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GUIDE BOOK
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Volgograd, 2012
УДК
Compiled by: Klauchek S. V., Lifanova E. V., Khvastunova I. V., Kudrin R. A.,
Akhundova R. E., Doletsky A. N., Schmidt S. A.
Approved by the Central Methodology Board of the Volgograd State Medical Uni-
versity.
This manual summarizes the practical tasks of human physiology of excitable tissues.
It caters for teachers and students in the English-speaking medium of higher medical
educational institutions.
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CONTENT
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Practical class 1. Introduction to the physiology.
Practical works
1. Making of nerve-muscular preparation.
2. First and second experiments of Galvani.
3. Secondary tetanus.
Books recommended
1. Ganong W. F. Review of Medical Physiology. 20th ed; McGraw-Hill Companies, Inc.,
2001. – P. 1-17, 27-35.
2. Guyton A. C., Hall J. E. Textbook of Medical Physiology, 12th ed; WB Saunders, 2005. – P.
43-59.
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2. Raise the nerve by the remainder of the backbone and cut carefully the adjacent tissues
with the help of a pair of scissors.
3. Cut off the leg above the knee-joint.
4. The remainder of the femur muscles should be also removed.
5. After that place the scissors under the Achilles tendon, separate it lengthwise and cut
below the sesamoid bone.
6. Cut off the leg below the knee-joint.
7. The nerve-muscular preparation of the gastrocnemius muscle and the sciatic nerve is
ready.
8. Wrap the preparation into the napkin moistened with Ringer solution and put it into Petri
dish.
Result:
Conclusion:
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Practical work 2. The first and second experiments of Galvani.
Result:
Conclusion:
Technique. II) The second experiment of Galvani (contraction which is not stimulated by
metal exposure).
1. Make a preparation of a frog’s rheoscopic leg and a damaged muscle of a nerve-muscluar
preparation.
2. Apply the nerve of the rheoscopic leg quickly to the damaged area of the muscle so that it
could touch simultaneously the damaged and the intact surface of the muscle.
3. Observe the contraction of leg muscles.
4. The experiment is successful, if the nerve is highly excitable and the femur muscle has
just been incised.
Result:
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Conclusion:
Objective: observing the arising currents of action in excited tissues and their possible
transmission to other tissues.
Technique.
1. Make a preparation of a frog’s rheoscopic hind legs and fix it in a support.
2. Place the nerve of the second preparation along the gastrocnemius muscle of the first hind
leg.
3. Using electrodes of a stimulator, send rhythmic stimuli to the nerve of the first
preparation to cause tetanic muscle contraction.
4.Observe muscle contraction of the second rheoscopic leg.
Result:
Conclusion:
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Practical works
1. Importance of physiological integrity of the nerve for conducting excitation.
2. Isolated conduction of excitation along the nerve fiber.
3. Bilateral conduction of excitation.
Books recommended
1. Ganong W. F. Review of Medical Physiology. 20th ed; McGraw-Hill Companies, Inc.,
2001. – P. 49-61.
2. Guyton, A. C., Hall, J. E. Textbook of Medical Physiology, 12th ed; WB Saunders, 2005. –
P. 60-70.
Objective: proving the importance of physiological integrity of the nerve for conducting
excitation in the course of the experiment.
Technique. A frog`s rheoscopic leg should be used for the experiment (see practical
work1).
1. Fix the bone of a frog’s rheoscopic leg in a support.
2. Apply the sciatic nerve onto the electrodes.
3. After that you should apply the ligature (or ammonium chloride, novocaine) onto the
nerve between the muscle and the electrodes.
4. Stimulate the nerve:
between the ligature and the end of the nerve;
between the muscle and the applied ligature.
Results:
Conclusion:
Objective: proving experimentally that excitation is conducted isolatedly within one fiber.
Technique. A frog’s hind leg preparation should be used for the experiment.
1. Place a thread under each nerve root.
2. Stimulate each root which spreads from the spinal cord by means of sending weak
rhythmic impulses (using an electrostimulator).
Mind that when different roots are stimulated, different groups of muscular fibers contract,
despite the fact, that the nerve fibers of these roots pass through the trunk of the sciatic nerve.
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Explain under what conditions isolation of excitation conduction along the nerve fiber
occurs?
Results:
Conclusion:
Objective: proving experimentally that excitation is conducted along the nerve fiber in both
directions from the stimulated area.
Technique. 1. Cut off gracilis muscle paying special attention to the branches of the nerve
on the inner surface of the muscle.
2. Disintegrate the muscle carefully into two parts so that the halves should be connected
with each other by means of nerve branches.
3. Stimulate one half of the muscle by means of sending rhythmic impulses with the help of
a stimulator.
4. Note that when stimulated, both halves of the muscle preparation should contract.
5. If you apply the electrodes onto the second half of the muscle, you’ll observe contraction
of both parts.
Draw a scheme of the experiment. In conclusion work out the law of bilateral conduction of
excitation.
Results:
Conclusion:
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7. Types of muscular fibers (red muscles and white muscles). Relation of muscle length and
tension to the velocity of muscle contraction.
8. Work and strength of skeletal muscles. Body mechanics.
9. Muscular fatigue. Muscular fatigue theory.
Practical works
1. Recording different types of skeletal muscle contraction.
Books recommended
1. Ganong W. F. Review of Medical Physiology. 20th ed; McGraw-Hill Companies, Inc.,
2001. – P. 62-80.
2. Guyton, A. C., Hall, J. E. Textbook of Medical Physiology, 12th ed; WB Saunders, 2005. –
P. 71-90.
Record the curves of single muscle contraction, incomplete and complete tetanus in the
protocol. Explain the mechanisms of the development of different types of skeletal muscle
contraction.
Results:
Conclusion:
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Practical class 4. Synaptic transmission.
Practical works
1. Work of the muscle. Dependence of work value on load.
2. Dynamometry.
Books recommended
1. Ganong W. F. Review of Medical Physiology. 20th ed; McGraw-Hill Companies, Inc.,
2001. – P. 81-114.
2. Guyton, A. C., Hall, J. E. Textbook of Medical Physiology, 12th ed; WB Saunders, 2005. –
P. 546-558.
A = P x h, where
1. Set a unit for recording muscle contraction. A neuro-muscular preparation is required for
the experiment. Fix the muscle in a myograph so that the forceps should be located above the
recorder. Connect the lower end of the muscle with the Achilles tendon at Engelman's level with the
help of a hook.
2. Apply various loads onto the same shoulder of the preparation (10, 20, 50, and 100 g),
stimulating the muscle by using an electrostimulator every time.
3. Record the frequency of muscle contractions on the drum of the kymograph every time it
has been stopped.
Calculate the work performed by the muscle and draw a curve of dependence of work value
on load values; mark load values on the axis of abscises and on the axis of ordinates (g/cm). In
conclusion formulate the rule of average loads.
Results:
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Conclusion:
Objective: measuring the maximal value of muscular effort and muscle endurance of the
hand.
Technique. 1. A student should stand up and draw aside one of his (her) arm with a
dynamometer in his (her) hand so that there is a direct angle between the arm and the trunk. The
second free arm is down and relaxed.
2. The student should press the dynamometer with maximum strength twice after a signal.
The best result is obtained to estimate muscle strength.
3. Then the student should press the dynamometer 10 times with the frequency of 1 time per
5 seconds consecutively.
4. Record the results and determine muscle efficiency according to the following formula:
5. These results are used for determining the index of decreased muscle efficiency according
to the following formula:
Calculate and record the power and the level of muscle efficiency in the protocol. Draw a
curve of decreased muscle efficiency.
Results:
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Conclusion:
Practical class 5. Concluding class devoted to the themes “Introduction to the physiology”,
“Excitable tissues: nerve tissue”, “Excitable tissues: muscle tissue”, “Synaptic transmission”
(intermediate oral examination).
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