EXPERIMENT 1

SPECTROPHOTOMETRY

DATA SHEET

Date ___________________ Score ____________

Group No. ______________

STUDENT ID

Leader _______________________ _________________________

_______________________ _________________________

_______________________ _________________________

_______________________ _________________________

I. COLOR OF VISIBLE RADIATION

WAVELENGTH COLOR WAVELENGTH COLOR

400 650
450 700
500 750
550 800
600  

II. VISIBLE SPECTRUM OF SAMPLE NAME (CONCENTRATION)

WAVELENGTH ABSORBANCE WAVELENGTH ABSORBANCE WAVELENGTH ABSORBANCE

380 520 670
400 530 680
410   540 690
420 550 700
430   560 710
440 570   720  
450 580 730
460 590 740
 470 600 750
480 620 760
490 640 770
500 650 780

III. DETERMINATION OF CONCENTRATION USING ABSORBANCE

VALUES

WAVELENGTH

SAMPLE NAME
CONCENTRATION (uM) CONCENTRATION (PPM) ABSORBANCE

APPROVED BY: ____________________

PROFESSOR

GRAPHS (CONCENTRATION, WAVELENGTH)

 A

or
ba
bs

0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%

37
0
39
0
41
0
43
0
45
0
47
0
49
0
51
0
53
0
55
0
57
0
59
0
BLUE DYE

61
0
63
0
65
0
67
0
69
0
71
0
73
0
75
0
77
0
79
0