LC that takes your

productivity to
new heights

The collective power

of chromatography

List of compendial methods

Thermo Scientific Charged Aerosol Detectors
Charged aerosol detection is a reliable technology that will change the way
you view every sample. Charged Aerosol Detector (CAD) can detect all
non-volatile, and many semi-volatile analytes, with uniform response.
Charged aerosol detection can be used for the analysis of pharmaceuticals
(large and small molecule), biomolecules, foods and beverages, specialty
chemicals, and polymers.

The following section gives examples of standardized methods - e.g.

United States Pharmacopeia (USP), European Pharmacopeia (EP), and
International Organization for Standardization (ISO) - using charged aerosol
detection technology.
 Gradient
Method Additional
Analytes Organization Matrix Column Mobile Phase or Status
Reference Information
Isocratic

Triton X-100, ISO 18254-2:2018

Normal phase (NP)
octylphenol Method for the
applying two columns: A) acetonitrile with
ethoxylates detection and
1) C18 column, 4.6 x 0.1% formic acid AppsLab Library
(CAS 9002-93-1) determination
ISO Textiles 50 mm, 1.7 µm B) methanol with 0.1% Gradient In official text
IGEPAL CO-630, of alkylphenol
2) hydrogen bond formic acid and 0.01%
nonylphenol ethoxylates (APEO) -
adsorption, 4.6 x 150 ammonium formate
ethoxylates Part 2: Method using
mm, 3 µm
(CAS 68412-54-4) NPLC

ISO 16560:2015(en) Nonionic

Surface active agents ethoxylated
PEG with — Determination surfactants that
Reversed phase (RP)
molecular mass of polyethylene are soluble in A) water Surfactants Application
ISO C18, 4.6 x 250 mm, Gradient In official text
greater than glycol content in methanol or B) methanol Notebook
5 µm
400 g/mol nonionic ethoxylated methanol/water/
surfactants — HPLC H2O and have
method [PEG]>0.1%

Type L1, Thermo

Deoxycholic A) 0.1% formic acid
Scientific™ Acclaim™ AppsLab
Deoxycholic acid (desoxycholic) acid in water
USP None 120 C18, Gradient In official text
powder - since USP 40 NF B) 0.1% formic acid in Application Note
4.6 × 150 mm, 3 μm
35 S1 acetonitrile
(P/N 059133)

Content of metoprolol
related compound
H and metoprolol
Hydrophilic interaction
related compound I,
liquid chromatography
USP 41(3) In-Process 85% acetonitrile
(HILIC) In process Application Note
Metoprolol Revision: 15% 0.1 M ammonium
USP None solid core silica gel Isocratic / under
succinate powder Metoprolol succinate. formate in water, AppsLab Library
with five hydroxyl consideration
Proposed change pH 3.2
bonded ligands,
to United States
4.6 x 150 mm, 5 µm
Pharmacopeia and
National Formulary
USP 38-NF33;

A) 0.5% acetonitrile
Gadobutrol RP end-capped
and 99.5% water pH
Gadobutrol Monohydrate – Ph. phenylhexylsilyl silica
EP None 3.6 adjusted with Gradient In official text
monohydrate Eur. 9.0 gel, 4.6 x 250 mm,
formic acid
07/2016:1215 3 µm
B) acetonitrile

19.5% methanol,
RP end-capped solid
Vigabatrin 80.5% water
core phenylhexylsilyl Adopted for
Vigabatrin Pharmeuropa 30.2, EP None (v/v) with 2.1 g/L Isocratic
silica gel, 4.6 x 100 next version
April 2018. perfluoroheptanoic
mm, 2.7 µm
acid (PFHPA)
 Gradient
Method Additional
Analytes Organization Matrix Column Mobile Phase or Status
Reference Information
Isocratic

RP solid core
Topiramate A) 1.93 g/L ammonium
pentafluorophenylsilyl Adopted for
Topiramate Pharmeuropa 27.4, EP None acetate, pH 3.5 Gradient
silica gel, 4.6 x 100 next version
October 2015. B) acetonitrile
mm, 2.6 µm

A) water
RP octadecylsilyl silica
B) 150 mL acetonitrile
Ibandronate Ibandronate gel with embedded In process
plus 150 mL of
sodium Pharmeuropa 22.4, EP None strong anion exchange Gradient / under
11.4 g/L trifluoroacetic
monohydrate October 2010. groups, 4.6 x 150 mm, consideration
acid, filled to 1 L with
2.7 µm)
water

4% 0.36 g/L already

Aspartic acid defined above acid In process
RP C18, 4.6 x 150 mm,
Aspartic acid Pharmeuropa 22.4, EP None (PFHPA) in methanol Isocratic / under AppsLab Library
5 µm
October 2010. 96% 0.36 g/L PFHPA consideration
in water (%/% v/v)

Validation and
application of an
HPLC-CAD-TOF/ A) 0.2 M ammonium
Mixed mode
MS method for formate in water AppsLab Library
Thermo Scientific™
API and identification and (pH4.0) Published for
EP None Acclaim™ Trinity P1, 3.0 Gradient
counterions quantification of B) water consideration Mixed-mode HPLC
x 50 mm, 3 µm (P/N columns
pharmaceutical C) acetonitrile
071388)
counterions.
Pharmeuropa bio & scientific
notes 2014, 81-91.

Determination of
the purity of valine
by isocratic liquid 20 mM
Valine and chromatography RP C18, 4.0 x 150 mm, Perfluorobutyric acid Published for AppsLab Library
EP None Isocratic
impurities coupled with charged 3 µm in acetonitrile/water consideration
aerosol detection (10:90 v/v)
Pharmeuropa bio & scientific
notes. 2015; 2015, 11-18.

For more references download the Charged Aerosol Detection bibliography highlighting the breadth and scope of different analytical methods
found in the literature: https://www.thermofisher.com/de/de/home/global/forms/industrial/charged-aerosol-detection-bibliography.html

Find out more at thermofisher.com/cad

© 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and
its subsidiaries unless otherwise specified. This information is presented as an example of the capabilities of Thermo
Fisher Scientific Inc. products. It is not intended to encourage use of these products in any manners that might infringe the
intellectual property rights of others. FL73052-EN 0619S