Tuj-1: SGN

Primary: https://www.biolegend.com/en-us/products/purified-anti-tubulin-beta-3-
tubb3-antibody-11580?GroupID=GROUP686
Secondary: anti-Mouse (green)

myosinVIIa: hair cells

Primary: https://www.scbt.com/p/myosin-viia-antibody-c-5
Secondary: anti-Mouse (green)
Sox2: supporting cells
Primary: https://www.abcam.com/sox2-antibody-epr3131-ab92494.html
Secondary: anti-Rabbit (red)

Double label F4/80 + isolectin GS-IB4 for PVM/Ms and endothelial cells
F4/80 or Iba1: PVMs, myeloid cells/macrophages; myeloid cells;
Primary: KELLY HAS rat anti-mouse F4/80!!! Although it seems we need rabbit?
Secondary: anti-Rat (green), https://www.thermofisher.com/antibody/product/Goat-
anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006
Isolectin GS-IB4 conjug. to Alexa Fluor 568: endothelial cells ?
https://www.thermofisher.com/order/catalog/product/I21412?SID=srch-srp-I21412#/
I21412?SID=srch-srp-I21412? (red)

CD64: resident macrophages

Primary: https://www.thermofisher.com/antibody/product/CD64-Antibody-clone-1-
Recombinant-Monoclonal/MA5-29704?imageId=371336
Secondary: anti-Rabbit? (red)?

CCR2: infiltrating macrophages

Primary: https://www.abcam.com/ccr2-antibody-epr20844-15-ab273050.html?
productWallTab=Abreviews
Secondary: anti-Rabbit (red)

Fibrocyte ab – ask Danny, status of DMPO>???? nothing

ORDER ALL THESE ANTIBODIES – CC KELLY AND CHRIS!

Primary
Antigen Species Clone Comp Link
any
Tuj-1 (SGN) Mouse IgG2a Bioleg https://www.biolegend.com/en-us/products/
end purified-anti-tubulin-beta-3-tubb3-antibody-
 11580?GroupID=GROUP686
myosinVIIa Mouse IgG2a Santa https://www.scbt.com/p/myosin-viia-antibody-
(hair cells) Cruz c-5
Sox2 Rabbit EPR31 Abca https://www.abcam.com/sox2-antibody-
(supporting 31 m epr3131-ab92494.html
cells)
F4/80 Rat CI:A3- Invitro https://www.thermofisher.com/antibody/
(macrophages/ 1 gen product/F4-80-Antibody-/MA5-16624
myeloid cells) *KELLY’S LAB HAS*
Isolectin GS- Griffoni Invitro https://www.thermofisher.com/order/
IB4 conjug. to a gen catalog/product/I21412?SID=srch-srp-I21412#/
Alexa Fluor simplici I21412?SID=srch-srp-I21412
568: folia
endothelial
cells
CCR2 Rabbit EPR20 Abca https://www.abcam.com/ccr2-antibody-
(infiltrating 844-15 m epr20844-15-ab273050.html?
macrophages) productWallTab=Abreviews

Secondary
Antibody Host Targe Compan Link
t y
spec.
Donkey Donke Anti- Invitroge https://www.thermofisher.com/antibody/product/
anti-Rat y Rat n Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-
IgG (H+L) Secondary-Antibody-Polyclonal/A-21208
Highly
Cross-
Adsorbe
d
Secondar
y
Antibody
, Alexa
Fluor
488
Goat Goat Anti- Invitroge https://www.thermofisher.com/antibody/product/
anti- Mous n Goat-anti-Mouse-IgG-H-L-Cross-Adsorbed-
Mouse e Secondary-Antibody-Polyclonal/A-11001
IgG (H+L)
Cross-
Adsorbe
d
Secondar
y
Antibody
, Alexa
Fluor
488
Donkey Donke Anti- Invitroge https://www.thermofisher.com/antibody/product/
anti- y Rabbi n Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-
Rabbit t Secondary-Antibody-Polyclonal/A-21207
IgG (H+L)
Highly
Cross-
Adsorbe
d
Secondar
y
Antibody
, Alexa
Fluor
594
Donkey Donke Anti- Invitroge https://www.thermofisher.com/antibody/product/
anti-Rat y Rat n Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-
IgG (H+L) Secondary-Antibody-Polyclonal/A-21209
Highly
Cross-
Adsorbe
d
Secondar
y
Antibody
, Alexa
Fluor
594
Add anti-Rat red

Mike klein imaging core – ask about 3 colors

Repeat PCR and scope test on CMV-Cre

Write paragraph on methods on gender and laterality

Email anna holley to help with nbhs/ome study

Transplacntal mouse cmv infection

Infect pregnant mouse with ip cmv
Do hearing tests on pups