3/8/2020 PhysioEx Exercise 8 Activity 2

PhysioEx Lab Report

Exercise 8: Chemical and Physical Processes of Digestion
Activity 2: Exploring Amylase Substrate Specificity
Name: amilasa
Date: 3 August 2020
Session ID: session-d23d6cb6-f406-0650-d322-d04f13363a55

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1 Which of the following is true of enzymes and substrates?

You correctly answered: Enzymes are specific about the substrates they can act upon.

2 Which of the following is/are reducing sugars?

You correctly answered: both glucose and maltose .

3 Cellulose and starch are both

You correctly answered: polymers of glucose and polysaccharides.

4 Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide
bond. Which of the following enzymes do you think would be specific for a peptide bond?

You correctly answered: peptidase.

5 The Benedict's assay tests for the presence of

You correctly answered: reducing sugar.

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3/8/2020 PhysioEx Exercise 8 Activity 2

Experiment Results
Predict Question
1 Predict Question: Do you think test tube 3 (amylase, cellulose, pH 7.0 buffer) will show a positive Benedict's test?

Your answer: no.

Stop & Think Questions

1 You included tube 2 (amylase, glucose, pH 7.0 buffer) to

You correctly answered: see what a positive Benedict's test should look like.

2 Which tube is included to detect contaminating amylase in the buffer or in cellulose?

Your answer: tube 1 (amylase, starch, pH 7.0 buffer).

Correct answer: None of the above.

3 What is the usual substrate for the pancreatic enzyme peptidase?

You correctly answered: peptides.

Experiment Data
Tube No. Reagent 1 Reagent 2 Reagent 3 Time (min) Temp (°C) IKI Benedict's
1 Amylase Starch pH 7.0 Buffer 60 37 - ++
2 Amylase Glucose pH 7.0 Buffer 60 37 - ++
3 Amylase Cellulose pH 7.0 Buffer 60 37 - -
Deionized
4 Cellulose pH 7.0 Buffer 60 37 - -
Water
5 Peptidase Starch pH 7.0 Buffer 60 37 + -
6 Bacteria Cellulose pH 7.0 Buffer 60 37 - ++

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3/8/2020 PhysioEx Exercise 8 Activity 2

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 The substrate(s) for amylase is/are

You correctly answered: starch.

2 The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that

You correctly answered: peptidase does not digest starch.

3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose?

You correctly answered: The bacterial suspension contained the enzyme cellulase, which digested the cellulose.

4 Which tube allows you to conclude that the buffer was not contaminated with amylase?

You correctly answered: tube 5 (peptidase, starch, pH 7.0 buffer).

Review Sheet Results

1 Describe why the results in tube 1 (amylase, starch, pH 7.0 buffer) and tube 2 (amylase, glucose, pH 7.0 buffer) are
the same.

You did not answer this question.

2 Describe the result in tube 3 (amylase, cellulose, pH 7.0 buffer). How well did the results compare with your
prediction?

You did not answer this question.

3 Describe the usual substrate for peptidase.

You did not answer this question.

4 Explain how bacteria can aid in digestion.

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3/8/2020 PhysioEx Exercise 8 Activity 2

You did not answer this question.

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