ANALYTICAL METHODS IN DRUG DESIGN AND TECHNOLOGY - LABORATORY

(Medicinal Chemistry, Ist semester)

……………………………………… …….….……………………
name, surname, student’s number date, time

……………………………………… …….……………………….
name, surname, student’s number group number

LABORATORY REPORT
High Performance Liquid Chromatography

Purpose of the experiment:

I. Isolation procedure
(describe very precisely every step of isolation of the main components of the drug)
II. Parameters of the Separation Method
(describe very precisely every parameters of the HPLC separation process of Gripex components)

1. Composition of the analyzed mixture

percentage concentration concentration of

mass of sample mass of each
component of each component each component
[mg] component [mg]
[%] [mg/ml]

Paracetamol 89.04 325

Pseudoephedrine
365 8.22 30
hydrochloride
Dextromethorphan
2.74 10
hydrobromide

Volume of the prepared sample: ……..

Volume of the injected sample: ……..

2. HPLC elution program

Components of eluent A: ……………………………………………………………………....

Components of eluent B: ……………………………………………………………………….

Speed of flow of mobile phase: ……………..

Program of gradient elution:

percentage of eluent B
Time of analysis
in the mobile phase
[min]
[%]

3. HPLC procedure

Temperature of the experiment: ……………………

Type of the detector: ……………………………….

Wavelength: ………………………………………...

Column parameters: …………………………………………………………………………….

(producer, size of column, type of resin, size particles, size of pores in particles)
III. Results of the experiment

1. Chromatogram
(paste here the received chromatogram)

2. Results and conclusions

(fill the table with results of analysis, describe them, put some conclusions and comment the precision of analysis)

Retention Percentage Percentage

Name of the High of peak Area of the
time [%] [%]
components: [mV] peak
[min] (experimental) (theoretical)

Component 1 4.134 3.698 154,411.684 0.235%

5.837 868.762 65,320,392.6
Component 2 99.523%
61
Component 3 30.438 0.75 158,972.725 0.242%