Professional Documents
Culture Documents
International Journals
By Raymond J. RITCHIE
(BScHons, PhD, University of Sydney)
7 December 2021
For the scholar who studies or works in a university, doing scholarly research and publishing the
findings in the form of articles in peer-reviewed International Journals is one of the most
important academic activities. No-one really takes you seriously until you have published a
single authored or at least a first-authored paper. I hope this publication is a very good
opportunity for learning how to publish papers in International Journals by someone who
actually does know the complete process of how to publish papers in International Journals.
Working in SE-Asia I know that >95% of global science is published in English but about 80%
of your readership is using English as a second language
Most of my papers are single authored and I am the first author (and Corresponding Author) on
most of his papers. I conceived the projects, did the experimental work, drew the figures,
prepared the tables and wrote the papers. I submitted the papers and have had to deal with the
journal editors and respond adequately to referee’s reports.
My papers are on a wide variety of topics on a large range of organisms: Bacteria, Fungi, Plants
and Animals and I am something of a polymath. I have also published papers in Science
Education, Theoretical Biology and Social Science Research and Regional Geography. Here the
emphasis will be on practical information on how to do it and what I have learnt from
experience. What works what does not? As a published scientist I am regularly sent papers to
referee by International Journals.
The aim is to teach students and staff how to independently publish papers in international
journals rather than simply writing in English. A good guidebook is Day (1998). It is on-line:
newer editions you have to buy. Things have not changed much except that nearly everything
today is now on-line.
The complete procedure of getting a paper published will be gone through step by step. Most of
the content is about conceiving the paper, getting organized, writing the different parts of the
paper, how to use references and choosing the most suitable journal.
The problem of nefarious journals will be discussed including the use of Beal’s List and the
Thomson-Reuters ISI rating to identify journals which you should not submit papers to.
Getting started:
How to Publish 2021Version - 5
• The Science is pointless unless it is published and published in the right place. Do not
bury your work in obscure places.
• The first requirement to be able to successfully publish scientific papers is to have the
confidence to write one and submit it.
• Delicate sensitive types will never get anywhere in science. You need to have a thick
hide like an elephant.
• Important Questions:
Appropriate Journal
A Target Journal
When you start writing a paper it is a good idea to have a Target Journal in mind. If a journal
has published papers like yours before there is a good chance they will consider your paper.
Sending a paper to the wrong journal means discouraging and rude rejection letters from the
editor and wasted time. In this example the obvious target journal for this manuscript is Aquatic
Botany.
You have identified a potential target journal based on the frequency of citation of that
journal in your manuscript. The next consideration is the status of the journal.
• Never send a paper to a Beall’s List journal even if papers from such a journal
are cited several times in your paper. You should as a matter of principle avoid citing
Beall’s List journals because it only renders respectability to them.
• The most frequently cited journal might not necessarily be the best choice for
your manuscript. You need to check the status of the journal. Use ISI (Institute of
Scientific Information) Rating as a guide. Who to you want to read your paper?
• Do not take ISI too seriously, just check that the journal has one. That is the critical
issue, not the actual number rating.
• For Example: In scientific research on photosynthesis there are two key journals. You try
one journal and then the other.
• Photosynthesis Research – ISI 3.502 – Springer-Verlag
• Photosynthetica – ISI 1.409 – Springer-Verlag
• I have published in both journals. They publish the same sort of papers. Same
publisher. Your papers are sent to the same referee’s so why the big difference in
ranking?
• Photosynthesis Research is run by the Germans.
• Photosynthetica is run by the Czechs.
• The person that did most of the work is always first author. Never allow any argument
on this point. The best way to make this clear is to write the title page with you as first
author. If you did the work you are in the driver’s seat. Everyone else is a passenger
including your supervisor.
• Never give your work to someone else to write up. Writing up is part of the job. It is
irresponsible and causes trouble.
• My experience has been that if possible it is best to write a paper by yourself or with a
single co-author.
• The paper with the student or post-doc as the first author (the junior scientist) with their
lord and master (their supervisor) as last author is a standard model that dates back
centuries. It is still common today because it works. For example, my fist paper was
Ritchie (1982) but numbers 2 and 3 were Ritchie and Larkum (1982a, b). I am still
publishing papers with my old PhD supervisor (Ritchie and Larkum 2012) and Ritchie
and Larkum 2016), Ritchie, Larkum and Ribas (2017), Larkum, Ritchie and Raven
(2018).
• Try and get a definite agreement on the number of authors.
Authorship Problems
• Once you start writing a paper people start taking you seriously. That has good and
bad points. You will find that most people are very encouraging. On the downside it is
a little bit like inheriting a fortune from a rich uncle. Suddenly you find you have more
friends than you thought you had. Hence, you need to be careful. Your paper is
valuable material. It can be stolen.
• Do not put somebody’s name on the paper just because you think it might help to get it
published. This is not how the world works. Nominal authorship is a very bad idea.
Only put people down as authors if they actually have made a contribution to the
paper. It is difficult to remove authors.
• Make sure people do contribute. If you find that one of your co-authors does not do
anything useful it is very hard to get their names off a paper. They may do nothing but
• Nothing I have ever done has had any commercial value and so I have never been in
difficulties about patents and intellectual property (IP) rights but have heard a lot of
horror stories from colleagues.
• Once you publish something in an International journal it becomes public knowledge
and so generally cannot be patented. Patent first. I have one manuscript with a colleague
which featured a potential patentable device. I advised her to get the patent first.
• IP rights can prevent you from publishing your work. Ask for legal advice from the
Research Office. For example, if you sign a contract with a prawn farm company to work
on improving their prawn food they can stop you from publishing it. The confidential
report you spent months writing for them does nothing for your career. Even years later
they may still not release it for publication.
• As I have said I have ever been involved in writing a paper where there were questions
over commercialisation and intellectual property rights. In the above case I mentioned I
advised the first author to patent her device before attempting publication. She now has
the patent. We are now trying to publish the work.
• I personally have never had to resort to an authorship agreement in writing but in a few of
my papers I wish I had insisted on some written agreement.
• In the case of most scientific papers authorship questions can be settled by having a
meeting of the authors of a paper and questions resolved by mutual agreement. However,
if the first author is young and inexperienced they may need advice and help from a
trusted senior co-author – usually their supervisor.
• If authorship is a complex issue and could lead to trouble. You might need a signed
agreement. But legalism can backfire and generate ill feeling.
• You must have a clear statement of what is the problem your paper is addressing and
why it is important.
• A clear outline of what the paper is about is needed which shows that that you
understand the problem. You did not do the project simply because you were told to.
• Make the aims of the paper clear in the last paragraph. You can even put in a single
sentence about what your paper has shown.
• The Introduction is not intended to be an exhaustive review of the subject but it needs to
be fully referenced to demonstrate that you are familiar with the literature. This is
important.
• Use scientific journals and books as your sources, references and authorities (Primary
Literature). Avoid other literature. Even published conference papers are often
difficult of access (no pdf) and so are generally are not regarded as primary
literature.
• You do not bring up issues in the Introduction that you do not deal with in the paper. It
makes your paper look incomplete.
• If your own work does not shed light on an aspect of a topic do not bring it up. When
you have finished the Discussion you need to re-evaluate the Introduction. Topics
brought up in the Introduction but not dealt with in the paper can be either removed or
added to the Discussion as future avenues of research. Talking about new avenues of
work in your Discussion improves the look of your paper.
• You may need to say what your paper is not about.
For example “Extrasolar planets have been found orbiting G, K and M stars but here we do not
discuss planets of K stars because so few are yet documented”.
• You are joining the big school now. Take yourself seriously.
The Introduction should demonstrate clear evidence that you critically read the literature and
identified the problem.
• “Critical thinking is not a set of skills that can be deployed at any time, in any context. It
is a type of thought that even 3-year-olds can engage in—and even trained scientists can
fail in.”
– Some brilliant scientists can be very credulous and remarkably easy to fool, especially if
they are told what they want to hear. See the chapter on scientific fraud.
• “Knowing that one should think critically is not the same as being able to do so. That
requires domain knowledge and practice.”
– Very true, you do need practice to understand science and you do need to learn to be
sceptical.
One of the more comical things about Science is that commentators on Science usually have no
scientific experience themselves and hopelessly confuse “Training” and “Education”. In general,
training in the sciences is necessary but is not sufficient to be a good scientist.
• “Teaching content alone is not likely to lead to proficiency in science, nor is engaging in
inquiry experiences devoid of meaningful science content.”
• “Subjects who started with more and better integrated knowledge planned more
informative experiments made better use of experimental outcomes.”
• The more general your level of science knowledge the better. Read news items in New
Scientist, Nature and Science regularly.
• Of course the Americans think you have to organise a course to teach critical thinking but
I doubt if it necessarily works. That is the training vs. education problem.
Submitting a Paper:
• When submitting a paper it is critical to write a proper Cover Letter such as the
example below. Remember that an Editor can reject your paper without sending it
out to referees. Be very polite. Usually trying to argue with an Editor is futile. Their
journal is their very own little empire and they do what they please.
Yours sincerely,
Dr Raymond J. Ritchie
M&M sections of papers are often badly written and provide inadequate information. Do
not contribute to this unfortunate modern habit. Remember your results need to be
reproducible.
Never write M & M like a cake recipe in present tense. Always use past tense, preferably 3rd
person. The cake recipe format makes you look amateurish and editors will reject your paper.
Non-English speakers often work very hard on improving the English of the Abstract,
Introduction and Discussion and neglect the English in the Materials and Methods and the
Results. They foolishly think that the Materials and Methods are “technical” and do not worry
about the English. If people cannot understand what you did and how you did it your paper is
unlikely to be accepted.
Materials and Methods are often inadequate in both old and modern papers but this
problem seems to be getting worse.
This is not just a problem in the hard sciences. Social science papers often have very poor
descriptions of what methods they used as well. If you use a reference to cover a method that
you used you must actually look at that paper to see if it really does actually gives a useable
description. Some references are used out of habit because everybody uses them. That is very
bad science. You might get a rude shock if you actually look at the actual paper.
If you used microbes, plants or animals for your study you must give an adequate taxonomic
description at least when you first mention them. You can get away with human, cat, dog, rabbit,
rat or mouse without the Latin names but you have to specify the strain used. For peas, beans,
wheat, rice etc you need to use the Latin names when first mentioning them and the strain you
Caution is needed with common names of plants. Some crop plants have different common
names in American-English and English-English. Look up what “corn” means in American-
English vs. English-English. Confusingly Australian English is often a hybrid, for example Zea
mays (Indian Corn) is universally called corn in Australia in the American sense and the English
habit of using “corn” for any cereal is not the way the word is used in Australia. Some oddities of
types of English: Americans habitually talk about time of year by American season, British &
Australians usually talk in terms of month. Archaic terms like “Fall” are not used in more
evolved forms of English and the term is never used in Australia because eucalypts are
evergreen.
Make sure you give an adequate description of both. For plants the minimum is temperature and
light conditions. Culture media recipes have to be carefully checked. The biological literature is
littered with inadequate descriptions of culture media. Essential materials such as culture media
should be either presented in detail or referred to an easily available source.
Sometimes the original description of the medium is unobtainable, in which cases you try and
find the oldest complete description that is in the open literature and use that as your source
material. Some culture recipes are frustrating: one I know of has 5 different versions!
Do not use a thesis or conference proceedings as sources for such information. Reason? Poor
accessibility and they are not Primary Literature.
Scientific Instruments
• Remember in the M & M you are trying to establish that you knew what you were doing.
Standard analysis texts can be very useful but references to big reference books need
page numbers e.g. (APHA-Standard Methods, 1998, p 1234).
• If you do anything involving human subjects or animals make sure you obey bioethics
procedures and say so in your M & M. A journal will not accept your paper if there is
any hint of bioethics problems. Sometimes the rules are quite strict. I have fish and a pet
turtle in the lab. I even have pet crayfish. When the ethics committee inspected my lab
they were quite happy with my pet crayfish, fish and turtle. I asked if I would have to get
ethics approval to do any science on them. I was assured I would have to get official
ethics approval.
• The M&Ms should provide enough information for it to be feasible to repeat the
experiments and is properly referenced. After all, that is what M&Ms is actually for!
• References used in M&M must be readily accessible; this is just as important in the
M&Ms as in the Introduction or Discussion. Avoid “grey” (Non-Primary) literature as
much as possible. If you have to use grey literature simply write it out in full and state
clearly where you got it. For example, give full details of the culture medium recipe, the
• The incomprehensible table has a great tradition in science. Try to avoid information
overload.
• Conclusions based on statistics need to explicitly stated and it must be clear what data was
used, the statistical tests that were used and the P values must be quoted.
• Try and keep tables as small as possible. About 50 numbers should be the maximum. It is
better to have 2 or 3 smaller tables than one big one. A big one is best put in an Appendix
or Supplementary Material.
• Explicit verbal description of results is required. Tables do not explain themselves.
• The clumsy incomprehensible table has a great tradition in science. Try to avoid
information overload.
• Conclusions based on statistics need to explicitly stated and it must be clear what data
was used, the statistical tests that were used and the P values must be quoted.
• Try and keep tables as small as possible. About 50 numbers should be the maximum. It
is better to have 2 or 3 smaller tables than one big one. A big one is best put in an
Appendix or in Supplementary Material.
Here is an example of a truly terrible Table (from a paper I co-authored) (Kaewsrikhaw et al.,
2015). This is an example of information overload. The latest craze is to mark significant
differences by superscript! Does not help! I find them difficult to grasp.
By the way, this is how the table is likely to appear in a publication. So small you cannot even
read it. Worse, if you make a mistake or the editor or the publisher try to change it is virtually
impossible to find the mistakes.
Napoleon lost nearly all his men, in particular those who had marched with him and had reached
Moscow. Of 350,000 men only 10,000 got out of Russia but it is worse than that. Most of the
survivors had been on garrison duty in places like Smolensk perhaps only a few thousand
Nice Figures with good presentation. But usually journals like to put in their own headings. Red
& Blue is OK for nearly all colour blind people but Red & Green is no good.
This is not the civilized way to write a discussion with your colleagues.
This is what can happen. Do not laugh. Do you seriously believe that this paper will ever get
finished? You must have a logical order of revision of your paper or you will never get it
finished. A simple linear model is most likely to be successful. This is the responsibility of the
first author or the corresponding author who might have more experience than you in dealing
with such nonsense.
A (First
Author)
E B
D C
This can end up as a hopeless mess. The paper might never get finished.
The Cycle Model (ABCDD then back to A) can go on forever and is limited by the slowest
and most dead-beat author. The first author can find themselves the author of something they
have lost control of. They can also find themselves the author of a five author paper when
they really only wanted to write the paper with one other person.
If Authors start squabbling amongst themselves you might never see the manuscript come
back.
Author B
Author C
My Comment:
An obvious problem with monster authorships is who actually did the work and wrote the paper?
This can be a critical issue in patents, intellectual property rights and scientific fraud
investigations. Sometimes Prof Bullfrog and Prof Dracula get their name on a paper they do not
want to acknowledge they ever had anything to do with. They deserve what they get.
The key disadvantage of writing sole-author papers is that it is extremely difficult to spot your
own omissions and mistakes. Your brain automatically corrects things sub-consciously and the
conscious part of your brain is not made aware of it. Everybody thinks they are a genius but you
often cannot recognise the flaws in your own reasoning or the flaws and oversights in your own
data or analysis. Finishing a paper and then completely ignoring it for a while helps you to find
mistakes.
Let the paper incubate for a while.
In biological sciences, generally you can find the information you need in an International
Journal or a book. Some research fields are much more dependent on grey literature. The two
subjects that come to mind are geography and geology (Martin and Ritchie 2020) where much of
the literature is in the form of government reports and reports of various organisations.
I think Day’s comment here is a bit dated. Today it is almost impossible to rewrite and
republish conference papers as papers in peer reviewed journals because of the “double
publication issue”. Avoid published conference papers because you are unlikely to be able
to reuse the material. Special issues of International Journals are OK. If you try and hide
that fact you are in big trouble. He also wrote it before predatory scientific journals
became a problem.
As a general rule use primary literature in Books or Journals as information sources. Publication
in primary literature is the aim of scientific publishing. Do not put your work in secondary
literature if it endangers your ability to publish it in a good journal or as a chapter in a book.
The literature that is not primary is often called Secondary, Grey or Non-archival Literature. The
term non-archival is the most apt name for it because if reflects the fact that it is not literature
that you would be able to normally find in a library. Examples of grey literature are government
reports, government reports not released publically, conference proceedings of a conference
which was attended by less than 30 people that was not published and is not on the internet.
Most university theses are grey literature because of limited accessibility.
from Sand-Jensen (2007). Note that Sand-Jensen has given proper credit for the figure.
It is also important to point out that Sand-Jensen is Scandinavian and is professionally using
English as a second language. Today most of your readers will be using English as a second
language. Keep that in mind.
Nearly all papers are submitted through the ScholarOne/Editorial Manager software produced
by Thomson-Reuters. If your abstract is even 1 word over the limit the software prevents you
from submitting your paper. Know the word limit beforehand. Sometimes this advice is no help
because when you get into ScholarOne/Editorial Manager you find they have changed the
word limit without changing the information in the Instructions to Authors. Do not try and
change the abstract off the top-of-your head while still in ScholarOne/Editorial Manager – that
is asking for mistakes.
• Every word counts so do not use superfluous phrases like “This study has shown ….”.
Databases search for info in Abstracts.
Highlights
• The latest fashion in publishing is to demand a set of highlights in point form.
• Highlights are extremely difficult to write. I think they are useless.
• If there is a highlights section in ScholarOne/Editorial Manager it is usually a rude
surprise because older copies of papers from the journal often do not have them and suddenly
when you are battling with Scholar-One you discover they want highlights. Damn them!
• Like in the case of the Abstract word limits the ScholarOne/Editorial Manager software
will not accept your highlights if they do not conform to the format.
• Usually only 5 highlights, and only 80 - 85 characters per highlight (including spaces)
• Highlights are one of the more useless recent innovations in publishing.
It is totally unacceptable for a student to hand their work to their supervisor for them to get it
published and think that is the end of the matter. The student does not learn the essentials of
being a functional researcher. That is why I make a point to teach students how to submit a
paper.
The student is normally the first author and needs to learn how to write papers by themselves. I
have had to resort to publishing a student’s work with me as first author only once in my career.
(He had got a government job and would not write the paper up).
The first hurdle to cross is to check that you have read the Instructions to authors properly.
Figures and Tables need to conform to what they want. Pointless to argue.
One of the most time consuming and mindless tasks is dealing with the references. You need to
check that every reference is in the text and in the reference list. Software to do this is not
perfect. Do not trust any of them.
All journals have reference formats of their own even when published by the same publisher.
There are some “standard” formats such as APA, Harvard and Vancouver (see Google Scholar,
under cite) but I do not know of a single journal that uses them exactly.
Reference Formats
1. Papers are listed with full journal names in alphabetical order. This format is the most
straightforward and should be used when you are actually writing the paper. References to
books must include the publisher & the city of publication (adding the country is optional).
2. Papers in alphabetical order using official journal abbreviations. These abbreviations are
often counter intuitive and so you have to check them from an official list. For journals without
an official ISI abbreviation use the full Journal title. Do not invent your own.
3. There are some journals that use a numbering system based on order of appearance e.g.
“Bloggs [1] and Smith and Jones [2] found different results from three previous studies others
such Ritchie, Scott and Dainty [4,5,6]”. This a terrible format to deal with. You have to write
the entire paper first and then replace names with numbers. Any edits create terrible messes.
Straight replacing names with numbers produces some ugly sentences such as “The validity of
assumptions by [2,4] have not been borne out [1,5,6]”. What on earth does that mean? That is
terrible expression. Rewrite like the example above to make it readable. It is much better to be
able to read in the text who it was who did or said what.
Some journals, particularly new ones, may have no official abbreviation. Just use the full journal
name. The websites above only list currently published journals. It can be very hard to find the
correct abbreviations of old journals no longer published and conversely you might have the
abbreviation on your copy of the paper but cannot find the full journal name.
Journals published by political entities that no longer exist can be very troublesome. For
example, old Soviet journals.
About 2 to 3 months after submission of a paper you get sent an email from the editor. Outright
acceptance is extremely rare so you need a tough hide to deal with the referee’s comments. Do
not sulk too long. The sooner you rewrite, correct and respond to all the referee’s profound and
helpful comments on the paper the better chances you have of getting it accepted.
Rule #1 respond to all the queries by your esteemed Editor and Referees no matter how inane or
stupid they seem. It is pretty standard to get one or two sympathetic referee’s reports and
one complete arsehole. That is life. The more sensible journal editors understand that.
We have just got to get a move on and complete the revision of our paper.
The Abstract
Remember what I said about the Abstract. It is the first thing people read so if your Abstract is
bad, generally they do not read any more of the paper. The Abstract was the first thing a referee
reads and if they did not like it things go downhill from there. Always go over your Abstract
because it is likely that it was the start of your problems with the referee.
When you revise a paper give the Abstract some serious attention even if the referees did not
comment on it. If you find things that annoy the referees in the rest of the paper and you revise
the body of the paper and not the abstract you are asking for trouble. When you have revised
your paper make sure the Abstract tallies with the new version of the paper. This is easy to
overlook.
The Introduction
You must have a clear statement of what is the problem your paper is addressing and why it is
important. Referees do not like Introductions that do not clearly state what the paper is about.
Did you make the aims of the paper clear in the last paragraph? You need only put in a single
sentence about what your paper has shown. This statement must be obvious not implied.
If the Referee starts pointing out “missing” papers, books etc. You can usually work out who
the Referee is. Make sure you cite their profoundly important papers.
It is a simple and common mistake to bring up points in the Introduction that are not dealt with in
the paper. Leave them out or move them to the Discussion as profound future avenues of
investigation.
Do not make the Introduction too heavy. Go easy on theory and equations. I must admit this is
a bad habit of mine.
As pointed out above, you may need to say what your paper is not about.
For example “Extrasolar planets have been found orbiting G, K and M stars but here we do not
discuss planets of K stars because as yet so few are documented”.
The Introduction should demonstrate clear evidence that you critically read the literature and
identified the problem. If there is a lot of criticism of your Introduction you need to give it close
attention as you have not set up your problem properly.
They rejected my paper! Sulk for a few days then rewrite it and send it somewhere else.
I am the editor and I have rejected your paper. Don’t even think you can win an argument with
me.
If you go to a conference you are often offered giving a poster presentation rather than giving a
talk. Often giving a poster is often a better way for a student to present their work. A poster over
a talk also has the advantage that a poster presentation does not present difficulties about “double
publication” like a published conference paper. As pointed out above you need to be wary of the
supposed benefits of published conference papers. Career-wise they are not as useful as you
might think.
Phongiarus, N.,
Suyaphat C., Naiyana
Srichai, N., Ritchie,
R.J. Growth of
photosynthetic
bacteria
(Rhodopseudomonas
palustris) on cooking
oils. Asia Pacific
Phycological Forum,
Hotel Pullman Kuala
Lumpur Bangsar,
Kuala Lumpur, 8-
13th October 2017.
SB13, p 258.
The person presenting the poster should be underlined and do not forget
full address and emails.
How to
Construct the
Poster
How to Publish 2021Version - 71
Arsenic toxicity in a Photosynthetic Bacterial Symbiont of Wolffia
aarhiza.
Raymond J. RITCHIE 1 and Siriporn NAKPHET1
1 Technology and Environment, Prince of Songkla University-Phuket, Kathu, 83120,
Thailand,
Corresponding author: Raymond J. RITCHIE, E-mail:
raymond.ritchie@alumni.sydney.edu.au, raymond.r@phuket.psu.ac.th
Summary
An arsenic resistant nitrogen fixing photosynthetic bacterium found to live inside Wolffia aarhiza plants has been cultured and identified as a
Rhodopseudomonad species, most likely a strain of Rhodobacter capsulatus. It has BChl a as its primary photosynthetic pigment and has
spectral properties typical of a Rhodopseudomonad. Blue-diode-based PAM (Pulse Amplitude Modulation) technology can be used to
measure the photosynthetic electron transport rate (ETR) of the organism. The absorptance of the Rhodobacter films on glass fibre discs was
measured and used to calculate actual ETR as mol e- g-1 BChl a s-1. ETR vs. Irradiance (E) curves fitted the waiting-in-line model (ETR =
(ETRmax E/Eopt) exp (1-E/Eopt)). Yield (Y) was only ≈ 0.3 to 0.4. Rhodobacter saturates at about 250 to 350 µmol photons m-2 s-1 or ≈ 15%
sunlight and shows photoinhibition at high irradiances (overall Eopt was 298 7.36 μmol quanta m-2 s-1; ETRmax = 642 10.6 μmol e- g-1 BChl
a s-1; Alpha (α) = 6.05 0.200 e- photon-1 m 2 g-1 BChl a). Photosynthetic performance was much worse in Low-P medium (n = 108, overall
Eopt 158 15.4 μmol quanta m-2 s-1; ETRmax = 194 13.5 μmol e- g-1 BChl a s-1; α = 3.30 0.400 e- photon-1 m 2 g-1 BChl a). Rhodobacter is
resistant to As (V) toxicity up to at least 1 mol m-3 in high and low P medium. The Ki for As(III) in High and Low-P are not significantly different:
overall mean was 497 100 mmol m-3 but there is a threshold effect below about 200 mmol m-3 As(III). Fe(II) and As(III) did not appear to act
as electron sources but thiosulphate did act as an electron source for photosynthesis. Al(OH)3 and Hg2+ are common aquaporin channel
blocking agents but neither acted as protectants against As(III) toxicity.
Keywords: Photosynthetic bacteria, Rhodobacter, anoxygenic photosynthesis, integrating sphere spectrophotometry, PAM fluorometry.
Thailand,
Corresponding author: Raymond J. RITCHIE, E-mail:
raymond.ritchie@alumni.sydney.edu.au, raymond.r@phuket.psu.ac.th
Summary
An arsenic resistant nitrogen f ixing photosynthetic bacterium f ound to live inside Wolffia aarhiza plants has been cultured and identif ied as a
Rhodopseudomonad species, most likely a strain of Rhodobacter capsulatus. It has BChl a as its primary photosynthetic pigment and has spectral
properties typical of a Rhodopseudomonad. Blue-diode-based PAM (Pulse Amplitude Modulation) technology can be used to measure the
photosynthetic electron transport rate (ETR) of the organism. The absorptance of the Rhodobacter f ilms on glass f ibre discs was measured and used
to calculate actual ETR as mol e- g -1 BChl a s -1. ETR vs. Irradiance (E) curves f itted the waiting-in-line model (ETR = (ETRmax E/Eopt ) exp (1-E/Eopt )).
Yield (Y) was only ≈ 0.3 to 0.4. Rhodobacter saturates at about 250 to 350 µmol photons m-2 s -1 or ≈ 15% sunlight and shows photoinhibition at high
irradiances (overall Eopt was 298 7.36 μmol quanta m-2 s -1; ETRmax = 642 10.6 μmol e- g -1 BChl a s -1; Alpha (α) = 6.05 0.200 e- photon-1 m2 g -1
BChl a). Photosynthetic perf ormance was much worse in Low-P medium (n = 108, overall Eopt 158 15.4 μmol quanta m-2 s -1; ETRmax = 194 13.5
μmol e- g -1 BChl a s -1; α = 3.30 0.400 e- photon-1 m2 g -1 BChl a). Rhodobacter is resistant to As (V) toxicity up to at least 1 mol m-3 in high and low P
medium. The Ki f or As(III) in High and Low-P are not signif icantly different: overall mean was 497 100 mmol m-3 but there is a threshold ef f ect below
about 200 mmol m-3 As(III). Fe(II) and As(III) did not appear to act as electron sources but thiosulphate did act as an electron source f or
photosynthesis. Al(OH)3 and Hg 2+ are common aquaporin channel blocking agents but neither acted as protectants against As(III) toxicity.
Keywords: Photosynthetic bacteria, Rhodobacter, anoxygenic photosynthesis, integrating sphere spectrophotometry, PAM f luorometry.
Wolffia arrhiza This mm ruler gives you some idea of its size.
You can do experiments on a real flowering plant on a
very small scale, even in a single Eppendorf Tube (1.4
Fig. 1 Wolffia is a genus of very simple flowering plants Rhodobacter - H2S and SO32- as e- sources
Flower
ml).
Generation time ≈ -5 days. (Angiosperms). They are the smallest known flowering Control - no electron or carbon source
Control Fit
Stamen
& Anthers Daughter plants. The very simple anatomy of Wolffia helps in the Hydrogen Sulphide data
Hydrogen Sulphide 0.3 mM Fit
frond Thiosulphate data
analysis and interpretation of arsenic uptake studies. No roots,
ETR capsulatus. It is easily grown in culture in PM medium using thiosulphate (300 mmol m-3). H 2S marginally increased
the photosynthetic ETR and so was being used as an
ETR (μmol g-1 BChl a s-1)
Equation (ETR max = 469 30.1 μmol e - g-1 BChl a s-1 ; Eopt = 0. mM Arsenite Fit
1.0 mM Arsenite data
263 24.8 μmol quanta m -2 s-1 ; Alpha (α) = 4.843 0.553 e-
ETR (µmol g-1 BChl a s-1)
1 mM Arsenite Fit
0. mM Hg + 1 mM Arsenite
Hg + Asenite Fit
compared to cells exposed to arsenite (As(III). Hg2+ had no
effect on ETR compared to the control. 1.0 mol m -3 As (III)
was inhibitory by about 50% on ETR max but did not appear to
have much effect on the optimum irradiance (E opt ) about 200
PPFD Irradiance (µmol photon m-2 s-1)
μmol photon m -2 s-1 . Hg2+ had no protective effect against
As(III) toxicity. Similar experiments using Al(OH)3 as a Fig. 6 Photosynthetic electron transport rate of
potential channel blocker also showed no effective channel Rhodobacter incubated in electron source-free PM
blocking behaviour to prevent As(III) toxicity. medium (control) and supplied with 0.2 mol m-3
As(III) and 1 mol m-3 As(III). As(III) did not
PPFD Irradiance (µmol photon m -2 s-1) increase the photosynthetic ETR and so As(III) could
Rhodobacter- Arsenite and Arsenate in PM medium
Rhodobacter - Low Phosphate Medium
Arsenate and Arsenite
Fig. 4 The toxicity of As(III) and As(V) are quite different not be used as an electron source for photosynthesis
after 3h exposure to arsenic. As(III) is obviously toxic and (cf. Ref 5)
ETR (µmol e- g-1 BChl a s-1)
ETR (µmol e- g-1 BChl a s-1)
Arsenate (AsV)
Arsenite (AsIII)
y = 0.389x + 583.5
R² = 0.896
r = 0.9466 Put in all the bits,
mmol m -3 ) but with a threshold effect and is unaffected by
[Phosphate]. Rhodobacter is highly resistant to As(V) and
Conclusions
• Arsenite (As(III)) is toxic but Arsenate (As(V)) has no short-term inhibitory effects. Response to As(V) is different under high P compared to low-P conditions. Wombat.
• Al(OH)3 and Hg 2+ are common aquaporin channel blocking agents but neither acted as protectants against As(III) toxicity.
• As(III) does not act as an electron source for photosynthesis in Rhodobacter.
References
(1) Ritchie RJ, Runcie JW (2013). Measurement of the Photosynthetic Electron Transport Rate in an Anoxygenic Photosynthetic Bacterium Afifella (Rhodopseudomonas) marina using PAM Fluorometry. Photochem. Photobiol. 89: 370-383.
(2) Ritchie RJ (2013). The Use of Solar Radiation by a Photosynthetic Bacterium Living as a Mat or in a Shallow Pond or Flatbed Reactor. Photochem. Photobiol. 89:1143-1162 (DOI: 10.1111/php.12124).
(3) Ritchie RJ, Mekjinda N (2015). Measurement of photosynthesis using PAM technology in a purple sulphur bacterium Thermochromatium tepidum (Chromatiaceae). Photochem. Photobiol. 91: 350-358. DOI: 10.1111/php.12413
(4) Ritchie, Raymond J. and Mekjinda, Nutsara (2016). Arsenic Toxicity in the Water Weed Wolffia arrhiza Measured using Pulse Amplitude Modulation Fluorometry (PAM) Measurements of Photosynthesis. Ecotoxicology and Environmental Safety 132: 178 – 187;
doi:10.1016/j.ecoenv.2016.06.004
(5) Kulp, T.R. et al., (2008.) Arsenic(III) fuels anoxygenic photosynthesis in hot spring biofilms from Mono Lake, California. Science 321, 967 – 970, doi:10.1126/science.1160799
Thailand,
Corresponding author: Raymond J. RITCHIE, E-mail:
raymond.ritchie@alumni.sydney.edu.au, raymond.r@phuket.psu.ac.th
Summary
An arsenic resistant nitrogen f ixing photosynthetic bacterium f ound to live inside Wolffia aarhiza plants has been cultured and identif ied as a
Rhodopseudomonad species, most likely a strain of Rhodobacter capsulatus. It has BChl a as its primary photosynthetic pigment and has spectral properties
typical of a Rhodopseudomonad. Blue-diode-based PAM (Pulse Amplitude Modulation) technology can be used to measure the photosynthetic electron
transport rate (ETR) of the organism. The absorptance of the Rhodobacter f ilms on glass f ibre discs was measured and used to calculate actual ETR as mol
e- g -1 BChl a s -1. ETR vs. Irradiance (E) curves f itted the waiting-in-line model (ETR = (ETRmax E/Eopt ) exp (1-E/Eopt )). Yield (Y) was only ≈ 0.3 to 0.4.
Rhodobacter saturates at about 250 to 350 µmol photons m-2 s -1 or ≈ 15% sunlight and shows photoinhibition at high irradiances (overall Eopt was 298 7.36
μmol quanta m-2 s -1; ETRmax = 642 10.6 μmol e- g -1 BChl a s -1; Alpha (α) = 6.05 0.200 e- photon-1 m2 g -1 BChl a). Photosynthetic perf ormance was much
worse in Low-P medium (n = 108, overall Eopt 158 15.4 μmol quanta m-2 s -1; ETRmax = 194 13.5 μmol e- g -1 BChl a s -1; α = 3.30 0.400 e- photon-1 m2 g -1
BChl a). Rhodobacter is resistant to As (V) toxicity up to at least 1 mol m-3 in high and low P medium. The Ki f or As(III) in High and Low-P are not
signif icantly different: overall mean was 497 100 mmol m-3 but there is a threshold ef f ect below about 200 mmol m-3 As(III). Fe(II) and As(III) did not appear
to act as electron sources but thiosulphate did act as an electron source f or photosynthesis. Al(OH)3 and Hg 2+ are common aquaporin channel blocking
agents but neither acted as protectants against As(III) toxicity.
Keywords: Photosynthetic bacteria, Rhodobacter, anoxygenic photosynthesis, integrating sphere spectrophotometry, PAM f luorometry.
Fig. 1 Wolffia is a genus of very simple flowering plants Rhodobacter - H2S and SO32- as e- sources
Wolffia arrhiza
Flower
Stamen
This mm ruler gives you some idea of its size.
You can do experiments on a real flowering plant on a
very small scale, even in a single Eppendorf Tube (1.4
ml).
Generation time ≈ -5 days.
Final Steps
(Angiosperms). They are the smallest known flowering
plants. The very simple anatomy of Wolffia helps in the
Control - no electron or carbon source
Control Fit
Hydrogen Sulphide data
Hydrogen Sulphide 0.3 mM Fit
Thiosulphate data
& Anthers analysis and interpretation of arsenic uptake studies. No roots,
Frond
~ 1 mm stems or leaves or xylem and phloem. The very small size is
Adaxial Bad news:
Despite what you would think also an advantage in the laboratory.
Photosynthesis in Rhodobacter
Fig. 2 Like the water fern Azolla, Wolffia has endosymbiotic Fig. 5 Photosynthetic electron transport rate of
bacteria capable of N-fixation. Wolffia has an endosymbiotic Rhodobacter incubated in electron source-free PM
Yield
N-fixing anoxygenic photosynthetic bacterium , Rhodobacter medium (control) and supplied with Na2S or
Yield Fit
ETR
summary
capsulatus. It is easily grown in culture in PM medium using thiosulphate (300 mmol m-3). H2S marginally increased
the photosynthetic ETR and so was being used as an
ETR (μmol g-1 BChl a s-1)
Control Fit
Equation (ETR max = 469 30.1 μmol e - g-1 BChl a s-1 ; Eopt = 0. mM Arsenite Fit
1.0 mM Arsenite data
263 24.8 μmol quanta m -2 s-1 ; Alpha (α) = 4.843 0.553 e-
ETR (µmol g-1 BChl a s-1)
1 mM Arsenite Fit
0. mM Hg + 1 mM Arsenite
Hg + Asenite Fit
font.(Common Thai fonts will
compared to cells exposed to arsenite (As(III). Hg2+ had no
effect on ETR compared to the control. 1.0 mol m -3 As (III)
was inhibitory by about 50% on ETR max but did not appear to
not print properly on printers!).
have much effect on the optimum irradiance (E opt ) about 200
μmol photon m -2 s-1 . Hg2+ had no protective effect against
PPFD Irradiance (µmol photon m-2 s-1)
Arsenate (AsV)
Conclusions
• Arsenite (As(III)) is toxic but Arsenate (As(V)) has no short-term inhibitory effects. Response to As(V) is different under high P compared to low-P conditions.
well. That was a mistake.
• Al(OH)3 and Hg 2+ are common aquaporin channel blocking agents but neither acted as protectants against As(III) toxicity.
• As(III) does not act as an electron source for photosynthesis in Rhodobacter.
References
•Make a pdf of the grouped
(2) Ritchie RJ (2013). The Use of Solar Radiation by a Photosynthetic Bacterium Living as a Mat or in a Shallow Pond or Flatbed Reactor. Photochem. Photobiol. 89:1143-1162 (DOI: 10.1111/php.12124).
(3) Ritchie RJ, Mekjinda N (2015). Measurement of photosynthesis using PAM technology in a purple sulphur bacterium Thermochromatium tepidum (Chromatiaceae). Photochem. Photobiol. 91: 350-358. DOI: 10.1111/php.12413 poster and get the pdf printed.
(1) Ritchie RJ, Runcie JW (2013). Measurement of the Photosynthetic Electron Transport Rate in an Anoxygenic Photosynthetic Bacterium Afifella (Rhodopseudomonas) marina using PAM Fluorometry. Photochem. Photobiol. 89: 370-383.
(4) Ritchie, Raymond J. and Mekjinda, Nutsara (2016). Arsenic Toxicity in the Water Weed Wolffia arrhiza Measured using Pulse Amplitude Modulation Fluorometry (PAM) Measurements of Photosynthesis. Ecotoxicology and Environmental Safety 132: 178 – 187;
doi:10.1016/j.ecoenv.2016.06.004
(5) Kulp, T.R. et al., (2008.) Arsenic(III) fuels anoxygenic photosynthesis in hot spring biofilms from Mono Lake, California. Science 321, 967 – 970, doi:10.1126/science.1160799
Bad news:
Despite what you would think
Wolffia is not easy to grow!
- .
That is why I have no sympathy for Prof Bullfrog and Prof Dracula who bully people into
putting their names on their papers.
A CLASSIC CASE OF SCIENTIFIC FRAUD. Expelled from Scripps Institute where she was
doing a Post-Doctoral for fabricating sea urchin survey data on a date when she was away. As is
usual, she was caught accidentally. She was convinced that she was surrounded by gullable
fools and she could do anything she liked. Some smart people are like that. The trouble with that
attitude is that “idiots” sometimes notice things and it starts a snowballing effect or a collapsing
house of cards.
She disappeared for many years then emerged out of the woodwork. Apparently she has lost her
marbles but still finds gullible people to exploit. Predictably enough the UN is one of her
victims. Rather shamefully she still tries to use her University of Sydney Honours and PhD
degrees for credibility.
Very smart but Mad-Bad-and-Dangerous-to-Know.
http://en.heartfulness.org/blog/2015/05/17/heartfulness-
elizabeth/
http://omegawellbeing.org/our-team/elizabeth-denley/
All authors to papers in the International Journal of Cardiology must adhere to the following
principles:
1. That the corresponding author has the approval of all other listed authors for the submission
and publication of all versions of the manuscript.
2. That all people who have a right to be recognized as authors have been included on the list of
authors and everyone listed as an author has made an independent material contribution to the
manuscript.
3. That the work submitted in the manuscript is original and has not been published elsewhere
and is not presently under consideration of publication by any other journal other than in oral,
poster or abstract format.
4. That the material in the manuscript has been acquired according to modern ethical standards
and has been approved by the legally appropriate ethical committee.
5. That the article does not contain material copied from anyone else without their written
permission.
6. That all material which derives from prior work, including from the same authors, is properly
attributed to the prior publication by proper citation.
7. That the manuscript will be maintained on the servers of the Journal and held to be a valid
publication by the Journal only as long as all statements in these principles remain true.
8. That if any of the statements above ceases to be true the authors has a duty to notify the
Journal as soon as possible so that the manuscript can be withdrawn.
* Not a bad set of general guidelines.
Comment: all well and good but in reporting scientific fraud I can assure the readers that it
is usually the end of your career.
The paradox of the super smart that really do not need to commit fraud but do anyway.
• These people are usually caught by accident.
• Their behaviour is basically psychopathic. They think they can get away with anything.
They often have done so since childhood and will continue until perhaps one day they are
caught (if ever).
• Their arrogance is what gets them caught. They become careless. If Mark Spector had
dipped his blue Pentel pen in a solution laced with 32P and not radioiodine (131I) he would
never have been caught and discredited. He would now be at least Prof Mark SPECTOR.
• There is a snowball effect. Once suspicions are aroused all sorts of other things fall into
place. A single falsified survey date or a single fudged electrophoresis gel is enough to
get people looking at all other work by that individual. Things once ignored or skipped
over are suddenly remembered and seen to fit a pattern. Because of the
interconnectedness of science the whole House-of-Cards falls.
• In my opinion they do it because they can and despise the rest of mankind.