Fertilizers – Method for determination of total phosphorus

TCVN 8563 : 2010

I. Reagents
• Vanadomolybdate solution
• Dinitrophenol 0.1%
• Phosphate standard solution
II. Preparation of Test Solution

Treat 2 g test sample, when these acids are suitable.

• Group 1: Dissolve in 35 mL HNO3 + HCl (1HNO3 : 3HCL), and boil until organic matter is
destroyed
• Group 2: Dissolve in 30 mL H2SO4 + 0.5 mL HClO4 , and boil until organic matter is destroyed

Note:

• For high P2O5 content samples, dilute to 200 mL.

• For low P2O5 content samples, dilute to 100 mL

III. Determination
• Standard solution: Pipet, into 50 mL volumetric flasks, 5 mL aliquots of standard phosphate
solutions containing 0.0 and 20.0 mg P2O5/aliquot, respectively, and develop color by
Dinitrophenol and Vanadomolybdate solution. Adjust instrument to zero A for 0.0 mg standard,
and determine 0.0 to 20.0 mg standard at 420nm spectrophotometer

• Test solution: Pipet 5 mL test solution, into 50 mL volumetric flask. Develop color by
Dinitrophenol and Vanadomolybdate solution in same manner as for standard phosphate
solutions in preceding paragraph, with instrument adjusted to zero A for 0.0 mg standard. Read
P2O5 concentration from standard curve.

IV. Calculation

Calculate Phosphorus content

𝑎∗𝑉2∗𝑉∗100
%P=
1000∗𝑉1∗𝑚∗1000

With:

a: mg Phosphorus from standard curve (mg P/L)

m: Weight of sample (g)

V: Volume of sample after destroyed (mL)

V1: Volume of testing solution used (mL)

V2: Volume of testing flasks (50mL)

Calculate P2O5 content

%P2O5 = %P * 2.291
With:

2.291: P to P2O5 constant.