Name: Block:

Midterm Laboratory Exam

How do you carry a microscope? The proper way to carry a microscope is to grasp
the arm of the microscope with your dominant hand, lift the microscope up slowly, and
use your other hand to firmly hold the base to stabilize the microscope as you transport
it from one place to another

1. If the ocular lens is 10X, what is the Total Magnification of each objective lens?
a. Scanning (4X):: 40X
b. Low (10X) :100X
c. High (40X): 400X

Which knob should you NOT use on the high power? WHY? The coarse focus
knob because if you turn the coarse adjustment knob while on high power, the
objective could easily break your slide.

2. What is the magnification of the eyepiece? The common ocular magnifies ten
times, marked as 10x
3. Why is there an X after each power, like 4X or 100X? Because the X represents
the magnification.

4. What is the name of the lens in the eyepiece? Ocular lens

5. How do you prevent a slide from falling off the stage? Use the stage clips

6. Which adjustment knob is used for first focusing? The coarse adjustment knob

7. Which adjustment knob is used for sharpening? Fine adjustment knob

8. What is the function of the diaphragm? Controls the amount of light reaching the
specimen

9. How do you change the objective lenses? Turn the revolving turret so that the
lowest power objective lens (4x) is clicked into position. Place the microscope
slide on the stage and fasten it with the stage clips. Look at the objective lens
and the stage from the side and turn the focus knob so the stage moves upward.

10. What power must be in place when you first place a slide on the
microscopestage? 4x
Name: Block:
11. Why is it called a compound light microscope? Because it is a tool
containing two lenses, which magnify, and a variety of knobs used to move and
focus the specimen

12. Increasing magnification decreases field of view. Therefore, which

magnification power will have the smallest field of view? D. 400X

A. 10X B. 40X C. 100X D. 400X