GERMAN BIOTECH LABORATORY

SPECIFICATION FOR HERB

Department Document No/ Revision Effective date Page No.
QCD No.
GBL/ RM/HB/019/A 01/07/2022 Page 1 of 2
Herb Name ALFA ALFA
Part Used Whole Plant

Tests Acceptance Criteria Reference

Macroscopical Leaf trifoliate, leaflet oblanceolate to narrowly HPI

Identification obovate, 1.5 to 3 cm long; margin serrate, apex
acute. Peduncle erect, about equaling the
subtending leaves, bearing a subglobose to short-
cylindrical head, 1 to 30 cm long. Flower violet, in
axillary raceme of 7 to 10 flowers; 3 to 4 cm long;
calyx five-toothed; corolla papilionaceous; stamens
1, nine united and one free.
Microscopical HPI
Identification

 Leaflet Epidermal cells with wavy anticlinal walls, upper HPI

epidermal cells 14-25-50-70µ, slightly smaller than the
lower epidermal cells which are 20-35-60-100µ.Stomata
on both surfaces, oval, 20-30-40µ long and 10-20-25µ
wide, either anisocytic or anomocytic.
 Trichomes Rare on the upper surface, numerous over the mid-rib, HPI
on the undersurface, consisting of 2 short basal cells,
10-20µ long and a linear terminal cell 350-1120µ long
and 20µ wide, Clavate glandular trichomes 75-100µ
long, occur mostly on the undersurface and the calyx,
usually consisting of a stalk cell, an intermediate and a
terminal cell, 25 wide with a rounded apex and often
divided into 2 to 4 cells
 Lamina Thin, transverse section showing 1-2 layered palisade HPI
and spongy parenchyma; the vascular bundles have
fibers above and below and the cells of the parenchyma
abutting upon the fibers contain prism crystals of
calcium oxalate oxalate 8-15 by 4-8 wide.
 Corolla Epidermis papillose having striated cuticule. Pollen HPI
grains spherical, 30-35 in diameter with 3 pores and 3
germinal furrows, exine smooth and contents granular.

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 GERMAN BIOTECH LABORATORY
SPECIFICATION FOR HERB
Department Document No/ Revision Effective date Page No.
QCD No.
GBL/ RM/HB/019/A 01/07/2022 Page 2 of 2
Herb Name ALFA ALFA
Part Used Whole Plant

 Flower Almost square, epidermal cells elongated in axial HPI

 Young Stem
 Mature Stem
Identification
(B y TLC)
Carry out Co-TLC of Five spots appear at Rf 0.12, 0.20, 0.30, 0.40, and 0.54
chloroform extract of Mother
Tincture on silica gel 'G' pre-
coated plate using n-butanol:
acetic acid: water (4: 1:1v/v)
as sorvent system. After
spraying the plate with
ninhydrin reagent and heating
the plate for 10 minutes at
110°.
direction with two types of epidermal hairs, glandular, 2
or more celled and unicellular trichomes, cortex of
rectangular r oval cells containing starch grains and
crystals of calcium oxalate.
Endodermis usually without casparian bands. Outside HPI
the endodermis is a zone of chlorenchyma, limited
peripherally by a single layer collenchymas except at
the angles of stem.
Vascular bundles collateral, conjoint and open.
Continuous cambial ring is formed which produces
continuous zone of lignified xylem. Parenchymatous
cells of pih disintegrate to form a hollow.
The fluorescence turns greenish on adding a few drops HPI
of 5% sodium hydroxide solution.
HPI
respectively.

Loss on drying As per finding IHS

HPI: HOMEOPATHIC PHARMACOPOEIA OF INDIA

IHS: IN HOUSE SPECIFICATION

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Executive Regulatory Affairs Manager Quality Control Technical Head