Nucleic acid probes Natit wtic yosuetseb wou Wbed fo witut pep daquene § Tore pna_dt_ RNA roplenleb yours a meer Th wong Abzemcded Aku sulin coliel pocebe chupboiclin® ato pu eLeraplammesabosey Longet DNA Bt RNA dat us drum popes un duasgnenis uy penitn ibie bes Nucleiz vorticl duplsricligatien Js opis. yt g wuliall btu vemplarasibany pievatrede Depiniten ie —~—Sn BNA techootegy cidletleotlag Birgu otnamded rule atid Moleruk Uv 16 wooed tO 14 a Upeipe Lerydem ~ rriaky ; win oy ! d nucleoli Bequaert Tuli woud Sample4 Types of nuclele acid psobes = 1; Genomic DNA probes i- using prueires, TB vip, ua i spengmu ts, _ _ gear 3atu Obey purupied cand lalseLledl a CONA poobes ; later SA Prom waning RTase presen cand dabeliay 3. syommenne oli al gopucleande probes, Maca cla y t= 80 bare Ut: NA poobes i~ BAe OTe 1 ecarts RNA moby, Cdn un vitseo eee op DNA el 4. DNA ppoveles vasa Vou Spelfi un Duis Jupoicligation eusith patti dorrplemumtoriy DNA — eget DNA prebe TLS qpoceolul Yyolar sve 6 potee - ve DAG ypoLBbes core mgu stave wa vubintamn t tho diwodt dna! Dus tole they lam Use cubed 1(prorat re DNA tern Coad ASScus The S538 a wu ONnA probes CUNO woe 13-100 6P uot mu dhetp rao per. DNA Deguemted clam be carafe’ up to rullien golole Labels of RNA pyabes i- Labelled woth oreckenuthe, - pap. 129, 635, NB asin) fhasiatunt Lolsels - im, GUrecarrvims, EtNId!UM Us int, TLR , — ealRotuu yates ; PBL Stalinh yaargeeclodt Metneds empleye? 10 poobes '~ BR aruat mapeily one tome vos uhwriivelly bsntnns ue yolsdiatey. thot vase, drecsesen MAG inet waif ws upetaimimg ere - isolotion gy ulited vupioms ge celening obtain ONA«4 umtact ugemas gpoeimg SO Tgolation of selected SEgion QF Genes , The DNA ypwm van ais, (aay yratnegen) an de hut aang vastrsdon andonutias 4, DNA wegen wou ered um y SC ward de DNA prBbes wan be Selec, by abuent Synthesis OP » ows peobes fsom maya, The MRNA meleelis Speeyic elo co qrortitulan DNA Pagers Cerca a poten) vara Isolated - % ong “he e } UWMSSLOL DNA CCOND) TBL ae Laue pmiged | this CDNA, wam kk pee to volutes tte forges oe iprcobe Z Dna pee assays! ~ th paints 4 Steps goken, yopootatien te doch SURE ipeepeeeea qh. warplen ike blood, FF ure, cxtool gt FiSSUsd, OSL cLotleclee yr potrogunuc Begamirse * wilesAuporution x du deere DONA rmblecuh: CUA pours walbdtot, tite Penruieise, 4 mutt muuranital & Mwrritoy SLOTS - Th. Foxgee metuLes may be dmuuased bY PCR ai LER: ete--- Hyboidigation:- DNA proter Dre caclded to PNA ccltited ptr thu Samp! camd voLLevsirtg cLerryplarcurrte rte DNA to joins ut may de hasutied cout &17hen wed chutnogunisus Bt Nemegurucus YOSVEULDD . ; : i Het Db ULDab bli. _Buppdet co Imumelsiligs Tonget DNA: Exormyples corn dst blot & om Situ puplricliy ation Hor eg Ps ots um wmelutien-vamd apt i ‘Gr, coset DNA uD» sa eecerr | immobil, ont Coens Lika fob “et ead dhernegunesus prceurs wolleuss vtapict sam me uppumk unyloucigoton Separation :- Ueluuren ubserrateyepNy ’ wluconepnéusis wt spaxcmagetat Pat | Moy us undud 1 vtecover Tous hybrict rb ELLIS Lq jection techniques i- i Dees viadio. dnotepuc, Gee2, pusunt Bt preteens MED Ley techniqn m4 be oe LAU SP Pron, utackto iso a cusith Py odes cai eas p32, 335, H3) ct Sreonrteme oy, b conn é Taf pe Oe ca a NA del sive eae oT oY © Fl 8 Loses, Tabet pNA Cc tag cen Retains fluosesceng DNA psdbe labeled with flvosescent dyeapplications of One pZebse! Genetic engincesing 56 DNA powbe UCUtL wu ulpnes porrerhing SPcugic d 1 lderhfy toamy- ened ic eanch i~ Uured te idemtiby DNA ueqpenrted vam fSumrao prosachoing apecygie g eUrostanlow The ypecalee> cam be vsed jn won wtucliee um motrartan biology papulotion gurutis & eceteg scot gence pease Diagnesie/ Epidemiolog ical shares NA ele wore urbed to iden rity cpemutic Bt_munabiot wlinwabed et wWraniges in ubhe DNA Ua ustaen temnswe Tdentigication of food contaminats Tt us udsd 10 j olernitify patino- d Vistuds un mpctied goed Samap > Fosensic investigations’ stses cages ured © ddlerrty remiss, wpetusing _voliepuFect poteunity wabss. urtabiahing garnily wuletens §€ wetwing inion pucleen Cand pggiculusat applications i- Ase tse +o idenhfy goed Vanicher & seedd dt plans. TOSONOMY A sabes yas uLbed to identify ‘ Bt Shecaun lext. um vangthe ppotagicel € arile- Nadas discaner t anions pal pele ONA poses wos urbe Suousey Uuurerun 0talLed dhiapneais uf yynhvatdCligonucleatide pyobes 4 thes OIn apn aged | wp blige wtolcdeb Uzded cen thy |, rratuodl ie potted en pu 2% ve ag ure preliin ug intenert The» eligo ruthacsticled Lay Wy OL W. Ulu be é Uh gue ees yor TNE postr, i poudelsn House. volo to col uu cpunutie sects toraknsctiod 4 ots netic Lossuid but stot thet warn O47 un mutienim tip pry upd Gt usin Ely @ _CtEN>- verre) Binerulsstich poets dd uded 0 Soup cof lbsoxies - it SS eee, Applications i- ums CDNA & epson DNa bh idertifying ot duledting SpeLific eu Eocasmining gemerit DNA & Per DNA vaseyp fied BIST eraeprUbbxOn libsomes idemheicatian % alern tok oy Dppie JADE change. BNO peeDe aie 6 vase Aabutiub & RNA dnp ~ tinorndect RNA ube 40 alites! » pers Lora lemnunkorty nmecduguurees Ctonge weguonirr > by hylan clizatren RNA an Loe Adu ally tahely, 0 ple Udi vLaclsi Soop ©, epitopes ipistin ust prropndier do medble Theos Rava prebes var chydnciizah on feels suracuun popula secetine q several cacluatogee canrecoated usith tna Se TET as gocebe Laser aye pe Dy in ike fowama yp tion peor Lulsedituted Her DAA preee in neestly voll capplitalipns Hien fi cous ty RNA Bt ies m volo O& ayrteniged yom pNnA tate, Clorié Len vette bei on SpSs in TL systems RNA aa postu = Bingt~ slond. wore advantagis Overt wae et inpotoved Sap él 2) midi ds Gulab uy alse noluon uy RN qo RNose adatten 1aNe prweom mud be aa ith the sarm Cory 8 any Othen RNA pour aah one tha inkoinaically a & the SuncephoitTn vitoto fotame Lr prlrey, 4 Loree mutivag § RNA probed h Lange carneunt> Yhibieng y, : : lalseled pro gy wunigsum dengan 1 0 ( ue guovrated vy Pama Goy Bag pur lig od newt to an RN» tr ¢ promote a vulabk €& uno return ¢ uU Poe panat ion _oF RNA psobes i- OURO RNA proce vrei any TJ joti sem 1 ened, yxipten ¥ wo. Hargt gum sed! 0. * plabsrnicl Toramabuplier oy The is di tined yum of poor ‘nel opprong ouegrined doy an RNA prolyprrwar Ous to aurrenpenpty pol suerte , pepe Pie oP fer the prrcometn wy 08 elsartord- prage ee | Up git gor Ae phagt prolyrurvens - DOCU SURLOTS WI fcuk witiged phage C5p6) TF povorneter én srichestg usnpigrs 10 jotamacw Hi % RNa1 a DN Ctongel gem) lend IN @ plarmiol year: i yet uw heey I The u Le inapadannd Pon ined pag premem Woe cbeth lord in’ wa veut & @ _ ue egusre yet tovaumavup hen inoert Aearatowarm g tu phage prcomoten uals cusuth otedam- Usumamt ONA cose wg whet eee prenract nuclectvt nutetich iS crackin lalselled - 7, ' wa in Inserted i Tabselled & may Ot Uned a> RNA porpber- gon ange’ Vere Appli cati ons i- Novinesn Blotting - RNA depsxotian sy Wletbusprsup' s ( aa : in Lermerhorey Detection Large hy douicliged prsled +Ansitu hybsidization - ; To Guical ch, uctatien GF PEO 1X Sloperry, con LIULErLEROTU in HSSuey. Bio medical - petectier &F aa Sea bev, e~ actnomyces, bactents of food spoilage. highly SEnsidve examp! petectter wotection assay - vomnd =Sequuns DIP LIGE RNA P