tne proper way of washing red cll. the purpose of washing the red cells, |. Harmening, D. (2019), Modem Blood Banking & Transfusion Practices. rectly % ee Philadelphia, PA: FA. Davis Compary. ion in flerent tests performed in blood benk. ‘A frer ratio of plasma toyed cel is important for accuracy in antigen-antibody ‘concentatoy of els mght feu no weak ofoee noone ton albumin:globulin ratios ‘$0 In the preparation of the cell suspension. RBCs can be obtained by ‘clotted (red top) specimens. This method is particularly useful for = of cells necessary for adsorption and elution techniques, and is needed ygemagglutination tests. The cells ak cell suspensions are used gglutination tests since the ratio of serum to cells affects the sensitivity of most 4 minimum number of antibodies must bound to RBC'S in order to bring about ination, A. itive or false negative or serum, They ie strength specimens: blood specimens, provided in “serum separator’ tubes. Patient plasma/serum is separated from the red cells and placed into an ately labeled tube with the patient's name and medical record number or date of 32NTTLE: ABO & Rn FORWARD TYPING: SLIDE AG and ft nbratory act. he student shal be te Faforn the ABO Forward Tying Side Method ‘Your cassroom instructor for this subject, PREINTERNSHIP MODULE - BLOODBANK ‘is Hans ‘Dale R Rocemora, RMT. MANN LESSON ABOBLOOD GROUPING discord fy Kort Lapteteiter (70) ABO grouping fs performed so thet blood may be matched if a transfusion necessary. An individual should be transfused with blood ofthe same ABO group. to avoid siving the patient an antigen he does not already have. Tha’ABO remains the al in transfusion practice. Transfusion of an incorrect ABO ‘death of a patient. Forward grouping is defined as u nanan souress of reagent anisera(ent-A, ‘ant-8) to detect antigens on an individual's red cells. Reverse grouping is defined as using feagent cells with known A and B antigens and testing the serum ofthe patient for ABO ‘10up antibodies. ABO antibodies are general racteristically, IgM antibodies are fokt-reactng antibodies that bind complement cross the placenta, Serum from {1OUP O individuals contains not only anti-A and anti-B but also anti-AB. Anti-A, B from {r0up O individuals has been reported to be a mixture of IgG and IgM, or IgG, IgM and IgA. BB crosses the placenta more frequently than anti-A or anti B, confirming the ‘mostimgortant type can result in ‘The group © serum is included in the ABO forward typing procedures as itis more foweak A and B antigens (subgroups of A, B and AB) than are anti-A and 8 2, Serum af groups O individual contain in addition to anti-A and anti-B ‘an agglutinin that is caled anti-C, which is directed against a C factor shared ‘A and B. Ant-C is important for two reasons: the placental barrier more readily than naturally ocourring anti-A and Teto be antbody most often involved in ABO hemoytc diseases of OmeSorensen eas een aa (6 mL), test tube rack, droppers, applicator stick, ae eas ee eee ee Te so game mses am MN of he laboraiory activity, the student shall be leer patble blood Wranstsion reaction. poke foe reste wth the sonicance of Hamening, 0. (2019) Made Blood Coren, el 12 significance ra, BRENT ATON (omnes out classroom instructor for this subject, PREINTERNSHIP MODULE ~ BLOODBANK is Hans - To determine that blood given to the patient is safe and won't cause adverse reactions - Consists of a series of tests performed on the biood of the prospective recipient of a blood transfusion and on the blood of the proposed donor - To minimize the risk of blood transfusion to a patient by selecting blood and blood components that will have acceptable survival when transfused and will not cause destruction of the recipient's red cells To prevent a transfusion reaction whether itis hemolytic reaction or a less severe one Chapter 10, page 247 ‘do we need to do a crossmatch test? * Tohave a final check of ABO compatiblity between donor and patient * To detect the presence of an antibody in the patient's serum that will react with antigens on the donor cells but was not detected in the antibody screening because the corresponding antigen was lacking from the screening cells Uused to determine the compatibility between red cells of the donor and the recipient (DROPS) Ros rd Pind ely of pation! seamta [woent ACTIVITY SHEET? 'BS MEDICAL TECHNOLOGY / FOURTH YEAR Session # 9 for Activity 9 Materials: POCT [ess0N TITLE: POCT Inmunochromatographic Test for Gropper) Neon Tost Kk a References: OUTCOMES: ARM of the exerci, th student shall be expectedto; MePharon. R.A. Bian MR. & Hany, Ai derstand the principle behind the test aero 2. know the procedures involved in POCT HBsAg Pilelebhe Sandee le re 43. Perfor the procedures accordingly 1M Stevens, Christine. (2016) Clinical 4. Interpret the postive ar HBsAg FA. Davis Company ST Ae ee UBJECT ORIENTATION (20 minutes) 3 ‘Your classroom instructor for this subject, PREINTERNSHIP MODULE ~ SEROLOGY ~ POCT Immunochromatographic Test for HBsAg is Dolie Keith 8. Masbang, RMT. Listed below are the addtional information vital in orientation: Main Lesson << a Hepatitis B, also called “serum hepatitis’, is caused by the hepatitis 6 virus (HBV). Hepatitis B is ‘a complex DNA virus that Belongs to the family Hepadnaviridae; a member family is known as hepadnavirus. Viral proteins of importance include the following: 1. The envelope protein-H{ 2. structural nucleocapsid core protein-hepatitis B core Antigen (HBcAg) 3. A soluble nucleocapsid protein- hepatitis B e antigen (HBeAg) HBV spreads through infected body fluids, such as blood, saliva, semen, vaginal secretion, tears breast milk, sweat and urine. Mode of transmission includes: sharing of contaminated needles or syringes, blood transfusion or sexual intercourse with an HBV-infected person. HBV- infected mothers can also transmit the virus to their newborn babies. . The development of a rapid immunochromatographic testis an initial qualitative screening testfor the detection of HBV infection from patient's serum, plasma or whole blood specimens inthis est, the kit is incorporated with all the reagents in the absorbent membrane enclosed Ia plastic cassette, When sample is added, it migrates through the membrane reacting with the reagents and forming color band a Procedure * There are varying procedures per manufacturer when doing POCT Immunochromatographic Test for HBsAg. (Please see package insert for specific Procedure)Sean, Healt ope “Maia. Hed = MEA(Mamitor san spar) sere nD Wtivei OT cub utah Chovacteitics (Aypie “Plate cotenier ~cctony Sieg: Punetifor : 76 activity SHEET ‘PREINTERNSHIP MODULES. | [BS MEDICAL TECHNOLOGY / FOURTH YEAR et = PrrLe: OBTAINING PURE CULTURE: a 1G OUTCOMES: entre iat hl pussy oe 3 » ersiod te importance of streak plate method for * potent dexter for the steak plate method, rs the isolated colonies ater performing the + Serpe method = = a ‘Your classroom instructor for this subject, PRE-INTERNSHIP MODULE - MICROBIOLOGY jamie 8 Baran, RMT. OBTAINING PURE CULTURE Pure cultures are essential for microbiological studies. Obtaining pure culture of ‘cera is usually accomplished bacteria on the surface of a solid medium $0 that a single cel occupies an isolated portion of the agar surface. This single cell wil go through repeated. rutipication to produce a visible colony of similar cells, or clones. » a ‘Streak plate method js usually being used for isolation of mixed culture of bacterial ‘ose, Techniques for his method inciyge the simple, radial gnd muliplenterupted. Am: tte aforementioned techniques, it is the muttiple interrur iat iS consi "as the kine it enables the microbiologists to have isl eS. pyigte iterphed Ah rat dead Procedure: j A. First Meeting: PRIMARY PLATING ~ Streak Plate Method 1. Moisten a sterile cotton swab with sterle NSS and get a swab from the following: Groups Source oruomoa, Go Nocti " a 7 armpit 3 Palm 8 Ee 4 fan 9 AmFolds 5 Face D oy 2. Partially open the(A)plate with space just enough to insert the coton swab. Ral he cotton swab in a simall area of the 1" quadrant. i[his 68108 3s Cec eee tite loop, streak the inoculum back and forth ‘over the first quarter of the agar’ 3 Gen i nd let it cool 4 Ture peteegs ountar loci forthe 2° quaint ard sta sskng = ti ‘ontinue st ‘across a portion of the 1 quadrant 2-3 times.