First Sessional Exam.

BP 605T:
Pharmaceutical Biotechnology
(B.Pharm VI A+B)

1. Which among the following chromatographic technique is most commonly used

for enzyme purification
(1 Point)

Ion-exchange

Hydrophobic interaction

Size extrusion

Size exclusion

2. Glucose biosensor is an example of __________________ type of biosensors.

(1 Point)

Optical

Thermal

Amperometric

Conduct metric

3. The first synthetic polymer used for enzyme immobilization is

(1 Point)

Nylon
 Polystyrene

Silk

Polyamide

4. _________________ biosensors are based on the principle of acoustics and are

also called as acoustic (sound waves) biosensors.
(1 Point)

Conduct metric

Calorimetric

Piezoelectric

Optical

5. The extra chromosomal, self replicating, circular DNA molecules present in

bacterial cells are known as
(1 Point)

Plasmid

Vector

All of the given options

rDNA

6. Which of the following is NOT a physical method of immobilization?

(1 Point)

None

Adsorption

Microencapsulation

Entrapment
 7. Which bacteria is used for the development of nodules in cereal crops?
(1 Point)

Urokinase

Rhizobium

Clostridium

Lipase

8. Biological recognition element(s) or bioreceptors used in biosensor is / are

(1 Point)

Antibodies

Microorganisms

All of the given options

Enzymes

9. Which of the following is a characteristic of electrochemical biosensor?

(1 Point)

Detects the movement of electrons between electrodes

Detects the change in light adsorption

Detects the number of photons emitted out

Detects the angle at which electrons are emitted

10. Cutting of DNA molecules into segments is done by

(1 Point)

Urokinase

Ligase

Isomerase
 Restriction endonucleases

11. The term ‘biotechnology’ was coined by __________ in __________year.

(1 Point)

Karl Ereky and 1919

Arthur Konberg and 1917

Arthur Konberg and 1912

Karl Ereky and 1905

12. Genetic engineering technique is used to produce human insulin from non-
human source. Identify the microorganism used to produce the same.
(1 Point)

Bacillus subtilis

Saccharomyces cerevisiae

Clostridium botulinum

Mycoplasma haemofelis

13. 1,4-α-D-glucan maltohydrolase is another name for

(1 Point)

γ- Amylase

ß- Amylase

α- Amylase

None

14. White biotechnology explains the applications related to which of the following
fields?
(1 Point)
 Industrial

Aquatic

Agricultural

Medical

15. Conversion of C3 plants into C4 plants to increase the photosynthetic rate can
be done through
(1 Point)

Protoplast fusion

Recombinant DNA technology

Both

None

16. Type of biosensors used to detect urinary tract infections are

(1 Point)

Thermometric

Amperometric

Piezoelectric

Luminescent

17. Production of human growth hormones is an example of which type of

biotechnology?
(1 Point)

Blue

Red

White

Green
18. Which of the following statement is INCORRECT for a biosensor?
(1 Point)

The linearity of the sensor should be high

Chemical interference must be minimized for obtaining the correct results

Value of electrode response per substrate concentration should be low

Response time should be low

19. Enzyme immobilization is a process of confining the enzyme molecules to a

(1 Point)

Solid support

Reaction mixture

Liquid support

Semi solid support

20. Who is known as the Father of Biosensors?

(1 Point)

Karl Ereky

Arthur Konberg

Watson and Crick

Leland C Clark

21. Optimum pH for α-Amylase is

(1 Point)

6.7–7.0
 4.0–5.0

5.5-6.5

22. The correct sequence of gene manipulation for rDNA is

P. Generation and selection of DNA fragments

Q. Introduction of the recombinant vectors into host cells
R. Expression of the gene to produce the desired product
S. Multiplication and selection of clones containing the recombinant molecules
T. Insertion of the selected DNA into a cloning vector
(1 Point)

P-Q-T-R-S

P-Q-R-S-T

P-T-Q-S-R

P-T-Q-R-S

23. Which of the following aspect is not included in Gene cloning strategies in
relation to recombinant DNA technology
(1 Point)

Insertion of DNA fragments into host cells

Generation of desired DNA fragments

Selection or screening of the recipient cells for the recombinant DNA molecules

Introduction of the vectors into host cells

24. The process used to create same genetic replica of another organism is known
as
(1 Point)

rDNA

Cloning

DNA finger printing

 Gene mapping

25. The most commonly employed cross linked polymer used for enzyme
entrapment is
(1 Point)

EC

Collodion

Polyarylamide

Polyacrylamide Gel

26. Which statement among the following is CORRECT?

(1 Point)

Both Proteases and Peptidases are endocellular

Proteases are extracellular and Peptidases are endocellular

Proteases are endocellular and Peptidases are extracellular

Both Proteases and Peptidases are extracellular

27. Enzyme catalyzed joining of DNA fragments is called as

(1 Point)

Gene splicing

Gene mapping

Gene cloning

Gene sequencing

28. Using genetic technique in forensic science is also called as

(1 Point)

Gene Therapy
 In-vivo Culture

Genetic Finger Printing

Hybridoma Technology

29. ______________ is an immobilized enzyme is used for the production of High

fructose corn syrup.
(1 Point)

Glucose isomerase

Aspartase

Glucose hydrolygase

Nitrile hydratase

30. Gene transfer can be achieved by which of the following method(s)

(1 Point)

Particle bombardment

Plasmid

micro-injection

All of the given options

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