Referencia (S3)

Estandar Concentración (Ug/mL)

Replica Zona 1 (mm) Zona 3 (mm)
1 15.9 15.4
S1 8.03 2 15.8 15.7
3 15.5 15.5
1 15.6 15.4
S2 10.03 2 15.5 15.3
3 15.5 15.2
1 15.4 15.6
S4 15.68 2 15.6 15.4
3 15.9 15.5
1 15.4 15.4
S5 19.63 2 15.4 15.5
3 15.7 15.6
1 15.5 15.3
Muestra Desconocida 2 15.5 15.2
3 15.4 15.6

Referencia
Estandar Concentración (Ug/mL)
Replica Zona 1 (mm) Zona 3 (mm)
1 15.9 15.4
S1 8.03 2 15.8 15.7
3 15.5 15.5
1 15.6 15.4
S2 10.03 2 15.5 15.3
3 15.5 15.2
1 15.4 15.6
S4 15.68 2 15.6 15.4
3 15.9 15.5
1 15.4 15.4
S5 19.63 2 15.4 15.5
3 15.7 15.6
1 15.5 15.3
Muestra Desconocida 2 15.5 15.2
3 15.4 15.6

Valor P = 15.522222222222

Estandar Concentración (Ug/mL) Log Concentración Media corregida

20
S1 8.03 0.904715545278681 14.15555556
18

16
S2 10.03 1.00130093302042 15.13333333
14

12
egida
 20

18

16
S2 10.03 1.00130093302042 15.13333333
14

12

Media corregida
S3 12.54 1.0982975364947 15.502222222222
10

8
S4 15.68 1.19534605834842 16.64444444
6

S5 19.63 1.29292029960001 17.35555556 4

Muestra Desconocida 13.56666667 0

0.85 0
encia (S3) S1, S2, S3, S4
Zona 5 (mm) Zona 2 (mm) Zona 4 (mm) Zona 6 (mm)
15.6 14.5 14.3 13.8
16 14.4 14.3 14.7
Determinació
15.6 13.9 14.4 14.4 P𝑒𝑠𝑜 𝑔𝑒𝑛𝑡𝑎𝑚𝑖𝑐𝑖𝑛𝑎 𝑠𝑢𝑙𝑓𝑎𝑡𝑜=36,9 𝑚𝑔
15.3 14.6 15.3 15.1 𝑃𝑢𝑟𝑒𝑧𝑎=593 𝑈𝑙/𝑚𝑔
15.4 14.7 15.1 15.5 % 𝐻𝑢𝑚𝑒𝑑𝑎𝑑=8,1%
𝑃𝑒𝑠𝑜 𝐺𝑆=36,9 𝑚𝑔∗593 𝑈𝑙/𝑚𝑔∗1𝑢𝑔/641𝑈𝑙∗(
15.1 14.7 15.2 14.6 𝑠𝑜𝑙𝑢𝑐𝑖ó𝑛 𝑚𝑎𝑑𝑟𝑒=(31,37 𝑢𝑔)/(50 𝑚𝐿)=0,627
15.8 16.5 17 16.6 𝐸𝑠𝑡𝑎𝑛𝑑𝑎𝑟 1=0,627 𝑚𝑔/𝑚𝐿∗(128 𝑢𝐿)/(10 𝑚𝐿
15.5 16.5 16.7 16.5 𝐸𝑠𝑡𝑎𝑛𝑑𝑎𝑟 2=0,627 𝑚𝑔/𝑚𝐿∗(160 𝑢𝐿)/(10 𝑚𝐿
15.6 16.8 16.7 17.1 𝐸𝑠𝑡𝑎𝑛𝑑𝑎𝑟 3=0,627 𝑚𝑔/𝑚𝐿∗(500 𝑢𝐿)/(25 𝑚𝐿
𝐸𝑠𝑡𝑎𝑛𝑑𝑎𝑟 4=0,627 𝑚𝑔/𝑚𝐿∗(250 𝑢𝐿)/(10 𝑚𝐿
15.3 17.2 17.2 17.3 𝐸𝑠𝑡𝑎𝑛𝑑𝑎𝑟 5=0,627 𝑚𝑔/𝑚𝐿∗(313 𝑢𝐿)/(10 𝑚𝐿
15.3 17.2 17.6 17.5
15.6 17.2 17.5 17
15.4 13.3 13.8 13.5
15.1 13.8 13.7 13.7
15.8 13.2 13.1 13.1

Referencia (S3) S1, S2, S3, S4

Zona 5 (mm) Media (mm) SD %RSD Zona 2 (mm) Zona 4 (mm) Zona 6 (mm)
15.6 14.5 14.3 13.8
16 15.666666667 0.2 1.276595745 14.4 14.3 14.7
15.6 13.9 14.4 14.4
15.3 14.6 15.3 15.1
15.4 15.366666667 0.158113883 1.02894067 14.7 15.1 15.5
15.1 14.7 15.2 14.6
15.8 16.5 17 16.6
15.5 15.588888889 0.169148193 1.085056119 16.5 16.7 16.5
15.6 16.8 16.7 17.1
15.3 17.2 17.2 17.3
15.3 15.466666667 0.141421356 0.914362217 17.2 17.6 17.5
15.6 17.2 17.5 17
15.4 13.3 13.8 13.5
15.1 15.422222222 0.210818511 1.366978816 13.8 13.7 13.7
15.8 13.2 13.1 13.1

Regresión lineal
20

18
f(x) = 8.15200160391991 x + 6.80311742079372
16 R² = 0.983843241803499

14

12
egida
 20

18
f(x) = 8.15200160391991 x + 6.80311742079372
16 R² = 0.983843241803499

14

12
Media corregida

10

0
0.85 0.9 0.95 1 1.05 1.1 1.15 1.2 1.25 1.3 1.35

Log concentración

Se acepta ya que el R^2 nos dio 0,9838, el cual es mayor a

0,95
 Determinación de las concentraciones
𝑛𝑡𝑎𝑚𝑖𝑐𝑖𝑛𝑎 𝑠𝑢𝑙𝑓𝑎𝑡𝑜=36,9 𝑚𝑔 1 𝑚𝑔=641 𝑈𝑙
=593 𝑈𝑙/𝑚𝑔
𝑒𝑑𝑎𝑑=8,1%
𝑆=36,9 𝑚𝑔∗593 𝑈𝑙/𝑚𝑔∗1𝑢𝑔/641𝑈𝑙∗((100−8,1))/100=31,37 𝑚𝑔
𝑛 𝑚𝑎𝑑𝑟𝑒=(31,37 𝑢𝑔)/(50 𝑚𝐿)=0,627 𝑚𝑔/𝑚𝐿
𝑎𝑟 1=0,627 𝑚𝑔/𝑚𝐿∗(128 𝑢𝐿)/(10 𝑚𝐿)∗(1000 𝑢𝑔)/(1 𝑚𝑔)∗(1 𝑚𝐿)/(1000 𝑢𝐿)= 𝟖,𝟎𝟐𝟓𝟔 𝒖𝒈/𝒎𝑳
𝑎𝑟 2=0,627 𝑚𝑔/𝑚𝐿∗(160 𝑢𝐿)/(10 𝑚𝐿)∗(1000 𝑢𝑔)/(1 𝑚𝑔)∗(1 𝑚𝐿)/(1000 𝑢𝐿)= 𝟏 𝟎 ,𝟎 𝟑 𝟐 𝒖𝒈/𝒎𝑳
𝑎𝑟 3=0,627 𝑚𝑔/𝑚𝐿∗(500 𝑢𝐿)/(25 𝑚𝐿)∗(1000 𝑢𝑔)/(1 𝑚𝑔)∗(1 𝑚𝐿)/(1000 𝑢𝐿)= 𝟏 𝟐 ,𝟓 𝟒 𝒖𝒈/𝒎𝑳
𝑎𝑟 4=0,627 𝑚𝑔/𝑚𝐿∗(250 𝑢𝐿)/(10 𝑚𝐿)∗(1000 𝑢𝑔)/(1 𝑚𝑔)∗(1 𝑚𝐿)/(1000 𝑢𝐿)= 𝟏 𝟓 ,𝟔 𝟕 𝟓 𝒖𝒈 /𝒎𝑳
𝑎𝑟 5=0,627 𝑚𝑔/𝑚𝐿∗(313 𝑢𝐿)/(10 𝑚𝐿)∗(1000 𝑢𝑔)/(1 𝑚𝑔)∗(1 𝑚𝐿)/(1000 𝑢𝐿)= 𝟏 𝟗 ,𝟔 𝟐 𝟓 𝟏 𝒖𝒈/𝒎𝑳

Media corregida
Media (mm) SD %RSD

14.3 0.28284271 1.97792107 14.1555555555556

14.9777778 0.334581 2.2338494 15.1333333333333

16.7111111 0.22047928 1.31935737 16.6444444444444

17.3 0.19364917 1.11935935 17.3555555555556

13.4666667 0.29580399 2.19656428 13.5666666666667

 Calculo de la potencia de la muestra en la ecuación obtenida
𝑦=𝑚∗log⁡(𝐶)+𝑏
𝑦=8,152∗log⁡(𝐶)+6,8031
𝐿𝑜𝑔(𝐶)=(𝑦−𝑏)/𝑚
𝐿𝑜𝑔(𝐶)=(13,57−6,8031)/8,152
10^(log⁡(𝐶))=10^((13,57−6,8031)/8,152)
𝐶𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑐𝑖ó𝑛= 10^((13,57−6,8031)/8,152)=𝟔,𝟕𝟔

Potencia
𝑝𝑜𝑡𝑒𝑛𝑐𝑖𝑎=(𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑐𝑖ó𝑛 ℎ𝑎𝑙𝑙𝑎𝑑𝑎 ∗100)/(𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑐𝑖ó𝑛 𝑡𝑒𝑜𝑟𝑖𝑐𝑎 )
𝑝𝑜𝑡𝑒𝑛𝑐𝑖𝑎=(6,76∗100)/5=𝟏𝟑𝟓,𝟐%

1.25 1.3 1.35

La muestra no se encuentra dentro de las especificaciones de la USP,

la cual nos indica que debe estar entre 95% - 105% de gentamicina
sulfato, y el valor de potencia que obtuvimos para esta se encuetra
por encima de estos valores con una potencia de 135,2% de
gentamicina sulfato, por lo cual no cumple con las especificaciones
n obtenida