MICROSCOPE

• Prepared by:
Ms. Marisol C. Marcelo, LPT
 Microscope

 An instrument used to see

objects that are too small
to be seen by the naked
eye.
 Rules of using a microscope
Always carry with 2 hands
Never touch the lenses with
your fingers.
Only use lens paper for cleaning
After using, rotate the
nosepiece to low power
objective, roll the stage down
to lowest level and rubber
band the cord,
 Parts of the
Microscope and
Their Function
Parts of a microscope

Eyepiece
Where you look
through to see
the image of
your specimen.
Contains the
10X
magnifying
lens
Parts of a microscope
Coarse
Adjustment
Knob
Moves stage up
and
down
Focuses the
image under
low power
Use this first
Parts of a microscope
Fine
Adjustment
Knob
Focuses the
image under
high power
To fine-tune
the focus of
your specimen
after using
the coarse
adjustment
knob.
Parts of a microscope

Revolving
Nosepiece
Holds the
objective lenses
Revolves to
change
magnification
Parts of a microscope

Objectives
Low power
(shortest)
Magnifies 4X
Medium power
Magnifies 10X
High power
(longest)
Magnifies 40X
Parts of a microscope

Stage
Supports and
holds the
microscope
slide while
viewing
Parts of a microscope

Stage clips
Place on
microscope slide
to secure into
place after you
have focused on
your specimen
Parts of a microscope
Diaphragm
Controls
the
amount of
light
available
while
viewing
sample
Parts of a microscope

Light source
Shines light up
through the
diaphragm and
stage to light
the object
being viewed
Lighted area is
called your “Field
of View”
Parts of a microscope

Body tube
connects
the
eyepiece to
the
objective
lenses
Parts of a microscope

Arm
Supports the
body tube
which
connects the
eyepiece to
the set of
objective
lenses
Parts of a microscope

Base
Supports the
microscope
 Total
Magnification
Multiply the eyepiece
magnification (10X) by the
objective magnification (4X,
10X, 40X)
Example: 4 x 10 = 40X total
 How to use a microscope
•Place the slide on the stage
•Use stage clips to secure slide
•Adjust nosepiece to lowest setting
• (Lowest = shortest objective)
•Look into eyepiece
•Use coarse adjustment knob
•Use fine adjustment knob