BGLB Broth
Directions
Suspend 40.0 g of the dehydrated medium in 1,000
mL of distilled water, mix well and heat to dissolve
the medium. Distribute the medium into middle size
of test tubes containing a fermentation tube or a
Durham's tube. Sterilize by autoclaving at 121 °C for
15 minutes, and cool down rapidly for use. Do not use
any tubes that contain bubbles in a fermentation tube
or a Durham's tube.
To inoculate the test sample of more than
10 mL, use double concentration medium.
Keep strictly a sterilization time, and ensure
a rapid cooling before use.
Slight color discrepancy by lot to lot may be
observed.
Remarks
The medium is used for testing the presence of
coliform in food and water, and is also used for the
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determination of lactose fermentative bacteria. When
gas was evolved after incubation, coliform test is
positive.
In case of the examination of water,
incubate in Lactose Broth first, and when
the production of gas is observed,
transplant the culture to BGLB Broth. For the
test of food and drinks, add the specimen
directly to the medium and observer the
production of gas.
The medium inhibits the growth of gram-positive
bacteria, while it permits the proliferation of E. coli.
Formula
produced by Nissui Pharmaceuticls CO., LTD