KRIS SAMANTHA B.

ROCHE BSMLS-1A

10 WAYS TO MAKE LABORATORY ACTIIVITIES SUCCESSFUL

1. Specify your objective.
2. Discover the most effective method for conducting successful experiments.
3. Formulate the protocol.
4. Obtain all necessary reagents ahead of time and ensure that they are in good
condition.
5. Prepare a timeline.
6. Improve the lighting in your workspace.
7. Carry out the experiment correctly.
8. Limit distractions.
9. Keep a detailed record of everything.
10. Analyze the results.

TYPES OF PIPETTES AND THEIR USES

1. Volumetric Pipettes
Volumetric pipettes are used by those investigating chemical properties and analyzing
reactions. Most schools, universities, and professional laboratories have them. They are
known for their precision and can measure up to four significant figures. Researchers
can use them to measure the volume of a concentrated stock solution because they
come in a variety of sizes.
2. Graduated Pipettes
Graduated pipettes are less accurate than volumetric pipettes. Serological graduated
pipettes, also known as "blow out pipettes," do not have zero marks at the start of their
conical end. Mohr graduated pipettes, also known as "drain out pipettes," have zero
marks at the start of their conical end.
3. Vacuum-assisted Pipettes
Vacuum-assisted pipettes may be graduated or volumetric. Graduated vacuum-assisted
pipettes have multiple graduation marks, whereas volumetric vacuum-assisted pipettes
only have one graduation mark because it typically measures a single volume.
Polystyrene, glass, and borosilicate are used to make vacuum-assisted pipettes. They
need a suction device, but they don't have pistons.
4. Micropipettes
Scientists and technicians can use micropipettes to take extremely precise
measurements. Calibration of micropipettes should be done on a regular basis, at least
once every 3-6 months.
5. Pasteur Pipettes
KRIS SAMANTHA B. ROCHE BSMLS-1A

Glass pipettes are used in the pasteurization process. A Pasteur pipette resembles a
classic liquid dropper because of its bulb-shaped top. Today, Pasteur pipettes are
thought to be fairly imprecise. They aren't calibrated or graded, and they're more
commonly employed to transfer aqueous solutions from one container to another in
biology laboratories than in chemical laboratories. Pasteur pipettes, named after the
French physician Louis Pasteur, are frequently discarded after usage.

GUIDELINES OF PROPER PIPETTING

1. Set the volume on the pipette.
2. Depress the plunger.
3. Allow the plunger to smoothly return to its resting position after immersing the tip
to the correct depth, which varies depending on the pipette and tip.
4. Wait for the liquid to flow into the tip for about a second.
5. Place the pipette against the receiving chamber's wall, holding it at 10–45
degrees, and smoothly depress the plunger to the first stop.
6. Depress the plunger to the second stop after one second.
7. To remove the pipette, slide the tip up the vessel wall.
8. Return the plunger to its resting position.