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Relationship Between Body Condition Scores and Conception

at First Artificial Insemination in a Large Dairy Herd


of High Yielding Holstein Cows1
J. J. DOMECQ,2 A. L. SKIDMORE,2 J. W. LLOYD,3
and J. B. KANEENE3
Michigan State University, East Lansing 48824

ABSTRACT INTRODUCTION
This study investigated the relationship between After parturition, dairy cows experience a slow in-
changes in body condition during the dry period and crease in DMI, a rapid increase in milk yield, and
early lactation and conception to first postpartum AI. increased mobilization of body adipose (1, 15, 16, 25,
Holstein cows ( n = 720) on a commercial dairy farm 32). An estimated 80% of cows experience negative
were scored weekly for body condition beginning at energy balance in early lactation because the energy
dry-off and continuing until first AI. Occurrence of demands for milk yield are not met by the diet (1, 15,
postpartum diseases was recorded. A multiple logistic 16, 25, 32). Individual cows respond to negative
regression model was a significant predictor of the energy balance through different combinations of feed
success or failure of conception for multiparous cows, intake, mobilization of body adipose, and lower milk
but not for primiparous cows. Principal component yields (1, 15, 25). The reproductive performance of
analysis reduced collinearity among independent cows, particularly the probability of conception, may
be associated negatively with the magnitude and
variables and allowed the variables to be ranked
severity of negative energy balance in early lactation
based on their contribution to the interval from first
(1, 25).
AI to conception. The top three ranking variables
Body condition score ( BCS) is a management tool
were lactation number, milk yield at 120 d of lacta-
that is used to monitor the amount and mobilization
tion, and change in body condition score between
of body adipose and thus indicates a negative energy
parturition and wk 4 of lactation. Increased milk balance of cows in early lactation (1, 8, 15, 16, 25). If
yield at 120 d of lactation was associated with an conception is related to BCS, magnitude and changes
increased likelihood of conception, and decreased body in BCS during early lactation could be used to deter-
condition during the 1st mo of lactation was as- mine whether nutritional goals are achieved and to
sociated with a decreased likelihood of conception. identify potential problems (1, 15, 25).
Health problems were less associated with conception The BCS of a cow at parturition appears to have no
than were body condition or milk yield in this herd. relationship with conception or AI services per con-
Body condition during the dry period and during the ception (1, 25, 27, 29, 30). Garnsworthy ( 1 5 ) con-
first 30 d of lactation is an important tool to identify cluded that the BCS at parturition or BCS at AI was
cows at risk for failure to conceive at first AI. not related to conception. However, the loss of BCS
( Key words: body condition score, conception) between parturition and AI may negatively influence
conception. Butler and Smith ( 1 ) , Ferguson (11),
Abbreviation key: BCS = body condition score, CFS
and Ferguson and Otto ( 1 3 ) reported similar results
= conception at first AI service, DBCS = BCS at dry- of conception to first AI services ( CFS) for cows
off, MYA = milk yield acceleration, PCA = principal grouped according to losses of BCS. Cows that lost 0.5
component analysis. to 1.0 points of BCS had a 53% rate of CFS, and cows
that lost >1.0 points of BCS between parturition and
AI had a rate of 17%. Ruegg and Milton ( 3 0 ) identi-
fied a marginal association ( P = 0.09) between BCS
Received October 26, 1995.
Accepted May 3, 1996. loss and number of AI in a multiherd trial. In another
1This research was conducted as part of Regional Research study, Ruegg et al. ( 2 9 ) indicated that loss of BCS
Project NC-119, Dairy Herd Management Strategies for Improved was not related to AI per conception.
Decision Making and Profitability.
2Department of Animal Science. Several of the previously mentioned studies used
3Population Medicine Center. AI per conception to investigate the relationship be-

1997 J Dairy Sci 80:113–120 113


114 DOMECQ ET AL.

tween BCS and fertility of cows. The number of AI per BCS


conception may not be the best indicator of the role of
BCS in the success or failure of conception, because All BCS were assigned by one individual using the
the magnitude and duration of negative energy visual technique developed by Edmonson et al. ( 8 ) .
balance, as monitored by BCS, changes during lacta- Cows were scored to the nearest quarter point. The
tion. A more direct method to evaluate the relation- BCS in this study were validated with ultrasound
ship between BCS and conception was utilized by measurements of subcutaneous fat ( 5 ) . All cows were
Butler and Smith ( 1 ) , Ferguson (11), and Ferguson dried off on Thursdays, and the BCS at dry-off
and Otto (13); only CFS was considered in those ( DBCS) was obtained the next day. The BCS were
studies. assigned weekly throughout the dry period, and the
Typically, as cows move through lactation, the scorer had no knowledge of the previous BCS. Once a
magnitude and duration of negative energy balance cow entered the study, a BCS was assigned every
decreases. If negative energy balance, as monitored week. The BCS at parturition was determined by
by changes in BCS, is associated with CFS, the as- using the BCS closest to parturition. The BCS at first
sociation would probably be in early lactation when postpartum AI was determined by using the weekly
energy balance is most negative. Use of BCS to moni- BCS closest to the AI date.
tor changes in negative energy balance during the dry Nulliparous cows entered the study when available
period and during the first 30 d of lactation (peripar- for prepartum body condition scoring, generally 2 to 3
tum) may be important to CFS. wk before expected parturition. A BCS was assigned
The association between peripartum BCS and CFS weekly during this prepartum period, and the BCS at
has not been examined in high yielding (>11,000 kg/ parturition was determined in the same manner as
yr) cows that may have greater risk of negative described for lactating cows. After parturition,
energy balance and decreased BCS (25). Some of the primiparous and multiparous cows were assigned a
previous studies did not control for biological and BCS during weekly herd visits.
management factors, such as season, milk yield, and
health disorders, which may also influence conception Milk Yield
and bias results.
The goal of this research was to investigate the The daily milk yield for each cow was recorded
relationship between BCS and CFS in a herd of high electronically and stored via computer on the farm.
yielding Holstein dairy cows. The research hypothesis The first 120 d of lactation were divided into seven
tested was that BCS or changes in BCS during the periods to characterize rates of milk yield and to
dry period or early lactation were associated with calculate milk yield acceleration ( MYA) and total
reduced probability of CFS. The specific objective was milk yield. The MYA represents the rate of milk yield
to determine whether BCS or changes in BCS during for each period and may more accurately reflect bio-
the dry period or early lactation were associated with logical changes experienced by the cow during early
a reduced likelihood of CFS when management risk lactation. Calculation of MYA has been described
factors and postpartum health disorders were con- previously (6, 7). Associations between MYA and
trolled. CFS have been identified ( 7 ) . There were six
15-d periods beginning at parturition, and the
MATERIALS AND METHODS seventh period represented 91 to 120 d of lactation.
For each period, days of lactation were regressed
on daily milk yield. The regression coefficient ob-
Cow Selection
tained in the analysis was referred to as the MYA and
A commercial dairy farm (1000 cows) located in represented the rate of change in milk yield for the
south central Michigan was used. Facilities and herd respective period. The regression coefficients (MYA)
management have been described previously ( 6 ) . were then used to estimate milk yield for each day in
Multiparous cows entered the study at dry-off. All the period. The estimated daily milk yields were then
cows that were dried off during 1993 were included in combined to obtain total yield for each period. This
the study. All heifers that calved for the first time procedure compensated for missing daily milk yields
between February 15, 1993 and February 15, 1994 because of missing or malfunctioning electronic trans-
entered the study prior to first parturition. Data col- ponders, computer failures, or cows that were not
lection terminated on all cows at 120 d of lactation or milked or were missed on a given day. Total yield for
at first AI if cows were at >120 d of lactation. For each period was added to obtain total milk yield at
culled cows or cows that died before 120 d of lactation, 120 d of lactation. A cow must have had at least five
no data were analyzed. daily milk weights in a period for MYA to be calcu-

Journal of Dairy Science Vol. 80, No. 1, 1997


BODY CONDITION AND CONCEPTION 115

TABLE 1. Definitions of health disorders. where Y = probability of CFS, e = base of natural log,
Disorder Definition and f(BCS) = intercept + DBCS + DDBCS + DPBCS +
Dystocia Assisted delivery
LAC + TMY + MYA + days to first AI + winter +
Retained placenta Retention of fetal membranes >12 h spring + summer + DD + dystocia + twins + RP + MET
Metritis Cloudy discharge and enlarged uterus + DA + MF + lameness + error where DDBCS =
during rectal exam change in BCS during the dry period; DPBCS =
Lameness Any abnormality in locomotion
Displaced abomasum Pinging sound upon abdominal ausculta- change in BCS from parturition to wk 4 of lactation;
tion and surgical correction LAC = lactation number; TMY = total milk yield at
Milk fever Weakness, cold skin, and favorable 120 d of lactation; MYA = MYA between 1 and 15 d of
response to calcium therapy
lactation; winter, spring, and summer = season of first
AI; DD = days dry; RP = retained placenta; MET =
metritis; DA = displaced abomasum, and MF = milk
lated and must have had MYA in each of the seven
fever.
periods, or the cow was omitted from the analysis.
All health disorders were considered as discrete
CFS variables with binary outcomes (no = 0; yes = 1).
Because heifers were not scored for DBCS before par-
If a cow was confirmed pregnant 35 to 40 d after turition, primiparous cows were analyzed separately
first AI, CFS was achieved. If a cow received a second with a similar model that did not include DBCS,
AI, CFS did not occur. A successful CFS was coded 1, DDBCS, DD, and LAC variables. The BCS at parturi-
and an unsuccessful CFS was coded 0. Cows identi- tion was included in the model for first lactation cows.
fied by herd personnel as not being eligible for breed- Change in BCS during the dry period was deter-
ing and, therefore, not inseminated were omitted mined by subtracting the BCS at parturition from
from the analysis. The number of days to first AI also DBCS; a negative number indicated an increase in
was recorded. BCS from dry-off to parturition. The change in BCS
during the 1st mo of lactation was calculated by
Health Disorders subtracting the BCS at wk 4 of lactation from the
BCS at parturition. The change in BCS between par-
All health disorders were diagnosed and recorded
turition and first AI was determined by subtracting
by herd personnel or by the herd veterinarian. The
the BCS obtained nearest to first AI from the BCS at
data were collected during weekly visits to the farm.
parturition.
Each health disorder was coded as occurring or not
Season of first postpartum AI was considered to be
occurring (no = 0; yes = 1). Repeat occurrences or
recordings of the same health problem in the same discrete and was previously described ( 6 ) . By its
cow were not included. The disorders of interest were omission from the model, fall was the basis for com-
dystocia, twins, retained placenta, metritis, milk parison of seasonal effects.
fever, displaced abomasum, and lameness. Definitions
of health disorders are shown in Table 1 and are Odds Ratios
similar to those used in previous studies (2, 3, 9, 16,
Odds ratios are a measure of the strength of associ-
17).
ation between independent and dependent variables
and are used extensively in epidemiological studies
Statistical Analysis (10, 16, 21, 28). Odds ratios are the natural log of the
A multiple logistic regression model was developed parameter estimate obtained in the logistic regression
to test the hypothesis that BCS or changes in BCS analysis. An odds ratio of 1.0 suggests no association
during the dry period or early lactation were as- between the independent and dependent variables.
sociated with reduced probability of CFS. Logistic Standardized odds ratios were calculated for the con-
regression was used because the dependent variable, tinuous independent variables. Standardization of
CFS, was a discrete variable with only two outcomes. odds ratios for continuous variables allowed for the
To demonstrate accurately the influence of BCS and expression of odds ratios for one standard deviation of
changes in BCS on CFS, specific management and change in the continuous variable.
health disorders known to influence CFS were con-
trolled in the analysis. The statistical model that was Principal Component Analysis
used is shown in Equation [1]:
When a correlation exists between any set of in-
1 dependent variables, the true contribution of each
Y =
1 + e–f(BCS) [1] variable to the outcome of interest may not be demon-
Journal of Dairy Science Vol. 80, No. 1, 1997
116 DOMECQ ET AL.

TABLE 2. Incidence of health disorders in multiparous cows. in this study, a logistic multiple regression model was
Health disorder Incidence developed because of the discrete dependent variable.
(%)
Metritis 19.9 RESULTS
Retained placenta 8.7
Lameness 2.8 A total of 720 cows was included in the analysis.
Milk fever 1.4 The rate of CFS for 426 multiparous cows was 46%,
Displaced abomasum 0.5
Twins 2.8 and the rate of CFS for 294 primiparous cows was
Dystocia 6.5 47%. Mean annual milk yield was >10,500 kg per cow.
Milk yield, MYA, and BCS of this herd during the
current study have been previously reported ( 6 ) . In-
cidence of health disorders for multiparous cows com-
strated. To avoid this situation, Pearson correlation pleting the study is presented in Table 2. Metritis had
coefficients were calculated among the independent the highest incidence in this herd; 19.9% of mul-
variables of the regression model to detect the tiparous cows in this study were diagnosed with
problem of collinearity. Because of the high collinear- metritis.
ity found among the independent variables in the The results of the logistic regression analysis for
regression model, principal component analysis multiparous cows are shown in Table 3. The logistic
( PCA) (20, 22, 23, 26) was used to reduce collinear- regression model was a significant predictor of CFS
ity, to quantify the association of each independent for multiparous cows ( x2 = 34.241; P = 0.012).
variable with the probability of CFS, and to rank the Although the model was significant, only one in-
independent variables in the model in order of contri- dependent variable was associated significantly with
bution to the variance of CFS. CFS. In this herd, a cow that received first post-
Principal component analysis has been used by partum AI during summer was three time more likely
several researchers in veterinary and animal science to conceive to first AI than were cows that were in-
(12, 22, 26). The PCA techniques used in this study seminated during fall ( P = 0.003). The 95% confi-
have been described elsewhere (6, 20, 23). However, dence interval for the odds ratios was 1.4 to 6.2. The

TABLE 3. Parameter estimates, odds ratios, and 95% confidence intervals from logistic regression
analysis of multiparous cows.
Odds ratio1
Parameter
Variable2 estimate Discrete Continuous 95% CI
DBCS 0.03 0.93 (0.73, 1.4)
DDBCS 0.06 1.01 (0.71, 1.4)
DPBCS –0.37 0.86 (0.62, 1.2)
Lactation number 0.14 1.18 (0.94, 1.4)
Milk yield at 120 d of lactation 0.0002 1.14 (0.91, 1.4)
MYA 0.10 1.08 (0.86, 1.3)
Days to first AI –0.006 0.87 (0.70, 1.1)
Days dry 0.008 1.10 (0.89, 1.3)
Retained placenta 0.12 1.13 (0.50, 2.5)
Milk fever 0.21 1.24 (0.20, 7.3)
Lameness 0.70 2.01 (0.49, 8.2)
Metritis 0.38 1.46 (0.85, 2.5)
Displaced abomasum –0.11 0.89 (0.05, 3.7)
Twins –1.01 0.36 (0.09, 1.4)
Dystocia 0.21 1.23 (0.53, 2.8)
Season
Winter 0.18 1.19 (0.70, 2.0)
Spring 0.53 1.70 (0.93, 3.1)
Summer 1.1 3.02 (1.4, 6.2)
1Odds ratios for continuous independent variables were calculated for one standard deviation of
change.
2DBCS = Body condition score (BCS) at dry-off; DDBCS = change in BCS during the dry period;
DPBCS = change in BCS during the 1st mo of lactation; MYA = milk yield acceleration during the first
15 d of lactation; and winter, spring, and summer = season of first postpartum AI.

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BODY CONDITION AND CONCEPTION 117

TABLE 4. Parameter estimates, standard deviations, odds ratios, and rank of independent variables
obtained after principal component analysis of multiparous cows.
Odds Ratio1
Parameter
Variable2 estimate SD Discrete Continuous Rank
Lactation number 0.18 1.15 1.22 1
Milk yield at 120 d of lactation 0.0002 654.4 1.13 2
DPBCS –0.39 0.40 0.86 3
Days dry 0.01 12.37 1.13 4
Days to first AI –0.005 22.4 0.89 5
MYA 0.10 0.80 1.08 6
DBCS –0.14 0.51 0.93 7
DDBCS 0.03 0.48 1.01 8
Summer 0.55 0.018 1.73 9
Winter 0.32 0.02 0.72 10
Metritis 0.37 0.02 1.45 11
Lameness 0.34 0.008 1.40 12
Retained placenta 0.15 0.014 1.16 13
Twins –0.19 0.008 0.83 14
Milk fever –0.17 0.006 0.84 15
Dystocia 0.08 0.01 1.09 16
Spring 0.03 0.02 1.03 17
Displaced abomasum –0.14 0.003 0.87 18
1Odds ratios for continuous independent variables were calculated for one standard deviation of
change.
2DBCS = Body condition score (BCS) at dry-off; DDBCS = change in BCS during the dry period;
DPBCS = change in BCS during the 1st mo of lactation; MYA = milk yield acceleration during the first
15 d of lactation; and winter, spring, and summer = season of first postpartum AI.

95% confidence interval for the odds ratios suggested Cows that lost one standard deviation or 0.40 points
no significant relationship between the dependent of BCS during the 1st mo of lactation were 1.17 (1/
and independent variables, because 1.0 was included odds ratio) times less likely to conceive than were
in each 95% confidence interval. Based on the results cows that did not lose points of BCS during the 1st mo
of the logistic regression analysis, BCS or changes in of lactation. Cows that lost two standard deviations or
BCS during the dry period or early lactation were not 0.80 points of BCS during the 1st mo of lactation were
associated with CFS. 1.36 times less likely to conceive than were cows that
For primiparous cows, a similar logistic regression did not lose points of BCS. The DBCS was ranked
model was not a significant predictor of CFS ( x2 = seventh, and gaining BCS during the dry period was
13.81; P = 0.4340). No further analysis was per- ranked eighth; lower DBCS and an increase in BCS
formed for primiparous cows. There was no relation- during the dry period increased the likelihood of CFS.
ship between BCS or any other variable included in Health disorders ranked lower than did the measures
the model and CFS.
of BCS, suggesting that they have less association
Pearson correlation coefficients among the in-
with CFS than did BCS.
dependent variables indicated a high degree of associ-
ation or collinearity. Correlations were as high as
0.54, indicating the need for PCA to reduce collinear- DISCUSSION
ity of the independent variables in the logistic regres-
The change in BCS during the 1st mo of lactation
sion model for multiparous cows.
Results of the logistic regression after PCA for was included in the logistic regression model because
multiparous cows are presented in Table 4. Ranking the correlation between change in BCS during the 1st
indicated the strength of the association of indepen- mo of lactation and change in BCS between parturi-
dent variables in the regression model with CFS after tion and first postpartum AI was 0.72 ( P < 0.05).
reducing collinearity. The association between lacta- Negative energy balance is most severe during the
tion number and CFS was ranked first, followed by first 2 wk of lactation and may influence physiological
total milk yield at 120 d of lactation, and change in functions during this time that are important to con-
BCS during the 1st mo of lactation. ception (1, 25). The likelihood of CFS may be deter-
The change of BCS during the 1st mo of lactation mined by the loss of BCS during the 1st mo of lacta-
was ranked third among the independent variables. tion.
Journal of Dairy Science Vol. 80, No. 1, 1997
118 DOMECQ ET AL.

Hillers et al. ( 1 9 ) reported CFS rates of 54% for cows in this study. Overconditioning during the dry
primiparous cows and 50% for multiparous cows. The period has been related to health and reproductive
rates of CFS for primiparous (47%) and multiparous problems (14, 24, 32), but the cows in this herd were
cows (46%) in the current study were similar. not overconditioned (DBCS = 2.77). Cows in this
Gearhart et al. ( 1 6 ) reported a 44% CFS rate for herd could gain condition during the dry period
cows in a multiherd study. Based on the recommenda- without becoming overconditioned.
tions developed by Domecq et al. ( 4 ) , the conception Higher yielding cows in this herd were more likely
rate observed in this herd would be considered to be to conceive than were lower yielding cows. There may
only a slight problem. be an antagonistic relationship between milk yield
Body condition score and CFS in primiparous cows and reproductive performance (1, 15, 25). However,
in this herd were not associated. Primiparous cows in Shanks et al. ( 3 1 ) suggested that high yielding cows
this herd might not have experienced the magnitude might be more reproductively sound because they are
or duration of negative energy balance in early lacta- healthier than are lower yielding cows. The measures
tion, as monitored by BCS, to reduce the probability of BCS in the logistic regression model in this study
of CFS. might have accounted for the effects of negative
The logistic regression model for multiparous cows energy balance that influenced conception and re-
was a significant predictor of the probability of con- moved the negative association of milk yield and
ception. However, only one variable was significantly reproductive performance.
associated with CFS. Cows inseminated in summer After collinearity was reduced with PCA, the as-
were 3.02 times more likely to conceive to first AI sociation between summer AI and CFS became
than were cows inseminated during fall. However, weaker, suggesting that other factors, including BCS,
because of the collinearity among the independent were more important to CFS. Conception rates in
variables, the results of standard logistic regression summer were generally lower than those in other
might not be accurate. The relationship between an seasons of the year (18). The summer season might
independent variable and CFS may be masked or not have been warm enough to have an influence on
inflated because of the effects of collinearity. More CFS in this herd. Any possible negative association
reliable associations can be established with PCA. between summer AI and conception might be seen
The PCA for multiparous cows indicated that the through changes in BCS and other management fac-
three measures of body condition were ranked higher tors and might not be as important to CFS as previ-
than health disorders and had a greater association ously thought.
with CFS in multiparous cows. Cows that lost one The incidence of most health disorders in this herd
point of BCS in the 1st mo of lactation were 1.5 times was similar to and within ranges of incidences
less likely to conceive than were cows that did not reported in other studies (9, 16, 17). However, the
lose one point of BCS. In other words, if the CFS for a 20% metritis rate might have been inflated because of
group of cows that did not lose one point of BCS was the aggressive diagnosis and treatment of uterine
60%, cows that did lose one point of BCS would have infections by herd personnel.
a conception rate of 45%. The loss of BCS for cows in Health disorders were ranked last among the in-
this herd did not influence CFS as much as has been dependent variables. The lack of association between
reported previously. Ferguson ( 1 1 ) and Ferguson and health disorders and CFS might have been due to
Otto ( 1 3 ) reported that a cow that lost one point of several factors. The degree, or severity, of specific
BCS between parturition and first AI was 2.0 times health disorders was not accounted for in this study
less likely to conceive at first AI. No relationship was and might have had a stronger association with CFS.
found between the number of AI per conception and Repeat occurrences of health disorders were not ac-
the change in BCS between parturition and first AI in counted for, which also might have negatively in-
several studies (29, 30, 33). Although AI per concep- fluenced CFS. Health disorders might have had a
tion were related to CFS, the relationship between greater influence on CFS in herds in which preven-
BCS and CFS was not addressed in previous studies. tion and treatment were not considered as important
By controlling for management risk factors and as they were in this herd.
health disorders and by using PCA, the odds ratios The lack of a strong, direct association between
obtained in the current study might estimate more health and CFS in this herd does not exclude the
accurately the importance of BCS to conception. possibility of an indirect influence of health on BCS
Decreased BCS at dry-off and increased BCS dur- and changes in BCS. Using PCA minimized associa-
ing the dry period increased the likelihood of CFS for tions among independent variables, such as health

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BODY CONDITION AND CONCEPTION 119

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CONCLUSIONS England.
16 Gearhart, M. A., C. R. Curtis, H. N. Erb, R. D. Smith, C. J.
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Environmental and managemental factors affecting conception
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more important to CFS than were health disorders or ing. 1984. Effects of production, season, age of cow, days dry,
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21 Kleinbaum, D. G., L. L. Kupper, and K. E. Muller. 1988. Ap-
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22 Lafi, S. 1989. Epidemiologic and economic study of repeat 29 Ruegg, P. L., W. J. Goodger, C. A. Holmberg, L. D. Weaver, and
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27 Pedron, O., F. Cheli, E. Senatore, D. Baroli, and R. Rizzi. 1993. 32 Van Saun, R. J. 1991. Dry cow nutrition: the key to improving
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