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1 Introducing biology

6 Introducing
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biology

Think about…
1.1 What is biology?
1.2 How can we study biology?
1.3 Nature of science
Recall Think about…
Concept map

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biology

Sophia the robot

• A robot equipped with


artificial intelligence
• Able to
 recognize faces
 understand what people say
 give a reply
 walk
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biology

Sophia the robot


• Biologists do not consider her a
human or an organism.

Human /
Organism

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biology

Which characteristics of organisms


does Sophia have?

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Why do biologists not consider


Sophia an organism?

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biology 1.1 What is biology?

1.1 What is biology?

Biology is the scientific


study of organisms.

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biology 1.1 What is biology?

1.1 What is biology?


Organism’s Organism’s Organism’s
structures life cycles classification

Examples of areas
of study in biology

Organism’s interactions with other


organisms and their environment
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biology 1.1 What is biology?

A Characteristics of organisms
characteristics of organisms

Organisms carry out vital functions


necessary for maintaining life.

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biology 1.1 What is biology?

A Characteristics of organisms
Organism Non-living things

Distinguished based on
characteristics of organisms
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biology 1.1 What is biology?

A Characteristics of organisms
1 Reproduce and grow
Reproduction
• produce
offspring

new rice plants


are reproduced
through seeds
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biology 1.1 What is biology?

A Characteristics of organisms
1 Reproduce and grow
The offspring grow in size and often
in complexity

offspring will
grow and
reproduce
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biology 1.1 What is biology?

A Characteristics of organisms
2 Obtain food and respire

Organisms obtain food in the


process of nutrition.
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biology 1.1 What is biology?

A Characteristics of organisms
2 Obtain food and respire

Plants
make their own food by photosynthesis
(*autotrophic nutrition) 14
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biology 1.1 What is biology?

A Characteristics of organisms
2 Obtain food and respire

Animals
obtain food by feeding
(*heterotrophic nutrition) 15
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A Characteristics of organisms
2 Obtain food and respire
For growth and repair

Food Broken down to release energy


through respiration
for carrying out
vital functions
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A Characteristics of organisms
3 Detect and response to stimuli,
and move detect stimuli
(e.g. a lion sees its prey)

make response
(may involve
movement)
Irritability
(e.g. it runs after its prey)
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A Characteristics of organisms
4 Excrete to maintain a relatively
constant internal environment
• Excretion: removal
of metabolic wastes
from the body
(harmful at high conc.)
carbon
dioxide urea
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biology 1.1 What is biology?

A Characteristics of organisms
4 Excrete to maintain a relatively
constant internal environment
• the maintenance of a relatively
constant internal environment in an
organism (e.g. body temperature) is
called homeostasis

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biology 1.1 What is biology?

B Importance of studying
biology
1 Develop appreciation of and respect
for all organisms and the environment

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biology 1.1 What is biology?

B Importance of studying
biology
2 Develop our ability to think and solve
problems scientifically
Is it true?
Special lights in our Has any fair test
refrigerator can help been done?
increase the vitamin C
content in vegetables.

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B Importance of studying
biology
3 Better understand health issues,
environmental issues and social issues.

Are electronic cigarettes Should our country parks


less harmful than regular be developed for residential
cigarettes? purposes to ease our
housing problem?
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Are viruses organisms?


• Viruses can reproduce after entering
living cells (hosts). But without living
cells, viruses do not show any
characteristics of organisms.
• It is hard to define whether viruses are
living or non-living.

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biology 1.1 What is biology?

1 Biology is the scientific study of


organisms .

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biology 1.1 What is biology?

2 Characteristics of organisms:
a Reproduce and grow
b Obtain food and respire
c Detect and respond to stimuli,
and move
d Excrete to maintain a relatively
constant internal environment
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1.2 How can we study biology?


A Scientific method
Scientists study the natural world
using the scientific method.

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A Scientific method

“There is NO single scientific


method, but it usually involves
a few basic steps.”

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A Scientific method
Basic steps:
1 Making 2 Asking a 3 Proposing
observations question a hypothesis

Hypothesis is supported

4 Making a 5 Testing the 6 Drawing a


prediction hypothesis conclusion

Proposing a revised
Hypothesis is falsified
or new hypothesis
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A Scientific method
1 Making observations

• Bats can fly and


avoid hitting any
objects in the dark.
• Bats have small
eyes and big ears.

Lazzaro Spallanzani (1970)


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A Scientific method
2 Asking a question

How can bats avoid


hitting any objects in
the dark?

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A Scientific method
3 Proposing a hypothesis

Bats rely on their


sense of hearing to
avoid hitting objects
in the dark.

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A Scientific method
4 Making a prediction

If bats lose the sense of


hearing when their ears
are blocked with wax,
then the bats will hit
objects when they fly.

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A Scientific method
5 Testing the hypothesis

The bats with the The bats without the


sense of hearing do sense of hearing hit
not hit the wires. the wires.

Experimental group Control group


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A Scientific method
5 Testing the hypothesis
Control set-up
identical to experimental
set-up except that the factor
under investigation (i.e. the
sense of hearing) is absent The bats without the
The bats with the
sense of hearing do sense of hearing hit
not hit the wires. the wires.

ensure the result of the


Experimental
experiment is due group
only to Control group
that factor 34
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A Scientific method
5 Testing the hypothesis
Independent Dependent Controlled
variable variable variables
Presence of the
sense of hearing

Factor under
investigation
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A Scientific method
5 Testing the hypothesis
Independent Dependent Controlled
variable variable variables
Presence of the Whether the
sense of hearing bats hit the
wires and ring
the bells
Factor that is measured
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A Scientific method Factors that are


kept the same
5 Testing the hypothesis
Independent Dependent Controlled
variable variable variables
Presence of the Whether the Size of the bats,
sense of hearing bats hit the darkness of the
wires and ring room, number
the bells of wires, etc.

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A Scientific method
5 Testing the hypothesis
Bats with Bats without
the sense of hearing the sense of hearing

do not hit the wires hit the wires and


ring the bells
Experimental group Control group
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A Scientific method
6 Drawing a conclusion
The results support my
hypothesis!

I conclude that bats rely


on their sense of
hearing to avoid hitting
objects in the dark.
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A Scientific method
6 Drawing a conclusion

Sometimes the hypothesis


is falsified (not supported
by the result) ≠ wrong
 propose a new or
revised hypothesis

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A Scientific method
6 Drawing a conclusion

Hypothesis will be
subject to change with
more new evidence found
(e.g. ultrasound)
 Theory

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B Performing a scientific
investigation
Does the
presence of rice
speed up the
ripening of
papayas?
Kelly
Kelly’s mother

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Task
Design and carry
out an investigation
to find out the
answer to Kelly’s
question.

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1 Designing a scientific investigation


Aim
What is the aim of this investigation?

To study the importance


of rice in speeding up the
ripening of papayas.
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1 Designing a scientific investigation


Problem to investigate

What is the problem to be investigated?

Does the presence of rice


speed up the ripening of
papayas?

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1 Designing a scientific investigation


Hypothesis
Propose a hypothesis to the problem.

The presence of rice


speeds up the ripening of
papayas.

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Principle behind the design


a How can the hypothesis be tested?
Compare the rates of ripening of two papayas.

unripe
papayas
two identical
rice containers

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Principle behind the design


b Predict the results based on the design
of the investigation.

If a papaya is kept in a container with rice,


the papaya will ripen more quickly
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Principle behind the design


c Identify the variables.
Independent Dependent Controlled
variable variable variables
Presence of Rate of Initial degree of
rice ripening of yellowing, size
each papaya of the papayas,
size of the
containers, etc.
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Principle behind the design


c Identify the variables.
Independent Dependent Controlled
variable variable variables
Presence of Rate of How to
Initial degree of
rice manipulate this?
ripening of yellowing, size
Put riceofinto
each papaya the papayas,
one ofsize
the of the
containers
containers, etc.
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Principle behind the design


c Identify the variables.
Independent Dependent Controlled
variable variable variables
How to
Presence of Rate of Initial degree of
measure this?
rice ripening of yellowing, size
By observing the
each papaya of the papayas,
degree of yellowing
size of the
of each papaya
containers,
every day etc.
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Principle behind the design


d Is it necessary to set up a control?
Why?
Yes.
To ensure that the
presence of rice is
the only variable that
affects the rates of
ripening of papayas.
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Principle behind the design


e What assumptions are made in this
investigation?
• The two papayas are identical.
• The difference in the volume
of air in the containers is
insignificant to the ripening of
papayas.
*assumption ≠ prediction 53
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Procedure
What is the procedure of this
investigation?

Is there any precaution to be taken?

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Procedure
1

rice
identical transparent
containers
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Procedure
cover
2

unripe papayas
Experimental set-up Control set-up
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Procedure
3

Leave for a week…

Experimental set-up Control set-up


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Procedure
3

Observe and record the degree of


yellowing of the papayas every day without
taking them out of the containers.
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2 Carrying out a scientific


investigation
Results
Degree of yellowing (arbitrary unit)
Container Day Day Day Day Day Day Day
1 2 3 4 5 6 7
with rice 0 1 2 2 3 4 4
without rice 0 1 2 2 3 5 5
# The greater the number, the higher the
degree of yellowing
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Conclusion
The presence of rice
does not speed up the
ripening of papayas.

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3 Writing a report of a scientific


investigation
Writing a report after the investigation
allows other people to:
• understand what we have found out
• repeat our investigation

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Writing a report of a scientific


investigation
Aim Results
Introduction Discussion
Materials and Conclusion
apparatus
Procedure
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Aim

To study the importance of rice in


speeding up the ripening of papayas.

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Introduction
1 Problem 2 Hypothesis and prediction 3 Principle

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Introduction
1 Problem 2 Hypothesis and prediction 3 Principle

Does the presence of rice speed up the


ripening of papayas?

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Introduction
1 Problem 2 Hypothesis and prediction 3 Principle

The presence of rice speeds up the ripening


of papayas.
The hypothesis explains the problem.
(It is not needed in certain scientific
investigations.)
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Introduction
1 Problem 2 Hypothesis and prediction 3 Principle

If a papaya is kept in a container with rice,


then the papaya will ripen more quickly.

The prediction is based on the design


of the experiment.
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Introduction
1 Problem 2 Hypothesis and prediction 3 Principle
Includes:
a the rationale of the design
b the independent variable and the
way of manipulating it
c the dependent variable and the way
of measuring it
d the controlled variable(s)
e the critical assumptions made
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a the rationale of the design


In this investigation, the rates of ripening
of two papayas are compared.
Two identical containers are prepared.
One container is half-filled with rice.
One papaya is kept in the container with
rice, and the other is kept in the
container without rice.
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b the independent variable and


the way of manipulating it
The independent variable is the
presence of rice.
It is manipulated by putting rice into
one of the containers.

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c the dependent variable and


the way of measuring it
The dependent variable is the rate of
ripening of each papaya.
It is measured by observing the degree
of yellowing of each papaya every day.

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d the controlled variable(s)


The controlled variables are the initial
degree of yellowing and size of the
papayas, size of the containers, etc.

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e the critical assumptions made


It is assumed that the two papayas
are identical.
The difference in the volume of air in
the containers is also assumed to be
insignificant to the ripening of papayas.

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Materials and apparatus

List all the materials and apparatus,


and state the quantity needed.

transparent container 2
unripe papaya 2
rice
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Procedure

Write each step in the correct order in


past passive voice.
Also explain all precautions taken.

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Procedure past passive voice


1 Two identical transparent containers
were prepared. One of the containers
was half-filled with rice.
2 An unripe papaya was put into each
container. Then the two containers
were covered.

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Procedure past passive voice


3 The two containers were left at room
temperature for a week. The degree of
yellowing of the papayas was observed
and recorded every day.
precautions
Observations were made without
taking the papayas out of the containers
to minimize disturbance to the papayas.
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Results

Should be presented in
appropriate form(s), e.g. tables,
graphs, labelled diagrams,
photographs.

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Results
Degree of yellowing (arbitrary unit)
Container Day Day Day Day Day Day Day
1 2 3 4 5 6 7
with rice 0 1 2 2 3 4 4
without rice 0 1 2 2 3 5 5
# The greater the number, the higher the degree of
yellowing

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Discussion
Includes the following (if applicable):
a Interpretation of the results
b Possible sources of error and how
they affect the accuracy of the results
c Any anomalous data and its
significance

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Discussion
Includes the following (if applicable):
d Validity of assumptions based on
the results
e Limitations of the design of the
investigation
f Suggestions for improvement and
further investigations
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a Interpretation of the results


(e.g. trend or pattern in data, relationship
between the independent and dependent
variables)
The papaya in the container without rice
(control) ripened slightly faster. (result)
This showed that the presence of rice
did not speed up the ripening of the
papaya. (interpretation)
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b Possible sources of error and how


they affect the accuracy of the results
The degree of yellowing was not
consistent in different parts of the two
papayas. (error 1)
The judgment of the degree of yellowing
was also subjective. (error 2)
These lowered the accuracy of the results.
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c Any anomalous data and its


significance
d Validity of assumptions based on
the results

(not applicable in this report)

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e Limitations of the design of the


investigation
The container in the experimental
set-up was half-filled with rice.
The difference in the volume of air in
the containers might have a significant
effect on the ripening of papayas.

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f Suggestions for improvement and


further investigations
Suggestion 1
To increase the reliability of the results,
more papayas should be used.
Averaged data should also be used for
analysis.

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f Suggestions for improvement and


further investigations
Suggestion 2
The degree of yellowing of papayas
should be observed by more than one
person to minimize subjectivity.

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f Suggestions for improvement and


further investigations
Suggestion 3
Cards showing different degree of yellow
should also be used as a reference.

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f Suggestions for improvement and


further investigations
Suggestion 4
An equal volume of plastic beads should
be put in the container in the control.

plastic
rice
beads

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f Suggestions for improvement and


further investigations
Suggestion 4
This ensures that the volume of air in the
two containers is the same.

plastic
rice
beads

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Conclusion
• Relate the conclusion to the
hypothesis.
• Do not introduce new information nor
make generalizations.

The presence of rice does not speed up


the ripening of papayas.
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1.1
Simulation
Design an investigation of the effect of 1.1

fresh pineapple on the setting of jelly


Why did the I think fresh pineapple contains
jelly not set? something that prevents the jelly
from setting.

Peter Mary
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1.1
Mary’s hypothesis:
The fresh pineapple contained something that
had prevented the jelly from setting.

jelly solution fresh


pineapple
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1.1
Task
Design and carry out an investigation to test
Mary’s hypothesis. Write a full report of
your investigation.

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1.3 Nature of science


Nature of science
• about what science is and how science
works
• various aspects of nature of science can
be illustrated in the history of the
development of biological knowledge

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Example

Do organisms arise from


non-living things?

Francesco Redi
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For thousands of years, people believed that


organisms could arise from non-living things
by spontaneous generation.

meat maggots

Maggots arise from meat.


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For thousands of years, people believed that


organisms could arise from non-living things
by spontaneous generation.
non-living organisms
meat maggots
thing

Organisms arise from non-living things.


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In 1668, Francesco Redi observed that


maggots appeared a few days after flies
moved around a piece of meat.

flies

maggots

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He hypothesized that maggots were


produced by flies.

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Science advances through


reasonable skepticism
Redi questioned a long-held belief,
spontaneous generation. This attitude
drove him to investigate and later
spontaneous generation was disproved.

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Based on his hypothesis, Redi predicted:

If flies were kept away from


meat, maggots would not
appear.

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Redi’s experiment

gauze

jars

meat
Experimental set-up Control set-up
(jar not covered) (jar covered)
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Doing science requires creativity


and imagination

Redi kept flies away from the meat


using a piece of gauze.

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A good scientific experiment


must include carefully designed
controls
Redi did a controlled experiment.

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(P.1-15) What are the independent, dependent


and controlled variables in Redi’s experiment?
Independent variable: Whether flies are
allowed to reach the meat
Dependent variable: Whether maggots appear
on the meat

Controlled variables: Size of the meat, size of


the jars, temperature

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A few days later…

maggots no
appeared maggots
appeared

Experimental set-up Control set-up


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A few days later…

The result supported


Redi’s hypothesis
maggots no
appeared maggots
appeared

Experimental set-up Control set-up


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A few days later…

But the belief about spontaneous


generation continued.
maggots no
The discovery
appeared of microorganisms
maggots
(from nowhere) also strengthened
appeared
the belief of spontaneous generation.
Experimental set-up Control set-up
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John Needham’s experiment (1745)

boiling stopper microorganisms


the broth the flask appeared

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John Needham’s experiment (1745)

Needham’s conclusion:
The microorganisms did arise
spontaneously.

stopper microorganisms
boiling
the flask appeared

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Lazzaro Spallanzani’s experiment


(1775)
• did not agree with Needham for the
spontaneous generation of
microorganisms
• suspected that the time for boiling the
broth was not long enough to kill all
the microorganisms
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Lazzaro Spallanzani’s experiment


(1775) after a few
days
Experimental
set-up
boiling for a no sealing microorganisms
longer time of flask appeared

after a few
days
Control
set-up
boiling for a seal no microorganisms
longer time the flask appeared
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Lazzaro Spallanzani’s experiment


(1775) after a few
days
Experimental
These results
set-up showed that
microorganisms
boiling for a did not appear
no sealing microorganisms
longer time of flask appeared
in boiled broth when theafter flask
a few
was sealed. days
Control
set-up
boiling for a seal no microorganisms
longer time the flask appeared
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Science is a process of ongoing


inquiries

After the work of Redi, biologists


continued to investigate whether
spontaneous generation happened.

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Scientists have to explore using


different techniques and
methods to find out a good
solution to a problem
Spallanzani modified Needham’s
experiment.

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Lazzaro Spallanzani’s experiment


(1775) after a few
days
However, many
Experimental people were not
set-up
convinced.
boiling for a no sealing microorganisms
longer time of flask appeared
The belief of spontaneous after
generation
a few
was not falsified until almostdays
a
Control
century later by Louis Pasteur.
set-up
(P.1-20) Reading
boiling for a seal tomicroorganisms
no learn
longer time the flask appeared
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The nature of science is about what


science is and how science works.

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Some aspects of the nature of science:


a Science advances through
reasonable skepticism.
b Doing science requires creativity
and imagination.
c A good scientific experiment must
include carefully designed controls.
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Some aspects of the nature of science:


d Science is a process of ongoing
inquiries.
e Scientists have to explore using
different techniques and methods to
find out a good solution to a problem.
f Scientists build on the work of other
scientists.
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1 Which characteristics of organisms does


Sophia have?
Sophia can detect and respond to
stimuli, and move.

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6 Introducing
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biology

2 Why do biologists not consider Sophia


an organism?

Sophia does not reproduce and grow,


obtain food and respire, etc.

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Biology
studied by

the scientific scientific


study of method
part of
organisms
nature of
science
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6 Introducing
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biology

organisms
characteristics

detect and respond to


reproduce stimuli, and move
and grow
excrete to maintain
obtain food a relatively constant
and respire internal environment
124

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