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The synapse is the point of communication between neurons – from (presynaptic) neurons to
(postsynaptic) effector cells (e.g. muscle and glandular cells).
Synaptic control of neuronal activity provides the complex, Brain function has regional
elaborate, subtle and flexible mechanisms by which the brain specificity
is able to process information.
Brain function has neurochemical
When chemical synaptic transmission goes wrong, ⇒nervous (neurotransmitter) specificity.
system malfunction. Defective NT is the root cause of a large
number of neurological and psychiatric disorders
Synaptic neurotransmission is also the point at which we can
modify neuronal function with drugs. Mechanism of transmitter
release- (peptides)
The rapid release is possible because some of the vesicles are “docked “ to the plasma
membrane and primed for fusion.
Only a small proportion of vesicles fuse with plasma membrane in response to each AP
For nerve terminal to respond rapidly and repeatedly, vesicles need to be replenished very
quickly after discharged. Vesicles are retrieved from plasma membrane by endocytosis
Secretory granules and synaptic vesicles are frequently observed in the same axon terminals.
These different neurotransmitters are released under different conditions
Curare- poison arrows /muscle relaxant in surgery– block ACh receptors on skeletal muscle
Barbiturates and tranquilizers e.g. diazepam (valium) – bind to GABA receptors ⇒potentiate inhibitory action of
GABA by allowing lower[GABA] to open Cl- channels
Fluozetine (Prozac)- blocks uptake of 5-HT
G-coupled receptors :
NT binds to receptors ⇒activate small G-proteins ⇒activate ‘effector’ proteins (either G-protein –gated ion
channels or 2nd messengers).
Synapses with transmitter-gated channels carry the bulk of specific information that is processed by the nervous
system. But the effectiveness of these are modified by the many synapses with G-protein coupled receptors.
The same NT can give different postsynaptic actions depending on what receptor it binds to e.g.
ACh in heart – coupled by a G -protein to a K+ channel⇒ hyperpolarises the heart muscle fibre slows heartbeat
ACh in skeletal muscle- binds to ACh-gated ion channel permeable to Na+⇒rapid depolarises skeletal
muscle⇒contraction
After neurotransmitter has been secreted it is rapidly removed- either destroyed by specific enzymes in
synaptic cleft or taken up presynaptic nerve terminal or by surrounding glial cells.
Catecholamines
Neurotransmitters dopamine, noradrenalin and adrenalin are synthesised from the amino acid tyrosine
Catecholaminergic neurons- found in regions of nervous system involved with regulation of movement, mood,
attention and visceral function.
Tyrosine has an active transport mechanism for uptake into the brain, shared with other large neutral amino acids.
Removal of catecholamine neurotransmitters from synaptic cleft is by selective uptake back via Na+-dependent
transporter into presynaptic terminal axon terminals.
Amphetamine increases release of DA whilst cocaine blocks uptake – therefore both prolong DA action
In axon terminal- catecholamines may be reloaded into synaptic vesicle for reuse or enzymatically degraded by
enzymes esp. Monoamine oxidase (MAO) on outer mitochondrial membrane
MAO-A removes noradrenalin and 5-HT,
MAO-B removes dopamine
(MAO enzymes are present in the liver and gut, and provide protection from exogenous retroactive chemicals)
MAO inhibitors have been used (a) in the treatment of: Depression and Parkinson’s disease and (b) to increase levels
of NT- BUT MAO-A inhibition can cause hypertensive crisis (the “cheese effect”) due to the neuroactive effects of
dietary amines such as tyramine, found in cheese, marmite etc
Parkinson’s disease
Tremor, rigidity, akinesia (slowing of movement, postural changes. No sensory loss and cognitive function is
preserved until late stages.
• Primary pathology- progressive cell degeneration of pigmented dopaminergic cells in the substantia nigra
which innervate the striatum (The striatum can be considered as a system that inhibits motor function).
Dopamine, via its effects on D2 receptors, inhibits the cells of the striatum⇒diminishes their inhibitory
activity.
As well as output neurons and dopaminergic terminals, the striatum contains cholinergic interneurons that
have an excitatory effect (i.e. opposite to that of dopamine) on the striatum;
⇒If dopamine is lost, decreasing ACh activity will tend to restore the balance.
⇒So an antagonist at ACh receptors has similar effects to an agonist at dopamine receptors (Anticholinergic
drugs blocking muscarinic receptors (a subtype of ACh receptors) can alleviate Parkinsonian symptoms Old
treatments for Parkinson’s used anticholinergic agents (belladonna alkaloids))
The pathway from the subs. nigra to the striatum is only one of several important dopaminergic systems in
the brain innervating the:
• striatum (part of the basal ganglia - controlling motor function)
• cortical and limbic regions (emotion, memory, complex behaviours, addiction, psychosis)
• pituitary gland (hormonal secretion)
The antipsychotic drugs are thought to act on dopamine receptors in cortical and/or limbic regions to improve
some of the symptoms of schizophrenia. Their Parkinsonian side effects are due to antagonism of striatal
dopamine receptors. There effects on dopamine receptors in the pituitary that control prolactin secretion can
result in the overproduction of prolactin and gynaecomastia (enlarged breasts) and galactorrhoea (milk
secretion) – even in males!
5-HT systems:
Diffuse projection from brainstem (raphe nuclei in the reticular formation – the “reticular activating system”) to
widespread areas of forebrain, particularly the cortex.
The function of 5-HT systems include:
• Consciousness/arousal
• Circadian rhythms
• Mood
• Regulating aggression
Drugs that increase synaptic 5-HT levels are used in the treatment of depression. These include the tricyclic
antidepressants and the newer specific serotonin reuptake inhibitors (SSRIs) like fluoxetine (Prozac), that block
reuptake removal of 5-HT into the presynaptic terminal.
Tryptophan, as a precursor of 5-HT, has also been used as an antidepressant, as have MAO (A) inhibitors that
prevent 5-HT breakdown
Amino acids, Glutamate, Glycine and GABA serve as NTs at most CNS synapses.
Glutamate
Most common excitatory transmitter in brain.
Very efficient and specific uptake processes remove glutamate into neurons and glia.
Found throughout the brain, notably as the transmitter in large pyramidal neurons (e.g. motor
neurons) of the cortex that project to other regions of the brain or spinal cord.
In the CNS, single neurons can receive inputs from 1000s of other neurons and can in turn synapse on
may thousands of other selves
The average motor neuron in the spinal cord has several thousand nerve terminals synapsing on its cell
body + dendrites. The motor neuron must combine information from these sources and react either by
firing APs along its axon or remaining quiet.
Any single NT can have different effects depending on what receptor it can bind to.
Divergence =The ability of one NT to activate more than one subtype of receptor and cause more than one
type of postsynaptic response. Divergence may occur at any stage in the cascade of transmitter (e.g.
which G-proteins and which effector systems are activated)
Convergence. multiple transmitters activating their own receptor type can converge on the same effector
system (again can occur at any stage in the signal cascade)
Neurons integrate divergent and convergent signalling systems
Recommended Reading:
Anatomy & Physiology: The Unity of form of function 463-468- – not extensive enough on pathways and
neurochemistry
Neuroscience Chapter 6 – admittedly a bit deep but it’s all there.
Neuroscience at a Glance 24-25, 106, 122.
Fundamentals of Psychopharmacology by Brain Leonard also has this material in Chapter 1 but the book
isn’t particularly user friendly for Level 1.
Neuroscience: exploring the brain –Chapter 5 and 6- good general overview with additional ‘facts of special
interest’