SEMINAR NASIONAL

BALIKPAPAN, 16 MARET 2022

Biosementasi
Stabilisasi
Berkelanjutan
Dr. Ir. Andi Marini Indriani, ST., MT
Tanah Lunak Masalah
Klasik Dunia
Konstruksi Jalan
DAYA DUKUNG RENDAH
PEMANPATAN TINGGI

Persebaran tanah lunak di Indonesia cukup merata,

melihat luasnya daerah tanah lempung yang dimiliki
hampir di setiap pulau.
BIOSEMENTATION
EFFECT Microbial-induced calcite precipitation
(MICP) is a process that uses naturally
occurring bacteria to bind soil particles
together through calcium carbonate (CaCO3)
precipitation

It is a promising new technology in the area

of Civil Engineering with the potential to

become a cost-effective, environmentally
friendly, and sustainable solution to many
problems such as ground improvement,
liquefaction remediation, enhancing
properties of concrete, and so forth.
 PROCESS OF CHANGING SOIL
INTO
"CEMENTED SOIL"

(The sand is inj ected with (The sand is inj ected with
bacteria once) bacteria twice)

(The sand is inj ected with bacteria (The sand is inj ected with
four times) bacteria seven times)
 BACTERIA
SOLUTION

growth mediA

The most commonly used urease

bacteria in past studies are Bacillus
subtilis, S. pasteurii, Spoloactobacilus,
Clostridium, and Desulfotomaculum.

bacterium with highly active urease bacterial isolate

enzymes
bacillus subtilis culture 3
days
 bacteria breeding

Bacillus subtilis culture

culture was carried out on B4 medium
with the following composition:
Urea 20 gr,
Nutrient Broth 3 gr,
NaHCO3 2.12 grams,
CaCl2.2H2O 4.14 gr
NH4Cl 10 grams.

Bacterial Growth Test

To determine the growth of bacteria,

turbidimetric testing was carried out.
Turbidimetric testing is testing the number
of bacterial cells contained in a solution by
measuring the level of turbidity of the
solution expressed in Optical Density (OD).
 CALCI TE DEPOSI TI ON ON THE
SURFACE OF THE CBR SAMPLE
 CALCI TE DEPOSI TI ON ON THE
SURFACE OF THE CBR SAMPLE
MI CROBI AL-I NDUCED CALCI TE PRECI PI TATI ON (MI CP)

BE FORE

AFT E R
U N C O N F I N E D C O M P R E S S I V E S T R E N G TH ( U C S )

 THE EFFECTIVENESS OF
MICP IN CEMENT ING SOIL

biogeochemical process chosen

type of bacteria

concentration

pH and temperature

concentration and volume of cementation solution

 CURI NG EFFECT
Biocementation is the continuous
improvement of soil as long as soil
nutrients are still available
The ripening period has an important role
in increasing the strength of the soil.
The longer the curing period the increase
will continue to occur
Translation results
Soesanto (2008) Bacillus subtilis
bacteria can survive in nutrient-poor
soils by turning into endospores and
can survive for 6 years and can divide
again.
if nutrients are available again this
means precipitation of CaCO3
can continue to occur and the increase
in soil strength will continue as long as
the nutrients needed by bacteria are
still available in the soil.
According to Mujah (2019) the addition
of nutrients to the soil can again
increase the availability of bacteria in
the soil
small scale model test
DCP test
correlation
of
dcp And cbr

soil clasification by wesley

 EFFECT OF TEMPERATURE ON
MECHANI CAL BEHAVI OR

Research by Ng et al
(2012) regarding the subsurface
temperature, it was found that the
urease activity leading to the rate of
CaCO3 crystals increased with
increasing temperature
Calcite
deposition in
the test bath
application the soil is sprayed with a
bacterial solution then stirred
and compacted

Terima Kasih!
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