EXERCISE NO.
 Directions: Make an outline of the steps in dispensing the following media. (3 pts each)
  Nutrient Agar Plate
.
Fill the plates with around 10-15
mL of agar. The plate's foot
should be secured. Twirl the
plate somewhat on the off
chance that required to equally
disseminate the agar. Permit the
dishes to cool overnight on the
counter to maintain a strategic
distance from condensation. To
keep the agar from drying out,
put it upside-down in plastic
sacks within the fridge.
CULTURE PREPARATION
     Nutrient Broth
Use autoclave tape to label
the beaker, starting what  the beaker, starting what
media is being prepared, the
date and your initials (i.e
Nutrient broth 8-18-08 KH).
Fill the beaker halfway with
powder then halfway with the
required amount of water. To
combine, use a glass string
rod. Heat in the microwave
for 3-5 minutes at a time.
Nutrient Agar Slant and
Nutrient Agar Butt
Use autoclave tape to label
 
media is being prepared, the
date and your initials (i.e
Nutrient broth 8-18-08 KH).
Fill the beaker halfway with
powder then halfway with the
required amount of water. To
combine, use a glass string
rod. Heat in the microwave
for 3-5 minutes at a time.
Results and Discussion: (3 pts each)

1. What are the precautionary measures that must be observed to ensure that the
culture media are free from contamination?  (Answer the following in 4 to 5 sentences)

To preserve an aseptic work environment , everything you work with ought to be at first free of
organisms . Hence , we start with pre-sterilized pipettes , culture tubes , and dish sets .
Immunizing circles and needles made of metal wire can be utilized to exchange microbes from
one medium to another , such as from the surface of an agar plate to a broth . Metal
apparatuses may be sterilized by warming them within the fire of a Bunsen burner . Glass
apparatuses or metal spreaders or forceps that ca n't be sterilized by coordinate warm are
plunged in liquor taken after by a brief pass through the fire to speed the dissipation prepare .

2.   How can it be determined if the prepared medium is not contaminated?

In case a few of the agar plates gotten to be sullied, one can frequently tell by looking at the
plate how defilement took put. In the event that the contaminants are imbedded within the agar,
the contaminant was likely poured with the medium. In the event that the defilement is
underneath the agar it could have been within the dish some time recently itwas poured or
entered when the cover was being opened to pour. In case the defilement is on beat it may
have come in some time recently the cover was closed after pouring, whereas opening the
cover to wipe out condensation or whereas the plate was being vaccinated
  

3.    Enumerate the different classifications of culture media according to physical

state, composition, use or purpose.

● Basal Media - this media supports most non - fastidious bacteria . Peptone water , nutrient
broth and nutrient agar are considered as basal medium
. ● Enriched Media - which are used to nutritionally exacting bacteria . Adds nutrients in the form
of blood , serum , egg yolk to basal medium , it makes them as enriched media .
 ● Blood Agar - prepared by adding 5 - 10 % to a basal medium , such as nutrient agar or blood
agar bases . Blood agar is useful in demonstrating hemolytic properties of certain bacteria .
● Heated Blood Agar or Lysed Blood Agar - blood is added while the molten blood agar base is
still hot . This lyses the blood cells and releases their properties on the media . In this process ,
it turns the media into a medium brown
● Selective Media - used to inhibit unwanted growth of microorganisms and helps recover
pathogens from a mixture of bacteria . Selective media are agar based , enrichment media are
liquid in consistency
. ● Differential Media - it distinguishes one microorganism from another growing on the same
media . Uses the biochemical characteristic of a microorganism growing in the presence of
specific nutrients , then added to the medium to indicate the variation of characteristics of
microorganisms
. ● Transport Media - all specimens must be transported to the laboratory immediately after
collection to prevent overgrowth of contaminating bacteria and this is attained by using transport
media .
 ● Anaerobic Media - it reduces the potential and extra nutrients .

4. Cite examples of different types of culture media

  Basal Media- basal media are those media that are used for growth of bacteria
that do not need enrichment media.

 Enriched Media- media are enriched with adding blood, serum, or egg yolk.
 Selective Media- media that supports the growth of specific bacteria by inhibiting
the growth of undesired bacteria and allows growth of desirable bacteria.
 Indicator / Differential Media- a particular organism causes changes in the
            inhibitor. Blood agar and MacConkey agar are examples of indicator media.
 Transport Media- these media are used when a specimen is not cultured soon
             after collection.
 Storage Media- media used for storing or housing the bacteria for a long period
            of time.

5.   What are the factors to consider when preparing culture media?
 Before one can develop a medium that will accomplish a craved result within the development
of living beings, one must get it their basic needs. Any medium that's to be reasonable for a
particular gather of life forms must take into accountthe taking after seven components: water,
carbon, vitality, nitrogen, minerals, development variables, and pH.

6.  What are the proper ways of storing culture media if they are not in use?

The prescribed shelf-life of arranged culture media shifts significantly. Screw-capped bottles of
supplement broth and agar can be put away for 6 months at moo surrounding temperatures (12-
l6°C). It is critical to store all media absent from light. Agar plates ought to be put away at 2-8°C
in fixed holders to maintain a strategic distance from misfortune of dampness. Don't Solidify.

7.   Enumerate the importance of culture media in cultivating microbes

 To separate microbes in immaculate cultures

 To illustrate microbial properties
 To get adequate development of microscopic organisms for the arrangement of antigens
and for other tests
  To decide affectability of microorganisms to anti-microbials