTGAGG
Reverse: GTGGTTTTGTGGCTCTTGGT

hCD71 (verified)
Forward: ATCGGTTGGTGCCACTGAATGG
Reverse: ACAACAGTGGGCTGGCAGAAAC

hCD36 (verified)
Forward: CAGGTCAACCTATTGGTCAAGCC
Reverse: GCCTTCTCATCACCAATGGTCC

hCD14 (verified) (this is the only markers that should decrease its expression)
Forward: CTGGAACAGGTGCCTAAAGGAC
Reverse: GTCCAGTGTCAGGTTATCCACC

M1

hTNFa (verified)
Forward: CTCTTCTGCCTGCTGCACTTTG
Reverse: ATGGGCTACAGGCTTGTCACTC

hIL-1b (verified)
Forward: CCACAGACCTTCCAGGAGAATG
Reverse: GTGCAGTTCAGTGATCGTACAGG

hIL6 (verified)
Forward: AGACAGCCACTCACCTCTTCAG
Reverse: TTCTGCCAGTGCCTCTTTGCTG

hCXCL10 (verified)
Forward: GGTGAGAAGAGATGTCTGAATCC
Reverse: GTCCATCCTTGGAAGCACTGCA

hCD80(verified)
Forward: CTCTTGGTGCTGGCTGGTCTTT
Reverse: GCCAGTAGATGCGAGTTTGTGC

hHDLA-DRB1 (verified)
Forward: GAGCAAGATGCTGAGTGGAGTC
Reverse: CTGTTGGCTGAAGTCCAGAGTG
M2

hCD206 (MRC1) (verified)

Forward: AGCCAACACCAGCTCCTCAAGA
Reverse: CAAAACGCTCGCGCATTGTCCA

hCD163 (verified)
Forward: GGATCTGCTGACTTCAGAAG
Reverse: CTCCTTGTCTGTTCCTCCAA

hFibronectin fibronectin 1 (FN1) (verified)

Forward: ACAACACCGAGGTGACTGAGAC
Reverse: GGACACAACGATGCTTCCTGAG

hIL10 (verified)
Forward: TCTCCGAGATGCCTTCAGCAGA
Reverse: TCAGACAAGGCTTGGCAACCCA

hCCL18 (verified)
Forward: TGGCAGATTCCACAAAAGTTCA
Reverse: GGATGACACCTGGCTTGGG

hCCL22 (verified)
Forward: GAGCATGGATCGCCTACAG
Reverse: CAGACGGTAACGGACGTAATC

Housekeeping

hGAPDH (verified)
Forward: GTCTCCTCTGACTTCAACAGCG
Reverse: ACCACCCTGTTGCTGTAGCCAA

hribosomal protein S18 (RPS18), mRNA (verified)

Forward: GCAGAATCCACGCCAGTACAAG
Reverse: GCTTGTTGTCCAGACCATTGGC
M0, M1 and M2 differentiation markers

M0

mCD68 (verified)
Forward: GGCGGTGGAATACAATGTGTCC
Reverse: AGCAGGTCAAGGTGAACAGCTG

mCD71  (verified)
Forward: GAAGTCCAGTGTGGGAACAGGT
Reverse: CAACCACTCAGTGGCACCAACA

mCD36 (verified)
Forward: GGACATTGAGATTCTTTTCCTCTG
Reverse: GCAAAGGCATTGGCTGGAAGAAC

mCD14 (verified)
Forward: TTGAACCTCCGCAACGTGTCGT
Reverse: CGCAGGAAAAGTTGAGCGAGTG

M1

mTNFa (verified)
Forward: GGTGCCTATGTCTCAGCCTCTT
Reverse: GCCATAGAACTGATGAGAGGGAG

mIL-1b (verified)
Forward: TGGACCTTCCAGGATGAGGACA
Reverse: GTTCATCTCGGAGCCTGTAGTG

mIL6 (verified)
Forward: TACCACTTCACAAGTCGGAGGC
Reverse: CTGCAAGTGCATCATCGTTGTTC

mCXCL10 (verified)
Forward: ATCATCCCTGCGAGCCTATCCT
Reverse: GACCTTTTTTGGCTAAACGCTTTC

mCD80 (verified)
Forward: CCTCAAGTTTCCATGTCCAAGGC
Reverse: GAGGAGAGTTGTAACGGCAAGG
M2

mCD206 (MRC1) (verified)

Forward: GTTCACCTGGAGTGATGGTTCTC
Reverse: AGGACATGCCAGGGTCACCTTT

mCD163 (verified)
Forward: GGCTAGACGAAGTCATCTGCAC
Reverse: CTTCGTTGGTCAGCCTCAGAGA

mFibronectin (verified)
Forward: CCCTATCTCTGATACCGTTGTCC
Reverse: TGCCGCAACTACTGTGATTCGG

mIL10 (verified)
Forward: CGGGAAGACAATAACTGCACCC
Reverse: CGGTTAGCAGTATGTTGTCCAGC

mCCL22 (verified)
Forward GGCACCTATCCAGTGCCACA
Reverse: TGGTGGACCAGCCTGAAACTC

Housekeeping

mGAPDH (verified)
Forward: CATCACTGCCACCCAGAAGACTG
Reverse: ATGCCAGTGAGCTTCCCGTTCAG

hribosomal protein S18 (RPS18), mRNA (verified)

Forward: CGGAAAATAGCCTTCGCCATCAC
Reverse: ATCACTCGCTCCACCTCATCCT